AlfalfaGEDB | MsG0580026430.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026430.01

KEGG: -
GO: GO:0016021
PFAM: PF03040

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026430.01.T01	YP_009656679.1	100	57	0	0	1	57	173	229	6.91E-32	119

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026430.01.T01	A6MM48	96.491	57	2	0	1	57	170	226	3.49E-33	115

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026430.01.T01	A0A4P8F2D4	100.000	57	0	0	1	57	173	229	3.30e-32	119

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0580026430.01	0.802326	5.915552e-49	2.889662e-46
MsG0080048068.01	MsG0580026430.01	0.817508	3.180727e-52	2.320865e-49
MsG0080048611.01	MsG0580026430.01	0.806035	1.000122e-49	5.373003e-47
MsG0080048845.01	MsG0580026430.01	0.819609	1.061528e-52	8.207491e-50
MsG0080049078.01	MsG0580026430.01	0.874098	8.803647e-68	3.912276e-64
MsG0080049079.01	MsG0580026430.01	0.877456	6.191762e-69	3.115438e-65
MsG0080049080.01	MsG0580026430.01	0.842791	2.084341e-58	3.195043e-55
MsG0080049130.01	MsG0580026430.01	0.808470	3.049123e-50	1.744936e-47
MsG0180000677.01	MsG0580026430.01	0.812391	4.340377e-51	2.756326e-48
MsG0180000843.01	MsG0580026430.01	0.811966	5.374419e-51	3.373689e-48
MsG0180001085.01	MsG0580026430.01	0.803411	3.531388e-49	1.773355e-46
MsG0180001270.01	MsG0580026430.01	0.801974	6.989770e-49	3.383495e-46
MsG0180001371.01	MsG0580026430.01	0.805999	1.017746e-49	5.462823e-47
MsG0180003198.01	MsG0580026430.01	0.825471	4.603355e-54	4.199634e-51
MsG0180003933.01	MsG0580026430.01	0.832017	1.201527e-55	1.326442e-52
MsG0180003949.01	MsG0580026430.01	0.809502	1.833144e-50	1.078125e-47
MsG0180004188.01	MsG0580026430.01	0.822329	2.510968e-53	2.095198e-50
MsG0180004302.01	MsG0580026430.01	0.809023	2.321834e-50	1.348147e-47
MsG0180004542.01	MsG0580026430.01	0.807452	5.019730e-50	2.797028e-47
MsG0180004733.01	MsG0580026430.01	0.805955	1.039813e-49	5.574807e-47
MsG0180004735.01	MsG0580026430.01	0.808092	3.670806e-50	2.079868e-47
MsG0180004937.01	MsG0580026430.01	0.820754	5.804070e-53	4.632872e-50
MsG0180004987.01	MsG0580026430.01	0.861712	8.462651e-64	2.416069e-60
MsG0180005119.01	MsG0580026430.01	0.823062	1.695117e-53	1.444230e-50
MsG0180005150.01	MsG0580026430.01	0.806764	7.019192e-50	3.841773e-47
MsG0180005391.01	MsG0580026430.01	0.801670	8.068629e-49	3.875907e-46
MsG0180005568.01	MsG0580026430.01	0.861119	1.282875e-63	3.590253e-60
MsG0180005807.01	MsG0580026430.01	0.802364	5.809490e-49	2.840580e-46
MsG0180005879.01	MsG0580026430.01	0.802801	4.721685e-49	2.334496e-46
MsG0180006223.01	MsG0580026430.01	0.802681	4.998455e-49	2.463676e-46
MsG0280010926.01	MsG0580026430.01	0.837367	5.419081e-57	7.023494e-54
MsG0280010974.01	MsG0580026430.01	0.811669	6.236007e-51	3.883452e-48
MsG0280010975.01	MsG0580026430.01	0.834581	2.758770e-56	3.288120e-53
MsG0280011067.01	MsG0580026430.01	0.812394	4.335135e-51	2.753229e-48
MsG0280011270.01	MsG0580026430.01	0.824744	6.838400e-54	6.111420e-51
MsG0280011411.01	MsG0580026430.01	0.830665	2.584396e-55	2.741082e-52
MsG0380011495.01	MsG0580026430.01	0.800849	1.188299e-48	5.591171e-46
