Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020538.01.T01 | XP_003606113.1 | 98.571 | 420 | 6 | 0 | 21 | 440 | 1 | 420 | 0 | 848 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015245.01 | MsG0480020538.01 | 0.807645 | 4.568328e-50 | 2.558141e-47 |
| MsG0480020538.01 | MsG0880042368.01 | 0.814362 | 1.601522e-51 | 1.072372e-48 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020538.01.T01 | MTR_4g053260 | 98.571 | 420 | 6 | 0 | 21 | 440 | 1 | 420 | 0.0 | 848 |
| MsG0480020538.01.T01 | MTR_1g060040 | 66.826 | 419 | 139 | 0 | 22 | 440 | 1 | 419 | 0.0 | 546 |
| MsG0480020538.01.T01 | MTR_7g104490 | 63.484 | 419 | 153 | 0 | 22 | 440 | 1 | 419 | 0.0 | 526 |
Find 92 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATGGATGTGGTGAAAT+TGG | 0.318949 | 4:-43028145 | None:intergenic |
| AACGAAAGTGATTTCAAACA+CGG | 0.631215 | 4:-43027403 | None:intergenic |
| AACTCAGAGATAAATGACTC+AGG | 0.621606 | 4:-43027912 | None:intergenic |
| AAGCAGCCCCTCGACCCACT+GGG | 0.569654 | 4:-43028040 | None:intergenic |
| AAGGGATGTGGTGATAGCCT+CGG | 0.602611 | 4:+43027015 | MsG0480020538.01.T01:CDS |
| AAGTTTATATTGGAGGCTGA+TGG | 0.502708 | 4:+43027598 | MsG0480020538.01.T01:CDS |
| AATGGAAAGCGACTGCAACC+CGG | 0.628343 | 4:-43027176 | None:intergenic |
| ACATCTTGACATTGGTGAGA+CGG | 0.597711 | 4:+43027720 | MsG0480020538.01.T01:CDS |
| ACATTCTGGAGATTTGTGGG+AGG | 0.676084 | 4:+43027311 | MsG0480020538.01.T01:CDS |
| ACTCAGGTATGGAAAGCAAG+TGG | 0.667455 | 4:-43027896 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACGAAAGTGATTTCAAACA+CGG | - | Chr4:43027406-43027425 | None:intergenic | 30.0% | |
| AGTGTTATACATCTTGACAT+TGG | + | Chr4:43027712-43027731 | MsG0480020538.01.T01:CDS | 30.0% | |
| ATCTCCAGAATGTTAACATT+CGG | - | Chr4:43027304-43027323 | None:intergenic | 30.0% | |
| CAGTTTGGACAAGTTTATAT+TGG | + | Chr4:43027588-43027607 | MsG0480020538.01.T01:CDS | 30.0% | |
| CGAGATCTTCAAATTTAAAG+AGG | + | Chr4:43027812-43027831 | MsG0480020538.01.T01:CDS | 30.0% | |
| CTTCAAATTTAAAGAGGCTT+AGG | + | Chr4:43027818-43027837 | MsG0480020538.01.T01:CDS | 30.0% | |
| GATTTGCAAGTTTAACATGA+TGG | + | Chr4:43026940-43026959 | MsG0480020538.01.T01:CDS | 30.0% | |
| TCATGTTAAACTTGCAAATC+CGG | - | Chr4:43026940-43026959 | None:intergenic | 30.0% | |
| TTATGTTGAAGCAATCAGTT+TGG | + | Chr4:43027573-43027592 | MsG0480020538.01.T01:CDS | 30.0% | |
