AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029549.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029549.01.T01 MTR_5g088340 100.000 100 0 0 29 128 283 382 3.03e-63 197
MsG0580029549.01.T01 MTR_3g040090 76.000 100 24 0 29 128 276 375 4.48e-46 153
MsG0580029549.01.T01 MTR_3g040090 74.747 99 25 0 29 127 276 374 2.71e-44 150
MsG0580029549.01.T01 MTR_3g040090 75.000 40 10 0 29 68 276 315 1.57e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029549.01.T01 AT3G20320 74.000 100 26 0 29 128 282 381 5.27e-46 153

Find 43 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTTCTTAAGTGTTTAAT+TGG 0.167215 5:-98430767 MsG0580029549.01.T01:three_prime_UTR
TTGGTTTCTGCTCAAGTTAT+TGG 0.216205 5:-98430863 MsG0580029549.01.T01:three_prime_UTR
AAAATGAAGTCACAGTTTAA+AGG 0.271407 5:-98430796 MsG0580029549.01.T01:three_prime_UTR
TAAGTGTGTGTATGGACTTC+TGG 0.303339 5:+98431578 None:intergenic
AATTTCTTAAGTGTTTAATT+GGG 0.340276 5:-98430766 MsG0580029549.01.T01:three_prime_UTR
CCCTAGCTTCTGATGATTTG+AGG 0.341669 5:-98432178 MsG0580029549.01.T01:intron
TCTAGATGTTCAACCTTTGT+TGG 0.383759 5:-98432258 MsG0580029549.01.T01:CDS
GGTAAATATAAGTGTGTGTA+TGG 0.394496 5:+98431570 None:intergenic
GCAGAATATTACCTCTGATA+TGG 0.395381 5:-98431452 MsG0580029549.01.T01:intron
CAGAAGCTAGGGTAAGGCTT+CGG 0.397566 5:+98432189 None:intergenic
CCTGTTATATGCTACTTCAC+TGG 0.413187 5:+98431345 None:intergenic
ACTACATCAAAACGAAAATA+CGG 0.415713 5:+98432305 None:intergenic
CCAGTGAAGTAGCATATAAC+AGG 0.418405 5:-98431345 MsG0580029549.01.T01:intron
CAATTAATACATTGCTAAGA+TGG 0.426922 5:+98430718 None:intergenic
TCAAACCTCAATGTTCTTCA+AGG 0.432770 5:+98431549 None:intergenic
AGAAGCTAGGGTAAGGCTTC+GGG 0.447061 5:+98432190 None:intergenic
AACTCTCAACTTCCTTCAAT+AGG 0.459217 5:+98432216 None:intergenic
CATTAAACTACACTGTTGCA+TGG 0.474864 5:+98430673 None:intergenic
CCGTGATAGCGGCCTATTGA+AGG 0.476864 5:-98432228 MsG0580029549.01.T01:CDS
CAAGTTATTGGCGCCATCTG+TGG 0.490797 5:-98430851 MsG0580029549.01.T01:three_prime_UTR
GATCAGCTCAGCGTTCTCAT+TGG 0.514219 5:+98431600 None:intergenic
TGTTCAACCTTTGTTGGCCG+AGG 0.515090 5:-98432252 MsG0580029549.01.T01:CDS
ACCTCAAATCATCAGAAGCT+AGG 0.518321 5:+98432177 None:intergenic
CCTCAAATCATCAGAAGCTA+GGG 0.532646 5:+98432178 None:intergenic
GAGAATGCATCATATAGTAA+AGG 0.535409 5:+98432332 None:intergenic
ATTTCTTAAGTGTTTAATTG+GGG 0.542314 5:-98430765 MsG0580029549.01.T01:three_prime_UTR
AATCATCAGAAGCTAGGGTA+AGG 0.550341 5:+98432183 None:intergenic
TACTTATCAAGAATCTCAGC+AGG 0.558675 5:-98431387 MsG0580029549.01.T01:CDS
TTGGCCGAGGTCCGTGATAG+CGG 0.559975 5:-98432239 MsG0580029549.01.T01:CDS
ATTAAACTACACTGTTGCAT+GGG 0.566235 5:+98430674 None:intergenic
AATATTACCTCTGATATGGT+AGG 0.567654 5:-98431448 MsG0580029549.01.T01:CDS
CTACATCAAAACGAAAATAC+GGG 0.571708 5:+98432306 None:intergenic
