AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007997.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007997.01.T01 MTR_2g436830 90.038 261 17 2 5 261 40 295 8.14e-155 432
MsG0280007997.01.T01 MTR_4g119820 48.611 288 97 12 1 261 95 358 4.53e-67 212
MsG0280007997.01.T01 MTR_6g045583 51.429 70 28 3 193 261 95 159 6.61e-11 61.2
MsG0280007997.01.T01 MTR_5g049870 51.429 70 28 3 193 261 95 159 7.18e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007997.01.T01 AT1G56210 30.744 309 161 9 1 261 61 364 6.27e-14 71.2
MsG0280007997.01.T01 AT5G27690 46.032 63 34 0 5 67 55 117 7.59e-12 65.1

Find 71 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGACCAGCTTCCTTAAA+AGG 0.155110 2:+24107771 None:intergenic
TGCAAGCTTTGTGCTTCTTT+TGG 0.232522 2:+24107656 None:intergenic
TCTGACCAAGATTCTGAAAT+TGG 0.246160 2:+24107375 None:intergenic
CCTAGCTTTGTTGATCAATA+TGG 0.246316 2:-24107176 MsG0280007997.01.T01:CDS
TGATCAATATGGTACTTATC+AGG 0.301999 2:-24107165 MsG0280007997.01.T01:CDS
GGATTCCAATTTCAGAATCT+TGG 0.332701 2:-24107380 MsG0280007997.01.T01:CDS
TCAGAATCTTGGTCAGACTA+TGG 0.358813 2:-24107369 MsG0280007997.01.T01:CDS
TGTTCACAATCCTCACATAA+TGG 0.367064 2:+24107082 None:intergenic
TTACAATAGGTTCTATCCTA+TGG 0.381858 2:-24107252 MsG0280007997.01.T01:CDS
ATATGTCATCAGCTTACAAT+AGG 0.388071 2:-24107265 MsG0280007997.01.T01:CDS
TTTAGTGATGAAAATGCTAA+TGG 0.402104 2:-24107110 MsG0280007997.01.T01:CDS
CTGGAAAGTACGCTGAGATT+TGG 0.402278 2:-24107748 MsG0280007997.01.T01:CDS
AAAGATGGGTGTAGTGGAAA+TGG 0.408222 2:-24107470 MsG0280007997.01.T01:CDS
CCCTTCCCGTCAAGATTCTC+CGG 0.414067 2:+24107725 None:intergenic
GGAAATGGAAATGGAAATGA+TGG 0.428718 2:-24107449 MsG0280007997.01.T01:CDS
ATCTTGTATCCATCAGAAAC+TGG 0.431923 2:-24107308 MsG0280007997.01.T01:CDS
CACCGGAGGAACATGGTTAC+CGG 0.442748 2:+24107532 None:intergenic
TTTACCATGCATAACTATGA+AGG 0.443995 2:-24107863 None:intergenic
GGTACTTATCAGGTTCAATC+TGG 0.444832 2:-24107155 MsG0280007997.01.T01:CDS
TCATCACTAAACAACTCAAA+AGG 0.460526 2:+24107122 None:intergenic
TTAAGGAAGCTGGTCAAAGC+TGG 0.463380 2:-24107767 MsG0280007997.01.T01:CDS
TTTGACCAGCTTCCTTAAAA+GGG 0.472837 2:+24107772 None:intergenic
GGGTGTAGTGGAAATGGAAA+TGG 0.474373 2:-24107464 MsG0280007997.01.T01:CDS
AGTGGAAATGGAAATGGAAA+TGG 0.477221 2:-24107458 MsG0280007997.01.T01:CDS
AACTAACGCCGGAGAGTCGC+CGG 0.479828 2:-24107567 MsG0280007997.01.T01:CDS
AGCACAAAGCTTGCAAAACA+AGG 0.487035 2:-24107648 MsG0280007997.01.T01:CDS
GAAGATGGAACATGATAAGA+AGG 0.504811 2:+24107215 None:intergenic
TTCTATCCTATGGGAAGAGT+TGG 0.506361 2:-24107242 MsG0280007997.01.T01:CDS
TAGGGCTGAGATTCACTTGG+TGG 0.512971 2:+24107345 None:intergenic
