AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045936.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045936.01.T01 MTR_4g095058 92.105 190 11 1 3 188 16 205 1.61e-121 356
MsG0880045936.01.T01 MTR_4g095058 92.553 188 10 1 3 186 16 203 4.62e-120 353
MsG0880045936.01.T01 MTR_5g016350 62.176 193 66 4 3 188 16 208 1.24e-63 207
MsG0880045936.01.T01 MTR_5g016370 49.038 208 83 5 3 188 16 222 1.38e-57 191
MsG0880045936.01.T01 MTR_5g016350 56.835 139 53 4 57 188 6 144 8.32e-28 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045936.01.T01 AT5G67320 58.065 124 43 3 3 117 16 139 9.25e-42 149

Find 36 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATTCGGCTTATACTTT+TGG 0.274410 8:+66675265 MsG0880045936.01.T01:CDS
GATGCTAACATTATTCAAAT+TGG 0.297889 8:-66675392 None:intergenic
ATTAGCAAATGCCCCATTGA+TGG 0.333030 8:+66675301 MsG0880045936.01.T01:CDS
CGGTACCAAGTTTCCATCAA+TGG 0.392522 8:-66675314 None:intergenic
TCTTGTGACATTTGTTCAAA+AGG 0.412995 8:+66675345 MsG0880045936.01.T01:CDS
TACCGCAGGTTTCACACATT+CGG 0.417601 8:+66675252 MsG0880045936.01.T01:CDS
GAGAAGTAAAGAATTAGAAA+AGG 0.420779 8:+66675648 MsG0880045936.01.T01:CDS
GGTACCAAGTTTCCATCAAT+GGG 0.424902 8:-66675313 None:intergenic
CTTGTGACATTTGTTCAAAA+GGG 0.440462 8:+66675346 MsG0880045936.01.T01:CDS
GGCAAATTATTAGTGAAAGA+AGG 0.452395 8:+66675608 MsG0880045936.01.T01:CDS
GAGACAAGGAAAGACATCAT+AGG 0.453256 8:+66675698 MsG0880045936.01.T01:CDS
AGCATGAAGTGAAGACTGAC+AGG 0.477378 8:+66675830 MsG0880045936.01.T01:CDS
AGAAAGAATTTGAAAAGGAT+AGG 0.478845 8:+66675722 MsG0880045936.01.T01:CDS
CAAAAGATGTGCATGAACTT+AGG 0.491410 8:+66675587 MsG0880045936.01.T01:CDS
ATGCCCCATTGATGGAAACT+TGG 0.505487 8:+66675309 MsG0880045936.01.T01:CDS
TAGGGAGAAAGAATTTGAAA+AGG 0.512580 8:+66675717 MsG0880045936.01.T01:CDS
AGACAAGGAAAGACATCATA+GGG 0.514191 8:+66675699 MsG0880045936.01.T01:CDS
ACTGTAAAACATGAAGTGAA+TGG 0.515049 8:+66675880 MsG0880045936.01.T01:CDS
GAAGTGAATGGAACAGTTCG+AGG 0.525952 8:+66675892 MsG0880045936.01.T01:CDS
GATGGAAACTTGGTACCGCC+AGG 0.545312 8:+66675319 MsG0880045936.01.T01:CDS
CTGCCAGTGTGATGCAGACC+TGG 0.547927 8:+66675525 MsG0880045936.01.T01:intron
GAAAGAATTTGAAAAGGATA+GGG 0.554889 8:+66675723 MsG0880045936.01.T01:CDS
AGTGAAGACTGACAGGGAAG+TGG 0.580312 8:+66675837 MsG0880045936.01.T01:CDS
ACAAATGTCACAAGAGCACC+TGG 0.585001 8:-66675337 None:intergenic
GGGACTGCAGTACTTTGAGA+TGG 0.596164 8:+66675366 MsG0880045936.01.T01:CDS
ACGCGGACGAGCAAGAGACA+AGG 0.606388 8:+66675684 MsG0880045936.01.T01:CDS
TTGTGTTACATCACTACCGC+AGG 0.610715 8:+66675238 MsG0880045936.01.T01:CDS
AAGTAAGACTGACAAAGAAG+TGG 0.615033 8:+66675780 MsG0880045936.01.T01:CDS
AAGATCCAAAGGTTGTAACA+AGG 0.661692 8:-66675560 None:intergenic
AGCCGAATGTGTGAAACCTG+CGG 0.704756 8:-66675254 None:intergenic
AGTGGTCAGAGATCAAGACA+AGG 0.725804 8:+66675798 MsG0880045936.01.T01:CDS
AAGGAGCATGAAGCTGAACG+CGG 0.731046 8:+66675667 MsG0880045936.01.T01:CDS
GCATGAAGTGAAGACTGACA+GGG 0.739919 8:+66675831 MsG0880045936.01.T01:CDS
GTACCAAGTTTCCATCAATG+GGG 0.751360 8:-66675312 None:intergenic
AGTGGTCAGAGATCAAGACA+AGG 0.752627 8:+66675855 MsG0880045936.01.T01:CDS
AATGTCACAAGAGCACCTGG+CGG 0.788525 8:-66675334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATGTTGACAAAAATC+AGG - Chr8:66675433-66675452 None:intergenic 20.0%
!!! ATGTAAACTATTTTCACAAA+CGG - Chr8:66675925-66675944 None:intergenic 20.0%
! AAGAAGGAAAAAACTACATA+AGG + Chr8:66675624-66675643 MsG0880045936.01.T01:CDS 25.0%
! AGAAAGAATTTGAAAAGGAT+AGG + Chr8:66675722-66675741 MsG0880045936.01.T01:CDS 25.0%
! ATTAATCAACATGAAACCTA+GGG - Chr8:66675492-66675511 None:intergenic 25.0%
! GAAAGAATTTGAAAAGGATA+GGG + Chr8:66675723-66675742 MsG0880045936.01.T01:CDS 25.0%
! GAGAAGTAAAGAATTAGAAA+AGG + Chr8:66675648-66675667 MsG0880045936.01.T01:CDS 25.0%
! GATGCTAACATTATTCAAAT+TGG - Chr8:66675395-66675414 None:intergenic 25.0%
ACTGTAAAACATGAAGTGAA+TGG + Chr8:66675880-66675899 MsG0880045936.01.T01:CDS 30.0%
