Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041448.01.T01 | XP_003626123.1 | 97.949 | 585 | 8 | 2 | 7 | 588 | 2 | 585 | 0 | 1165 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000038.01 | MsG0780041448.01 | 0.834307 | 3.232604e-56 | 3.821397e-53 |
| MsG0180000404.01 | MsG0780041448.01 | 0.810676 | 1.024055e-50 | 6.212293e-48 |
| MsG0180003403.01 | MsG0780041448.01 | 0.807160 | 5.788602e-50 | 3.200903e-47 |
| MsG0180003741.01 | MsG0780041448.01 | 0.829154 | 6.031854e-55 | 6.122284e-52 |
| MsG0180003951.01 | MsG0780041448.01 | 0.820372 | 7.101931e-53 | 5.609340e-50 |
| MsG0180004249.01 | MsG0780041448.01 | 0.821870 | 3.207931e-53 | 2.641959e-50 |
| MsG0180004466.01 | MsG0780041448.01 | 0.806532 | 7.857424e-50 | 4.275092e-47 |
| MsG0180004605.01 | MsG0780041448.01 | -0.823461 | 1.368004e-53 | 1.178624e-50 |
| MsG0180004743.01 | MsG0780041448.01 | 0.804617 | 1.981799e-49 | 1.026546e-46 |
| MsG0180005153.01 | MsG0780041448.01 | 0.801343 | 9.416475e-49 | 4.486216e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041448.01.T01 | MTR_7g111260 | 97.949 | 585 | 8 | 2 | 7 | 588 | 2 | 585 | 0.0 | 1165 |
| MsG0780041448.01.T01 | MTR_1g085090 | 81.565 | 575 | 97 | 4 | 7 | 577 | 2 | 571 | 0.0 | 964 |
| MsG0780041448.01.T01 | MTR_4g123537 | 56.944 | 576 | 232 | 6 | 8 | 577 | 25 | 590 | 0.0 | 610 |
| MsG0780041448.01.T01 | MTR_3g100980 | 50.000 | 562 | 248 | 9 | 22 | 560 | 14 | 565 | 0.0 | 535 |
| MsG0780041448.01.T01 | MTR_3g100980 | 47.627 | 590 | 248 | 10 | 22 | 560 | 14 | 593 | 1.58e-179 | 520 |
| MsG0780041448.01.T01 | MTR_2g017750 | 43.729 | 590 | 289 | 11 | 8 | 556 | 8 | 595 | 2.45e-158 | 466 |
| MsG0780041448.01.T01 | MTR_8g087780 | 44.178 | 584 | 290 | 10 | 11 | 559 | 2 | 584 | 2.09e-152 | 451 |
| MsG0780041448.01.T01 | MTR_8g087810 | 45.246 | 568 | 280 | 10 | 22 | 559 | 19 | 585 | 1.50e-150 | 446 |
| MsG0780041448.01.T01 | MTR_4g107670 | 41.343 | 566 | 288 | 13 | 22 | 554 | 22 | 576 | 3.53e-141 | 422 |
| MsG0780041448.01.T01 | MTR_2g005130 | 41.045 | 536 | 296 | 9 | 23 | 549 | 39 | 563 | 2.92e-136 | 409 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041448.01.T01 | AT1G59740 | 42.451 | 563 | 308 | 9 | 9 | 558 | 26 | 585 | 1.28e-143 | 428 |
