AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016449.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016449.01.T01 MTR_3g089035 96.032 126 5 0 1 126 1 126 3.70e-88 252
MsG0380016449.01.T01 MTR_3g089005 65.600 125 43 0 1 125 1 125 3.21e-60 181
MsG0380016449.01.T01 MTR_8g089110 61.905 126 41 2 1 125 1 120 9.51e-54 165
MsG0380016449.01.T01 MTR_2g043730 47.619 126 63 2 1 125 1 124 3.51e-39 128
MsG0380016449.01.T01 MTR_8g088830 55.882 102 44 1 25 125 20 121 5.04e-38 125
MsG0380016449.01.T01 MTR_2g043790 47.917 96 50 0 30 125 4 99 2.18e-29 102
MsG0380016449.01.T01 MTR_5g006040 36.752 117 65 3 14 123 7 121 2.32e-21 84.3
MsG0380016449.01.T01 MTR_8g007020 40.984 122 66 4 10 126 1 121 3.08e-21 84.3
MsG0380016449.01.T01 MTR_1g104800 36.842 114 62 4 18 123 9 120 1.13e-19 80.1
MsG0380016449.01.T01 MTR_8g007035 39.344 122 68 4 10 126 1 121 2.96e-19 79.3
MsG0380016449.01.T01 MTR_1g105130 35.780 109 66 3 19 123 11 119 8.54e-19 77.0
MsG0380016449.01.T01 MTR_1g105120 35.780 109 66 3 19 123 11 119 8.54e-19 77.0
MsG0380016449.01.T01 MTR_7g086160 34.167 120 75 3 8 123 2 121 1.80e-18 76.6
MsG0380016449.01.T01 MTR_7g086220 32.143 112 72 3 9 117 2 112 3.08e-18 75.9
MsG0380016449.01.T01 MTR_4g114870 39.394 99 53 2 32 123 190 288 6.16e-18 78.2
MsG0380016449.01.T01 MTR_4g114870 34.351 131 68 4 12 126 1 129 1.28e-15 72.0
MsG0380016449.01.T01 MTR_7g086140 34.167 120 74 4 8 123 2 120 1.34e-17 74.3
MsG0380016449.01.T01 MTR_7g086190 34.167 120 74 4 8 123 2 120 1.34e-17 74.3
MsG0380016449.01.T01 MTR_1g112700 36.134 119 70 4 11 123 9 127 2.07e-17 74.3
MsG0380016449.01.T01 MTR_3g099580 38.393 112 65 3 15 123 8 118 2.93e-17 73.9
MsG0380016449.01.T01 MTR_0334s0010 35.000 120 69 4 10 123 7 123 7.42e-17 73.2
MsG0380016449.01.T01 MTR_3g099980 40.000 115 61 4 17 123 6 120 1.85e-16 72.4
MsG0380016449.01.T01 MTR_7g086100 34.821 112 69 3 15 123 9 119 2.34e-16 72.4
MsG0380016449.01.T01 MTR_7g086280 34.454 119 73 3 10 123 1 119 2.69e-16 71.2
MsG0380016449.01.T01 MTR_2g088990 39.048 105 59 4 23 123 15 118 3.26e-16 72.0
MsG0380016449.01.T01 MTR_7g086230 35.965 114 66 3 17 123 6 119 3.40e-16 70.9
MsG0380016449.01.T01 MTR_6g083240 33.613 119 70 2 15 124 9 127 3.55e-16 70.9
MsG0380016449.01.T01 MTR_6g023760 40.816 98 53 4 30 123 23 119 4.65e-16 70.9
MsG0380016449.01.T01 MTR_3g099680 39.130 115 62 4 17 123 6 120 2.02e-15 69.7
MsG0380016449.01.T01 MTR_3g099570 39.474 114 61 4 17 123 6 118 6.99e-15 67.8
