AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011360.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011360.01.T01 MTR_3g082890 44.602 352 157 9 53 374 141 484 5.72e-77 245
MsG0280011360.01.T01 MTR_4g134150 70.000 170 49 2 65 233 62 230 5.20e-73 227
MsG0280011360.01.T01 MTR_3g082890 40.360 389 157 10 53 374 61 441 4.22e-70 226
MsG0280011360.01.T01 MTR_7g098510 40.798 326 167 12 68 374 73 391 6.00e-65 211
MsG0280011360.01.T01 MTR_1g041245 42.900 331 157 14 67 374 65 386 1.03e-63 208
MsG0280011360.01.T01 MTR_7g033915 41.104 326 147 12 67 373 65 364 2.06e-61 202
MsG0280011360.01.T01 MTR_3g115430 72.000 125 35 0 250 374 1 125 7.49e-61 192
MsG0280011360.01.T01 MTR_7g032380 40.752 319 151 11 67 374 65 356 8.49e-60 197
MsG0280011360.01.T01 MTR_7g034115 40.606 330 156 12 67 372 65 378 3.05e-58 194
MsG0280011360.01.T01 MTR_7g032370 41.284 327 155 14 67 374 44 352 7.02e-58 192
MsG0280011360.01.T01 MTR_7g098570 40.625 320 161 13 68 368 75 384 8.15e-57 193
MsG0280011360.01.T01 MTR_7g033990 40.432 324 157 9 67 368 65 374 1.18e-56 191
MsG0280011360.01.T01 MTR_7g034120 39.130 322 159 11 76 374 1 308 3.04e-56 186
MsG0280011360.01.T01 MTR_7g056433 38.623 334 169 12 67 374 66 389 1.99e-53 181
MsG0280011360.01.T01 MTR_7g115270 37.278 338 176 9 62 368 61 393 2.30e-52 179
MsG0280011360.01.T01 MTR_0543s0010 37.975 316 179 9 68 373 141 449 3.95e-52 179
MsG0280011360.01.T01 MTR_7g098480 40.841 333 167 13 50 368 64 380 5.46e-52 180
MsG0280011360.01.T01 MTR_7g081290 64.964 137 42 4 241 374 123 256 1.96e-51 172
MsG0280011360.01.T01 MTR_2g020180 52.353 170 72 2 205 374 55 215 1.24e-50 169
MsG0280011360.01.T01 MTR_5g012840 37.695 321 144 13 66 374 42 318 4.51e-49 168
MsG0280011360.01.T01 MTR_7g088260 36.111 324 185 10 67 374 145 462 3.50e-48 169
MsG0280011360.01.T01 MTR_0492s0020 37.748 302 174 8 68 362 141 435 9.88e-48 167
MsG0280011360.01.T01 MTR_7g056420 36.778 329 156 12 62 368 58 356 1.00e-44 165
MsG0280011360.01.T01 MTR_7g056430 36.778 329 156 12 62 368 58 356 1.27e-44 165
MsG0280011360.01.T01 MTR_3g036250 35.556 315 176 10 66 368 58 357 2.48e-43 154
MsG0280011360.01.T01 MTR_3g108560 35.714 322 185 9 67 373 74 388 1.84e-42 152
MsG0280011360.01.T01 MTR_5g013500 34.584 373 188 15 36 374 15 365 1.50e-41 149
MsG0280011360.01.T01 MTR_5g008920 33.754 317 183 10 76 374 74 381 3.05e-41 149
MsG0280011360.01.T01 MTR_8g085430 35.331 317 132 11 62 365 60 316 4.42e-40 145
MsG0280011360.01.T01 MTR_3g023630 35.032 314 182 10 67 368 62 365 6.88e-39 142
MsG0280011360.01.T01 MTR_3g108150 34.731 334 185 11 63 373 63 386 9.94e-39 142
MsG0280011360.01.T01 MTR_3g087260 39.493 276 151 8 111 373 184 456 1.01e-38 144
MsG0280011360.01.T01 MTR_5g008950 31.494 308 186 11 85 374 65 365 1.01e-38 142
MsG0280011360.01.T01 MTR_3g040700 34.965 286 164 9 66 340 96 370 6.22e-38 141
MsG0280011360.01.T01 MTR_3g467400 33.431 341 164 12 67 374 57 367 1.26e-36 136
MsG0280011360.01.T01 MTR_7g114710 33.535 331 164 12 67 374 67 364 8.40e-36 134
MsG0280011360.01.T01 MTR_1g066830 31.447 318 174 12 85 373 84 386 2.06e-34 131
MsG0280011360.01.T01 MTR_3g107120 32.229 332 183 13 67 374 78 391 2.45e-34 131
MsG0280011360.01.T01 MTR_1g043170 62.500 88 32 1 288 374 1 88 2.79e-34 122
MsG0280011360.01.T01 MTR_5g007410 33.634 333 174 13 66 367 82 398 4.33e-34 131
MsG0280011360.01.T01 MTR_7g034110 33.333 312 153 11 67 367 65 332 2.49e-32 124
