AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049080.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049080.01.T01 MTR_4g051220 83.007 153 21 2 1 149 1 152 8.76e-82 240
MsG0080049080.01.T01 MTR_0002s0590 84.426 122 19 0 1 122 1 122 2.16e-66 206
MsG0080049080.01.T01 MTR_0477s0040 100.000 42 0 0 128 169 1 42 1.17e-23 88.6
MsG0080049080.01.T01 MTR_4g051213 100.000 42 0 0 128 169 1 42 1.17e-23 88.6
MsG0080049080.01.T01 MTR_1g041800 67.857 56 6 1 128 171 1 56 1.33e-17 72.8
MsG0080049080.01.T01 MTR_8g102790 91.892 37 3 0 128 164 1 37 2.05e-17 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049080.01.T01 ATCG00380 93.443 122 8 0 1 122 1 122 6.32e-77 228

Find 23 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATAATTCCGACAACTTC+TGG 0.195705 contig65end:-10180 MsG0080049080.01.T01:CDS
ATGACTAGATCTCGGATAAA+TGG 0.278088 contig65end:-10249 MsG0080049080.01.T01:CDS
AATTATTCGTAATAAGATAT+TGG 0.314059 contig65end:+10194 None:intergenic
CTTAAATACGTTCGTATCGC+TGG 0.342652 contig65end:-11215 MsG0080049080.01.T01:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 contig65end:-11159 MsG0080049080.01.T01:CDS
TACCAGGACTAACTAGTAAA+CGG 0.463029 contig65end:-11358 MsG0080049080.01.T01:CDS
TTTATCCGAGATCTAGTCAT+AGG 0.463215 contig65end:+10252 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 contig65end:+11090 None:intergenic
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 contig65end:+11335 None:intergenic
GATATTGGCTACAATGGTAA+AGG 0.509030 contig65end:+10209 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 contig65end:-11194 MsG0080049080.01.T01:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 contig65end:+11091 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 contig65end:+11356 None:intergenic
TAATAAGATATTGGCTACAA+TGG 0.565840 contig65end:+10203 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 contig65end:+11175 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 contig65end:-11347 MsG0080049080.01.T01:CDS
TGCTACCTATGACTAGATCT+CGG 0.572595 contig65end:-10257 MsG0080049080.01.T01:CDS
GCATTCACTTATTACAGAGA+TGG 0.579208 contig65end:-10147 MsG0080049080.01.T01:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 contig65end:+11098 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 contig65end:-11415 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 contig65end:-11416 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 contig65end:-11203 MsG0080049080.01.T01:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 contig65end:+11075 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCAAATAATTTTATCTTA+CGG + contig65end:11162-11181 None:intergenic 10.0%
!! AATTATTCGTAATAAGATAT+TGG + contig65end:11356-11375 None:intergenic 15.0%
!! ATCAAATAATAGATAGTAAA+TGG - contig65end:10662-10681 MsG0080049080.01.T01:intron 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - contig65end:10663-10682 MsG0080049080.01.T01:intron 15.0%
!!! TAAACAATGAAATTTTAGAA+TGG - contig65end:11265-11284 MsG0080049080.01.T01:CDS 15.0%
!! GTTTATTACAGAAAGAAATT+AGG - contig65end:10856-10875 MsG0080049080.01.T01:intron 20.0%
!! TTCTGTAATAAACAAAATAG+TGG + contig65end:10849-10868 None:intergenic 20.0%
!! TTTGAAAATTAATGAATTGC+TGG - contig65end:10690-10709 MsG0080049080.01.T01:intron 20.0%
! AACATTGATGAACTTGATAA+GGG - contig65end:10939-10958 MsG0080049080.01.T01:intron 25.0%
! ACTAATCCTTTATATTGGAA+TGG + contig65end:10640-10659 None:intergenic 25.0%
! AGTACGAAATAACGTAAAAA+TGG + contig65end:11201-11220 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - contig65end:10667-10686 MsG0080049080.01.T01:intron 25.0%
! GATTGACTAATCCTTTATAT+TGG + contig65end:10645-10664 None:intergenic 25.0%
! TAACATTGATGAACTTGATA+AGG - contig65end:10938-10957 MsG0080049080.01.T01:intron 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + contig65end:10375-10394 None:intergenic 25.0%
! TATTGTACAATTCCTTTGTT+TGG - contig65end:11222-11241 MsG0080049080.01.T01:CDS 25.0%
!! AAATAATTTTATCTTACGGC+AGG + contig65end:11158-11177 None:intergenic 25.0%
