Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019880.01.T01 | XP_013443645.1 | 89.655 | 319 | 28 | 2 | 1 | 319 | 1 | 314 | 0 | 543 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019880.01.T01 | A0A072TVX0 | 89.655 | 319 | 28 | 2 | 1 | 319 | 1 | 314 | 0.0 | 543 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048410.01 | MsG0480019880.01 | 0.823030 | 1.725222e-53 | 1.468530e-50 |
| MsG0180000019.01 | MsG0480019880.01 | 0.804233 | 2.383584e-49 | 1.222456e-46 |
| MsG0180000089.01 | MsG0480019880.01 | 0.863202 | 2.944599e-64 | 8.854199e-61 |
| MsG0180000222.01 | MsG0480019880.01 | 0.850883 | 1.273290e-60 | 2.530713e-57 |
| MsG0180001053.01 | MsG0480019880.01 | 0.853609 | 2.134323e-61 | 4.638359e-58 |
| MsG0180002912.01 | MsG0480019880.01 | 0.823890 | 1.085855e-53 | 9.470167e-51 |
| MsG0180003241.01 | MsG0480019880.01 | 0.816409 | 5.612628e-52 | 3.973796e-49 |
| MsG0180004110.01 | MsG0480019880.01 | 0.852134 | 5.634685e-61 | 1.166039e-57 |
| MsG0180004752.01 | MsG0480019880.01 | 0.878366 | 2.977381e-69 | 1.550421e-65 |
| MsG0180004762.01 | MsG0480019880.01 | 0.803965 | 2.709645e-49 | 1.380265e-46 |
| MsG0180004867.01 | MsG0480019880.01 | 0.839458 | 1.565773e-57 | 2.163638e-54 |
| MsG0180005172.01 | MsG0480019880.01 | 0.847514 | 1.102088e-59 | 1.963751e-56 |
| MsG0180005331.01 | MsG0480019880.01 | 0.864618 | 1.068080e-64 | 3.375215e-61 |
| MsG0180005813.01 | MsG0480019880.01 | 0.815874 | 7.392066e-52 | 5.157464e-49 |
| MsG0180006012.01 | MsG0480019880.01 | 0.881267 | 2.766721e-70 | 1.610036e-66 |
| MsG0180006016.01 | MsG0480019880.01 | 0.816444 | 5.511541e-52 | 3.905825e-49 |
| MsG0180006047.01 | MsG0480019880.01 | 0.850393 | 1.747986e-60 | 3.419081e-57 |
| MsG0280011053.01 | MsG0480019880.01 | 0.832990 | 6.896787e-56 | 7.837143e-53 |
| MsG0280011152.01 | MsG0480019880.01 | 0.848163 | 7.303171e-60 | 1.328201e-56 |
| MsG0280011288.01 | MsG0480019880.01 | 0.810093 | 1.368229e-50 | 8.172961e-48 |
| MsG0280011473.01 | MsG0480019880.01 | 0.826901 | 2.102956e-54 | 1.998699e-51 |
| MsG0380014920.01 | MsG0480019880.01 | 0.832235 | 1.061321e-55 | 1.179052e-52 |
| MsG0480018500.01 | MsG0480019880.01 | 0.832484 | 9.206342e-56 | 1.030428e-52 |
| MsG0480018998.01 | MsG0480019880.01 | 0.839976 | 1.147790e-57 | 1.611898e-54 |
| MsG0480019038.01 | MsG0480019880.01 | 0.800601 | 1.335074e-48 | 6.242269e-46 |
| MsG0480019295.01 | MsG0480019880.01 | 0.803428 | 3.502669e-49 | 1.759723e-46 |
| MsG0480019880.01 | MsG0480020444.01 | 0.877883 | 4.396407e-69 | 2.247876e-65 |
| MsG0480019880.01 | MsG0480020445.01 | 0.831011 | 2.126087e-55 | 2.278143e-52 |
| MsG0480019880.01 | MsG0480021134.01 | 0.810681 | 1.021617e-50 | 6.198226e-48 |
| MsG0480019880.01 | MsG0480021298.01 | 0.804965 | 1.677141e-49 | 8.766068e-47 |