| MsG0780041448.01.T01 | AT1G69850 | 39.153 | 590 | 308 | 9 | 9 | 555 | 4 | 585 | 1.57e-139 | 417 |
| MsG0780041448.01.T01 | AT1G27040 | 41.133 | 547 | 295 | 9 | 22 | 550 | 25 | 562 | 2.59e-135 | 406 |
| MsG0780041448.01.T01 | AT1G27040 | 41.133 | 547 | 295 | 9 | 22 | 550 | 21 | 558 | 4.42e-135 | 405 |
| MsG0780041448.01.T01 | AT1G27040 | 41.588 | 529 | 285 | 8 | 37 | 550 | 11 | 530 | 2.28e-133 | 400 |
| MsG0780041448.01.T01 | AT1G33440 | 43.071 | 534 | 289 | 7 | 24 | 546 | 38 | 567 | 3.41e-130 | 394 |
| MsG0780041448.01.T01 | AT5G62730 | 37.351 | 589 | 307 | 11 | 3 | 552 | 23 | 588 | 1.21e-126 | 385 |
| MsG0780041448.01.T01 | AT5G62730 | 39.074 | 540 | 282 | 9 | 37 | 552 | 13 | 529 | 2.12e-124 | 377 |
| MsG0780041448.01.T01 | AT3G25280 | 39.427 | 558 | 285 | 16 | 5 | 546 | 1 | 521 | 9.08e-122 | 370 |
| MsG0780041448.01.T01 | AT3G54140 | 35.842 | 558 | 338 | 9 | 9 | 549 | 9 | 563 | 7.64e-121 | 369 |
Find 135 sgRNAs with CRISPR-Local
Find 213 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCACCAACCACCCTTC+AGG | 0.414052 | 7:+91026175 | MsG0780041448.01.T01:CDS |
| AAAATGCTTATGAAAATAAG+AGG | 0.426769 | 7:-91026112 | None:intergenic |
| AAACATTCTCAGTACAACAA+GGG | 0.685525 | 7:+91026029 | MsG0780041448.01.T01:CDS |
| AAAGCCAAAATAGGCTTATT+GGG | 0.316965 | 7:-91026312 | None:intergenic |
| AAATCAAGTTGTGTGAAAGG+TGG | 0.639042 | 7:+91024429 | MsG0780041448.01.T01:CDS |
| AAATGCTTATGAAAATAAGA+GGG | 0.637493 | 7:-91026111 | None:intergenic |
| AACTCAAGCTTCTCACTGAA+TGG | 0.442712 | 7:+91023860 | MsG0780041448.01.T01:CDS |
| AAGTCACAAAGATGGAACAG+TGG | 0.681162 | 7:+91025172 | MsG0780041448.01.T01:CDS |
| AATACTCTCACAAACTTCAT+GGG | 0.541870 | 7:+91024116 | MsG0780041448.01.T01:CDS |
| AATAGGCTTATTGGGTTAGA+TGG | 0.254065 | 7:-91026304 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAATGGTCCAGCAAAT+AGG | + | Chr7:91025344-91025363 | MsG0780041448.01.T01:intron | 30.0% | |
| AAACATTCTCAGTACAACAA+GGG | + | Chr7:91026029-91026048 | MsG0780041448.01.T01:CDS | 30.0% | |
| AAAGCCAAAATAGGCTTATT+GGG | - | Chr7:91026315-91026334 | None:intergenic | 30.0% | |
| AACAAAAAACGAAGCTACAA+AGG | - | Chr7:91025653-91025672 | None:intergenic | 30.0% | |
| AATACTCTCACAAACTTCAT+GGG | + | Chr7:91024116-91024135 | MsG0780041448.01.T01:CDS | 30.0% | |
