Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016267.01.T01 | XP_003601914.2 | 96.721 | 122 | 0 | 1 | 1 | 118 | 123 | 244 | 6.78E-79 | 242 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016267.01.T01 | O22227 | 38.76 | 129 | 64 | 4 | 1 | 116 | 170 | 296 | 1.58E-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016267.01.T01 | G7J372 | 96.721 | 122 | 0 | 1 | 1 | 118 | 123 | 244 | 3.24e-79 | 242 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048461.01 | MsG0380016267.01 | 0.830399 | 3.001656e-55 | 3.158861e-52 |
| MsG0080048462.01 | MsG0380016267.01 | 0.806184 | 9.303092e-50 | 5.017082e-47 |
| MsG0080048856.01 | MsG0380016267.01 | 0.804826 | 1.792345e-49 | 9.335051e-47 |
| MsG0180000145.01 | MsG0380016267.01 | 0.824356 | 8.436936e-54 | 7.455233e-51 |
| MsG0180000470.01 | MsG0380016267.01 | 0.814588 | 1.427246e-51 | 9.615782e-49 |
| MsG0180002492.01 | MsG0380016267.01 | 0.804248 | 2.366408e-49 | 1.214120e-46 |
| MsG0180004389.01 | MsG0380016267.01 | 0.804605 | 1.993488e-49 | 1.032279e-46 |
| MsG0180005185.01 | MsG0380016267.01 | 0.804724 | 1.882965e-49 | 9.781239e-47 |
| MsG0180005575.01 | MsG0380016267.01 | 0.807054 | 6.095240e-50 | 3.361266e-47 |
| MsG0380011501.01 | MsG0380016267.01 | 0.810611 | 1.057502e-50 | 6.403976e-48 |
| MsG0380012067.01 | MsG0380016267.01 | 0.802085 | 6.630410e-49 | 3.218902e-46 |
| MsG0380013475.01 | MsG0380016267.01 | 0.822478 | 2.318201e-53 | 1.942626e-50 |
| MsG0380014701.01 | MsG0380016267.01 | 0.818154 | 2.272795e-52 | 1.687979e-49 |
| MsG0380015332.01 | MsG0380016267.01 | 0.807768 | 4.300476e-50 | 2.415944e-47 |
| MsG0380015555.01 | MsG0380016267.01 | 0.802828 | 4.662496e-49 | 2.306912e-46 |
| MsG0380016267.01 | MsG0380016517.01 | 0.804509 | 2.088156e-49 | 1.078580e-46 |
| MsG0380016267.01 | MsG0380016970.01 | 0.804356 | 2.247072e-49 | 1.156164e-46 |
| MsG0380016267.01 | MsG0480023172.01 | 0.811282 | 7.567940e-51 | 4.665178e-48 |
| MsG0380016267.01 | MsG0580027320.01 | 0.804413 | 2.186300e-49 | 1.126508e-46 |
| MsG0380016267.01 | MsG0580027650.01 | 0.803559 | 3.289701e-49 | 1.658426e-46 |
| MsG0380016267.01 | MsG0580027925.01 | 0.809713 | 1.651364e-50 | 9.767136e-48 |
| MsG0380016267.01 | MsG0580028436.01 | 0.802094 | 6.602817e-49 | 3.206207e-46 |
| MsG0380016267.01 | MsG0680030674.01 | 0.818395 | 2.004145e-52 | 1.498519e-49 |
| MsG0380016267.01 | MsG0680030881.01 | 0.816618 | 5.038913e-52 | 3.588149e-49 |
| MsG0380016267.01 | MsG0780036477.01 | 0.804097 | 2.544296e-49 | 1.300423e-46 |
| MsG0380016267.01 | MsG0780038603.01 | 0.801057 | 1.077279e-48 | 5.095829e-46 |
