AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880041986.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880041986.01.T01 MTR_8g008840 100.000 115 0 0 1 115 1 115 3.81e-83 238
MsG0880041986.01.T01 MTR_4g099050 52.830 106 47 2 1 105 38 141 4.96e-32 110
MsG0880041986.01.T01 MTR_5g013770 56.311 103 37 4 18 115 66 165 7.06e-31 107
MsG0880041986.01.T01 MTR_3g087700 53.571 56 25 1 32 87 118 172 1.11e-15 69.7
MsG0880041986.01.T01 MTR_8g022950 57.143 56 22 1 38 93 39 92 2.86e-15 67.0
MsG0880041986.01.T01 MTR_3g073960 48.148 54 28 0 29 82 81 134 3.07e-14 65.1
MsG0880041986.01.T01 MTR_2g042970 57.407 54 22 1 29 82 177 229 1.53e-13 64.7
MsG0880041986.01.T01 MTR_2g436020 44.444 72 31 2 14 78 35 104 2.90e-12 59.7
MsG0880041986.01.T01 MTR_2g436250 44.444 72 31 2 14 78 35 104 2.90e-12 59.7
MsG0880041986.01.T01 MTR_7g095170 53.659 41 19 0 37 77 223 263 7.38e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880041986.01.T01 AT2G44670 54.878 82 37 0 34 115 12 93 2.52e-29 102
MsG0880041986.01.T01 AT4G17670 47.368 114 41 4 16 115 51 159 4.95e-27 98.2
MsG0880041986.01.T01 AT5G47060 53.571 84 36 2 35 115 94 177 1.11e-25 95.1
MsG0880041986.01.T01 AT5G47060 53.571 84 36 2 35 115 94 177 1.11e-25 95.1
MsG0880041986.01.T01 AT1G78020 48.529 68 33 1 15 82 68 133 1.07e-17 74.3
MsG0880041986.01.T01 AT5G65040 47.541 61 30 1 33 93 54 112 2.04e-16 69.7
MsG0880041986.01.T01 AT1G22160 44.118 68 34 2 15 82 59 122 7.56e-15 66.6
MsG0880041986.01.T01 AT1G53885 55.319 47 21 0 35 81 39 85 3.67e-14 64.3
MsG0880041986.01.T01 AT1G53903 55.319 47 21 0 35 81 39 85 3.67e-14 64.3
MsG0880041986.01.T01 AT5G49120 50.000 46 23 0 33 78 63 108 3.51e-11 57.0
MsG0880041986.01.T01 AT5G20700 57.778 45 18 1 38 82 182 225 5.07e-11 58.2