GGCCGGTAACCATGTTCCTC+CGG 0.513408 2:-24107534 MsG0280007997.01.T01:CDS
GTTCCTCCGGTGAGTGGTGG+AGG 0.522411 2:-24107521 MsG0280007997.01.T01:CDS
AATTGGAATCCACTGTGTGC+TGG 0.529577 2:+24107392 None:intergenic
AAGATTGATTGCTGACGTGT+AGG 0.532112 2:+24107326 None:intergenic
TACAATAGGTTCTATCCTAT+GGG 0.533406 2:-24107251 MsG0280007997.01.T01:CDS
ATTTGGCCGGAGAATCTTGA+CGG 0.534632 2:-24107731 MsG0280007997.01.T01:CDS
CCATATTGATCAACAAAGCT+AGG 0.542434 2:+24107176 None:intergenic
GTAACAGTAACTGGAAATGT+TGG 0.543025 2:-24107803 MsG0280007997.01.T01:CDS
AGATTGATTGCTGACGTGTA+GGG 0.544485 2:+24107327 None:intergenic
AACTGGTTATTACCCTCCAA+TGG 0.550201 2:-24107291 MsG0280007997.01.T01:CDS
GGGTAATAACCAGTTTCTGA+TGG 0.551722 2:+24107299 None:intergenic
GCCGGAGAATCTTGACGGGA+AGG 0.553950 2:-24107726 MsG0280007997.01.T01:CDS
GTTACCAAGTGTGAAAATGA+AGG 0.554175 2:-24107614 MsG0280007997.01.T01:CDS
GGACACGTGTAGGGTGAAGA+TGG 0.555418 2:+24107200 None:intergenic
GCTGATGACATATAAACCAT+TGG 0.558867 2:+24107275 None:intergenic
CCGGAGAATCTTGACGGGAA+GGG 0.563881 2:-24107725 MsG0280007997.01.T01:CDS
TTTGGCCGGAGAATCTTGAC+GGG 0.565237 2:-24107730 MsG0280007997.01.T01:CDS
CATGTTCCTCCGGTGAGTGG+TGG 0.568406 2:-24107524 MsG0280007997.01.T01:CDS
AACCATGTTCCTCCGGTGAG+TGG 0.571405 2:-24107527 MsG0280007997.01.T01:CDS
CCTCCGGTGAGTGGTGGAGG+TGG 0.575221 2:-24107518 MsG0280007997.01.T01:CDS
CACCACTCACCGGAGGAACA+TGG 0.583260 2:+24107525 None:intergenic
AAAGTACGCTGAGATTTGGC+CGG 0.585267 2:-24107744 MsG0280007997.01.T01:CDS
AAGCTAGGAGGACACGTGTA+GGG 0.590451 2:+24107191 None:intergenic
AACAACTCAAAAGGAATCAA+CGG 0.594539 2:+24107131 None:intergenic
AGAGTCGCCGGAAAACTCAC+CGG 0.606377 2:-24107555 MsG0280007997.01.T01:CDS
AAAGCTAGGAGGACACGTGT+AGG 0.607435 2:+24107190 None:intergenic
GATGACATATAAACCATTGG+AGG 0.607599 2:+24107278 None:intergenic
AGGGCTGAGATTCACTTGGT+GGG 0.607822 2:+24107346 None:intergenic
GGATCACCAACTCTTCCCAT+AGG 0.609702 2:+24107236 None:intergenic
GAACCACCTCCACCACTCAC+CGG 0.609920 2:+24107515 None:intergenic
AACACCTTCATAGTTATGCA+TGG 0.610166 2:+24107859 None:intergenic
TCGCCGGAAAACTCACCGGC+CGG 0.623716 2:-24107551 MsG0280007997.01.T01:CDS
AATGGAAATGATGGAAGTGA+TGG 0.633579 2:-24107440 MsG0280007997.01.T01:CDS
TTGACTACAGTAGCTAAGAG+TGG 0.651669 2:-24107416 MsG0280007997.01.T01:CDS
ATGACATATAAACCATTGGA+GGG 0.654131 2:+24107279 None:intergenic
GGAGGAACATGGTTACCGGC+CGG 0.661117 2:+24107536 None:intergenic
GCACAAAGCTTGCAAAACAA+GGG 0.661538 2:-24107647 MsG0280007997.01.T01:CDS
CCACCTCCACCACTCACCGG+AGG 0.681711 2:+24107518 None:intergenic
AATGGATGTTCCATTATGTG+AGG 0.691882 2:-24107092 None:intergenic
TATTGATCAACAAAGCTAGG+AGG 0.694963 2:+24107179 None:intergenic