ATTTACCTTGAAGAACATTG+AGG 0.581288 5:-98431554 MsG0580029549.01.T01:intron
AGAGCCATGGCTTTCATCTG+AGG 0.583663 5:+98432278 None:intergenic
GTGTAGTTTAATGTTACAGA+GGG 0.585646 5:-98430663 MsG0580029549.01.T01:three_prime_UTR
CCTTCAATAGGCCGCTATCA+CGG 0.604578 5:+98432228 None:intergenic
TCACGGACCTCGGCCAACAA+AGG 0.611021 5:+98432245 None:intergenic
TAGGCCGCTATCACGGACCT+CGG 0.624041 5:+98432235 None:intergenic
AGGTTGAACATCTAGAGCCA+TGG 0.633693 5:+98432265 None:intergenic
CAAGTGACATGATCCACAGA+TGG 0.638519 5:+98430838 None:intergenic
AGTGTAGTTTAATGTTACAG+AGG 0.647387 5:-98430664 MsG0580029549.01.T01:three_prime_UTR
AGTAAAACCTACCATATCAG+AGG 0.647833 5:+98431441 None:intergenic
GATGCCTCAGATGAAAGCCA+TGG 0.676272 5:-98432282 MsG0580029549.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTAATTTAAATTGTTTTAA+GGG - Chr5:98431674-98431693 MsG0580029549.01.T01:intron 10.0%
!!! TAGTAATTTAAATTGTTTTA+AGG - Chr5:98431673-98431692 MsG0580029549.01.T01:intron 10.0%
!! AATTTCTTAAGTGTTTAATT+GGG - Chr5:98432177-98432196 MsG0580029549.01.T01:intron 15.0%
!! AATAAGAAAACACCAATTTA+TGG + Chr5:98431818-98431837 None:intergenic 20.0%
!! ATTTCTTAAGTGTTTAATTG+GGG - Chr5:98432178-98432197 MsG0580029549.01.T01:intron 20.0%
!! CATATTAGGATAAACATAAT+AGG + Chr5:98431164-98431183 None:intergenic 20.0%
!! GAATTTCTTAAGTGTTTAAT+TGG - Chr5:98432176-98432195 MsG0580029549.01.T01:intron 20.0%
!!! AAGTAGTTTCAAATTCTTTT+TGG + Chr5:98431541-98431560 None:intergenic 20.0%
!!! AATACACAGAATTTTTGAAT+TGG + Chr5:98431205-98431224 None:intergenic 20.0%
!!! ACTTTTTCTCATGTTTTTTT+TGG - Chr5:98431458-98431477 MsG0580029549.01.T01:intron 20.0%
!!! TTTAGCTTATTTCCATAAAT+TGG - Chr5:98431803-98431822 MsG0580029549.01.T01:intron 20.0%
! AAAATGAAGTCACAGTTTAA+AGG - Chr5:98432147-98432166 MsG0580029549.01.T01:intron 25.0%
! AAACTATCATGGAAAACATA+TGG + Chr5:98431842-98431861 None:intergenic 25.0%
! AAATAAGCTAAACCAACTTA+TGG + Chr5:98431795-98431814 None:intergenic 25.0%
! AATAGCTTATACATAAGAAC+AGG - Chr5:98431899-98431918 MsG0580029549.01.T01:intron 25.0%
! ACTACATCAAAACGAAAATA+CGG + Chr5:98430641-98430660 None:intergenic 25.0%
! AGATTTGTCAGAGATTTAAT+GGG - Chr5:98430933-98430952 MsG0580029549.01.T01:intron 25.0%
! CAATCATAATTTATACCAAG+AGG + Chr5:98430910-98430929 None:intergenic 25.0%
! CAATTAATACATTGCTAAGA+TGG + Chr5:98432228-98432247 None:intergenic 25.0%
! CTTGGTATAAATTATGATTG+AGG - Chr5:98430910-98430929 MsG0580029549.01.T01:three_prime_UTR 25.0%
! GATTTAATGGGAAAAATATG+AGG - Chr5:98430945-98430964 MsG0580029549.01.T01:intron 25.0%
! TCCATGATAGTTTATGAAAA+CGG - Chr5:98431849-98431868 MsG0580029549.01.T01:intron 25.0%
! TGTGCTTATCAATATATGAA+TGG + Chr5:98431025-98431044 None:intergenic 25.0%
!! CATGTGAAATTTCAGTTTTA+GGG + Chr5:98431097-98431116 None:intergenic 25.0%
!! TAATAATTTTTCTCATGCAG+TGG + Chr5:98431140-98431159 None:intergenic 25.0%