MsG0380012443.01	MsG0580026430.01	0.809896	1.508782e-50	8.966191e-48
MsG0380013177.01	MsG0580026430.01	0.807643	4.572392e-50	2.560303e-47
MsG0380013472.01	MsG0580026430.01	0.830347	3.091833e-55	3.248978e-52
MsG0380013927.01	MsG0580026430.01	0.874697	5.516943e-68	2.507099e-64
MsG0380013930.01	MsG0580026430.01	0.806697	7.253909e-50	3.963313e-47
MsG0380013939.01	MsG0580026430.01	0.820122	8.103906e-53	6.357098e-50
MsG0380013955.01	MsG0580026430.01	0.878476	2.723821e-69	1.424406e-65
MsG0380013958.01	MsG0580026430.01	0.865529	5.526553e-65	1.804036e-61
MsG0380013965.01	MsG0580026430.01	0.810540	1.095502e-50	6.621330e-48
MsG0380013970.01	MsG0580026430.01	0.883140	5.771476e-71	3.615147e-67
MsG0380013979.01	MsG0580026430.01	0.808522	2.971919e-50	1.703038e-47
MsG0380013980.01	MsG0580026430.01	0.810406	1.170939e-50	7.052009e-48
MsG0380013992.01	MsG0580026430.01	0.833230	6.007981e-56	6.876114e-53
MsG0380013996.01	MsG0580026430.01	0.854399	1.263095e-61	2.817075e-58
MsG0380014000.01	MsG0580026430.01	0.828812	7.300691e-55	7.335539e-52
MsG0380014007.01	MsG0580026430.01	0.887615	1.221838e-72	9.150726e-69
MsG0380014012.01	MsG0580026430.01	0.866602	2.529211e-65	8.574456e-62
MsG0380014013.01	MsG0580026430.01	0.838582	2.639185e-57	3.550694e-54
MsG0380014022.01	MsG0580026430.01	0.894791	1.768833e-75	1.785664e-71
MsG0380014037.01	MsG0580026430.01	0.879761	9.568044e-70	5.252099e-66
MsG0380014038.01	MsG0580026430.01	0.819402	1.183655e-52	9.099248e-50
MsG0380014042.01	MsG0580026430.01	0.814547	1.457144e-51	9.806338e-49
MsG0380014053.01	MsG0580026430.01	0.845230	4.622093e-59	7.651238e-56
MsG0380014056.01	MsG0580026430.01	0.800422	1.452295e-48	6.759750e-46
MsG0380014729.01	MsG0580026430.01	0.811328	7.396527e-51	4.564960e-48
MsG0480018844.01	MsG0580026430.01	0.800170	1.633755e-48	7.555519e-46
MsG0480018983.01	MsG0580026430.01	0.823201	1.573616e-53	1.345886e-50
MsG0480019582.01	MsG0580026430.01	0.830302	3.170458e-55	3.327063e-52
MsG0480019592.01	MsG0580026430.01	0.832995	6.874103e-56	7.812736e-53
MsG0480019641.01	MsG0580026430.01	0.803267	3.782681e-49	1.892524e-46
MsG0480019971.01	MsG0580026430.01	0.825430	4.707680e-54	4.289789e-51
MsG0480019974.01	MsG0580026430.01	0.809339	1.986884e-50	1.163504e-47
MsG0480020049.01	MsG0580026430.01	0.854398	1.263866e-61	2.818729e-58
MsG0480020350.01	MsG0580026430.01	0.822891	1.858805e-53	1.575965e-50
MsG0480020408.01	MsG0580026430.01	0.807875	4.082770e-50	2.299927e-47
MsG0480020783.01	MsG0580026430.01	0.800854	1.185311e-48	5.577911e-46
MsG0480021168.01	MsG0580026430.01	0.812466	4.179712e-51	2.659708e-48
MsG0480021294.01	MsG0580026430.01	0.841897	3.596815e-58	5.360889e-55
MsG0480021303.01	MsG0580026430.01	0.838840	2.263959e-57	3.069743e-54
MsG0480021367.01	MsG0580026430.01	0.830534	2.782680e-55	2.939807e-52
MsG0480021420.01	MsG0580026430.01	0.833957	3.955400e-56	4.627134e-53
MsG0480022537.01	MsG0580026430.01	0.801347	9.400246e-49	4.478914e-46
MsG0480022804.01	MsG0580026430.01	0.817638	2.972248e-52	2.176405e-49
MsG0480023166.01	MsG0580026430.01	0.803326	3.676514e-49	1.842174e-46
MsG0480023216.01	MsG0580026430.01	0.822392	2.427652e-53	2.029439e-50
