AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012970.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012970.01.T01 MTR_7g098510 47.352 321 144 12 1 306 81 391 3.93e-80 248
MsG0380012970.01.T01 MTR_7g098570 45.631 309 157 5 1 301 83 388 9.58e-76 240
MsG0380012970.01.T01 MTR_7g115270 44.037 327 155 7 2 303 76 399 4.39e-71 225
MsG0380012970.01.T01 MTR_3g036250 44.089 313 148 8 1 303 68 363 7.16e-71 224
MsG0380012970.01.T01 MTR_7g098480 43.871 310 151 8 4 300 84 383 8.23e-71 227
MsG0380012970.01.T01 MTR_1g041245 44.479 317 161 8 1 306 74 386 8.37e-71 224
MsG0380012970.01.T01 MTR_7g056433 43.168 322 158 12 2 306 76 389 3.58e-66 212
MsG0380012970.01.T01 MTR_7g034120 41.379 319 157 13 1 303 1 305 4.09e-64 204
MsG0380012970.01.T01 MTR_7g088260 40.566 318 164 9 1 304 154 460 2.41e-63 207
MsG0380012970.01.T01 MTR_3g040700 42.545 275 136 5 1 266 106 367 2.98e-62 204
MsG0380012970.01.T01 MTR_7g032380 40.064 312 146 9 4 306 77 356 7.12e-62 200
MsG0380012970.01.T01 MTR_0543s0010 40.523 306 170 8 3 304 151 448 3.64e-60 198
MsG0380012970.01.T01 MTR_7g034115 40.256 313 161 11 1 298 74 375 5.36e-59 193
MsG0380012970.01.T01 MTR_7g032370 38.462 312 168 8 4 306 56 352 1.58e-57 189
MsG0380012970.01.T01 MTR_7g033915 39.432 317 150 10 1 303 74 362 6.05e-57 187
MsG0380012970.01.T01 MTR_0492s0020 40.273 293 163 8 3 291 151 435 1.02e-56 189
MsG0380012970.01.T01 MTR_7g033990 41.066 319 154 11 1 300 74 377 1.13e-56 188
MsG0380012970.01.T01 MTR_3g023630 38.762 307 173 7 4 304 75 372 2.38e-56 186
MsG0380012970.01.T01 MTR_3g082890 39.275 331 169 10 1 304 157 482 1.11e-54 185
MsG0380012970.01.T01 MTR_5g013500 38.629 321 167 10 2 306 59 365 2.62e-53 178
MsG0380012970.01.T01 MTR_3g108150 39.809 314 171 7 3 304 78 385 3.80e-53 178
MsG0380012970.01.T01 MTR_3g108560 37.380 313 179 8 1 304 83 387 6.09e-53 178
MsG0380012970.01.T01 MTR_3g105060 38.188 309 169 7 4 306 81 373 8.72e-52 174
MsG0380012970.01.T01 MTR_3g467400 38.365 318 150 10 9 306 76 367 1.89e-51 174
MsG0380012970.01.T01 MTR_3g082890 34.783 368 171 10 1 304 77 439 7.89e-50 171
MsG0380012970.01.T01 MTR_5g008950 37.091 275 160 8 38 306 98 365 1.24e-49 169
MsG0380012970.01.T01 MTR_3g087260 40.364 275 153 6 38 304 184 455 1.39e-48 168
MsG0380012970.01.T01 MTR_3g087260 45.918 98 47 2 25 117 83 179 3.61e-15 75.9
MsG0380012970.01.T01 MTR_5g008920 35.032 314 190 7 1 306 74 381 4.87e-48 165
MsG0380012970.01.T01 MTR_7g114710 40.252 318 142 12 4 306 80 364 5.88e-46 159
MsG0380012970.01.T01 MTR_7g056420 39.550 311 148 9 4 300 75 359 1.47e-44 162
MsG0380012970.01.T01 MTR_7g056430 39.550 311 148 9 4 300 75 359 1.71e-44 162
MsG0380012970.01.T01 MTR_5g012840 35.220 318 143 10 1 306 52 318 5.10e-44 153
MsG0380012970.01.T01 MTR_3g107160 34.722 288 166 9 30 306 107 383 2.06e-42 150
MsG0380012970.01.T01 MTR_3g109982 40.551 254 142 7 51 299 220 469 3.53e-41 152
MsG0380012970.01.T01 MTR_3g109982 45.833 120 59 3 1 116 76 193 2.19e-20 92.0
MsG0380012970.01.T01 MTR_3g107100 35.216 301 162 13 28 303 103 395 1.62e-38 140
MsG0380012970.01.T01 MTR_2g020180 38.222 225 109 7 98 306 5 215 1.68e-38 135