!! TCATGTGAAATTTCAGTTTT+AGG + Chr5:98431098-98431117 None:intergenic 25.0%
!! TTTTTACGTGACAACTTTTT+TGG - Chr5:98432061-98432080 MsG0580029549.01.T01:intron 25.0%
!!! ACTTCATTTTTCTCAAAATC+TGG + Chr5:98432137-98432156 None:intergenic 25.0%
!!! GGCTTTCAATACTTTTTTTA+AGG - Chr5:98432301-98432320 MsG0580029549.01.T01:CDS 25.0%
!!! TTCAATACTTTTTTTAAGGC+AGG - Chr5:98432305-98432324 MsG0580029549.01.T01:CDS 25.0%
AATATTACCTCTGATATGGT+AGG - Chr5:98431495-98431514 MsG0580029549.01.T01:intron 30.0%
AGTGTAGTTTAATGTTACAG+AGG - Chr5:98432279-98432298 MsG0580029549.01.T01:CDS 30.0%
ATTAAACTACACTGTTGCAT+GGG + Chr5:98432272-98432291 None:intergenic 30.0%
ATTCTCACAAGTTGCATATT+AGG + Chr5:98431178-98431197 None:intergenic 30.0%
ATTTACCTTGAAGAACATTG+AGG - Chr5:98431389-98431408 MsG0580029549.01.T01:CDS 30.0%
ATTTATTTGTGCTTATCTGC+TGG - Chr5:98431714-98431733 MsG0580029549.01.T01:intron 30.0%
CTACATCAAAACGAAAATAC+GGG + Chr5:98430640-98430659 None:intergenic 30.0%
CTTATGACATATCCATAAGT+TGG - Chr5:98431780-98431799 MsG0580029549.01.T01:intron 30.0%
GAGAATGCATCATATAGTAA+AGG + Chr5:98430614-98430633 None:intergenic 30.0%
GAGATTTGTCAGAGATTTAA+TGG - Chr5:98430932-98430951 MsG0580029549.01.T01:intron 30.0%
GCAGATAAGCACAAATAAAT+AGG + Chr5:98431714-98431733 None:intergenic 30.0%
GGTAAATATAAGTGTGTGTA+TGG + Chr5:98431376-98431395 None:intergenic 30.0%
GTGTAGTTTAATGTTACAGA+GGG - Chr5:98432280-98432299 MsG0580029549.01.T01:CDS 30.0%
TACATGTATGCAATTATGCA+TGG + Chr5:98430838-98430857 None:intergenic 30.0%
TCAATTAGTTACCATGTTTC+AGG + Chr5:98431278-98431297 None:intergenic 30.0%
! AAGCACTTGAGAAATTGTTT+TGG - Chr5:98431740-98431759 MsG0580029549.01.T01:intron 30.0%
! ACCGTTTTCATAAACTATCA+TGG + Chr5:98431853-98431872 None:intergenic 30.0%
! ATGGCAACTGATTTAAACTT+AGG + Chr5:98431006-98431025 None:intergenic 30.0%
! TATTTTCTCCTTCAATGTTG+TGG + Chr5:98431655-98431674 None:intergenic 30.0%
!!! AAATTGTTTTGGAGAGCTTA+CGG - Chr5:98431751-98431770 MsG0580029549.01.T01:intron 30.0%
AACTCTCAACTTCCTTCAAT+AGG + Chr5:98430730-98430749 None:intergenic 35.0%
AATTAAGCTGTTCATCCAAC+AGG - Chr5:98431942-98431961 MsG0580029549.01.T01:intron 35.0%
AATTCAACTCTCCTGAAACA+TGG - Chr5:98431264-98431283 MsG0580029549.01.T01:intron 35.0%
AGTAAAACCTACCATATCAG+AGG + Chr5:98431505-98431524 None:intergenic 35.0%
ATTAAGCTGTTCATCCAACA+GGG - Chr5:98431943-98431962 MsG0580029549.01.T01:intron 35.0%
ATTCCCTTTGTGTATTCAGA+AGG - Chr5:98431301-98431320 MsG0580029549.01.T01:intron 35.0%
CATTAAACTACACTGTTGCA+TGG + Chr5:98432273-98432292 None:intergenic 35.0%
CGCACAAATCTTATCACATA+AGG + Chr5:98431051-98431070 None:intergenic 35.0%
CTGATAAAAGTTGTCTTCTG+TGG - Chr5:98431997-98432016 MsG0580029549.01.T01:intron 35.0%
CTGATTTAAACTTAGGACGA+GGG + Chr5:98430999-98431018 None:intergenic 35.0%
