AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045277.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCCACCTGGGCCTGATGC+TGG 0.215084 8:-56798131 MsG0880045277.01.T01:CDS
ATTTGTGATATTAGGTCTTT+TGG 0.242921 8:+56797920 None:intergenic
GGTGAAAAGGTGTTTGGGCT+TGG 0.255574 8:-56798086 MsG0880045277.01.T01:CDS
GTGAAAAGGTGTTTGGGCTT+GGG 0.286134 8:-56798085 MsG0880045277.01.T01:CDS
GATGGAGCTGGTGCTGAATT+TGG 0.295928 8:-56797996 MsG0880045277.01.T01:CDS
GCTGGTGGTGAAAAGGTGTT+TGG 0.314923 8:-56798092 MsG0880045277.01.T01:CDS
ATCAAATTATTTGTGATATT+AGG 0.323632 8:+56797912 None:intergenic
TTTGGGCTTGGGCTTGAGCC+TGG 0.327247 8:-56798074 MsG0880045277.01.T01:CDS
TGCTGGAATCTTTGTGTCCA+TGG 0.342466 8:+56798232 None:intergenic
CCCCACCTGGGCCTGATGCT+GGG 0.358406 8:-56798130 MsG0880045277.01.T01:CDS
GAAGTTGAAGGAGTATTTGC+AGG 0.364279 8:-56798047 MsG0880045277.01.T01:CDS
TGTCCCGGTGTGGCCCCACC+TGG 0.378803 8:-56798143 MsG0880045277.01.T01:CDS
GGCTCAATTGTCCCAGCATC+AGG 0.383419 8:+56798119 None:intergenic
GCAGAGGGTGCATCAGCTCC+TGG 0.383493 8:-56798167 MsG0880045277.01.T01:CDS
CTGGTGGTGAAAAGGTGTTT+GGG 0.427241 8:-56798091 MsG0880045277.01.T01:CDS
GGGACAATTGAGCCTGGTGC+TGG 0.434202 8:-56798110 MsG0880045277.01.T01:CDS
GGGGCCACACCGGGACAGCC+AGG 0.442844 8:+56798149 None:intergenic
GATTGTGTGGGTGCTGGTGC+AGG 0.453602 8:-56797966 MsG0880045277.01.T01:CDS
GCATCAGCTCCTGGCTGTCC+CGG 0.460777 8:-56798158 MsG0880045277.01.T01:CDS
CTGGTGCAGGAGCTTGTGCT+AGG 0.467111 8:-56797953 MsG0880045277.01.T01:CDS
GATAGTGATTGTGTGGGTGC+TGG 0.477013 8:-56797972 MsG0880045277.01.T01:CDS
GGCCCAGGTGGGGCCACACC+GGG 0.491499 8:+56798140 None:intergenic
TGAAGGAGTATTTGCAGGAG+AGG 0.493181 8:-56798042 MsG0880045277.01.T01:CDS
GTATTTGCAGGAGAGGGTGT+TGG 0.495133 8:-56798035 MsG0880045277.01.T01:CDS
TCCCAGCATCAGGCCCAGGT+GGG 0.497867 8:+56798129 None:intergenic
GCCTGGTGCTGGTGGTGAAA+AGG 0.501298 8:-56798099 MsG0880045277.01.T01:CDS
GTCCCGGTGTGGCCCCACCT+GGG 0.505740 8:-56798142 MsG0880045277.01.T01:CDS
CCCAGCATCAGGCCCAGGTG+GGG 0.510499 8:+56798130 None:intergenic
GATGCTGGGACAATTGAGCC+TGG 0.515568 8:-56798116 MsG0880045277.01.T01:CDS
GAAGGAGTATTTGCAGGAGA+GGG 0.515675 8:-56798041 MsG0880045277.01.T01:CDS
AGGCCCAGGTGGGGCCACAC+CGG 0.517610 8:+56798139 None:intergenic
CTTGTAGAGGGTGATGGAGC+TGG 0.523360 8:-56798008 MsG0880045277.01.T01:CDS
ATTTGGTGATAGTGATTGTG+TGG 0.527810 8:-56797979 MsG0880045277.01.T01:CDS
ATTGTCCCAGCATCAGGCCC+AGG 0.542663 8:+56798125 None:intergenic
GGTGATCTTGTAGAGGGTGA+TGG 0.551171 8:-56798014 MsG0880045277.01.T01:CDS
GGGTGTTGGTGATCTTGTAG+AGG 0.554648 8:-56798021 MsG0880045277.01.T01:CDS
GAGCCTGGTGAAGAAGTTGA+AGG 0.556070 8:-56798059 MsG0880045277.01.T01:CDS
GTCCCAGCATCAGGCCCAGG+TGG 0.563718 8:+56798128 None:intergenic
AGCTCCTGGCTGTCCCGGTG+TGG 0.567948 8:-56798153 MsG0880045277.01.T01:CDS
ACAATTGAGCCTGGTGCTGG+TGG 0.580825 8:-56798107 MsG0880045277.01.T01:CDS
ACTCCTTCAACTTCTTCACC+AGG 0.604694 8:+56798056 None:intergenic
AGTAACAGCAACAAAAGTGT+TGG 0.609923 8:-56798204 MsG0880045277.01.T01:CDS
GTGATAGAGAATGCAGCAGA+GGG 0.615284 8:-56798182 MsG0880045277.01.T01:CDS
TTTGGTGATAGTGATTGTGT+GGG 0.627176 8:-56797978 MsG0880045277.01.T01:CDS
GGTGTTGGTGATCTTGTAGA+GGG 0.646401 8:-56798020 MsG0880045277.01.T01:CDS
GGTGATAGAGAATGCAGCAG+AGG 0.711837 8:-56798183 MsG0880045277.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATTATTTGTGATATT+AGG + Chr8:56798166-56798185 None:intergenic 15.0%
!! TCAAAAAATATATACAACAT+GGG + Chr8:56798199-56798218 None:intergenic 15.0%
!! CTCAAAAAATATATACAACA+TGG + Chr8:56798200-56798219 None:intergenic 20.0%
!!! CATGTTGTATATATTTTTTG+AGG - Chr8:56798198-56798217 MsG0880045277.01.T01:CDS 20.0%
!! ATTTGTGATATTAGGTCTTT+TGG + Chr8:56798158-56798177 None:intergenic 25.0%
! AGTAACAGCAACAAAAGTGT+TGG - Chr8:56797871-56797890 MsG0880045277.01.T01:CDS 35.0%
! ATTTGGTGATAGTGATTGTG+TGG - Chr8:56798096-56798115 MsG0880045277.01.T01:CDS 35.0%
