AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025242.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATTGTTGAAGCCACT+TGG 0.460840 5:+15778796 MsG0580025242.01.T01:CDS
TCAAGATGTTGAGGTATTCA+AGG 0.462945 5:+15778683 MsG0580025242.01.T01:CDS
TTGAATCAACCTGGATTCCT+CGG 0.465616 5:-15778664 None:intergenic
AGGTATTCAAGGGCATTGAT+TGG 0.489346 5:+15778694 MsG0580025242.01.T01:CDS
CTCAACATCTTGAATCAACC+TGG 0.515554 5:-15778673 None:intergenic
ATGCAACTCTGCTGGTGTAA+TGG 0.527579 5:+15778761 MsG0580025242.01.T01:CDS
TGAATCAACCTGGATTCCTC+GGG 0.539173 5:-15778663 None:intergenic
TGCAAAGCATGCAACTCTGC+TGG 0.554272 5:+15778753 MsG0580025242.01.T01:CDS
AACTCTGCTGGTGTAATGGC+TGG 0.557213 5:+15778765 MsG0580025242.01.T01:CDS
CTGATATGGTGAATGGCTGG+TGG 0.575310 5:+15778603 None:intergenic
TTTCTGATATGGTGAATGGC+TGG 0.577825 5:+15778600 None:intergenic
CAAGATGTTGAGGTATTCAA+GGG 0.581251 5:+15778684 MsG0580025242.01.T01:CDS
GAGTTGCATGCTTTGCACCA+TGG 0.594770 5:-15778747 None:intergenic
CAGGTTGATTCAAGATGTTG+AGG 0.617694 5:+15778674 MsG0580025242.01.T01:CDS
AAATTGTTGAAGCCACTTGG+TGG 0.620286 5:+15778799 MsG0580025242.01.T01:CDS
CTGTTGAAGATCGCATTCCA+TGG 0.631541 5:+15778730 MsG0580025242.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCAAATTGTTGAAGCCACT+TGG + Chr5:15778796-15778815 MsG0580025242.01.T01:CDS 35.0%
! CAAGATGTTGAGGTATTCAA+GGG + Chr5:15778684-15778703 MsG0580025242.01.T01:CDS 35.0%
! TCAAGATGTTGAGGTATTCA+AGG + Chr5:15778683-15778702 MsG0580025242.01.T01:CDS 35.0%
AAAAACAATGCAGCAGCAAG+TGG - Chr5:15778643-15778662 None:intergenic 40.0%
AAATTGTTGAAGCCACTTGG+TGG + Chr5:15778799-15778818 MsG0580025242.01.T01:CDS 40.0%
CTCAACATCTTGAATCAACC+TGG - Chr5:15778676-15778695 None:intergenic 40.0%
TTGAATCAACCTGGATTCCT+CGG - Chr5:15778667-15778686 None:intergenic 40.0%
!! AGGTATTCAAGGGCATTGAT+TGG + Chr5:15778694-15778713 MsG0580025242.01.T01:CDS 40.0%
!! CAGGTTGATTCAAGATGTTG+AGG + Chr5:15778674-15778693 MsG0580025242.01.T01:CDS 40.0%
ATGCAACTCTGCTGGTGTAA+TGG + Chr5:15778761-15778780 MsG0580025242.01.T01:CDS 45.0%
CTGTTGAAGATCGCATTCCA+TGG + Chr5:15778730-15778749 MsG0580025242.01.T01:CDS 45.0%
TGAATCAACCTGGATTCCTC+GGG - Chr5:15778666-15778685 None:intergenic 45.0%
!! TTGTTTTTCCCGAGGAATCC+AGG + Chr5:15778655-15778674 MsG0580025242.01.T01:CDS 45.0%
AACTCTGCTGGTGTAATGGC+TGG + Chr5:15778765-15778784 MsG0580025242.01.T01:CDS 50.0%
GAGTTGCATGCTTTGCACCA+TGG - Chr5:15778750-15778769 None:intergenic 50.0%
TGCAAAGCATGCAACTCTGC+TGG + Chr5:15778753-15778772 MsG0580025242.01.T01:CDS 50.0%
!! CTGCTGCATTGTTTTTCCCG+AGG + Chr5:15778647-15778666 MsG0580025242.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 15778615 15778830 15778615 ID=MsG0580025242.01;Name=MsG0580025242.01
Chr5 mRNA 15778615 15778830 15778615 ID=MsG0580025242.01.T01;Parent=MsG0580025242.01;Name=MsG0580025242.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|71
Chr5 exon 15778615 15778830 15778615 ID=MsG0580025242.01.T01:exon:30173;Parent=MsG0580025242.01.T01
Chr5 CDS 15778615 15778830 15778615 ID=MsG0580025242.01.T01:cds;Parent=MsG0580025242.01.T01
Gene Sequence

>MsG0580025242.01.T01

ATGGCTGGTGGTTTTTCGTACACGACCACTTGCTGCTGCATTGTTTTTCCCGAGGAATCCAGGTTGATTCAAGATGTTGAGGTATTCAAGGGCATTGATTGGTTCTTTGCTAATGCTGTTGAAGATCGCATTCCATGGTGCAAAGCATGCAACTCTGCTGGTGTAATGGCTGGAAGACAACATCAAATTGTTGAAGCCACTTGGTGGTGTGACTAA

Protein sequence

>MsG0580025242.01.T01

MAGGFSYTTTCCCIVFPEESRLIQDVEVFKGIDWFFANAVEDRIPWCKACNSAGVMAGRQHQIVEATWWCD*