Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001187.01.T01 | XP_013466600.1 | 82.828 | 99 | 11 | 2 | 4 | 96 | 473 | 571 | 5.77E-35 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001187.01.T01 | Q9LQV2 | 59.783 | 92 | 29 | 3 | 13 | 96 | 475 | 566 | 1.26E-23 | 96.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001187.01.T01 | A0A072VFQ4 | 82.828 | 99 | 11 | 2 | 4 | 96 | 473 | 571 | 2.76e-35 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000091.01 | MsG0180001187.01 | 0.813289 | 2.760579e-51 | 1.795422e-48 |
| MsG0180000652.01 | MsG0180001187.01 | 0.807592 | 4.688704e-50 | 2.621853e-47 |
| MsG0180001187.01 | MsG0180002259.01 | 0.823432 | 1.389899e-53 | 1.196470e-50 |
| MsG0180001187.01 | MsG0180004275.01 | 0.813294 | 2.753108e-51 | 1.790837e-48 |
| MsG0180001187.01 | MsG0180004765.01 | 0.806628 | 7.500784e-50 | 4.090750e-47 |
| MsG0180001187.01 | MsG0180005730.01 | 0.802610 | 5.169878e-49 | 2.543562e-46 |
| MsG0180001187.01 | MsG0180006134.01 | 0.810726 | 9.987081e-51 | 6.066656e-48 |
| MsG0180001187.01 | MsG0280006309.01 | 0.805535 | 1.274030e-49 | 6.756872e-47 |
| MsG0180001187.01 | MsG0280006601.01 | 0.812066 | 5.110773e-51 | 3.216893e-48 |
| MsG0180001187.01 | MsG0280007118.01 | 0.801433 | 9.024579e-49 | 4.309293e-46 |
| MsG0180001187.01 | MsG0280007955.01 | 0.822858 | 1.891290e-53 | 1.602062e-50 |
| MsG0180001187.01 | MsG0280008377.01 | 0.812222 | 4.725382e-51 | 2.987229e-48 |
| MsG0180001187.01 | MsG0280009421.01 | 0.835083 | 2.062286e-56 | 2.494948e-53 |
| MsG0180001187.01 | MsG0280010349.01 | 0.804299 | 2.309613e-49 | 1.186584e-46 |
| MsG0180001187.01 | MsG0280010350.01 | 0.803742 | 3.014591e-49 | 1.526806e-46 |
| MsG0180001187.01 | MsG0280010517.01 | 0.802947 | 4.405384e-49 | 2.186061e-46 |
| MsG0180001187.01 | MsG0280010722.01 | 0.811198 | 7.891207e-51 | 4.853925e-48 |
| MsG0180001187.01 | MsG0280010807.01 | 0.816649 | 4.958743e-52 | 3.534119e-49 |
| MsG0180001187.01 | MsG0380012103.01 | 0.810115 | 1.353137e-50 | 8.087943e-48 |
| MsG0180001187.01 | MsG0380014495.01 | 0.816198 | 6.257719e-52 | 4.405257e-49 |
| MsG0180001187.01 | MsG0380014948.01 | 0.809815 | 1.570445e-50 | 9.313311e-48 |
| MsG0180001187.01 | MsG0380015039.01 | 0.803523 | 3.347205e-49 | 1.685823e-46 |
| MsG0180001187.01 | MsG0380016285.01 | 0.813223 | 2.854273e-51 | 1.852934e-48 |
| MsG0180001187.01 | MsG0480018465.01 | 0.810721 | 1.001351e-50 | 6.081899e-48 |
| MsG0180001187.01 | MsG0480018653.01 | 0.804480 | 2.116554e-49 | 1.092472e-46 |
| MsG0180001187.01 | MsG0480020765.01 | 0.808494 | 3.012737e-50 | 1.725147e-47 |
| MsG0180001187.01 | MsG0480021918.01 | 0.801276 | 9.718633e-49 | 4.622419e-46 |
| MsG0180001187.01 | MsG0480022068.01 | 0.802223 | 6.211284e-49 | 3.025953e-46 |
| MsG0180001187.01 | MsG0480023045.01 | 0.806756 | 7.048695e-50 | 3.857052e-47 |