MsG0380016449.01.T01 MTR_4g067200 29.752 121 72 3 16 123 7 127 1.44e-14 66.6
MsG0380016449.01.T01 MTR_4g066110 29.752 121 72 3 16 123 7 127 1.44e-14 66.6
MsG0380016449.01.T01 MTR_6g080660 31.250 112 71 4 15 123 12 120 5.09e-14 65.9
MsG0380016449.01.T01 MTR_7g086090 34.454 119 73 3 10 123 1 119 5.37e-14 66.2
MsG0380016449.01.T01 MTR_6g013200 31.707 123 80 2 7 125 9 131 9.24e-14 64.7
MsG0380016449.01.T01 MTR_6g013420 30.827 133 82 3 3 125 2 134 2.00e-13 63.9
MsG0380016449.01.T01 MTR_4g007250 32.653 98 64 1 30 125 26 123 5.60e-13 62.4
MsG0380016449.01.T01 MTR_4g081100 28.800 125 82 2 9 126 8 132 1.09e-12 62.4
MsG0380016449.01.T01 MTR_1g090420 34.483 116 67 4 15 123 11 124 7.48e-12 60.1
MsG0380016449.01.T01 MTR_2g079030 30.000 120 78 3 10 123 1 120 8.63e-12 61.2
MsG0380016449.01.T01 MTR_8g099220 30.000 110 70 3 20 123 17 125 1.58e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016449.01.T01 AT2G02850 54.264 129 56 1 1 126 1 129 1.83e-47 149
MsG0380016449.01.T01 AT1G17800 40.945 127 67 4 7 126 12 137 2.91e-27 98.6
MsG0380016449.01.T01 AT2G32300 37.705 122 72 3 8 126 2 122 4.57e-23 90.9
MsG0380016449.01.T01 AT2G32300 37.705 122 72 3 8 126 32 152 7.72e-23 90.9
MsG0380016449.01.T01 AT5G26330 33.607 122 76 2 10 126 1 122 9.61e-22 85.5
MsG0380016449.01.T01 AT3G17675 35.246 122 76 2 5 123 4 125 2.36e-21 83.2
MsG0380016449.01.T01 AT5G07475 38.739 111 61 4 19 123 13 122 2.63e-20 82.0
MsG0380016449.01.T01 AT3G60280 41.237 97 53 3 30 123 21 116 1.76e-19 80.5
MsG0380016449.01.T01 AT3G27200 36.364 121 72 4 10 126 3 122 2.26e-19 79.3
MsG0380016449.01.T01 AT2G44790 40.741 108 54 5 24 126 23 125 1.42e-17 75.1
MsG0380016449.01.T01 AT3G60270 37.113 97 57 3 30 123 23 118 5.05e-17 73.6
MsG0380016449.01.T01 AT2G25060 33.613 119 71 3 16 126 14 132 2.16e-16 71.6
MsG0380016449.01.T01 AT4G31840 29.921 127 81 3 8 126 2 128 7.02e-16 70.1
MsG0380016449.01.T01 AT2G31050 33.043 115 72 3 17 126 12 126 3.93e-15 68.6
MsG0380016449.01.T01 AT2G26720 28.319 113 77 2 18 126 14 126 1.10e-14 67.8
MsG0380016449.01.T01 AT1G72230 36.207 116 68 4 11 123 3 115 1.65e-14 66.6
MsG0380016449.01.T01 AT3G20570 30.952 126 80 3 4 123 2 126 4.13e-13 63.5
MsG0380016449.01.T01 AT5G20230 34.000 100 60 2 33 126 25 124 8.51e-13 62.4
MsG0380016449.01.T01 AT4G30590 29.365 126 78 3 12 126 4 129 1.49e-12 61.6
MsG0380016449.01.T01 AT1G22480 37.931 116 63 5 12 123 1 111 1.64e-12 61.2
