AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004184.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004184.01.T02 MTR_1g076700 97.849 93 2 0 1 93 1 93 2.52e-59 193
MsG0180004184.01.T02 MTR_6g061690 79.787 94 18 1 1 93 1 94 9.91e-45 153
MsG0180004184.01.T02 MTR_5g093860 61.842 76 29 0 12 87 12 87 2.86e-27 103
MsG0180004184.01.T02 MTR_3g085440 57.143 77 33 0 13 89 17 93 1.04e-25 99.0
MsG0180004184.01.T02 MTR_3g085440 57.143 77 33 0 13 89 17 93 1.78e-25 99.0
MsG0180004184.01.T02 MTR_7g075220 51.724 87 42 0 1 87 311 397 5.18e-25 97.4
MsG0180004184.01.T02 MTR_8g041400 53.012 83 39 0 9 91 8 90 1.17e-24 96.7
MsG0180004184.01.T02 MTR_1g038840 57.534 73 31 0 14 86 15 87 1.60e-23 93.2
MsG0180004184.01.T02 MTR_4g077600 77.778 54 12 0 24 77 185 238 6.04e-22 86.3
MsG0180004184.01.T02 MTR_8g023220 46.988 83 42 2 9 89 8 90 1.68e-16 73.2
MsG0180004184.01.T02 MTR_1g038840 55.319 47 21 0 40 86 1 47 9.54e-11 57.0
MsG0180004184.01.T01 MTR_1g076700 97.849 93 2 0 1 93 1 93 2.52e-59 193
MsG0180004184.01.T01 MTR_6g061690 79.787 94 18 1 1 93 1 94 9.91e-45 153
MsG0180004184.01.T01 MTR_5g093860 61.842 76 29 0 12 87 12 87 2.86e-27 103
MsG0180004184.01.T01 MTR_3g085440 57.143 77 33 0 13 89 17 93 1.04e-25 99.0
MsG0180004184.01.T01 MTR_3g085440 57.143 77 33 0 13 89 17 93 1.78e-25 99.0
MsG0180004184.01.T01 MTR_7g075220 51.724 87 42 0 1 87 311 397 5.18e-25 97.4
MsG0180004184.01.T01 MTR_8g041400 53.012 83 39 0 9 91 8 90 1.17e-24 96.7
MsG0180004184.01.T01 MTR_1g038840 57.534 73 31 0 14 86 15 87 1.60e-23 93.2
MsG0180004184.01.T01 MTR_4g077600 77.778 54 12 0 24 77 185 238 6.04e-22 86.3
MsG0180004184.01.T01 MTR_8g023220 46.988 83 42 2 9 89 8 90 1.68e-16 73.2
MsG0180004184.01.T01 MTR_1g038840 55.319 47 21 0 40 86 1 47 9.54e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004184.01.T02 AT5G51600 81.720 93 17 0 1 93 1 93 1.68e-40 141
MsG0180004184.01.T02 AT5G55230 52.564 78 37 0 12 89 12 89 1.18e-24 96.7
MsG0180004184.01.T02 AT5G55230 52.564 78 37 0 12 89 12 89 1.31e-24 96.3
MsG0180004184.01.T02 AT5G55230 52.564 78 37 0 12 89 12 89 1.31e-24 96.3
MsG0180004184.01.T02 AT1G14690 54.667 75 34 0 11 85 13 87 4.71e-24 94.7
MsG0180004184.01.T02 AT1G14690 54.667 75 34 0 11 85 13 87 4.71e-24 94.7
MsG0180004184.01.T02 AT1G27920 55.000 80 36 0 10 89 14 93 9.18e-24 93.6
MsG0180004184.01.T02 AT1G27920 55.844 77 34 0 13 89 17 93 1.29e-23 93.6
MsG0180004184.01.T02 AT4G26760 51.316 76 37 0 12 87 12 87 7.87e-23 91.3
MsG0180004184.01.T02 AT2G01910 54.667 75 34 0 11 85 13 87 4.88e-22 89.4
MsG0180004184.01.T02 AT2G38720 55.072 69 31 0 19 87 12 80 1.88e-21 87.4
MsG0180004184.01.T02 AT3G60840 50.000 82 41 0 9 90 9 90 2.32e-21 87.4
MsG0180004184.01.T02 AT5G62250 50.000 80 40 0 10 89 4 83 2.31e-13 64.3
MsG0180004184.01.T02 AT5G62250 50.000 80 40 0 10 89 4 83 2.31e-13 64.3
MsG0180004184.01.T01 AT5G51600 81.720 93 17 0 1 93 1 93 1.68e-40 141
MsG0180004184.01.T01 AT5G55230 52.564 78 37 0 12 89 12 89 1.18e-24 96.7
MsG0180004184.01.T01 AT5G55230 52.564 78 37 0 12 89 12 89 1.31e-24 96.3
MsG0180004184.01.T01 AT5G55230 52.564 78 37 0 12 89 12 89 1.31e-24 96.3
MsG0180004184.01.T01 AT1G14690 54.667 75 34 0 11 85 13 87 4.71e-24 94.7
MsG0180004184.01.T01 AT1G14690 54.667 75 34 0 11 85 13 87 4.71e-24 94.7
MsG0180004184.01.T01 AT1G27920 55.000 80 36 0 10 89 14 93 9.18e-24 93.6
MsG0180004184.01.T01 AT1G27920 55.844 77 34 0 13 89 17 93 1.29e-23 93.6
MsG0180004184.01.T01 AT4G26760 51.316 76 37 0 12 87 12 87 7.87e-23 91.3
MsG0180004184.01.T01 AT2G01910 54.667 75 34 0 11 85 13 87 4.88e-22 89.4
MsG0180004184.01.T01 AT2G38720 55.072 69 31 0 19 87 12 80 1.88e-21 87.4
MsG0180004184.01.T01 AT3G60840 50.000 82 41 0 9 90 9 90 2.32e-21 87.4
MsG0180004184.01.T01 AT5G62250 50.000 80 40 0 10 89 4 83 2.31e-13 64.3
MsG0180004184.01.T01 AT5G62250 50.000 80 40 0 10 89 4 83 2.31e-13 64.3

