AlfalfaGEDB | MsG0480019927.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019927.01

KEGG: K11841
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019927.01.T01	XP_003595735.1	58.659	179	26	3	30	161	5	182	2.24E-48	174

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019927.01.T01	G7IGQ8	58.659	179	26	3	30	161	5	182	1.07e-48	174

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048602.01	MsG0480019927.01	0.830140	3.473526e-55	3.628215e-52
MsG0180000146.01	MsG0480019927.01	0.803938	2.745323e-49	1.397519e-46
MsG0180000196.01	MsG0480019927.01	0.802085	6.631327e-49	3.219304e-46
MsG0180000300.01	MsG0480019927.01	0.804108	2.530685e-49	1.293805e-46
MsG0180000555.01	MsG0480019927.01	0.865364	6.230413e-65	2.021653e-61
MsG0180000585.01	MsG0480019927.01	0.803302	3.719988e-49	1.862767e-46
MsG0180000741.01	MsG0480019927.01	0.847050	1.477326e-59	2.593431e-56
MsG0180001566.01	MsG0480019927.01	0.822776	1.977130e-53	1.670869e-50
MsG0180002071.01	MsG0480019927.01	0.848342	6.513525e-60	1.191640e-56
MsG0180002072.01	MsG0480019927.01	0.816178	6.320766e-52	4.447148e-49
MsG0180002894.01	MsG0480019927.01	0.804826	1.792984e-49	9.338161e-47
MsG0180002952.01	MsG0480019927.01	0.803624	3.189433e-49	1.610447e-46
MsG0180003167.01	MsG0480019927.01	0.805749	1.148680e-49	6.126280e-47
MsG0180004372.01	MsG0480019927.01	0.822502	2.288800e-53	1.919275e-50
MsG0180004797.01	MsG0480019927.01	0.803037	4.219577e-49	2.098689e-46
MsG0180005228.01	MsG0480019927.01	0.824856	6.433558e-54	5.768003e-51
MsG0180005559.01	MsG0480019927.01	0.800151	1.648994e-48	7.622230e-46
MsG0180006186.01	MsG0480019927.01	0.802258	6.110846e-49	2.979683e-46
MsG0180006207.01	MsG0480019927.01	0.807953	3.929082e-50	2.217988e-47
MsG0280010816.01	MsG0480019927.01	0.817835	2.683809e-52	1.975899e-49
MsG0280010932.01	MsG0480019927.01	0.826396	2.775312e-54	2.599676e-51
MsG0280011075.01	MsG0480019927.01	0.802329	5.907435e-49	2.885871e-46
MsG0280011085.01	MsG0480019927.01	0.831912	1.275740e-55	1.404128e-52
MsG0280011285.01	MsG0480019927.01	0.803714	3.056026e-49	1.546592e-46
MsG0280011334.01	MsG0480019927.01	0.805861	1.088247e-49	5.820528e-47
MsG0480019927.01	MsG0480021784.01	0.800600	1.335541e-48	6.244296e-46
MsG0480019927.01	MsG0480021900.01	0.818108	2.328078e-52	1.726802e-49
MsG0480019927.01	MsG0480022261.01	0.800002	1.767794e-48	8.140610e-46
MsG0480019927.01	MsG0480022318.01	0.828073	1.101060e-54	1.082845e-51
MsG0480019927.01	MsG0480022552.01	0.824278	8.802800e-54	7.760933e-51
MsG0480019927.01	MsG0480022597.01	0.806738	7.111286e-50	3.889554e-47
MsG0480019927.01	MsG0480022598.01	0.824583	7.462174e-54	6.637825e-51
MsG0480019927.01	MsG0480022719.01	0.813176	2.922307e-51	1.894689e-48
MsG0480019927.01	MsG0480023460.01	0.804767	1.844762e-49	9.592973e-47
MsG0480019927.01	MsG0580024157.01	0.813711	2.228771e-51	1.466369e-48
MsG0480019927.01	MsG0580024682.01	0.821815	3.304326e-53	2.717108e-50
MsG0480019927.01	MsG0580025907.01	0.828381	9.277521e-55	9.206525e-52
MsG0480019927.01	MsG0680030684.01	0.802207	6.258492e-49	3.047640e-46
MsG0480019927.01	MsG0680033302.01	0.810124	1.347151e-50	8.053805e-48
MsG0480019927.01	MsG0680034067.01	0.808864	2.512119e-50	1.452454e-47
MsG0480019927.01	MsG0680034757.01	0.808197	3.485544e-50	1.980448e-47
MsG0480019927.01	MsG0680035764.01	0.802425	5.644235e-49	2.764040e-46
MsG0480019927.01	MsG0780037975.01	0.822429	2.380022e-53	1.991682e-50
MsG0480019927.01	MsG0780039655.01	0.831682	1.453173e-55	1.588510e-52
MsG0480019927.01	MsG0780041033.01	0.814676	1.364702e-51	9.215939e-49
MsG0480019927.01	MsG0880043921.01	0.817937	2.545146e-52	1.878996e-49
MsG0480019927.01	MsG0880045429.01	0.812983	3.221002e-51	2.077578e-48
MsG0480019927.01	MsG0880045448.01	0.830158	3.436841e-55	3.591895e-52
MsG0480019927.01	MsG0880045936.01	0.823876	1.093643e-53	9.534436e-51
MsG0380015436.01	MsG0480019927.01	0.817243	3.648430e-52	2.642759e-49
MsG0380017045.01	MsG0480019927.01	0.819084	1.398288e-52	1.065493e-49
MsG0380017077.01	MsG0480019927.01	0.809011	2.336186e-50	1.356030e-47
MsG0380017139.01	MsG0480019927.01	0.834135	3.571347e-56	4.199422e-53
MsG0380017232.01	MsG0480019927.01	0.804800	1.815675e-49	9.449882e-47
MsG0280007087.01	MsG0480019927.01	0.825549	4.412523e-54	4.034531e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019927.01.T01	MTR_2g060050	58.659	179	26	3	30	161	5	182	2.72e-52	174