!! AACAAAATAGTGGAATAAAG+TGG + contig65end:10839-10858 None:intergenic 25.0%
!! ACAAAATAGTGGAATAAAGT+GGG + contig65end:10838-10857 None:intergenic 25.0%
!! ATTGTACAATTCCTTTGTTT+GGG - contig65end:11223-11242 MsG0080049080.01.T01:CDS 25.0%
!! CAAAAATTGCGTTTTCATTA+TGG - contig65end:10290-10309 MsG0080049080.01.T01:intron 25.0%
!! TAATAAGATATTGGCTACAA+TGG + contig65end:11347-11366 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - contig65end:10410-10429 MsG0080049080.01.T01:intron 25.0%
!!! TTAAGAATAATTTTGATCCG+AGG - contig65end:10761-10780 MsG0080049080.01.T01:intron 25.0%
ACAATTACTTGAAATGCGTT+TGG - contig65end:10388-10407 MsG0080049080.01.T01:intron 30.0%
AGCAAAAAAAACTTACTTCG+AGG - contig65end:11132-11151 MsG0080049080.01.T01:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - contig65end:10533-10552 MsG0080049080.01.T01:intron 30.0%
CATTAACTAAAACATGTCTG+TGG + contig65end:10475-10494 None:intergenic 30.0%
CTTGATCCATTCCAATATAA+AGG - contig65end:10631-10650 MsG0080049080.01.T01:intron 30.0%
TAATCATTATGTAGGAGAGA+TGG + contig65end:11045-11064 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - contig65end:10532-10551 MsG0080049080.01.T01:intron 30.0%
TCATTATTCCAACTTTGATG+TGG + contig65end:10892-10911 None:intergenic 30.0%
TTCAAAAAAATACGTCGTCT+GGG - contig65end:10161-10180 MsG0080049080.01.T01:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + contig65end:10533-10552 None:intergenic 30.0%
TTTCAAAAAAATACGTCGTC+TGG - contig65end:10160-10179 MsG0080049080.01.T01:CDS 30.0%
! TTGTACAATTCCTTTGTTTG+GGG - contig65end:11224-11243 MsG0080049080.01.T01:CDS 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - contig65end:10473-10492 MsG0080049080.01.T01:intron 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - contig65end:10409-10428 MsG0080049080.01.T01:intron 30.0%
ATGACTAGATCTCGGATAAA+TGG - contig65end:11298-11317 MsG0080049080.01.T01:CDS 35.0%
CATACATATTCCATTTGCGT+AGG - contig65end:11088-11107 MsG0080049080.01.T01:CDS 35.0%
GCATTCACTTATTACAGAGA+TGG - contig65end:11400-11419 MsG0080049080.01.T01:CDS 35.0%
GTCTGTGGTTAACTAATTGA+CGG + contig65end:10460-10479 None:intergenic 35.0%
TAAAAGAATCCCCCAAACAA+AGG + contig65end:11237-11256 None:intergenic 35.0%
TACCAGGACTAACTAGTAAA+CGG - contig65end:10189-10208 MsG0080049080.01.T01:CDS 35.0%
TAGAGAATCCACATCAAAGT+TGG - contig65end:10881-10900 MsG0080049080.01.T01:intron 35.0%
TCAAAAAAATACGTCGTCTG+GGG - contig65end:10162-10181 MsG0080049080.01.T01:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + contig65end:10459-10478 None:intergenic 35.0%
TTTATCCGAGATCTAGTCAT+AGG + contig65end:11298-11317 None:intergenic 35.0%
! GATATTGGCTACAATGGTAA+AGG + contig65end:11341-11360 None:intergenic 35.0%
! TCTTTTTCTCCAGAAGTTGT+CGG + contig65end:11379-11398 None:intergenic 35.0%
! TGTACAATTCCTTTGTTTGG+GGG - contig65end:11225-11244 MsG0080049080.01.T01:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - contig65end:10415-10434 MsG0080049080.01.T01:intron 35.0%
!!! CGACGTATTTTTTTGAAACG+AGG + contig65end:10158-10177 None:intergenic 35.0%
ACATGAATGGGGAAAATCCT+CGG + contig65end:10781-10800 None:intergenic 40.0%
ACCCACGTCTATACATGAAT+GGG + contig65end:10793-10812 None:intergenic 40.0%
CAAAAAAATACGTCGTCTGG+GGG - contig65end:10163-10182 MsG0080049080.01.T01:CDS 40.0%
CGAATAATTCCGACAACTTC+TGG - contig65end:11367-11386 MsG0080049080.01.T01:CDS 40.0%
CTTAAATACGTTCGTATCGC+TGG - contig65end:10332-10351 MsG0080049080.01.T01:intron 40.0%
GGAATGGATCAAGAGTCAAA+TGG + contig65end:10624-10643 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + contig65end:10619-10638 None:intergenic 40.0%
TACCCACGTCTATACATGAA+TGG + contig65end:10794-10813 None:intergenic 40.0%
TCATGTATAGACGTGGGTAT+AGG - contig65end:10795-10814 MsG0080049080.01.T01:intron 40.0%
TGAGGTTTGCAACGATAACT+TGG + contig65end:10515-10534 None:intergenic 40.0%
TGATGAACTTGATAAGGGTG+CGG - contig65end:10944-10963 MsG0080049080.01.T01:intron 40.0%
TGCTACCTATGACTAGATCT+CGG - contig65end:11290-11309 MsG0080049080.01.T01:CDS 40.0%
