Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025515.01.T01 | XP_003612880.1 | 98.885 | 269 | 3 | 0 | 1 | 269 | 1 | 269 | 0 | 546 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025515.01.T01 | Q9FIQ5 | 67.286 | 269 | 88 | 0 | 1 | 269 | 1 | 269 | 2.17E-127 | 365 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025515.01.T01 | G7KDS1 | 98.885 | 269 | 3 | 0 | 1 | 269 | 1 | 269 | 0.0 | 546 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048701.01 | MsG0580025515.01 | 0.862147 | 6.226459e-64 | 1.804975e-60 |
| MsG0080048781.01 | MsG0580025515.01 | 0.823260 | 1.523970e-53 | 1.305592e-50 |
| MsG0080048996.01 | MsG0580025515.01 | 0.813344 | 2.684424e-51 | 1.748595e-48 |
| MsG0180000076.01 | MsG0580025515.01 | 0.840095 | 1.069007e-57 | 1.506791e-54 |
| MsG0180000111.01 | MsG0580025515.01 | 0.854226 | 1.417599e-61 | 3.144366e-58 |
| MsG0180000353.01 | MsG0580025515.01 | 0.803338 | 3.655525e-49 | 1.832232e-46 |
| MsG0180000360.01 | MsG0580025515.01 | 0.848316 | 6.624351e-60 | 1.210856e-56 |
| MsG0180000373.01 | MsG0580025515.01 | 0.841850 | 3.699834e-58 | 5.506863e-55 |
| MsG0180002211.01 | MsG0580025515.01 | 0.825158 | 5.459271e-54 | 4.936918e-51 |
| MsG0180002421.01 | MsG0580025515.01 | 0.830618 | 2.653924e-55 | 2.810866e-52 |
| MsG0180002583.01 | MsG0580025515.01 | 0.801417 | 9.094555e-49 | 4.340877e-46 |
| MsG0180003360.01 | MsG0580025515.01 | 0.813871 | 2.055711e-51 | 1.358303e-48 |
| MsG0180003427.01 | MsG0580025515.01 | 0.839092 | 1.948342e-57 | 2.662208e-54 |
| MsG0180003485.01 | MsG0580025515.01 | 0.841885 | 3.623053e-58 | 5.398292e-55 |
| MsG0180003796.01 | MsG0580025515.01 | 0.876754 | 1.086121e-68 | 5.327478e-65 |
| MsG0180004126.01 | MsG0580025515.01 | 0.816054 | 6.738774e-52 | 4.725390e-49 |
| MsG0180004806.01 | MsG0580025515.01 | 0.816151 | 6.411547e-52 | 4.507666e-49 |
| MsG0180005002.01 | MsG0580025515.01 | 0.832524 | 9.002651e-56 | 1.008777e-52 |
| MsG0180005435.01 | MsG0580025515.01 | 0.816984 | 4.171979e-52 | 3.000922e-49 |
| MsG0180005835.01 | MsG0580025515.01 | 0.872624 | 2.756324e-67 | 1.161378e-63 |
| MsG0280006310.01 | MsG0580025515.01 | 0.835864 | 1.309256e-56 | 1.621544e-53 |
| MsG0280006311.01 | MsG0580025515.01 | 0.827970 | 1.165314e-54 | 1.142653e-51 |
| MsG0280010779.01 | MsG0580025515.01 | 0.801408 | 9.134217e-49 | 4.358752e-46 |
| MsG0280010795.01 | MsG0580025515.01 | 0.807153 | 5.807714e-50 | 3.210944e-47 |
| MsG0280010868.01 | MsG0580025515.01 | 0.814280 | 1.669344e-51 | 1.115351e-48 |
| MsG0380012325.01 | MsG0580025515.01 | 0.805521 | 1.282314e-49 | 6.798405e-47 |
| MsG0380012472.01 | MsG0580025515.01 | 0.817164 | 3.800503e-52 | 2.747028e-49 |
| MsG0380012788.01 | MsG0580025515.01 | 0.815261 | 1.012202e-51 | 6.944518e-49 |
| MsG0380012808.01 | MsG0580025515.01 | 0.815897 | 7.303613e-52 | 5.098974e-49 |
| MsG0380014594.01 | MsG0580025515.01 | 0.831250 | 1.856432e-55 | 2.003155e-52 |
| MsG0380014754.01 | MsG0580025515.01 | 0.837909 | 3.934839e-57 | 5.185830e-54 |
| MsG0480018526.01 | MsG0580025515.01 | 0.831604 | 1.519175e-55 | 1.656844e-52 |
| MsG0480018918.01 | MsG0580025515.01 | 0.805180 | 1.511775e-49 | 7.945278e-47 |
| MsG0480019588.01 | MsG0580025515.01 | 0.837622 | 4.661808e-57 | 6.090634e-54 |
| MsG0480019635.01 | MsG0580025515.01 | 0.902045 | 1.447456e-78 | 2.011572e-74 |
