AlfalfaGEDB | MsG0280011319.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011319.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011319.01.T01	XP_003597762.1	100	66	0	0	1	66	138	203	6.43E-38	134

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011319.01.T01	G7II32	100.000	66	0	0	1	66	138	203	3.07e-38	134

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001892.01	MsG0280011319.01	0.802635	5.108487e-49	2.514974e-46
MsG0180003094.01	MsG0280011319.01	0.805616	1.224684e-49	6.508909e-47
MsG0180003403.01	MsG0280011319.01	0.807580	4.716228e-50	2.636411e-47
MsG0180005153.01	MsG0280011319.01	0.823606	1.265013e-53	1.094410e-50
MsG0180006017.01	MsG0280011319.01	0.803144	4.010510e-49	2.000167e-46
MsG0280011319.01	MsG0480021056.01	0.821521	3.864024e-53	3.150770e-50
MsG0280011319.01	MsG0480022054.01	0.809335	1.991069e-50	1.165826e-47
MsG0280011319.01	MsG0580025332.01	0.801787	7.636152e-49	3.679059e-46
MsG0280011319.01	MsG0580025587.01	0.802051	6.740263e-49	3.269326e-46
MsG0280011319.01	MsG0580027925.01	0.811895	5.568015e-51	3.488496e-48
MsG0280011319.01	MsG0580028436.01	0.801982	6.962404e-49	3.371009e-46
MsG0280011319.01	MsG0580029236.01	0.815484	9.030282e-52	6.234218e-49
MsG0280011319.01	MsG0680030447.01	0.809544	1.795276e-50	1.057062e-47
MsG0280011319.01	MsG0680031431.01	0.816548	5.225087e-52	3.713386e-49
MsG0280011319.01	MsG0680031621.01	0.808141	3.582628e-50	2.032586e-47
MsG0280011319.01	MsG0680035498.01	0.818435	1.963492e-52	1.469755e-49
MsG0280011319.01	MsG0780036766.01	0.800638	1.312224e-48	6.141125e-46
MsG0280011319.01	MsG0780041083.01	0.808459	3.065064e-50	1.753567e-47
MsG0280011319.01	MsG0780041440.01	0.813089	3.053335e-51	1.974997e-48
MsG0280011319.01	MsG0780041448.01	0.805873	1.081933e-49	5.788468e-47
MsG0280011319.01	MsG0780041699.01	0.801207	1.003865e-48	4.766242e-46
MsG0280011319.01	MsG0880042311.01	0.802359	5.824449e-49	2.847503e-46
MsG0280007506.01	MsG0280011319.01	0.809637	1.714667e-50	1.012151e-47
MsG0280007955.01	MsG0280011319.01	0.823053	1.703637e-53	1.451145e-50
MsG0280010349.01	MsG0280011319.01	0.801295	9.631483e-49	4.583145e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011319.01.T01	MTR_2g101980	100.000	66	0	0	1	66	138	203	7.79e-42	134
MsG0280011319.01.T01	MTR_0147s0050	62.121	66	23	1	1	66	139	202	1.57e-22	85.5
MsG0280011319.01.T01	MTR_0147s0020	70.588	51	15	0	1	51	17	67	3.18e-21	80.9
MsG0280011319.01.T01	MTR_8g103530	46.970	66	31	2	1	65	140	202	1.97e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011319.01.T01	AT3G16070	68.293	41	13	0	1	41	110	150	1.16e-16	69.3
MsG0280011319.01.T01	AT1G15260	51.515	66	30	1	1	66	115	178	4.37e-15	65.9

Find 8 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACAATATCTAAATAAAT+TGG	0.262357	2:-82364179	None:intergenic
GAATTGGAGATGAATGATTC+AGG	0.388996	2:+82364095	None:intergenic
TCTAAGTCAAAGGGAAGATT+TGG	0.402476	2:+82364266	MsG0280011319.01.T01:CDS
TCCAATTTATTTAGATATTG+TGG	0.422256	2:+82364178	MsG0280011319.01.T01:CDS
AGGGAAGATTTGGATCAATC+AGG	0.432753	2:+82364276	MsG0280011319.01.T01:CDS
ATCAAACGCTCTAAGTCAAA+GGG	0.509484	2:+82364257	MsG0280011319.01.T01:CDS
CATCAAACGCTCTAAGTCAA+AGG	0.517132	2:+82364256	MsG0280011319.01.T01:CDS
AGAGCGTTTGATGCTGACAG+AGG	0.645792	2:-82364246	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCCAATTTATTTAGATATTG+TGG	+	Chr2:82364178-82364197	MsG0280011319.01.T01:CDS	20.0%
!!	TCCACAATATCTAAATAAAT+TGG	-	Chr2:82364182-82364201	None:intergenic	20.0%
	TCTAAGTCAAAGGGAAGATT+TGG	+	Chr2:82364266-82364285	MsG0280011319.01.T01:CDS	35.0%
!	ATCAAACGCTCTAAGTCAAA+GGG	+	Chr2:82364257-82364276	MsG0280011319.01.T01:CDS	35.0%
	AGGGAAGATTTGGATCAATC+AGG	+	Chr2:82364276-82364295	MsG0280011319.01.T01:CDS	40.0%
!	CATCAAACGCTCTAAGTCAA+AGG	+	Chr2:82364256-82364275	MsG0280011319.01.T01:CDS	40.0%
!	AGAGCGTTTGATGCTGACAG+AGG	-	Chr2:82364249-82364268	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	82364104	82364304	82364104	ID=MsG0280011319.01;Name=MsG0280011319.01
Chr2	mRNA	82364104	82364304	82364104	ID=MsG0280011319.01.T01;Parent=MsG0280011319.01;Name=MsG0280011319.01.T01;_AED=0.35;_eAED=0.35;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|66
Chr2	exon	82364104	82364304	82364104	ID=MsG0280011319.01.T01:exon:22570;Parent=MsG0280011319.01.T01
Chr2	CDS	82364104	82364304	82364104	ID=MsG0280011319.01.T01:cds;Parent=MsG0280011319.01.T01

Gene Sequence	>MsG0280011319.01.T01 ATGAATGATTCAGGTGATGTTGAACAAGTACTTGACATAGAAGAAGCACTTCACTATTATTCTCGTCTCAAGAGTCCAATTTATTTAGATATTGTGGACAAGTTTTTCACTGACATGCACTCAGAATTGTCTGTTTCACAATCCTCTGTCAGCATCAAACGCTCTAAGTCAAAGGGAAGATTTGGATCAATCAGGTTGTAG
Protein sequence	>MsG0280011319.01.T01 MNDSGDVEQVLDIEEALHYYSRLKSPIYLDIVDKFFTDMHSELSVSQSSVSIKRSKSKGRFGSIRL*