AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037556.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037556.01.T01 MTR_7g033915 65.372 309 83 3 1 302 74 365 3.51e-139 397
MsG0780037556.01.T01 MTR_7g033990 67.657 303 88 5 1 294 74 375 3.08e-133 384
MsG0780037556.01.T01 MTR_7g034120 56.869 313 117 6 1 301 1 307 3.84e-115 335
MsG0780037556.01.T01 MTR_1g041245 50.160 313 143 5 1 301 74 385 1.56e-89 272
MsG0780037556.01.T01 MTR_7g034115 48.366 306 142 7 1 294 74 375 7.39e-85 260
MsG0780037556.01.T01 MTR_5g012840 48.387 310 107 6 1 301 52 317 1.55e-80 247
MsG0780037556.01.T01 MTR_7g032380 44.300 307 136 7 3 301 76 355 4.08e-74 231
MsG0780037556.01.T01 MTR_7g032370 42.903 310 153 8 3 301 55 351 2.32e-71 224
MsG0780037556.01.T01 MTR_7g034110 43.003 293 117 5 10 292 80 332 1.89e-61 199
MsG0780037556.01.T01 MTR_5g013500 41.667 324 149 12 1 301 58 364 5.44e-61 198
MsG0780037556.01.T01 MTR_7g098570 43.269 312 156 11 1 297 83 388 9.34e-61 201
MsG0780037556.01.T01 MTR_7g098510 41.693 319 159 14 1 301 81 390 1.54e-60 197
MsG0780037556.01.T01 MTR_0543s0010 40.909 308 164 10 4 302 152 450 7.26e-60 197
MsG0780037556.01.T01 MTR_7g115270 39.144 327 169 11 1 299 75 399 2.37e-59 195
MsG0780037556.01.T01 MTR_7g056433 42.405 316 157 12 5 301 79 388 1.17e-58 192
MsG0780037556.01.T01 MTR_7g088260 40.741 324 153 14 1 301 154 461 2.66e-56 188
MsG0780037556.01.T01 MTR_3g108150 41.693 319 156 13 4 302 79 387 1.90e-54 182
MsG0780037556.01.T01 MTR_0492s0020 40.273 293 157 10 4 287 152 435 8.76e-54 181
MsG0780037556.01.T01 MTR_7g056420 40.385 312 142 10 1 294 72 357 5.21e-51 181
MsG0780037556.01.T01 MTR_7g056430 40.385 312 142 10 1 294 72 357 5.98e-51 181
MsG0780037556.01.T01 MTR_3g087260 42.456 285 133 11 38 302 184 457 3.03e-49 170
MsG0780037556.01.T01 MTR_3g087260 28.646 192 115 4 26 208 84 262 6.45e-12 66.2
MsG0780037556.01.T01 MTR_3g108560 37.580 314 177 9 1 302 83 389 3.93e-49 168
MsG0780037556.01.T01 MTR_5g008950 36.570 309 170 11 10 301 65 364 5.97e-49 167
MsG0780037556.01.T01 MTR_3g082890 37.425 334 169 12 1 301 157 483 5.70e-48 167
MsG0780037556.01.T01 MTR_7g098480 38.019 313 173 12 4 302 84 389 1.90e-47 166
MsG0780037556.01.T01 MTR_3g082890 33.423 371 170 13 1 301 77 440 4.93e-44 155
MsG0780037556.01.T01 MTR_5g008920 36.452 310 173 11 7 301 80 380 1.80e-43 153
MsG0780037556.01.T01 MTR_3g036250 36.164 318 162 13 1 299 68 363 3.54e-43 152
MsG0780037556.01.T01 MTR_7g026500 36.741 313 171 12 9 301 89 394 9.14e-43 151
MsG0780037556.01.T01 MTR_3g109982 39.858 281 138 13 39 294 194 468 2.78e-41 152
MsG0780037556.01.T01 MTR_3g109982 30.455 220 126 9 1 208 76 280 9.25e-14 72.0
MsG0780037556.01.T01 MTR_6g045333 35.127 316 174 12 10 302 139 446 4.36e-40 145
MsG0780037556.01.T01 MTR_5g007410 35.144 313 171 11 10 299 102 405 1.26e-39 144
MsG0780037556.01.T01 MTR_3g023630 33.225 307 186 10 3 299 74 371 2.96e-39 141
MsG0780037556.01.T01 MTR_3g071850 34.700 317 166 13 10 298 102 405 2.55e-37 141
MsG0780037556.01.T01 MTR_7g056287 35.216 301 179 10 9 301 79 371 3.87e-37 136
MsG0780037556.01.T01 MTR_8g085430 35.034 294 122 8 4 290 85 316 9.57e-37 134
MsG0780037556.01.T01 MTR_6g022500 35.065 308 175 11 9 300 176 474 2.45e-36 135
MsG0780037556.01.T01 MTR_7g056277 35.216 301 179 10 9 301 335 627 3.92e-36 137
MsG0780037556.01.T01 MTR_5g045170 34.459 296 163 9 28 302 106 391 7.01e-36 133
MsG0780037556.01.T01 MTR_1g066830 33.231 325 181 13 2 301 73 386 1.04e-35 132
MsG0780037556.01.T01 MTR_5g058000 34.603 315 178 11 2 301 73 374 1.11e-35 132
MsG0780037556.01.T01 MTR_2g020180 41.584 202 95 7 110 301 26 214 1.34e-34 125
