AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028436.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028436.01.T01 MTR_5g071090 83.019 318 18 5 25 338 27 312 2.37e-171 478
MsG0580028436.01.T01 MTR_3g464300 35.294 391 163 12 26 335 27 408 3.03e-47 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATGAGTGTGTAGATATT+TGG 0.183054 5:-80494028 MsG0580028436.01.T01:CDS
TGGTGTAGTTGCAAGGTTAA+TGG 0.255423 5:-80495022 MsG0580028436.01.T01:CDS
AAGAGTTTGTTTAAGGAAGT+TGG 0.287665 5:-80494745 MsG0580028436.01.T01:CDS
GGTGTAGTTGCAAGGTTAAT+GGG 0.347018 5:-80495021 MsG0580028436.01.T01:CDS
TGAAGAAGAGAGAAAAGTTA+AGG 0.378603 5:-80494099 MsG0580028436.01.T01:CDS
TCAAAATCAAAGACAGAAAC+AGG 0.397845 5:+80494631 None:intergenic
GAGCATGATGTTTCTGGAAC+AGG 0.399353 5:-80494610 MsG0580028436.01.T01:intron
AGTTGCAAGGTTAATGGGAT+TGG 0.401363 5:-80495016 MsG0580028436.01.T01:CDS
CAAGCTAGTTGATGATGAAT+TGG 0.402114 5:-80493994 MsG0580028436.01.T01:CDS
CAAAGGAAGAAGAAAAGAAA+AGG 0.402477 5:-80494836 MsG0580028436.01.T01:CDS
GAAATTATAGTTCTAGTAGT+AGG 0.402687 5:+80495057 None:intergenic
GTTAATGGGATTGGATTCAA+TGG 0.432909 5:-80495007 MsG0580028436.01.T01:CDS
ATTGAGAACAATTTGAATGA+AGG 0.441642 5:-80494074 MsG0580028436.01.T01:CDS
CTTCTTCTGGTGTAGTTGCA+AGG 0.448678 5:-80495029 MsG0580028436.01.T01:CDS
TTGTTTAAGGAAGTTGGAAA+TGG 0.464241 5:-80494739 MsG0580028436.01.T01:CDS
ATTATAGTTCTAGTAGTAGG+AGG 0.474477 5:+80495060 None:intergenic
TTTGTGATGTTGATATGAGT+TGG 0.497412 5:-80494193 MsG0580028436.01.T01:CDS
GTGAATTGCATGAAGTAAAG+AGG 0.512937 5:-80493962 MsG0580028436.01.T01:CDS
TTGCAATGTGTAAGTGAACT+TGG 0.522297 5:+80494958 None:intergenic
GGATTGGATTCAATGGAAGA+AGG 0.529487 5:-80495000 MsG0580028436.01.T01:CDS
CTAGTTGATGATGAATTGGT+TGG 0.537429 5:-80493990 MsG0580028436.01.T01:CDS
CAGAATGTTGAATAAAGAGC+TGG 0.546875 5:+80494138 None:intergenic
AGTAGAAAATTCAAGTCCAA+AGG 0.548034 5:-80494853 MsG0580028436.01.T01:CDS
AAAGGAAGAAGAAAAGAAAA+GGG 0.548330 5:-80494835 MsG0580028436.01.T01:CDS
GATTGGATTCAATGGAAGAA+GGG 0.549935 5:-80494999 MsG0580028436.01.T01:CDS
TTGGTGTTTAGCTTTGAAAG+TGG 0.552499 5:-80494883 MsG0580028436.01.T01:CDS
TTATAGTTCTAGTAGTAGGA+GGG 0.559285 5:+80495061 None:intergenic
TCAAGCAAATGAAAAGCTTG+TGG 0.565993 5:+80494919 None:intergenic
AACATGTTGTGTTGAAGAGA+AGG 0.571785 5:-80494686 MsG0580028436.01.T01:CDS
