Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027092.01.T01 | GAU21477.1 | 88.298 | 94 | 11 | 0 | 1 | 94 | 1 | 94 | 7.19E-54 | 172 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027092.01.T01 | Q9C540 | 64.646 | 99 | 30 | 2 | 1 | 94 | 138 | 236 | 6.00E-28 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027092.01.T01 | A0A2Z6MXC8 | 88.298 | 94 | 11 | 0 | 1 | 94 | 1 | 94 | 3.44e-54 | 172 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000178.01 | MsG0580027092.01 | 0.821291 | 4.364531e-53 | 3.536282e-50 |
| MsG0180003361.01 | MsG0580027092.01 | 0.805858 | 1.089709e-49 | 5.827986e-47 |
| MsG0180004389.01 | MsG0580027092.01 | 0.800658 | 1.299973e-48 | 6.086953e-46 |
| MsG0280011388.01 | MsG0580027092.01 | 0.807476 | 4.960878e-50 | 2.765920e-47 |
| MsG0380012471.01 | MsG0580027092.01 | 0.816831 | 4.515646e-52 | 3.234227e-49 |
| MsG0380014537.01 | MsG0580027092.01 | 0.800334 | 1.512958e-48 | 7.026382e-46 |
| MsG0480020002.01 | MsG0580027092.01 | 0.830092 | 3.567470e-55 | 3.720952e-52 |
| MsG0480022213.01 | MsG0580027092.01 | -0.800816 | 1.206809e-48 | 5.673221e-46 |
| MsG0480023463.01 | MsG0580027092.01 | 0.803884 | 2.816843e-49 | 1.431917e-46 |
| MsG0480023707.01 | MsG0580027092.01 | 0.809863 | 1.533354e-50 | 9.104594e-48 |
| MsG0380015566.01 | MsG0580027092.01 | 0.801448 | 8.960779e-49 | 4.280475e-46 |
| MsG0380015631.01 | MsG0580027092.01 | 0.814488 | 1.501580e-51 | 1.008967e-48 |
| MsG0380015677.01 | MsG0580027092.01 | 0.802673 | 5.018391e-49 | 2.472950e-46 |
| MsG0380016919.01 | MsG0580027092.01 | 0.802776 | 4.778751e-49 | 2.361206e-46 |
| MsG0380016994.01 | MsG0580027092.01 | 0.801058 | 1.077091e-48 | 5.095008e-46 |
| MsG0580027091.01 | MsG0580027092.01 | 0.842092 | 3.193443e-58 | 4.789672e-55 |
| MsG0580027092.01 | MsG0780036477.01 | 0.803961 | 2.715244e-49 | 1.382982e-46 |
| MsG0580027092.01 | MsG0780036663.01 | 0.804566 | 2.031881e-49 | 1.051086e-46 |
| MsG0580027092.01 | MsG0780036815.01 | 0.818400 | 1.999306e-52 | 1.495105e-49 |
| MsG0580027092.01 | MsG0780037054.01 | 0.803032 | 4.230646e-49 | 2.103923e-46 |
| MsG0580027092.01 | MsG0780039137.01 | 0.811494 | 6.808325e-51 | 4.220777e-48 |
| MsG0580027092.01 | MsG0780039243.01 | 0.823536 | 1.313928e-53 | 1.134443e-50 |
| MsG0580027092.01 | MsG0780040841.01 | 0.800525 | 1.383655e-48 | 6.456809e-46 |
| MsG0580027092.01 | MsG0880041986.01 | 0.805623 | 1.220789e-49 | 6.489415e-47 |
| MsG0580027092.01 | MsG0880042698.01 | 0.820475 | 6.727307e-53 | 5.328406e-50 |
| MsG0580027092.01 | MsG0880045453.01 | 0.800068 | 1.713854e-48 | 7.905713e-46 |
| MsG0580027092.01 | MsG0880046012.01 | 0.840534 | 8.208724e-58 | 1.172724e-54 |
| MsG0580027092.01 | MsG0880046479.01 | 0.803868 | 2.839057e-49 | 1.442624e-46 |