MsG0780037556.01.T01 MTR_1g108820 32.911 316 176 11 10 300 80 384 2.06e-34 129
MsG0780037556.01.T01 MTR_7g033945 63.000 100 31 1 1 94 74 173 3.74e-34 123
MsG0780037556.01.T01 MTR_5g058100 34.304 309 178 10 9 301 84 383 3.95e-34 128
MsG0780037556.01.T01 MTR_3g105060 31.511 311 183 10 3 301 80 372 5.19e-34 127
MsG0780037556.01.T01 MTR_3g107100 33.904 292 162 10 35 300 110 396 6.85e-34 128
MsG0780037556.01.T01 MTR_2g007450 35.032 314 154 13 27 300 115 418 1.25e-33 127
MsG0780037556.01.T01 MTR_3g040700 34.448 299 161 12 1 284 106 384 1.36e-33 127
MsG0780037556.01.T01 MTR_3g107160 33.333 285 164 10 30 299 107 380 2.16e-33 126
MsG0780037556.01.T01 MTR_7g081290 49.275 138 60 5 170 301 122 255 3.35e-33 122
MsG0780037556.01.T01 MTR_7g056020 35.889 287 151 11 29 294 92 366 3.62e-33 129
MsG0780037556.01.T01 MTR_1g085120 31.731 312 186 11 9 301 167 470 1.70e-32 125
MsG0780037556.01.T01 MTR_7g114710 33.013 312 169 9 3 301 79 363 1.81e-32 123
MsG0780037556.01.T01 MTR_1g085120 31.731 312 186 11 9 301 242 545 3.72e-32 125
MsG0780037556.01.T01 MTR_5g073420 30.696 316 185 13 3 301 81 379 2.00e-31 121
MsG0780037556.01.T01 MTR_3g467400 34.286 315 161 13 9 301 76 366 2.04e-31 120
MsG0780037556.01.T01 MTR_1g108800 35.179 307 166 11 10 300 51 340 2.08e-31 120
MsG0780037556.01.T01 MTR_3g107120 33.758 314 166 14 10 299 93 388 2.28e-31 121
MsG0780037556.01.T01 MTR_4g080610 36.777 242 128 11 80 302 122 357 2.47e-31 120
MsG0780037556.01.T01 MTR_1089s0010 33.228 316 185 11 2 301 73 378 3.68e-31 120
MsG0780037556.01.T01 MTR_6g040270 30.721 319 189 12 2 300 73 379 7.92e-31 119
MsG0780037556.01.T01 MTR_3g115430 49.606 127 56 3 180 301 1 124 2.76e-30 111
MsG0780037556.01.T01 MTR_2g007400 34.686 271 140 12 53 300 145 401 4.68e-30 117
MsG0780037556.01.T01 MTR_2g005990 32.692 312 158 13 23 300 100 393 1.49e-29 116
MsG0780037556.01.T01 MTR_7g007080 32.698 315 174 12 9 300 78 377 1.54e-29 116
MsG0780037556.01.T01 MTR_5g054920 34.343 198 109 7 100 284 2 191 1.97e-29 111
MsG0780037556.01.T01 MTR_3g099830 33.893 298 162 11 26 300 87 372 4.23e-29 114
MsG0780037556.01.T01 MTR_6g022520 29.968 317 201 7 1 301 1 312 1.56e-28 112
MsG0780037556.01.T01 MTR_7g060470 33.684 285 145 12 34 287 107 378 1.70e-28 113
MsG0780037556.01.T01 MTR_2g036470 31.230 317 182 12 11 302 91 396 3.78e-28 112
MsG0780037556.01.T01 MTR_6g011460 32.268 313 189 9 4 301 116 420 3.85e-28 112
MsG0780037556.01.T01 MTR_1g053025 29.553 291 166 11 35 299 109 386 5.72e-28 111
MsG0780037556.01.T01 MTR_5g073370 32.258 310 183 11 10 300 77 378 8.75e-28 111
MsG0780037556.01.T01 MTR_1g107385 32.319 263 137 10 64 300 174 421 4.97e-27 109
MsG0780037556.01.T01 MTR_5g063950 30.744 309 188 11 8 299 60 359 8.74e-27 108
MsG0780037556.01.T01 MTR_3g108560 35.556 225 128 7 1 215 83 300 3.96e-26 105
MsG0780037556.01.T01 MTR_3g107940 46.032 126 54 4 174 294 89 205 5.36e-26 105
MsG0780037556.01.T01 MTR_7g083740 30.195 308 160 11 22 302 88 367 7.07e-26 105
MsG0780037556.01.T01 MTR_7g055943 30.351 313 191 11 9 301 115 420 1.22e-25 105
MsG0780037556.01.T01 MTR_5g039450 30.962 239 131 7 81 299 131 355 4.36e-25 103
MsG0780037556.01.T01 MTR_7g007340 33.071 254 143 10 57 294 107 349 5.47e-25 103
MsG0780037556.01.T01 MTR_7g021990 35.102 245 140 9 63 290 171 413 5.48e-25 103
MsG0780037556.01.T01 MTR_5g039490 31.090 312 167 12 8 301 94 375 8.84e-25 102
MsG0780037556.01.T01 MTR_2g007360 30.897 301 163 14 34 300 83 372 1.22e-24 102
MsG0780037556.01.T01 MTR_7g021950 34.818 247 142 9 63 292 171 415 1.32e-24 103