MsG0280011360.01.T01 MTR_3g107100 34.114 299 164 11 68 344 69 356 2.86e-32 125
MsG0280011360.01.T01 MTR_4g080610 33.022 321 171 13 67 373 66 356 8.92e-32 123
MsG0280011360.01.T01 MTR_6g045333 29.859 355 197 12 45 373 117 445 4.02e-31 123
MsG0280011360.01.T01 MTR_2g007450 27.493 371 194 13 62 374 68 421 4.07e-31 122
MsG0280011360.01.T01 MTR_1g108820 32.302 291 156 8 104 367 100 376 1.55e-30 120
MsG0280011360.01.T01 MTR_3g105060 32.119 302 172 10 87 374 91 373 4.14e-30 119
MsG0280011360.01.T01 MTR_5g058100 30.449 312 185 9 84 374 84 384 1.38e-29 117
MsG0280011360.01.T01 MTR_7g007080 30.091 329 182 11 68 368 62 370 1.83e-29 117
MsG0280011360.01.T01 MTR_7g056020 34.505 313 178 12 76 374 72 371 4.79e-29 119
MsG0280011360.01.T01 MTR_5g073420 30.000 310 181 11 52 344 54 344 1.50e-28 115
MsG0280011360.01.T01 MTR_6g022500 30.719 306 173 9 84 366 176 465 3.63e-28 115
MsG0280011360.01.T01 MTR_3g113785 58.889 90 29 1 253 334 3 92 7.32e-28 105
MsG0280011360.01.T01 MTR_3g109982 37.402 254 139 10 127 367 220 466 9.41e-28 115
MsG0280011360.01.T01 MTR_3g109982 41.085 129 73 3 65 191 65 192 1.92e-15 78.6
MsG0280011360.01.T01 MTR_7g026500 30.696 316 183 10 84 373 89 394 1.01e-27 113
MsG0280011360.01.T01 MTR_3g107160 29.167 336 190 15 68 374 67 383 4.70e-27 110
MsG0280011360.01.T01 MTR_3g071850 26.786 392 248 12 2 367 21 399 7.61e-27 112
MsG0280011360.01.T01 MTR_5g058000 31.190 311 174 9 84 373 83 374 1.24e-26 109
MsG0280011360.01.T01 MTR_5g045170 29.878 328 201 12 62 367 61 381 1.30e-26 109
MsG0280011360.01.T01 MTR_4g039440 28.358 335 209 9 65 374 84 412 2.89e-26 109
MsG0280011360.01.T01 MTR_1g108800 33.803 284 152 11 102 367 67 332 1.48e-25 105
MsG0280011360.01.T01 MTR_5g063950 30.796 289 175 10 102 373 81 361 1.90e-25 105
MsG0280011360.01.T01 MTR_6g040270 28.526 312 181 10 85 371 84 378 6.73e-25 104
MsG0280011360.01.T01 MTR_1g053025 28.235 340 196 13 68 372 61 387 1.74e-24 103
MsG0280011360.01.T01 MTR_2g036470 29.321 324 200 9 66 367 70 386 2.72e-24 103
MsG0280011360.01.T01 MTR_1089s0010 30.195 308 185 12 85 374 84 379 7.50e-24 102
MsG0280011360.01.T01 MTR_1g085120 28.344 314 193 8 84 374 167 471 2.44e-23 101
MsG0280011360.01.T01 MTR_1g085120 28.344 314 193 8 84 374 242 546 4.22e-23 101
MsG0280011360.01.T01 MTR_5g031970 30.293 307 191 10 66 366 56 345 4.37e-23 99.4
MsG0280011360.01.T01 MTR_7g083740 28.763 299 165 10 99 373 92 366 5.95e-23 99.4
MsG0280011360.01.T01 MTR_0009s0080 32.090 268 138 9 104 344 117 367 8.43e-23 99.4
MsG0280011360.01.T01 MTR_6g011460 30.634 284 171 9 79 347 116 388 1.07e-22 99.0
MsG0280011360.01.T01 MTR_6g044572 28.819 288 179 9 95 374 119 388 1.71e-22 98.2
MsG0280011360.01.T01 MTR_2g007400 27.635 351 187 16 68 371 70 400 3.71e-22 97.4
MsG0280011360.01.T01 MTR_6g044560 29.514 288 177 9 95 374 295 564 4.46e-22 98.2
MsG0280011360.01.T01 MTR_7g056277 29.697 330 194 11 65 373 315 627 6.10e-22 97.8
MsG0280011360.01.T01 MTR_3g108560 33.766 231 135 7 67 286 74 297 9.74e-22 94.7
MsG0280011360.01.T01 MTR_5g039490 29.779 272 161 7 102 367 119 366 1.67e-21 95.1
MsG0280011360.01.T01 MTR_5g039450 28.144 334 174 13 66 372 62 356 1.76e-21 94.7
MsG0280011360.01.T01 MTR_7g056287 29.429 333 191 13 65 373 59 371 2.73e-21 94.4
MsG0280011360.01.T01 MTR_5g039470 27.517 298 182 10 66 340 41 327 7.41e-21 92.4