ATGTTGACAAAAATCAGGAA+TGG - Chr8:66675428-66675447 None:intergenic 30.0%
CATTAATCAACATGAAACCT+AGG - Chr8:66675493-66675512 None:intergenic 30.0%
CCTAAAAAGATCTACTCAAA+TGG - Chr8:66675460-66675479 None:intergenic 30.0%
CTTGTGACATTTGTTCAAAA+GGG + Chr8:66675346-66675365 MsG0880045936.01.T01:CDS 30.0%
GGCAAATTATTAGTGAAAGA+AGG + Chr8:66675608-66675627 MsG0880045936.01.T01:CDS 30.0%
TAGGGAGAAAGAATTTGAAA+AGG + Chr8:66675717-66675736 MsG0880045936.01.T01:CDS 30.0%
TCTTGTGACATTTGTTCAAA+AGG + Chr8:66675345-66675364 MsG0880045936.01.T01:CDS 30.0%
! CCATTTGAGTAGATCTTTTT+AGG + Chr8:66675457-66675476 MsG0880045936.01.T01:intron 30.0%
! TCTTTTGTGATAAGATCCAA+AGG - Chr8:66675574-66675593 None:intergenic 30.0%
! TTTTTCCTTGTTACAACCTT+TGG + Chr8:66675555-66675574 MsG0880045936.01.T01:CDS 30.0%
AAGATCCAAAGGTTGTAACA+AGG - Chr8:66675563-66675582 None:intergenic 35.0%
AAGTAAGACTGACAAAGAAG+TGG + Chr8:66675780-66675799 MsG0880045936.01.T01:CDS 35.0%
AATCAGGAATGGAAAAACAG+AGG - Chr8:66675417-66675436 None:intergenic 35.0%
ACAAGGAAAAATCTTCATCC+AGG - Chr8:66675546-66675565 None:intergenic 35.0%
AGACAAGGAAAGACATCATA+GGG + Chr8:66675699-66675718 MsG0880045936.01.T01:CDS 35.0%
CAAAAGATGTGCATGAACTT+AGG + Chr8:66675587-66675606 MsG0880045936.01.T01:CDS 35.0%
! ACACATTCGGCTTATACTTT+TGG + Chr8:66675265-66675284 MsG0880045936.01.T01:CDS 35.0%
! TTTTAGGCTTTCATATCCCT+AGG + Chr8:66675473-66675492 MsG0880045936.01.T01:intron 35.0%
ATTAGCAAATGCCCCATTGA+TGG + Chr8:66675301-66675320 MsG0880045936.01.T01:CDS 40.0%
GAGACAAGGAAAGACATCAT+AGG + Chr8:66675698-66675717 MsG0880045936.01.T01:CDS 40.0%
GGTACCAAGTTTCCATCAAT+GGG - Chr8:66675316-66675335 None:intergenic 40.0%
GTACCAAGTTTCCATCAATG+GGG - Chr8:66675315-66675334 None:intergenic 40.0%
AGCATGAAGTGAAGACTGAC+AGG + Chr8:66675830-66675849 MsG0880045936.01.T01:CDS 45.0%
AGTGGTCAGAGATCAAGACA+AGG + Chr8:66675798-66675817 MsG0880045936.01.T01:CDS 45.0%
ATGCCCCATTGATGGAAACT+TGG + Chr8:66675309-66675328 MsG0880045936.01.T01:CDS 45.0%
CGGTACCAAGTTTCCATCAA+TGG - Chr8:66675317-66675336 None:intergenic 45.0%
GAAGTGAATGGAACAGTTCG+AGG + Chr8:66675892-66675911 MsG0880045936.01.T01:CDS 45.0%
GCATGAAGTGAAGACTGACA+GGG + Chr8:66675831-66675850 MsG0880045936.01.T01:CDS 45.0%
TACCGCAGGTTTCACACATT+CGG + Chr8:66675252-66675271 MsG0880045936.01.T01:CDS 45.0%
TTGTGTTACATCACTACCGC+AGG + Chr8:66675238-66675257 MsG0880045936.01.T01:CDS 45.0%
!! ACAAATGTCACAAGAGCACC+TGG - Chr8:66675340-66675359 None:intergenic 45.0%
AGCCGAATGTGTGAAACCTG+CGG - Chr8:66675257-66675276 None:intergenic 50.0%
AGTGAAGACTGACAGGGAAG+TGG + Chr8:66675837-66675856 MsG0880045936.01.T01:CDS 50.0%
! AAGGAGCATGAAGCTGAACG+CGG + Chr8:66675667-66675686 MsG0880045936.01.T01:CDS 50.0%
! GGGACTGCAGTACTTTGAGA+TGG + Chr8:66675366-66675385 MsG0880045936.01.T01:CDS 50.0%
!! AATGTCACAAGAGCACCTGG+CGG - Chr8:66675337-66675356 None:intergenic 50.0%
CATCCAGGTCTGCATCACAC+TGG - Chr8:66675531-66675550 None:intergenic 55.0%
GATGGAAACTTGGTACCGCC+AGG + Chr8:66675319-66675338 MsG0880045936.01.T01:CDS 55.0%
ACGCGGACGAGCAAGAGACA+AGG + Chr8:66675684-66675703 MsG0880045936.01.T01:CDS 60.0%
CAGGTCTGCATCACACTGGC+AGG - Chr8:66675527-66675546 None:intergenic 60.0%
CTGCCAGTGTGATGCAGACC+TGG + Chr8:66675525-66675544 MsG0880045936.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 66675238 66676014 66675238 ID=MsG0880045936.01;Name=MsG0880045936.01
Chr8 mRNA 66675238 66676014 66675238 ID=MsG0880045936.01.T01;Parent=MsG0880045936.01;Name=MsG0880045936.01.T01;_AED=0.48;_eAED=0.56;_QI=0|1|0|1|1|1|2|0|216
Chr8 exon 66675238 66675405 66675238 ID=MsG0880045936.01.T01:exon:25298;Parent=MsG0880045936.01.T01
Chr8 exon 66675532 66676014 66675532 ID=MsG0880045936.01.T01:exon:25299;Parent=MsG0880045936.01.T01
Chr8 CDS 66675238 66675405 66675238 ID=MsG0880045936.01.T01:cds;Parent=MsG0880045936.01.T01
Chr8 CDS 66675532 66676014 66675532 ID=MsG0880045936.01.T01:cds;Parent=MsG0880045936.01.T01
Gene Sequence