| MsG0480019699.01 | MsG0580025515.01 | 0.870126 | 1.846416e-66 | 7.101712e-63 |
| MsG0480020263.01 | MsG0580025515.01 | 0.855286 | 6.983412e-62 | 1.604215e-58 |
| MsG0480020965.01 | MsG0580025515.01 | 0.873726 | 1.176293e-67 | 5.158303e-64 |
| MsG0480021013.01 | MsG0580025515.01 | 0.869561 | 2.824383e-66 | 1.064636e-62 |
| MsG0480021156.01 | MsG0580025515.01 | 0.853432 | 2.399345e-61 | 5.183688e-58 |
| MsG0480022313.01 | MsG0580025515.01 | 0.813528 | 2.445704e-51 | 1.601128e-48 |
| MsG0480022331.01 | MsG0580025515.01 | 0.870805 | 1.105632e-66 | 4.358111e-63 |
| MsG0480022873.01 | MsG0580025515.01 | 0.860194 | 2.447411e-63 | 6.634993e-60 |
| MsG0480022998.01 | MsG0580025515.01 | 0.803488 | 3.403517e-49 | 1.712568e-46 |
| MsG0480023194.01 | MsG0580025515.01 | 0.840603 | 7.873762e-58 | 1.127242e-54 |
| MsG0480023296.01 | MsG0580025515.01 | 0.830080 | 3.591434e-55 | 3.744654e-52 |
| MsG0480023458.01 | MsG0580025515.01 | 0.810918 | 9.075428e-51 | 5.541545e-48 |
| MsG0480023532.01 | MsG0580025515.01 | 0.832137 | 1.122419e-55 | 1.243330e-52 |
| MsG0480023672.01 | MsG0580025515.01 | 0.830551 | 2.755944e-55 | 2.913028e-52 |
| MsG0580024657.01 | MsG0580025515.01 | 0.804987 | 1.658734e-49 | 8.674634e-47 |
| MsG0580024758.01 | MsG0580025515.01 | 0.804004 | 2.659120e-49 | 1.355899e-46 |
| MsG0580024787.01 | MsG0580025515.01 | 0.806793 | 6.921283e-50 | 3.791073e-47 |
| MsG0580024954.01 | MsG0580025515.01 | 0.827482 | 1.527104e-54 | 1.476190e-51 |
| MsG0580025093.01 | MsG0580025515.01 | 0.833409 | 5.423647e-56 | 6.240597e-53 |
| MsG0580025515.01 | MsG0580025766.01 | 0.801747 | 7.781611e-49 | 3.745270e-46 |
| MsG0580025515.01 | MsG0580025775.01 | 0.815891 | 7.326023e-52 | 5.113753e-49 |
| MsG0580025515.01 | MsG0580025822.01 | 0.841724 | 3.994083e-58 | 5.920806e-55 |
| MsG0580025515.01 | MsG0580026186.01 | 0.819710 | 1.006640e-52 | 7.805652e-50 |
| MsG0580025515.01 | MsG0580027049.01 | 0.827615 | 1.418656e-54 | 1.376469e-51 |
| MsG0580025515.01 | MsG0580028756.01 | 0.827620 | 1.415124e-54 | 1.373213e-51 |
| MsG0580025515.01 | MsG0580028923.01 | 0.804300 | 2.307951e-49 | 1.185773e-46 |
| MsG0580025515.01 | MsG0580029123.01 | 0.801647 | 8.157809e-49 | 3.916363e-46 |
| MsG0580025515.01 | MsG0580029298.01 | 0.833950 | 3.972902e-56 | 4.646578e-53 |
| MsG0580025515.01 | MsG0680032773.01 | 0.800603 | 1.334021e-48 | 6.237536e-46 |
| MsG0580025515.01 | MsG0680033696.01 | 0.803848 | 2.866272e-49 | 1.455680e-46 |
| MsG0580025515.01 | MsG0780036360.01 | 0.819666 | 1.030223e-52 | 7.978479e-50 |
| MsG0580025515.01 | MsG0780038402.01 | 0.811148 | 8.093712e-51 | 4.971815e-48 |
| MsG0580025515.01 | MsG0780039861.01 | 0.845846 | 3.147327e-59 | 5.315254e-56 |
| MsG0580025515.01 | MsG0780039878.01 | 0.841297 | 5.175687e-58 | 7.570800e-55 |
| MsG0580025515.01 | MsG0780040533.01 | 0.801984 | 6.954950e-49 | 3.367604e-46 |
| MsG0580025515.01 | MsG0780040828.01 | 0.831121 | 1.996977e-55 | 2.146739e-52 |
| MsG0580025515.01 | MsG0780040896.01 | 0.825361 | 4.888530e-54 | 4.445897e-51 |
| MsG0580025515.01 | MsG0780041063.01 | 0.827366 | 1.628136e-54 | 1.568743e-51 |
| MsG0580025515.01 | MsG0780041132.01 | 0.829929 | 3.908954e-55 | 4.058435e-52 |
| MsG0580025515.01 | MsG0780041143.01 | 0.822101 | 2.835630e-53 | 2.350629e-50 |
| MsG0580025515.01 | MsG0780041271.01 | 0.834666 | 2.626261e-56 | 3.137754e-53 |
