Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042131.01.T01 | XP_003626950.1 | 96.33 | 109 | 4 | 0 | 14 | 122 | 108 | 216 | 9.31E-65 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042131.01.T01 | G7LF68 | 96.330 | 109 | 4 | 0 | 14 | 122 | 108 | 216 | 4.45e-65 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000575.01 | MsG0880042131.01 | 0.847914 | 8.551505e-60 | 1.543210e-56 |
| MsG0180000614.01 | MsG0880042131.01 | 0.831750 | 1.398126e-55 | 1.531520e-52 |
| MsG0180000615.01 | MsG0880042131.01 | 0.821852 | 3.238458e-53 | 2.665806e-50 |
| MsG0180000743.01 | MsG0880042131.01 | 0.859334 | 4.445133e-63 | 1.169819e-59 |
| MsG0180002504.01 | MsG0880042131.01 | 0.802659 | 5.050178e-49 | 2.487734e-46 |
| MsG0180004509.01 | MsG0880042131.01 | 0.810469 | 1.134819e-50 | 6.846260e-48 |
| MsG0180004861.01 | MsG0880042131.01 | 0.807542 | 4.803293e-50 | 2.682574e-47 |
| MsG0180005334.01 | MsG0880042131.01 | 0.803972 | 2.701003e-49 | 1.376093e-46 |
| MsG0280006422.01 | MsG0880042131.01 | 0.803327 | 3.675573e-49 | 1.841730e-46 |
| MsG0780041223.01 | MsG0880042131.01 | 0.812929 | 3.310249e-51 | 2.132034e-48 |
| MsG0780041224.01 | MsG0880042131.01 | 0.812083 | 5.068246e-51 | 3.191709e-48 |
| MsG0780041462.01 | MsG0880042131.01 | 0.812502 | 4.105670e-51 | 2.614888e-48 |
| MsG0880041967.01 | MsG0880042131.01 | 0.821874 | 3.201711e-53 | 2.637099e-50 |
| MsG0880042131.01 | MsG0880043477.01 | 0.806108 | 9.654520e-50 | 5.196323e-47 |
| MsG0880042131.01 | MsG0880046126.01 | 0.829917 | 3.936173e-55 | 4.085341e-52 |
| MsG0380012073.01 | MsG0880042131.01 | 0.807488 | 4.932097e-50 | 2.750714e-47 |
| MsG0480021159.01 | MsG0880042131.01 | 0.818507 | 1.890341e-52 | 1.417716e-49 |
| MsG0480021453.01 | MsG0880042131.01 | 0.832943 | 7.082765e-56 | 8.036657e-53 |
| MsG0480022223.01 | MsG0880042131.01 | 0.812521 | 4.066051e-51 | 2.591002e-48 |
| MsG0480022790.01 | MsG0880042131.01 | 0.826514 | 2.602546e-54 | 2.445912e-51 |
| MsG0480022960.01 | MsG0880042131.01 | 0.811010 | 8.670917e-51 | 5.307644e-48 |
| MsG0480023009.01 | MsG0880042131.01 | 0.809911 | 1.496955e-50 | 8.899805e-48 |
| MsG0480023771.01 | MsG0880042131.01 | 0.863650 | 2.139680e-64 | 6.534974e-61 |
| MsG0580024468.01 | MsG0880042131.01 | 0.813063 | 3.094522e-51 | 2.000245e-48 |
| MsG0380015027.01 | MsG0880042131.01 | 0.832713 | 8.080851e-56 | 9.106481e-53 |
| MsG0380016559.01 | MsG0880042131.01 | 0.838366 | 3.000720e-57 | 4.010293e-54 |
| MsG0380016560.01 | MsG0880042131.01 | 0.821670 | 3.569311e-53 | 2.922794e-50 |