| MsG0180001187.01 | MsG0480023390.01 | 0.805583 | 1.244576e-49 | 6.609106e-47 |
| MsG0180001187.01 | MsG0480023488.01 | 0.814617 | 1.406059e-51 | 9.480448e-49 |
| MsG0180001187.01 | MsG0480023489.01 | 0.801800 | 7.588096e-49 | 3.657147e-46 |
| MsG0180001187.01 | MsG0580024038.01 | 0.800846 | 1.189837e-48 | 5.598035e-46 |
| MsG0180001187.01 | MsG0580025332.01 | 0.810574 | 1.077294e-50 | 6.517267e-48 |
| MsG0180001187.01 | MsG0580025723.01 | 0.801723 | 7.871842e-49 | 3.786304e-46 |
| MsG0180001187.01 | MsG0580028692.01 | 0.805941 | 1.046559e-49 | 5.609061e-47 |
| MsG0180001187.01 | MsG0580029880.01 | 0.800372 | 1.486542e-48 | 6.910393e-46 |
| MsG0180001187.01 | MsG0580029941.01 | 0.801470 | 8.868216e-49 | 4.238537e-46 |
| MsG0180001187.01 | MsG0680030616.01 | 0.810520 | 1.106677e-50 | 6.685369e-48 |
| MsG0180001187.01 | MsG0680032157.01 | 0.806554 | 7.774545e-50 | 4.232448e-47 |
| MsG0180001187.01 | MsG0680032665.01 | 0.815167 | 1.061862e-51 | 7.266369e-49 |
| MsG0180001187.01 | MsG0680034013.01 | 0.807732 | 4.377565e-50 | 2.457004e-47 |
| MsG0180001187.01 | MsG0680035123.01 | 0.801343 | 9.418423e-49 | 4.487122e-46 |
| MsG0180001187.01 | MsG0780036593.01 | 0.806688 | 7.285299e-50 | 3.979497e-47 |
| MsG0180001187.01 | MsG0780040074.01 | 0.820555 | 6.446569e-53 | 5.117464e-50 |
| MsG0180001187.01 | MsG0780041416.01 | 0.807242 | 5.562991e-50 | 3.082805e-47 |
| MsG0180001187.01 | MsG0880041861.01 | 0.812189 | 4.804814e-51 | 3.034595e-48 |
| MsG0180001187.01 | MsG0880041863.01 | 0.804071 | 2.575250e-49 | 1.315418e-46 |
| MsG0180001187.01 | MsG0880041864.01 | 0.805458 | 1.322029e-49 | 6.997975e-47 |
| MsG0180001187.01 | MsG0880042656.01 | 0.802902 | 4.501006e-49 | 2.231053e-46 |
| MsG0180001187.01 | MsG0880043157.01 | 0.818564 | 1.835557e-52 | 1.378733e-49 |
| MsG0180001187.01 | MsG0880043521.01 | 0.802583 | 5.237104e-49 | 2.574874e-46 |
| MsG0180001187.01 | MsG0880043941.01 | 0.813632 | 2.319548e-51 | 1.522784e-48 |
| MsG0180001187.01 | MsG0880045111.01 | 0.807406 | 5.133174e-50 | 2.856833e-47 |
| MsG0180001187.01 | MsG0880045179.01 | 0.800242 | 1.580222e-48 | 7.321261e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001187.01.T01 | MTR_1g032610 | 82.828 | 99 | 11 | 2 | 4 | 96 | 473 | 571 | 6.99e-39 | 137 |
| MsG0180001187.01.T01 | MTR_1g032610 | 82.828 | 99 | 11 | 2 | 4 | 96 | 410 | 508 | 7.32e-39 | 137 |
| MsG0180001187.01.T01 | MTR_1g032720 | 70.588 | 102 | 21 | 3 | 4 | 96 | 470 | 571 | 2.75e-36 | 129 |
| MsG0180001187.01.T01 | MTR_1g032720 | 70.588 | 102 | 21 | 3 | 4 | 96 | 1079 | 1180 | 2.75e-36 | 129 |
| MsG0180001187.01.T01 | MTR_1g032630 | 68.627 | 102 | 23 | 3 | 4 | 96 | 467 | 568 | 2.56e-35 | 127 |
| MsG0180001187.01.T01 | MTR_1g032630 | 68.627 | 102 | 23 | 3 | 4 | 96 | 467 | 568 | 3.62e-35 | 126 |
| MsG0180001187.01.T01 | MTR_1g032590 | 58.333 | 96 | 31 | 3 | 10 | 96 | 466 | 561 | 3.75e-25 | 98.2 |