| MsG0580025515.01 | MsG0780041340.01 | 0.800050 | 1.728137e-48 | 7.967841e-46 |
| MsG0580025515.01 | MsG0780041756.01 | 0.803365 | 3.609152e-49 | 1.810213e-46 |
| MsG0580025515.01 | MsG0880041837.01 | 0.824420 | 8.149583e-54 | 7.214340e-51 |
| MsG0580025515.01 | MsG0880042366.01 | 0.806048 | 9.936262e-50 | 5.339940e-47 |
| MsG0580025515.01 | MsG0880042845.01 | 0.813581 | 2.381004e-51 | 1.560941e-48 |
| MsG0580025515.01 | MsG0880042906.01 | 0.818014 | 2.444569e-52 | 1.808583e-49 |
| MsG0580025515.01 | MsG0880042927.01 | 0.842690 | 2.216684e-58 | 3.387366e-55 |
| MsG0580025515.01 | MsG0880042929.01 | 0.818737 | 1.676756e-52 | 1.265496e-49 |
| MsG0580025515.01 | MsG0880043299.01 | 0.812035 | 5.191182e-51 | 3.264676e-48 |
| MsG0580025515.01 | MsG0880043516.01 | 0.805292 | 1.432049e-49 | 7.547946e-47 |
| MsG0580025515.01 | MsG0880043951.01 | 0.835121 | 2.017997e-56 | 2.444121e-53 |
| MsG0580025515.01 | MsG0880044984.01 | 0.806992 | 6.282248e-50 | 3.458731e-47 |
| MsG0580025515.01 | MsG0880044989.01 | 0.815755 | 7.858001e-52 | 5.465334e-49 |
| MsG0580025515.01 | MsG0880046431.01 | 0.820844 | 5.532077e-53 | 4.427028e-50 |
| MsG0580025515.01 | MsG0880046993.01 | 0.803773 | 2.970362e-49 | 1.505591e-46 |
| MsG0580025515.01 | MsG0880047223.01 | 0.881187 | 2.957118e-70 | 1.715644e-66 |
| MsG0580025515.01 | MsG0880047226.01 | 0.823227 | 1.551176e-53 | 1.327666e-50 |
| MsG0580025515.01 | MsG0880047431.01 | 0.818603 | 1.798052e-52 | 1.352016e-49 |
| MsG0580025515.01 | MsG0880047466.01 | 0.817428 | 3.314554e-52 | 2.413287e-49 |
| MsG0580025515.01 | MsG0880047649.01 | 0.803205 | 3.894987e-49 | 1.945647e-46 |
| MsG0380015161.01 | MsG0580025515.01 | 0.873030 | 2.015935e-67 | 8.619585e-64 |
| MsG0380015548.01 | MsG0580025515.01 | 0.835995 | 1.212916e-56 | 1.508233e-53 |
| MsG0380015552.01 | MsG0580025515.01 | 0.822618 | 2.150883e-53 | 1.809755e-50 |
| MsG0380016140.01 | MsG0580025515.01 | 0.812895 | 3.367351e-51 | 2.166936e-48 |
| MsG0380016703.01 | MsG0580025515.01 | 0.833219 | 6.046223e-56 | 6.917704e-53 |
| MsG0380017091.01 | MsG0580025515.01 | 0.801652 | 8.138889e-49 | 3.907790e-46 |
| MsG0380017164.01 | MsG0580025515.01 | 0.825679 | 4.109610e-54 | 3.771668e-51 |
| MsG0380017239.01 | MsG0580025515.01 | 0.870222 | 1.717851e-66 | 6.630907e-63 |
| MsG0380017361.01 | MsG0580025515.01 | 0.830291 | 3.189154e-55 | 3.345758e-52 |
| MsG0380017819.01 | MsG0580025515.01 | 0.808039 | 3.766931e-50 | 2.131259e-47 |
| MsG0480018397.01 | MsG0580025515.01 | 0.839638 | 1.405656e-57 | 1.953089e-54 |
| MsG0280006540.01 | MsG0580025515.01 | 0.861140 | 1.264757e-63 | 3.542072e-60 |
| MsG0280006563.01 | MsG0580025515.01 | 0.826816 | 2.203632e-54 | 2.089111e-51 |
| MsG0280006884.01 | MsG0580025515.01 | 0.820037 | 8.476718e-53 | 6.633422e-50 |
| MsG0280007359.01 | MsG0580025515.01 | 0.834436 | 3.000125e-56 | 3.560274e-53 |
| MsG0280007761.01 | MsG0580025515.01 | 0.870849 | 1.069092e-66 | 4.221232e-63 |
| MsG0280008280.01 | MsG0580025515.01 | 0.826452 | 2.691590e-54 | 2.525275e-51 |
| MsG0280008371.01 | MsG0580025515.01 | 0.828519 | 8.592756e-55 | 8.561329e-52 |
| MsG0280008407.01 | MsG0580025515.01 | 0.853850 | 1.818874e-61 | 3.984534e-58 |
| MsG0280009752.01 | MsG0580025515.01 | 0.814241 | 1.703377e-51 | 1.136866e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025515.01.T01 | MTR_5g030070 | 98.885 | 269 | 3 | 0 | 1 | 269 | 1 | 269 | 0.0 | 546 |