| MsG0480019880.01 | MsG0480021642.01 | 0.862701 | 4.205275e-64 | 1.242837e-60 |
| MsG0480019880.01 | MsG0480022125.01 | 0.801148 | 1.032428e-48 | 4.894648e-46 |
| MsG0480019880.01 | MsG0480022152.01 | 0.818671 | 1.735416e-52 | 1.307412e-49 |
| MsG0480019880.01 | MsG0480022274.01 | 0.811162 | 8.036406e-51 | 4.938385e-48 |
| MsG0480019880.01 | MsG0480022479.01 | 0.839029 | 2.022850e-57 | 2.758659e-54 |
| MsG0480019880.01 | MsG0480022480.01 | 0.805269 | 1.448509e-49 | 7.629893e-47 |
| MsG0480019880.01 | MsG0480022486.01 | 0.832402 | 9.651315e-56 | 1.077606e-52 |
| MsG0480019880.01 | MsG0480022760.01 | 0.810365 | 1.194959e-50 | 7.189241e-48 |
| MsG0480019880.01 | MsG0480023318.01 | 0.863089 | 3.192373e-64 | 9.561598e-61 |
| MsG0480019880.01 | MsG0480023517.01 | 0.816507 | 5.336119e-52 | 3.788113e-49 |
| MsG0480019880.01 | MsG0480023947.01 | 0.819814 | 9.533585e-53 | 7.413984e-50 |
| MsG0480019880.01 | MsG0580024407.01 | 0.852539 | 4.321266e-61 | 9.058273e-58 |
| MsG0480019880.01 | MsG0580024871.01 | 0.845535 | 3.823161e-59 | 6.391028e-56 |
| MsG0480019880.01 | MsG0580024999.01 | 0.844998 | 5.339713e-59 | 8.774338e-56 |
| MsG0480019880.01 | MsG0580025143.01 | 0.806980 | 6.318919e-50 | 3.477912e-47 |
| MsG0480019880.01 | MsG0580025338.01 | 0.838951 | 2.118862e-57 | 2.882657e-54 |
| MsG0480019880.01 | MsG0580025860.01 | 0.825112 | 5.597194e-54 | 5.054840e-51 |
| MsG0480019880.01 | MsG0580027073.01 | 0.818609 | 1.793174e-52 | 1.348521e-49 |
| MsG0480019880.01 | MsG0580027129.01 | 0.827953 | 1.176858e-54 | 1.153346e-51 |
| MsG0480019880.01 | MsG0580027227.01 | 0.813866 | 2.060444e-51 | 1.361251e-48 |
| MsG0480019880.01 | MsG0580027345.01 | 0.836331 | 9.961068e-57 | 1.251441e-53 |
| MsG0480019880.01 | MsG0580027847.01 | 0.845697 | 3.454300e-59 | 5.805495e-56 |
| MsG0480019880.01 | MsG0580028355.01 | 0.868915 | 4.576867e-66 | 1.684723e-62 |
| MsG0480019880.01 | MsG0580028416.01 | 0.816281 | 5.994499e-52 | 4.229694e-49 |
| MsG0480019880.01 | MsG0580028560.01 | 0.861175 | 1.233566e-63 | 3.458921e-60 |
| MsG0480019880.01 | MsG0580029798.01 | 0.840308 | 9.401903e-58 | 1.333738e-54 |
| MsG0480019880.01 | MsG0580029901.01 | 0.850812 | 1.332875e-60 | 2.643252e-57 |
| MsG0480019880.01 | MsG0580029906.01 | 0.854432 | 1.235608e-61 | 2.758929e-58 |
| MsG0480019880.01 | MsG0580029930.01 | 0.841518 | 4.525985e-58 | 6.667134e-55 |
| MsG0480019880.01 | MsG0680035741.01 | 0.801100 | 1.055790e-48 | 4.999630e-46 |
| MsG0480019880.01 | MsG0780036514.01 | 0.806542 | 7.821504e-50 | 4.256666e-47 |
| MsG0480019880.01 | MsG0780037109.01 | 0.829679 | 4.496630e-55 | 4.635094e-52 |
| MsG0480019880.01 | MsG0780037258.01 | 0.802433 | 5.623231e-49 | 2.754280e-46 |
| MsG0480019880.01 | MsG0780037599.01 | 0.857293 | 1.801439e-62 | 4.424103e-59 |
| MsG0480019880.01 | MsG0780038123.01 | 0.838915 | 2.164714e-57 | 2.941770e-54 |