| MsG0280006469.01 | MsG0580027092.01 | 0.838180 | 3.350879e-57 | 4.452535e-54 |
| MsG0280007392.01 | MsG0580027092.01 | 0.802276 | 6.057444e-49 | 2.955066e-46 |
| MsG0280007491.01 | MsG0580027092.01 | 0.805979 | 1.027685e-49 | 5.513240e-47 |
| MsG0280009748.01 | MsG0580027092.01 | 0.803122 | 4.053185e-49 | 2.020282e-46 |
| MsG0280009794.01 | MsG0580027092.01 | 0.828351 | 9.435285e-55 | 9.354261e-52 |
| MsG0280010489.01 | MsG0580027092.01 | 0.815950 | 7.107224e-52 | 4.969260e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027092.01.T01 | MTR_5g053250 | 92.553 | 94 | 7 | 0 | 1 | 94 | 91 | 184 | 1.39e-49 | 155 |
| MsG0580027092.01.T01 | MTR_5g053250 | 92.553 | 94 | 7 | 0 | 1 | 94 | 151 | 244 | 4.61e-49 | 155 |
| MsG0580027092.01.T01 | MTR_5g053250 | 92.553 | 94 | 7 | 0 | 1 | 94 | 178 | 271 | 1.15e-48 | 155 |
| MsG0580027092.01.T01 | MTR_6g017105 | 54.545 | 77 | 35 | 0 | 2 | 78 | 147 | 223 | 7.17e-25 | 93.6 |
| MsG0580027092.01.T01 | MTR_1g100643 | 45.455 | 66 | 35 | 1 | 9 | 74 | 100 | 164 | 1.16e-12 | 60.5 |
| MsG0580027092.01.T01 | MTR_1g100643 | 45.455 | 66 | 35 | 1 | 9 | 74 | 153 | 217 | 2.81e-12 | 60.5 |
| MsG0580027092.01.T01 | MTR_1g033360 | 44.595 | 74 | 36 | 1 | 3 | 76 | 149 | 217 | 1.05e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027092.01.T01 | AT1G26100 | 64.646 | 99 | 30 | 2 | 1 | 94 | 138 | 236 | 6.12e-29 | 103 |
| MsG0580027092.01.T01 | AT5G38630 | 50.000 | 82 | 38 | 1 | 2 | 80 | 147 | 228 | 1.32e-22 | 87.4 |
| MsG0580027092.01.T01 | AT4G25570 | 43.077 | 65 | 36 | 1 | 8 | 72 | 193 | 256 | 7.68e-11 | 57.0 |
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAAGTGGCTTTGTTATTT+TGG | 0.029119 | 5:+52989376 | MsG0580027092.01.T01:CDS |
| TTACCTTGGCATGTATTTCT+TGG | 0.229367 | 5:+52989212 | MsG0580027092.01.T01:CDS |
| GTTGTAAATTCTTTAGGCCT+TGG | 0.309741 | 5:+52989344 | MsG0580027092.01.T01:CDS |
| TCCATGGTTGTAAATTCTTT+AGG | 0.339651 | 5:+52989338 | MsG0580027092.01.T01:CDS |
| GCAGTAGCAACGGCGGAAAC+TGG | 0.361598 | 5:+52989254 | MsG0580027092.01.T01:CDS |
| CTGCCAATGCATAAGTGTAT+AGG | 0.426996 | 5:-52989235 | None:intergenic |
| CATGGACTCAGTGTTGTGTT+TGG | 0.439406 | 5:-52989321 | None:intergenic |
| CCAAAATAACAAAGCCACTT+AGG | 0.440273 | 5:-52989376 | None:intergenic |
| AAATGCGATCAGTGAGGATA+AGG | 0.461868 | 5:+52989186 | None:intergenic |
| CTTGGTCTTGCTCTCCTAAG+TGG | 0.482266 | 5:+52989362 | MsG0580027092.01.T01:CDS |
| CAAACACAACACTGAGTCCA+TGG | 0.527628 | 5:+52989322 | MsG0580027092.01.T01:CDS |
| TGGCCTATACACTTATGCAT+TGG | 0.552843 | 5:+52989232 | MsG0580027092.01.T01:CDS |
| AGGCCAAGAAATACATGCCA+AGG | 0.572983 | 5:-52989215 | None:intergenic |
| TTATGCATTGGCAGTAGCAA+CGG | 0.591173 | 5:+52989244 | MsG0580027092.01.T01:CDS |