GCAAGAAGAAAGATAGACTT+AGG + Chr5:98431969-98431988 None:intergenic 35.0%
GCAGAATATTACCTCTGATA+TGG - Chr5:98431491-98431510 MsG0580029549.01.T01:intron 35.0%
TACTTATCAAGAATCTCAGC+AGG - Chr5:98431556-98431575 MsG0580029549.01.T01:CDS 35.0%
TCAAACCTCAATGTTCTTCA+AGG + Chr5:98431397-98431416 None:intergenic 35.0%
TCTAGATGTTCAACCTTTGT+TGG - Chr5:98430685-98430704 MsG0580029549.01.T01:three_prime_UTR 35.0%
TGACAAACTCAATAACCTCT+TGG - Chr5:98430892-98430911 MsG0580029549.01.T01:three_prime_UTR 35.0%
TTCCCTTTGTGTATTCAGAA+GGG - Chr5:98431302-98431321 MsG0580029549.01.T01:intron 35.0%
TTGGTTTCTGCTCAAGTTAT+TGG - Chr5:98432080-98432099 MsG0580029549.01.T01:intron 35.0%
! ACTGATTTAAACTTAGGACG+AGG + Chr5:98431000-98431019 None:intergenic 35.0%
! TCTGATATGGTAGGTTTTAC+TGG - Chr5:98431504-98431523 MsG0580029549.01.T01:intron 35.0%
AATCATCAGAAGCTAGGGTA+AGG + Chr5:98430763-98430782 None:intergenic 40.0%
ACCTCAAATCATCAGAAGCT+AGG + Chr5:98430769-98430788 None:intergenic 40.0%
AGCACAATGTTGTTCGTGTA+GGG - Chr5:98430865-98430884 MsG0580029549.01.T01:three_prime_UTR 40.0%
CCAGTGAAGTAGCATATAAC+AGG - Chr5:98431598-98431617 MsG0580029549.01.T01:CDS 40.0%
CCTCAAATCATCAGAAGCTA+GGG + Chr5:98430768-98430787 None:intergenic 40.0%
GCTTCTCTGTACGACTAATT+TGG - Chr5:98430809-98430828 MsG0580029549.01.T01:three_prime_UTR 40.0%
GGGAAAAATATGAGGTTGTG+TGG - Chr5:98430953-98430972 MsG0580029549.01.T01:intron 40.0%
GTTGTGGCAGAGGAAAAATA+TGG + Chr5:98431639-98431658 None:intergenic 40.0%
TAAGTGTGTGTATGGACTTC+TGG + Chr5:98431368-98431387 None:intergenic 40.0%
TAGCACAATGTTGTTCGTGT+AGG - Chr5:98430864-98430883 MsG0580029549.01.T01:three_prime_UTR 40.0%
! CCTGTTATATGCTACTTCAC+TGG + Chr5:98431601-98431620 None:intergenic 40.0%
! TTTAGGGTCTTCAACTGAAC+AGG + Chr5:98431081-98431100 None:intergenic 40.0%
AAGATAGACTTAGGCCCTGT+TGG + Chr5:98431960-98431979 None:intergenic 45.0%
CAAGTGACATGATCCACAGA+TGG + Chr5:98432108-98432127 None:intergenic 45.0%
CCCTAGCTTCTGATGATTTG+AGG - Chr5:98430765-98430784 MsG0580029549.01.T01:three_prime_UTR 45.0%
CGCCCTTCTGAATACACAAA+GGG + Chr5:98431307-98431326 None:intergenic 45.0%
GCACAATGTTGTTCGTGTAG+GGG - Chr5:98430866-98430885 MsG0580029549.01.T01:three_prime_UTR 45.0%
GTCAGTTGCCTAAACACCAA+TGG - Chr5:98430787-98430806 MsG0580029549.01.T01:three_prime_UTR 45.0%
TAGTCGTACAGAGAAGCCAT+TGG + Chr5:98430806-98430825 None:intergenic 45.0%
TTCCTCTGCCACAACATTGA+AGG - Chr5:98431644-98431663 MsG0580029549.01.T01:intron 45.0%
! AGGTTGAACATCTAGAGCCA+TGG + Chr5:98430681-98430700 None:intergenic 45.0%
! CAGAGAAGCCATTGGTGTTT+AGG + Chr5:98430798-98430817 None:intergenic 45.0%
AGAAGCTAGGGTAAGGCTTC+GGG + Chr5:98430756-98430775 None:intergenic 50.0%
AGAGCCATGGCTTTCATCTG+AGG + Chr5:98430668-98430687 None:intergenic 50.0%
CAAGTTATTGGCGCCATCTG+TGG - Chr5:98432092-98432111 MsG0580029549.01.T01:intron 50.0%