MsG0380012970.01.T01 MTR_3g036200 76.596 94 6 1 1 94 1 78 3.89e-38 131
MsG0380012970.01.T01 MTR_7g056020 38.112 286 154 9 29 300 92 368 8.87e-38 142
MsG0380012970.01.T01 MTR_3g107120 35.315 286 165 9 28 304 115 389 1.67e-36 135
MsG0380012970.01.T01 MTR_7g056277 34.106 302 181 9 9 303 335 625 7.13e-36 136
MsG0380012970.01.T01 MTR_1g085120 32.143 308 190 9 9 303 167 468 1.47e-35 134
MsG0380012970.01.T01 MTR_7g056287 33.770 305 178 10 9 303 79 369 1.95e-35 131
MsG0380012970.01.T01 MTR_1g085120 32.143 308 190 9 9 303 242 543 3.35e-35 134
MsG0380012970.01.T01 MTR_6g040270 32.667 300 185 9 13 303 87 378 8.61e-35 130
MsG0380012970.01.T01 MTR_7g034110 33.562 292 135 8 10 288 80 325 1.26e-34 129
MsG0380012970.01.T01 MTR_1g107385 32.463 268 155 6 50 303 165 420 1.13e-32 125
MsG0380012970.01.T01 MTR_2g005990 29.836 305 178 9 23 303 100 392 6.42e-32 122
MsG0380012970.01.T01 MTR_7g060470 33.209 268 144 8 37 280 110 366 1.21e-31 122
MsG0380012970.01.T01 MTR_7g007080 33.562 292 165 11 25 303 101 376 1.25e-31 122
MsG0380012970.01.T01 MTR_5g058000 31.104 299 193 7 9 303 83 372 3.43e-31 120
MsG0380012970.01.T01 MTR_7g081290 41.758 182 77 7 146 304 79 254 4.96e-31 117
MsG0380012970.01.T01 MTR_1g053025 29.630 297 174 10 30 304 104 387 5.17e-31 120
MsG0380012970.01.T01 MTR_1089s0010 32.558 301 188 8 10 303 84 376 7.62e-31 119
MsG0380012970.01.T01 MTR_6g022500 31.329 316 178 8 9 303 176 473 1.39e-30 120
MsG0380012970.01.T01 MTR_5g007410 30.645 310 191 11 12 304 104 406 2.09e-30 119
MsG0380012970.01.T01 MTR_5g058100 30.619 307 192 9 9 303 84 381 3.76e-30 117
MsG0380012970.01.T01 MTR_7g055943 32.039 309 191 9 9 303 115 418 9.00e-30 117
MsG0380012970.01.T01 MTR_5g073420 32.283 254 156 7 24 273 103 344 1.40e-29 116
MsG0380012970.01.T01 MTR_7g026500 28.254 315 197 8 9 304 89 393 5.50e-29 114
MsG0380012970.01.T01 MTR_4g080610 36.681 229 127 7 78 294 124 346 6.05e-29 114
MsG0380012970.01.T01 MTR_1g108800 34.641 306 171 12 10 303 51 339 8.10e-29 113
MsG0380012970.01.T01 MTR_5g045170 31.724 290 175 9 29 303 107 388 1.44e-28 113
MsG0380012970.01.T01 MTR_2g008320 33.209 268 154 9 51 306 173 427 1.67e-28 114
MsG0380012970.01.T01 MTR_1g108820 30.820 305 182 10 19 303 88 383 2.52e-28 112
MsG0380012970.01.T01 MTR_1g066830 30.662 287 181 8 29 303 104 384 2.70e-28 112
MsG0380012970.01.T01 MTR_2g007450 29.524 315 172 8 27 303 115 417 4.46e-28 112
MsG0380012970.01.T01 MTR_4g039440 29.503 322 201 12 1 303 95 409 4.51e-28 112
MsG0380012970.01.T01 MTR_6g011460 30.599 317 189 13 4 303 116 418 4.66e-28 112
MsG0380012970.01.T01 MTR_6g045333 32.692 312 181 11 12 303 141 443 7.22e-28 112
MsG0380012970.01.T01 MTR_2g007360 30.841 321 170 14 14 303 72 371 8.78e-28 111
MsG0380012970.01.T01 MTR_7g007340 34.962 266 145 10 46 300 103 351 1.37e-27 110
MsG0380012970.01.T01 MTR_0009s0080 33.333 267 158 7 29 281 115 375 5.71e-27 109
MsG0380012970.01.T01 MTR_3g071850 31.210 314 181 11 12 302 104 405 9.85e-27 110
MsG0380012970.01.T01 MTR_5g063950 30.721 319 196 11 1 304 52 360 1.76e-26 107
MsG0380012970.01.T01 MTR_2g007400 29.412 306 172 13 27 303 110 400 3.89e-26 107
