AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037551.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037551.01.T01 MTR_7g033915 74.920 311 57 4 1 309 74 365 7.71e-165 463
MsG0780037551.01.T01 MTR_7g033990 80.064 311 55 5 1 307 74 381 1.86e-164 464
MsG0780037551.01.T01 MTR_7g034120 58.413 315 116 7 1 308 1 307 1.07e-117 341
MsG0780037551.01.T01 MTR_1g041245 50.155 323 135 10 1 308 74 385 2.83e-91 277
MsG0780037551.01.T01 MTR_7g034115 48.232 311 142 7 1 301 74 375 8.76e-87 265
MsG0780037551.01.T01 MTR_5g012840 46.474 312 117 6 1 308 52 317 1.28e-81 249
MsG0780037551.01.T01 MTR_7g032380 44.300 307 128 8 10 308 84 355 3.85e-79 244
MsG0780037551.01.T01 MTR_7g032370 42.138 318 149 8 4 308 56 351 2.51e-70 222
MsG0780037551.01.T01 MTR_7g098510 42.508 327 152 13 1 308 81 390 5.60e-64 207
MsG0780037551.01.T01 MTR_7g056433 42.056 321 166 11 1 308 75 388 9.61e-63 204
MsG0780037551.01.T01 MTR_7g115270 39.877 326 175 9 1 306 75 399 6.39e-62 202
MsG0780037551.01.T01 MTR_0543s0010 41.270 315 160 11 4 309 152 450 1.18e-60 200
MsG0780037551.01.T01 MTR_7g034110 41.837 294 126 4 10 299 80 332 1.18e-59 194
MsG0780037551.01.T01 MTR_7g088260 40.244 328 156 12 1 308 154 461 1.42e-57 192
MsG0780037551.01.T01 MTR_3g108150 39.628 323 164 10 4 309 79 387 2.70e-57 189
MsG0780037551.01.T01 MTR_5g013500 38.739 333 153 13 1 308 58 364 5.51e-56 185
MsG0780037551.01.T01 MTR_7g098570 40.881 318 162 11 1 304 83 388 6.62e-56 188
MsG0780037551.01.T01 MTR_3g108560 38.318 321 172 10 1 309 83 389 1.72e-54 182
MsG0780037551.01.T01 MTR_7g098480 41.176 323 156 14 4 309 84 389 8.58e-54 182
MsG0780037551.01.T01 MTR_0492s0020 40.397 302 155 11 4 296 152 437 8.60e-54 181
MsG0780037551.01.T01 MTR_3g082890 39.244 344 156 14 1 308 157 483 4.79e-51 176
MsG0780037551.01.T01 MTR_3g087260 40.956 293 133 12 38 309 184 457 1.80e-50 173
MsG0780037551.01.T01 MTR_3g036250 35.826 321 166 11 1 306 68 363 6.46e-49 167
MsG0780037551.01.T01 MTR_7g056420 38.871 319 148 10 1 303 72 359 3.40e-48 173
MsG0780037551.01.T01 MTR_7g056430 38.871 319 148 10 1 303 72 359 3.52e-48 173
MsG0780037551.01.T01 MTR_8g085430 35.906 298 123 5 3 297 84 316 2.91e-47 162
MsG0780037551.01.T01 MTR_5g008950 37.383 321 158 13 10 308 65 364 2.36e-46 160
MsG0780037551.01.T01 MTR_3g082890 34.383 381 160 15 1 308 77 440 5.13e-46 161
MsG0780037551.01.T01 MTR_7g026500 35.110 319 175 11 9 308 89 394 4.54e-44 155
MsG0780037551.01.T01 MTR_3g109982 42.126 254 125 11 65 302 222 469 4.61e-43 157
MsG0780037551.01.T01 MTR_3g109982 32.174 230 116 11 1 215 76 280 1.69e-13 71.2
MsG0780037551.01.T01 MTR_5g008920 34.049 326 178 11 1 308 74 380 1.45e-41 148
MsG0780037551.01.T01 MTR_3g071850 35.220 318 170 12 10 305 102 405 5.05e-41 152
MsG0780037551.01.T01 MTR_6g045333 35.093 322 173 12 10 309 139 446 5.44e-40 145
MsG0780037551.01.T01 MTR_7g056277 34.967 306 180 8 9 308 335 627 8.37e-40 147
MsG0780037551.01.T01 MTR_7g056287 33.333 306 185 7 9 308 79 371 3.11e-39 142
MsG0780037551.01.T01 MTR_5g007410 33.333 315 181 9 10 306 102 405 5.59e-39 142
MsG0780037551.01.T01 MTR_7g056020 34.936 312 175 9 3 303 74 368 5.73e-39 145
MsG0780037551.01.T01 MTR_3g040700 35.315 286 151 9 1 273 106 370 1.16e-38 142
MsG0780037551.01.T01 MTR_3g023630 33.227 313 183 11 4 306 75 371 1.57e-37 137
MsG0780037551.01.T01 MTR_3g105060 32.907 313 181 10 4 308 81 372 1.67e-37 137
MsG0780037551.01.T01 MTR_3g467400 35.962 317 160 13 9 308 76 366 1.89e-37 137
MsG0780037551.01.T01 MTR_7g033945 61.468 109 39 2 1 108 74 180 5.37e-37 130
MsG0780037551.01.T01 MTR_6g022500 34.603 315 174 12 9 307 176 474 3.39e-36 135
MsG0780037551.01.T01 MTR_5g045170 33.951 324 177 11 8 309 83 391 5.55e-36 134