| MsG0380016775.01 | MsG0880042131.01 | 0.836836 | 7.409973e-57 | 9.450268e-54 |
| MsG0380016860.01 | MsG0880042131.01 | 0.835640 | 1.492091e-56 | 1.835555e-53 |
| MsG0380017382.01 | MsG0880042131.01 | 0.858671 | 7.018118e-63 | 1.805249e-59 |
| MsG0280006483.01 | MsG0880042131.01 | 0.849661 | 2.801824e-60 | 5.350321e-57 |
| MsG0280007787.01 | MsG0880042131.01 | 0.817263 | 3.610908e-52 | 2.616932e-49 |
| MsG0780036546.01 | MsG0880042131.01 | 0.800733 | 1.255113e-48 | 5.887907e-46 |
| MsG0780038400.01 | MsG0880042131.01 | 0.822705 | 2.053296e-53 | 1.731736e-50 |
| MsG0780039844.01 | MsG0880042131.01 | 0.803880 | 2.822491e-49 | 1.434620e-46 |
| MsG0780039848.01 | MsG0880042131.01 | 0.818521 | 1.876971e-52 | 1.408237e-49 |
| MsG0780039998.01 | MsG0880042131.01 | 0.803895 | 2.802156e-49 | 1.424854e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042131.01.T01 | MTR_8g012410 | 96.330 | 109 | 4 | 0 | 14 | 122 | 108 | 216 | 1.13e-68 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTGACCACGGGAGGATTC+AGG | 0.326088 | 8:+4173658 | None:intergenic |
| GAAGATTTCACGCCGGGTTT+CGG | 0.336714 | 8:-4173893 | MsG0880042131.01.T01:CDS |
| TCCTCCCGTGGTCACAGCTC+TGG | 0.390004 | 8:-4173652 | MsG0880042131.01.T01:CDS |
| AGAGCGAAGAGCTCAATATC+TGG | 0.448132 | 8:-4173736 | MsG0880042131.01.T01:CDS |
| GTGCTCTAGGATCAGGGTAA+TGG | 0.467925 | 8:+4173866 | None:intergenic |
| CAGGTGGAAGATTTCACGCC+GGG | 0.486115 | 8:-4173899 | MsG0880042131.01.T01:CDS |
| GAAATTATTTCTCTTTCCAC+AGG | 0.492064 | 8:-4173918 | MsG0880042131.01.T01:CDS |
| CCACGGGAGGATTCAGGTTG+AGG | 0.507927 | 8:+4173664 | None:intergenic |
| GTATCTTCTGATCATTCTGA+TGG | 0.539185 | 8:-4173619 | MsG0880042131.01.T01:CDS |
| AAACGCCAGAGCTGTGACCA+CGG | 0.543786 | 8:+4173647 | None:intergenic |
| AACGCCAGAGCTGTGACCAC+GGG | 0.547775 | 8:+4173648 | None:intergenic |
| ATCAAAGAGAAGAAAACATA+CGG | 0.554954 | 8:-4173590 | MsG0880042131.01.T01:CDS |
| ATTATTTCTCTTTCCACAGG+TGG | 0.556329 | 8:-4173915 | MsG0880042131.01.T01:CDS |
| TTTCGAGTGCTCTAGGATCA+GGG | 0.564827 | 8:+4173860 | None:intergenic |
| GCCAGAGCTGTGACCACGGG+AGG | 0.588427 | 8:+4173651 | None:intergenic |
| GGTAATGGACAACCGAAACC+CGG | 0.610886 | 8:+4173881 | None:intergenic |
| ATTGCAAAATTCTATAGCTG+CGG | 0.618529 | 8:-4173809 | MsG0880042131.01.T01:CDS |
| TCTTCTGATCATTCTGATGG+AGG | 0.621619 | 8:-4173616 | MsG0880042131.01.T01:CDS |
| GGCGTGAAATCTTCCACCTG+TGG | 0.635136 | 8:+4173902 | None:intergenic |
| CTTTACAGGGACGAAATGGG+AGG | 0.647206 | 8:-4174278 | None:intergenic |
| CCTCAACCTGAATCCTCCCG+TGG | 0.682996 | 8:-4173664 | MsG0880042131.01.T01:CDS |