MsG0780037556.01.T01 MTR_2g007090 32.735 223 127 8 84 301 89 293 1.61e-24 100
MsG0780037556.01.T01 MTR_5g039470 30.420 286 163 11 4 266 55 327 2.18e-24 100
MsG0780037556.01.T01 MTR_6g022360 31.641 256 157 7 62 302 156 408 2.40e-24 102
MsG0780037556.01.T01 MTR_5g045510 30.159 315 176 10 4 300 82 370 4.75e-24 100
MsG0780037556.01.T01 MTR_6g029350 34.450 209 111 10 104 300 2 196 3.37e-23 95.9
MsG0780037556.01.T01 MTR_5g031970 31.579 304 178 12 8 302 74 356 7.20e-23 97.4
MsG0780037556.01.T01 MTR_4g039440 31.418 261 154 8 62 301 155 411 1.14e-22 97.4
MsG0780037556.01.T01 MTR_4g098590 31.699 306 164 15 10 281 73 367 1.36e-22 96.7
MsG0780037556.01.T01 MTR_2g007550 36.296 135 68 3 169 299 212 332 2.61e-22 95.5
MsG0780037556.01.T01 MTR_3g026920 33.043 230 115 9 94 299 188 402 6.53e-22 95.1
MsG0780037556.01.T01 MTR_0009s0080 31.206 282 164 10 28 288 114 386 6.96e-22 95.5
MsG0780037556.01.T01 MTR_2g008310 29.818 275 151 11 48 300 115 369 1.84e-21 94.0
MsG0780037556.01.T01 MTR_6g044572 30.743 296 158 15 24 301 121 387 3.30e-21 93.2
MsG0780037556.01.T01 MTR_6g044560 30.743 296 158 15 24 301 297 563 1.29e-20 92.4
MsG0780037556.01.T01 MTR_1g043170 51.163 86 38 2 217 301 5 87 1.30e-20 84.7
MsG0780037556.01.T01 MTR_5g083890 31.004 229 126 9 84 291 174 391 1.70e-20 91.7
MsG0780037556.01.T01 MTR_7g022105 32.231 242 145 8 63 287 172 411 3.63e-20 90.5
MsG0780037556.01.T01 MTR_7g075820 30.303 231 133 9 84 300 176 392 4.21e-20 90.1
MsG0780037556.01.T01 MTR_7g033940 63.492 63 23 0 1 63 157 219 4.41e-20 87.0
MsG0780037556.01.T01 MTR_2g007500 32.283 254 131 11 27 249 91 334 1.71e-19 87.4
MsG0780037556.01.T01 MTR_8g077460 30.323 310 174 13 10 301 89 374 2.84e-19 87.4
MsG0780037556.01.T01 MTR_2g007610 38.519 135 68 3 171 300 25 149 3.57e-19 83.2
MsG0780037556.01.T01 MTR_2g008320 29.787 282 144 12 48 300 169 425 4.23e-19 87.4
MsG0780037556.01.T01 MTR_3g095630 32.584 178 104 6 125 292 4 175 4.97e-19 83.6
MsG0780037556.01.T01 MTR_7g007370 33.186 226 119 8 82 294 123 329 5.32e-19 86.3
MsG0780037556.01.T01 MTR_3g110010 33.906 233 118 11 39 239 194 422 1.63e-18 86.3
MsG0780037556.01.T01 MTR_3g110010 30.455 220 126 9 1 208 76 280 9.97e-14 72.0
MsG0780037556.01.T01 MTR_4g056040 26.269 335 171 9 14 274 113 445 1.66e-18 85.9
MsG0780037556.01.T01 MTR_3g106960 38.583 127 66 4 174 296 74 192 1.80e-18 83.2
MsG0780037556.01.T01 MTR_5g033200 33.793 145 86 4 155 292 31 172 5.73e-18 80.5
MsG0780037556.01.T01 MTR_1g059860 28.523 298 150 11 28 274 38 323 5.80e-18 83.2
MsG0780037556.01.T01 MTR_4g056020 26.426 333 165 11 14 270 83 411 3.47e-17 82.0
MsG0780037556.01.T01 MTR_7g056280 38.136 118 61 3 162 270 432 546 3.83e-17 82.0
MsG0780037556.01.T01 MTR_3g113785 46.316 95 37 2 174 260 4 92 6.37e-17 75.1
MsG0780037556.01.T01 MTR_3g113770 29.268 205 83 5 64 260 184 334 7.25e-17 80.1
MsG0780037556.01.T01 MTR_1377s0010 36.029 136 73 6 155 285 11 137 1.26e-16 75.5
MsG0780037556.01.T01 MTR_3g108140 46.914 81 42 1 223 302 68 148 3.97e-16 75.1
MsG0780037556.01.T01 MTR_5g024060 33.613 119 77 1 174 292 82 198 5.14e-16 76.6
MsG0780037556.01.T01 MTR_5g008910 41.573 89 48 2 214 301 216 301 5.49e-16 77.4
MsG0780037556.01.T01 MTR_4g056030 26.586 331 167 10 14 270 83 411 7.24e-16 78.2
MsG0780037556.01.T01 MTR_4g055580 26.190 336 161 11 14 270 211 538 7.83e-16 78.2
MsG0780037556.01.T01 MTR_3g071790 28.796 191 113 7 90 264 19 202 9.55e-16 74.7
MsG0780037556.01.T01 MTR_1g108820 29.956 227 129 8 10 213 80 299 7.06e-15 74.3
MsG0780037556.01.T01 MTR_8g062310 25.145 346 191 11 5 301 101 427 8.52e-15 74.7