| MsG0380016267.01 | MsG0780039170.01 | 0.806143 | 9.490523e-50 | 5.112690e-47 |
| MsG0380016267.01 | MsG0880042520.01 | 0.802226 | 6.204780e-49 | 3.022966e-46 |
| MsG0380016267.01 | MsG0880045111.01 | 0.813342 | 2.686908e-51 | 1.750102e-48 |
| MsG0380016267.01 | MsG0880045639.01 | 0.803053 | 4.187289e-49 | 2.083461e-46 |
| MsG0380016267.01 | MsG0880047473.01 | 0.813120 | 3.006228e-51 | 1.946117e-48 |
| MsG0280006930.01 | MsG0380016267.01 | 0.801481 | 8.821827e-49 | 4.217644e-46 |
| MsG0280007066.01 | MsG0380016267.01 | 0.804727 | 1.880560e-49 | 9.769384e-47 |
| MsG0280007072.01 | MsG0380016267.01 | 0.801294 | 9.636896e-49 | 4.585615e-46 |
| MsG0280007452.01 | MsG0380016267.01 | 0.803504 | 3.377183e-49 | 1.700061e-46 |
| MsG0280007955.01 | MsG0380016267.01 | 0.828736 | 7.615399e-55 | 7.634676e-52 |
| MsG0280008363.01 | MsG0380016267.01 | 0.806103 | 9.675689e-50 | 5.207094e-47 |
| MsG0280008457.01 | MsG0380016267.01 | 0.803035 | 4.224545e-49 | 2.101037e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016267.01.T01 | MTR_3g086740 | 96.721 | 122 | 0 | 1 | 1 | 118 | 123 | 244 | 8.21e-83 | 242 |
| MsG0380016267.01.T01 | MTR_8g089865 | 63.710 | 124 | 37 | 4 | 1 | 117 | 99 | 221 | 4.11e-47 | 151 |
| MsG0380016267.01.T01 | MTR_8g040690 | 40.000 | 115 | 63 | 3 | 4 | 114 | 132 | 244 | 1.62e-24 | 94.0 |
| MsG0380016267.01.T01 | MTR_7g064810 | 44.628 | 121 | 61 | 3 | 1 | 118 | 107 | 224 | 2.45e-24 | 93.2 |
| MsG0380016267.01.T01 | MTR_8g040680 | 39.316 | 117 | 65 | 3 | 4 | 116 | 142 | 256 | 1.36e-23 | 91.7 |
| MsG0380016267.01.T01 | MTR_3g094830 | 37.190 | 121 | 69 | 4 | 1 | 116 | 140 | 258 | 2.86e-22 | 88.2 |
| MsG0380016267.01.T01 | MTR_1g053130 | 41.985 | 131 | 57 | 4 | 1 | 116 | 157 | 283 | 1.10e-21 | 87.0 |
| MsG0380016267.01.T01 | MTR_3g101300 | 39.370 | 127 | 65 | 5 | 1 | 117 | 112 | 236 | 2.99e-21 | 85.1 |
| MsG0380016267.01.T01 | MTR_4g112370 | 34.400 | 125 | 69 | 3 | 1 | 116 | 139 | 259 | 2.09e-19 | 80.9 |
| MsG0380016267.01.T01 | MTR_2g055990 | 34.426 | 122 | 72 | 3 | 1 | 116 | 139 | 258 | 4.45e-18 | 77.0 |
| MsG0380016267.01.T01 | MTR_2g054380 | 34.127 | 126 | 68 | 4 | 1 | 116 | 149 | 269 | 1.61e-16 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016267.01.T01 | AT5G65340 | 57.143 | 126 | 43 | 3 | 1 | 117 | 127 | 250 | 3.63e-42 | 139 |
| MsG0380016267.01.T01 | AT2G22460 | 56.303 | 119 | 48 | 2 | 1 | 117 | 127 | 243 | 4.62e-34 | 118 |
| MsG0380016267.01.T01 | AT1G21050 | 50.420 | 119 | 48 | 3 | 4 | 117 | 133 | 245 | 1.89e-31 | 111 |
| MsG0380016267.01.T01 | AT1G21050 | 50.420 | 119 | 48 | 3 | 4 | 117 | 169 | 281 | 3.46e-31 | 112 |