| ACAGGTGGAAGATTTCACGC+CGG | 0.710438 | 8:-4173900 | MsG0880042131.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTTGCTTTATATATTAAA+GGG | + | Chr8:4173660-4173679 | None:intergenic | 15.0% |
| !! | TATCTTGCTTTATATATTAA+AGG | + | Chr8:4173661-4173680 | None:intergenic | 15.0% |
| ! | AGAGAAATAATTTCTCTTAG+TGG | + | Chr8:4173924-4173943 | None:intergenic | 25.0% |
| ! | ATCAAAGAGAAGAAAACATA+CGG | - | Chr8:4174258-4174277 | MsG0880042131.01.T01:intron | 25.0% |
| ! | TAACAATCCAATATCATCTA+AGG | + | Chr8:4173812-4173831 | None:intergenic | 25.0% |
| ! | TGTGTATAAATCCAATATCA+TGG | - | Chr8:4173686-4173705 | MsG0880042131.01.T01:CDS | 25.0% |
| !! | CTATAGAATTTTGCAATGTT+TGG | + | Chr8:4174036-4174055 | None:intergenic | 25.0% |
| AGACATAATGACAAGAAAAG+TGG | + | Chr8:4173856-4173875 | None:intergenic | 30.0% | |
| ATTGCAAAATTCTATAGCTG+CGG | - | Chr8:4174039-4174058 | MsG0880042131.01.T01:intron | 30.0% | |
| CAATAGAGATTCCATGATAT+TGG | + | Chr8:4173700-4173719 | None:intergenic | 30.0% | |
| TCAAATGGCCTATAATTACA+AGG | - | Chr8:4173617-4173636 | MsG0880042131.01.T01:CDS | 30.0% | |
| TCTTAGTGGTTTAACTCAAA+AGG | + | Chr8:4173910-4173929 | None:intergenic | 30.0% | |
| ! | CTTTTCGTCCTTGTAATTAT+AGG | + | Chr8:4173628-4173647 | None:intergenic | 30.0% |
| ! | GAAATTATTTCTCTTTCCAC+AGG | - | Chr8:4173930-4173949 | MsG0880042131.01.T01:intron | 30.0% |
| ! | GAATTTTGCAATGTTTGGTT+TGG | + | Chr8:4174031-4174050 | None:intergenic | 30.0% |
| ACAACTACTCCAAAAAGACA+TGG | - | Chr8:4174148-4174167 | MsG0880042131.01.T01:intron | 35.0% | |
| CAATCCAATATCATCTAAGG+AGG | + | Chr8:4173809-4173828 | None:intergenic | 35.0% | |
| GTATCTTCTGATCATTCTGA+TGG | - | Chr8:4174229-4174248 | MsG0880042131.01.T01:intron | 35.0% | |
| TCCAAACAGTGTGAAAAACA+TGG | + | Chr8:4173771-4173790 | None:intergenic | 35.0% | |
| ! | ATTATTTCTCTTTCCACAGG+TGG | - | Chr8:4173933-4173952 | MsG0880042131.01.T01:intron | 35.0% |
| ! | TATTGCTCCTTTGTTGTATC+TGG | - | Chr8:4173830-4173849 | MsG0880042131.01.T01:CDS | 35.0% |
| ! | TCACACTGTTTGGAATCATA+TGG | - | Chr8:4173777-4173796 | MsG0880042131.01.T01:CDS | 35.0% |
| !!! | CTAATTTTTTCGAGTGCTCT+AGG | + | Chr8:4173998-4174017 | None:intergenic | 35.0% |
| AAAGTGGCCAGATACAACAA+AGG | + | Chr8:4173840-4173859 | None:intergenic | 40.0% | |
| CTCAGGGACGAAAATTCAAA+TGG | - | Chr8:4173602-4173621 | MsG0880042131.01.T01:CDS | 40.0% | |
| CTGACCTCCTTAGATGATAT+TGG | - | Chr8:4173802-4173821 | MsG0880042131.01.T01:CDS | 40.0% | |
| TCTTCTGATCATTCTGATGG+AGG | - | Chr8:4174232-4174251 | MsG0880042131.01.T01:intron | 40.0% | |