>MsG0880045936.01.T01

TTGTGTTACATCACTACCGCAGGTTTCACACATTCGGCTTATACTTTTGGTAATGAAGCTTCTATTAGCAAATGCCCCATTGATGGAAACTTGGTACCGCCAGGTGCTCTTGTGACATTTGTTCAAAAGGGACTGCAGTACTTTGAGATGGAAGCCAATTTGAATAATTGTGATGCAGACCTGGATGAAGATTTTTCCTTGTTACAACCTTTGGATCTTATCACAAAAGATGTGCATGAACTTAGGCAAATTATTAGTGAAAGAAGGAAAAAACTACATAAGGAGAGAAGTAAAGAATTAGAAAAGGAGCATGAAGCTGAACGCGGACGAGCAAGAGACAAGGAAAGACATCATAGGGAGAAAGAATTTGAAAAGGATAGGGTAAAAATTGCTAAAGAGCGTGAGCAGCAGCATGTAAGTAAGACTGACAAAGAAGTGGTCAGAGATCAAGACAAGGTTACTGCAAAGCATGAAGTGAAGACTGACAGGGAAGTGGTCAGAGATCAAGACAAGGTCACTGTAAAACATGAAGTGAATGGAACAGTTCGAGGTAATATGCCGTTTGTGAAAATAGTTTACATTACGTATATCACATATGTTCTTTGTATTGTGTTTTGTGTTCAGCTGCATTTTCTAATCAGTAATCTGTGA

Protein sequence

>MsG0880045936.01.T01

LCYITTAGFTHSAYTFGNEASISKCPIDGNLVPPGALVTFVQKGLQYFEMEANLNNCDADLDEDFSLLQPLDLITKDVHELRQIISERRKKLHKERSKELEKEHEAERGRARDKERHHREKEFEKDRVKIAKEREQQHVSKTDKEVVRDQDKVTAKHEVKTDREVVRDQDKVTVKHEVNGTVRGNMPFVKIVYITYITYVLCIVFCVQLHFLISNL*