| MsG0580025515.01.T01 | MTR_8g066790 | 75.093 | 269 | 67 | 0 | 1 | 269 | 1 | 269 | 2.89e-151 | 423 |
| MsG0580025515.01.T01 | MTR_8g101600 | 50.373 | 268 | 133 | 0 | 1 | 268 | 1 | 268 | 8.50e-100 | 293 |
| MsG0580025515.01.T01 | MTR_4g061010 | 45.956 | 272 | 141 | 3 | 1 | 266 | 1 | 272 | 2.20e-78 | 239 |
| MsG0580025515.01.T01 | MTR_4g129670 | 45.318 | 267 | 142 | 3 | 1 | 264 | 1 | 266 | 3.98e-76 | 232 |
| MsG0580025515.01.T01 | MTR_1g095530 | 41.445 | 263 | 144 | 3 | 4 | 266 | 5 | 257 | 2.16e-66 | 208 |
| MsG0580025515.01.T01 | MTR_6g033335 | 41.016 | 256 | 141 | 3 | 4 | 259 | 5 | 250 | 2.35e-66 | 208 |
| MsG0580025515.01.T01 | MTR_4g112570 | 34.231 | 260 | 168 | 2 | 3 | 259 | 6 | 265 | 7.20e-54 | 176 |
| MsG0580025515.01.T01 | MTR_8g097280 | 31.984 | 247 | 159 | 4 | 1 | 244 | 3 | 243 | 7.51e-49 | 163 |
| MsG0580025515.01.T01 | MTR_2g056130 | 31.250 | 256 | 159 | 3 | 3 | 256 | 4 | 244 | 5.16e-44 | 150 |
| MsG0580025515.01.T01 | MTR_5g097460 | 28.458 | 253 | 155 | 6 | 5 | 252 | 50 | 281 | 1.42e-29 | 114 |
| MsG0580025515.01.T01 | MTR_8g097250 | 28.866 | 97 | 64 | 2 | 119 | 212 | 28 | 122 | 7.40e-12 | 61.6 |
| MsG0580025515.01.T01 | MTR_5g097450 | 30.233 | 129 | 66 | 4 | 118 | 241 | 84 | 193 | 1.24e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025515.01.T01 | AT5G46700 | 67.286 | 269 | 88 | 0 | 1 | 269 | 1 | 269 | 2.21e-128 | 365 |
| MsG0580025515.01.T01 | AT2G19580 | 57.249 | 269 | 114 | 1 | 1 | 268 | 1 | 269 | 1.71e-100 | 295 |
| MsG0580025515.01.T01 | AT3G45600 | 44.528 | 265 | 141 | 3 | 1 | 259 | 1 | 265 | 1.81e-81 | 247 |
| MsG0580025515.01.T01 | AT4G28050 | 42.636 | 258 | 146 | 1 | 4 | 259 | 5 | 262 | 4.42e-78 | 237 |
| MsG0580025515.01.T01 | AT4G30430 | 41.603 | 262 | 151 | 1 | 3 | 262 | 4 | 265 | 2.43e-76 | 233 |
| MsG0580025515.01.T01 | AT2G23810 | 42.966 | 263 | 147 | 2 | 4 | 263 | 5 | 267 | 3.22e-76 | 233 |
| MsG0580025515.01.T01 | AT5G60220 | 43.893 | 262 | 141 | 3 | 1 | 256 | 1 | 262 | 2.11e-63 | 202 |
| MsG0580025515.01.T01 | AT1G18520 | 38.550 | 262 | 153 | 4 | 3 | 257 | 4 | 264 | 5.31e-61 | 194 |
| MsG0580025515.01.T01 | AT3G12090 | 40.316 | 253 | 139 | 4 | 4 | 256 | 5 | 245 | 1.33e-58 | 188 |
| MsG0580025515.01.T01 | AT4G23410 | 37.066 | 259 | 152 | 3 | 3 | 261 | 4 | 251 | 3.23e-55 | 179 |
| MsG0580025515.01.T01 | AT1G63260 | 32.171 | 258 | 167 | 3 | 1 | 257 | 3 | 253 | 2.83e-54 | 176 |
| MsG0580025515.01.T01 | AT1G63260 | 32.510 | 243 | 162 | 2 | 1 | 242 | 3 | 244 | 3.47e-51 | 169 |
| MsG0580025515.01.T01 | AT1G63260 | 32.510 | 243 | 162 | 2 | 1 | 242 | 3 | 244 | 3.47e-51 | 169 |
| MsG0580025515.01.T01 | AT1G63260 | 32.510 | 243 | 162 | 2 | 1 | 242 | 3 | 244 | 4.54e-51 | 169 |
| MsG0580025515.01.T01 | AT1G63260 | 32.719 | 217 | 144 | 2 | 1 | 216 | 3 | 218 | 3.58e-46 | 154 |
| MsG0580025515.01.T01 | AT3G45600 | 43.275 | 171 | 92 | 2 | 1 | 166 | 1 | 171 | 3.08e-43 | 146 |
| MsG0580025515.01.T01 | AT2G03840 | 29.707 | 239 | 160 | 4 | 19 | 251 | 32 | 268 | 1.44e-37 | 134 |
| MsG0580025515.01.T01 | AT1G63260 | 30.337 | 178 | 123 | 1 | 1 | 178 | 3 | 179 | 2.28e-33 | 121 |
| MsG0580025515.01.T01 | AT5G23030 | 27.132 | 258 | 183 | 2 | 1 | 256 | 2 | 256 | 2.99e-31 | 117 |
