AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047427.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047427.01.T01 MTR_4g127160 98.113 53 1 0 4 56 255 307 3.28e-32 113
MsG0880047427.01.T01 MTR_4g127160 98.113 53 1 0 4 56 324 376 1.40e-31 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047427.01.T01 AT5G57120 50.909 55 24 1 2 56 244 295 4.99e-11 56.2

Find 29 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAACAATATTGTTCATT+TGG 0.312566 8:-86132509 MsG0880047427.01.T01:three_prime_UTR
TATAGGTCCCACGTTTCTTC+TGG 0.318897 8:+86132702 None:intergenic
ATTTGTTGATGAGAGGCTTC+AGG 0.356972 8:-86133321 MsG0880047427.01.T01:CDS
GAAGAAACGTGGGACCTATA+GGG 0.376538 8:-86132699 MsG0880047427.01.T01:three_prime_UTR
GGCTTCAGGATAACTCATAT+TGG 0.387596 8:-86133307 MsG0880047427.01.T01:CDS
AGAAGAAACGTGGGACCTAT+AGG 0.406965 8:-86132700 MsG0880047427.01.T01:three_prime_UTR
GATGGCGCCGAGAGTGGTTA+TGG 0.413963 8:-86133192 MsG0880047427.01.T01:CDS
CGTTGAAATGCTGCCTTCAC+TGG 0.414815 8:+86133365 None:intergenic
GCAAAAGCAGAAGAAATTCT+TGG 0.442256 8:-86133168 MsG0880047427.01.T01:CDS
GCTTCAGGATAACTCATATT+GGG 0.455100 8:-86133306 MsG0880047427.01.T01:CDS
ATCAATAAGTCCACCCCTAT+AGG 0.468521 8:+86132685 None:intergenic
TTATTCAGATGAGGAGTGAC+TGG 0.475043 8:-86132636 MsG0880047427.01.T01:three_prime_UTR
CTGCAGGATGGCGCCGAGAG+TGG 0.518486 8:-86133198 MsG0880047427.01.T01:intron
AATAATTAAACTTAACTGAG+TGG 0.520249 8:+86132654 None:intergenic
CGTTTCTTCTGGTCTTCTCG+TGG 0.543171 8:+86132713 None:intergenic
GAAATTCTTGGTCAAGTCAG+AGG 0.547509 8:-86133156 MsG0880047427.01.T01:CDS
TAAGTTTAATTATTCAGATG+AGG 0.552944 8:-86132645 MsG0880047427.01.T01:three_prime_UTR
AAGTAGAATTTGTTGATGAG+AGG 0.558430 8:-86133328 MsG0880047427.01.T01:CDS
ATAATTAAACTTAACTGAGT+GGG 0.560601 8:+86132655 None:intergenic
AAGAAACGTGGGACCTATAG+GGG 0.566821 8:-86132698 MsG0880047427.01.T01:three_prime_UTR
TGATAACACCGGCTTTAGCA+TGG 0.570584 8:+86132555 None:intergenic
CTTGAGCACCATGCTAAAGC+CGG 0.571283 8:-86132563 MsG0880047427.01.T01:three_prime_UTR
CGAGAAGACCAGAAGAAACG+TGG 0.589630 8:-86132710 MsG0880047427.01.T01:three_prime_UTR
GGATAACTCATATTGGGCAA+AGG 0.615077 8:-86133300 MsG0880047427.01.T01:intron
TTCTTGGTCAAGTCAGAGGA+AGG 0.619626 8:-86133152 MsG0880047427.01.T01:CDS
TATTCAGATGAGGAGTGACT+GGG 0.640238 8:-86132635 MsG0880047427.01.T01:three_prime_UTR
GAGAAGACCAGAAGAAACGT+GGG 0.649752 8:-86132709 MsG0880047427.01.T01:three_prime_UTR
AAACGTGGGACCTATAGGGG+TGG 0.658830 8:-86132695 MsG0880047427.01.T01:three_prime_UTR
TCAGATGAGGAGTGACTGGG+AGG 0.685329 8:-86132632 MsG0880047427.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CACATTATAAAACATTATAT+TGG + Chr8:86132956-86132975 None:intergenic 15.0%
!!! ATCAAAATTTTGTTACATTA+TGG - Chr8:86133236-86133255 MsG0880047427.01.T01:intron 15.0%
!! AATAATTAAACTTAACTGAG+TGG + Chr8:86133121-86133140 None:intergenic 20.0%
!! ACCAAAAAAGTAAAATAAGA+TGG + Chr8:86133007-86133026 None:intergenic 20.0%
!! ATAATTAAACTTAACTGAGT+GGG + Chr8:86133120-86133139 None:intergenic 20.0%