MsG0380016449.01.T01 AT2G27035 32.500 120 60 6 16 123 12 122 1.26e-11 58.9
MsG0380016449.01.T01 AT5G15350 29.661 118 75 3 14 123 6 123 2.41e-11 58.2
MsG0380016449.01.T01 AT3G18590 28.926 121 73 6 13 123 7 124 3.54e-11 58.2

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCTGGTCACTGCCAATC+TGG 0.329505 3:-83655383 MsG0380016449.01.T01:CDS
GTCCTCTTGATAGCCTGATT+TGG 0.349417 3:+83655426 None:intergenic
AAAACTGATACTTGGCCTAA+TGG 0.367231 3:-83655754 MsG0380016449.01.T01:CDS
GGTGCTAAAGTGTTCAGTTC+AGG 0.375248 3:-83655455 MsG0380016449.01.T01:CDS
AGAGGACTAAACTGCATAGA+TGG 0.414690 3:+83655349 None:intergenic
TCTTACACCGTTGGAGGACC+TGG 0.442628 3:-83655790 MsG0380016449.01.T01:CDS
GTTCAGGGAATGACCAAATC+AGG 0.451771 3:-83655439 MsG0380016449.01.T01:CDS
CACTTTCAGCCAGAAACAAA+AGG 0.476546 3:+83655824 None:intergenic
TACTTCATATGCAGCTATCC+TGG 0.480047 3:-83655398 MsG0380016449.01.T01:CDS
GAGAATATGACTGAGGGAAG+AGG 0.490305 3:-83655889 None:intergenic
ATCCTGGTCACTGCCAATCT+GGG 0.503016 3:-83655382 MsG0380016449.01.T01:CDS
GTGCTAAAGTGTTCAGTTCA+GGG 0.512300 3:-83655454 MsG0380016449.01.T01:CDS
ATCCCAGATTGGCAGTGACC+AGG 0.530524 3:+83655380 None:intergenic
GGACATACAAAACTGATACT+TGG 0.540585 3:-83655762 MsG0380016449.01.T01:CDS
TGGAAACCTTCATCCCAGAT+TGG 0.543764 3:+83655369 None:intergenic
TCACTGCCAATCTGGGATGA+AGG 0.545313 3:-83655375 MsG0380016449.01.T01:CDS
GCAGCATCTTACACCGTTGG+AGG 0.574303 3:-83655796 MsG0380016449.01.T01:CDS
GTGGTTGCCGTGGACAAAAG+TGG 0.576000 3:-83655503 MsG0380016449.01.T01:CDS
CTGAACACTTTAGCACCTCC+CGG 0.578857 3:+83655461 None:intergenic
AATGCAGCATCTTACACCGT+TGG 0.578987 3:-83655799 MsG0380016449.01.T01:CDS
CACACACAATGTGGTTGCCG+TGG 0.585202 3:-83655513 MsG0380016449.01.T01:CDS
AATAGTGAGAATATGACTGA+GGG 0.586297 3:-83655895 None:intergenic
CTTACACCGTTGGAGGACCT+GGG 0.588429 3:-83655789 MsG0380016449.01.T01:CDS
AAATAGTGAGAATATGACTG+AGG 0.592419 3:-83655896 None:intergenic
TACAACAACTGTAAGACACC+GGG 0.625426 3:-83655479 MsG0380016449.01.T01:CDS
TTACACCGTTGGAGGACCTG+GGG 0.632787 3:-83655788 MsG0380016449.01.T01:CDS
CACGGCAACCACATTGTGTG+TGG 0.635175 3:+83655514 None:intergenic
GACCAAATCAGGCTATCAAG+AGG 0.635565 3:-83655428 MsG0380016449.01.T01:CDS
AGGCAGTGCATCTATGAACA+TGG 0.647845 3:-83655869 MsG0380016449.01.T01:CDS
TGATTCAACCACACACAATG+TGG 0.661122 3:-83655522 MsG0380016449.01.T01:CDS
ATACAACAACTGTAAGACAC+CGG 0.661265 3:-83655480 MsG0380016449.01.T01:CDS
TACACCGTTGGAGGACCTGG+GGG 0.694377 3:-83655787 MsG0380016449.01.T01:CDS