| MsG0480019880.01 | MsG0780038135.01 | 0.865153 | 7.260471e-65 | 2.338409e-61 |
| MsG0480019880.01 | MsG0780038444.01 | 0.817426 | 3.317774e-52 | 2.415490e-49 |
| MsG0480019880.01 | MsG0780038515.01 | 0.835854 | 1.317079e-56 | 1.630721e-53 |
| MsG0480019880.01 | MsG0780038921.01 | 0.826670 | 2.388506e-54 | 2.254880e-51 |
| MsG0480019880.01 | MsG0780038925.01 | 0.836394 | 9.601544e-57 | 1.208432e-53 |
| MsG0480019880.01 | MsG0780039450.01 | 0.856486 | 3.113553e-62 | 7.444341e-59 |
| MsG0480019880.01 | MsG0780039472.01 | 0.815714 | 8.024710e-52 | 5.574889e-49 |
| MsG0480019880.01 | MsG0780039877.01 | 0.832846 | 7.485369e-56 | 8.468719e-53 |
| MsG0480019880.01 | MsG0780040144.01 | 0.877347 | 6.758720e-69 | 3.387569e-65 |
| MsG0480019880.01 | MsG0780040442.01 | 0.814701 | 1.347108e-51 | 9.103543e-49 |
| MsG0480019880.01 | MsG0780040505.01 | 0.819692 | 1.016300e-52 | 7.876191e-50 |
| MsG0480019880.01 | MsG0780041357.01 | 0.827107 | 1.877953e-54 | 1.795456e-51 |
| MsG0480019880.01 | MsG0780041380.01 | 0.827390 | 1.606082e-54 | 1.548588e-51 |
| MsG0480019880.01 | MsG0780041589.01 | 0.802141 | 6.458790e-49 | 3.139939e-46 |
| MsG0480019880.01 | MsG0880041985.01 | 0.857925 | 1.170376e-62 | 2.936371e-59 |
| MsG0480019880.01 | MsG0880042368.01 | 0.852604 | 4.141071e-61 | 8.699161e-58 |
| MsG0480019880.01 | MsG0880042589.01 | 0.866034 | 3.829745e-65 | 1.272465e-61 |
| MsG0480019880.01 | MsG0880042843.01 | 0.866584 | 2.561532e-65 | 8.678204e-62 |
| MsG0480019880.01 | MsG0880042967.01 | 0.840775 | 7.095182e-58 | 1.021309e-54 |
| MsG0480019880.01 | MsG0880043001.01 | 0.800320 | 1.522997e-48 | 7.070494e-46 |
| MsG0480019880.01 | MsG0880043094.01 | 0.811147 | 8.097502e-51 | 4.974008e-48 |
| MsG0480019880.01 | MsG0880043285.01 | 0.869015 | 4.249669e-66 | 1.570038e-62 |
| MsG0480019880.01 | MsG0880043286.01 | 0.807483 | 4.943734e-50 | 2.756890e-47 |
| MsG0480019880.01 | MsG0880043685.01 | 0.847947 | 8.374155e-60 | 1.512747e-56 |
| MsG0480019880.01 | MsG0880043715.01 | 0.865653 | 5.050668e-65 | 1.656159e-61 |
| MsG0480019880.01 | MsG0880043745.01 | 0.801760 | 7.734550e-49 | 3.723756e-46 |
| MsG0480019880.01 | MsG0880044100.01 | 0.859056 | 5.385900e-63 | 1.403668e-59 |
| MsG0480019880.01 | MsG0880044669.01 | 0.802921 | 4.460578e-49 | 2.212051e-46 |
| MsG0480019880.01 | MsG0880045290.01 | 0.821593 | 3.717771e-53 | 3.037675e-50 |
| MsG0480019880.01 | MsG0880045819.01 | 0.824238 | 8.994535e-54 | 7.921458e-51 |
| MsG0480019880.01 | MsG0880046226.01 | 0.855189 | 7.453714e-62 | 1.706440e-58 |
| MsG0480019880.01 | MsG0880046251.01 | -0.829559 | 4.809976e-55 | 4.940067e-52 |
| MsG0480019880.01 | MsG0880046329.01 | 0.867061 | 1.805488e-65 | 6.219635e-62 |
| MsG0480019880.01 | MsG0880046467.01 | 0.816204 | 6.238090e-52 | 4.392189e-49 |