MsG0780037551.01.T01 MTR_1g066830 32.188 320 179 11 10 308 84 386 6.04e-35 130
MsG0780037551.01.T01 MTR_3g107100 32.899 307 166 11 28 307 103 396 2.22e-34 129
MsG0780037551.01.T01 MTR_5g058100 33.648 318 175 12 9 308 84 383 2.46e-34 129
MsG0780037551.01.T01 MTR_7g081290 50.000 138 59 5 177 308 122 255 3.60e-34 125
MsG0780037551.01.T01 MTR_7g007080 34.667 300 152 13 27 307 103 377 4.68e-34 128
MsG0780037551.01.T01 MTR_2g020180 48.529 136 55 4 177 308 90 214 6.86e-34 124
MsG0780037551.01.T01 MTR_3g115430 51.181 127 54 3 187 308 1 124 1.44e-33 120
MsG0780037551.01.T01 MTR_1g108800 37.743 257 136 9 66 307 93 340 4.92e-33 124
MsG0780037551.01.T01 MTR_5g073420 32.278 316 185 13 4 308 82 379 6.74e-33 125
MsG0780037551.01.T01 MTR_2g007450 31.329 316 170 11 27 307 115 418 7.39e-33 125
MsG0780037551.01.T01 MTR_1g108820 35.294 272 141 10 62 307 122 384 7.07e-32 122
MsG0780037551.01.T01 MTR_4g080610 35.827 254 138 11 76 309 109 357 7.26e-32 122
MsG0780037551.01.T01 MTR_1g085120 31.250 320 184 11 9 308 167 470 4.31e-31 121
MsG0780037551.01.T01 MTR_5g063950 32.492 317 179 12 8 306 60 359 4.43e-31 120
MsG0780037551.01.T01 MTR_1g085120 31.250 320 184 11 9 308 242 545 7.54e-31 122
MsG0780037551.01.T01 MTR_5g058000 33.871 310 177 10 9 308 83 374 1.07e-30 119
MsG0780037551.01.T01 MTR_5g039470 32.877 292 155 12 4 273 55 327 1.27e-30 118
MsG0780037551.01.T01 MTR_2g007400 31.068 309 170 12 26 307 109 401 2.23e-30 119
MsG0780037551.01.T01 MTR_6g011460 33.437 323 179 12 4 308 116 420 5.53e-30 118
MsG0780037551.01.T01 MTR_2g005990 33.117 308 169 12 23 307 100 393 2.50e-29 115
MsG0780037551.01.T01 MTR_3g108560 35.931 231 124 8 1 221 83 299 5.33e-29 113
MsG0780037551.01.T01 MTR_7g060470 31.379 290 156 11 14 277 87 359 1.50e-28 113
MsG0780037551.01.T01 MTR_5g073370 33.333 294 169 12 10 287 77 359 1.97e-28 113
MsG0780037551.01.T01 MTR_7g114710 31.949 313 176 10 4 308 80 363 2.72e-28 112
MsG0780037551.01.T01 MTR_5g045510 30.938 320 174 12 4 307 82 370 3.71e-28 112
MsG0780037551.01.T01 MTR_1g053025 28.990 307 170 12 27 306 101 386 5.68e-28 112
MsG0780037551.01.T01 MTR_3g107120 32.075 318 173 13 10 306 93 388 7.48e-28 112
MsG0780037551.01.T01 MTR_5g031970 33.227 313 168 13 8 309 74 356 1.45e-27 110
MsG0780037551.01.T01 MTR_0009s0080 32.542 295 151 12 10 277 94 367 3.60e-27 110
MsG0780037551.01.T01 MTR_6g040270 33.721 258 147 11 66 307 130 379 7.25e-27 108
MsG0780037551.01.T01 MTR_6g022520 30.581 327 193 11 1 308 1 312 8.66e-27 107
MsG0780037551.01.T01 MTR_5g054920 34.171 199 108 8 107 291 2 191 8.93e-27 104
MsG0780037551.01.T01 MTR_2g036470 30.275 327 179 14 11 309 91 396 1.12e-26 108
MsG0780037551.01.T01 MTR_5g039450 32.636 239 127 9 88 306 131 355 1.42e-26 107
MsG0780037551.01.T01 MTR_4g039440 30.211 331 194 13 1 308 95 411 1.78e-26 108
MsG0780037551.01.T01 MTR_3g107160 30.508 295 174 12 26 306 103 380 5.79e-26 106
MsG0780037551.01.T01 MTR_1g107385 31.203 266 146 10 66 307 169 421 1.28e-25 105
MsG0780037551.01.T01 MTR_3g107940 44.000 125 58 3 181 301 89 205 9.17e-25 102
MsG0780037551.01.T01 MTR_4g098590 35.424 271 144 12 27 277 95 354 1.21e-24 102
MsG0780037551.01.T01 MTR_3g099830 29.836 305 176 10 30 307 79 372 1.34e-24 102
MsG0780037551.01.T01 MTR_7g021990 34.646 254 139 10 67 299 168 415 2.82e-24 102
MsG0780037551.01.T01 MTR_7g075820 30.798 263 148 10 65 307 144 392 4.43e-24 101
MsG0780037551.01.T01 MTR_7g021950 34.646 254 139 10 67 299 168 415 4.61e-24 102
MsG0780037551.01.T01 MTR_7g055943 30.462 325 182 13 9 308 115 420 6.05e-24 101
MsG0780037551.01.T01 MTR_7g022105 33.333 249 141 8 67 293 169 414 8.43e-24 100
MsG0780037551.01.T01 MTR_2g007360 31.655 278 126 15 73 307 116 372 3.08e-23 98.6
MsG0780037551.01.T01 MTR_5g039490 29.503 322 164 11 8 307 94 374 5.43e-23 98.2