AACAACTGTAAGACACCGGG+AGG 0.711932 3:-83655476 MsG0380016449.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTGAGAAATATTGTTT+TGG + Chr3:83655616-83655635 None:intergenic 15.0%
!!! TCAATTTTACAAAATTGTTT+TGG - Chr3:83655628-83655647 MsG0380016449.01.T01:intron 15.0%
!! AATTTAATTACCGTACAATT+GGG - Chr3:83655577-83655596 MsG0380016449.01.T01:intron 20.0%
!! ACTTTAATTATATGTGCATA+TGG - Chr3:83655667-83655686 MsG0380016449.01.T01:intron 20.0%
!! TAATTTAATTACCGTACAAT+TGG - Chr3:83655576-83655595 MsG0380016449.01.T01:intron 20.0%
!! AATGGAAAAAAGTTTAAAGC+TGG - Chr3:83655502-83655521 MsG0380016449.01.T01:CDS 25.0%
!!! GCTTTAAACTTTTTTCCATT+AGG + Chr3:83655502-83655521 None:intergenic 25.0%
ATATGGTAATTGATTGTTGC+AGG - Chr3:83655684-83655703 MsG0380016449.01.T01:intron 30.0%
CATCAAATATCCCAATTGTA+CGG + Chr3:83655590-83655609 None:intergenic 30.0%
AAAACTGATACTTGGCCTAA+TGG - Chr3:83655484-83655503 MsG0380016449.01.T01:CDS 35.0%
ATACAACAACTGTAAGACAC+CGG - Chr3:83655758-83655777 MsG0380016449.01.T01:CDS 35.0%
GGACATACAAAACTGATACT+TGG - Chr3:83655476-83655495 MsG0380016449.01.T01:CDS 35.0%
CACTTTCAGCCAGAAACAAA+AGG + Chr3:83655417-83655436 None:intergenic 40.0%
TACAACAACTGTAAGACACC+GGG - Chr3:83655759-83655778 MsG0380016449.01.T01:CDS 40.0%
TACTTCATATGCAGCTATCC+TGG - Chr3:83655840-83655859 MsG0380016449.01.T01:CDS 40.0%
TGATTCAACCACACACAATG+TGG - Chr3:83655716-83655735 MsG0380016449.01.T01:intron 40.0%
! GTGCTAAAGTGTTCAGTTCA+GGG - Chr3:83655784-83655803 MsG0380016449.01.T01:CDS 40.0%
!! GTTGTATCCACTTTTGTCCA+CGG + Chr3:83655745-83655764 None:intergenic 40.0%
AATGCAGCATCTTACACCGT+TGG - Chr3:83655439-83655458 MsG0380016449.01.T01:CDS 45.0%
AGGCAGTGCATCTATGAACA+TGG - Chr3:83655369-83655388 MsG0380016449.01.T01:CDS 45.0%
GACCAAATCAGGCTATCAAG+AGG - Chr3:83655810-83655829 MsG0380016449.01.T01:CDS 45.0%
GTTCAGGGAATGACCAAATC+AGG - Chr3:83655799-83655818 MsG0380016449.01.T01:CDS 45.0%
TGGAAACCTTCATCCCAGAT+TGG + Chr3:83655872-83655891 None:intergenic 45.0%
! GGTGCTAAAGTGTTCAGTTC+AGG - Chr3:83655783-83655802 MsG0380016449.01.T01:CDS 45.0%
! GTCCTCTTGATAGCCTGATT+TGG + Chr3:83655815-83655834 None:intergenic 45.0%
!! ACTGCTGTGCCTTTTGTTTC+TGG - Chr3:83655405-83655424 MsG0380016449.01.T01:CDS 45.0%
AACAACTGTAAGACACCGGG+AGG - Chr3:83655762-83655781 MsG0380016449.01.T01:CDS 50.0%
ATCCTGGTCACTGCCAATCT+GGG - Chr3:83655856-83655875 MsG0380016449.01.T01:CDS 50.0%