| MsG0580025515.01.T01 | AT5G57810 | 26.316 | 133 | 82 | 1 | 131 | 247 | 173 | 305 | 4.51e-16 | 77.0 |
Find 51 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAACCTAATAGGGCTTATT+TGG | 0.104253 | 5:+19865152 | MsG0580025515.01.T01:CDS |
| AGGGGATTATGAGAGGGTTT+TGG | 0.177264 | 5:-19864813 | None:intergenic |
| TATTATACTTCTTGTTTGTT+TGG | 0.243058 | 5:+19865086 | MsG0580025515.01.T01:CDS |
| ATGTCTGCTGCATTGTTTAT+TGG | 0.243435 | 5:-19865708 | None:intergenic |
| CTGGTCTCATTGGTTCCTTT+TGG | 0.258467 | 5:+19865016 | MsG0580025515.01.T01:CDS |
| TATTCCAAATAAGCCCTATT+AGG | 0.318593 | 5:-19865156 | None:intergenic |
| TTAATTGTGGTTTATTTGAT+TGG | 0.318944 | 5:+19865864 | MsG0580025515.01.T01:CDS |
| CAATCATTGGTGCTGGAATC+TGG | 0.324358 | 5:+19864905 | MsG0580025515.01.T01:CDS |
| ATGGTATGATTGAACCTAAT+AGG | 0.329364 | 5:+19865142 | MsG0580025515.01.T01:CDS |
| GCGAAAGAGATCCTCAGTTT+TGG | 0.353207 | 5:-19865907 | None:intergenic |
| AATCCTAGGTGTTCTTCTCT+TGG | 0.358812 | 5:+19864981 | MsG0580025515.01.T01:CDS |
| GGCTTATTTGGAATACCGTT+TGG | 0.361064 | 5:+19865164 | MsG0580025515.01.T01:CDS |
| CTGGTTTGTTGGCAAATCTT+AGG | 0.375317 | 5:+19865793 | MsG0580025515.01.T01:CDS |
| GTGTCTCTAGCTGGTCTCAT+TGG | 0.382374 | 5:+19865006 | MsG0580025515.01.T01:CDS |
| CTCTTGGTTGTGTCTCTAGC+TGG | 0.384471 | 5:+19864997 | MsG0580025515.01.T01:CDS |
| CATTTGTGAACCCAACATAC+TGG | 0.397625 | 5:+19865679 | MsG0580025515.01.T01:CDS |
| TTGGACTAATCCAGTATGTT+GGG | 0.406980 | 5:-19865689 | None:intergenic |
| CAGATTCCAGCACCAATGAT+TGG | 0.408669 | 5:-19864904 | None:intergenic |
| TTTAACATTGGTGATGAAAT+TGG | 0.419620 | 5:+19865991 | MsG0580025515.01.T01:three_prime_UTR |
| AGTTGTAACAAAGTTGTGTT+TGG | 0.428684 | 5:-19865755 | None:intergenic |
| AGATTCCAGCACCAATGATT+GGG | 0.447069 | 5:-19864903 | None:intergenic |
| TGGTATGATTGAACCTAATA+GGG | 0.447157 | 5:+19865143 | MsG0580025515.01.T01:CDS |
| AACTGTGACTCATGCAAAGC+TGG | 0.452176 | 5:+19865774 | MsG0580025515.01.T01:CDS |
| GCAATGAGCAACAACATCAT+AGG | 0.463723 | 5:+19864847 | MsG0580025515.01.T01:CDS |
| ATTGGACTAATCCAGTATGT+TGG | 0.468680 | 5:-19865690 | None:intergenic |
| GTTTACATGGTTACTCTTCG+TGG | 0.472729 | 5:+19865117 | MsG0580025515.01.T01:CDS |
| ATCCTCTCAATCCCAATCAT+TGG | 0.477309 | 5:+19864892 | MsG0580025515.01.T01:CDS |
| CTCATGCAAAGCTGGTTTGT+TGG | 0.480759 | 5:+19865782 | MsG0580025515.01.T01:CDS |
| TCAATCCCAATCATTGGTGC+TGG | 0.481205 | 5:+19864898 | MsG0580025515.01.T01:CDS |
| ATGGTTACTCTTCGTGGTCA+TGG | 0.481914 | 5:+19865123 | MsG0580025515.01.T01:CDS |
| CAGACATGGATTGTCTTCAA+TGG | 0.495893 | 5:+19865724 | MsG0580025515.01.T01:CDS |
| CAATGGCCAGTCATAATCCT+AGG | 0.501117 | 5:+19864967 | MsG0580025515.01.T01:CDS |
| TTGAGAGGATTACAGCTACT+AGG | 0.501297 | 5:-19864879 | None:intergenic |
| GAGACATGAGATTCTCCAAA+AGG | 0.510546 | 5:-19865031 | None:intergenic |
| TGGCAAATCTTAGGAAGGAA+TGG | 0.515362 | 5:+19865802 | MsG0580025515.01.T01:CDS |
| CACCAATGATTGGGATTGAG+AGG | 0.525563 | 5:-19864894 | None:intergenic |
| AGCATGTAAGATGTAAAGCT+TGG | 0.526590 | 5:+19865965 | MsG0580025515.01.T01:three_prime_UTR |
| GTGTATGCACATTGTGTTGG+TGG | 0.546354 | 5:-19865657 | None:intergenic |
| TGCACGTCTTACACCAATGC+AGG | 0.553446 | 5:+19865320 | MsG0580025515.01.T01:CDS |
| AACTTGACACATGTGTCTGC+TGG | 0.556740 | 5:-19864940 | None:intergenic |
| AATAAACAATGCAGCAGACA+TGG | 0.558636 | 5:+19865710 | MsG0580025515.01.T01:CDS |