MsG0480023351.01	MsG0580026430.01	0.800340	1.509297e-48	7.010374e-46
MsG0480023371.01	MsG0580026430.01	0.818752	1.663419e-52	1.255972e-49
MsG0480023395.01	MsG0580026430.01	0.809244	2.082812e-50	1.216605e-47
MsG0480023422.01	MsG0580026430.01	0.827167	1.817027e-54	1.740344e-51
MsG0480023505.01	MsG0580026430.01	0.834151	3.537372e-56	4.161423e-53
MsG0480023881.01	MsG0580026430.01	0.824450	8.018658e-54	7.104547e-51
MsG0480023990.01	MsG0580026430.01	0.821236	4.493751e-53	3.635521e-50
MsG0480023992.01	MsG0580026430.01	0.808328	3.268340e-50	1.863497e-47
MsG0580024679.01	MsG0580026430.01	0.802039	6.777182e-49	3.286193e-46
MsG0580024873.01	MsG0580026430.01	0.831556	1.561093e-55	1.700158e-52
MsG0580024982.01	MsG0580026430.01	0.820504	6.623025e-53	5.249919e-50
MsG0580025377.01	MsG0580026430.01	0.801990	6.937229e-49	3.359531e-46
MsG0580025589.01	MsG0580026430.01	0.822985	1.767406e-53	1.502498e-50
MsG0380015496.01	MsG0580026430.01	0.802211	6.246194e-49	3.042031e-46
MsG0380015545.01	MsG0580026430.01	0.817048	4.036022e-52	2.908122e-49
MsG0380015603.01	MsG0580026430.01	0.803149	4.001294e-49	1.995863e-46
MsG0380015898.01	MsG0580026430.01	0.808100	3.655455e-50	2.071593e-47
MsG0380016194.01	MsG0580026430.01	0.805651	1.204496e-49	6.407442e-47
MsG0380016284.01	MsG0580026430.01	0.821962	3.054317e-53	2.521875e-50
MsG0380016452.01	MsG0580026430.01	0.817280	3.578279e-52	2.594532e-49
MsG0380016459.01	MsG0580026430.01	0.829016	6.515949e-55	6.586143e-52
MsG0380016751.01	MsG0580026430.01	0.843354	1.475182e-58	2.302118e-55
MsG0380016762.01	MsG0580026430.01	0.864420	1.231827e-64	3.865430e-61
MsG0380017661.01	MsG0580026430.01	0.813671	2.274970e-51	1.495068e-48
MsG0380017728.01	MsG0580026430.01	0.813382	2.633540e-51	1.717238e-48
MsG0380017976.01	MsG0580026430.01	0.820807	5.641986e-53	4.510358e-50
MsG0380018069.01	MsG0580026430.01	0.814244	1.700714e-51	1.135187e-48
MsG0480018089.01	MsG0580026430.01	0.846896	1.627774e-59	2.843201e-56
MsG0480018297.01	MsG0580026430.01	0.805085	1.582556e-49	8.296990e-47
MsG0480018323.01	MsG0580026430.01	0.810233	1.276265e-50	7.651565e-48
MsG0480018324.01	MsG0580026430.01	0.834478	2.928375e-56	3.479483e-53
MsG0580026430.01	MsG0580027098.01	0.819105	1.382863e-52	1.054386e-49
MsG0580026430.01	MsG0580027254.01	0.825675	4.119062e-54	3.779905e-51
MsG0580026430.01	MsG0580028024.01	0.842962	1.876552e-58	2.892028e-55
MsG0580026430.01	MsG0580028101.01	0.807544	4.799857e-50	2.680780e-47
MsG0580026430.01	MsG0580028335.01	0.831815	1.347819e-55	1.479251e-52
MsG0580026430.01	MsG0580028723.01	0.817687	2.898236e-52	2.125088e-49
MsG0580026430.01	MsG0580028853.01	0.850048	2.184516e-60	4.225211e-57
MsG0580026430.01	MsG0580028925.01	0.818308	2.097753e-52	1.564607e-49
MsG0580026430.01	MsG0580029414.01	0.837032	6.603693e-57	8.472224e-54
MsG0580026430.01	MsG0680032057.01	0.810720	1.001911e-50	6.085185e-48
MsG0580026430.01	MsG0680032515.01	0.804544	2.052764e-49	1.061281e-46
MsG0580026430.01	MsG0680032954.01	0.800231	1.587809e-48	7.354602e-46
MsG0580026430.01	MsG0680033224.01	0.803790	2.946518e-49	1.494165e-46