CAGAAGCTAGGGTAAGGCTT+CGG + Chr5:98430757-98430776 None:intergenic 50.0%
CCTTCAATAGGCCGCTATCA+CGG + Chr5:98430718-98430737 None:intergenic 50.0%
CTCCTTCAATGTTGTGGCAG+AGG + Chr5:98431649-98431668 None:intergenic 50.0%
GATCAGCTCAGCGTTCTCAT+TGG + Chr5:98431346-98431365 None:intergenic 50.0%
GATGCCTCAGATGAAAGCCA+TGG - Chr5:98430661-98430680 MsG0580029549.01.T01:three_prime_UTR 50.0%
GCGCCCTTCTGAATACACAA+AGG + Chr5:98431308-98431327 None:intergenic 50.0%
TGTTCAACCTTTGTTGGCCG+AGG - Chr5:98430691-98430710 MsG0580029549.01.T01:three_prime_UTR 50.0%
!! CCGTGATAGCGGCCTATTGA+AGG - Chr5:98430715-98430734 MsG0580029549.01.T01:three_prime_UTR 55.0%
!! TCTGTGGTGTTGATGCGTGC+AGG - Chr5:98432013-98432032 MsG0580029549.01.T01:intron 55.0%
GGGCATCTGTGTTCTCGAGC+AGG + Chr5:98430979-98430998 None:intergenic 60.0%
TAGGCCGCTATCACGGACCT+CGG + Chr5:98430711-98430730 None:intergenic 60.0%
TCACGGACCTCGGCCAACAA+AGG + Chr5:98430701-98430720 None:intergenic 60.0%
TTGGCCGAGGTCCGTGATAG+CGG - Chr5:98430704-98430723 MsG0580029549.01.T01:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 98430584 98432381 98430584 ID=MsG0580029549.01;Name=MsG0580029549.01
Chr5 mRNA 98430584 98432381 98430584 ID=MsG0580029549.01.T01;Parent=MsG0580029549.01;Name=MsG0580029549.01.T01;_AED=0.50;_eAED=0.55;_QI=0|1|0.75|1|0.66|0.25|4|379|128
Chr5 exon 98430584 98430930 98430584 ID=MsG0580029549.01.T01:exon:25717;Parent=MsG0580029549.01.T01
Chr5 exon 98431346 98431470 98431346 ID=MsG0580029549.01.T01:exon:25716;Parent=MsG0580029549.01.T01
Chr5 exon 98431555 98431645 98431555 ID=MsG0580029549.01.T01:exon:25715;Parent=MsG0580029549.01.T01
Chr5 exon 98432179 98432381 98432179 ID=MsG0580029549.01.T01:exon:25714;Parent=MsG0580029549.01.T01
Chr5 CDS 98432179 98432381 98432179 ID=MsG0580029549.01.T01:cds;Parent=MsG0580029549.01.T01
Chr5 CDS 98431555 98431645 98431555 ID=MsG0580029549.01.T01:cds;Parent=MsG0580029549.01.T01
Chr5 CDS 98431378 98431470 98431378 ID=MsG0580029549.01.T01:cds;Parent=MsG0580029549.01.T01
Chr5 three_prime_UTR 98431346 98431377 98431346 ID=MsG0580029549.01.T01:three_prime_utr;Parent=MsG0580029549.01.T01
Chr5 three_prime_UTR 98430584 98430930 98430584 ID=MsG0580029549.01.T01:three_prime_utr;Parent=MsG0580029549.01.T01
Gene Sequence

>MsG0580029549.01.T01

ATGGTAGTCATGTGTCATCTCTCTATTCCTTTACTATATGATGCATTCTCATGCCCGTATTTTCGTTTTGATGTAGTGATGCCTCAGATGAAAGCCATGGCTCTAGATGTTCAACCTTTGTTGGCCGAGGTCCGTGATAGCGGCCTATTGAAGGAAGTTGAGAGTTTGACCCGAAGCCTTACCCTAGCTTCTGATGATTTGAGAAGGGCGCATTCAACGATTATGACCAATGAGAACGCTGAGCTGATCCAGAAGTCCATACACACACTTATATTTACCTTGAAGAACATTGAGAATATTACCTCTGATATGGTAGGTTTTACTGGCGATGAAAGTACCAAAAAGAATTTGAAACTACTTATCAAGAATCTCAGCAGGTTATTGTGA

Protein sequence

>MsG0580029549.01.T01

MVVMCHLSIPLLYDAFSCPYFRFDVVMPQMKAMALDVQPLLAEVRDSGLLKEVESLTRSLTLASDDLRRAHSTIMTNENAELIQKSIHTLIFTLKNIENITSDMVGFTGDESTKKNLKLLIKNLSRLL*