GTGTAGGGCTGAGATTCACT+TGG 0.708981 2:+24107342 None:intergenic
AAGAGTGGACCAGCACACAG+TGG 0.728445 2:-24107401 MsG0280007997.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGAAGAAAAAGAAAGA+TGG - Chr2:24107466-24107485 MsG0280007997.01.T01:CDS 20.0%
!! ATAAGAAGAAAAAGAAAGAT+GGG - Chr2:24107467-24107486 MsG0280007997.01.T01:CDS 20.0%
! TTTAGTGATGAAAATGCTAA+TGG - Chr2:24107841-24107860 MsG0280007997.01.T01:CDS 25.0%
AAAAAGTCAAAAACTAACGC+CGG - Chr2:24107373-24107392 MsG0280007997.01.T01:CDS 30.0%
AACAACTCAAAAGGAATCAA+CGG + Chr2:24107823-24107842 None:intergenic 30.0%
ATATGTCATCAGCTTACAAT+AGG - Chr2:24107686-24107705 MsG0280007997.01.T01:CDS 30.0%
ATGACATATAAACCATTGGA+GGG + Chr2:24107675-24107694 None:intergenic 30.0%
CAAAGAGAAGAAAAAGAACA+AGG - Chr2:24107261-24107280 MsG0280007997.01.T01:CDS 30.0%
CAACAAAAAGTAACAGTAAC+TGG - Chr2:24107139-24107158 MsG0280007997.01.T01:CDS 30.0%
TACAATAGGTTCTATCCTAT+GGG - Chr2:24107700-24107719 MsG0280007997.01.T01:CDS 30.0%
TCATCACTAAACAACTCAAA+AGG + Chr2:24107832-24107851 None:intergenic 30.0%
TGATCAATATGGTACTTATC+AGG - Chr2:24107786-24107805 MsG0280007997.01.T01:CDS 30.0%
TTACAATAGGTTCTATCCTA+TGG - Chr2:24107699-24107718 MsG0280007997.01.T01:CDS 30.0%
! TTTACCTTCATTTTCACACT+TGG + Chr2:24107344-24107363 None:intergenic 30.0%
AAAAAGAAAGATGGGTGTAG+TGG - Chr2:24107475-24107494 MsG0280007997.01.T01:CDS 35.0%
AGTGGAAATGGAAATGGAAA+TGG - Chr2:24107493-24107512 MsG0280007997.01.T01:CDS 35.0%
ATCTTGTATCCATCAGAAAC+TGG - Chr2:24107643-24107662 MsG0280007997.01.T01:CDS 35.0%
CCATATTGATCAACAAAGCT+AGG + Chr2:24107778-24107797 None:intergenic 35.0%
GAAGATGGAACATGATAAGA+AGG + Chr2:24107739-24107758 None:intergenic 35.0%
GATGACATATAAACCATTGG+AGG + Chr2:24107676-24107695 None:intergenic 35.0%
GCTGATGACATATAAACCAT+TGG + Chr2:24107679-24107698 None:intergenic 35.0%
GGAAATGGAAATGGAAATGA+TGG - Chr2:24107502-24107521 MsG0280007997.01.T01:CDS 35.0%
GGATTCCAATTTCAGAATCT+TGG - Chr2:24107571-24107590 MsG0280007997.01.T01:CDS 35.0%
GTAACAGTAACTGGAAATGT+TGG - Chr2:24107148-24107167 MsG0280007997.01.T01:CDS 35.0%
GTTACCAAGTGTGAAAATGA+AGG - Chr2:24107337-24107356 MsG0280007997.01.T01:CDS 35.0%
TATTGATCAACAAAGCTAGG+AGG + Chr2:24107775-24107794 None:intergenic 35.0%
TTTGACCAGCTTCCTTAAAA+GGG + Chr2:24107182-24107201 None:intergenic 35.0%
! AATGGAAATGATGGAAGTGA+TGG - Chr2:24107511-24107530 MsG0280007997.01.T01:CDS 35.0%
! GTTCTTTTTCTTCTCTTTGC+CGG + Chr2:24107260-24107279 None:intergenic 35.0%
!! CCTAGCTTTGTTGATCAATA+TGG - Chr2:24107775-24107794 MsG0280007997.01.T01:CDS 35.0%
!! TCTGACCAAGATTCTGAAAT+TGG + Chr2:24107579-24107598 None:intergenic 35.0%
AACTGGTTATTACCCTCCAA+TGG - Chr2:24107660-24107679 MsG0280007997.01.T01:CDS 40.0%