| MsG0480019880.01 | MsG0880046586.01 | 0.834823 | 2.398268e-56 | 2.879054e-53 |
| MsG0480019880.01 | MsG0880046642.01 | 0.851423 | 8.963376e-61 | 1.813475e-57 |
| MsG0480019880.01 | MsG0880047081.01 | 0.804291 | 2.318476e-49 | 1.190904e-46 |
| MsG0480019880.01 | MsG0880047233.01 | 0.842529 | 2.445873e-58 | 3.719274e-55 |
| MsG0480019880.01 | MsG0880047497.01 | 0.826587 | 2.500376e-54 | 2.354677e-51 |
| MsG0380015245.01 | MsG0480019880.01 | 0.821400 | 4.120323e-53 | 3.348526e-50 |
| MsG0380015931.01 | MsG0480019880.01 | 0.817294 | 3.553169e-52 | 2.577312e-49 |
| MsG0380016273.01 | MsG0480019880.01 | 0.805608 | 1.229590e-49 | 6.533613e-47 |
| MsG0380016427.01 | MsG0480019880.01 | 0.805243 | 1.466843e-49 | 7.721375e-47 |
| MsG0380017101.01 | MsG0480019880.01 | 0.812140 | 4.923859e-51 | 3.105679e-48 |
| MsG0380017158.01 | MsG0480019880.01 | 0.853557 | 2.208595e-61 | 4.791254e-58 |
| MsG0380017403.01 | MsG0480019880.01 | 0.801114 | 1.048761e-48 | 4.967943e-46 |
| MsG0280006974.01 | MsG0480019880.01 | 0.823472 | 1.359620e-53 | 1.171792e-50 |
| MsG0280007253.01 | MsG0480019880.01 | 0.803939 | 2.743336e-49 | 1.396570e-46 |
| MsG0280007272.01 | MsG0480019880.01 | 0.871690 | 5.638642e-67 | 2.295447e-63 |
| MsG0280007351.01 | MsG0480019880.01 | 0.816496 | 5.366363e-52 | 3.808366e-49 |
| MsG0280007369.01 | MsG0480019880.01 | 0.866735 | 2.293459e-65 | 7.812149e-62 |
| MsG0280007391.01 | MsG0480019880.01 | 0.879259 | 1.441595e-69 | 7.765736e-66 |
| MsG0280007549.01 | MsG0480019880.01 | 0.806528 | 7.875864e-50 | 4.284552e-47 |
| MsG0280007751.01 | MsG0480019880.01 | 0.806520 | 7.905160e-50 | 4.299691e-47 |
| MsG0280007840.01 | MsG0480019880.01 | 0.826780 | 2.248721e-54 | 2.129485e-51 |
| MsG0280009394.01 | MsG0480019880.01 | 0.801918 | 7.177094e-49 | 3.469429e-46 |
| MsG0280009610.01 | MsG0480019880.01 | 0.800733 | 1.254815e-48 | 5.886597e-46 |
| MsG0280009838.01 | MsG0480019880.01 | 0.818794 | 1.627520e-52 | 1.230272e-49 |
| MsG0280009899.01 | MsG0480019880.01 | 0.821662 | 3.584683e-53 | 2.934697e-50 |
| MsG0280010416.01 | MsG0480019880.01 | 0.803667 | 3.125408e-49 | 1.579788e-46 |
| MsG0280010459.01 | MsG0480019880.01 | 0.829118 | 6.156305e-55 | 6.241761e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019880.01.T01 | MTR_0003s0580 | 89.655 | 319 | 28 | 2 | 1 | 319 | 1 | 314 | 0.0 | 543 |
| MsG0480019880.01.T01 | MTR_3g028060 | 51.701 | 294 | 138 | 4 | 25 | 316 | 15 | 306 | 8.18e-81 | 248 |
| MsG0480019880.01.T01 | MTR_3g034370 | 50.220 | 227 | 111 | 2 | 90 | 316 | 48 | 272 | 1.03e-69 | 218 |
| MsG0480019880.01.T01 | MTR_3g034410 | 42.612 | 291 | 121 | 7 | 25 | 313 | 15 | 261 | 1.27e-54 | 179 |
| MsG0480019880.01.T01 | MTR_3g034540 | 44.628 | 121 | 66 | 1 | 196 | 316 | 1 | 120 | 6.03e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGAAATCCTATCCTTATTT+TGG | 0.090774 | 4:-28898906 | MsG0480019880.01.T01:CDS |