MsG0380012970.01.T01 MTR_6g022360 28.065 310 213 6 2 304 100 406 5.26e-26 106
MsG0380012970.01.T01 MTR_7g083740 29.568 301 177 13 19 304 85 365 6.55e-26 105
MsG0380012970.01.T01 MTR_6g022520 32.075 318 193 10 1 303 1 310 1.00e-25 104
MsG0380012970.01.T01 MTR_4g098590 31.387 274 153 11 27 276 95 357 1.91e-25 104
MsG0380012970.01.T01 MTR_3g099830 29.642 307 179 10 19 303 80 371 9.05e-25 102
MsG0380012970.01.T01 MTR_5g039450 30.612 294 150 11 29 304 99 356 9.76e-25 102
MsG0380012970.01.T01 MTR_3g115430 44.186 129 65 3 181 306 1 125 1.14e-24 96.7
MsG0380012970.01.T01 MTR_3g108560 34.081 223 130 8 1 214 83 297 4.43e-24 100
MsG0380012970.01.T01 MTR_2g008310 32.210 267 156 11 51 306 119 371 6.72e-24 100
MsG0380012970.01.T01 MTR_8g085430 42.857 126 62 3 173 292 193 314 1.56e-23 99.0
MsG0380012970.01.T01 MTR_8g085430 42.400 125 65 4 1 119 82 205 2.70e-19 87.0
MsG0380012970.01.T01 MTR_7g075820 27.820 266 165 7 50 303 141 391 2.93e-23 99.0
MsG0380012970.01.T01 MTR_5g054920 31.633 196 108 7 103 285 7 189 3.81e-23 94.7
MsG0380012970.01.T01 MTR_5g045510 29.430 316 179 11 3 303 83 369 1.46e-22 96.7
MsG0380012970.01.T01 MTR_5g039470 30.070 286 167 12 3 269 56 327 2.11e-22 95.5
MsG0380012970.01.T01 MTR_5g073370 31.228 285 167 12 10 276 77 350 3.62e-22 95.9
MsG0380012970.01.T01 MTR_1g043170 50.000 86 40 1 221 306 6 88 7.04e-22 88.2
MsG0380012970.01.T01 MTR_7g056417 42.568 148 71 5 49 184 5 150 1.17e-21 89.7
MsG0380012970.01.T01 MTR_3g026920 30.325 277 135 9 50 304 163 403 3.44e-21 93.2
MsG0380012970.01.T01 MTR_2g036470 31.511 311 185 12 12 303 92 393 4.76e-21 92.8
MsG0380012970.01.T01 MTR_3g107940 41.860 129 64 2 174 300 88 207 6.85e-21 91.7
MsG0380012970.01.T01 MTR_5g031970 29.276 304 183 10 8 303 74 353 9.56e-21 91.3
MsG0380012970.01.T01 MTR_4g096730 54.878 82 32 2 226 306 12 89 1.84e-20 84.7
MsG0380012970.01.T01 MTR_7g056447 46.491 114 57 2 1 110 72 185 2.04e-20 89.7
MsG0380012970.01.T01 MTR_2g007550 38.686 137 65 3 171 304 213 333 2.97e-20 90.1
MsG0380012970.01.T01 MTR_3g110010 45.833 120 59 3 1 116 76 193 3.60e-20 91.3
MsG0380012970.01.T01 MTR_3g110010 36.310 168 99 6 51 214 220 383 2.94e-15 76.6
MsG0380012970.01.T01 MTR_8g016130 50.459 109 50 3 31 138 57 162 4.04e-20 85.9
MsG0380012970.01.T01 MTR_5g083890 30.612 245 138 8 50 273 141 374 7.35e-20 90.1
MsG0380012970.01.T01 MTR_3g108140 50.633 79 39 0 226 304 68 146 1.51e-19 84.7
MsG0380012970.01.T01 MTR_1377s0010 35.915 142 79 4 149 289 7 137 1.91e-19 83.2
MsG0380012970.01.T01 MTR_4g134150 45.378 119 59 4 1 115 73 189 5.86e-19 84.3
MsG0380012970.01.T01 MTR_5g008910 32.636 239 123 10 96 306 74 302 6.69e-19 85.5
MsG0380012970.01.T01 MTR_5g065100 29.078 282 159 8 26 301 98 344 8.30e-19 86.7
MsG0380012970.01.T01 MTR_7g022105 34.746 236 134 8 51 273 165 393 1.15e-18 86.3
MsG0380012970.01.T01 MTR_3g113770 29.384 211 93 5 54 263 179 334 1.25e-18 85.1
MsG0380012970.01.T01 MTR_7g056280 30.851 188 114 6 98 273 363 546 3.26e-18 85.5
MsG0380012970.01.T01 MTR_6g029350 30.769 208 122 7 103 303 3 195 3.49e-18 82.4
MsG0380012970.01.T01 MTR_3g106960 40.000 135 70 7 148 276 45 174 8.25e-18 81.3
MsG0380012970.01.T01 MTR_2g007500 28.571 266 154 9 13 250 75 332 1.06e-17 82.4