MsG0280011360.01.T01 MTR_5g073370 30.241 291 178 10 66 344 70 347 7.50e-21 93.2
MsG0280011360.01.T01 MTR_7g055943 28.012 332 206 11 68 374 98 421 8.65e-21 93.6
MsG0280011360.01.T01 MTR_2g007550 30.675 326 145 17 68 374 72 335 1.58e-20 92.0
MsG0280011360.01.T01 MTR_6g022520 28.840 319 199 9 76 373 1 312 2.80e-20 90.9
MsG0280011360.01.T01 MTR_5g054920 30.108 186 105 6 174 344 2 177 6.63e-20 87.0
MsG0280011360.01.T01 MTR_3g113770 33.023 215 73 7 131 334 180 334 6.69e-20 90.1
MsG0280011360.01.T01 MTR_1g107385 28.409 352 183 15 68 371 90 420 1.44e-19 90.1
MsG0280011360.01.T01 MTR_5g045510 27.692 325 186 11 62 365 64 360 1.75e-19 89.4
MsG0280011360.01.T01 MTR_5g065100 27.507 349 209 13 31 373 35 345 3.36e-19 89.0
MsG0280011360.01.T01 MTR_2g102690 53.409 88 38 2 74 158 12 99 3.80e-19 83.2
MsG0280011360.01.T01 MTR_5g061680 31.102 254 139 9 67 312 69 294 6.93e-19 87.0
MsG0280011360.01.T01 MTR_6g022360 28.955 335 196 12 67 373 87 407 1.18e-18 87.0
MsG0280011360.01.T01 MTR_3g026920 26.284 331 168 10 68 346 77 383 4.16e-18 85.5
MsG0280011360.01.T01 MTR_7g007340 29.572 257 150 9 127 368 108 348 5.16e-17 82.0
MsG0280011360.01.T01 MTR_5g083890 27.372 274 155 9 127 373 142 398 5.82e-17 82.8
MsG0280011360.01.T01 MTR_3g107940 42.857 105 50 2 265 368 109 204 5.82e-17 81.6
MsG0280011360.01.T01 MTR_7g034090 27.129 317 148 14 68 373 67 311 1.52e-16 80.9
MsG0280011360.01.T01 MTR_7g056447 40.650 123 70 2 67 186 63 185 1.61e-16 79.7
MsG0280011360.01.T01 MTR_3g106960 35.294 136 75 4 244 372 64 193 2.00e-16 78.2
MsG0280011360.01.T01 MTR_2g005990 28.443 334 185 13 70 371 81 392 2.29e-16 80.1
MsG0280011360.01.T01 MTR_3g099830 28.107 338 177 15 66 371 68 371 3.14e-16 79.7
MsG0280011360.01.T01 MTR_5g008910 30.901 233 117 9 177 374 79 302 1.59e-15 77.0
MsG0280011360.01.T01 MTR_3g110010 41.085 129 73 3 65 191 65 192 2.51e-15 78.2
MsG0280011360.01.T01 MTR_3g108140 43.210 81 43 1 296 373 67 147 3.04e-15 73.6
MsG0280011360.01.T01 MTR_2g007360 26.296 270 152 10 136 371 115 371 3.83e-15 76.6
MsG0280011360.01.T01 MTR_1377s0010 35.577 104 61 2 241 344 30 127 6.80e-15 71.6
MsG0280011360.01.T01 MTR_5g076150 31.308 214 108 7 127 309 22 227 8.28e-15 73.6
MsG0280011360.01.T01 MTR_7g075820 26.038 265 161 10 127 371 142 391 9.34e-15 75.5
MsG0280011360.01.T01 MTR_5g061410 31.122 196 118 6 102 286 78 267 9.55e-15 73.9
MsG0280011360.01.T01 MTR_8g077460 26.389 288 178 11 100 374 109 375 9.87e-15 75.1
MsG0280011360.01.T01 MTR_5g073340 26.936 297 176 9 51 321 27 308 2.83e-14 73.2
MsG0280011360.01.T01 MTR_4g096730 46.341 82 36 2 297 374 12 89 3.26e-14 68.6
MsG0280011360.01.T01 MTR_7g033945 41.284 109 53 4 67 165 65 172 3.31e-14 70.9
MsG0280011360.01.T01 MTR_2g007090 27.311 238 126 6 153 373 86 293 5.85e-14 72.0
MsG0280011360.01.T01 MTR_2g008310 27.308 260 162 8 131 374 123 371 7.52e-14 72.8
MsG0280011360.01.T01 MTR_5g024060 31.538 130 82 2 238 367 76 198 1.52e-13 70.5
MsG0280011360.01.T01 MTR_1g108820 30.622 209 109 6 104 286 100 298 1.88e-13 71.2
MsG0280011360.01.T01 MTR_3g095630 34.375 128 76 4 241 367 55 175 4.67e-13 67.8
MsG0280011360.01.T01 MTR_1g043180 48.214 56 29 0 236 291 42 97 1.83e-12 65.5
MsG0280011360.01.T01 MTR_8g014210 26.941 219 115 6 193 374 21 231 1.99e-12 66.6
MsG0280011360.01.T01 MTR_3g094980 36.667 90 52 1 266 350 34 123 3.52e-12 64.7
MsG0280011360.01.T01 MTR_7g056280 26.738 187 110 5 178 344 367 546 1.53e-11 66.2