MsG0780037551.01.T01 MTR_1089s0010 30.650 323 183 14 6 308 77 378 7.61e-23 97.8
MsG0780037551.01.T01 MTR_6g022360 31.538 260 158 9 66 309 153 408 9.36e-23 97.8
MsG0780037551.01.T01 MTR_7g083740 32.340 235 131 9 92 309 144 367 1.20e-22 97.1
MsG0780037551.01.T01 MTR_3g026920 32.090 268 123 12 66 306 167 402 3.09e-22 96.3
MsG0780037551.01.T01 MTR_2g007500 33.333 258 130 11 27 256 91 334 3.51e-22 95.1
MsG0780037551.01.T01 MTR_1g043170 51.163 86 38 2 224 308 5 87 6.45e-22 88.2
MsG0780037551.01.T01 MTR_2g007090 31.034 232 125 10 88 308 86 293 9.53e-22 93.2
MsG0780037551.01.T01 MTR_7g007340 28.105 306 184 11 20 303 60 351 9.74e-22 94.4
MsG0780037551.01.T01 MTR_2g007550 33.010 206 92 9 130 306 144 332 1.74e-21 93.6
MsG0780037551.01.T01 MTR_5g083890 30.502 259 144 10 65 298 144 391 1.85e-21 94.7
MsG0780037551.01.T01 MTR_2g008310 28.889 270 155 12 60 307 115 369 2.13e-21 94.0
MsG0780037551.01.T01 MTR_6g029350 32.536 209 115 10 111 307 2 196 4.42e-21 90.5
MsG0780037551.01.T01 MTR_7g034090 28.395 324 129 14 1 308 75 311 1.20e-20 92.0
MsG0780037551.01.T01 MTR_3g110010 35.122 205 106 9 65 246 222 422 9.43e-20 90.1
MsG0780037551.01.T01 MTR_3g110010 31.111 225 125 9 1 215 76 280 1.47e-13 71.6
MsG0780037551.01.T01 MTR_3g113770 32.850 207 81 6 67 267 180 334 1.08e-19 88.2
MsG0780037551.01.T01 MTR_2g008320 28.777 278 147 13 60 307 169 425 3.13e-19 87.8
MsG0780037551.01.T01 MTR_5g065100 31.615 291 149 11 28 309 100 349 3.37e-19 87.8
MsG0780037551.01.T01 MTR_1g059860 28.571 315 156 12 8 270 14 311 4.91e-19 86.3
MsG0780037551.01.T01 MTR_5g008910 44.944 89 45 2 221 308 216 301 5.11e-19 85.9
MsG0780037551.01.T01 MTR_8g077460 28.479 309 188 12 10 308 89 374 1.11e-18 85.9
MsG0780037551.01.T01 MTR_3g095630 32.941 170 101 6 137 299 12 175 1.39e-18 82.4
MsG0780037551.01.T01 MTR_2g007610 35.714 140 75 3 173 307 20 149 5.90e-18 80.1
MsG0780037551.01.T01 MTR_3g113785 44.211 95 39 2 181 267 4 92 6.71e-18 77.8
MsG0780037551.01.T01 MTR_5g024060 33.613 119 77 1 181 299 82 198 6.80e-18 82.0
MsG0780037551.01.T01 MTR_3g108140 48.148 81 41 1 230 309 68 148 2.51e-17 78.6
MsG0780037551.01.T01 MTR_6g044572 27.687 307 166 12 21 308 118 387 6.45e-17 80.9
MsG0780037551.01.T01 MTR_8g070950 27.731 357 184 14 10 308 93 433 7.04e-17 80.9
MsG0780037551.01.T01 MTR_5g073340 32.057 209 113 7 60 246 99 300 1.33e-16 79.3
MsG0780037551.01.T01 MTR_6g044560 28.339 307 164 13 21 308 294 563 2.75e-16 79.7
MsG0780037551.01.T01 MTR_3g071790 29.444 180 113 6 105 271 24 202 7.12e-16 75.1
MsG0780037551.01.T01 MTR_4g134150 33.523 176 95 7 1 172 73 230 8.27e-16 75.5
MsG0780037551.01.T01 MTR_3g106960 40.000 125 63 5 181 301 74 190 9.58e-16 75.5
MsG0780037551.01.T01 MTR_4g096730 46.250 80 39 2 230 308 12 88 1.74e-15 71.2
MsG0780037551.01.T01 MTR_7g033940 58.462 65 25 1 1 65 157 219 1.85e-15 74.3
MsG0780037551.01.T01 MTR_7g007370 31.538 260 137 10 65 305 96 333 4.29e-15 75.1
MsG0780037551.01.T01 MTR_1377s0010 32.143 140 83 4 153 292 10 137 4.87e-15 71.2
MsG0780037551.01.T01 MTR_7g056280 38.938 113 56 4 175 277 437 546 3.94e-14 73.2
MsG0780037551.01.T01 MTR_1g108820 33.333 183 93 8 62 220 122 299 4.00e-14 72.4
MsG0780037551.01.T01 MTR_3g094980 31.579 114 71 2 169 277 6 117 1.12e-13 68.6
MsG0780037551.01.T01 MTR_5g033200 32.335 167 98 7 141 299 13 172 1.44e-13 68.2
MsG0780037551.01.T01 MTR_5g076150 35.096 208 97 10 67 242 26 227 1.46e-13 69.3
MsG0780037551.01.T01 MTR_8g014210 34.513 113 73 1 196 308 119 230 1.67e-13 69.3
MsG0780037551.01.T01 MTR_5g092960 36.842 114 65 2 200 308 350 461 2.60e-13 70.5
MsG0780037551.01.T01 MTR_7g056447 41.129 124 58 5 1 119 72 185 2.07e-12 67.0
MsG0780037551.01.T01 MTR_5g084070 30.970 268 125 8 67 277 130 394 2.45e-12 67.4