| AAATGAAGATAATTGGTTTC+TGG | 0.148063 | 4:-28898510 | MsG0480019880.01.T01:CDS |
| AGTTGTTTGAAAACAAAATT+TGG | 0.202695 | 4:-28898610 | MsG0480019880.01.T01:CDS |
| TAGATTATCCAATGAGTAAA+TGG | 0.228369 | 4:-28898819 | MsG0480019880.01.T01:CDS |
| TCCAGCTTGTTGTAGAGTTT+TGG | 0.249224 | 4:+28899066 | None:intergenic |
| GATCAATCAACTCAGTTCTA+AGG | 0.257308 | 4:+28898176 | None:intergenic |
| GAGGTTAAGAAAATGGTAAA+TGG | 0.279558 | 4:-28898436 | MsG0480019880.01.T01:CDS |
| TGTGAAGATTAATAGGTTAT+AGG | 0.282501 | 4:-28898111 | MsG0480019880.01.T01:three_prime_UTR |
| AATGAAGATAATTGGTTTCT+GGG | 0.291710 | 4:-28898509 | MsG0480019880.01.T01:CDS |
| CTGATGCTATTTCGTTAATC+AGG | 0.307616 | 4:+28898944 | None:intergenic |
| GTGAATGAAATGAAGATAAT+TGG | 0.309380 | 4:-28898517 | MsG0480019880.01.T01:CDS |
| TTATCCATGGTAATACTCTT+AGG | 0.320589 | 4:-28899099 | None:intergenic |
| ATATATTGAGGTTAAGAAAA+TGG | 0.326568 | 4:-28898443 | MsG0480019880.01.T01:CDS |
| AAATGATGCAGCAAAGGAAT+TGG | 0.358292 | 4:-28898278 | MsG0480019880.01.T01:CDS |
| AAAATGGTAAATGGGTTTGA+TGG | 0.372096 | 4:-28898427 | MsG0480019880.01.T01:CDS |
| AGCAAAGGAATTGGAGATAA+AGG | 0.392312 | 4:-28898269 | MsG0480019880.01.T01:CDS |
| ATAATTGGTTTCTGGGTTTG+TGG | 0.398395 | 4:-28898502 | MsG0480019880.01.T01:CDS |
| TCAACCTCCCATTTACTCAT+TGG | 0.398793 | 4:+28898811 | None:intergenic |
| TTCGGCTCGGAAAGCCTTCA+AGG | 0.403414 | 4:+28898975 | None:intergenic |
| TGTAAAAGCCTTGTTGATCA+AGG | 0.403671 | 4:+28898858 | None:intergenic |
| AGGTTAAGAAAATGGTAAAT+GGG | 0.412672 | 4:-28898435 | MsG0480019880.01.T01:CDS |
| GATGATGATGGTTGTGGTGG+TGG | 0.412788 | 4:+28899034 | None:intergenic |
| GTTAATCAGGTTCGAAACTT+CGG | 0.433919 | 4:+28898957 | None:intergenic |
| TGAGTTATCAGCATCCTTGA+AGG | 0.440044 | 4:-28898989 | MsG0480019880.01.T01:CDS |
| GTTTGTGGAATCTTATTGTC+TGG | 0.447627 | 4:-28898487 | MsG0480019880.01.T01:CDS |
| CTGATAACTCATGAGATTCA+TGG | 0.455666 | 4:+28899001 | None:intergenic |
| CTGAGTTGATTGATCAACTA+AGG | 0.462243 | 4:-28898168 | MsG0480019880.01.T01:CDS |
| AAGGATAGGATTTCCGATCC+AGG | 0.474535 | 4:+28898913 | None:intergenic |
| AAGTTTGTGAAGGTATTTGA+TGG | 0.483946 | 4:-28898241 | MsG0480019880.01.T01:CDS |
| TTTGAATCCAAAATAAGGAT+AGG | 0.490482 | 4:+28898899 | None:intergenic |
| ACTGATCTTGAAGGAGGTAA+AGG | 0.496235 | 4:-28898350 | MsG0480019880.01.T01:CDS |
| GTAGTTGAGATACTCTTCAA+TGG | 0.502635 | 4:+28898786 | None:intergenic |
| AGATTATCCAATGAGTAAAT+GGG | 0.511538 | 4:-28898818 | MsG0480019880.01.T01:CDS |
| GTAAAAGCCTTGTTGATCAA+GGG | 0.513853 | 4:+28898859 | None:intergenic |
| TGATGTTAAGCCATATATTG+AGG | 0.527762 | 4:-28898455 | MsG0480019880.01.T01:CDS |
| ATAGCATCAGATTCGAAACC+TGG | 0.542074 | 4:-28898931 | MsG0480019880.01.T01:CDS |