AAGATTGATTGCTGACGTGT+AGG + Chr2:24107628-24107647 None:intergenic 40.0%
AGATTGATTGCTGACGTGTA+GGG + Chr2:24107627-24107646 None:intergenic 40.0%
AGCACAAAGCTTGCAAAACA+AGG - Chr2:24107303-24107322 MsG0280007997.01.T01:CDS 40.0%
CTTTGACCAGCTTCCTTAAA+AGG + Chr2:24107183-24107202 None:intergenic 40.0%
GCACAAAGCTTGCAAAACAA+GGG - Chr2:24107304-24107323 MsG0280007997.01.T01:CDS 40.0%
GGGTAATAACCAGTTTCTGA+TGG + Chr2:24107655-24107674 None:intergenic 40.0%
GGTACTTATCAGGTTCAATC+TGG - Chr2:24107796-24107815 MsG0280007997.01.T01:CDS 40.0%
TCAGAATCTTGGTCAGACTA+TGG - Chr2:24107582-24107601 MsG0280007997.01.T01:CDS 40.0%
TTCTATCCTATGGGAAGAGT+TGG - Chr2:24107709-24107728 MsG0280007997.01.T01:CDS 40.0%
TTGACTACAGTAGCTAAGAG+TGG - Chr2:24107535-24107554 MsG0280007997.01.T01:CDS 40.0%
! AAAGATGGGTGTAGTGGAAA+TGG - Chr2:24107481-24107500 MsG0280007997.01.T01:CDS 40.0%
!! TGCAAGCTTTGTGCTTCTTT+TGG + Chr2:24107298-24107317 None:intergenic 40.0%
!!! TTGGTGTTGAGACCCTTTTA+AGG - Chr2:24107167-24107186 MsG0280007997.01.T01:CDS 40.0%
AAAGTACGCTGAGATTTGGC+CGG - Chr2:24107207-24107226 MsG0280007997.01.T01:CDS 45.0%
AATTGGAATCCACTGTGTGC+TGG + Chr2:24107562-24107581 None:intergenic 45.0%
ATTTGGCCGGAGAATCTTGA+CGG - Chr2:24107220-24107239 MsG0280007997.01.T01:CDS 45.0%
TTAAGGAAGCTGGTCAAAGC+TGG - Chr2:24107184-24107203 MsG0280007997.01.T01:CDS 45.0%
! CTGGAAAGTACGCTGAGATT+TGG - Chr2:24107203-24107222 MsG0280007997.01.T01:CDS 45.0%
! GGGTGTAGTGGAAATGGAAA+TGG - Chr2:24107487-24107506 MsG0280007997.01.T01:CDS 45.0%
!! TGAGACCCTTTTAAGGAAGC+TGG - Chr2:24107174-24107193 MsG0280007997.01.T01:CDS 45.0%
AAAGCTAGGAGGACACGTGT+AGG + Chr2:24107764-24107783 None:intergenic 50.0%
AAGCTAGGAGGACACGTGTA+GGG + Chr2:24107763-24107782 None:intergenic 50.0%
AGGGCTGAGATTCACTTGGT+GGG + Chr2:24107608-24107627 None:intergenic 50.0%
GGATCACCAACTCTTCCCAT+AGG + Chr2:24107718-24107737 None:intergenic 50.0%
GTGTAGGGCTGAGATTCACT+TGG + Chr2:24107612-24107631 None:intergenic 50.0%
TAGGGCTGAGATTCACTTGG+TGG + Chr2:24107609-24107628 None:intergenic 50.0%
TTTGGCCGGAGAATCTTGAC+GGG - Chr2:24107221-24107240 MsG0280007997.01.T01:CDS 50.0%
AACCATGTTCCTCCGGTGAG+TGG - Chr2:24107424-24107443 MsG0280007997.01.T01:CDS 55.0%
AAGAGTGGACCAGCACACAG+TGG - Chr2:24107550-24107569 MsG0280007997.01.T01:CDS 55.0%
AGAGTCGCCGGAAAACTCAC+CGG - Chr2:24107396-24107415 MsG0280007997.01.T01:CDS 55.0%
CACCGGAGGAACATGGTTAC+CGG + Chr2:24107422-24107441 None:intergenic 55.0%
CCCTTCCCGTCAAGATTCTC+CGG + Chr2:24107229-24107248 None:intergenic 55.0%
CCGGAGAATCTTGACGGGAA+GGG - Chr2:24107226-24107245 MsG0280007997.01.T01:CDS 55.0%
GACGGGAAGGGAAAAAGCTC+CGG - Chr2:24107238-24107257 MsG0280007997.01.T01:CDS 55.0%