MsG0780037551.01.T01 MTR_8g058970 33.036 112 71 2 201 308 365 476 3.06e-12 67.4
MsG0780037551.01.T01 MTR_7g056417 37.333 150 76 7 66 198 10 158 3.08e-12 63.9
MsG0780037551.01.T01 MTR_8g058965 25.770 357 177 17 28 308 111 455 4.71e-12 66.6
MsG0780037551.01.T01 MTR_7g075800 25.342 292 155 14 36 273 109 391 8.31e-12 66.2
MsG0780037551.01.T01 MTR_8g467800 31.429 140 83 4 177 308 318 452 1.35e-11 65.1
MsG0780037551.01.T01 MTR_5g061680 29.150 247 134 12 4 244 82 293 2.06e-11 64.3
MsG0780037551.01.T01 MTR_7g022060 32.432 111 69 2 199 303 214 324 2.98e-11 63.5
MsG0780037551.01.T01 MTR_8g062310 24.507 355 189 13 5 308 101 427 5.29e-11 63.5
MsG0780037551.01.T01 MTR_8g469020 35.878 131 77 4 181 308 324 450 6.98e-11 63.2
MsG0780037551.01.T01 MTR_8g062010 34.586 133 76 4 181 308 303 429 8.00e-11 62.8
MsG0780037551.01.T01 MTR_5g080710 31.579 133 85 3 180 309 325 454 8.97e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037551.01.T01 AT5G56370 29.444 360 180 14 10 308 74 420 1.04e-23 100
MsG0780037551.01.T01 AT5G56370 29.444 360 180 14 10 308 74 420 1.04e-23 100
MsG0780037551.01.T01 AT1G32375 25.212 353 205 11 10 308 74 421 5.05e-21 93.2
MsG0780037551.01.T01 AT1G32375 25.212 353 205 11 10 308 74 421 5.05e-21 93.2
MsG0780037551.01.T01 AT4G26340 27.326 344 182 13 23 308 85 418 1.93e-17 82.8
MsG0780037551.01.T01 AT3G52680 28.689 366 180 15 10 308 93 444 4.38e-17 81.6
MsG0780037551.01.T01 AT4G10400 28.571 357 188 14 2 308 69 408 5.53e-17 81.3
MsG0780037551.01.T01 AT4G10400 28.571 357 188 14 2 308 69 408 5.53e-17 81.3
MsG0780037551.01.T01 AT4G10400 28.571 357 188 14 2 308 69 408 5.53e-17 81.3
MsG0780037551.01.T01 AT4G00160 28.453 362 187 16 10 308 89 441 3.95e-16 79.0
MsG0780037551.01.T01 AT5G56380 24.202 376 206 12 1 308 74 438 5.88e-16 78.2
MsG0780037551.01.T01 AT5G56380 24.202 376 206 12 1 308 74 438 5.88e-16 78.2
MsG0780037551.01.T01 AT3G54910 28.212 358 192 14 2 308 29 372 3.30e-15 75.9
MsG0780037551.01.T01 AT3G54910 28.212 358 192 14 2 308 29 372 3.30e-15 75.9
MsG0780037551.01.T01 AT3G54910 28.212 358 192 14 2 308 29 372 3.30e-15 75.9
MsG0780037551.01.T01 AT5G56800 32.143 196 107 10 129 308 158 343 6.69e-13 68.9
MsG0780037551.01.T01 AT3G49030 31.868 182 109 7 139 308 262 440 1.02e-12 68.6
MsG0780037551.01.T01 AT5G38580 29.143 175 104 4 140 309 5 164 1.05e-12 65.5
MsG0780037551.01.T01 AT5G44940 27.915 283 172 13 10 278 81 345 3.07e-12 67.0
MsG0780037551.01.T01 AT1G50980 27.303 304 187 10 7 297 76 358 1.37e-11 65.1
MsG0780037551.01.T01 AT5G56390 25.000 296 156 12 73 308 135 424 3.54e-11 63.9
MsG0780037551.01.T01 AT5G38565 36.207 116 69 2 195 307 226 339 4.05e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAGGACCTAATTTGTTTA+AGG 0.146058 7:-28259508 None:intergenic
CTCCAGTGGATTTAACTTTA+AGG 0.151985 7:+28260349 MsG0780037551.01.T01:CDS
AGTTGAGAGGAAAGACATTT+TGG 0.242529 7:-28260219 None:intergenic
TTACTAACCTTAAACAAATT+AGG 0.294724 7:+28259501 MsG0780037551.01.T01:CDS
AATGTTTGCTCTGACCAAAT+TGG 0.301668 7:-28259766 None:intergenic
GTTTAAACACTTGCCCAATT+TGG 0.316334 7:+28259752 MsG0780037551.01.T01:CDS
ATCCTGGATTTGAGTAAAAC+TGG 0.350921 7:+28259471 MsG0780037551.01.T01:CDS
TTTAAGGCACCAAATGCTAA+TGG 0.357736 7:+28260365 MsG0780037551.01.T01:CDS
CCTACTCAAAACTCTCCATT+TGG 0.373266 7:+28259617 MsG0780037551.01.T01:CDS
ATGTTTGCTCTGACCAAATT+GGG 0.383897 7:-28259765 None:intergenic
GTTTGGTAGGGAGAAAAGAT+AGG 0.389170 7:-28259194 None:intergenic
CTATGTATGCATTTGAAAGA+TGG 0.393659 7:+28259720 MsG0780037551.01.T01:CDS
CCTCAAATGTGGTGCCTCTA+GGG 0.396461 7:+28259383 MsG0780037551.01.T01:CDS
AGGATAAATCCATTAGCATT+TGG 0.400015 7:-28260374 None:intergenic
CACGAAATATTCAATTTGCT+TGG 0.403798 7:+28259802 MsG0780037551.01.T01:CDS