MsG0780037556.01.T01 MTR_7g034090 35.000 140 72 5 171 301 182 311 1.69e-14 73.9
MsG0780037556.01.T01 MTR_5g073340 31.088 193 110 7 66 239 112 300 2.88e-14 72.4
MsG0780037556.01.T01 MTR_1g085120 30.942 223 129 9 9 213 254 469 3.21e-14 73.2
MsG0780037556.01.T01 MTR_7g056447 33.146 178 91 6 1 172 72 227 3.23e-14 72.0
MsG0780037556.01.T01 MTR_8g014210 37.168 113 70 1 189 301 119 230 3.93e-14 70.9
MsG0780037556.01.T01 MTR_3g094980 33.036 112 68 2 164 270 8 117 4.47e-14 69.3
MsG0780037556.01.T01 MTR_8g058970 37.500 112 66 2 194 301 365 476 5.36e-14 72.4
MsG0780037556.01.T01 MTR_5g084070 30.870 230 124 8 85 294 205 419 2.68e-13 70.1
MsG0780037556.01.T01 MTR_4g096730 45.000 80 40 2 223 301 12 88 4.72e-13 64.7
MsG0780037556.01.T01 MTR_7g022060 29.114 237 122 8 63 294 127 322 5.25e-13 68.9
MsG0780037556.01.T01 MTR_2g007530 36.508 126 57 3 174 299 232 334 8.01e-13 68.6
MsG0780037556.01.T01 MTR_7g021930 29.114 237 122 8 63 294 127 322 3.57e-12 66.2
MsG0780037556.01.T01 MTR_7g021970 29.114 237 122 8 63 294 127 322 3.57e-12 66.2
MsG0780037556.01.T01 MTR_5g092960 37.391 115 63 4 193 301 350 461 6.04e-12 66.2
MsG0780037556.01.T01 MTR_8g062010 37.594 133 72 4 174 301 303 429 9.20e-12 65.5
MsG0780037556.01.T01 MTR_8g070950 27.635 351 185 14 10 301 93 433 1.23e-11 65.1
MsG0780037556.01.T01 MTR_1g053050 36.585 82 48 1 218 299 35 112 1.33e-11 61.2
MsG0780037556.01.T01 MTR_7g056417 35.570 149 70 6 64 191 15 158 1.42e-11 62.0
MsG0780037556.01.T01 MTR_8g058965 24.728 368 198 14 10 301 91 455 1.58e-11 65.1
MsG0780037556.01.T01 MTR_4g024710 35.115 131 75 3 174 301 223 346 1.78e-11 64.3
MsG0780037556.01.T01 MTR_8g467800 33.333 144 75 5 170 301 318 452 2.77e-11 64.3
MsG0780037556.01.T01 MTR_8g014260 35.659 129 78 4 174 301 326 450 3.58e-11 63.9
MsG0780037556.01.T01 MTR_6g022410 44.118 68 37 1 235 301 39 106 3.77e-11 59.7
MsG0780037556.01.T01 MTR_5g044790 32.857 140 61 4 195 302 302 440 4.10e-11 63.5
MsG0780037556.01.T01 MTR_6g013160 28.309 272 150 10 5 232 108 378 5.73e-11 63.2
MsG0780037556.01.T01 MTR_7g075800 25.705 319 167 17 13 268 82 393 7.64e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037556.01.T01 AT1G32375 27.557 352 191 13 10 301 74 421 1.24e-24 103
MsG0780037556.01.T01 AT1G32375 27.557 352 191 13 10 301 74 421 1.24e-24 103
MsG0780037556.01.T01 AT5G56370 29.545 352 183 12 10 301 74 420 3.49e-24 101
MsG0780037556.01.T01 AT5G56370 29.545 352 183 12 10 301 74 420 3.49e-24 101
MsG0780037556.01.T01 AT4G26340 30.286 350 181 12 10 301 74 418 3.39e-23 99.0
MsG0780037556.01.T01 AT3G50710 28.857 350 180 15 15 299 79 424 7.92e-17 80.9
MsG0780037556.01.T01 AT3G52680 27.424 361 184 12 10 301 93 444 2.18e-16 79.7
MsG0780037556.01.T01 AT5G56420 27.171 357 198 14 1 301 71 421 3.28e-16 79.0
MsG0780037556.01.T01 AT5G56420 27.171 357 198 14 1 301 71 421 3.28e-16 79.0
MsG0780037556.01.T01 AT5G56420 27.171 357 198 14 1 301 71 421 3.28e-16 79.0
MsG0780037556.01.T01 AT5G62970 25.496 353 198 11 10 301 100 448 1.04e-15 77.4
MsG0780037556.01.T01 AT5G56380 31.061 132 85 3 173 301 310 438 1.34e-15 77.0
MsG0780037556.01.T01 AT5G56380 31.061 132 85 3 173 301 310 438 1.34e-15 77.0
MsG0780037556.01.T01 AT5G56390 26.359 368 191 15 2 301 69 424 2.38e-15 76.3
MsG0780037556.01.T01 AT5G38590 24.927 341 193 11 2 287 69 401 2.41e-15 76.3
MsG0780037556.01.T01 AT4G26350 26.667 345 185 10 23 301 85 427 3.56e-15 75.9
MsG0780037556.01.T01 AT5G38580 33.103 145 90 3 163 302 22 164 2.62e-14 70.1
MsG0780037556.01.T01 AT5G44850 28.621 290 170 13 27 294 49 323 4.10e-14 72.0
MsG0780037556.01.T01 AT4G10400 28.775 351 188 15 2 301 69 408 1.17e-13 71.2