CTAAAGTTTGATGAAGATGA+TGG 0.588538 5:+80495210 None:intergenic
AGAGAAAAGGTGAATGATGA+TGG 0.597704 5:-80494784 MsG0580028436.01.T01:CDS
GTGTTTAGCTTTGAAAGTGG+AGG 0.617135 5:-80494880 MsG0580028436.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTATTTCCAAAAAATAAAA+AGG + Chr5:80494719-80494738 None:intergenic 15.0%
!! CAAATTTAATACATGGAAAA+CGG + Chr5:80494853-80494872 None:intergenic 20.0%
!! TAGTTAGCAAATTTAATACA+TGG + Chr5:80494860-80494879 None:intergenic 20.0%
!!! ATTATTTCAACAAGCTTTTA+AGG - Chr5:80494639-80494658 MsG0580028436.01.T01:CDS 20.0%
!!! GATCTTACCTTTTTATTTTT+TGG - Chr5:80494709-80494728 MsG0580028436.01.T01:CDS 20.0%
! AAAGGAAGAAGAAAAGAAAA+GGG - Chr5:80494271-80494290 MsG0580028436.01.T01:intron 25.0%
! AATTTAATGCATGTTTGGAT+TGG - Chr5:80494567-80494586 MsG0580028436.01.T01:intron 25.0%
! ATAAACAATTAGGTCATGTT+TGG + Chr5:80494780-80494799 None:intergenic 25.0%
! ATAATTTCTTCTTCTTCTTC+TGG - Chr5:80494064-80494083 MsG0580028436.01.T01:CDS 25.0%
! ATTGAGAACAATTTGAATGA+AGG - Chr5:80495032-80495051 MsG0580028436.01.T01:CDS 25.0%
! CACTATGTATCTAAATTCTA+AGG + Chr5:80494817-80494836 None:intergenic 25.0%
! TTCTCTAATCCTATGAAAAA+TGG + Chr5:80493975-80493994 None:intergenic 25.0%
! TTGTGTTTGCATAAACAATT+AGG + Chr5:80494790-80494809 None:intergenic 25.0%
!!! GAAATTATAGTTCTAGTAGT+AGG + Chr5:80494052-80494071 None:intergenic 25.0%
!!! TTTTGAGAAGAGTTTGTTTA+AGG - Chr5:80494354-80494373 MsG0580028436.01.T01:intron 25.0%
AAGGATCAAAGAAAAAAGAG+AGG - Chr5:80495051-80495070 MsG0580028436.01.T01:CDS 30.0%
ACAATGAGTGTGTAGATATT+TGG - Chr5:80495078-80495097 MsG0580028436.01.T01:CDS 30.0%
AGTAGAAAATTCAAGTCCAA+AGG - Chr5:80494253-80494272 MsG0580028436.01.T01:intron 30.0%
CAAAGGAAGAAGAAAAGAAA+AGG - Chr5:80494270-80494289 MsG0580028436.01.T01:intron 30.0%
CATATATAAACATGTTGCAG+AGG - Chr5:80494884-80494903 MsG0580028436.01.T01:CDS 30.0%
CCAAACATGCATTAAATTTG+AGG + Chr5:80494565-80494584 None:intergenic 30.0%
CCTCAAATTTAATGCATGTT+TGG - Chr5:80494562-80494581 MsG0580028436.01.T01:intron 30.0%
CTAAAGTTTGATGAAGATGA+TGG + Chr5:80493899-80493918 None:intergenic 30.0%
CTATTGAAATGCTCATAAGT+TGG - Chr5:80494610-80494629 MsG0580028436.01.T01:intron 30.0%
GAAGAAAAACAAGAGAGAAA+AGG - Chr5:80494309-80494328 MsG0580028436.01.T01:intron 30.0%
TCAAAATCAAAGACAGAAAC+AGG + Chr5:80494478-80494497 None:intergenic 30.0%