ATCCACTGGAGAGGCACTAA+TGG 0.429851 7:-28260337 None:intergenic
ATGGTATTGACGAGGATGAT+TGG 0.432079 7:+28260178 MsG0780037551.01.T01:CDS
TATTTCCAACTCCTCTAGAA+TGG 0.432132 7:-28259688 None:intergenic
GCTTGTTGCGGCAGATTGTT+TGG 0.436931 7:-28259211 None:intergenic
AAGGGGCAAGGTGGGGTCAC+GGG 0.441252 7:-28259349 None:intergenic
ATGTGGCAGATAACGGAATC+AGG 0.445476 7:-28259171 None:intergenic
GCCTCAAATGTGGTGCCTCT+AGG 0.451267 7:+28259382 MsG0780037551.01.T01:CDS
CAAAACTCTCCATTTGGAAA+GGG 0.451508 7:+28259623 MsG0780037551.01.T01:CDS
TGCCTTAAAGTTAAATCCAC+TGG 0.452060 7:-28260351 None:intergenic
GTTGCGGCAGATTGTTTGGT+AGG 0.455605 7:-28259207 None:intergenic
CAAGCATCCTCTCTAAAAGT+TGG 0.459176 7:+28259229 MsG0780037551.01.T01:five_prime_UTR
GAATCGCAACCCTTTCCAAA+TGG 0.459428 7:-28259632 None:intergenic
CCCTAGAGGCACCACATTTG+AGG 0.460792 7:-28259383 None:intergenic
AGGAACTCAGAATTTAATCC+TGG 0.462268 7:+28259455 MsG0780037551.01.T01:CDS
GAAAGATTGAAGGGGCAAGG+TGG 0.466053 7:-28259358 None:intergenic
TTTCTAGCAACCACTTCCAT+TGG 0.467611 7:-28260018 None:intergenic
ATGCATTTGAAAGATGGTAT+TGG 0.467638 7:+28259726 MsG0780037551.01.T01:CDS
TGGATTTATCCTTGTGTCCA+AGG 0.470063 7:+28260385 MsG0780037551.01.T01:CDS
AGCTTATATTCACATCAAAC+TGG 0.472459 7:+28259990 MsG0780037551.01.T01:CDS
ATTTCCAACTCCTCTAGAAT+GGG 0.472558 7:-28259687 None:intergenic
GGATTTATCCTTGTGTCCAA+GGG 0.479981 7:+28260386 MsG0780037551.01.T01:CDS
GAAGGGGCAAGGTGGGGTCA+CGG 0.482084 7:-28259350 None:intergenic
TTCTAGCAACCACTTCCATT+GGG 0.485071 7:-28260017 None:intergenic
AGATAGGATGTGGCAGATAA+CGG 0.499752 7:-28259178 None:intergenic
GGTCATTGTGCGCAATAAGT+TGG 0.500692 7:-28260316 None:intergenic
AAAGATTGAAGGGGCAAGGT+GGG 0.501450 7:-28259357 None:intergenic
ATTTGAGGCGGAAAGATTGA+AGG 0.501477 7:-28259368 None:intergenic
CCTCTAGGGGATGCGACCCA+CGG 0.507168 7:+28259397 MsG0780037551.01.T01:CDS
AGTAGGGGAAAATCGATTTG+AGG 0.510436 7:-28259600 None:intergenic
TTGTCCCATTCTAGAGGAGT+TGG 0.515673 7:+28259683 MsG0780037551.01.T01:CDS
CTCTAGGGGATGCGACCCAC+GGG 0.516654 7:+28259398 MsG0780037551.01.T01:CDS
TCAAAACTCTCCATTTGGAA+AGG 0.519588 7:+28259622 MsG0780037551.01.T01:CDS
TTGCGGCAGATTGTTTGGTA+GGG 0.542710 7:-28259206 None:intergenic
CAATCTTTCCGCCTCAAATG+TGG 0.543733 7:+28259372 MsG0780037551.01.T01:CDS
TAGGGAGAAAAGATAGGATG+TGG 0.550081 7:-28259188 None:intergenic
TTGAAAATTGCGGAAACTGT+AGG 0.567303 7:-28259525 None:intergenic
AGTAGAGCCCCTTGGACACA+AGG 0.567704 7:-28260394 None:intergenic
CATAAATACAACCTTGTGAA+GGG 0.570727 7:-28260074 None:intergenic
CGAGCATGTTGTTAGTTGAG+AGG 0.570954 7:-28260232 None:intergenic
CTCAAATGTGGTGCCTCTAG+GGG 0.573015 7:+28259384 MsG0780037551.01.T01:CDS
ACATAAATACAACCTTGTGA+AGG 0.576752 7:-28260075 None:intergenic
TTTCAATTCTAGAAACCTTG+TGG 0.578779 7:+28259542 MsG0780037551.01.T01:CDS
ACTGGCGTACCCAATGGAAG+TGG 0.580901 7:+28260008 MsG0780037551.01.T01:CDS
CATCAAACTGGCGTACCCAA+TGG 0.582757 7:+28260002 MsG0780037551.01.T01:CDS
TTACATGCAGTAGAGCCCCT+TGG 0.584475 7:-28260402 None:intergenic
TGACATAATAAACAAAATGT+CGG 0.585470 7:-28259320 None:intergenic
GTTTCTAGAATTGAAAATTG+CGG 0.585473 7:-28259535 None:intergenic
TTTGAGGCGGAAAGATTGAA+GGG 0.589352 7:-28259367 None:intergenic
CGAGGGTTGTCCCATTCTAG+AGG 0.590341 7:+28259677 MsG0780037551.01.T01:CDS
ACAAATCGATTGATATCCCG+TGG 0.592319 7:-28259414 None:intergenic
GTGAGGAGTGACAGCCACAA+TGG 0.593003 7:-28259255 None:intergenic
GACCATTAGTGCCTCTCCAG+TGG 0.593235 7:+28260335 MsG0780037551.01.T01:CDS
GCGGAAAGATTGAAGGGGCA+AGG 0.605442 7:-28259361 None:intergenic
CTAAAAGTTGGAATCCATTG+TGG 0.605471 7:+28259241 MsG0780037551.01.T01:five_prime_UTR
TAGCGAAATCTGCAAAGTCA+TGG 0.610175 7:-28259293 None:intergenic