MsG0380012970.01.T01 MTR_8g058965 38.406 138 77 4 171 306 325 456 1.28e-16 80.1
MsG0380012970.01.T01 MTR_7g021990 30.769 234 146 6 51 273 164 392 1.45e-16 80.1
MsG0380012970.01.T01 MTR_7g021950 30.769 234 146 6 51 273 164 392 1.66e-16 80.1
MsG0380012970.01.T01 MTR_1g085120 30.516 213 131 8 9 208 254 462 4.09e-16 79.0
MsG0380012970.01.T01 MTR_5g073340 28.631 241 152 8 25 250 73 308 6.34e-16 77.0
MsG0380012970.01.T01 MTR_8g469020 37.956 137 79 4 171 306 320 451 6.73e-16 78.2
MsG0380012970.01.T01 MTR_8g070950 28.435 313 157 11 49 306 134 434 1.27e-15 77.4
MsG0380012970.01.T01 MTR_5g039490 29.210 291 170 11 20 303 112 373 2.70e-15 75.9
MsG0380012970.01.T01 MTR_1g038710 27.726 321 155 13 27 273 110 427 3.95e-15 75.9
MsG0380012970.01.T01 MTR_5g061680 28.452 239 134 6 9 241 87 294 5.51e-15 74.7
MsG0380012970.01.T01 MTR_7g034090 33.750 160 95 5 148 303 157 309 1.35e-14 74.3
MsG0380012970.01.T01 MTR_8g062010 36.170 141 76 5 171 306 299 430 1.84e-14 73.9
MsG0380012970.01.T01 MTR_2g007610 35.075 134 71 4 174 303 27 148 2.34e-14 70.1
MsG0380012970.01.T01 MTR_3g094980 36.364 121 64 4 178 288 21 138 4.90e-14 69.3
MsG0380012970.01.T01 MTR_7g033945 44.000 100 44 4 1 89 74 172 1.03e-13 68.6
MsG0380012970.01.T01 MTR_8g062310 38.532 109 63 3 197 303 319 425 1.03e-13 71.6
MsG0380012970.01.T01 MTR_8g062300 35.461 141 77 5 171 306 346 477 1.12e-13 71.6
MsG0380012970.01.T01 MTR_5g024060 33.577 137 82 4 171 305 78 207 1.57e-13 69.7
MsG0380012970.01.T01 MTR_5g033200 27.545 167 112 4 133 296 12 172 3.09e-13 67.4
MsG0380012970.01.T01 MTR_2g086020 41.071 112 62 3 197 306 206 315 8.95e-13 68.2
MsG0380012970.01.T01 MTR_8g014160 27.304 293 188 9 28 306 144 425 9.74e-13 68.6
MsG0380012970.01.T01 MTR_7g022060 25.287 261 142 7 47 303 116 327 1.59e-12 67.4
MsG0380012970.01.T01 MTR_3g095630 28.571 147 97 5 129 273 11 151 2.08e-12 65.1
MsG0380012970.01.T01 MTR_7g075800 24.839 310 163 11 33 284 106 403 2.93e-12 67.4
MsG0380012970.01.T01 MTR_2g007530 36.029 136 57 5 171 304 228 335 3.13e-12 66.6
MsG0380012970.01.T01 MTR_7g021930 25.287 261 142 7 47 303 116 327 7.37e-12 65.5
MsG0380012970.01.T01 MTR_7g021970 25.287 261 142 7 47 303 116 327 7.37e-12 65.5
MsG0380012970.01.T01 MTR_3g113785 40.741 81 41 1 190 263 12 92 8.51e-12 61.2
MsG0380012970.01.T01 MTR_7g007370 31.048 248 150 8 50 285 93 331 1.54e-11 64.7
MsG0380012970.01.T01 MTR_8g467800 25.969 258 153 9 60 306 223 453 2.82e-11 64.3
MsG0380012970.01.T01 MTR_8g014210 33.636 110 70 2 197 306 125 231 5.83e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012970.01.T01 AT4G26340 27.246 345 190 10 18 306 80 419 1.10e-22 97.8
MsG0380012970.01.T01 AT3G26920 31.773 299 173 11 27 304 11 299 5.43e-22 94.0
MsG0380012970.01.T01 AT5G56370 27.059 340 185 12 23 304 85 419 4.06e-19 87.4
MsG0380012970.01.T01 AT5G56370 27.059 340 185 12 23 304 85 419 4.06e-19 87.4
MsG0380012970.01.T01 AT3G26920 28.274 336 175 12 27 304 11 338 3.31e-18 84.0
MsG0380012970.01.T01 AT3G26920 28.274 336 175 12 27 304 11 338 3.31e-18 84.0
MsG0380012970.01.T01 AT5G56560 25.602 332 188 8 27 306 94 418 1.43e-15 77.0