| TTTGTTGGCAAATCTTAGGA+AGG | 0.565313 | 5:+19865797 | MsG0580025515.01.T01:CDS |
| CTCTTTCGCAAGTACAAACA+AGG | 0.567497 | 5:+19865921 | MsG0580025515.01.T01:CDS |
| AAAGCTTGGTGCTTTAACAT+TGG | 0.572107 | 5:+19865979 | MsG0580025515.01.T01:three_prime_UTR |
| CTAGGATTATGACTGGCCAT+TGG | 0.581862 | 5:-19864966 | None:intergenic |
| TAAGCACAAGCATAGCAACT+AGG | 0.591892 | 5:-19865065 | None:intergenic |
| AGAACACCTAGGATTATGAC+TGG | 0.617019 | 5:-19864973 | None:intergenic |
| CATGTGTCAAGTTTCTCCAA+TGG | 0.625370 | 5:+19864950 | MsG0580025515.01.T01:CDS |
| AATGTGTATGCACATTGTGT+TGG | 0.656084 | 5:-19865660 | None:intergenic |
| CAACCAAGAGAAGAACACCT+AGG | 0.688813 | 5:-19864984 | None:intergenic |
| CTTAGTGAAACAAATATGTG+TGG | 0.704201 | 5:+19865255 | MsG0580025515.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAGAGGAAAAAAATTAT+TGG | - | Chr5:19865611-19865630 | None:intergenic | 15.0% |
| !! | AGACTGAAAAATAATTAAAG+AGG | - | Chr5:19865625-19865644 | None:intergenic | 20.0% |
| !! | TTAATTGTGGTTTATTTGAT+TGG | + | Chr5:19865864-19865883 | MsG0580025515.01.T01:CDS | 20.0% |
| !!! | CTTTAATTATTTTTCAGTCT+GGG | + | Chr5:19865625-19865644 | MsG0580025515.01.T01:intron | 20.0% |
| !!! | TATTATACTTCTTGTTTGTT+TGG | + | Chr5:19865086-19865105 | MsG0580025515.01.T01:CDS | 20.0% |
| !!! | TCTTTAATTATTTTTCAGTC+TGG | + | Chr5:19865624-19865643 | MsG0580025515.01.T01:intron | 20.0% |
| !!! | TTTGGTTATTTTTGTTTACA+TGG | + | Chr5:19865104-19865123 | MsG0580025515.01.T01:CDS | 20.0% |
| ! | AACTAAAAAAATACCTGCAT+TGG | - | Chr5:19865336-19865355 | None:intergenic | 25.0% |
| !!! | AGTTAGAAGTTCTTTTAAGT+GGG | + | Chr5:19865215-19865234 | MsG0580025515.01.T01:CDS | 25.0% |
| !!! | TTTAACATTGGTGATGAAAT+TGG | + | Chr5:19865991-19866010 | MsG0580025515.01.T01:three_prime_UTR | 25.0% |
| AGTTGTAACAAAGTTGTGTT+TGG | - | Chr5:19865758-19865777 | None:intergenic | 30.0% | |
| ATGGTATGATTGAACCTAAT+AGG | + | Chr5:19865142-19865161 | MsG0580025515.01.T01:CDS | 30.0% | |
| CTTAGTGAAACAAATATGTG+TGG | + | Chr5:19865255-19865274 | MsG0580025515.01.T01:CDS | 30.0% | |
| TATTCCAAATAAGCCCTATT+AGG | - | Chr5:19865159-19865178 | None:intergenic | 30.0% | |
| TGGTATGATTGAACCTAATA+GGG | + | Chr5:19865143-19865162 | MsG0580025515.01.T01:CDS | 30.0% | |
| ! | GAGTTAGAAGTTCTTTTAAG+TGG | + | Chr5:19865214-19865233 | MsG0580025515.01.T01:CDS | 30.0% |
| ! | GTTGAATCAAAGTTTTCGTA+TGG | + | Chr5:19865281-19865300 | MsG0580025515.01.T01:CDS | 30.0% |
| !!! | CAGTGTTGTTGTTTTAATTG+TGG | + | Chr5:19865851-19865870 | MsG0580025515.01.T01:CDS | 30.0% |
| AATAAACAATGCAGCAGACA+TGG | + | Chr5:19865710-19865729 | MsG0580025515.01.T01:CDS | 35.0% | |
| AATGTGTATGCACATTGTGT+TGG | - | Chr5:19865663-19865682 | None:intergenic | 35.0% | |
| ACCCAGAAAAATCATCCAAA+CGG | - | Chr5:19865182-19865201 | None:intergenic | 35.0% | |
| AGCATGTAAGATGTAAAGCT+TGG | + | Chr5:19865965-19865984 | MsG0580025515.01.T01:three_prime_UTR | 35.0% | |
| ATGTCTGCTGCATTGTTTAT+TGG | - | Chr5:19865711-19865730 | None:intergenic | 35.0% | |
| ATTGGACTAATCCAGTATGT+TGG | - | Chr5:19865693-19865712 | None:intergenic | 35.0% | |
| CATAATCCCCTCCAAAAAAA+TGG | + | Chr5:19864825-19864844 | MsG0580025515.01.T01:exon | 35.0% | |
| TGAACCTAATAGGGCTTATT+TGG | + | Chr5:19865152-19865171 | MsG0580025515.01.T01:CDS | 35.0% | |