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTGATTCTCAGAAATTTAA+AGG	0.150466	4:+29928342	MsG0480019927.01.T01:CDS
TGGGAGCAGGTGTTACTATT+TGG	0.239238	4:+29928153	MsG0480019927.01.T01:CDS
TTTGGTGCTTTCGGTGAATT+TGG	0.281536	4:-29928306	None:intergenic
CCAGTTTACGAATTTGAGTT+TGG	0.307610	4:+29928528	MsG0480019927.01.T01:intron
ATTTAACCAAGACTTGAAAA+AGG	0.314327	4:+29928621	MsG0480019927.01.T01:CDS
ATAAATTCCCAGAGTTAATA+AGG	0.321255	4:-29928691	None:intergenic
TCACGGAGAGGAGCACTATT+TGG	0.352711	4:-29928324	None:intergenic
AAGAAGAATCAGCTGCAATT+TGG	0.368463	4:+29928248	MsG0480019927.01.T01:CDS
GGAGCACTATTTGGTGCTTT+CGG	0.387557	4:-29928315	None:intergenic
AGCTGATTCTTCTTCTCCAC+AGG	0.405590	4:-29928239	None:intergenic
TGCATAACAGCATGCCCATT+AGG	0.406025	4:-29928646	None:intergenic
GTGCTTTCGGTGAATTTGGC+AGG	0.453991	4:-29928302	None:intergenic
AGATGAAACCAGATCATTGT+GGG	0.541732	4:+29928188	MsG0480019927.01.T01:CDS
TTTGAGTTTGGATGCTTCCG+AGG	0.542094	4:+29928540	MsG0480019927.01.T01:CDS
CAGAAATTTAAAGGAGTGAA+CGG	0.548010	4:+29928351	MsG0480019927.01.T01:CDS
CTTTAAATTTCTGAGAATCA+CGG	0.550479	4:-29928341	None:intergenic
GTTCTTCAAACTTTCAGCCT+CGG	0.573342	4:-29928557	None:intergenic
TCCCAGAGTTAATAAGGCCG+AGG	0.578821	4:-29928685	None:intergenic
TGCGAGAAATGAAAAGCCTG+TGG	0.616964	4:+29928223	MsG0480019927.01.T01:CDS
GCATGCTGTTATGCATGCTA+AGG	0.640605	4:+29928654	MsG0480019927.01.T01:CDS
AAGATGAAACCAGATCATTG+TGG	0.658086	4:+29928187	MsG0480019927.01.T01:CDS
ATGCTAAGGATCTTCAGCCT+CGG	0.666491	4:+29928668	MsG0480019927.01.T01:CDS
AATTTCTGAGAATCACGGAG+AGG	0.675867	4:-29928336	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAAAATGTTGTCAATTG+CGG	-	Chr4:29928094-29928113	None:intergenic	20.0%
!	AAAAAAATGTTGTCAATTGC+GGG	-	Chr4:29928093-29928112	None:intergenic	25.0%
!	AACAGAACTATAAAAGAGAA+TGG	+	Chr4:29928398-29928417	MsG0480019927.01.T01:intron	25.0%
!	ATTTAACCAAGACTTGAAAA+AGG	+	Chr4:29928621-29928640	MsG0480019927.01.T01:CDS	25.0%
!	TTGAAGAACGTGTTTAAAAA+TGG	+	Chr4:29928571-29928590	MsG0480019927.01.T01:CDS	25.0%
!!	CTTTAAATTTCTGAGAATCA+CGG	-	Chr4:29928344-29928363	None:intergenic	25.0%
!!!	TTTTATAGTTCTGTTACTTG+AGG	-	Chr4:29928393-29928412	None:intergenic	25.0%
	AACGTGTTTAAAAATGGTAG+TGG	+	Chr4:29928577-29928596	MsG0480019927.01.T01:CDS	30.0%
	CAGAAATTTAAAGGAGTGAA+CGG	+	Chr4:29928351-29928370	MsG0480019927.01.T01:CDS	30.0%
	CGTGATTCTCAGAAATTTAA+AGG	+	Chr4:29928342-29928361	MsG0480019927.01.T01:CDS	30.0%
	GTTACACCAATTGATGAAAA+TGG	+	Chr4:29928496-29928515	MsG0480019927.01.T01:intron	30.0%
!!!	AGAAAATGCGACTGTTTTTT+GGG	+	Chr4:29928128-29928147	MsG0480019927.01.T01:CDS	30.0%
!!!	TAGAAAATGCGACTGTTTTT+TGG	+	Chr4:29928127-29928146	MsG0480019927.01.T01:CDS	30.0%
	AAGAAGAATCAGCTGCAATT+TGG	+	Chr4:29928248-29928267	MsG0480019927.01.T01:CDS	35.0%
	AAGATGAAACCAGATCATTG+TGG	+	Chr4:29928187-29928206	MsG0480019927.01.T01:CDS	35.0%
	AGATGAAACCAGATCATTGT+GGG	+	Chr4:29928188-29928207	MsG0480019927.01.T01:CDS	35.0%