MsG0380012970.01.T01 AT1G05080 26.934 349 182 10 21 304 97 437 3.71e-15 75.9
MsG0380012970.01.T01 AT5G44850 27.759 299 177 12 27 304 49 329 9.86e-15 73.9
MsG0380012970.01.T01 AT1G32375 24.781 343 195 10 22 306 85 422 3.79e-14 72.8
MsG0380012970.01.T01 AT1G32375 24.781 343 195 10 22 306 85 422 3.79e-14 72.8
MsG0380012970.01.T01 AT5G56440 24.928 349 187 9 23 303 86 427 2.01e-13 70.9
MsG0380012970.01.T01 AT5G56452 29.121 182 116 5 132 306 52 227 1.01e-12 67.0
MsG0380012970.01.T01 AT5G56452 29.121 182 115 6 132 306 52 226 7.91e-12 64.3
MsG0380012970.01.T01 AT1G78750 23.947 380 181 16 12 304 98 456 9.08e-12 65.9
MsG0380012970.01.T01 AT1G78750 23.947 380 181 16 12 304 98 456 9.08e-12 65.9
MsG0380012970.01.T01 AT5G56560 26.263 297 162 8 60 306 318 607 1.51e-11 65.5
MsG0380012970.01.T01 AT5G56370 39.362 94 51 1 23 110 85 178 2.90e-11 63.9
MsG0380012970.01.T01 AT3G49480 26.316 285 162 7 51 287 1 285 5.02e-11 62.8
MsG0380012970.01.T01 AT5G56420 34.328 134 83 3 174 306 293 422 6.42e-11 63.2
MsG0380012970.01.T01 AT5G56420 34.328 134 83 3 174 306 293 422 6.42e-11 63.2
MsG0380012970.01.T01 AT5G56420 34.328 134 83 3 174 306 293 422 6.42e-11 63.2

Find 50 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAACCAATCCCACATATT+TGG 0.177137 3:+27916058 None:intergenic
TGTTTGCAATTCTTCTAGTT+TGG 0.215830 3:+27916669 None:intergenic
GTATGTGATATTATGAAATA+TGG 0.272821 3:+27916111 None:intergenic
GTTTGCAATTCTTCTAGTTT+GGG 0.275562 3:+27916670 None:intergenic
TTCTTTGGAACAAGTGGATC+TGG 0.334460 3:+27916625 None:intergenic
CTTAGAACATGCAGCCTTAC+GGG 0.335505 3:-27915819 MsG0380012970.01.T01:CDS
TCATACATCTAAGAATACTA+TGG 0.356115 3:+27916833 None:intergenic
TGCAGCCTTACGGGTTATAA+AGG 0.381198 3:-27915810 MsG0380012970.01.T01:CDS
GAAAGACATTTCGGAACAAT+TGG 0.387487 3:+27915849 None:intergenic
TCTTAGATGTATGACACTTA+AGG 0.387856 3:-27916824 MsG0380012970.01.T01:CDS
AGCTGCGATGAAAGACATTT+CGG 0.401646 3:+27915840 None:intergenic
TTAAGATTCGGAAAATCTAC+TGG 0.407136 3:+27916760 None:intergenic
TTAAGGTTCTTAAGTTGAAA+AGG 0.417347 3:-27916807 MsG0380012970.01.T01:CDS
CAAATCGATTAACATCGTAT+TGG 0.428046 3:+27916917 None:intergenic
AAGAGTTATATGACAAAAGT+TGG 0.433730 3:-27915881 MsG0380012970.01.T01:CDS
CTATATACTTTCCAAATATG+TGG 0.443204 3:-27916069 MsG0380012970.01.T01:CDS
ACATTACAAACCAAAGTAAT+CGG 0.447460 3:+27916544 None:intergenic
ACTTTCCAAATATGTGGGAT+TGG 0.447483 3:-27916063 MsG0380012970.01.T01:CDS
TTGCTTTGATTAAATTAGCT+AGG 0.453172 3:+27916590 None:intergenic
GCTTAGAACATGCAGCCTTA+CGG 0.455573 3:-27915820 MsG0380012970.01.T01:CDS
TTCGCTCCCAAGGGGCTCTT+CGG 0.468519 3:-27915661 MsG0380012970.01.T01:CDS
ATTATGAAATATGGGAAGAT+TGG 0.469740 3:+27916120 None:intergenic
CATACATCTAAGAATACTAT+GGG 0.479791 3:+27916834 None:intergenic
TGCTTTGATTAAATTAGCTA+GGG 0.485651 3:+27916591 None:intergenic
TCACAAGATATTCATGACAT+TGG 0.491193 3:+27916710 None:intergenic
TTGAAACGAAATGAAAGGAT+TGG 0.492293 3:+27916964 None:intergenic
TACATGCCGAAGAGCCCCTT+GGG 0.493511 3:+27915655 None:intergenic
TATATACTTTCCAAATATGT+GGG 0.494437 3:-27916068 MsG0380012970.01.T01:CDS
TGCATTTGAAACGAAATGAA+AGG 0.505547 3:+27916959 None:intergenic