TTTAGAGAGGATGCTTGTTG+CGG 0.611665 7:-28259223 None:intergenic
TCAAAATCTTACCCTTCACA+AGG 0.620543 7:+28260063 MsG0780037551.01.T01:CDS
CCGTGGGTCGCATCCCCTAG+AGG 0.621604 7:-28259397 None:intergenic
AAGATTGAAGGGGCAAGGTG+GGG 0.631819 7:-28259356 None:intergenic
GGTCGTCGAAGTCGAGAGTG+AGG 0.632737 7:-28259272 None:intergenic
CTGGATTTGAGTAAAACTGG+TGG 0.651993 7:+28259474 MsG0780037551.01.T01:CDS
TAAAGTTAAATCCACTGGAG+AGG 0.656760 7:-28260346 None:intergenic
TTGAGGCGGAAAGATTGAAG+GGG 0.666563 7:-28259366 None:intergenic
GATTTATCCTTGTGTCCAAG+GGG 0.668532 7:+28260387 MsG0780037551.01.T01:CDS
TAGAGGCACCACATTTGAGG+CGG 0.672380 7:-28259380 None:intergenic
TGCTCGCTTATAGATTACAA+AGG 0.689162 7:+28260249 MsG0780037551.01.T01:CDS
TTATGAACATGGTATTGACG+AGG 0.690070 7:+28260170 MsG0780037551.01.T01:CDS
TATTCAACTTAAGAACCACA+AGG 0.691826 7:-28259557 None:intergenic
CAAATCGATTGATATCCCGT+GGG 0.691911 7:-28259413 None:intergenic
GTTCGCGCTGCTGTGCAACG+AGG 0.699890 7:+28259435 MsG0780037551.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTCTTTTTATG+TGG + Chr7:28260135-28260154 MsG0780037551.01.T01:intron 10.0%
!! TGACATAATAAACAAAATGT+CGG - Chr7:28259323-28259342 None:intergenic 20.0%
!! TTACTAACCTTAAACAAATT+AGG + Chr7:28259501-28259520 MsG0780037551.01.T01:CDS 20.0%
!! TTTTCATAACTTAACTCACA+TGG + Chr7:28259967-28259986 MsG0780037551.01.T01:CDS 25.0%
!!! AGACATTTTGGAATAATTTG+TGG - Chr7:28260210-28260229 None:intergenic 25.0%
!!! GTTTCTAGAATTGAAAATTG+CGG - Chr7:28259538-28259557 None:intergenic 25.0%
ACATAAATACAACCTTGTGA+AGG - Chr7:28260078-28260097 None:intergenic 30.0%
AGCTTATATTCACATCAAAC+TGG + Chr7:28259990-28260009 MsG0780037551.01.T01:CDS 30.0%
AGGATAAATCCATTAGCATT+TGG - Chr7:28260377-28260396 None:intergenic 30.0%
ATGCATTTGAAAGATGGTAT+TGG + Chr7:28259726-28259745 MsG0780037551.01.T01:CDS 30.0%
CATAAATACAACCTTGTGAA+GGG - Chr7:28260077-28260096 None:intergenic 30.0%
CTATGTATGCATTTGAAAGA+TGG + Chr7:28259720-28259739 MsG0780037551.01.T01:CDS 30.0%
TATTCAACTTAAGAACCACA+AGG - Chr7:28259560-28259579 None:intergenic 30.0%
! AAAGTTTTTGCGTTTAGAAG+TGG + Chr7:28259836-28259855 MsG0780037551.01.T01:CDS 30.0%
! CACGAAATATTCAATTTGCT+TGG + Chr7:28259802-28259821 MsG0780037551.01.T01:CDS 30.0%
! TTTCAATTCTAGAAACCTTG+TGG + Chr7:28259542-28259561 MsG0780037551.01.T01:CDS 30.0%
!! AGATTTTGATAAGCTTATCG+AGG + Chr7:28259659-28259678 MsG0780037551.01.T01:CDS 30.0%
!! GATTTTGATAAGCTTATCGA+GGG + Chr7:28259660-28259679 MsG0780037551.01.T01:CDS 30.0%
!! TGTAGGACCTAATTTGTTTA+AGG - Chr7:28259511-28259530 None:intergenic 30.0%
!! TTTTGACAGCTTTATGAACA+TGG + Chr7:28260159-28260178 MsG0780037551.01.T01:intron 30.0%
!!! AGGGTAAGATTTTGAAGTAT+AGG - Chr7:28260058-28260077 None:intergenic 30.0%
!!! GGGTAAGATTTTGAAGTATA+GGG - Chr7:28260057-28260076 None:intergenic 30.0%
AATGTTTGCTCTGACCAAAT+TGG - Chr7:28259769-28259788 None:intergenic 35.0%
AGGAACTCAGAATTTAATCC+TGG + Chr7:28259455-28259474 MsG0780037551.01.T01:CDS 35.0%
ATCCTGGATTTGAGTAAAAC+TGG + Chr7:28259471-28259490 MsG0780037551.01.T01:CDS 35.0%
ATGTTTGCTCTGACCAAATT+GGG - Chr7:28259768-28259787 None:intergenic 35.0%
CAAAACTCTCCATTTGGAAA+GGG + Chr7:28259623-28259642 MsG0780037551.01.T01:CDS 35.0%
CTAAAAGTTGGAATCCATTG+TGG + Chr7:28259241-28259260 MsG0780037551.01.T01:five_prime_UTR 35.0%
GTTTAAACACTTGCCCAATT+TGG + Chr7:28259752-28259771 MsG0780037551.01.T01:CDS 35.0%
TAAAGTTAAATCCACTGGAG+AGG - Chr7:28260349-28260368 None:intergenic 35.0%
TCAAAACTCTCCATTTGGAA+AGG + Chr7:28259622-28259641 MsG0780037551.01.T01:CDS 35.0%
TCAAAATCTTACCCTTCACA+AGG + Chr7:28260063-28260082 MsG0780037551.01.T01:CDS 35.0%
TGCCTTAAAGTTAAATCCAC+TGG - Chr7:28260354-28260373 None:intergenic 35.0%
TGCTCGCTTATAGATTACAA+AGG + Chr7:28260249-28260268 MsG0780037551.01.T01:CDS 35.0%