!! ATTATCTAAGAAATGTGTAT+GGG - Chr8:86132542-86132561 MsG0880047427.01.T01:three_prime_UTR 20.0%
!! GAAATAAAATTGTTTCACTA+CGG + Chr8:86132661-86132680 None:intergenic 20.0%
!! TAAGTTTAATTATTCAGATG+AGG - Chr8:86133127-86133146 MsG0880047427.01.T01:intron 20.0%
!! TTGAAACAATATTGTTCATT+TGG - Chr8:86133263-86133282 MsG0880047427.01.T01:intron 20.0%
!! TTTAATACAAGTGCAAATAT+TGG + Chr8:86132683-86132702 None:intergenic 20.0%
!!! ACCATCTTATTTTACTTTTT+TGG - Chr8:86133003-86133022 MsG0880047427.01.T01:intron 20.0%
!!! TAACAAAATTTTGATAACAC+CGG + Chr8:86133231-86133250 None:intergenic 20.0%
! ATTTCTTAGATAATGCAACT+GGG + Chr8:86132536-86132555 None:intergenic 25.0%
! CATTATCTAAGAAATGTGTA+TGG - Chr8:86132541-86132560 MsG0880047427.01.T01:three_prime_UTR 25.0%
! GATATATGTGTGAAAAATCT+GGG + Chr8:86132884-86132903 None:intergenic 25.0%
! GGTTTAGAAAAATGAATGAA+AGG + Chr8:86132911-86132930 None:intergenic 25.0%
! TAGCTGAATAAGACAATAAA+GGG + Chr8:86132932-86132951 None:intergenic 25.0%
! TTAGCTGAATAAGACAATAA+AGG + Chr8:86132933-86132952 None:intergenic 25.0%
! TTCATAGACAAGCTATAAAT+AGG - Chr8:86132765-86132784 MsG0880047427.01.T01:intron 25.0%
!! CAGATTTTTCACACATATAT+CGG - Chr8:86132883-86132902 MsG0880047427.01.T01:intron 25.0%
!! CTATGGAAAATTTGTTTGAT+GGG - Chr8:86132833-86132852 MsG0880047427.01.T01:intron 25.0%
!! TCTATGGAAAATTTGTTTGA+TGG - Chr8:86132832-86132851 MsG0880047427.01.T01:intron 25.0%
!!! TGTTTTGATTGCATATCAAA+TGG - Chr8:86133369-86133388 MsG0880047427.01.T01:CDS 25.0%
ACACCAAATATTTCTCTTAC+AGG - Chr8:86133028-86133047 MsG0880047427.01.T01:intron 30.0%
ATCCCTGTAAGAGAAATATT+TGG + Chr8:86133034-86133053 None:intergenic 30.0%
CACCAAATATTTCTCTTACA+GGG - Chr8:86133029-86133048 MsG0880047427.01.T01:intron 30.0%
CATTTCTTAGATAATGCAAC+TGG + Chr8:86132537-86132556 None:intergenic 30.0%
CGATATATGTGTGAAAAATC+TGG + Chr8:86132885-86132904 None:intergenic 30.0%
! CAAAATTTTGCTACATTACG+AGG - Chr8:86133336-86133355 MsG0880047427.01.T01:CDS 30.0%
! TGATATGTTTTCCATGCATT+TGG - Chr8:86132505-86132524 MsG0880047427.01.T01:three_prime_UTR 30.0%
! TTTGTCTAGTGAGTTTTCTA+TGG - Chr8:86132816-86132835 MsG0880047427.01.T01:intron 30.0%
!! AAGTAGAATTTGTTGATGAG+AGG - Chr8:86132444-86132463 MsG0880047427.01.T01:three_prime_UTR 30.0%
CTATAAATAGGTCATGCTGT+TGG - Chr8:86132777-86132796 MsG0880047427.01.T01:intron 35.0%
GCAAAAGCAGAAGAAATTCT+TGG - Chr8:86132604-86132623 MsG0880047427.01.T01:three_prime_UTR 35.0%
GCTTCAGGATAACTCATATT+GGG - Chr8:86132466-86132485 MsG0880047427.01.T01:three_prime_UTR 35.0%
ATCAATAAGTCCACCCCTAT+AGG + Chr8:86133090-86133109 None:intergenic 40.0%
ATTGCAGCAAAAACCAGTGA+AGG - Chr8:86132394-86132413 MsG0880047427.01.T01:three_prime_UTR 40.0%
GAAATTCTTGGTCAAGTCAG+AGG - Chr8:86132616-86132635 MsG0880047427.01.T01:three_prime_UTR 40.0%
GGATAACTCATATTGGGCAA+AGG - Chr8:86132472-86132491 MsG0880047427.01.T01:three_prime_UTR 40.0%
GGCTTCAGGATAACTCATAT+TGG - Chr8:86132465-86132484 MsG0880047427.01.T01:three_prime_UTR 40.0%
TATTCAGATGAGGAGTGACT+GGG - Chr8:86133137-86133156 MsG0880047427.01.T01:CDS 40.0%