| ATTGATTCTAAAATCAGTGA+AGG | 0.542847 | 4:-28898388 | MsG0480019880.01.T01:CDS |
| TCCAAAACTCTACAACAAGC+TGG | 0.546418 | 4:-28899067 | MsG0480019880.01.T01:CDS |
| ATCAATCAACTCAGTTCTAA+GGG | 0.562320 | 4:+28898177 | None:intergenic |
| TTGATTCTAAAATCAGTGAA+GGG | 0.585790 | 4:-28898387 | MsG0480019880.01.T01:CDS |
| GATCATGATGATGATGGTTG+TGG | 0.587159 | 4:+28899028 | None:intergenic |
| CATGATGATGATGGTTGTGG+TGG | 0.604612 | 4:+28899031 | None:intergenic |
| ATCAGATTCGAAACCTGGAT+CGG | 0.606501 | 4:-28898926 | MsG0480019880.01.T01:CDS |
| AAAGGTGAACAAGTTTGTGA+AGG | 0.611343 | 4:-28898251 | MsG0480019880.01.T01:CDS |
| ATTTGATGGTGTAAAAGCAG+AGG | 0.615999 | 4:-28898227 | MsG0480019880.01.T01:CDS |
| GGTGATGATCATGATGATGA+TGG | 0.625791 | 4:+28899022 | None:intergenic |
| AATCTTACCCTTGATCAACA+AGG | 0.636503 | 4:-28898866 | MsG0480019880.01.T01:CDS |
| TCAGGTTCGAAACTTCGGCT+CGG | 0.644764 | 4:+28898962 | None:intergenic |
| GTGTCCTAAGAGTATTACCA+TGG | 0.645337 | 4:+28899095 | None:intergenic |
| ATAACCTATTAATCTTCACA+TGG | 0.667379 | 4:+28898114 | None:intergenic |
| TTATCCAATGAGTAAATGGG+AGG | 0.684960 | 4:-28898815 | MsG0480019880.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTAAAGTAATTTTTTTTAT+GGG | - | Chr4:28899050-28899069 | MsG0480019880.01.T01:CDS | 10.0% |
| !!! | TTGTAAAGTAATTTTTTTTA+TGG | - | Chr4:28899049-28899068 | MsG0480019880.01.T01:CDS | 10.0% |
| !!! | GTAAAGTAATTTTTTTTATG+GGG | - | Chr4:28899051-28899070 | MsG0480019880.01.T01:CDS | 15.0% |
| !!! | TTTGATGTTTTTGAAATTTT+AGG | - | Chr4:28899022-28899041 | MsG0480019880.01.T01:CDS | 15.0% |
| !! | AGTTGTTTGAAAACAAAATT+TGG | - | Chr4:28898481-28898500 | MsG0480019880.01.T01:CDS | 20.0% |
| !! | ATATATTGAGGTTAAGAAAA+TGG | - | Chr4:28898648-28898667 | MsG0480019880.01.T01:CDS | 20.0% |
| !!! | AATTTTGTTTTCAAACAACT+TGG | + | Chr4:28898481-28898500 | None:intergenic | 20.0% |
| !!! | ACATTTTTGAATCCAAAATA+AGG | + | Chr4:28898200-28898219 | None:intergenic | 20.0% |
| !!! | ATTTTCTTAACCTCAATATA+TGG | + | Chr4:28898649-28898668 | None:intergenic | 20.0% |
| !!! | CATAAGTTAAGGTTTTTAAA+GGG | - | Chr4:28898526-28898545 | MsG0480019880.01.T01:CDS | 20.0% |
| !!! | TCATAAGTTAAGGTTTTTAA+AGG | - | Chr4:28898525-28898544 | MsG0480019880.01.T01:CDS | 20.0% |
| ! | AGATTATCCAATGAGTAAAT+GGG | - | Chr4:28898273-28898292 | MsG0480019880.01.T01:CDS | 25.0% |
| ! | AGGTTAAGAAAATGGTAAAT+GGG | - | Chr4:28898656-28898675 | MsG0480019880.01.T01:CDS | 25.0% |
| ! | ATAACCTATTAATCTTCACA+TGG | + | Chr4:28898980-28898999 | None:intergenic | 25.0% |
| ! | GTGAAAAAAGTCATAAGTTA+AGG | - | Chr4:28898515-28898534 | MsG0480019880.01.T01:CDS | 25.0% |
| ! | GTGAATGAAATGAAGATAAT+TGG | - | Chr4:28898574-28898593 | MsG0480019880.01.T01:CDS | 25.0% |
| ! | TAGATTATCCAATGAGTAAA+TGG | - | Chr4:28898272-28898291 | MsG0480019880.01.T01:CDS | 25.0% |