Find 2 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCATTTCTTTCCTAAAT+AGG 0.174626 1:+74642018 MsG0180004184.01.T01:five_prime_UTR
CGAAATGTACTGACCTATTT+AGG 0.223066 1:-74642031 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTAAATAAATTGAATTTA+GGG + Chr1:74642266-74642285 MsG0180004184.01.T01:intron 10.0%
!! TAGTTAAATAAATTGAATTT+AGG + Chr1:74642265-74642284 MsG0180004184.01.T01:intron 10.0%
!!! AATTCATTTTTTAACAATTT+AGG + Chr1:74642229-74642248 MsG0180004184.01.T01:intron 10.0%
!!! ATTCATTTTTTAACAATTTA+GGG + Chr1:74642230-74642249 MsG0180004184.01.T01:intron 10.0%
!! AAATAGAAAGTTTGTATAAA+AGG - Chr1:74642105-74642124 None:intergenic 15.0%
!! ACTTGTTTATTAGATAATTT+GGG + Chr1:74642579-74642598 MsG0180004184.01.T01:intron 15.0%
!!! AACTTGTTTATTAGATAATT+TGG + Chr1:74642578-74642597 MsG0180004184.01.T01:intron 15.0%
!! AATGAATTGTAACTCAAATT+TGG - Chr1:74642217-74642236 None:intergenic 20.0%
!! ATGAATTGTAACTCAAATTT+GGG - Chr1:74642216-74642235 None:intergenic 20.0%
!!! CTTTTTCCTTAAAACTAAAA+CGG + Chr1:74641895-74641914 MsG0180004184.01.T01:five_prime_UTR 20.0%
! TCTTCATTTCTTTCCTAAAT+AGG + Chr1:74642018-74642037 MsG0180004184.01.T01:five_prime_UTR 25.0%
! TGAATATAGTTAAGATTCGT+GGG - Chr1:74642068-74642087 None:intergenic 25.0%
! TGAATTGTAACTCAAATTTG+GGG - Chr1:74642215-74642234 None:intergenic 25.0%
! TTGAATATAGTTAAGATTCG+TGG - Chr1:74642069-74642088 None:intergenic 25.0%
!!! AATTTTGAAGTGATTGTGTT+TGG + Chr1:74642386-74642405 MsG0180004184.01.T01:intron 25.0%
!!! CGTTTTAGTTTTAAGGAAAA+AGG - Chr1:74641897-74641916 None:intergenic 25.0%
AATTGTAACTCAAATTTGGG+GGG - Chr1:74642213-74642232 None:intergenic 30.0%
ACACATTTAAGCGATTAAAG+AGG - Chr1:74642327-74642346 None:intergenic 30.0%
CTTTAATCGCTTAAATGTGT+TGG + Chr1:74642327-74642346 MsG0180004184.01.T01:intron 30.0%
GAATTGTAACTCAAATTTGG+GGG - Chr1:74642214-74642233 None:intergenic 30.0%
TAATGTGTGATTGAGAGAAA+TGG - Chr1:74642166-74642185 None:intergenic 30.0%
! ATAAGTCTAGAGCTCAATTA+AGG + Chr1:74642698-74642717 MsG0180004184.01.T01:CDS 30.0%
!!! ATGTAGCCGTTTTAGTTTTA+AGG - Chr1:74641904-74641923 None:intergenic 30.0%
AAGAGATCCACAAGTTGTTT+CGG - Chr1:74642452-74642471 None:intergenic 35.0%
ATCTCTTCTGTATGAACTTC+AGG + Chr1:74642465-74642484 MsG0180004184.01.T01:CDS 35.0%
CAATCACTTCAAAATTCAGC+TGG - Chr1:74642382-74642401 None:intergenic 35.0%
GTACTAACATAGATCTTAGC+TGG - Chr1:74642144-74642163 None:intergenic 35.0%
! CGAAATGTACTGACCTATTT+AGG - Chr1:74642034-74642053 None:intergenic 35.0%
! TAGATAATTTGGGACGAAGT+TGG + Chr1:74642589-74642608 MsG0180004184.01.T01:intron 35.0%
AACAGCATTCTGTCTCTATC+AGG - Chr1:74642625-74642644 None:intergenic 40.0%
ATAACCTGTCTAATGTGCAC+AGG - Chr1:74642784-74642803 None:intergenic 40.0%
TTAAGATTCGTGGGAATCTG+AGG - Chr1:74642059-74642078 None:intergenic 40.0%