TGAAGAAGAGAGAAAAGTTA+AGG - Chr5:80495007-80495026 MsG0580028436.01.T01:CDS 30.0%
TTAAGGATAGCTTACGAAAA+CGG - Chr5:80494656-80494675 MsG0580028436.01.T01:CDS 30.0%
TTGTTTAAGGAAGTTGGAAA+TGG - Chr5:80494367-80494386 MsG0580028436.01.T01:intron 30.0%
TTTGTGATGTTGATATGAGT+TGG - Chr5:80494913-80494932 MsG0580028436.01.T01:CDS 30.0%
! AAGAGTTTGTTTAAGGAAGT+TGG - Chr5:80494361-80494380 MsG0580028436.01.T01:intron 30.0%
! CTTTTCTTTTCTTCTTCCTT+TGG + Chr5:80494272-80494291 None:intergenic 30.0%
! GCTTGAAAATGAGAACTTTT+TGG - Chr5:80494204-80494223 MsG0580028436.01.T01:CDS 30.0%
!! ATTATAGTTCTAGTAGTAGG+AGG + Chr5:80494049-80494068 None:intergenic 30.0%
!! GTTTCACAACCATTTTTCAT+AGG - Chr5:80493963-80493982 MsG0580028436.01.T01:CDS 30.0%
!! TTATAGTTCTAGTAGTAGGA+GGG + Chr5:80494048-80494067 None:intergenic 30.0%
AACATGTTGTGTTGAAGAGA+AGG - Chr5:80494420-80494439 MsG0580028436.01.T01:intron 35.0%
AGAGAAAAGGTGAATGATGA+TGG - Chr5:80494322-80494341 MsG0580028436.01.T01:intron 35.0%
CAGAATGTTGAATAAAGAGC+TGG + Chr5:80494971-80494990 None:intergenic 35.0%
GATTGGATTCAATGGAAGAA+GGG - Chr5:80494107-80494126 MsG0580028436.01.T01:CDS 35.0%
GTGAATTGCATGAAGTAAAG+AGG - Chr5:80495144-80495163 MsG0580028436.01.T01:CDS 35.0%
GTTAATGGGATTGGATTCAA+TGG - Chr5:80494099-80494118 MsG0580028436.01.T01:CDS 35.0%
TCAAGCAAATGAAAAGCTTG+TGG + Chr5:80494190-80494209 None:intergenic 35.0%
TTGCAATGTGTAAGTGAACT+TGG + Chr5:80494151-80494170 None:intergenic 35.0%
! GATTTTGAGCATGATGTTTC+TGG - Chr5:80494490-80494509 MsG0580028436.01.T01:intron 35.0%
!! CAAGCTAGTTGATGATGAAT+TGG - Chr5:80495112-80495131 MsG0580028436.01.T01:CDS 35.0%
!! CTAGTTGATGATGAATTGGT+TGG - Chr5:80495116-80495135 MsG0580028436.01.T01:CDS 35.0%
!! TTGGTGTTTAGCTTTGAAAG+TGG - Chr5:80494223-80494242 MsG0580028436.01.T01:intron 35.0%
AGTTGCAAGGTTAATGGGAT+TGG - Chr5:80494090-80494109 MsG0580028436.01.T01:CDS 40.0%
GGATTGGATTCAATGGAAGA+AGG - Chr5:80494106-80494125 MsG0580028436.01.T01:CDS 40.0%
GGTGTAGTTGCAAGGTTAAT+GGG - Chr5:80494085-80494104 MsG0580028436.01.T01:CDS 40.0%
TGGTGTAGTTGCAAGGTTAA+TGG - Chr5:80494084-80494103 MsG0580028436.01.T01:CDS 40.0%
!! GTGTTTAGCTTTGAAAGTGG+AGG - Chr5:80494226-80494245 MsG0580028436.01.T01:intron 40.0%
AGCTCAAAAACTGTTGCTGC+AGG - Chr5:80493938-80493957 MsG0580028436.01.T01:CDS 45.0%
CTTCTTCTGGTGTAGTTGCA+AGG - Chr5:80494077-80494096 MsG0580028436.01.T01:CDS 45.0%