AGACATTTCGGAACAATTGG+TGG 0.507203 3:+27915852 None:intergenic
AGCTAATTTAATCAAAGCAA+GGG 0.512686 3:-27916587 MsG0380012970.01.T01:CDS
TAGCTAATTTAATCAAAGCA+AGG 0.523426 3:-27916588 MsG0380012970.01.T01:CDS
CCTTGCCTTTATAACCCGTA+AGG 0.526323 3:+27915805 None:intergenic
ACATCGTATTGGTTGCATGC+AGG 0.535085 3:+27916928 None:intergenic
TGTACTTATCTTCGCTCCCA+AGG 0.541506 3:-27915671 MsG0380012970.01.T01:CDS
GTACTTATCTTCGCTCCCAA+GGG 0.550717 3:-27915670 MsG0380012970.01.T01:CDS
ATTTCATAATATCACATACA+TGG 0.556470 3:-27916107 MsG0380012970.01.T01:CDS
TTTGGAACAAGTGGATCTGG+TGG 0.561311 3:+27916628 None:intergenic
TTACATGCCGAAGAGCCCCT+TGG 0.562732 3:+27915654 None:intergenic
GTTATATGACAAAAGTTGGA+AGG 0.580827 3:-27915877 MsG0380012970.01.T01:CDS
GCGACTACACTTAACTGTCA+TGG 0.587736 3:+27915728 None:intergenic
TATGTGATATTATGAAATAT+GGG 0.589829 3:+27916112 None:intergenic
GCAGAGTCTACGTGTGGACC+TGG 0.594945 3:-27916521 MsG0380012970.01.T01:intron
TTAACTTTGCAATGCAACGA+GGG 0.624674 3:-27916894 MsG0380012970.01.T01:CDS
TACTTATCTTCGCTCCCAAG+GGG 0.657185 3:-27915669 MsG0380012970.01.T01:CDS
CCTTACGGGTTATAAAGGCA+AGG 0.661243 3:-27915805 MsG0380012970.01.T01:CDS
TGTAATTCATGCAGACGACG+TGG 0.665669 3:-27916153 MsG0380012970.01.T01:intron
GTTAACTTTGCAATGCAACG+AGG 0.687499 3:-27916895 MsG0380012970.01.T01:CDS
TAATGTGCAGAGTCTACGTG+TGG 0.708380 3:-27916527 MsG0380012970.01.T01:CDS
ATACATCTAAGAATACTATG+GGG 0.754826 3:+27916835 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATGTACATATGAA+AGG + Chr3:27916410-27916429 None:intergenic 15.0%
!! AAAAAAATGTACATATGAAA+GGG + Chr3:27916409-27916428 None:intergenic 15.0%
!! TATGTGATATTATGAAATAT+GGG + Chr3:27916517-27916536 None:intergenic 15.0%
!! TTTGTCATATAAAGAAAAAT+TGG - Chr3:27916320-27916339 MsG0380012970.01.T01:intron 15.0%
!!! AATTTGCAAAATACATTTTA+CGG - Chr3:27916856-27916875 MsG0380012970.01.T01:CDS 15.0%
!!! TATGTACATTTTTTTTTATC+TGG - Chr3:27916414-27916433 MsG0380012970.01.T01:intron 15.0%
!! AAATTGAATGAATGAATGTA+AGG + Chr3:27916227-27916246 None:intergenic 20.0%
!! AATAGAAACAATTATGTCAA+TGG - Chr3:27916699-27916718 MsG0380012970.01.T01:CDS 20.0%
!! ATTTCAAAACTTAAAAGATG+TGG + Chr3:27916682-27916701 None:intergenic 20.0%
!! ATTTCATAATATCACATACA+TGG - Chr3:27916519-27916538 MsG0380012970.01.T01:intron 20.0%
!! GTATGTGATATTATGAAATA+TGG + Chr3:27916518-27916537 None:intergenic 20.0%
!! TATATACTTTCCAAATATGT+GGG - Chr3:27916558-27916577 MsG0380012970.01.T01:CDS 20.0%
!!! ACTTCAAAATTTTATCATAC+AGG - Chr3:27916615-27916634 MsG0380012970.01.T01:CDS 20.0%
!!! AGATGAAGAATTTTAAGATT+CGG + Chr3:27915881-27915900 None:intergenic 20.0%
!!! ATTACGCTTCTAATTAATTT+TGG - Chr3:27916136-27916155 MsG0380012970.01.T01:CDS 20.0%
!!! GTAAAATGTATTTTGCAAAT+TGG + Chr3:27916857-27916876 None:intergenic 20.0%
!!! TGATAAAATTTTGAAGTTTG+TGG + Chr3:27916612-27916631 None:intergenic 20.0%
! AAGAGTTATATGACAAAAGT+TGG - Chr3:27916745-27916764 MsG0380012970.01.T01:CDS 25.0%
! ACATTACAAACCAAAGTAAT+CGG + Chr3:27916085-27916104 None:intergenic 25.0%
! AGCTAATTTAATCAAAGCAA+GGG - Chr3:27916039-27916058 MsG0380012970.01.T01:CDS 25.0%