| MsG0380016267.01.T01 | AT1G76610 | 50.427 | 117 | 49 | 2 | 1 | 117 | 119 | 226 | 8.00e-31 | 109 |
| MsG0380016267.01.T01 | AT4G39610 | 43.200 | 125 | 57 | 4 | 4 | 116 | 141 | 263 | 6.16e-27 | 100 |
| MsG0380016267.01.T01 | AT2G21990 | 42.500 | 120 | 63 | 3 | 1 | 116 | 134 | 251 | 6.83e-27 | 100 |
| MsG0380016267.01.T01 | AT2G37880 | 39.200 | 125 | 66 | 3 | 1 | 117 | 123 | 245 | 4.42e-23 | 90.1 |
| MsG0380016267.01.T01 | AT5G06990 | 37.302 | 126 | 64 | 3 | 4 | 116 | 137 | 260 | 1.35e-22 | 89.4 |
| MsG0380016267.01.T01 | AT5G42680 | 41.803 | 122 | 64 | 6 | 1 | 117 | 118 | 237 | 9.29e-21 | 84.0 |
| MsG0380016267.01.T01 | AT5G42680 | 41.803 | 122 | 64 | 6 | 1 | 117 | 118 | 237 | 9.29e-21 | 84.0 |
| MsG0380016267.01.T01 | AT2G41660 | 38.760 | 129 | 64 | 4 | 1 | 116 | 170 | 296 | 1.61e-19 | 81.6 |
| MsG0380016267.01.T01 | AT3G25640 | 32.824 | 131 | 70 | 5 | 1 | 116 | 139 | 266 | 6.84e-17 | 74.3 |
| MsG0380016267.01.T01 | AT3G25640 | 32.824 | 131 | 70 | 5 | 1 | 116 | 165 | 292 | 1.00e-16 | 73.9 |
| MsG0380016267.01.T01 | AT5G23100 | 32.673 | 101 | 62 | 2 | 20 | 116 | 178 | 276 | 2.61e-14 | 67.4 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGTACTGGTGTTATAAA+TGG | 0.255094 | 3:-81255101 | MsG0380016267.01.T01:CDS |
| TTTGGATTATGTATTGTAAT+GGG | 0.364697 | 3:-81255202 | MsG0380016267.01.T01:CDS |
| CTTTGGATTATGTATTGTAA+TGG | 0.380120 | 3:-81255203 | MsG0380016267.01.T01:CDS |
| TCTTGACCAATGCTTCCTTC+AGG | 0.407088 | 3:+81254972 | None:intergenic |
| GCTTCTAAATCACTTCTTGA+AGG | 0.409476 | 3:+81255149 | None:intergenic |
| CGGTCGGTTTCTGTTGGTAC+TGG | 0.430039 | 3:-81255113 | MsG0380016267.01.T01:CDS |
| TGGGAGGAAAGTCGGGTATT+CGG | 0.443287 | 3:-81255183 | MsG0380016267.01.T01:CDS |
| ATTGTAATGGGAGGAAAGTC+GGG | 0.463638 | 3:-81255190 | MsG0380016267.01.T01:CDS |
| TCTTAGAGCTAACTTTCAAA+GGG | 0.464488 | 3:-81255042 | MsG0380016267.01.T01:CDS |
| ATCTTAGAGCTAACTTTCAA+AGG | 0.465935 | 3:-81255043 | MsG0380016267.01.T01:CDS |
| TATTGTAATGGGAGGAAAGT+CGG | 0.482311 | 3:-81255191 | MsG0380016267.01.T01:CDS |
| ATTGATCCTGAAGGAAGCAT+TGG | 0.506241 | 3:-81254978 | MsG0380016267.01.T01:CDS |
| GCTAACTTTCAAAGGGTTCG+TGG | 0.509016 | 3:-81255035 | MsG0380016267.01.T01:CDS |
| TTGATGCGGTCGGTTTCTGT+TGG | 0.551122 | 3:-81255119 | MsG0380016267.01.T01:CDS |
| GGATTATGTATTGTAATGGG+AGG | 0.573929 | 3:-81255199 | MsG0380016267.01.T01:CDS |
| TAGAAGCACTTAACTTGATG+CGG | 0.577344 | 3:-81255133 | MsG0380016267.01.T01:CDS |
| TTTCATTTGATTGATCCTGA+AGG | 0.587099 | 3:-81254987 | MsG0380016267.01.T01:CDS |
| AGCACTTAACTTGATGCGGT+CGG | 0.611210 | 3:-81255129 | MsG0380016267.01.T01:CDS |