MsG0280011360.01.T01 MTR_5g073400 39.450 109 64 2 85 192 7 114 1.57e-11 62.0
MsG0280011360.01.T01 MTR_2g007500 26.721 247 157 7 100 324 91 335 2.06e-11 65.1
MsG0280011360.01.T01 MTR_8g014160 27.576 330 217 13 62 374 101 425 6.48e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011360.01.T01 AT5G56370 27.202 386 206 10 58 373 40 420 5.03e-21 94.4
MsG0280011360.01.T01 AT5G56370 27.202 386 206 10 58 373 40 420 5.03e-21 94.4
MsG0280011360.01.T01 AT4G26340 26.087 368 207 13 67 374 57 419 1.53e-19 90.1
MsG0280011360.01.T01 AT5G62970 25.313 399 221 14 49 373 53 448 6.66e-18 85.1
MsG0280011360.01.T01 AT5G38590 25.466 322 172 8 99 362 90 401 1.09e-14 75.5
MsG0280011360.01.T01 AT4G10400 27.988 343 184 14 85 373 75 408 2.84e-14 73.9
MsG0280011360.01.T01 AT4G10400 27.988 343 184 14 85 373 75 408 2.84e-14 73.9
MsG0280011360.01.T01 AT4G10400 27.988 343 184 14 85 373 75 408 2.84e-14 73.9
MsG0280011360.01.T01 AT1G32375 23.155 393 216 16 58 374 40 422 8.06e-14 72.8
MsG0280011360.01.T01 AT1G32375 23.155 393 216 16 58 374 40 422 8.06e-14 72.8
MsG0280011360.01.T01 AT5G38590 35.000 140 85 4 99 232 90 229 8.67e-13 68.6
MsG0280011360.01.T01 AT5G38590 35.252 139 84 4 100 232 56 194 1.40e-12 67.4
MsG0280011360.01.T01 AT3G49030 24.599 374 210 13 65 373 74 440 2.12e-12 68.6
MsG0280011360.01.T01 AT3G26920 24.516 310 198 11 90 373 1 300 4.83e-12 66.6
MsG0280011360.01.T01 AT5G52460 23.636 275 179 8 93 348 65 327 6.13e-12 66.6
MsG0280011360.01.T01 AT5G56370 35.000 140 79 4 58 186 40 178 1.00e-11 65.9
MsG0280011360.01.T01 AT4G10420 25.987 304 168 10 122 373 23 321 2.07e-11 64.7
MsG0280011360.01.T01 AT3G54910 27.143 350 184 13 85 374 35 373 4.68e-11 64.3
MsG0280011360.01.T01 AT3G54910 27.143 350 184 13 85 374 35 373 4.68e-11 64.3
MsG0280011360.01.T01 AT3G54910 27.143 350 184 13 85 374 35 373 4.68e-11 64.3

Find 80 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTTTGCATTTAAATTTA+AGG 0.083917 2:-82968829 None:intergenic
TTGAATTAAACTGTCAAAAT+TGG 0.098936 2:-82968413 None:intergenic
TCATGAATATATTGACTTTC+AGG 0.143726 2:-82968244 None:intergenic
CGATCATTTCGCCTCAAATT+TGG 0.183656 2:+82967863 MsG0280011360.01.T01:CDS
ATCGCCAAATTGGGCGTTTC+TGG 0.184147 2:-82968211 None:intergenic
CTAAGAACTTGCTTGTTTAC+AGG 0.209416 2:+82968714 MsG0280011360.01.T01:CDS
AATACTTGTTATCGCCAAAT+TGG 0.225019 2:-82968221 None:intergenic
GACATGTTCGAGAAGTTTAA+TGG 0.229648 2:+82967962 MsG0280011360.01.T01:CDS
ATGCATTCCGGAGCAATATC+TGG 0.246429 2:-82968681 None:intergenic
TGTTTACAGGACATCGAAAA+TGG 0.276176 2:+82968633 MsG0280011360.01.T01:intron
AAGAGAATCTTGTCAATATC+TGG 0.290913 2:-82968124 None:intergenic
TACTCCAGAAACGCCCAATT+TGG 0.301204 2:+82968207 MsG0280011360.01.T01:CDS
TGCATTTAAATTTAAGGAAT+AGG 0.319894 2:-82968823 None:intergenic
TGAATTAAACTGTCAAAATT+GGG 0.326843 2:-82968412 None:intergenic
GATATTGACAAGATTCTCTT+AGG 0.328674 2:+82968127 MsG0280011360.01.T01:CDS
CGAAACGGTGGAATCCATTA+TGG 0.335044 2:+82967734 MsG0280011360.01.T01:CDS
ATGGAATTTATGAGGACAAT+TGG 0.341820 2:+82968652 MsG0280011360.01.T01:CDS
TGATACAATGACCGAGTATA+TGG 0.354546 2:-82967819 None:intergenic
TATACTCAAAGTTCTCTGTT+TGG 0.360098 2:+82968078 MsG0280011360.01.T01:CDS
TTAATTTAAAAGGTAAAAGT+TGG 0.360917 2:+82968452 MsG0280011360.01.T01:CDS
GGTGATGTGTCTTTGAAGTT+TGG 0.365195 2:-82967786 None:intergenic