! ATACATCTAAGAATACTATG+GGG + Chr3:27915794-27915813 None:intergenic 25.0%
! ATTATGAAATATGGGAAGAT+TGG + Chr3:27916509-27916528 None:intergenic 25.0%
! CATACATCTAAGAATACTAT+GGG + Chr3:27915795-27915814 None:intergenic 25.0%
! CTATATACTTTCCAAATATG+TGG - Chr3:27916557-27916576 MsG0380012970.01.T01:CDS 25.0%
! TAGCTAATTTAATCAAAGCA+AGG - Chr3:27916038-27916057 MsG0380012970.01.T01:CDS 25.0%
! TCATACATCTAAGAATACTA+TGG + Chr3:27915796-27915815 None:intergenic 25.0%
! TGCTTTGATTAAATTAGCTA+GGG + Chr3:27916038-27916057 None:intergenic 25.0%
! TTGCTTTGATTAAATTAGCT+AGG + Chr3:27916039-27916058 None:intergenic 25.0%
! TTGTTCCAAAGAAAAAGTTT+AGG - Chr3:27916011-27916030 MsG0380012970.01.T01:intron 25.0%
!! TCATATTTTTCCGATTACTT+TGG - Chr3:27916072-27916091 MsG0380012970.01.T01:CDS 25.0%
!!! TTAAGGTTCTTAAGTTGAAA+AGG - Chr3:27915819-27915838 MsG0380012970.01.T01:CDS 25.0%
ACAAAATGTTTAGTCCATAG+AGG + Chr3:27916305-27916324 None:intergenic 30.0%
CAAAATGTTTAGTCCATAGA+GGG + Chr3:27916304-27916323 None:intergenic 30.0%
CAAATCGATTAACATCGTAT+TGG + Chr3:27915712-27915731 None:intergenic 30.0%
GTTATATGACAAAAGTTGGA+AGG - Chr3:27916749-27916768 MsG0380012970.01.T01:CDS 30.0%
TCACAAGATATTCATGACAT+TGG + Chr3:27915919-27915938 None:intergenic 30.0%
TCTTAGATGTATGACACTTA+AGG - Chr3:27915802-27915821 MsG0380012970.01.T01:CDS 30.0%
TTAAGATTCGGAAAATCTAC+TGG + Chr3:27915869-27915888 None:intergenic 30.0%
TTGAAACGAAATGAAAGGAT+TGG + Chr3:27915665-27915684 None:intergenic 30.0%
! TGCATTTGAAACGAAATGAA+AGG + Chr3:27915670-27915689 None:intergenic 30.0%
! TGTTTGCAATTCTTCTAGTT+TGG + Chr3:27915960-27915979 None:intergenic 30.0%
!! GTTTGCAATTCTTCTAGTTT+GGG + Chr3:27915959-27915978 None:intergenic 30.0%
!!! AACTTTTTCTTTGGAACAAG+TGG + Chr3:27916010-27916029 None:intergenic 30.0%
ACTTTCCAAATATGTGGGAT+TGG - Chr3:27916563-27916582 MsG0380012970.01.T01:CDS 35.0%
CAATTATGTCAATGGTTGAC+AGG - Chr3:27916707-27916726 MsG0380012970.01.T01:CDS 35.0%
GAAAGACATTTCGGAACAAT+TGG + Chr3:27916780-27916799 None:intergenic 35.0%
GAAGCGTAATATATCATACC+AGG + Chr3:27916126-27916145 None:intergenic 35.0%
TTAACTTTGCAATGCAACGA+GGG - Chr3:27915732-27915751 MsG0380012970.01.T01:CDS 35.0%
!! AGGGTCCTAAACTTTTTCTT+TGG + Chr3:27916019-27916038 None:intergenic 35.0%
!!! TTGTGTTTTTAGTCCCTCTA+TGG - Chr3:27916288-27916307 MsG0380012970.01.T01:intron 35.0%
AGACATTTCGGAACAATTGG+TGG + Chr3:27916777-27916796 None:intergenic 40.0%
AGCAACCAATCCCACATATT+TGG + Chr3:27916571-27916590 None:intergenic 40.0%
AGCTGCGATGAAAGACATTT+CGG + Chr3:27916789-27916808 None:intergenic 40.0%
GTTAACTTTGCAATGCAACG+AGG - Chr3:27915731-27915750 MsG0380012970.01.T01:CDS 40.0%
!! TTCTTTGGAACAAGTGGATC+TGG + Chr3:27916004-27916023 None:intergenic 40.0%
CCTTGCCTTTATAACCCGTA+AGG + Chr3:27916824-27916843 None:intergenic 45.0%
CTTAGAACATGCAGCCTTAC+GGG - Chr3:27916807-27916826 MsG0380012970.01.T01:CDS 45.0%
GCGACTACACTTAACTGTCA+TGG + Chr3:27916901-27916920 None:intergenic 45.0%
GCTTAGAACATGCAGCCTTA+CGG - Chr3:27916806-27916825 MsG0380012970.01.T01:CDS 45.0%
GTACTTATCTTCGCTCCCAA+GGG - Chr3:27916956-27916975 MsG0380012970.01.T01:CDS 45.0%