Find 24 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAAAACATGCTAGATTT+TGG 0.214267 8:+2178309 None:intergenic
AGAATTTCTAACACCTCTTT+TGG 0.295222 8:+2177768 None:intergenic
ACATAAAGATGTCTTTGTTT+TGG 0.307802 8:+2178214 None:intergenic
GAGAAGGAGTTGGGAAAAGC+AGG 0.325602 8:+2178406 None:intergenic
TCAGAATTCTTCAAACAATA+AGG 0.335402 8:-2177737 MsG0880041986.01.T01:CDS
AGAGATTGAGAGAAGGAGTT+GGG 0.347307 8:+2178397 None:intergenic
ATTGTTTGAAGAATTCTGAT+TGG 0.437983 8:+2177741 None:intergenic
ATGAATCCAAAGAGAAAAGT+TGG 0.445108 8:-2177801 MsG0880041986.01.T01:CDS
TTCTACAAAGATAACAAGCT+TGG 0.448464 8:+2178247 None:intergenic
GGAGAAGGGATTGAAGAAGA+TGG 0.454187 8:+2178330 None:intergenic
GAGAGATTGAGAGAAGGAGT+TGG 0.470356 8:+2178396 None:intergenic
TGGAAGATTTCAACCAAAAG+AGG 0.483181 8:-2177781 MsG0880041986.01.T01:CDS
AAAGAATAGTTAGCAGCCAT+TGG 0.486211 8:+2178366 None:intergenic
ACAAAGACATCTTTATGTAC+AGG 0.489194 8:-2178209 MsG0880041986.01.T01:intron
TACAAAGATAACAAGCTTGG+AGG 0.501824 8:+2178250 None:intergenic
TATCTTTGTAGAAAACCTCT+TGG 0.507181 8:-2178237 MsG0880041986.01.T01:CDS
TGAGTTGAGAGATTGAGAGA+AGG 0.554490 8:+2178390 None:intergenic
CAATCTCTCAACTCAACCAA+TGG 0.563428 8:-2178382 MsG0880041986.01.T01:exon
CTGCACTCATTGCTACAGAA+TGG 0.599412 8:+2177844 None:intergenic
CATTCTGTAGCAATGAGTGC+AGG 0.607408 8:-2177843 MsG0880041986.01.T01:CDS
AAGATCAGAATCAGTTGCAG+TGG 0.640103 8:-2177710 MsG0880041986.01.T01:CDS
TAACAAGCTTGGAGGAAGTG+AGG 0.645660 8:+2178258 None:intergenic
ATGAAGATTCTGATAAAACA+AGG 0.650615 8:-2177589 MsG0880041986.01.T01:three_prime_UTR
AAAATCACATGACATATGAG+TGG 0.652107 8:-2177680 MsG0880041986.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGATGTAAATAATTATT+TGG - Chr8:2177943-2177962 MsG0880041986.01.T01:intron 10.0%
!! TGAAAAAAAAAATTATGAAG+TGG - Chr8:2178271-2178290 MsG0880041986.01.T01:CDS 15.0%
!! AAAGTATAAATATTCATCTG+GGG + Chr8:2177772-2177791 None:intergenic 20.0%
!! ACAAAGTATAAATATTCATC+TGG + Chr8:2177774-2177793 None:intergenic 20.0%
!! ATAGAAAAAAAAACTCAACA+AGG - Chr8:2178393-2178412 MsG0880041986.01.T01:five_prime_UTR 20.0%
!! CAAAGTATAAATATTCATCT+GGG + Chr8:2177773-2177792 None:intergenic 20.0%
!!! AAATCTAGCATGTTTTATTA+TGG - Chr8:2177603-2177622 MsG0880041986.01.T01:three_prime_UTR 20.0%
!!! TAATAAAACATGCTAGATTT+TGG + Chr8:2177603-2177622 None:intergenic 20.0%
!!! TATAATTGTGATTTTTCAGA+GGG - Chr8:2178036-2178055 MsG0880041986.01.T01:intron 20.0%
!!! TATGTCATGTGATTTTAATT+AGG + Chr8:2178225-2178244 None:intergenic 20.0%
!!! TTATAATTGTGATTTTTCAG+AGG - Chr8:2178035-2178054 MsG0880041986.01.T01:intron 20.0%
!!! TTTACACTTAATTTTCATGT+AGG - Chr8:2177794-2177813 MsG0880041986.01.T01:CDS 20.0%
! ATGAAGATTCTGATAAAACA+AGG - Chr8:2178320-2178339 MsG0880041986.01.T01:CDS 25.0%
! ATTGTTTGAAGAATTCTGAT+TGG + Chr8:2178171-2178190 None:intergenic 25.0%
! CCTCTTGTAAAAAAAATTGT+TGG - Chr8:2178357-2178376 MsG0880041986.01.T01:CDS 25.0%
! TCAGAATTCTTCAAACAATA+AGG - Chr8:2178172-2178191 MsG0880041986.01.T01:intron 25.0%
!! AAAAACTCAACAAGGAATTT+TGG - Chr8:2178401-2178420 MsG0880041986.01.T01:five_prime_UTR 25.0%
!! TTTTATCAGAATCTTCATGA+TGG + Chr8:2178318-2178337 None:intergenic 25.0%
!!! ACATAAAGATGTCTTTGTTT+TGG + Chr8:2177698-2177717 None:intergenic 25.0%
!!! CCAACAATTTTTTTTACAAG+AGG + Chr8:2178360-2178379 None:intergenic 25.0%
AAAATCACATGACATATGAG+TGG - Chr8:2178229-2178248 MsG0880041986.01.T01:CDS 30.0%