| TTGGACTAATCCAGTATGTT+GGG | - | Chr5:19865692-19865711 | None:intergenic | 35.0% | |
| TTTGTTGGCAAATCTTAGGA+AGG | + | Chr5:19865797-19865816 | MsG0580025515.01.T01:CDS | 35.0% | |
| ! | ACCGTTTGGATGATTTTTCT+GGG | + | Chr5:19865178-19865197 | MsG0580025515.01.T01:CDS | 35.0% |
| ! | TACCGTTTGGATGATTTTTC+TGG | + | Chr5:19865177-19865196 | MsG0580025515.01.T01:CDS | 35.0% |
| !! | AAAGCTTGGTGCTTTAACAT+TGG | + | Chr5:19865979-19865998 | MsG0580025515.01.T01:three_prime_UTR | 35.0% |
| !! | GATCTGTTTCTAGTTTCACT+CGG | - | Chr5:19865392-19865411 | None:intergenic | 35.0% |
| !! | TGATTGGTTGCTTTGCTTTT+AGG | + | Chr5:19865880-19865899 | MsG0580025515.01.T01:CDS | 35.0% |
| !! | TTTTAGGAATGCCAAAACTG+AGG | + | Chr5:19865896-19865915 | MsG0580025515.01.T01:CDS | 35.0% |
| !!! | CTCATTGCCATTTTTTTGGA+GGG | - | Chr5:19864835-19864854 | None:intergenic | 35.0% |
| !!! | GTGTGGATTTTGTTGTTTGT+TGG | - | Chr5:19864782-19864801 | None:intergenic | 35.0% |
| !!! | GTTGCTCATTGCCATTTTTT+TGG | - | Chr5:19864839-19864858 | None:intergenic | 35.0% |
| !!! | TCATTGCCATTTTTTTGGAG+GGG | - | Chr5:19864834-19864853 | None:intergenic | 35.0% |
| AATCCTAGGTGTTCTTCTCT+TGG | + | Chr5:19864981-19865000 | MsG0580025515.01.T01:CDS | 40.0% | |
| AGAACACCTAGGATTATGAC+TGG | - | Chr5:19864976-19864995 | None:intergenic | 40.0% | |
| AGATTCCAGCACCAATGATT+GGG | - | Chr5:19864906-19864925 | None:intergenic | 40.0% | |
| ATCCTCTCAATCCCAATCAT+TGG | + | Chr5:19864892-19864911 | MsG0580025515.01.T01:CDS | 40.0% | |
| CATGTGTCAAGTTTCTCCAA+TGG | + | Chr5:19864950-19864969 | MsG0580025515.01.T01:CDS | 40.0% | |
| CATTTGTGAACCCAACATAC+TGG | + | Chr5:19865679-19865698 | MsG0580025515.01.T01:CDS | 40.0% | |
| CTCAAGATAAACCCACTTCT+GGG | + | Chr5:19865424-19865443 | MsG0580025515.01.T01:intron | 40.0% | |
| CTCTTTCGCAAGTACAAACA+AGG | + | Chr5:19865921-19865940 | MsG0580025515.01.T01:CDS | 40.0% | |
| GAGACATGAGATTCTCCAAA+AGG | - | Chr5:19865034-19865053 | None:intergenic | 40.0% | |
| GCAATGAGCAACAACATCAT+AGG | + | Chr5:19864847-19864866 | MsG0580025515.01.T01:CDS | 40.0% | |
| GTTTACATGGTTACTCTTCG+TGG | + | Chr5:19865117-19865136 | MsG0580025515.01.T01:CDS | 40.0% | |
| TAAGCACAAGCATAGCAACT+AGG | - | Chr5:19865068-19865087 | None:intergenic | 40.0% | |
| TCTCAAGATAAACCCACTTC+TGG | + | Chr5:19865423-19865442 | MsG0580025515.01.T01:intron | 40.0% | |
| TGGCAAATCTTAGGAAGGAA+TGG | + | Chr5:19865802-19865821 | MsG0580025515.01.T01:CDS | 40.0% | |
| TTGAGAGGATTACAGCTACT+AGG | - | Chr5:19864882-19864901 | None:intergenic | 40.0% | |
| ! | CAGACATGGATTGTCTTCAA+TGG | + | Chr5:19865724-19865743 | MsG0580025515.01.T01:CDS | 40.0% |
| ! | GGCTTATTTGGAATACCGTT+TGG | + | Chr5:19865164-19865183 | MsG0580025515.01.T01:CDS | 40.0% |
| ! | TCAAAGTTTTCGTATGGCTC+AGG | + | Chr5:19865287-19865306 | MsG0580025515.01.T01:CDS | 40.0% |
| !! | CTGGTTTGTTGGCAAATCTT+AGG | + | Chr5:19865793-19865812 | MsG0580025515.01.T01:CDS | 40.0% |
| !! | TTTTGGAGGGGATTATGAGA+GGG | - | Chr5:19864822-19864841 | None:intergenic | 40.0% |
| !! | TTTTTGGAGGGGATTATGAG+AGG | - | Chr5:19864823-19864842 | None:intergenic | 40.0% |
| !!! | GCTCATTGCCATTTTTTTGG+AGG | - | Chr5:19864836-19864855 | None:intergenic | 40.0% |
| AACTGTGACTCATGCAAAGC+TGG | + | Chr5:19865774-19865793 | MsG0580025515.01.T01:CDS | 45.0% | |