TTGAAAATTGCGGAAACTGT+AGG - Chr7:28259528-28259547 None:intergenic 35.0%
! AGTTGAGAGGAAAGACATTT+TGG - Chr7:28260222-28260241 None:intergenic 35.0%
! CTCCAGTGGATTTAACTTTA+AGG + Chr7:28260349-28260368 MsG0780037551.01.T01:CDS 35.0%
! TATTTCCAACTCCTCTAGAA+TGG - Chr7:28259691-28259710 None:intergenic 35.0%
! TTTAAGGCACCAAATGCTAA+TGG + Chr7:28260365-28260384 MsG0780037551.01.T01:CDS 35.0%
!! ATGGATTCCAACTTTTAGAG+AGG - Chr7:28259239-28259258 None:intergenic 35.0%
!! ATTTCCAACTCCTCTAGAAT+GGG - Chr7:28259690-28259709 None:intergenic 35.0%
!! TTATGAACATGGTATTGACG+AGG + Chr7:28260170-28260189 MsG0780037551.01.T01:CDS 35.0%
!!! AAATGGAGAGTTTTGAGTAG+GGG - Chr7:28259618-28259637 None:intergenic 35.0%
!!! CAAATGGAGAGTTTTGAGTA+GGG - Chr7:28259619-28259638 None:intergenic 35.0%
ACAAATCGATTGATATCCCG+TGG - Chr7:28259417-28259436 None:intergenic 40.0%
AGATAGGATGTGGCAGATAA+CGG - Chr7:28259181-28259200 None:intergenic 40.0%
CAAATCGATTGATATCCCGT+GGG - Chr7:28259416-28259435 None:intergenic 40.0%
CCTACTCAAAACTCTCCATT+TGG + Chr7:28259617-28259636 MsG0780037551.01.T01:CDS 40.0%
CTGGATTTGAGTAAAACTGG+TGG + Chr7:28259474-28259493 MsG0780037551.01.T01:CDS 40.0%
GATTTATCCTTGTGTCCAAG+GGG + Chr7:28260387-28260406 MsG0780037551.01.T01:CDS 40.0%
GGATTTATCCTTGTGTCCAA+GGG + Chr7:28260386-28260405 MsG0780037551.01.T01:CDS 40.0%
GTTTGGTAGGGAGAAAAGAT+AGG - Chr7:28259197-28259216 None:intergenic 40.0%
TAGCGAAATCTGCAAAGTCA+TGG - Chr7:28259296-28259315 None:intergenic 40.0%
TAGGGAGAAAAGATAGGATG+TGG - Chr7:28259191-28259210 None:intergenic 40.0%
TGGATTTATCCTTGTGTCCA+AGG + Chr7:28260385-28260404 MsG0780037551.01.T01:CDS 40.0%
TTCTAGCAACCACTTCCATT+GGG - Chr7:28260020-28260039 None:intergenic 40.0%
! ATTTGAGGCGGAAAGATTGA+AGG - Chr7:28259371-28259390 None:intergenic 40.0%
! CAAGCATCCTCTCTAAAAGT+TGG + Chr7:28259229-28259248 MsG0780037551.01.T01:five_prime_UTR 40.0%
! CACCAGTTTTACTCAAATCC+AGG - Chr7:28259476-28259495 None:intergenic 40.0%
! TTTAGAGAGGATGCTTGTTG+CGG - Chr7:28259226-28259245 None:intergenic 40.0%
! TTTCTAGCAACCACTTCCAT+TGG - Chr7:28260021-28260040 None:intergenic 40.0%
! TTTGAGGCGGAAAGATTGAA+GGG - Chr7:28259370-28259389 None:intergenic 40.0%
!! AGTAGGGGAAAATCGATTTG+AGG - Chr7:28259603-28259622 None:intergenic 40.0%
!! ATGGTATTGACGAGGATGAT+TGG + Chr7:28260178-28260197 MsG0780037551.01.T01:CDS 40.0%
!!! CCAAATGGAGAGTTTTGAGT+AGG - Chr7:28259620-28259639 None:intergenic 40.0%
AAAGATTGAAGGGGCAAGGT+GGG - Chr7:28259360-28259379 None:intergenic 45.0%
CAATCTTTCCGCCTCAAATG+TGG + Chr7:28259372-28259391 MsG0780037551.01.T01:CDS 45.0%
CGAGCATGTTGTTAGTTGAG+AGG - Chr7:28260235-28260254 None:intergenic 45.0%
GAATCGCAACCCTTTCCAAA+TGG - Chr7:28259635-28259654 None:intergenic 45.0%
GGTCATTGTGCGCAATAAGT+TGG - Chr7:28260319-28260338 None:intergenic 45.0%
TTGAGGCGGAAAGATTGAAG+GGG - Chr7:28259369-28259388 None:intergenic 45.0%
TTGCGGCAGATTGTTTGGTA+GGG - Chr7:28259209-28259228 None:intergenic 45.0%
! TTGTCCCATTCTAGAGGAGT+TGG + Chr7:28259683-28259702 MsG0780037551.01.T01:CDS 45.0%
AAGATTGAAGGGGCAAGGTG+GGG - Chr7:28259359-28259378 None:intergenic 50.0%
CATCAAACTGGCGTACCCAA+TGG + Chr7:28260002-28260021 MsG0780037551.01.T01:CDS 50.0%
CCTCAAATGTGGTGCCTCTA+GGG + Chr7:28259383-28259402 MsG0780037551.01.T01:CDS 50.0%
CTCAAATGTGGTGCCTCTAG+GGG + Chr7:28259384-28259403 MsG0780037551.01.T01:CDS 50.0%
GAAAGATTGAAGGGGCAAGG+TGG - Chr7:28259361-28259380 None:intergenic 50.0%
GTTGCGGCAGATTGTTTGGT+AGG - Chr7:28259210-28259229 None:intergenic 50.0%
TTACATGCAGTAGAGCCCCT+TGG - Chr7:28260405-28260424 None:intergenic 50.0%
! GCTTGTTGCGGCAGATTGTT+TGG - Chr7:28259214-28259233 None:intergenic 50.0%
!! ATCCACTGGAGAGGCACTAA+TGG - Chr7:28260340-28260359 None:intergenic 50.0%