GATCCATTCCAACAGAAGAT+AGG 0.384831 2:+82967629 MsG0280011360.01.T01:CDS
AGAGAATATGCGTTTAAAAC+TGG 0.387449 2:+82968285 MsG0280011360.01.T01:CDS
TGTTTGCAATTCCTCTAGAA+TGG 0.393315 2:-82968155 None:intergenic
ATGTGAGATATAATTGAATC+TGG 0.402764 2:-82967664 None:intergenic
GTTTGCAATTCCTCTAGAAT+GGG 0.408530 2:-82968154 None:intergenic
AATTGTCGTCTTTCACAGAT+AGG 0.413879 2:-82968039 None:intergenic
GTGATGTGTCTTTGAAGTTT+GGG 0.419676 2:-82967785 None:intergenic
GCTAGTGGCGACGGAGTGTT+TGG 0.424993 2:-82967704 None:intergenic
GTATAGCTGCATTAATAAAA+CGG 0.430678 2:-82967913 None:intergenic
AGCTGAGATGAAATGCATTC+CGG 0.438266 2:-82968693 None:intergenic
ATACTTGTTATCGCCAAATT+GGG 0.441599 2:-82968220 None:intergenic
TCGAGAACCGAGAGCCATAA+TGG 0.441651 2:-82967748 None:intergenic
TAATTTAAAAGGTAAAAGTT+GGG 0.447111 2:+82968453 MsG0280011360.01.T01:CDS
AAACAGAGAACTTTGAGTAT+AGG 0.461425 2:-82968076 None:intergenic
CATATTCTATGCCAAATTTG+AGG 0.478293 2:-82967874 None:intergenic
TTGAGGCGAAATGATCGTAT+TGG 0.479233 2:-82967857 None:intergenic
AACCCTATCTTCTGTTGGAA+TGG 0.483576 2:-82967632 None:intergenic
GAGCTAACCCTATCTTCTGT+TGG 0.488748 2:-82967637 None:intergenic
CATCGAAAATGGAATTTATG+AGG 0.500230 2:+82968644 MsG0280011360.01.T01:CDS
GGAAATTATCTGTGTGCCCA+AGG 0.500405 2:+82968862 MsG0280011360.01.T01:CDS
TTGGATAATATGTTGTTCAA+TGG 0.500733 2:+82968097 MsG0280011360.01.T01:CDS
ATGCAAAGCACGAAATGTTA+CGG 0.503024 2:+82968841 MsG0280011360.01.T01:CDS
ATGGTAAGATGTTGAAGCTT+GGG 0.515978 2:-82968517 None:intergenic
ATTAATGCAGCTATACAAAG+AGG 0.517042 2:+82967920 MsG0280011360.01.T01:CDS
CATATGTAATACCTCATGAA+TGG 0.521023 2:-82968536 None:intergenic
ATCCATTCCAACAGAAGATA+GGG 0.524022 2:+82967630 MsG0280011360.01.T01:CDS
GTAAAAGTTGGGATAAGAAG+TGG 0.524392 2:+82968464 MsG0280011360.01.T01:CDS
AATATAAGTTCACAGCCCCT+TGG 0.525982 2:-82968879 None:intergenic
AGCTGCATTAATAAAACGGT+TGG 0.531777 2:-82967909 None:intergenic
GTGGCGACGGAGTGTTTGGT+TGG 0.533987 2:-82967700 None:intergenic
GTTGGGATAAGAAGTGGAAG+TGG 0.534670 2:+82968470 MsG0280011360.01.T01:CDS
CCACTAGCGTCCTCTCGAAA+CGG 0.539101 2:+82967719 MsG0280011360.01.T01:CDS
TGCATTCCGGAGCAATATCT+GGG 0.540986 2:-82968680 None:intergenic
ATATAAGTTCACAGCCCCTT+GGG 0.543168 2:-82968878 None:intergenic
GAAATTATCTGTGTGCCCAA+GGG 0.545744 2:+82968863 MsG0280011360.01.T01:CDS
TGCTTGTTTACAGGCTGCAG+AGG 0.549690 2:+82968723 MsG0280011360.01.T01:CDS
TTCATGAATACTTCCTACCT+AGG 0.553778 2:+82968260 MsG0280011360.01.T01:CDS
CGAGTATATGGCATGGCGAA+AGG 0.557661 2:-82967807 None:intergenic
CTTTCGCCATGCCATATACT+CGG 0.567046 2:+82967808 MsG0280011360.01.T01:CDS
CTTAGGCTGCCCCATTCTAG+AGG 0.573194 2:+82968144 MsG0280011360.01.T01:CDS
AATGGTAAGATGTTGAAGCT+TGG 0.575823 2:-82968518 None:intergenic
GTGGAATCCATTATGGCTCT+CGG 0.575983 2:+82967741 MsG0280011360.01.T01:CDS
GAATTAAACTGTCAAAATTG+GGG 0.577717 2:-82968411 None:intergenic
ATGGATTCCACCGTTTCGAG+AGG 0.580028 2:-82967729 None:intergenic
AATGGATCGATGAGGTTGAG+AGG 0.584713 2:-82967614 None:intergenic
GAAGGACCCAGATATTGCTC+CGG 0.586927 2:+82968674 MsG0280011360.01.T01:CDS
AAACGCATATTCTCTACCCT+AGG 0.589888 2:-82968277 None:intergenic
AATTTATGAGGACAATTGGA+AGG 0.595790 2:+82968656 MsG0280011360.01.T01:CDS
CGAGAGGACGCTAGTGGCGA+CGG 0.595932 2:-82967713 None:intergenic
ATAGGCACTGTGAATTGTCA+TGG 0.604532 2:-82968805 None:intergenic
GCATATTCTCTACCCTAGGT+AGG 0.607522 2:-82968273 None:intergenic