| ACATGGTTATCAATGTCTAT+TGG | + | Chr7:91026445-91026464 | MsG0780041448.01.T01:CDS | 30.0% | |
| ATAGGCATGTTATGTAAGTT+AGG | + | Chr7:91025362-91025381 | MsG0780041448.01.T01:intron | 30.0% | |
| ATCAGTTATATCAATGACAG+TGG | + | Chr7:91026237-91026256 | MsG0780041448.01.T01:CDS | 30.0% | |
| ATTGGAATGAAAGCCAAAAT+AGG | - | Chr7:91026324-91026343 | None:intergenic | 30.0% | |
| ATTTACATCCAAATGCATGT+GGG | + | Chr7:91024406-91024425 | MsG0780041448.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 91023820 | 91027148 | 91023820 | ID=MsG0780041448.01;Name=MsG0780041448.01 |
| Chr7 | mRNA | 91023820 | 91027148 | 91023820 | ID=MsG0780041448.01.T01;Parent=MsG0780041448.01;Name=MsG0780041448.01.T01;_AED=0.24;_eAED=0.24;_QI=0|0.75|0.6|1|1|1|5|368|588 |
| Chr7 | exon | 91023820 | 91023925 | 91023820 | ID=MsG0780041448.01.T01:exon:3924;Parent=MsG0780041448.01.T01 |
| Chr7 | exon | 91024027 | 91024235 | 91024027 | ID=MsG0780041448.01.T01:exon:3925;Parent=MsG0780041448.01.T01 |
| Chr7 | exon | 91024369 | 91024791 | 91024369 | ID=MsG0780041448.01.T01:exon:3926;Parent=MsG0780041448.01.T01 |
| Chr7 | exon | 91025104 | 91025222 | 91025104 | ID=MsG0780041448.01.T01:exon:3927;Parent=MsG0780041448.01.T01 |
| Chr7 | exon | 91025871 | 91027148 | 91025871 | ID=MsG0780041448.01.T01:exon:3928;Parent=MsG0780041448.01.T01 |
| Chr7 | CDS | 91023820 | 91023925 | 91023820 | ID=MsG0780041448.01.T01:cds;Parent=MsG0780041448.01.T01 |
| Chr7 | CDS | 91024027 | 91024235 | 91024027 | ID=MsG0780041448.01.T01:cds;Parent=MsG0780041448.01.T01 |
| Chr7 | CDS | 91024369 | 91024791 | 91024369 | ID=MsG0780041448.01.T01:cds;Parent=MsG0780041448.01.T01 |
| Chr7 | CDS | 91025104 | 91025222 | 91025104 | ID=MsG0780041448.01.T01:cds;Parent=MsG0780041448.01.T01 |
| Chr7 | CDS | 91025871 | 91026780 | 91025871 | ID=MsG0780041448.01.T01:cds;Parent=MsG0780041448.01.T01 |
| Chr7 | three_prime_UTR | 91026781 | 91027148 | 91026781 | ID=MsG0780041448.01.T01:three_prime_utr;Parent=MsG0780041448.01.T01 |
| Gene Sequence |