| AACTTGACACATGTGTCTGC+TGG | - | Chr5:19864943-19864962 | None:intergenic | 45.0% | |
| ATGGTTACTCTTCGTGGTCA+TGG | + | Chr5:19865123-19865142 | MsG0580025515.01.T01:CDS | 45.0% | |
| CAACCAAGAGAAGAACACCT+AGG | - | Chr5:19864987-19865006 | None:intergenic | 45.0% | |
| CAATGGCCAGTCATAATCCT+AGG | + | Chr5:19864967-19864986 | MsG0580025515.01.T01:CDS | 45.0% | |
| CACCAATGATTGGGATTGAG+AGG | - | Chr5:19864897-19864916 | None:intergenic | 45.0% | |
| CAGATTCCAGCACCAATGAT+TGG | - | Chr5:19864907-19864926 | None:intergenic | 45.0% | |
| GTGCTTCTCTTCAAGGTCTT+AGG | + | Chr5:19865457-19865476 | MsG0580025515.01.T01:intron | 45.0% | |
| GTGTATGCACATTGTGTTGG+TGG | - | Chr5:19865660-19865679 | None:intergenic | 45.0% | |
| TCAATCCCAATCATTGGTGC+TGG | + | Chr5:19864898-19864917 | MsG0580025515.01.T01:CDS | 45.0% | |
| ! | AGGGGATTATGAGAGGGTTT+TGG | - | Chr5:19864816-19864835 | None:intergenic | 45.0% |
| ! | CTAGGATTATGACTGGCCAT+TGG | - | Chr5:19864969-19864988 | None:intergenic | 45.0% |
| ! | GCGAAAGAGATCCTCAGTTT+TGG | - | Chr5:19865910-19865929 | None:intergenic | 45.0% |
| ! | GGGGATTATGAGAGGGTTTT+GGG | - | Chr5:19864815-19864834 | None:intergenic | 45.0% |
| ! | TGAGAGTGTGCTTCTCTTCA+AGG | + | Chr5:19865450-19865469 | MsG0580025515.01.T01:intron | 45.0% |
| !! | CAATCATTGGTGCTGGAATC+TGG | + | Chr5:19864905-19864924 | MsG0580025515.01.T01:CDS | 45.0% |
| !! | CTCATGCAAAGCTGGTTTGT+TGG | + | Chr5:19865782-19865801 | MsG0580025515.01.T01:CDS | 45.0% |
| !!! | AGAGGGTTTTGGGTTTGAGA+TGG | - | Chr5:19864805-19864824 | None:intergenic | 45.0% |
| !!! | CTGGTCTCATTGGTTCCTTT+TGG | + | Chr5:19865016-19865035 | MsG0580025515.01.T01:CDS | 45.0% |
| !!! | GAGGGTTTTGGGTTTGAGAT+GGG | - | Chr5:19864804-19864823 | None:intergenic | 45.0% |
| !!! | TTTTGGGTTTGAGATGGGTG+TGG | - | Chr5:19864799-19864818 | None:intergenic | 45.0% |
| ACACTCTCAGATCCCAGAAG+TGG | - | Chr5:19865439-19865458 | None:intergenic | 50.0% | |
| CACTCTCAGATCCCAGAAGT+GGG | - | Chr5:19865438-19865457 | None:intergenic | 50.0% | |
| CTCTTGGTTGTGTCTCTAGC+TGG | + | Chr5:19864997-19865016 | MsG0580025515.01.T01:CDS | 50.0% | |
| TGCACGTCTTACACCAATGC+AGG | + | Chr5:19865320-19865339 | MsG0580025515.01.T01:CDS | 50.0% | |
| ! | GTGTCTCTAGCTGGTCTCAT+TGG | + | Chr5:19865006-19865025 | MsG0580025515.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 19864770 | 19866019 | 19864770 | ID=MsG0580025515.01;Name=MsG0580025515.01 |
| Chr5 | mRNA | 19864770 | 19866019 | 19864770 | ID=MsG0580025515.01.T01;Parent=MsG0580025515.01;Name=MsG0580025515.01.T01;_AED=0.49;_eAED=0.49;_QI=74|1|1|1|1|1|2|66|269 |
| Chr5 | exon | 19864770 | 19865341 | 19864770 | ID=MsG0580025515.01.T01:exon:8385;Parent=MsG0580025515.01.T01 |
| Chr5 | exon | 19865642 | 19866019 | 19865642 | ID=MsG0580025515.01.T01:exon:8386;Parent=MsG0580025515.01.T01 |
| Chr5 | five_prime_UTR | 19864770 | 19864843 | 19864770 | ID=MsG0580025515.01.T01:five_prime_utr;Parent=MsG0580025515.01.T01 |
| Chr5 | CDS | 19864844 | 19865341 | 19864844 | ID=MsG0580025515.01.T01:cds;Parent=MsG0580025515.01.T01 |
| Chr5 | CDS | 19865642 | 19865953 | 19865642 | ID=MsG0580025515.01.T01:cds;Parent=MsG0580025515.01.T01 |
| Chr5 | three_prime_UTR | 19865954 | 19866019 | 19865954 | ID=MsG0580025515.01.T01:three_prime_utr;Parent=MsG0580025515.01.T01 |
| Gene Sequence |
| Protein sequence |