TCATGAATACTTCCTACCTA+GGG 0.617474 2:+82968261 MsG0280011360.01.T01:CDS
AAATTATCTGTGTGCCCAAG+GGG 0.625212 2:+82968864 MsG0280011360.01.T01:CDS
CTAGCGTCCTCTCGAAACGG+TGG 0.629979 2:+82967722 MsG0280011360.01.T01:CDS
TCAACATCTTACCATTCATG+AGG 0.633550 2:+82968525 MsG0280011360.01.T01:CDS
CCGTTTCGAGAGGACGCTAG+TGG 0.642179 2:-82967719 None:intergenic
TTTGCAATTCCTCTAGAATG+GGG 0.642459 2:-82968153 None:intergenic
CAATGACCGAGTATATGGCA+TGG 0.667166 2:-82967814 None:intergenic
TCTGTTGGAATGGATCGATG+AGG 0.739815 2:-82967622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTATTTTTCTTTAATTTAAA+AGG + Chr2:82968442-82968461 MsG0280011360.01.T01:CDS 10.0%
!! TAATTTAAAAGGTAAAAGTT+GGG + Chr2:82968453-82968472 MsG0280011360.01.T01:CDS 15.0%
!! TTAATTTAAAAGGTAAAAGT+TGG + Chr2:82968452-82968471 MsG0280011360.01.T01:CDS 15.0%
!! TGAATTAAACTGTCAAAATT+GGG - Chr2:82968415-82968434 None:intergenic 20.0%
!! TGCATTTAAATTTAAGGAAT+AGG - Chr2:82968826-82968845 None:intergenic 20.0%
!! TTGAATTAAACTGTCAAAAT+TGG - Chr2:82968416-82968435 None:intergenic 20.0%
!!! TTTTGACAGTTTAATTCAAT+TGG + Chr2:82968417-82968436 MsG0280011360.01.T01:CDS 20.0%
! AATGTTTCGTCTATAGAAAT+TGG + Chr2:82968329-82968348 MsG0280011360.01.T01:intron 25.0%
! ATGTGAGATATAATTGAATC+TGG - Chr2:82967667-82967686 None:intergenic 25.0%
! ATGTTTCGTCTATAGAAATT+GGG + Chr2:82968330-82968349 MsG0280011360.01.T01:intron 25.0%
! GAATTAAACTGTCAAAATTG+GGG - Chr2:82968414-82968433 None:intergenic 25.0%
! GTATAGCTGCATTAATAAAA+CGG - Chr2:82967916-82967935 None:intergenic 25.0%
! GTGCTTTGCATTTAAATTTA+AGG - Chr2:82968832-82968851 None:intergenic 25.0%
! TAATTCACTTGATTGTTTAC+AGG + Chr2:82968620-82968639 MsG0280011360.01.T01:intron 25.0%
! TTGGATAATATGTTGTTCAA+TGG + Chr2:82968097-82968116 MsG0280011360.01.T01:CDS 25.0%
!! ATGAGGTATTACATATGTTT+AGG + Chr2:82968542-82968561 MsG0280011360.01.T01:intron 25.0%
!!! TCATGAATATATTGACTTTC+AGG - Chr2:82968247-82968266 None:intergenic 25.0%
AAACAGAGAACTTTGAGTAT+AGG - Chr2:82968079-82968098 None:intergenic 30.0%
AAGAGAATCTTGTCAATATC+TGG - Chr2:82968127-82968146 None:intergenic 30.0%
AATTTATGAGGACAATTGGA+AGG + Chr2:82968656-82968675 MsG0280011360.01.T01:CDS 30.0%
ATGGAATTTATGAGGACAAT+TGG + Chr2:82968652-82968671 MsG0280011360.01.T01:CDS 30.0%
ATTAATGCAGCTATACAAAG+AGG + Chr2:82967920-82967939 MsG0280011360.01.T01:CDS 30.0%
CAAAGATACATCGATAATAG+AGG - Chr2:82968598-82968617 None:intergenic 30.0%
CATATGTAATACCTCATGAA+TGG - Chr2:82968539-82968558 None:intergenic 30.0%
CATATTCTATGCCAAATTTG+AGG - Chr2:82967877-82967896 None:intergenic 30.0%
CATCGAAAATGGAATTTATG+AGG + Chr2:82968644-82968663 MsG0280011360.01.T01:CDS 30.0%
GATATTGACAAGATTCTCTT+AGG + Chr2:82968127-82968146 MsG0280011360.01.T01:CDS 30.0%
TATACTCAAAGTTCTCTGTT+TGG + Chr2:82968078-82968097 MsG0280011360.01.T01:CDS 30.0%
! AGAGAATATGCGTTTAAAAC+TGG + Chr2:82968285-82968304 MsG0280011360.01.T01:CDS 30.0%
!! AATACTTGTTATCGCCAAAT+TGG - Chr2:82968224-82968243 None:intergenic 30.0%
!! ATACTTGTTATCGCCAAATT+GGG - Chr2:82968223-82968242 None:intergenic 30.0%
AATGGTAAGATGTTGAAGCT+TGG - Chr2:82968521-82968540 None:intergenic 35.0%
AATTGTCGTCTTTCACAGAT+AGG - Chr2:82968042-82968061 None:intergenic 35.0%
AGCTGCATTAATAAAACGGT+TGG - Chr2:82967912-82967931 None:intergenic 35.0%
ATCCATTCCAACAGAAGATA+GGG + Chr2:82967630-82967649 MsG0280011360.01.T01:CDS 35.0%