| MsG0180001187.01.T01 | MTR_4g106660 | 43.478 | 92 | 43 | 3 | 14 | 96 | 491 | 582 | 9.37e-16 | 71.2 |
| MsG0180001187.01.T01 | MTR_4g106660 | 43.478 | 92 | 43 | 3 | 14 | 96 | 491 | 582 | 9.60e-16 | 71.2 |
| MsG0180001187.01.T01 | MTR_3g107390 | 43.299 | 97 | 49 | 3 | 5 | 96 | 524 | 619 | 2.98e-14 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001187.01.T01 | AT1G14790 | 59.783 | 92 | 29 | 3 | 13 | 96 | 475 | 566 | 1.28e-24 | 96.7 |
| MsG0180001187.01.T01 | AT4G11130 | 46.667 | 90 | 41 | 2 | 14 | 96 | 495 | 584 | 8.93e-18 | 77.0 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATCAGAGAAGACATATT+TGG | 0.262792 | 1:-16968587 | None:intergenic |
| ATCGAGAATAATTTCAATTT+CGG | 0.275279 | 1:-16968551 | None:intergenic |
| TATGTCTTCTCTGATGGAAT+TGG | 0.304418 | 1:+16968592 | MsG0180001187.01.T01:CDS |
| TTCAAAACATGCTAATGCTT+TGG | 0.309951 | 1:+16968398 | None:intergenic |
| ATCACCCATCCATTCCCTTA+TGG | 0.311189 | 1:-16968452 | None:intergenic |
| TTCGCTACAACAACCGAATC+TGG | 0.342587 | 1:-16968684 | None:intergenic |
| TGCCGTCTCCCTTCCAGATT+CGG | 0.351921 | 1:+16968671 | MsG0180001187.01.T01:CDS |
| GCTGCTGACCCTTGTAGTTT+CGG | 0.378895 | 1:-16968527 | None:intergenic |
| ATGTCTTCTCTGATGGAATT+GGG | 0.442636 | 1:+16968593 | MsG0180001187.01.T01:CDS |
| ACCAAATATGTCTTCTCTGA+TGG | 0.456825 | 1:+16968586 | MsG0180001187.01.T01:CDS |
| GAGATACCGCTGGTTCCATA+AGG | 0.476868 | 1:+16968437 | MsG0180001187.01.T01:CDS |
| AAATATCTCTTGAACTTGCC+AGG | 0.478082 | 1:+16968617 | MsG0180001187.01.T01:CDS |
| GTTCCTCTACCGAAACTACA+AGG | 0.497463 | 1:+16968518 | MsG0180001187.01.T01:CDS |
| TGGTTCCATAAGGGAATGGA+TGG | 0.513293 | 1:+16968447 | MsG0180001187.01.T01:CDS |
| GACCCTTGTAGTTTCGGTAG+AGG | 0.518386 | 1:-16968521 | None:intergenic |
| CCGCTGGTTCCATAAGGGAA+TGG | 0.558952 | 1:+16968443 | MsG0180001187.01.T01:CDS |
| AGATACCGCTGGTTCCATAA+GGG | 0.560391 | 1:+16968438 | MsG0180001187.01.T01:CDS |
| TATGTCGCTGGAGATACCGC+TGG | 0.578113 | 1:+16968427 | MsG0180001187.01.T01:CDS |
| CTACAACAACCGAATCTGGA+AGG | 0.578903 | 1:-16968680 | None:intergenic |
| AACCGAATCTGGAAGGGAGA+CGG | 0.583445 | 1:-16968673 | None:intergenic |
| TCTTGAACTTGCCAGGAGAG+TGG | 0.617568 | 1:+16968624 | MsG0180001187.01.T01:CDS |
| TACAACAACCGAATCTGGAA+GGG | 0.624487 | 1:-16968679 | None:intergenic |
| CCATTCCCTTATGGAACCAG+CGG | 0.630594 | 1:-16968443 | None:intergenic |
| TTCCTCTACCGAAACTACAA+GGG | 0.683599 | 1:+16968519 | MsG0180001187.01.T01:CDS |
| GGTTCCATAAGGGAATGGAT+GGG | 0.684184 | 1:+16968448 | MsG0180001187.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCGAGAATAATTTCAATTT+CGG | - | Chr1:16968554-16968573 | None:intergenic | 20.0% |
| !!! | AGAATCATAATCACATTTTT+TGG | - | Chr1:16968650-16968669 | None:intergenic | 20.0% |
| !!! | ATTTAGCTACATTTTTTATC+CGG | - | Chr1:16968483-16968502 | None:intergenic | 20.0% |