| TTTGGACAAGTTTATATTGG+AGG | + | Chr4:43027591-43027610 | MsG0480020538.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 43026896 | 43028218 | 43026896 | ID=MsG0480020538.01;Name=MsG0480020538.01 |
| Chr4 | mRNA | 43026896 | 43028218 | 43026896 | ID=MsG0480020538.01.T01;Parent=MsG0480020538.01;Name=MsG0480020538.01.T01;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|440 |
| Chr4 | exon | 43026896 | 43028218 | 43026896 | ID=MsG0480020538.01.T01:exon:5142;Parent=MsG0480020538.01.T01 |
| Chr4 | CDS | 43026896 | 43028218 | 43026896 | ID=MsG0480020538.01.T01:cds;Parent=MsG0480020538.01.T01 |
| Gene Sequence |
>MsG0480020538.01.T01 ATGTCTAACACAGGAAACTATGTTTGTATTGTGAAGTTGTGCCGGATTTGCAAGTTTAACATGATGGATCAGCTTCCTGTTGAAGTGATTGGGAACATACTATCCCATCTCAAGGCTGCAAGGGATGTGGTGATAGCCTCGGCAACATGCAAGAAATGGCGAATTGCATGCTGCAAACATCTCCACACATTGTCCTTTAGCTCGAATGATTGGTCGGTTTACCGCGATTTGTCAACGACCCGTCTTGAGATTTTGATCACACAGACTATTTTTCAGACATCCGGGTTGCAGTCGCTTTCCATTTTGATGGAAGATGTCGATGAATTCTCGGCGTCTGCTGTTATTGCTTGGCTTATGTACACCAGGGAGACTCTGAGGCAATTGTTTTATAATGTGAAGACTATGCCGAATGTTAACATTCTGGAGATTTGTGGGAGGCATAAGCTGGAAGTACTTGATTTGGAGCATAATTCCATTGTTGGGGTTGAGCCGAATTATCAGAGGTTTCCGTGTTTGAAATCACTTTCGTTGAGTTGTGTTAGTATATCTGCGTTGGATTTGAGTCTTTTGGTGTCTGCGTGTCCGAGGATTGAAGCTTTGGAGCTTGTAAATCCGGAGATTGCAATGTCTGATGCAATGGTGACCGTTGAATTGAGTAGCTCGACGCTCAAGAGTGTTTATGTTGAAGCAATCAGTTTGGACAAGTTTATATTGGAGGCTGATGGGATTGAGTCTCTTCACTTGAAAGATTGTGCGCTTGAAGTTTTTGAACTCATTGGCAAGGGGACCTTGAAGAATTTTAGGATCGACGATGTTAGTGTTATACATCTTGACATTGGTGAGACGGTCGAGAATCTTGAGAGTGTGGATATCAGCAACTTCACAATCATTTGGCCAAAATTTTACCAGATGATTTCGAGATCTTCAAATTTAAAGAGGCTTAGGCTTTGGGATGTGATGTTCGATGATGAGGATGAGGTTGTGGATTTGGAAACAATCGCCACTTGCTTTCCATACCTGAGTCATTTATCTCTGAGTTATGATGTGAGAGATGGAGTGCTTCACTATGGTTTACAAGGCTCTTCTCATCTGGAGAATGTTGTTGTTTTGGAGCTTGGCTGGAATGTGATTAATGATCTTTTCTCCCAGTGGGTCGAGGGGCTGCTTAAGCGATGCCCTATTCTCAAGAAACTAGTCATTCACGGTGTTGTTTCCGAGGCTAAGTCTGATGAAGAATGCAAGATGTTAGCCAATTTCACCACATCCATGGTTGAGCTGATGAGGAGATACACAGATGTAGATCCACATTTTAAGTTTGAATAA |
| Protein sequence |
>MsG0480020538.01.T01 MSNTGNYVCIVKLCRICKFNMMDQLPVEVIGNILSHLKAARDVVIASATCKKWRIACCKHLHTLSFSSNDWSVYRDLSTTRLEILITQTIFQTSGLQSLSILMEDVDEFSASAVIAWLMYTRETLRQLFYNVKTMPNVNILEICGRHKLEVLDLEHNSIVGVEPNYQRFPCLKSLSLSCVSISALDLSLLVSACPRIEALELVNPEIAMSDAMVTVELSSSTLKSVYVEAISLDKFILEADGIESLHLKDCALEVFELIGKGTLKNFRIDDVSVIHLDIGETVENLESVDISNFTIIWPKFYQMISRSSNLKRLRLWDVMFDDEDEVVDLETIATCFPYLSHLSLSYDVRDGVLHYGLQGSSHLENVVVLELGWNVINDLFSQWVEGLLKRCPILKKLVIHGVVSEAKSDEECKMLANFTTSMVELMRRYTDVDPHFKFE* |