GGACACGTGTAGGGTGAAGA+TGG + Chr2:24107754-24107773 None:intergenic 55.0%
! TGAGTTTTCCGGCGACTCTC+CGG + Chr2:24107395-24107414 None:intergenic 55.0%
! TTACCGGCCGGTGAGTTTTC+CGG + Chr2:24107406-24107425 None:intergenic 55.0%
AACTAACGCCGGAGAGTCGC+CGG - Chr2:24107384-24107403 MsG0280007997.01.T01:CDS 60.0%
CACCACTCACCGGAGGAACA+TGG + Chr2:24107429-24107448 None:intergenic 60.0%
CATGTTCCTCCGGTGAGTGG+TGG - Chr2:24107427-24107446 MsG0280007997.01.T01:CDS 60.0%
GAACCACCTCCACCACTCAC+CGG + Chr2:24107439-24107458 None:intergenic 60.0%
GCCGGAGAATCTTGACGGGA+AGG - Chr2:24107225-24107244 MsG0280007997.01.T01:CDS 60.0%
GGAGGAACATGGTTACCGGC+CGG + Chr2:24107418-24107437 None:intergenic 60.0%
GGCCGGTAACCATGTTCCTC+CGG - Chr2:24107417-24107436 MsG0280007997.01.T01:CDS 60.0%
GTTCCTCCGGTGAGTGGTGG+AGG - Chr2:24107430-24107449 MsG0280007997.01.T01:CDS 65.0%
TCGCCGGAAAACTCACCGGC+CGG - Chr2:24107400-24107419 MsG0280007997.01.T01:CDS 65.0%
CCACCTCCACCACTCACCGG+AGG + Chr2:24107436-24107455 None:intergenic 70.0%
! CCTCCGGTGAGTGGTGGAGG+TGG - Chr2:24107433-24107452 MsG0280007997.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 24107094 24107879 24107094 ID=MsG0280007997.01;Name=MsG0280007997.01
Chr2 mRNA 24107094 24107879 24107094 ID=MsG0280007997.01.T01;Parent=MsG0280007997.01;Name=MsG0280007997.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|261
Chr2 exon 24107094 24107879 24107094 ID=MsG0280007997.01.T01:exon:40551;Parent=MsG0280007997.01.T01
Chr2 CDS 24107094 24107879 24107094 ID=MsG0280007997.01.T01:cds;Parent=MsG0280007997.01.T01
Gene Sequence

>MsG0280007997.01.T01

ATGCATAACTATGAAGGTGTTTTCACTGCAACAATTGATTCACAACAACAAAAAGTAACAGTAACTGGAAATGTTGGTGTTGAGACCCTTTTAAGGAAGCTGGTCAAAGCTGGAAAGTACGCTGAGATTTGGCCGGAGAATCTTGACGGGAAGGGAAAAAGCTCCGGCAAAGAGAAGAAAAAGAACAAGGATCAGAATGAACCAAAAGAAGCACAAAGCTTGCAAAACAAGGGAACTGAAAGTGTTACCAAGTGTGAAAATGAAGGTAAAAACAAGAACAAAAAGTCAAAAACTAACGCCGGAGAGTCGCCGGAAAACTCACCGGCCGGTAACCATGTTCCTCCGGTGAGTGGTGGAGGTGGTTCTGATAATAATAAGAAGAAAAAGAAAGATGGGTGTAGTGGAAATGGAAATGGAAATGGAAATGATGGAAGTGATGGTTTGACTACAGTAGCTAAGAGTGGACCAGCACACAGTGGATTCCAATTTCAGAATCTTGGTCAGACTATGGCCCACCAAGTGAATCTCAGCCCTACACGTCAGCAATCAATCTTGTATCCATCAGAAACTGGTTATTACCCTCCAATGGTTTATATGTCATCAGCTTACAATAGGTTCTATCCTATGGGAAGAGTTGGTGATCCTTCTTATCATGTTCCATCTTCACCCTACACGTGTCCTCCTAGCTTTGTTGATCAATATGGTACTTATCAGGTTCAATCTGGACCGTTGATTCCTTTTGAGTTGTTTAGTGATGAAAATGCTAATGGATGTTCCATTATGTGA

Protein sequence

>MsG0280007997.01.T01

MHNYEGVFTATIDSQQQKVTVTGNVGVETLLRKLVKAGKYAEIWPENLDGKGKSSGKEKKKNKDQNEPKEAQSLQNKGTESVTKCENEGKNKNKKSKTNAGESPENSPAGNHVPPVSGGGGSDNNKKKKKDGCSGNGNGNGNDGSDGLTTVAKSGPAHSGFQFQNLGQTMAHQVNLSPTRQQSILYPSETGYYPPMVYMSSAYNRFYPMGRVGDPSYHVPSSPYTCPPSFVDQYGTYQVQSGPLIPFELFSDENANGCSIM*