! TTTGGTGATAGTGATTGTGT+GGG - Chr8:56798097-56798116 MsG0880045277.01.T01:CDS 35.0%
!! GAAGTTGAAGGAGTATTTGC+AGG - Chr8:56798028-56798047 MsG0880045277.01.T01:CDS 40.0%
ACTCCTTCAACTTCTTCACC+AGG + Chr8:56798022-56798041 None:intergenic 45.0%
GTGATAGAGAATGCAGCAGA+GGG - Chr8:56797893-56797912 MsG0880045277.01.T01:CDS 45.0%
!! CTGGTGGTGAAAAGGTGTTT+GGG - Chr8:56797984-56798003 MsG0880045277.01.T01:CDS 45.0%
!! GAAGGAGTATTTGCAGGAGA+GGG - Chr8:56798034-56798053 MsG0880045277.01.T01:CDS 45.0%
!! GGTGTTGGTGATCTTGTAGA+GGG - Chr8:56798055-56798074 MsG0880045277.01.T01:CDS 45.0%
!! GTGAAAAGGTGTTTGGGCTT+GGG - Chr8:56797990-56798009 MsG0880045277.01.T01:CDS 45.0%
!! GTTGCTGTTACTGCTTTTGC+TGG + Chr8:56797863-56797882 None:intergenic 45.0%
!! TGAAGGAGTATTTGCAGGAG+AGG - Chr8:56798033-56798052 MsG0880045277.01.T01:CDS 45.0%
GAGCCTGGTGAAGAAGTTGA+AGG - Chr8:56798016-56798035 MsG0880045277.01.T01:CDS 50.0%
GATAGTGATTGTGTGGGTGC+TGG - Chr8:56798103-56798122 MsG0880045277.01.T01:CDS 50.0%
GGTGATAGAGAATGCAGCAG+AGG - Chr8:56797892-56797911 MsG0880045277.01.T01:CDS 50.0%
GGTGATCTTGTAGAGGGTGA+TGG - Chr8:56798061-56798080 MsG0880045277.01.T01:CDS 50.0%
! GATGGAGCTGGTGCTGAATT+TGG - Chr8:56798079-56798098 MsG0880045277.01.T01:CDS 50.0%
! GTATTTGCAGGAGAGGGTGT+TGG - Chr8:56798040-56798059 MsG0880045277.01.T01:CDS 50.0%
!! GCTGGTGGTGAAAAGGTGTT+TGG - Chr8:56797983-56798002 MsG0880045277.01.T01:CDS 50.0%
!! GGGTGTTGGTGATCTTGTAG+AGG - Chr8:56798054-56798073 MsG0880045277.01.T01:CDS 50.0%
!! GGTGAAAAGGTGTTTGGGCT+TGG - Chr8:56797989-56798008 MsG0880045277.01.T01:CDS 50.0%
GATGCTGGGACAATTGAGCC+TGG - Chr8:56797959-56797978 MsG0880045277.01.T01:CDS 55.0%
GGCTCAATTGTCCCAGCATC+AGG + Chr8:56797959-56797978 None:intergenic 55.0%
! ACAATTGAGCCTGGTGCTGG+TGG - Chr8:56797968-56797987 MsG0880045277.01.T01:CDS 55.0%
! ACCTTTTCACCACCAGCACC+AGG + Chr8:56797980-56797999 None:intergenic 55.0%
!! CTTGTAGAGGGTGATGGAGC+TGG - Chr8:56798067-56798086 MsG0880045277.01.T01:CDS 55.0%
ATTGTCCCAGCATCAGGCCC+AGG + Chr8:56797953-56797972 None:intergenic 60.0%
CTGGTGCAGGAGCTTGTGCT+AGG - Chr8:56798122-56798141 MsG0880045277.01.T01:CDS 60.0%
GGGACAATTGAGCCTGGTGC+TGG - Chr8:56797965-56797984 MsG0880045277.01.T01:CDS 60.0%
TTTGGGCTTGGGCTTGAGCC+TGG - Chr8:56798001-56798020 MsG0880045277.01.T01:CDS 60.0%
!! GATTGTGTGGGTGCTGGTGC+AGG - Chr8:56798109-56798128 MsG0880045277.01.T01:CDS 60.0%
!! GCCTGGTGCTGGTGGTGAAA+AGG - Chr8:56797976-56797995 MsG0880045277.01.T01:CDS 60.0%
GCATCAGCTCCTGGCTGTCC+CGG - Chr8:56797917-56797936 MsG0880045277.01.T01:CDS 65.0%
TCCCAGCATCAGGCCCAGGT+GGG + Chr8:56797949-56797968 None:intergenic 65.0%
! GCAGAGGGTGCATCAGCTCC+TGG - Chr8:56797908-56797927 MsG0880045277.01.T01:CDS 65.0%
AGCTCCTGGCTGTCCCGGTG+TGG - Chr8:56797922-56797941 MsG0880045277.01.T01:CDS 70.0%
CCCAGCATCAGGCCCAGGTG+GGG + Chr8:56797948-56797967 None:intergenic 70.0%
CCCCACCTGGGCCTGATGCT+GGG - Chr8:56797945-56797964 MsG0880045277.01.T01:CDS 70.0%
GTCCCAGCATCAGGCCCAGG+TGG + Chr8:56797950-56797969 None:intergenic 70.0%
AGGCCCAGGTGGGGCCACAC+CGG + Chr8:56797939-56797958 None:intergenic 75.0%
GCCCCACCTGGGCCTGATGC+TGG - Chr8:56797944-56797963 MsG0880045277.01.T01:CDS 75.0%
GTCCCGGTGTGGCCCCACCT+GGG - Chr8:56797933-56797952 MsG0880045277.01.T01:CDS 75.0%
TGTCCCGGTGTGGCCCCACC+TGG - Chr8:56797932-56797951 MsG0880045277.01.T01:CDS 75.0%
! GGCCCAGGTGGGGCCACACC+GGG + Chr8:56797938-56797957 None:intergenic 80.0%
! GGGGCCACACCGGGACAGCC+AGG + Chr8:56797929-56797948 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr8 gene 56797845 56798252 56797845 ID=MsG0880045277.01;Name=MsG0880045277.01
Chr8 mRNA 56797845 56798252 56797845 ID=MsG0880045277.01.T01;Parent=MsG0880045277.01;Name=MsG0880045277.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|135
Chr8 exon 56797845 56798252 56797845 ID=MsG0880045277.01.T01:exon:1175;Parent=MsG0880045277.01.T01
Chr8 CDS 56797845 56798252 56797845 ID=MsG0880045277.01.T01:cds;Parent=MsG0880045277.01.T01
Gene Sequence