| AAATATCTCTTGAACTTGCC+AGG | + | Chr1:16968617-16968636 | MsG0180001187.01.T01:CDS | 35.0% | |
| ACCAAATATGTCTTCTCTGA+TGG | + | Chr1:16968586-16968605 | MsG0180001187.01.T01:CDS | 35.0% | |
| ATGTCTTCTCTGATGGAATT+GGG | + | Chr1:16968593-16968612 | MsG0180001187.01.T01:CDS | 35.0% | |
| TATGTCTTCTCTGATGGAAT+TGG | + | Chr1:16968592-16968611 | MsG0180001187.01.T01:CDS | 35.0% | |
| TCCATCAGAGAAGACATATT+TGG | - | Chr1:16968590-16968609 | None:intergenic | 35.0% | |
| !! | CTTTGGAAATTTTATGTCGC+TGG | + | Chr1:16968415-16968434 | MsG0180001187.01.T01:CDS | 35.0% |
| TACAACAACCGAATCTGGAA+GGG | - | Chr1:16968682-16968701 | None:intergenic | 40.0% | |
| TTCCTCTACCGAAACTACAA+GGG | + | Chr1:16968519-16968538 | MsG0180001187.01.T01:CDS | 40.0% | |
| !! | AATGGATGGGTGATTTTAGC+CGG | + | Chr1:16968461-16968480 | MsG0180001187.01.T01:CDS | 40.0% |
| ATCACCCATCCATTCCCTTA+TGG | - | Chr1:16968455-16968474 | None:intergenic | 45.0% | |
| CTACAACAACCGAATCTGGA+AGG | - | Chr1:16968683-16968702 | None:intergenic | 45.0% | |
| GGTTCCATAAGGGAATGGAT+GGG | + | Chr1:16968448-16968467 | MsG0180001187.01.T01:CDS | 45.0% | |
| GTTCCTCTACCGAAACTACA+AGG | + | Chr1:16968518-16968537 | MsG0180001187.01.T01:CDS | 45.0% | |
| TGGTTCCATAAGGGAATGGA+TGG | + | Chr1:16968447-16968466 | MsG0180001187.01.T01:CDS | 45.0% | |
| !! | AGATACCGCTGGTTCCATAA+GGG | + | Chr1:16968438-16968457 | MsG0180001187.01.T01:CDS | 45.0% |
| !!! | ACATTTTTTGGCCACTCTCC+TGG | - | Chr1:16968638-16968657 | None:intergenic | 45.0% |
| AACCGAATCTGGAAGGGAGA+CGG | - | Chr1:16968676-16968695 | None:intergenic | 50.0% | |
| CCATTCCCTTATGGAACCAG+CGG | - | Chr1:16968446-16968465 | None:intergenic | 50.0% | |
| GACCCTTGTAGTTTCGGTAG+AGG | - | Chr1:16968524-16968543 | None:intergenic | 50.0% | |
| GCTGCTGACCCTTGTAGTTT+CGG | - | Chr1:16968530-16968549 | None:intergenic | 50.0% | |
| TCTTGAACTTGCCAGGAGAG+TGG | + | Chr1:16968624-16968643 | MsG0180001187.01.T01:CDS | 50.0% | |
| !! | GAGATACCGCTGGTTCCATA+AGG | + | Chr1:16968437-16968456 | MsG0180001187.01.T01:CDS | 50.0% |
| TATGTCGCTGGAGATACCGC+TGG | + | Chr1:16968427-16968446 | MsG0180001187.01.T01:CDS | 55.0% | |
| TGCCGTCTCCCTTCCAGATT+CGG | + | Chr1:16968671-16968690 | MsG0180001187.01.T01:CDS | 55.0% | |
| !! | CCGCTGGTTCCATAAGGGAA+TGG | + | Chr1:16968443-16968462 | MsG0180001187.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 16968406 | 16968702 | 16968406 | ID=MsG0180001187.01;Name=MsG0180001187.01 |
| Chr1 | mRNA | 16968406 | 16968702 | 16968406 | ID=MsG0180001187.01.T01;Parent=MsG0180001187.01;Name=MsG0180001187.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr1 | exon | 16968406 | 16968702 | 16968406 | ID=MsG0180001187.01.T01:exon:31590;Parent=MsG0180001187.01.T01 |
| Chr1 | CDS | 16968406 | 16968702 | 16968406 | ID=MsG0180001187.01.T01:cds;Parent=MsG0180001187.01.T01 |
| Gene Sequence |
| Protein sequence |