MsG0580026430.01	MsG0680033966.01	0.814562	1.446111e-51	9.736173e-49
MsG0580026430.01	MsG0680034064.01	0.818306	2.100256e-52	1.566380e-49
MsG0580026430.01	MsG0680035308.01	0.817785	2.753906e-52	2.024772e-49
MsG0580026430.01	MsG0680035731.01	0.806356	8.560267e-50	4.636439e-47
MsG0580026430.01	MsG0780036387.01	0.810244	1.269024e-50	7.610635e-48
MsG0580026430.01	MsG0780037638.01	0.816258	6.067354e-52	4.278311e-49
MsG0580026430.01	MsG0780037705.01	0.865860	4.344727e-65	1.434870e-61
MsG0580026430.01	MsG0780037915.01	0.838212	3.287680e-57	4.373089e-54
MsG0580026430.01	MsG0780037946.01	0.828699	7.775587e-55	7.787368e-52
MsG0580026430.01	MsG0780038571.01	0.833127	6.373116e-56	7.272388e-53
MsG0580026430.01	MsG0780038653.01	0.809633	1.718023e-50	1.014041e-47
MsG0580026430.01	MsG0780038986.01	0.813802	2.129016e-51	1.404122e-48
MsG0580026430.01	MsG0780039233.01	0.810323	1.220230e-50	7.333050e-48
MsG0580026430.01	MsG0780039707.01	0.832721	8.041813e-56	9.065043e-53
MsG0580026430.01	MsG0780039859.01	0.803422	3.512749e-49	1.764536e-46
MsG0580026430.01	MsG0780039975.01	0.853663	2.059697e-61	4.483763e-58
MsG0580026430.01	MsG0780040314.01	0.858551	7.623256e-63	1.952933e-59
MsG0580026430.01	MsG0780040315.01	0.826747	2.289672e-54	2.166234e-51
MsG0580026430.01	MsG0780040343.01	0.809376	1.950938e-50	1.143599e-47
MsG0580026430.01	MsG0780040350.01	0.804422	2.176983e-49	1.121951e-46
MsG0580026430.01	MsG0780040935.01	0.808746	2.662070e-50	1.534355e-47
MsG0580026430.01	MsG0780041004.01	0.825254	5.181752e-54	4.698515e-51
MsG0580026430.01	MsG0880042021.01	0.803019	4.257087e-49	2.116284e-46
MsG0580026430.01	MsG0880042290.01	0.824737	6.865160e-54	6.134020e-51
MsG0580026430.01	MsG0880042931.01	0.831333	1.771311e-55	1.916151e-52
MsG0580026430.01	MsG0880043075.01	0.811176	7.978484e-51	4.904736e-48
MsG0580026430.01	MsG0880043541.01	0.841599	4.309419e-58	6.364319e-55
MsG0580026430.01	MsG0880044312.01	0.802209	6.253736e-49	3.045475e-46
MsG0580026430.01	MsG0880044314.01	0.830012	3.731791e-55	3.883507e-52
MsG0580026430.01	MsG0880044392.01	0.800989	1.112299e-48	5.252558e-46
MsG0580026430.01	MsG0880044558.01	0.802811	4.698959e-49	2.323915e-46
MsG0580026430.01	MsG0880044884.01	0.843482	1.364014e-58	2.137042e-55
MsG0580026430.01	MsG0880045261.01	0.815336	9.739587e-52	6.696298e-49
MsG0580026430.01	MsG0880045283.01	0.848327	6.576033e-60	1.202512e-56
MsG0580026430.01	MsG0880045361.01	0.816170	6.348016e-52	4.465364e-49
MsG0580026430.01	MsG0880045412.01	0.843078	1.747986e-58	2.703636e-55
MsG0580026430.01	MsG0880046764.01	0.804100	2.540768e-49	1.298701e-46
MsG0580026430.01	MsG0880047156.01	0.828971	6.680280e-55	6.743332e-52
MsG0580026430.01	MsG0880047451.01	0.831223	1.885809e-55	2.033359e-52
MsG0580026430.01	MsG0880047605.01	0.808015	3.811548e-50	2.155191e-47
MsG0580026430.01	MsG0880047614.01	0.818095	2.343516e-52	1.737583e-49
MsG0280006913.01	MsG0580026430.01	0.801982	6.962213e-49	3.370922e-46
MsG0280007041.01	MsG0580026430.01	0.857783	1.290191e-62	3.220989e-59
MsG0280007628.01	MsG0580026430.01	0.821506	3.893524e-53	3.173504e-50
MsG0280008227.01	MsG0580026430.01	0.804852	1.770632e-49	9.228071e-47