GCTCAAAAACTGTTGCTGCA+GGG - Chr5:80493939-80493958 MsG0580028436.01.T01:CDS 45.0%
!! AGCTGGTCATTTTCAAGCAC+TGG + Chr5:80494954-80494973 None:intergenic 45.0%
!! GAGCATGATGTTTCTGGAAC+AGG - Chr5:80494496-80494515 MsG0580028436.01.T01:intron 45.0%
Chromosome Type Strat End Strand Name
Chr5 gene 80493863 80495265 80493863 ID=MsG0580028436.01;Name=MsG0580028436.01
Chr5 mRNA 80493863 80495265 80493863 ID=MsG0580028436.01.T01;Parent=MsG0580028436.01;Name=MsG0580028436.01.T01;_AED=0.23;_eAED=0.23;_QI=0|0|0|1|1|1|2|0|338
Chr5 exon 80494611 80495265 80494611 ID=MsG0580028436.01.T01:exon:23753;Parent=MsG0580028436.01.T01
Chr5 exon 80493863 80494224 80493863 ID=MsG0580028436.01.T01:exon:23752;Parent=MsG0580028436.01.T01
Chr5 CDS 80494611 80495265 80494611 ID=MsG0580028436.01.T01:cds;Parent=MsG0580028436.01.T01
Chr5 CDS 80493863 80494224 80493863 ID=MsG0580028436.01.T01:cds;Parent=MsG0580028436.01.T01
Gene Sequence

>MsG0580028436.01.T01

ATGAAGCTCTTGATATCATCTTATTCATCATCACCATCATCTTCATCAAACTTTAGCTTTGACTCAACATTTTGCAGCTCAAAAACTGTTGCTGCAGGGTGTTTCACAACCATTTTTCATAGGATTAGAGAATTTCATTCAAAGTTCATTGTAAGTGAAAAAGTTCAAAAATTGAAGACTAACCCTCCTACTACTAGAACTATAATTTCTTCTTCTTCTTCTGGTGTAGTTGCAAGGTTAATGGGATTGGATTCAATGGAAGAAGGGATAGTTTCTGAATCAAAACCAAGTTCACTTACACATTGCAAGTCATTGAACTATGTGCCACAAGCTTTTCATTTGCTTGAAAATGAGAACTTTTTGGTGTTTAGCTTTGAAAGTGGAGGTGAGAGTAGAAAATTCAAGTCCAAAGGAAGAAGAAAAGAAAAGGGTTGTGTTGAATTGAAGAAGAAAAACAAGAGAGAAAAGGTGAATGATGATGGTGAATTTCATTTTGAGAAGAGTTTGTTTAAGGAAGTTGGAAATGGTGAGAAAGTGAAGAAGAGAAAGAAAGAAAAAACATGTTGTGTTGAAGAGAAGGTTGAAAGTGAATGTAGTTCAGAAGATTATAGTCCTGTTTCTGTCTTTGATTTTGAGCATGATGTTTCTGGAACAGAGGAAGATCTTTGTGATGTTGATATGAGTTGGAGAAGAAAATTGTCACCAGTGCTTGAAAATGACCAGCTCTTTATTCAACATTCTGATAGTAACTTAATAAATGAAGAAGAGAGAAAAGTTAAGGAAATTGAGAACAATTTGAATGAAGGATCAAAGAAAAAAGAGAGGCATAACAATGAGTGTGTAGATATTTGGATTAAAATTTGCAAGCTAGTTGATGATGAATTGGTTGGATCAAGTGAATTGCATGAAGTAAAGAGGAAACAAAATGATTTAGAAAATATAAGTGCTGATTTTGAGTTAGAGATTTTAGATGAATTGTTAGATGAAGTTATAGATCAATTTGTTAGTTGTTTGTAA

Protein sequence

>MsG0580028436.01.T01

MKLLISSYSSSPSSSSNFSFDSTFCSSKTVAAGCFTTIFHRIREFHSKFIVSEKVQKLKTNPPTTRTIISSSSSGVVARLMGLDSMEEGIVSESKPSSLTHCKSLNYVPQAFHLLENENFLVFSFESGGESRKFKSKGRRKEKGCVELKKKNKREKVNDDGEFHFEKSLFKEVGNGEKVKKRKKEKTCCVEEKVESECSSEDYSPVSVFDFEHDVSGTEEDLCDVDMSWRRKLSPVLENDQLFIQHSDSNLINEEERKVKEIENNLNEGSKKKERHNNECVDIWIKICKLVDDELVGSSELHEVKRKQNDLENISADFELEILDELLDEVIDQFVSCL*