CTGAACACTTTAGCACCTCC+CGG + Chr3:83655780-83655799 None:intergenic 50.0%
TATCCTGGTCACTGCCAATC+TGG - Chr3:83655855-83655874 MsG0380016449.01.T01:CDS 50.0%
! GTTTTGTATGTCCATCCCCC+AGG + Chr3:83655469-83655488 None:intergenic 50.0%
! TCACTGCCAATCTGGGATGA+AGG - Chr3:83655863-83655882 MsG0380016449.01.T01:CDS 50.0%
ATCCCAGATTGGCAGTGACC+AGG + Chr3:83655861-83655880 None:intergenic 55.0%
CACACACAATGTGGTTGCCG+TGG - Chr3:83655725-83655744 MsG0380016449.01.T01:CDS 55.0%
CACGGCAACCACATTGTGTG+TGG + Chr3:83655727-83655746 None:intergenic 55.0%
CTTACACCGTTGGAGGACCT+GGG - Chr3:83655449-83655468 MsG0380016449.01.T01:CDS 55.0%
GCAGCATCTTACACCGTTGG+AGG - Chr3:83655442-83655461 MsG0380016449.01.T01:CDS 55.0%
GTGGTTGCCGTGGACAAAAG+TGG - Chr3:83655735-83655754 MsG0380016449.01.T01:CDS 55.0%
TCTTACACCGTTGGAGGACC+TGG - Chr3:83655448-83655467 MsG0380016449.01.T01:CDS 55.0%
TTACACCGTTGGAGGACCTG+GGG - Chr3:83655450-83655469 MsG0380016449.01.T01:CDS 55.0%
TACACCGTTGGAGGACCTGG+GGG - Chr3:83655451-83655470 MsG0380016449.01.T01:CDS 60.0%
CCATCCCCCAGGTCCTCCAA+CGG + Chr3:83655458-83655477 None:intergenic 65.0%
CCGTTGGAGGACCTGGGGGA+TGG - Chr3:83655455-83655474 MsG0380016449.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 83655355 83655905 83655355 ID=MsG0380016449.01;Name=MsG0380016449.01
Chr3 mRNA 83655355 83655905 83655355 ID=MsG0380016449.01.T01;Parent=MsG0380016449.01;Name=MsG0380016449.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|126
Chr3 exon 83655725 83655905 83655725 ID=MsG0380016449.01.T01:exon:33328;Parent=MsG0380016449.01.T01
Chr3 exon 83655355 83655554 83655355 ID=MsG0380016449.01.T01:exon:33327;Parent=MsG0380016449.01.T01
Chr3 CDS 83655725 83655905 83655725 ID=MsG0380016449.01.T01:cds;Parent=MsG0380016449.01.T01
Chr3 CDS 83655355 83655554 83655355 ID=MsG0380016449.01.T01:cds;Parent=MsG0380016449.01.T01
Gene Sequence

>MsG0380016449.01.T01

ATGACTGAGGGAAGAGGCAGTGCATCTATGAACATGGTCACTGTAATTTCACTGCTGTGCCTTTTGTTTCTGGCTGAAAGTGCTAATGCAGCATCTTACACCGTTGGAGGACCTGGGGGATGGACATACAAAACTGATACTTGGCCTAATGGAAAAAAGTTTAAAGCTGGTGATGTGCTCAGCTTCAACTATGATTCAACCACACACAATGTGGTTGCCGTGGACAAAAGTGGATACAACAACTGTAAGACACCGGGAGGTGCTAAAGTGTTCAGTTCAGGGAATGACCAAATCAGGCTATCAAGAGGACAAAACTACTTCATATGCAGCTATCCTGGTCACTGCCAATCTGGGATGAAGGTTTCCATCTATGCAGTTTAG

Protein sequence

>MsG0380016449.01.T01

MTEGRGSASMNMVTVISLLCLLFLAESANAASYTVGGPGGWTYKTDTWPNGKKFKAGDVLSFNYDSTTHNVVAVDKSGYNNCKTPGGAKVFSSGNDQIRLSRGQNYFICSYPGHCQSGMKVSIYAV*