	AGCACTACTAATTTCTCCAT+CGG	+	Chr4:29928422-29928441	MsG0480019927.01.T01:intron	35.0%
	CCAAACTCAAATTCGTAAAC+TGG	-	Chr4:29928531-29928550	None:intergenic	35.0%
	TTTGCACTAGTGATACTTGA+GGG	-	Chr4:29928449-29928468	None:intergenic	35.0%
!	ACTTTTTACCCACAATGATC+TGG	-	Chr4:29928199-29928218	None:intergenic	35.0%
!	CCAGTTTACGAATTTGAGTT+TGG	+	Chr4:29928528-29928547	MsG0480019927.01.T01:intron	35.0%
!	GCAGTACCATTTTCATCAAT+TGG	-	Chr4:29928505-29928524	None:intergenic	35.0%
!!	ACTTGAAAAAGGCACCTAAT+GGG	+	Chr4:29928632-29928651	MsG0480019927.01.T01:CDS	35.0%
!!	GTGTTTAAAAATGGTAGTGG+TGG	+	Chr4:29928580-29928599	MsG0480019927.01.T01:CDS	35.0%
	GTTCTTCAAACTTTCAGCCT+CGG	-	Chr4:29928560-29928579	None:intergenic	40.0%
	GTTTGCACTAGTGATACTTG+AGG	-	Chr4:29928450-29928469	None:intergenic	40.0%
!	AATTTCTGAGAATCACGGAG+AGG	-	Chr4:29928339-29928358	None:intergenic	40.0%
!!	GACTTGAAAAAGGCACCTAA+TGG	+	Chr4:29928631-29928650	MsG0480019927.01.T01:CDS	40.0%
!!	TTTGGTGCTTTCGGTGAATT+TGG	-	Chr4:29928309-29928328	None:intergenic	40.0%
!!!	ATGCGACTGTTTTTTGGGTT+TGG	+	Chr4:29928133-29928152	MsG0480019927.01.T01:CDS	40.0%
!!!	TAGGTGCCTTTTTCAAGTCT+TGG	-	Chr4:29928630-29928649	None:intergenic	40.0%
!!!	TGCGACTGTTTTTTGGGTTT+GGG	+	Chr4:29928134-29928153	MsG0480019927.01.T01:CDS	40.0%
	ATGCTAAGGATCTTCAGCCT+CGG	+	Chr4:29928668-29928687	MsG0480019927.01.T01:CDS	45.0%
	GCATGCTGTTATGCATGCTA+AGG	+	Chr4:29928654-29928673	MsG0480019927.01.T01:CDS	45.0%
	TGCATAACAGCATGCCCATT+AGG	-	Chr4:29928649-29928668	None:intergenic	45.0%
	TGCGAGAAATGAAAAGCCTG+TGG	+	Chr4:29928223-29928242	MsG0480019927.01.T01:CDS	45.0%
!	AGCTGATTCTTCTTCTCCAC+AGG	-	Chr4:29928242-29928261	None:intergenic	45.0%
!	TTTGAGTTTGGATGCTTCCG+AGG	+	Chr4:29928540-29928559	MsG0480019927.01.T01:CDS	45.0%
!!	GGAGCACTATTTGGTGCTTT+CGG	-	Chr4:29928318-29928337	None:intergenic	45.0%
!!	TGGGAGCAGGTGTTACTATT+TGG	+	Chr4:29928153-29928172	MsG0480019927.01.T01:CDS	45.0%
!!!	TGTTTTTTGGGTTTGGGAGC+AGG	+	Chr4:29928140-29928159	MsG0480019927.01.T01:CDS	45.0%
	AGTGATACTTGAGGGACCGA+TGG	-	Chr4:29928441-29928460	None:intergenic	50.0%
	GTGCTTTCGGTGAATTTGGC+AGG	-	Chr4:29928305-29928324	None:intergenic	50.0%
!!	TCACGGAGAGGAGCACTATT+TGG	-	Chr4:29928327-29928346	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	29928072	29928699	29928072	ID=MsG0480019927.01;Name=MsG0480019927.01
Chr4	mRNA	29928072	29928699	29928072	ID=MsG0480019927.01.T01;Parent=MsG0480019927.01;Name=MsG0480019927.01.T01;_AED=0.55;_eAED=0.56;_QI=0\|0\|0\|1\|1\|1\|2\|0\|163
Chr4	exon	29928072	29928395	29928072	ID=MsG0480019927.01.T01:exon:12423;Parent=MsG0480019927.01.T01
Chr4	exon	29928532	29928699	29928532	ID=MsG0480019927.01.T01:exon:12424;Parent=MsG0480019927.01.T01
Chr4	CDS	29928072	29928395	29928072	ID=MsG0480019927.01.T01:cds;Parent=MsG0480019927.01.T01
Chr4	CDS	29928532	29928699	29928532	ID=MsG0480019927.01.T01:cds;Parent=MsG0480019927.01.T01