AGTGTCTTGAAGTGTACCGA+AGG + Chr1:74642659-74642678 MsG0180004184.01.T01:CDS 45.0%
GCAGCTATCTGTTCTGCAAT+GGG + Chr1:74642751-74642770 MsG0180004184.01.T01:CDS 45.0%
TCTTGAAGTGTACCGAAGGA+AGG + Chr1:74642663-74642682 MsG0180004184.01.T01:CDS 45.0%
TGCAGCTATCTGTTCTGCAA+TGG + Chr1:74642750-74642769 MsG0180004184.01.T01:CDS 45.0%
! AAGTTGTTTCGGCTTGCGAA+AGG - Chr1:74642441-74642460 None:intergenic 45.0%
! AGTTGTTTCGGCTTGCGAAA+GGG - Chr1:74642440-74642459 None:intergenic 45.0%
! GTCTAGAGCTCAATTAAGGC+AGG + Chr1:74642702-74642721 MsG0180004184.01.T01:CDS 45.0%
GTACCGAAGGAAGGTAGATC+AGG + Chr1:74642672-74642691 MsG0180004184.01.T01:CDS 50.0%
TCGCAAGCCGAAACAACTTG+TGG + Chr1:74642442-74642461 MsG0180004184.01.T01:CDS 50.0%
TCGCCTGATCTACCTTCCTT+CGG - Chr1:74642678-74642697 None:intergenic 50.0%
! AATTTGGGACGAAGTTGGCG+AGG + Chr1:74642594-74642613 MsG0180004184.01.T01:CDS 50.0%
! GTTGTTTCGGCTTGCGAAAG+GGG - Chr1:74642439-74642458 None:intergenic 50.0%
! TGCGAAAGGGGATCGTTTTG+AGG - Chr1:74642427-74642446 None:intergenic 50.0%
GCGACCTGTGCACATTAGAC+AGG + Chr1:74642777-74642796 MsG0180004184.01.T01:CDS 55.0%
! GCAGGCGATTGCTGATTGTG+AGG + Chr1:74642720-74642739 MsG0180004184.01.T01:CDS 55.0%
! GGCGATTGCTGATTGTGAGG+CGG + Chr1:74642723-74642742 MsG0180004184.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 74641869 74642810 74641869 ID=MsG0180004184.01;Name=MsG0180004184.01
Chr1 mRNA 74641869 74642810 74641869 ID=MsG0180004184.01.T01;Parent=MsG0180004184.01;Name=MsG0180004184.01.T01;_AED=0.49;_eAED=0.50;_QI=184|1|0.66|1|0.5|0.33|3|0|96
Chr1 exon 74641869 74642049 74641869 ID=MsG0180004184.01.T01:exon:5620;Parent=MsG0180004184.01.T01
Chr1 five_prime_UTR 74641869 74642049 74641869 ID=MsG0180004184.01.T01:five_prime_utr;Parent=MsG0180004184.01.T01
Chr1 five_prime_UTR 74642412 74642414 74642412 ID=MsG0180004184.01.T01:five_prime_utr;Parent=MsG0180004184.01.T01
Chr1 CDS 74642415 74642486 74642415 ID=MsG0180004184.01.T01:cds;Parent=MsG0180004184.01.T01
Chr1 CDS 74642592 74642810 74642592 ID=MsG0180004184.01.T01:cds;Parent=MsG0180004184.01.T01
Chr1 mRNA 74641935 74642810 74641935 ID=MsG0180004184.01.T02;Parent=MsG0180004184.01;Name=MsG0180004184.01.T02;_AED=0.49;_eAED=0.50;_QI=108|1|0.66|1|0.5|0.33|3|0|96
Chr1 exon 74641935 74642039 74641935 ID=MsG0180004184.01.T02:exon:5623;Parent=MsG0180004184.01.T02
Chr1 five_prime_UTR 74641935 74642039 74641935 ID=MsG0180004184.01.T02:five_prime_utr;Parent=MsG0180004184.01.T02
Chr1 five_prime_UTR 74642412 74642414 74642412 ID=MsG0180004184.01.T02:five_prime_utr;Parent=MsG0180004184.01.T02
Chr1 CDS 74642415 74642486 74642415 ID=MsG0180004184.01.T02:cds;Parent=MsG0180004184.01.T02
Chr1 CDS 74642592 74642810 74642592 ID=MsG0180004184.01.T02:cds;Parent=MsG0180004184.01.T02
Gene Sequence