MsG0780037556.01.T01 AT4G10400 28.775 351 188 15 2 301 69 408 1.17e-13 71.2
MsG0780037556.01.T01 AT4G10400 28.775 351 188 15 2 301 69 408 1.17e-13 71.2
MsG0780037556.01.T01 AT4G00160 27.451 357 190 15 10 301 89 441 3.70e-13 70.1
MsG0780037556.01.T01 AT5G38565 34.228 149 80 5 160 300 201 339 1.10e-12 68.2
MsG0780037556.01.T01 AT5G50270 27.434 339 177 15 10 284 75 408 3.65e-12 66.6
MsG0780037556.01.T01 AT5G56810 29.392 296 144 11 64 299 141 431 6.03e-12 66.2
MsG0780037556.01.T01 AT3G54910 26.331 357 193 15 2 301 29 372 7.00e-12 65.9
MsG0780037556.01.T01 AT3G54910 26.331 357 193 15 2 301 29 372 7.00e-12 65.9
MsG0780037556.01.T01 AT3G54910 26.331 357 193 15 2 301 29 372 7.00e-12 65.9
MsG0780037556.01.T01 AT1G50980 27.273 297 189 11 7 290 76 358 4.51e-11 63.5

Find 71 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATATCAATCAATTTATT+TGG 0.089474 7:+28378227 MsG0780037556.01.T01:CDS
TTTGAAGCTTAGGGCAATTT+TGG 0.160396 7:-28378832 None:intergenic
TGTGGTGTTTGACAGCTTTA+TGG 0.220610 7:+28378944 MsG0780037556.01.T01:intron
AGCTTATATTTGAATCAAAT+TGG 0.245379 7:+28378777 MsG0780037556.01.T01:CDS
AAGAGATTTGAATAAGCTTA+TGG 0.253453 7:+28378444 MsG0780037556.01.T01:CDS
CAAGGCTCTCCATTTGAAAA+AGG 0.254682 7:+28378411 MsG0780037556.01.T01:CDS
ACATTTATAAATGTATAAAA+TGG 0.294381 7:+28378572 MsG0780037556.01.T01:CDS
GAACTTAATCTAATACTAAA+TGG 0.298219 7:-28378307 None:intergenic
CACATTTGAGGCGGAATGAT+TGG 0.333602 7:-28378180 None:intergenic
GAATCTCATCTTAGACTTGT+TGG 0.342326 7:+28378273 MsG0780037556.01.T01:CDS
TGATATCAATCAATTTATTT+GGG 0.343264 7:+28378228 MsG0780037556.01.T01:CDS
AATGTTAGCTGTAACCAAAC+TGG 0.349529 7:-28378554 None:intergenic
AATGGAATATTTCATTTGCT+TGG 0.355632 7:+28378590 MsG0780037556.01.T01:CDS
AGTTGAGAATAAAGACATTT+GGG 0.373321 7:-28379010 None:intergenic
TAGTTGAGAATAAAGACATT+TGG 0.387387 7:-28379011 None:intergenic
ATTTAAATACCTACCCAGTT+TGG 0.395147 7:+28378540 MsG0780037556.01.T01:CDS
CAGATAAATCCATTAGCATT+TGG 0.409550 7:-28379165 None:intergenic
AATCTCATCTTAGACTTGTT+GGG 0.417431 7:+28378274 MsG0780037556.01.T01:CDS
AGACATTTGGGAACTATTTG+TGG 0.425300 7:-28378998 None:intergenic
TAGATGCAATTCCTCTAGAA+TGG 0.438785 7:-28378476 None:intergenic
AAAGTTTCTGCGTGTACAAC+TGG 0.439039 7:+28378624 MsG0780037556.01.T01:CDS
ATTGGCGTACCAAATGGAAT+TGG 0.442997 7:+28378795 MsG0780037556.01.T01:CDS
ATGAATGAAAGTTTACATAT+AGG 0.443623 7:-28379205 None:intergenic
AATCAAATTGGCGTACCAAA+TGG 0.448646 7:+28378789 MsG0780037556.01.T01:CDS
TTGTACCAACCAATTCCATT+TGG 0.473258 7:-28378804 None:intergenic
CCTCAAATGTGGTACTTCCC+CGG 0.482086 7:+28378192 MsG0780037556.01.T01:CDS
TGGATTTATCTGTGTGTCCA+AGG 0.484784 7:+28379176 MsG0780037556.01.T01:CDS
CTCAAATGTGGTACTTCCCC+GGG 0.489887 7:+28378193 MsG0780037556.01.T01:CDS
AATGATTGGATGGGCAATGT+GGG 0.491272 7:-28378166 None:intergenic
TATCATGTAGATTGCATCCC+GGG 0.495391 7:-28378210 None:intergenic
TGGTAGTGATGAGGACGATT+GGG 0.497885 7:+28378970 MsG0780037556.01.T01:CDS
ATGGTAGTGATGAGGACGAT+TGG 0.502082 7:+28378969 MsG0780037556.01.T01:CDS
GGATTTATCTGTGTGTCCAA+GGG 0.506175 7:+28379177 MsG0780037556.01.T01:CDS
TGTCACTGTGCGCAGTAAGT+TGG 0.507051 7:-28379107 None:intergenic
CCGGGGAAGTACCACATTTG+AGG 0.514518 7:-28378192 None:intergenic
CAAATGGAGAGCCTTGAGTA+GGG 0.519786 7:-28378404 None:intergenic
AGATGCAATTCCTCTAGAAT+GGG 0.520829 7:-28378475 None:intergenic
TTTAGTTAAATCCTCAGAAG+AGG 0.532757 7:-28379137 None:intergenic
TTTGAGGCGGAATGATTGGA+TGG 0.533442 7:-28378176 None:intergenic
GATTTGAATAAGCTTATGGA+GGG 0.535898 7:+28378448 MsG0780037556.01.T01:CDS