| GCCTAAAGAATTTACAACCA+TGG | 0.598613 | 5:-52989339 | None:intergenic |
| TGAGGATAAGGATCTTACCT+TGG | 0.620818 | 5:+52989198 | MsG0580027092.01.T01:CDS |
| TGCATTGGCAGTAGCAACGG+CGG | 0.640059 | 5:+52989247 | MsG0580027092.01.T01:CDS |
| CACTTAGGAGAGCAAGACCA+AGG | 0.645769 | 5:-52989361 | None:intergenic |
| GAGGAGAAATGCGATCAGTG+AGG | 0.738861 | 5:+52989180 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGAAGAATTTGATACTT+AGG | - | Chr5:52989413-52989432 | None:intergenic | 20.0% |
| TCCATGGTTGTAAATTCTTT+AGG | + | Chr5:52989338-52989357 | MsG0580027092.01.T01:CDS | 30.0% | |
| ! | AACTTTTGTACTCAGATACT+AGG | + | Chr5:52989435-52989454 | MsG0580027092.01.T01:CDS | 30.0% |
| CCAAAATAACAAAGCCACTT+AGG | - | Chr5:52989379-52989398 | None:intergenic | 35.0% | |
| GCCTAAAGAATTTACAACCA+TGG | - | Chr5:52989342-52989361 | None:intergenic | 35.0% | |
| GTTGTAAATTCTTTAGGCCT+TGG | + | Chr5:52989344-52989363 | MsG0580027092.01.T01:CDS | 35.0% | |
| TTACCTTGGCATGTATTTCT+TGG | + | Chr5:52989212-52989231 | MsG0580027092.01.T01:CDS | 35.0% | |
| !!! | CCTAAGTGGCTTTGTTATTT+TGG | + | Chr5:52989376-52989395 | MsG0580027092.01.T01:CDS | 35.0% |
| CTGCCAATGCATAAGTGTAT+AGG | - | Chr5:52989238-52989257 | None:intergenic | 40.0% | |
| TGAGGATAAGGATCTTACCT+TGG | + | Chr5:52989198-52989217 | MsG0580027092.01.T01:CDS | 40.0% | |
| TGGCCTATACACTTATGCAT+TGG | + | Chr5:52989232-52989251 | MsG0580027092.01.T01:CDS | 40.0% | |
| ! | TTATGCATTGGCAGTAGCAA+CGG | + | Chr5:52989244-52989263 | MsG0580027092.01.T01:CDS | 40.0% |
| AGGCCAAGAAATACATGCCA+AGG | - | Chr5:52989218-52989237 | None:intergenic | 45.0% | |
| CAAACACAACACTGAGTCCA+TGG | + | Chr5:52989322-52989341 | MsG0580027092.01.T01:CDS | 45.0% | |
| CATGGACTCAGTGTTGTGTT+TGG | - | Chr5:52989324-52989343 | None:intergenic | 45.0% | |
| CACTTAGGAGAGCAAGACCA+AGG | - | Chr5:52989364-52989383 | None:intergenic | 50.0% | |
| !! | CTTGGTCTTGCTCTCCTAAG+TGG | + | Chr5:52989362-52989381 | MsG0580027092.01.T01:CDS | 50.0% |
| ! | TGCATTGGCAGTAGCAACGG+CGG | + | Chr5:52989247-52989266 | MsG0580027092.01.T01:CDS | 55.0% |
| GCAGTAGCAACGGCGGAAAC+TGG | + | Chr5:52989254-52989273 | MsG0580027092.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 52989188 | 52989472 | 52989188 | ID=MsG0580027092.01;Name=MsG0580027092.01 |
| Chr5 | mRNA | 52989188 | 52989472 | 52989188 | ID=MsG0580027092.01.T01;Parent=MsG0580027092.01;Name=MsG0580027092.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|94 |
| Chr5 | exon | 52989188 | 52989472 | 52989188 | ID=MsG0580027092.01.T01:exon:17439;Parent=MsG0580027092.01.T01 |
| Chr5 | CDS | 52989188 | 52989472 | 52989188 | ID=MsG0580027092.01.T01:cds;Parent=MsG0580027092.01.T01 |
| Gene Sequence |
| Protein sequence |