!! TAGAGGCACCACATTTGAGG+CGG - Chr7:28259383-28259402 None:intergenic 50.0%
ACTGGCGTACCCAATGGAAG+TGG + Chr7:28260008-28260027 MsG0780037551.01.T01:CDS 55.0%
AGTAGAGCCCCTTGGACACA+AGG - Chr7:28260397-28260416 None:intergenic 55.0%
ATGCTCGCTACGCTGTTGCA+TGG - Chr7:28259936-28259955 None:intergenic 55.0%
CGAGGGTTGTCCCATTCTAG+AGG + Chr7:28259677-28259696 MsG0780037551.01.T01:CDS 55.0%
GACCATTAGTGCCTCTCCAG+TGG + Chr7:28260335-28260354 MsG0780037551.01.T01:CDS 55.0%
GCCTCAAATGTGGTGCCTCT+AGG + Chr7:28259382-28259401 MsG0780037551.01.T01:CDS 55.0%
GCGGAAAGATTGAAGGGGCA+AGG - Chr7:28259364-28259383 None:intergenic 55.0%
GTGAGGAGTGACAGCCACAA+TGG - Chr7:28259258-28259277 None:intergenic 55.0%
TGCTCGCTACGCTGTTGCAT+GGG - Chr7:28259935-28259954 None:intergenic 55.0%
!! CCCTAGAGGCACCACATTTG+AGG - Chr7:28259386-28259405 None:intergenic 55.0%
GGTCGTCGAAGTCGAGAGTG+AGG - Chr7:28259275-28259294 None:intergenic 60.0%
AAGGGGCAAGGTGGGGTCAC+GGG - Chr7:28259352-28259371 None:intergenic 65.0%
GAAGGGGCAAGGTGGGGTCA+CGG - Chr7:28259353-28259372 None:intergenic 65.0%
GTTCGCGCTGCTGTGCAACG+AGG + Chr7:28259435-28259454 MsG0780037551.01.T01:CDS 65.0%
! CCTCTAGGGGATGCGACCCA+CGG + Chr7:28259397-28259416 MsG0780037551.01.T01:CDS 65.0%
! CTCTAGGGGATGCGACCCAC+GGG + Chr7:28259398-28259417 MsG0780037551.01.T01:CDS 65.0%
CCGTGGGTCGCATCCCCTAG+AGG - Chr7:28259400-28259419 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 28259172 28260440 28259172 ID=MsG0780037551.01;Name=MsG0780037551.01
Chr7 mRNA 28259172 28260440 28259172 ID=MsG0780037551.01.T01;Parent=MsG0780037551.01;Name=MsG0780037551.01.T01;_AED=0.36;_eAED=0.36;_QI=170|0.5|0.33|1|1|1|3|0|309
Chr7 exon 28259172 28259857 28259172 ID=MsG0780037551.01.T01:exon:13096;Parent=MsG0780037551.01.T01
Chr7 exon 28259944 28260084 28259944 ID=MsG0780037551.01.T01:exon:13097;Parent=MsG0780037551.01.T01
Chr7 exon 28260168 28260440 28260168 ID=MsG0780037551.01.T01:exon:13098;Parent=MsG0780037551.01.T01
Chr7 five_prime_UTR 28259172 28259341 28259172 ID=MsG0780037551.01.T01:five_prime_utr;Parent=MsG0780037551.01.T01
Chr7 CDS 28259342 28259857 28259342 ID=MsG0780037551.01.T01:cds;Parent=MsG0780037551.01.T01
Chr7 CDS 28259944 28260084 28259944 ID=MsG0780037551.01.T01:cds;Parent=MsG0780037551.01.T01
Chr7 CDS 28260168 28260440 28260168 ID=MsG0780037551.01.T01:cds;Parent=MsG0780037551.01.T01
Gene Sequence

>MsG0780037551.01.T01

ATGCTCGCCCGTGACCCCACCTTGCCCCTTCAATCTTTCCGCCTCAAATGTGGTGCCTCTAGGGGATGCGACCCACGGGATATCAATCGATTTGTTCGCGCTGCTGTGCAACGAGGAACTCAGAATTTAATCCTGGATTTGAGTAAAACTGGTGGAGCATTACTAACCTTAAACAAATTAGGTCCTACAGTTTCCGCAATTTTCAATTCTAGAAACCTTGTGGTTCTTAAGTTGAATACATTACTAGTGAATGTTCTTCCTCAAATCGATTTTCCCCTACTCAAAACTCTCCATTTGGAAAGGGTTGCGATTCTAAAAGATTTTGATAAGCTTATCGAGGGTTGTCCCATTCTAGAGGAGTTGGAAATAACTTATTTACTATGTATGCATTTGAAAGATGGTATTGGAGAGTTTAAACACTTGCCCAATTTGGTCAGAGCAAACATTTCTGAATTTGTTTCACGAAATATTCAATTTGCTTGGATTTGCAATGCAAAGTTTTTGCGTTTAGAAGTGCGTAGCGAGCATCAAGTTCACACTTTTCATAACTTAACTCACATGGAGCTTATATTCACATCAAACTGGCGTACCCAATGGAAGTGGTTGCTAGAAATGCTGAAGAATTGCCCTATACTTCAAAATCTTACCCTTCACAAGCTTTATGAACATGGTATTGACGAGGATGATTGGAATGAACCACAAATTATTCCAAAATGTCTTTCCTCTCAACTAACAACATGCTCGCTTATAGATTACAAAGGCACGAAGTGTGAGCTTCAGTTTGCAGAGTATGTTTTGAAGAATGCCAACTTATTGCGCACAATGACCATTAGTGCCTCTCCAGTGGATTTAACTTTAAGGCACCAAATGCTAATGGATTTATCCTTGTGTCCAAGGGGCTCTACTGCATGTAAACTTTCATTCATTTGA
Protein sequence

>MsG0780037551.01.T01

MLARDPTLPLQSFRLKCGASRGCDPRDINRFVRAAVQRGTQNLILDLSKTGGALLTLNKLGPTVSAIFNSRNLVVLKLNTLLVNVLPQIDFPLLKTLHLERVAILKDFDKLIEGCPILEELEITYLLCMHLKDGIGEFKHLPNLVRANISEFVSRNIQFAWICNAKFLRLEVRSEHQVHTFHNLTHMELIFTSNWRTQWKWLLEMLKNCPILQNLTLHKLYEHGIDEDDWNEPQIIPKCLSSQLTTCSLIDYKGTKCELQFAEYVLKNANLLRTMTISASPVDLTLRHQMLMDLSLCPRGSTACKLSFI*