>MsG0180004184.01.T02

ATGTCTAATCCTCAAAACGATCCCCTTTCGCAAGCCGAAACAACTTGTGGATCTCTTCTGTATGAACTTCAGATAATTTGGGACGAAGTTGGCGAGGCTGAGCCTGATAGAGACAGAATGCTGTTTGAGCTTGAACAAGAGTGTCTTGAAGTGTACCGAAGGAAGGTAGATCAGGCGAATAAGTCTAGAGCTCAATTAAGGCAGGCGATTGCTGATTGTGAGGCGGAACTTGCAGCTATCTGTTCTGCAATGGGAGAGCGACCTGTGCACATTAGACAGGTTATATTTTAA

>MsG0180004184.01.T01

ATGTCTAATCCTCAAAACGATCCCCTTTCGCAAGCCGAAACAACTTGTGGATCTCTTCTGTATGAACTTCAGATAATTTGGGACGAAGTTGGCGAGGCTGAGCCTGATAGAGACAGAATGCTGTTTGAGCTTGAACAAGAGTGTCTTGAAGTGTACCGAAGGAAGGTAGATCAGGCGAATAAGTCTAGAGCTCAATTAAGGCAGGCGATTGCTGATTGTGAGGCGGAACTTGCAGCTATCTGTTCTGCAATGGGAGAGCGACCTGTGCACATTAGACAGGTTATATTTTAA

Protein sequence

>MsG0180004184.01.T02

MSNPQNDPLSQAETTCGSLLYELQIIWDEVGEAEPDRDRMLFELEQECLEVYRRKVDQANKSRAQLRQAIADCEAELAAICSAMGERPVHIRQVIF*

>MsG0180004184.01.T01

MSNPQNDPLSQAETTCGSLLYELQIIWDEVGEAEPDRDRMLFELEQECLEVYRRKVDQANKSRAQLRQAIADCEAELAAICSAMGERPVHIRQVIF*