TAATGTGCAGAGTCTACGTG+TGG - Chr3:27916099-27916118 MsG0380012970.01.T01:CDS 45.0%
TACTTATCTTCGCTCCCAAG+GGG - Chr3:27916957-27916976 MsG0380012970.01.T01:CDS 45.0%
TGTAATTCATGCAGACGACG+TGG - Chr3:27916473-27916492 MsG0380012970.01.T01:intron 45.0%
TGTACTTATCTTCGCTCCCA+AGG - Chr3:27916955-27916974 MsG0380012970.01.T01:CDS 45.0%
! CCTTACGGGTTATAAAGGCA+AGG - Chr3:27916821-27916840 MsG0380012970.01.T01:CDS 45.0%
! TGCAGCCTTACGGGTTATAA+AGG - Chr3:27916816-27916835 MsG0380012970.01.T01:CDS 45.0%
! TTTGGAACAAGTGGATCTGG+TGG + Chr3:27916001-27916020 None:intergenic 45.0%
!! ACATCGTATTGGTTGCATGC+AGG + Chr3:27915701-27915720 None:intergenic 45.0%
TACATGCCGAAGAGCCCCTT+GGG + Chr3:27916974-27916993 None:intergenic 55.0%
TTACATGCCGAAGAGCCCCT+TGG + Chr3:27916975-27916994 None:intergenic 55.0%
GCAGAGTCTACGTGTGGACC+TGG - Chr3:27916105-27916124 MsG0380012970.01.T01:CDS 60.0%
TTCGCTCCCAAGGGGCTCTT+CGG - Chr3:27916965-27916984 MsG0380012970.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 27915638 27917010 27915638 ID=MsG0380012970.01;Name=MsG0380012970.01
Chr3 mRNA 27915638 27917010 27915638 ID=MsG0380012970.01.T01;Parent=MsG0380012970.01;Name=MsG0380012970.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|0.66|3|0|306
Chr3 exon 27916522 27917010 27916522 ID=MsG0380012970.01.T01:exon:7453;Parent=MsG0380012970.01.T01
Chr3 exon 27916012 27916161 27916012 ID=MsG0380012970.01.T01:exon:7454;Parent=MsG0380012970.01.T01
Chr3 exon 27915638 27915919 27915638 ID=MsG0380012970.01.T01:exon:7455;Parent=MsG0380012970.01.T01
Chr3 CDS 27916522 27917010 27916522 ID=MsG0380012970.01.T01:cds;Parent=MsG0380012970.01.T01
Chr3 CDS 27916012 27916161 27916012 ID=MsG0380012970.01.T01:cds;Parent=MsG0380012970.01.T01
Chr3 CDS 27915638 27915919 27915638 ID=MsG0380012970.01.T01:cds;Parent=MsG0380012970.01.T01
Gene Sequence

>MsG0380012970.01.T01

ATGATCTCACGAAACATCACACTACCAATCCTTTCATTTCGTTTCAAATGCAACTCTTCTCCTGCATGCAACCAATACGATGTTAATCGATTTGTTAACTTTGCAATGCAACGAGGGATTGAAAATCTCAGTATAAGCATTGACATCAAATTACCCCATAGTATTCTTAGATGTATGACACTTAAGGTTCTTAAGTTGAAAAGGATAACAGTGAAAGATCTTTCTCATCCAGTAGATTTTCCGAATCTTAAAATTCTTCATCTGAAGACTGTGTTTTTCCAATGTCATGAATATCTTGTGAATTTTCTATCTAGTTGTCCCAAACTAGAAGAATTGCAAACAAAAGATTTACGTGTAGTTCCACCAGATCCACTTGTTCCAAAGAAAAAGTTTAGGACCCTAGCTAATTTAATCAAAGCAAGGGTTTTTGAAGATCATATTTTTCCGATTACTTTGGTTTGTAATGTGCAGAGTCTACGTGTGGACCTGACGACGTGGATACGATGCACCAATCTTCCCATATTTCATAATATCACATACATGGAGCTTATATTTAGTTCTATATACTTTCCAAATATGTGGGATTGGTTGCTACAAGTGCTTGATCGTTGCCACAAACTTCAAAATTTTATCATACAGGATTGCATATATCGAGAAGAGTTATATGACAAAAGTTGGAAGGATCCACCAATTGTTCCGAAATGTCTTTCATCGCAGCTTAGAACATGCAGCCTTACGGGTTATAAAGGCAAGGAACTTGAGCTCCAATTTGCAAAATACATTTTACGGAATTCAAAAGTACTGCAGACCATGACAGTTAAGTGTAGTCGCTTAATAGATTTAAATGCAAAGCAGCAAATGTTAATGTACTTATCTTCGCTCCCAAGGGGCTCTTCGGCATGTAAACTTTTATCTGATTGA
Protein sequence

>MsG0380012970.01.T01

MISRNITLPILSFRFKCNSSPACNQYDVNRFVNFAMQRGIENLSISIDIKLPHSILRCMTLKVLKLKRITVKDLSHPVDFPNLKILHLKTVFFQCHEYLVNFLSSCPKLEELQTKDLRVVPPDPLVPKKKFRTLANLIKARVFEDHIFPITLVCNVQSLRVDLTTWIRCTNLPIFHNITYMELIFSSIYFPNMWDWLLQVLDRCHKLQNFIIQDCIYREELYDKSWKDPPIVPKCLSSQLRTCSLTGYKGKELELQFAKYILRNSKVLQTMTVKCSRLIDLNAKQQMLMYLSSLPRGSSACKLLSD*