| ! | CTGTTCTCACCATGTCTTTT+TGG | + | Chr8:4174160-4174179 | None:intergenic | 40.0% |
| ! | GCCATGTTTTTCACACTGTT+TGG | - | Chr8:4173767-4173786 | MsG0880042131.01.T01:CDS | 40.0% |
| AGAGCGAAGAGCTCAATATC+TGG | - | Chr8:4174112-4174131 | MsG0880042131.01.T01:intron | 45.0% | |
| ! | ATGGGAGGATTAGTAAGCTC+AGG | - | Chr8:4173585-4173604 | MsG0880042131.01.T01:CDS | 45.0% |
| ! | TGGGAGGATTAGTAAGCTCA+GGG | - | Chr8:4173586-4173605 | MsG0880042131.01.T01:CDS | 45.0% |
| !! | TTTCGAGTGCTCTAGGATCA+GGG | + | Chr8:4173991-4174010 | None:intergenic | 45.0% |
| !!! | TTTTCGAGTGCTCTAGGATC+AGG | + | Chr8:4173992-4174011 | None:intergenic | 45.0% |
| ACAGGTGGAAGATTTCACGC+CGG | - | Chr8:4173948-4173967 | MsG0880042131.01.T01:intron | 50.0% | |
| GAAGATTTCACGCCGGGTTT+CGG | - | Chr8:4173955-4173974 | MsG0880042131.01.T01:intron | 50.0% | |
| !! | GGTAATGGACAACCGAAACC+CGG | + | Chr8:4173970-4173989 | None:intergenic | 50.0% |
| !! | GTGCTCTAGGATCAGGGTAA+TGG | + | Chr8:4173985-4174004 | None:intergenic | 50.0% |
| AAACGCCAGAGCTGTGACCA+CGG | + | Chr8:4174204-4174223 | None:intergenic | 55.0% | |
| CAGGTGGAAGATTTCACGCC+GGG | - | Chr8:4173949-4173968 | MsG0880042131.01.T01:intron | 55.0% | |
| GGCGTGAAATCTTCCACCTG+TGG | + | Chr8:4173949-4173968 | None:intergenic | 55.0% | |
| AACGCCAGAGCTGTGACCAC+GGG | + | Chr8:4174203-4174222 | None:intergenic | 60.0% | |
| CCTCAACCTGAATCCTCCCG+TGG | - | Chr8:4174184-4174203 | MsG0880042131.01.T01:intron | 60.0% | |
| CTGTGACCACGGGAGGATTC+AGG | + | Chr8:4174193-4174212 | None:intergenic | 60.0% | |
| !! | CCACGGGAGGATTCAGGTTG+AGG | + | Chr8:4174187-4174206 | None:intergenic | 60.0% |
| TCCTCCCGTGGTCACAGCTC+TGG | - | Chr8:4174196-4174215 | MsG0880042131.01.T01:intron | 65.0% | |
| GCCAGAGCTGTGACCACGGG+AGG | + | Chr8:4174200-4174219 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 4173585 | 4174285 | 4173585 | ID=MsG0880042131.01;Name=MsG0880042131.01 |
| Chr8 | mRNA | 4173585 | 4174285 | 4173585 | ID=MsG0880042131.01.T01;Parent=MsG0880042131.01;Name=MsG0880042131.01.T01;_AED=0.50;_eAED=0.55;_QI=0|0|0|1|0|0|2|0|122 |
| Chr8 | exon | 4174274 | 4174285 | 4174274 | ID=MsG0880042131.01.T01:exon:2505;Parent=MsG0880042131.01.T01 |
| Chr8 | exon | 4173585 | 4173941 | 4173585 | ID=MsG0880042131.01.T01:exon:2504;Parent=MsG0880042131.01.T01 |
| Chr8 | CDS | 4174274 | 4174285 | 4174274 | ID=MsG0880042131.01.T01:cds;Parent=MsG0880042131.01.T01 |
| Chr8 | CDS | 4173585 | 4173941 | 4173585 | ID=MsG0880042131.01.T01:cds;Parent=MsG0880042131.01.T01 |
| Gene Sequence |
| Protein sequence |