| TTACAATACATAATCCAAAG+AGG | 0.638246 | 3:+81255206 | None:intergenic |
| TTAGCAAAGGAAATGAGAGG+AGG | 0.650662 | 3:-81255287 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGGATTATGTATTGTAAT+GGG | - | Chr3:81255014-81255033 | MsG0380016267.01.T01:CDS | 20.0% |
| ! | CTTTGGATTATGTATTGTAA+TGG | - | Chr3:81255013-81255032 | MsG0380016267.01.T01:CDS | 25.0% |
| ! | TTACAATACATAATCCAAAG+AGG | + | Chr3:81255013-81255032 | None:intergenic | 25.0% |
| ATCTTAGAGCTAACTTTCAA+AGG | - | Chr3:81255173-81255192 | MsG0380016267.01.T01:CDS | 30.0% | |
| TCTTAGAGCTAACTTTCAAA+GGG | - | Chr3:81255174-81255193 | MsG0380016267.01.T01:CDS | 30.0% | |
| TTTCATTTGATTGATCCTGA+AGG | - | Chr3:81255229-81255248 | MsG0380016267.01.T01:CDS | 30.0% | |
| GCTTCTAAATCACTTCTTGA+AGG | + | Chr3:81255070-81255089 | None:intergenic | 35.0% | |
| ! | GGATTATGTATTGTAATGGG+AGG | - | Chr3:81255017-81255036 | MsG0380016267.01.T01:CDS | 35.0% |
| ! | TATTGTAATGGGAGGAAAGT+CGG | - | Chr3:81255025-81255044 | MsG0380016267.01.T01:CDS | 35.0% |
| !! | ACTTGTTTTCAACTCCTCTT+TGG | - | Chr3:81254996-81255015 | MsG0380016267.01.T01:CDS | 35.0% |
| !! | GTTGGTACTGGTGTTATAAA+TGG | - | Chr3:81255115-81255134 | MsG0380016267.01.T01:CDS | 35.0% |
| !! | TAGAAGCACTTAACTTGATG+CGG | - | Chr3:81255083-81255102 | MsG0380016267.01.T01:CDS | 35.0% |
| ! | ATTGATCCTGAAGGAAGCAT+TGG | - | Chr3:81255238-81255257 | MsG0380016267.01.T01:CDS | 40.0% |
| ! | ATTGTAATGGGAGGAAAGTC+GGG | - | Chr3:81255026-81255045 | MsG0380016267.01.T01:CDS | 40.0% |
| GCTAACTTTCAAAGGGTTCG+TGG | - | Chr3:81255181-81255200 | MsG0380016267.01.T01:CDS | 45.0% | |
| TCTTGACCAATGCTTCCTTC+AGG | + | Chr3:81255247-81255266 | None:intergenic | 45.0% | |
| ! | AGCACTTAACTTGATGCGGT+CGG | - | Chr3:81255087-81255106 | MsG0380016267.01.T01:CDS | 45.0% |
| TGGGAGGAAAGTCGGGTATT+CGG | - | Chr3:81255033-81255052 | MsG0380016267.01.T01:CDS | 50.0% | |
| ! | TTGATGCGGTCGGTTTCTGT+TGG | - | Chr3:81255097-81255116 | MsG0380016267.01.T01:CDS | 50.0% |
| ! | CGGTCGGTTTCTGTTGGTAC+TGG | - | Chr3:81255103-81255122 | MsG0380016267.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 81254941 | 81255297 | 81254941 | ID=MsG0380016267.01;Name=MsG0380016267.01 |
| Chr3 | mRNA | 81254941 | 81255297 | 81254941 | ID=MsG0380016267.01.T01;Parent=MsG0380016267.01;Name=MsG0380016267.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|118 |
| Chr3 | exon | 81254941 | 81255297 | 81254941 | ID=MsG0380016267.01.T01:exon:13757;Parent=MsG0380016267.01.T01 |
| Chr3 | CDS | 81254941 | 81255297 | 81254941 | ID=MsG0380016267.01.T01:cds;Parent=MsG0380016267.01.T01 |
| Gene Sequence |
| Protein sequence |