| ! | TGTGAAGATTAATAGGTTAT+AGG | - | Chr4:28898980-28898999 | MsG0480019880.01.T01:CDS | 25.0% |
| ! | TTTGAATCCAAAATAAGGAT+AGG | + | Chr4:28898195-28898214 | None:intergenic | 25.0% |
| !! | AAATGAAGATAATTGGTTTC+TGG | - | Chr4:28898581-28898600 | MsG0480019880.01.T01:CDS | 25.0% |
| !! | AATGAAGATAATTGGTTTCT+GGG | - | Chr4:28898582-28898601 | MsG0480019880.01.T01:CDS | 25.0% |
| !! | TTTTACAAAGTTTACTTTGG+AGG | - | Chr4:28898249-28898268 | MsG0480019880.01.T01:CDS | 25.0% |
| !! | TTTTCCATGTGAAGATTAAT+AGG | - | Chr4:28898973-28898992 | MsG0480019880.01.T01:CDS | 25.0% |
| !! | TTTTGGATTCAAAAATGTCT+TGG | - | Chr4:28898202-28898221 | MsG0480019880.01.T01:CDS | 25.0% |
| !!! | ATTGATTCTAAAATCAGTGA+AGG | - | Chr4:28898703-28898722 | MsG0480019880.01.T01:CDS | 25.0% |
| !!! | TGATCTTTTTTCTAAGGTAA+TGG | - | Chr4:28898891-28898910 | MsG0480019880.01.T01:CDS | 25.0% |
| !!! | TTGATTCTAAAATCAGTGAA+GGG | - | Chr4:28898704-28898723 | MsG0480019880.01.T01:CDS | 25.0% |
| GAGGTTAAGAAAATGGTAAA+TGG | - | Chr4:28898655-28898674 | MsG0480019880.01.T01:CDS | 30.0% | |
| TGAGAAAAAACTGATCTTGA+AGG | - | Chr4:28898732-28898751 | MsG0480019880.01.T01:CDS | 30.0% | |
| TGATGTTAAGCCATATATTG+AGG | - | Chr4:28898636-28898655 | MsG0480019880.01.T01:CDS | 30.0% | |
| ! | AAAATGGTAAATGGGTTTGA+TGG | - | Chr4:28898664-28898683 | MsG0480019880.01.T01:CDS | 30.0% |
| ! | ATCAATCAACTCAGTTCTAA+GGG | + | Chr4:28898917-28898936 | None:intergenic | 30.0% |
| ! | GGCTTTTACAAAGTTTACTT+TGG | - | Chr4:28898246-28898265 | MsG0480019880.01.T01:CDS | 30.0% |
| !! | AAGTTTGTGAAGGTATTTGA+TGG | - | Chr4:28898850-28898869 | MsG0480019880.01.T01:CDS | 30.0% |
| !! | GTTAAGGTTTTTAAAGGGTA+AGG | - | Chr4:28898531-28898550 | MsG0480019880.01.T01:CDS | 30.0% |
| !!! | GGTAGATGATCTTTTTTCTA+AGG | - | Chr4:28898885-28898904 | MsG0480019880.01.T01:CDS | 30.0% |
| AAAGGTGAACAAGTTTGTGA+AGG | - | Chr4:28898840-28898859 | MsG0480019880.01.T01:CDS | 35.0% | |
| AAATGATGCAGCAAAGGAAT+TGG | - | Chr4:28898813-28898832 | MsG0480019880.01.T01:CDS | 35.0% | |
| AATCTTACCCTTGATCAACA+AGG | - | Chr4:28898225-28898244 | MsG0480019880.01.T01:CDS | 35.0% | |
| AGCAAAGGAATTGGAGATAA+AGG | - | Chr4:28898822-28898841 | MsG0480019880.01.T01:CDS | 35.0% | |
| AGTGAAAAATGATGCAGCAA+AGG | - | Chr4:28898807-28898826 | MsG0480019880.01.T01:CDS | 35.0% | |
| CTGATAACTCATGAGATTCA+TGG | + | Chr4:28898093-28898112 | None:intergenic | 35.0% | |
| GAAAAAACTGATCTTGAAGG+AGG | - | Chr4:28898735-28898754 | MsG0480019880.01.T01:CDS | 35.0% | |
| GATCAATCAACTCAGTTCTA+AGG | + | Chr4:28898918-28898937 | None:intergenic | 35.0% | |
| GTAGTTGAGATACTCTTCAA+TGG | + | Chr4:28898308-28898327 | None:intergenic | 35.0% | |
| GTTAATCAGGTTCGAAACTT+CGG | + | Chr4:28898137-28898156 | None:intergenic | 35.0% | |