>MsG0780041448.01.T01 TCAAATCTTATAATACAGGCAGATCAAGAGGAAACTGAAGAACTCAAGCTTCTCACTGAATGGAAGAGAAAGAAAGGTGGATTCAGGGCTTCTATGTTTATTTTTGCATTGGACAATATGGGATTTGTGGCAAACATGGTTAGCTTAGTTTTATATTTTTATGGAGTGATGCACTTTGATCTACCAAGTTCTGCCAATACTCTCACAAACTTCATGGGATCAACTTTCTTGCTTTCCCTTGTTGGTGGCTTCATCTCAGATACTTACTTAAATAGATTCACCACTTGCTTGCTTTTTGGATCTCTAGAAGTTCTGGCTTTGGCATTGGTTACTTTTCAAGCTGCTTCAGATCATTTACATCCAAATGCATGTGGGAAATCAAGTTGTGTGAAAGGTGGAATAGGATTCATGTTTTACACATCACTGACATTGTTGGCATTGGGAATAGGAGGGGTAAGAGGATCTATGACTGCATTTGGTGCTGACCAATTTGAAGAAAAAGATCCAAATGAGGCAAAAGCACTTGCAAGTTTTTTCAATTGGCTTTTGCTTAGTTCAACACTTGGAGCAATTACAGGTGTTACTGGTGTTGTTTGGGTTAGTACACAAAGAGCTTGGCATTGGGGATTTTTCATCATAACTATAGCTTCTTCTATTGGCTTTGTCACACTTGCTCTTGGTAAGCCTTTCTATAGAATCAAAACTCCAGCAGATAGCCCCACAATCAGAATTGCTCAGGTTATTGTTGTGGCATTTAAAAACCGAAAGCTGTCACTGCCAGAGTCACACGAAGAACTATATGAAATAAGTCACAAAGATGGAACAGTGGAGAAAATAGTTCACACAAAACAAATGAGGTTTCTAGATAAAGCAGCAATTCCCCAAGAATACATAATAAAACCCCAACCATGGAAAGTGTGCACAGTGACACAAGTTGAAGAAGTGAAAATCCTAACAAGAATGTTACCAATAGTAGCTAGCACAATCATAATGAACACTTGTTTAGCACAGCTTCAAACATTCTCAGTACAACAAGGGAATGTAATGAACCTTAAACTTGGTTCATTTACTGTTCCAGCATCATCAATTCCTGTCATCCCTCTTATTTTCATAAGCATTTTGGTTCCAATTTATGAACTTTTCTTTGTTCCATTTGCAAGAAAAATCACCAACCACCCTTCAGGAATCACACAACTCCAAAGGGTAGGTGTTGGATTAGTACTATCAGTTATATCAATGACAGTGGCTGGGATAGTAGAAGTCAAAAGAAGGGACCAAAGTAGAAAAGACCCATCTAACCCAATAAGCCTATTTTGGCTTTCATTCCAATATGGCATTTTTGGTATTGCTGATATGTTCACACTTGTGGGACTCTTGGAATTTTTTTATAGGGAATCACCTTCAAGTATGAAATCATTGTCAACATCTTTCACATGGTTATCAATGTCTATTGGTTACTTCCTGAGCACTGTTTTTGTCAATTTGATTAATGTTGTTACCAAAAGGATTACTCCTAGTAAACAAGGTTGGTTACATGGGTTTGATTTGAATCAAAGTAATTTGAACCTGTTCTATTGGTTCCTAGCTATACTTAGTTGTCTTAATTTTTTCAACTACCTTTATTGGGCCTCACGCTATAAGTACAAATCTGAAGACCAAAATTCAAGCCCAATAGATTTGAAGAGTTTGCATGAAATGCCTCTCAAAATGATTGGTGGGACAAAACAAAATTGGGAAGGTAGCATTGGTGGACATACACAAGATTGA |
| Protein sequence |
>MsG0780041448.01.T01 SNLIIQADQEETEELKLLTEWKRKKGGFRASMFIFALDNMGFVANMVSLVLYFYGVMHFDLPSSANTLTNFMGSTFLLSLVGGFISDTYLNRFTTCLLFGSLEVLALALVTFQAASDHLHPNACGKSSCVKGGIGFMFYTSLTLLALGIGGVRGSMTAFGADQFEEKDPNEAKALASFFNWLLLSSTLGAITGVTGVVWVSTQRAWHWGFFIITIASSIGFVTLALGKPFYRIKTPADSPTIRIAQVIVVAFKNRKLSLPESHEELYEISHKDGTVEKIVHTKQMRFLDKAAIPQEYIIKPQPWKVCTVTQVEEVKILTRMLPIVASTIIMNTCLAQLQTFSVQQGNVMNLKLGSFTVPASSIPVIPLIFISILVPIYELFFVPFARKITNHPSGITQLQRVGVGLVLSVISMTVAGIVEVKRRDQSRKDPSNPISLFWLSFQYGIFGIADMFTLVGLLEFFYRESPSSMKSLSTSFTWLSMSIGYFLSTVFVNLINVVTKRITPSKQGWLHGFDLNQSNLNLFYWFLAILSCLNFFNYLYWASRYKYKSEDQNSSPIDLKSLHEMPLKMIGGTKQNWEGSIGGHTQD* |