GTTGAAGAATATAGTTGTGG+AGG 0.543228 7:+28378360 MsG0780037556.01.T01:CDS
GACAATTAATGCCTCTTCTG+AGG 0.546687 7:+28379126 MsG0780037556.01.T01:CDS
TCAAATGGAGAGCCTTGAGT+AGG 0.555827 7:-28378405 None:intergenic
AGATTTGAATAAGCTTATGG+AGG 0.572241 7:+28378447 MsG0780037556.01.T01:CDS
ACATAAGGAAAACCTTGTGA+AGG 0.579671 7:-28378862 None:intergenic
GAATGATTGGATGGGCAATG+TGG 0.584711 7:-28378167 None:intergenic
ATTTGGGCTGCTGTGCAAAG+AGG 0.584733 7:+28378244 MsG0780037556.01.T01:CDS
TAAGTTGAAGAATATAGTTG+TGG 0.594110 7:+28378357 MsG0780037556.01.T01:CDS
CAATCATTCCGCCTCAAATG+TGG 0.594779 7:+28378181 MsG0780037556.01.T01:CDS
TTGAGGCGGAATGATTGGAT+GGG 0.595298 7:-28378175 None:intergenic
TACAATTTCTCGAAACCTCG+TGG 0.600422 7:+28378330 MsG0780037556.01.T01:CDS
ATGTTAGCTGTAACCAAACT+GGG 0.604526 7:-28378553 None:intergenic
CATAAGGAAAACCTTGTGAA+GGG 0.609317 7:-28378861 None:intergenic
TCAAAATCTTACCCTTCACA+AGG 0.611775 7:+28378850 MsG0780037556.01.T01:CDS
TTACATATAGGAGAGCCCCT+TGG 0.614946 7:-28379193 None:intergenic
AAATGGAGAGCCTTGAGTAG+GGG 0.620368 7:-28378403 None:intergenic
GTTTGACAGCTTTATGGACA+TGG 0.625653 7:+28378950 MsG0780037556.01.T01:intron
ATATCATGTAGATTGCATCC+CGG 0.625933 7:-28378211 None:intergenic
GCGTACCAAATGGAATTGGT+TGG 0.626150 7:+28378799 MsG0780037556.01.T01:CDS
ATGTCTTTGAAAATCAGTAA+TGG 0.639106 7:+28378514 MsG0780037556.01.T01:CDS
GGAGGGGTGTCCCATTCTAG+AGG 0.644739 7:+28378465 MsG0780037556.01.T01:CDS
ATTTGAATAAGCTTATGGAG+GGG 0.645308 7:+28378449 MsG0780037556.01.T01:CDS
GATTTATCTGTGTGTCCAAG+GGG 0.645548 7:+28379178 MsG0780037556.01.T01:CDS
TAGCTGTAACCAAACTGGGT+AGG 0.652949 7:-28378549 None:intergenic
ATGATTGGATGGGCAATGTG+GGG 0.657098 7:-28378165 None:intergenic
TTATGGACATGGTAGTGATG+AGG 0.664079 7:+28378961 MsG0780037556.01.T01:CDS
GGGAAGTACCACATTTGAGG+CGG 0.672790 7:-28378189 None:intergenic
AGTAGGGGAAAATAGACTTG+AGG 0.676320 7:-28378388 None:intergenic
TGCTCGCTTATAGATTACAA+AGG 0.689162 7:+28379040 MsG0780037556.01.T01:CDS
ATCATGTAGATTGCATCCCG+GGG 0.708029 7:-28378209 None:intergenic
TCTTCAACTTAAGAACCACG+AGG 0.770441 7:-28378345 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTTATAAATGTATAAAA+TGG + Chr7:28378572-28378591 MsG0780037556.01.T01:CDS 10.0%
!! ATGATATCAATCAATTTATT+TGG + Chr7:28378227-28378246 MsG0780037556.01.T01:CDS 15.0%
!! TGATATCAATCAATTTATTT+GGG + Chr7:28378228-28378247 MsG0780037556.01.T01:CDS 15.0%
!!! CTAATTTATATTTTTTTCTG+TGG + Chr7:28378926-28378945 MsG0780037556.01.T01:intron 15.0%
!! AATAAATCATGATTGACATA+AGG - Chr7:28378880-28378899 None:intergenic 20.0%
!! AGCTTATATTTGAATCAAAT+TGG + Chr7:28378777-28378796 MsG0780037556.01.T01:CDS 20.0%
!! ATGAATGAAAGTTTACATAT+AGG - Chr7:28379208-28379227 None:intergenic 20.0%
!! GAACTTAATCTAATACTAAA+TGG - Chr7:28378310-28378329 None:intergenic 20.0%
!!! TAAAGATAACCTTTTTCAAA+TGG - Chr7:28378423-28378442 None:intergenic 20.0%
! AAGAGATTTGAATAAGCTTA+TGG + Chr7:28378444-28378463 MsG0780037556.01.T01:CDS 25.0%
! AATGGAATATTTCATTTGCT+TGG + Chr7:28378590-28378609 MsG0780037556.01.T01:CDS 25.0%
! AGTTGAGAATAAAGACATTT+GGG - Chr7:28379013-28379032 None:intergenic 25.0%
! GTTCAACTTATTGTAACTTT+AGG - Chr7:28378669-28378688 None:intergenic 25.0%
! TAGTTGAGAATAAAGACATT+TGG - Chr7:28379014-28379033 None:intergenic 25.0%
!! ATGTCTTTGAAAATCAGTAA+TGG + Chr7:28378514-28378533 MsG0780037556.01.T01:CDS 25.0%
!! TAAGTTGAAGAATATAGTTG+TGG + Chr7:28378357-28378376 MsG0780037556.01.T01:CDS 25.0%
!! TTTTCATAACTTAACTCACA+TGG + Chr7:28378754-28378773 MsG0780037556.01.T01:CDS 25.0%
!!! TAAAAAGTACCAAATGCTAA+TGG + Chr7:28379156-28379175 MsG0780037556.01.T01:CDS 25.0%