ACAAAGACATCTTTATGTAC+AGG - Chr8:2177700-2177719 MsG0880041986.01.T01:exon 30.0%
ATGAATCCAAAGAGAAAAGT+TGG - Chr8:2178108-2178127 MsG0880041986.01.T01:intron 30.0%
CAAAAGATGAAGGAAAATTG+AGG + Chr8:2177744-2177763 None:intergenic 30.0%
GTAGGTCATAACTCATAATA+AGG - Chr8:2177812-2177831 MsG0880041986.01.T01:CDS 30.0%
TATCTTTGTAGAAAACCTCT+TGG - Chr8:2177672-2177691 MsG0880041986.01.T01:three_prime_UTR 30.0%
TTCTACAAAGATAACAAGCT+TGG + Chr8:2177665-2177684 None:intergenic 30.0%
! AATCTTCCAACTTTTCTCTT+TGG + Chr8:2178117-2178136 None:intergenic 30.0%
! AGAATTTCTAACACCTCTTT+TGG + Chr8:2178144-2178163 None:intergenic 30.0%
!! TCTAGCATGTTTTATTATGG+TGG - Chr8:2177606-2177625 MsG0880041986.01.T01:three_prime_UTR 30.0%
AAAGAATAGTTAGCAGCCAT+TGG + Chr8:2177546-2177565 None:intergenic 35.0%
TACAAAGATAACAAGCTTGG+AGG + Chr8:2177662-2177681 None:intergenic 35.0%
TGGAAGATTTCAACCAAAAG+AGG - Chr8:2178128-2178147 MsG0880041986.01.T01:intron 35.0%
!! AACATGCTAGATTTTGGAGA+AGG + Chr8:2177597-2177616 None:intergenic 35.0%
!! ACATGCTAGATTTTGGAGAA+GGG + Chr8:2177596-2177615 None:intergenic 35.0%
AAGATCAGAATCAGTTGCAG+TGG - Chr8:2178199-2178218 MsG0880041986.01.T01:intron 40.0%
AGAGATTGAGAGAAGGAGTT+GGG + Chr8:2177515-2177534 None:intergenic 40.0%
CAATCTCTCAACTCAACCAA+TGG - Chr8:2177527-2177546 MsG0880041986.01.T01:three_prime_UTR 40.0%
! TGAGTTGAGAGATTGAGAGA+AGG + Chr8:2177522-2177541 None:intergenic 40.0%
!!! ATGTCTTTGTTTTGGCCAAG+AGG + Chr8:2177690-2177709 None:intergenic 40.0%
CATTCTGTAGCAATGAGTGC+AGG - Chr8:2178066-2178085 MsG0880041986.01.T01:intron 45.0%
CTGCACTCATTGCTACAGAA+TGG + Chr8:2178068-2178087 None:intergenic 45.0%
GAGAGATTGAGAGAAGGAGT+TGG + Chr8:2177516-2177535 None:intergenic 45.0%
GGAGAAGGGATTGAAGAAGA+TGG + Chr8:2177582-2177601 None:intergenic 45.0%
TAACAAGCTTGGAGGAAGTG+AGG + Chr8:2177654-2177673 None:intergenic 45.0%
TGGGGTGTGACAAAAGATGA+AGG + Chr8:2177754-2177773 None:intergenic 45.0%
!!! AGAATTTTTTTTTATTTATT+TGG - Chr8:2177974-2177993 MsG0880041986.01.T01:intron 5.0%
Chromosome Type Strat End Strand Name
Chr8 gene 2177506 2178425 2177506 ID=MsG0880041986.01;Name=MsG0880041986.01
Chr8 mRNA 2177506 2178425 2177506 ID=MsG0880041986.01.T01;Parent=MsG0880041986.01;Name=MsG0880041986.01.T01;_AED=0.29;_eAED=0.29;_QI=40|1|1|1|0|0|2|199|115
Chr8 exon 2177506 2177876 2177506 ID=MsG0880041986.01.T01:exon:1742;Parent=MsG0880041986.01.T01
Chr8 exon 2178210 2178425 2178210 ID=MsG0880041986.01.T01:exon:1741;Parent=MsG0880041986.01.T01
Chr8 five_prime_UTR 2178386 2178425 2178386 ID=MsG0880041986.01.T01:five_prime_utr;Parent=MsG0880041986.01.T01
Chr8 CDS 2178210 2178385 2178210 ID=MsG0880041986.01.T01:cds;Parent=MsG0880041986.01.T01
Chr8 CDS 2177705 2177876 2177705 ID=MsG0880041986.01.T01:cds;Parent=MsG0880041986.01.T01
Chr8 three_prime_UTR 2177506 2177704 2177506 ID=MsG0880041986.01.T01:three_prime_utr;Parent=MsG0880041986.01.T01
Gene Sequence

>MsG0880041986.01.T01

ATGGCTGCTAACTATTCTTTCTCTTCTTCATCACCATCTTCTTCAATCCCTTCTCCAAAATCTAGCATGTTTTATTATGGTGGAAGTGAAGATTTCTATGATGAACCTCACTTCCTCCAAGCTTGTTATCTTTGTAGAAAACCTCTTGGCCAAAACAAAGACATCTTTATGTACAGAGGGAACACACCATTCTGTAGCAATGAGTGCAGGCAAGAACAAATAGAGATTGATGAATCCAAAGAGAAAAGTTGGAAGATTTCAACCAAAAGAGGTGTTAGAAATTCTGAAACCAATCAGAATTCTTCAAACAATAAGGCTGTAAGATCAGAATCAGTTGCAGTGGCCTAA

Protein sequence

>MsG0880041986.01.T01

MAANYSFSSSSPSSSIPSPKSSMFYYGGSEDFYDEPHFLQACYLCRKPLGQNKDIFMYRGNTPFCSNECRQEQIEIDESKEKSWKISTKRGVRNSETNQNSSNNKAVRSESVAVA*