| GTTTGTGGAATCTTATTGTC+TGG | - | Chr4:28898604-28898623 | MsG0480019880.01.T01:CDS | 35.0% | |
| TTATCCAATGAGTAAATGGG+AGG | - | Chr4:28898276-28898295 | MsG0480019880.01.T01:CDS | 35.0% | |
| ! | ATAATTGGTTTCTGGGTTTG+TGG | - | Chr4:28898589-28898608 | MsG0480019880.01.T01:CDS | 35.0% |
| !! | ATTTGATGGTGTAAAAGCAG+AGG | - | Chr4:28898864-28898883 | MsG0480019880.01.T01:CDS | 35.0% |
| !! | CGGAAATCCTATCCTTATTT+TGG | - | Chr4:28898185-28898204 | MsG0480019880.01.T01:CDS | 35.0% |
| !! | CTGAGTTGATTGATCAACTA+AGG | - | Chr4:28898923-28898942 | MsG0480019880.01.T01:CDS | 35.0% |
| !! | CTGATGCTATTTCGTTAATC+AGG | + | Chr4:28898150-28898169 | None:intergenic | 35.0% |
| !! | GTAAAAGCCTTGTTGATCAA+GGG | + | Chr4:28898235-28898254 | None:intergenic | 35.0% |
| !! | TGTAAAAGCCTTGTTGATCA+AGG | + | Chr4:28898236-28898255 | None:intergenic | 35.0% |
| ACTGATCTTGAAGGAGGTAA+AGG | - | Chr4:28898741-28898760 | MsG0480019880.01.T01:CDS | 40.0% | |
| ATAGCATCAGATTCGAAACC+TGG | - | Chr4:28898160-28898179 | MsG0480019880.01.T01:CDS | 40.0% | |
| ATCAGATTCGAAACCTGGAT+CGG | - | Chr4:28898165-28898184 | MsG0480019880.01.T01:CDS | 40.0% | |
| TCAACCTCCCATTTACTCAT+TGG | + | Chr4:28898283-28898302 | None:intergenic | 40.0% | |
| TCCAAAACTCTACAACAAGC+TGG | - | Chr4:28898024-28898043 | MsG0480019880.01.T01:three_prime_UTR | 40.0% | |
| TGAGTTATCAGCATCCTTGA+AGG | - | Chr4:28898102-28898121 | MsG0480019880.01.T01:three_prime_UTR | 40.0% | |
| ! | GGTGATGATCATGATGATGA+TGG | + | Chr4:28898072-28898091 | None:intergenic | 40.0% |
| !! | GATCATGATGATGATGGTTG+TGG | + | Chr4:28898066-28898085 | None:intergenic | 40.0% |
| !!! | TCCAGCTTGTTGTAGAGTTT+TGG | + | Chr4:28898028-28898047 | None:intergenic | 40.0% |
| AAGGATAGGATTTCCGATCC+AGG | + | Chr4:28898181-28898200 | None:intergenic | 45.0% | |
| !! | CATGATGATGATGGTTGTGG+TGG | + | Chr4:28898063-28898082 | None:intergenic | 45.0% |
| TCAGGTTCGAAACTTCGGCT+CGG | + | Chr4:28898132-28898151 | None:intergenic | 50.0% | |
| !! | GATGATGATGGTTGTGGTGG+TGG | + | Chr4:28898060-28898079 | None:intergenic | 50.0% |
| !! | TTCGGCTCGGAAAGCCTTCA+AGG | + | Chr4:28898119-28898138 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 28897998 | 28899115 | 28897998 | ID=MsG0480019880.01;Name=MsG0480019880.01 |
| Chr4 | mRNA | 28897998 | 28899115 | 28897998 | ID=MsG0480019880.01.T01;Parent=MsG0480019880.01;Name=MsG0480019880.01.T01;_AED=0.22;_eAED=0.22;_QI=0|-1|1|1|-1|1|1|158|319 |
| Chr4 | exon | 28897998 | 28899115 | 28897998 | ID=MsG0480019880.01.T01:exon:12064;Parent=MsG0480019880.01.T01 |
| Chr4 | CDS | 28898156 | 28899115 | 28898156 | ID=MsG0480019880.01.T01:cds;Parent=MsG0480019880.01.T01 |
| Chr4 | three_prime_UTR | 28897998 | 28898155 | 28897998 | ID=MsG0480019880.01.T01:three_prime_utr;Parent=MsG0480019880.01.T01 |
| Gene Sequence |
| Protein sequence |