ATGCAAAGCACGAAATGTTA+CGG + Chr2:82968841-82968860 MsG0280011360.01.T01:CDS 35.0%
ATGGTAAGATGTTGAAGCTT+GGG - Chr2:82968520-82968539 None:intergenic 35.0%
GACATGTTCGAGAAGTTTAA+TGG + Chr2:82967962-82967981 MsG0280011360.01.T01:CDS 35.0%
GTAAAAGTTGGGATAAGAAG+TGG + Chr2:82968464-82968483 MsG0280011360.01.T01:CDS 35.0%
TCAACATCTTACCATTCATG+AGG + Chr2:82968525-82968544 MsG0280011360.01.T01:CDS 35.0%
TCATGAATACTTCCTACCTA+GGG + Chr2:82968261-82968280 MsG0280011360.01.T01:CDS 35.0%
TCGTCTATAGAAATTGGGTA+TGG + Chr2:82968335-82968354 MsG0280011360.01.T01:intron 35.0%
TGATACAATGACCGAGTATA+TGG - Chr2:82967822-82967841 None:intergenic 35.0%
TGTTTACAGGACATCGAAAA+TGG + Chr2:82968633-82968652 MsG0280011360.01.T01:intron 35.0%
TTCATGAATACTTCCTACCT+AGG + Chr2:82968260-82968279 MsG0280011360.01.T01:CDS 35.0%
! CTAAGAACTTGCTTGTTTAC+AGG + Chr2:82968714-82968733 MsG0280011360.01.T01:CDS 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG - Chr2:82968158-82968177 None:intergenic 35.0%
!! GTGATGTGTCTTTGAAGTTT+GGG - Chr2:82967788-82967807 None:intergenic 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG - Chr2:82968157-82968176 None:intergenic 35.0%
!! TTTGCAATTCCTCTAGAATG+GGG - Chr2:82968156-82968175 None:intergenic 35.0%
AAACGCATATTCTCTACCCT+AGG - Chr2:82968280-82968299 None:intergenic 40.0%
AAATTATCTGTGTGCCCAAG+GGG + Chr2:82968864-82968883 MsG0280011360.01.T01:CDS 40.0%
AACCCTATCTTCTGTTGGAA+TGG - Chr2:82967635-82967654 None:intergenic 40.0%
AATATAAGTTCACAGCCCCT+TGG - Chr2:82968882-82968901 None:intergenic 40.0%
AGCTGAGATGAAATGCATTC+CGG - Chr2:82968696-82968715 None:intergenic 40.0%
ATATAAGTTCACAGCCCCTT+GGG - Chr2:82968881-82968900 None:intergenic 40.0%
CGATCATTTCGCCTCAAATT+TGG + Chr2:82967863-82967882 MsG0280011360.01.T01:CDS 40.0%
CTTGCAACTAAAAATGCGAC+TGG - Chr2:82967993-82968012 None:intergenic 40.0%
GAAATTATCTGTGTGCCCAA+GGG + Chr2:82968863-82968882 MsG0280011360.01.T01:CDS 40.0%
GATCCATTCCAACAGAAGAT+AGG + Chr2:82967629-82967648 MsG0280011360.01.T01:CDS 40.0%
TTGAGGCGAAATGATCGTAT+TGG - Chr2:82967860-82967879 None:intergenic 40.0%
! ATAGGCACTGTGAATTGTCA+TGG - Chr2:82968808-82968827 None:intergenic 40.0%
!! GGTGATGTGTCTTTGAAGTT+TGG - Chr2:82967789-82967808 None:intergenic 40.0%
AATGGATCGATGAGGTTGAG+AGG - Chr2:82967617-82967636 None:intergenic 45.0%
ATGCATTCCGGAGCAATATC+TGG - Chr2:82968684-82968703 None:intergenic 45.0%
CAATGACCGAGTATATGGCA+TGG - Chr2:82967817-82967836 None:intergenic 45.0%
CGAAACGGTGGAATCCATTA+TGG + Chr2:82967734-82967753 MsG0280011360.01.T01:CDS 45.0%
CTTTCGCCATGCCATATACT+CGG + Chr2:82967808-82967827 MsG0280011360.01.T01:CDS 45.0%
GAGCTAACCCTATCTTCTGT+TGG - Chr2:82967640-82967659 None:intergenic 45.0%
GCATATTCTCTACCCTAGGT+AGG - Chr2:82968276-82968295 None:intergenic 45.0%
GGAAATTATCTGTGTGCCCA+AGG + Chr2:82968862-82968881 MsG0280011360.01.T01:CDS 45.0%
GTGGAATCCATTATGGCTCT+CGG + Chr2:82967741-82967760 MsG0280011360.01.T01:CDS 45.0%
GTTGGGATAAGAAGTGGAAG+TGG + Chr2:82968470-82968489 MsG0280011360.01.T01:CDS 45.0%
TACTCCAGAAACGCCCAATT+TGG + Chr2:82968207-82968226 MsG0280011360.01.T01:CDS 45.0%
TCTGTTGGAATGGATCGATG+AGG - Chr2:82967625-82967644 None:intergenic 45.0%
TGCATTCCGGAGCAATATCT+GGG - Chr2:82968683-82968702 None:intergenic 45.0%
ATCGCCAAATTGGGCGTTTC+TGG - Chr2:82968214-82968233 None:intergenic 50.0%