AGATTTGAATAAGCTTATGG+AGG + Chr7:28378447-28378466 MsG0780037556.01.T01:CDS 30.0%
ATTTAAATACCTACCCAGTT+TGG + Chr7:28378540-28378559 MsG0780037556.01.T01:CDS 30.0%
ATTTGAATAAGCTTATGGAG+GGG + Chr7:28378449-28378468 MsG0780037556.01.T01:CDS 30.0%
CAGATAAATCCATTAGCATT+TGG - Chr7:28379168-28379187 None:intergenic 30.0%
GATTTGAATAAGCTTATGGA+GGG + Chr7:28378448-28378467 MsG0780037556.01.T01:CDS 30.0%
TTTAGTTAAATCCTCAGAAG+AGG - Chr7:28379140-28379159 None:intergenic 30.0%
! AATCTCATCTTAGACTTGTT+GGG + Chr7:28378274-28378293 MsG0780037556.01.T01:CDS 30.0%
AATCAAATTGGCGTACCAAA+TGG + Chr7:28378789-28378808 MsG0780037556.01.T01:CDS 35.0%
AATGTTAGCTGTAACCAAAC+TGG - Chr7:28378557-28378576 None:intergenic 35.0%
ACATAAGGAAAACCTTGTGA+AGG - Chr7:28378865-28378884 None:intergenic 35.0%
AGACATTTGGGAACTATTTG+TGG - Chr7:28379001-28379020 None:intergenic 35.0%
ATATCATGTAGATTGCATCC+CGG - Chr7:28378214-28378233 None:intergenic 35.0%
ATGTTAGCTGTAACCAAACT+GGG - Chr7:28378556-28378575 None:intergenic 35.0%
CATAAGGAAAACCTTGTGAA+GGG - Chr7:28378864-28378883 None:intergenic 35.0%
GAATCTCATCTTAGACTTGT+TGG + Chr7:28378273-28378292 MsG0780037556.01.T01:CDS 35.0%
GTTGAAGAATATAGTTGTGG+AGG + Chr7:28378360-28378379 MsG0780037556.01.T01:CDS 35.0%
TCAAAATCTTACCCTTCACA+AGG + Chr7:28378850-28378869 MsG0780037556.01.T01:CDS 35.0%
TGCTCGCTTATAGATTACAA+AGG + Chr7:28379040-28379059 MsG0780037556.01.T01:CDS 35.0%
TTGTACCAACCAATTCCATT+TGG - Chr7:28378807-28378826 None:intergenic 35.0%
! TAGATGCAATTCCTCTAGAA+TGG - Chr7:28378479-28378498 None:intergenic 35.0%
!! AGATGCAATTCCTCTAGAAT+GGG - Chr7:28378478-28378497 None:intergenic 35.0%
!! TTTGAAGCTTAGGGCAATTT+TGG - Chr7:28378835-28378854 None:intergenic 35.0%
!!! AGGGTAAGATTTTGAAGCTT+AGG - Chr7:28378845-28378864 None:intergenic 35.0%
!!! GGGTAAGATTTTGAAGCTTA+GGG - Chr7:28378844-28378863 None:intergenic 35.0%
AAAGTTTCTGCGTGTACAAC+TGG + Chr7:28378624-28378643 MsG0780037556.01.T01:CDS 40.0%
AATGATTGGATGGGCAATGT+GGG - Chr7:28378169-28378188 None:intergenic 40.0%
AGTAGGGGAAAATAGACTTG+AGG - Chr7:28378391-28378410 None:intergenic 40.0%
ATTGGCGTACCAAATGGAAT+TGG + Chr7:28378795-28378814 MsG0780037556.01.T01:CDS 40.0%
CAAGGCTCTCCATTTGAAAA+AGG + Chr7:28378411-28378430 MsG0780037556.01.T01:CDS 40.0%
GACAATTAATGCCTCTTCTG+AGG + Chr7:28379126-28379145 MsG0780037556.01.T01:CDS 40.0%
GATTTATCTGTGTGTCCAAG+GGG + Chr7:28379178-28379197 MsG0780037556.01.T01:CDS 40.0%
GGATTTATCTGTGTGTCCAA+GGG + Chr7:28379177-28379196 MsG0780037556.01.T01:CDS 40.0%
TACAATTTCTCGAAACCTCG+TGG + Chr7:28378330-28378349 MsG0780037556.01.T01:CDS 40.0%
TATCATGTAGATTGCATCCC+GGG - Chr7:28378213-28378232 None:intergenic 40.0%
TCTTCAACTTAAGAACCACG+AGG - Chr7:28378348-28378367 None:intergenic 40.0%
TGGATTTATCTGTGTGTCCA+AGG + Chr7:28379176-28379195 MsG0780037556.01.T01:CDS 40.0%
TGTGGTGTTTGACAGCTTTA+TGG + Chr7:28378944-28378963 MsG0780037556.01.T01:intron 40.0%
TTTGTCTTTGTAACTCACGC+AGG + Chr7:28378709-28378728 MsG0780037556.01.T01:intron 40.0%
! AGTCTATTTTCCCCTACTCA+AGG + Chr7:28378393-28378412 MsG0780037556.01.T01:CDS 40.0%
! GTTTGACAGCTTTATGGACA+TGG + Chr7:28378950-28378969 MsG0780037556.01.T01:intron 40.0%
! TTATGGACATGGTAGTGATG+AGG + Chr7:28378961-28378980 MsG0780037556.01.T01:CDS 40.0%
AAATGGAGAGCCTTGAGTAG+GGG - Chr7:28378406-28378425 None:intergenic 45.0%
ATCATGTAGATTGCATCCCG+GGG - Chr7:28378212-28378231 None:intergenic 45.0%
ATGATTGGATGGGCAATGTG+GGG - Chr7:28378168-28378187 None:intergenic 45.0%
CAAATGGAGAGCCTTGAGTA+GGG - Chr7:28378407-28378426 None:intergenic 45.0%