>MsG0880045277.01.T01

ATGGACACAAAGATTCCAGCAAAAGCAGTAACAGCAACAAAAGTGTTGGTGATAGAGAATGCAGCAGAGGGTGCATCAGCTCCTGGCTGTCCCGGTGTGGCCCCACCTGGGCCTGATGCTGGGACAATTGAGCCTGGTGCTGGTGGTGAAAAGGTGTTTGGGCTTGGGCTTGAGCCTGGTGAAGAAGTTGAAGGAGTATTTGCAGGAGAGGGTGTTGGTGATCTTGTAGAGGGTGATGGAGCTGGTGCTGAATTTGGTGATAGTGATTGTGTGGGTGCTGGTGCAGGAGCTTGTGCTAGGCATGATGTAGCCAAAAGACCTAATATCACAAATAATTTGATTGAAGAAAAACCCATGTTGTATATATTTTTTGAGGTAGAAGAATGTTTAATTAGAACTAAGAAGTGA

Protein sequence

>MsG0880045277.01.T01

MDTKIPAKAVTATKVLVIENAAEGASAPGCPGVAPPGPDAGTIEPGAGGEKVFGLGLEPGEEVEGVFAGEGVGDLVEGDGAGAEFGDSDCVGAGAGACARHDVAKRPNITNNLIEEKPMLYIFFEVEECLIRTKK*