! ATAGTGGAATAAAGTGGGCA+AGG + contig65end:10833-10852 None:intergenic 40.0%
! GACAACTTCTGGAGAAAAAG+AGG - contig65end:11378-11397 MsG0080049080.01.T01:CDS 40.0%
! GAGGGGCATAATCATTATGT+AGG + contig65end:11053-11072 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + contig65end:10606-10625 None:intergenic 40.0%
!! GTAGGCTTTTTGTTTACCGA+GGG + contig65end:10987-11006 None:intergenic 40.0%
!! TGTAGGCTTTTTGTTTACCG+AGG + contig65end:10988-11007 None:intergenic 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + contig65end:10452-10471 None:intergenic 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + contig65end:10241-10260 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - contig65end:10200-10219 MsG0080049080.01.T01:CDS 45.0%
ATGCCTCACTATTCACTCGA+GGG + contig65end:11071-11090 None:intergenic 45.0%
ATTATGTGCGCCTACGCAAA+TGG + contig65end:11101-11120 None:intergenic 45.0%
CCCACGTCTATACATGAATG+GGG + contig65end:10792-10811 None:intergenic 45.0%
CCCCATTCATGTATAGACGT+GGG - contig65end:10789-10808 MsG0080049080.01.T01:intron 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + contig65end:10215-10234 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - contig65end:10353-10372 MsG0080049080.01.T01:intron 45.0%
TATGCCTCACTATTCACTCG+AGG + contig65end:11072-11091 None:intergenic 45.0%
TCCCCATTCATGTATAGACG+TGG - contig65end:10788-10807 MsG0080049080.01.T01:intron 45.0%
! TAGCACTGGAACTGCTATGT+AGG + contig65end:11005-11024 None:intergenic 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + contig65end:10605-10624 None:intergenic 45.0%
!!! CTTAATTTAATATTTTAATT+AGG + contig65end:10746-10765 None:intergenic 5.0%
AAGAGAGGGATTCGAACCCT+CGG - contig65end:10968-10987 MsG0080049080.01.T01:intron 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - contig65end:10344-10363 MsG0080049080.01.T01:intron 50.0%
GGCCGTTTACTAGTTAGTCC+TGG + contig65end:10194-10213 None:intergenic 50.0%
TGCCTCACTATTCACTCGAG+GGG + contig65end:11070-11089 None:intergenic 50.0%
! GATAAGGGTGCGGAAAGAGA+GGG - contig65end:10954-10973 MsG0080049080.01.T01:intron 50.0%
! TGATAAGGGTGCGGAAAGAG+AGG - contig65end:10953-10972 MsG0080049080.01.T01:intron 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + contig65end:10604-10623 None:intergenic 50.0%
AGAGATGGCCGAGTGGTTGA+AGG + contig65end:11030-11049 None:intergenic 55.0%
ATGTAGGAGAGATGGCCGAG+TGG + contig65end:11037-11056 None:intergenic 55.0%
GATTGGCAATTCTTCGCGGG+GGG + contig65end:10602-10621 None:intergenic 55.0%
GTGCTACGCCTTCAACCACT+CGG - contig65end:11019-11038 MsG0080049080.01.T01:intron 55.0%
TGCCCCTCGAGTGAATAGTG+AGG - contig65end:11065-11084 MsG0080049080.01.T01:intron 55.0%
! AGTGGTTGAAGGCGTAGCAC+TGG + contig65end:11019-11038 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + contig65end:10603-10622 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - contig65end:10173-10192 MsG0080049080.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
contig65end gene 10137 11432 10137 ID=MsG0080049080.01;Name=MsG0080049080.01
contig65end mRNA 10137 11432 10137 ID=MsG0080049080.01.T01;Parent=MsG0080049080.01;Name=MsG0080049080.01.T01;_AED=0.49;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|171
contig65end exon 11067 11432 11067 ID=MsG0080049080.01.T01:exon:12837;Parent=MsG0080049080.01.T01
contig65end exon 10137 10286 10137 ID=MsG0080049080.01.T01:exon:12836;Parent=MsG0080049080.01.T01
contig65end CDS 11067 11432 11067 ID=MsG0080049080.01.T01:cds;Parent=MsG0080049080.01.T01
contig65end CDS 10137 10286 10137 ID=MsG0080049080.01.T01:cds;Parent=MsG0080049080.01.T01
Gene Sequence

>MsG0080049080.01.T01

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAAATGGATTGCTACCTATGACTAGATCTCGGATAAATGGAAATTTTATTGATAAGACCTTTACCATTGTAGCCAATATCTTATTACGAATAATTCCGACAACTTCTGGAGAAAAAGAGGCATTCACTTATTACAGAGATGGTGCGATTTGA

Protein sequence

>MsG0080049080.01.T01

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRINGLLPMTRSRINGNFIDKTFTIVANILLRIIPTTSGEKEAFTYYRDGAI*