ATGGATTCCACCGTTTCGAG+AGG - Chr2:82967732-82967751 None:intergenic 50.0%
CGAGTATATGGCATGGCGAA+AGG - Chr2:82967810-82967829 None:intergenic 50.0%
GAAGGACCCAGATATTGCTC+CGG + Chr2:82968674-82968693 MsG0280011360.01.T01:CDS 50.0%
TCGAGAACCGAGAGCCATAA+TGG - Chr2:82967751-82967770 None:intergenic 50.0%
TGCTTGTTTACAGGCTGCAG+AGG + Chr2:82968723-82968742 MsG0280011360.01.T01:CDS 50.0%
CCACTAGCGTCCTCTCGAAA+CGG + Chr2:82967719-82967738 MsG0280011360.01.T01:CDS 55.0%
CTTAGGCTGCCCCATTCTAG+AGG + Chr2:82968144-82968163 MsG0280011360.01.T01:CDS 55.0%
CCGTTTCGAGAGGACGCTAG+TGG - Chr2:82967722-82967741 None:intergenic 60.0%
CTAGCGTCCTCTCGAAACGG+TGG + Chr2:82967722-82967741 MsG0280011360.01.T01:CDS 60.0%
! GCTAGTGGCGACGGAGTGTT+TGG - Chr2:82967707-82967726 None:intergenic 60.0%
! GTGGCGACGGAGTGTTTGGT+TGG - Chr2:82967703-82967722 None:intergenic 60.0%
CGAGAGGACGCTAGTGGCGA+CGG - Chr2:82967716-82967735 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 82967608 82968908 82967608 ID=MsG0280011360.01;Name=MsG0280011360.01
Chr2 mRNA 82967608 82968908 82967608 ID=MsG0280011360.01.T01;Parent=MsG0280011360.01;Name=MsG0280011360.01.T01;_AED=0.34;_eAED=0.43;_QI=0|0|0|1|1|1|3|0|374
Chr2 exon 82967608 82968306 82967608 ID=MsG0280011360.01.T01:exon:12604;Parent=MsG0280011360.01.T01
Chr2 exon 82968388 82968546 82968388 ID=MsG0280011360.01.T01:exon:12605;Parent=MsG0280011360.01.T01
Chr2 exon 82968642 82968908 82968642 ID=MsG0280011360.01.T01:exon:12606;Parent=MsG0280011360.01.T01
Chr2 CDS 82967608 82968306 82967608 ID=MsG0280011360.01.T01:cds;Parent=MsG0280011360.01.T01
Chr2 CDS 82968388 82968546 82968388 ID=MsG0280011360.01.T01:cds;Parent=MsG0280011360.01.T01
Chr2 CDS 82968642 82968908 82968642 ID=MsG0280011360.01.T01:cds;Parent=MsG0280011360.01.T01
Gene Sequence

>MsG0280011360.01.T01

ATGTCTCCTCTCAACCTCATCGATCCATTCCAACAGAAGATAGGGTTAGCTCTCTTCCAGATTCAATTATATCTCACATTCTCTCTTTTCTTCCAACCAAACACTCCGTCGCCACTAGCGTCCTCTCGAAACGGTGGAATCCATTATGGCTCTCGGTTCTCGATCTCAACTTTGATGCCCAAACTTCAAAGACACATCACCTTTCGCCATGCCATATACTCGGTCATTGTATCACGAGACAACACGCTGCCAATACGATCATTTCGCCTCAAATTTGGCATAGAATATGACATCAACGACGCCAACCGTTTTATTAATGCAGCTATACAAAGAGGAATTGAAAAGCTAGACATTGACATGTTCGAGAAGTTTAATGGATTAACCAGTCGCATTTTTAGTTGCAAGACACTCACTGTTCTCAAGTTGAAAAACCTATCTGTGAAAGACGACAATTGTAATGTAGATCTTCCTATACTCAAAGTTCTCTGTTTGGATAATATGTTGTTCAATGGTAAACCAGATATTGACAAGATTCTCTTAGGCTGCCCCATTCTAGAGGAATTGCAAACAAGTCTTGCTGCATTATATTTAAAGAAGCTTACTCCAGAAACGCCCAATTTGGCGATAACAAGTATTCCTGAAAGTCAATATATTCATGAATACTTCCTACCTAGGGTAGAGAATATGCGTTTAAAACTGAAACTTCACATGTACTTACTAGTCCCCAATTTTGACAGTTTAATTCAATTGGAGCTATTTTTCTTTAATTTAAAAGGTAAAAGTTGGGATAAGAAGTGGAAGTGGATGCTAGCAGTGCTTCAACATAGTCCCAAGCTTCAACATCTTACCATTCATGAGGACATCGAAAATGGAATTTATGAGGACAATTGGAAGGACCCAGATATTGCTCCGGAATGCATTTCATCTCAGCTAAGAACTTGCTTGTTTACAGGCTGCAGAGGCAGAAAGTCTGAGCTTCAATTTGCAGAGTATCTTATGAAAAATTCAAAAGTGTTACGTACCATGACAATTCACAGTGCCTATTCCTTAAATTTAAATGCAAAGCACGAAATGTTACGGAAATTATCTGTGTGCCCAAGGGGCTGTGAACTTATATTTGATTGA
Protein sequence

>MsG0280011360.01.T01

MSPLNLIDPFQQKIGLALFQIQLYLTFSLFFQPNTPSPLASSRNGGIHYGSRFSISTLMPKLQRHITFRHAIYSVIVSRDNTLPIRSFRLKFGIEYDINDANRFINAAIQRGIEKLDIDMFEKFNGLTSRIFSCKTLTVLKLKNLSVKDDNCNVDLPILKVLCLDNMLFNGKPDIDKILLGCPILEELQTSLAALYLKKLTPETPNLAITSIPESQYIHEYFLPRVENMRLKLKLHMYLLVPNFDSLIQLELFFFNLKGKSWDKKWKWMLAVLQHSPKLQHLTIHEDIENGIYEDNWKDPDIAPECISSQLRTCLFTGCRGRKSELQFAEYLMKNSKVLRTMTIHSAYSLNLNAKHEMLRKLSVCPRGCELIFD*