Gene Sequence

>MsG0480019927.01.T01

ATGCACATTTTCTGCAATCCCGCAATTGACAACATTTTTTTAAATTTTCATATTGTAGAAAATGCGACTGTTTTTTGGGTTTGGGAGCAGGTGTTACTATTTGGCTCATTTACTGAAGATGAAACCAGATCATTGTGGGTAAAAAGTCTTCTGCGAGAAATGAAAAGCCTGTGGAGAAGAAGAATCAGCTGCAATTTGGTTCTCTGTTCTGTTACTGTTGAATCAAGCAACCTGCCAAATTCACCGAAAGCACCAAATAGTGCTCCTCTCCGTGATTCTCAGAAATTTAAAGGAGTGAACGGCGTCAGTGTGAATTTACCTCAATTTACGAATTTGAGTTTGGATGCTTCCGAGGCTGAAAGTTTGAAGAACGTGTTTAAAAATGGTAGTGGTGGCGATTCTTCATCAAAGTTATTTAACCAAGACTTGAAAAAGGCACCTAATGGGCATGCTGTTATGCATGCTAAGGATCTTCAGCCTCGGCCTTATTAA

Protein sequence

>MsG0480019927.01.T01

MHIFCNPAIDNIFLNFHIVENATVFWVWEQVLLFGSFTEDETRSLWVKSLLREMKSLWRRRISCNLVLCSVTVESSNLPNSPKAPNSAPLRDSQKFKGVNGVSVNLPQFTNLSLDASEAESLKNVFKNGSGGDSSSKLFNQDLKKAPNGHAVMHAKDLQPRPY*