MsG0280010198.01	MsG0580026430.01	0.833107	6.448563e-56	7.353913e-53
MsG0280010496.01	MsG0580026430.01	0.800694	1.278165e-48	5.990119e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026430.01.T01	ATCG00530	82.456	57	10	0	1	57	173	229	4.69e-29	102

Find 8 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTCTGTCTATAAAGATTT+TGG	0.195951	5:-37129211	MsG0580026430.01.T01:CDS
CAGAATGATCAAATTATATC+TGG	0.400622	5:-37129181	MsG0580026430.01.T01:CDS
CATGGTTGGGAACTAATGAT+TGG	0.443529	5:-37129232	None:intergenic
AAAATTGTATCTAAAATGAC+TGG	0.459050	5:+37129142	None:intergenic
ATCTAAAATGACTGGAAAAG+TGG	0.472631	5:+37129150	None:intergenic
ATGGTTGGGAACTAATGATT+GGG	0.515648	5:-37129231	None:intergenic
GAGATACACGATTTAAATAA+CGG	0.522190	5:+37129104	None:intergenic
TGATAAATCACTACAAGTGA+CGG	0.591823	5:+37129082	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAGATACAATTTTAAAATAC+TGG	-	Chr5:37129151-37129170	MsG0580026430.01.T01:CDS	15.0%
!!!	AAAATTGTATCTAAAATGAC+TGG	+	Chr5:37129144-37129163	None:intergenic	20.0%
!	CAGAATGATCAAATTATATC+TGG	-	Chr5:37129102-37129121	MsG0580026430.01.T01:CDS	25.0%
!	GAGATACACGATTTAAATAA+CGG	+	Chr5:37129182-37129201	None:intergenic	25.0%
	TGATAAATCACTACAAGTGA+CGG	+	Chr5:37129204-37129223	None:intergenic	30.0%
!	ATCTAAAATGACTGGAAAAG+TGG	+	Chr5:37129136-37129155	None:intergenic	30.0%
!	GGGTCTGTCTATAAAGATTT+TGG	-	Chr5:37129072-37129091	MsG0580026430.01.T01:CDS	35.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	37129066	37129239	37129066	ID=MsG0580026430.01;Name=MsG0580026430.01
Chr5	mRNA	37129066	37129239	37129066	ID=MsG0580026430.01.T01;Parent=MsG0580026430.01;Name=MsG0580026430.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|57
Chr5	exon	37129066	37129239	37129066	ID=MsG0580026430.01.T01:exon:7192;Parent=MsG0580026430.01.T01
Chr5	CDS	37129066	37129239	37129066	ID=MsG0580026430.01.T01:cds;Parent=MsG0580026430.01.T01
Chr5	mRNA	37129152	37129199	37129152	ID=MsG0580026430.01.T02;Parent=MsG0580026430.01;Name=MsG0580026430.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|15
Chr5	exon	37129152	37129199	37129152	ID=MsG0580026430.01.T02:exon:7193;Parent=MsG0580026430.01.T02
Chr5	CDS	37129152	37129199	37129152	ID=MsG0580026430.01.T02:cds;Parent=MsG0580026430.01.T02
Chr5	mRNA	37129066	37129077	37129066	ID=MsG0580026430.01.T03;Parent=MsG0580026430.01;Name=MsG0580026430.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|3
Chr5	exon	37129066	37129077	37129066	ID=MsG0580026430.01.T03:exon:7194;Parent=MsG0580026430.01.T03
Chr5	CDS	37129066	37129077	37129066	ID=MsG0580026430.01.T03:cds;Parent=MsG0580026430.01.T03

Gene Sequence	>MsG0580026430.01.T01 ATGATTGGGTCTGTCTATAAAGATTTTGGATTTACTCAGAATGATCAAATTATATCTGGTCTTGTTTCCACTTTTCCAGTCATTTTAGATACAATTTTAAAATACTGGATTTTCCGTTATTTAAATCGTGTATCTCCGTCACTTGTAGTGATTTATCATTCAATGAATGACTGA >MsG0580026430.01.T03 ATGAATGACTGA >MsG0580026430.01.T02 ATGATCAAATTATATCTGGTCTTGTTTCCACTTTTCCAGTCATTTTAG
Protein sequence	>MsG0580026430.01.T01 MIGSVYKDFGFTQNDQIISGLVSTFPVILDTILKYWIFRYLNRVSPSLVVIYHSMND* >MsG0580026430.01.T03 MND* >MsG0580026430.01.T02 MIKLYLVLFPLFQSF*