CAATCATTCCGCCTCAAATG+TGG + Chr7:28378181-28378200 MsG0780037556.01.T01:CDS 45.0%
GAATGATTGGATGGGCAATG+TGG - Chr7:28378170-28378189 None:intergenic 45.0%
GCGTACCAAATGGAATTGGT+TGG + Chr7:28378799-28378818 MsG0780037556.01.T01:CDS 45.0%
TAGCTGTAACCAAACTGGGT+AGG - Chr7:28378552-28378571 None:intergenic 45.0%
TCAAATGGAGAGCCTTGAGT+AGG - Chr7:28378408-28378427 None:intergenic 45.0%
TTACATATAGGAGAGCCCCT+TGG - Chr7:28379196-28379215 None:intergenic 45.0%
TTGAGGCGGAATGATTGGAT+GGG - Chr7:28378178-28378197 None:intergenic 45.0%
! ATGGTAGTGATGAGGACGAT+TGG + Chr7:28378969-28378988 MsG0780037556.01.T01:CDS 45.0%
! CACATTTGAGGCGGAATGAT+TGG - Chr7:28378183-28378202 None:intergenic 45.0%
! TGGTAGTGATGAGGACGATT+GGG + Chr7:28378970-28378989 MsG0780037556.01.T01:CDS 45.0%
! TTTGAGGCGGAATGATTGGA+TGG - Chr7:28378179-28378198 None:intergenic 45.0%
ATTTGGGCTGCTGTGCAAAG+AGG + Chr7:28378244-28378263 MsG0780037556.01.T01:CDS 50.0%
CCTCAAATGTGGTACTTCCC+CGG + Chr7:28378192-28378211 MsG0780037556.01.T01:CDS 50.0%
CTCAAATGTGGTACTTCCCC+GGG + Chr7:28378193-28378212 MsG0780037556.01.T01:CDS 50.0%
TGTCACTGTGCGCAGTAAGT+TGG - Chr7:28379110-28379129 None:intergenic 50.0%
!! GGGAAGTACCACATTTGAGG+CGG - Chr7:28378192-28378211 None:intergenic 50.0%
!! CCGGGGAAGTACCACATTTG+AGG - Chr7:28378195-28378214 None:intergenic 55.0%
!! GGAGGGGTGTCCCATTCTAG+AGG + Chr7:28378465-28378484 MsG0780037556.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 28378151 28379231 28378151 ID=MsG0780037556.01;Name=MsG0780037556.01
Chr7 mRNA 28378151 28379231 28378151 ID=MsG0780037556.01.T01;Parent=MsG0780037556.01;Name=MsG0780037556.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|1|1|3|0|302
Chr7 exon 28378151 28378645 28378151 ID=MsG0780037556.01.T01:exon:13148;Parent=MsG0780037556.01.T01
Chr7 exon 28378731 28378871 28378731 ID=MsG0780037556.01.T01:exon:13149;Parent=MsG0780037556.01.T01
Chr7 exon 28378959 28379231 28378959 ID=MsG0780037556.01.T01:exon:13150;Parent=MsG0780037556.01.T01
Chr7 CDS 28378151 28378645 28378151 ID=MsG0780037556.01.T01:cds;Parent=MsG0780037556.01.T01
Chr7 CDS 28378731 28378871 28378731 ID=MsG0780037556.01.T01:cds;Parent=MsG0780037556.01.T01
Chr7 CDS 28378959 28379231 28378959 ID=MsG0780037556.01.T01:cds;Parent=MsG0780037556.01.T01
Gene Sequence

>MsG0780037556.01.T01

ATGCTCATGCGTGACCCCACATTGCCCATCCAATCATTCCGCCTCAAATGTGGTACTTCCCCGGGATGCAATCTACATGATATCAATCAATTTATTTGGGCTGCTGTGCAAAGAGGAATTCAGAATCTCATCTTAGACTTGTTGGGAACTGATAGACCATTTAGTATTAGATTAAGTTCTACAATTTCTCGAAACCTCGTGGTTCTTAAGTTGAAGAATATAGTTGTGGAGGTTCTTCCTCAAGTCTATTTTCCCCTACTCAAGGCTCTCCATTTGAAAAAGGTTATCTTTAAAAGAGATTTGAATAAGCTTATGGAGGGGTGTCCCATTCTAGAGGAATTGCATCTAACTTCTGTAGTATGCATGTCTTTGAAAATCAGTAATGGAGAATTTAAATACCTACCCAGTTTGGTTACAGCTAACATTTATAAATGTATAAAATGGAATATTTCATTTGCTTGGATTTGTAATGCAAAGTTTCTGCGTGTACAACTGGATGAACAGCAACAAGTTCACACTTTTCATAACTTAACTCACATGGAGCTTATATTTGAATCAAATTGGCGTACCAAATGGAATTGGTTGGTACAAGTGCTCCAAAATTGCCCTAAGCTTCAAAATCTTACCCTTCACAAGCTTTATGGACATGGTAGTGATGAGGACGATTGGGAAGAACCACAAATAGTTCCCAAATGTCTTTATTCTCAACTAAGAACATGCTCGCTTATAGATTACAAAGGCACGAAGTGTGAGCTTCAGTTTGCAGAGTATGTTTTGAAGAATGCCAACTTACTGCGCACAGTGACAATTAATGCCTCTTCTGAGGATTTAACTAAAAAGTACCAAATGCTAATGGATTTATCTGTGTGTCCAAGGGGCTCTCCTATATGTAAACTTTCATTCATTTGA
Protein sequence

>MsG0780037556.01.T01

MLMRDPTLPIQSFRLKCGTSPGCNLHDINQFIWAAVQRGIQNLILDLLGTDRPFSIRLSSTISRNLVVLKLKNIVVEVLPQVYFPLLKALHLKKVIFKRDLNKLMEGCPILEELHLTSVVCMSLKISNGEFKYLPSLVTANIYKCIKWNISFAWICNAKFLRVQLDEQQQVHTFHNLTHMELIFESNWRTKWNWLVQVLQNCPKLQNLTLHKLYGHGSDEDDWEEPQIVPKCLYSQLRTCSLIDYKGTKCELQFAEYVLKNANLLRTVTINASSEDLTKKYQMLMDLSVCPRGSPICKLSFI*