TTATTCAGATGAGGAGTGAC+TGG - Chr8:86133136-86133155 MsG0880047427.01.T01:CDS 40.0%
! ATTTGTTGATGAGAGGCTTC+AGG - Chr8:86132451-86132470 MsG0880047427.01.T01:three_prime_UTR 40.0%
! TTTTGCACCATAACCACTCT+CGG + Chr8:86132590-86132609 None:intergenic 40.0%
AAGAAACGTGGGACCTATAG+GGG - Chr8:86133074-86133093 MsG0880047427.01.T01:intron 45.0%
AGAAGAAACGTGGGACCTAT+AGG - Chr8:86133072-86133091 MsG0880047427.01.T01:intron 45.0%
GAAGAAACGTGGGACCTATA+GGG - Chr8:86133073-86133092 MsG0880047427.01.T01:intron 45.0%
GAGAAGACCAGAAGAAACGT+GGG - Chr8:86133063-86133082 MsG0880047427.01.T01:intron 45.0%
TATAGGTCCCACGTTTCTTC+TGG + Chr8:86133073-86133092 None:intergenic 45.0%
TTCTTGGTCAAGTCAGAGGA+AGG - Chr8:86132620-86132639 MsG0880047427.01.T01:three_prime_UTR 45.0%
!! TGATAACACCGGCTTTAGCA+TGG + Chr8:86133220-86133239 None:intergenic 45.0%
ACTGGGACACACCAAATGCA+TGG + Chr8:86132519-86132538 None:intergenic 50.0%
CGAGAAGACCAGAAGAAACG+TGG - Chr8:86133062-86133081 MsG0880047427.01.T01:intron 50.0%
CGTTGAAATGCTGCCTTCAC+TGG + Chr8:86132410-86132429 None:intergenic 50.0%
CTTGAGCACCATGCTAAAGC+CGG - Chr8:86133209-86133228 MsG0880047427.01.T01:intron 50.0%
GTATGGGTCTATCTCTCTGC+AGG - Chr8:86132558-86132577 MsG0880047427.01.T01:three_prime_UTR 50.0%
! CGTTTCTTCTGGTCTTCTCG+TGG + Chr8:86133062-86133081 None:intergenic 50.0%
AAACGTGGGACCTATAGGGG+TGG - Chr8:86133077-86133096 MsG0880047427.01.T01:intron 55.0%
GGGTCTATCTCTCTGCAGGA+TGG - Chr8:86132562-86132581 MsG0880047427.01.T01:three_prime_UTR 55.0%
TCAGATGAGGAGTGACTGGG+AGG - Chr8:86133140-86133159 MsG0880047427.01.T01:CDS 55.0%
! GATGGCGCCGAGAGTGGTTA+TGG - Chr8:86132580-86132599 MsG0880047427.01.T01:three_prime_UTR 60.0%
! CTGCAGGATGGCGCCGAGAG+TGG - Chr8:86132574-86132593 MsG0880047427.01.T01:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86132389 86133405 86132389 ID=MsG0880047427.01;Name=MsG0880047427.01
Chr8 mRNA 86132389 86133405 86132389 ID=MsG0880047427.01.T01;Parent=MsG0880047427.01;Name=MsG0880047427.01.T01;_AED=0.46;_eAED=0.49;_QI=0|0.5|0.33|1|0.5|0.33|3|355|62
Chr8 exon 86132389 86132744 86132389 ID=MsG0880047427.01.T01:exon:2035;Parent=MsG0880047427.01.T01
Chr8 exon 86133132 86133214 86133132 ID=MsG0880047427.01.T01:exon:2034;Parent=MsG0880047427.01.T01
Chr8 exon 86133301 86133405 86133301 ID=MsG0880047427.01.T01:exon:2033;Parent=MsG0880047427.01.T01
Chr8 CDS 86133301 86133405 86133301 ID=MsG0880047427.01.T01:cds;Parent=MsG0880047427.01.T01
Chr8 CDS 86133132 86133214 86133132 ID=MsG0880047427.01.T01:cds;Parent=MsG0880047427.01.T01
Chr8 CDS 86132744 86132744 86132744 ID=MsG0880047427.01.T01:cds;Parent=MsG0880047427.01.T01
Chr8 three_prime_UTR 86132389 86132743 86132389 ID=MsG0880047427.01.T01:three_prime_utr;Parent=MsG0880047427.01.T01
Gene Sequence

>MsG0880047427.01.T01

ATGATATTGCAGCAAAAACCAGTGAAGGCAGCATTTCAACGCGTACAAGTTGACAAAGTAGAATTTGTTGATGAGAGGCTTCAGGATAACTCATATTGGGCAAAGGATGGCGCCGAGAGTGGTTATGGTGCAAAAGCAGAAGAAATTCTTGGTCAAGTCAGAGGAAGGTCTGTTATTTCATATCCGTAG

Protein sequence

>MsG0880047427.01.T01

MILQQKPVKAAFQRVQVDKVEFVDERLQDNSYWAKDGAESGYGAKAEEILGQVRGRSVISYP*