AlfalfaGEDB | MsG0880046760.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046760.01

KEGG: K12845
GO: GO:0003723 GO:0005730 GO:0030529 GO:0042254
PFAM: PF01248

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046760.01.T01	XP_003609312.1	100	127	0	0	6	132	2	128	1.19E-86	258

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046760.01.T01	Q5XH16	81.89	127	23	0	6	132	2	128	7.72E-74	218

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046760.01.T01	A0A1S2YQW0	100.000	127	0	0	6	132	2	128	5.68e-87	258

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048922.01	MsG0880046760.01	0.838360	3.011959e-57	4.024546e-54
MsG0880046486.01	MsG0880046760.01	0.840696	7.441512e-58	1.068521e-54
MsG0180002738.01	MsG0880046760.01	0.831855	1.317509e-55	1.447674e-52
MsG0180003585.01	MsG0880046760.01	0.891966	2.449443e-74	2.193649e-70
MsG0180003897.01	MsG0880046760.01	0.817574	3.073005e-52	2.246196e-49
MsG0180004946.01	MsG0880046760.01	0.818624	1.778424e-52	1.338007e-49
MsG0180005789.01	MsG0880046760.01	0.813165	2.939094e-51	1.904959e-48
MsG0280006301.01	MsG0880046760.01	0.808269	3.364899e-50	1.915539e-47
MsG0780041244.01	MsG0880046760.01	0.808029	3.786258e-50	2.141638e-47
MsG0780041461.01	MsG0880046760.01	0.817987	2.479306e-52	1.832896e-49
MsG0780041522.01	MsG0880046760.01	0.881284	2.728592e-70	1.588800e-66
MsG0880042517.01	MsG0880046760.01	0.839744	1.319154e-57	1.838803e-54
MsG0880042896.01	MsG0880046760.01	0.812840	3.462987e-51	2.225058e-48
MsG0880043076.01	MsG0880046760.01	0.899513	1.839982e-77	2.283194e-73
MsG0680031368.01	MsG0880046760.01	0.813175	2.924168e-51	1.895819e-48
MsG0680032459.01	MsG0880046760.01	0.863294	2.759634e-64	8.324459e-61
MsG0680035611.01	MsG0880046760.01	0.831305	1.800339e-55	1.945880e-52
MsG0480020796.01	MsG0880046760.01	0.807100	5.959961e-50	3.290413e-47
MsG0480020911.01	MsG0880046760.01	0.832555	8.841279e-56	9.916819e-53
MsG0480021279.01	MsG0880046760.01	0.865108	7.497314e-65	2.410807e-61
MsG0480021288.01	MsG0880046760.01	0.801739	7.811816e-49	3.758922e-46
MsG0480021417.01	MsG0880046760.01	0.850787	1.354605e-60	2.683973e-57
MsG0480021879.01	MsG0880046760.01	0.819409	1.179221e-52	9.066977e-50
MsG0480022086.01	MsG0880046760.01	0.835523	1.596773e-56	1.957516e-53
MsG0480022363.01	MsG0880046760.01	0.839468	1.556025e-57	2.150823e-54
MsG0480023050.01	MsG0880046760.01	0.806765	7.016079e-50	3.840128e-47
MsG0480023380.01	MsG0880046760.01	0.805084	1.583428e-49	8.301313e-47
MsG0480023931.01	MsG0880046760.01	0.859708	3.430572e-63	9.144149e-60
MsG0480023996.01	MsG0880046760.01	0.824949	6.115887e-54	5.497726e-51
MsG0580024540.01	MsG0880046760.01	0.832613	8.553552e-56	9.610517e-53
MsG0580024738.01	MsG0880046760.01	0.866576	2.578118e-65	8.731787e-62
MsG0580025750.01	MsG0880046760.01	0.846191	2.536136e-59	4.330083e-56
MsG0380016678.01	MsG0880046760.01	0.852093	5.786586e-61	1.195977e-57
MsG0380016744.01	MsG0880046760.01	0.815961	7.070210e-52	4.944785e-49
MsG0380016760.01	MsG0880046760.01	0.830817	2.371545e-55	2.526628e-52
MsG0580029047.01	MsG0880046760.01	0.857241	1.866526e-62	4.576430e-59
MsG0580030073.01	MsG0880046760.01	0.809167	2.163135e-50	1.260912e-47
MsG0280006629.01	MsG0880046760.01	0.826629	2.442105e-54	2.302729e-51
MsG0280006747.01	MsG0880046760.01	0.829962	3.837837e-55	3.988222e-52
MsG0280007055.01	MsG0880046760.01	0.809990	1.439488e-50	8.576254e-48
MsG0280007606.01	MsG0880046760.01	0.816752	4.704082e-52	3.361965e-49
MsG0280007995.01	MsG0880046760.01	0.807916	4.000009e-50	2.255775e-47
MsG0280010519.01	MsG0880046760.01	0.805921	1.056674e-49	5.660263e-47
MsG0780038383.01	MsG0880046760.01	0.824344	8.495191e-54	7.503803e-51

PPI

Gene1	Gene2	Type
MsG0880046760.01	MsG0880047038.01	PPI
MsG0280009166.01	MsG0880046760.01	PPI
MsG0380015857.01	MsG0880046760.01	PPI
MsG0480023399.01	MsG0880046760.01	PPI
MsG0480021288.01	MsG0880046760.01	PPI
MsG0880046760.01	MsG0880046575.01	PPI
MsG0380012898.01	MsG0880046760.01	PPI
MsG0280008557.01	MsG0880046760.01	PPI
MsG0880046760.01	MsG0180006082.01	PPI
MsG0880046760.01	MsG0280006747.01	PPI
MsG0580024916.01	MsG0880046760.01	PPI
MsG0880046575.01	MsG0880046760.01	PPI
MsG0180001832.01	MsG0880046760.01	PPI
MsG0280008503.01	MsG0880046760.01	PPI
MsG0880046760.01	MsG0180001488.01	PPI
MsG0880046760.01	MsG0880046243.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046760.01.T01	MTR_4g114330	100.000	127	0	0	6	132	2	128	1.44e-90	258
MsG0880046760.01.T01	MTR_0011s0330	97.638	127	3	0	6	132	2	128	3.80e-88	252
MsG0880046760.01.T01	MTR_4g112780	34.483	116	75	1	8	122	17	132	1.61e-17	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046760.01.T01	AT4G22380	93.701	127	8	0	6	132	2	128	7.86e-76	221
MsG0880046760.01.T01	AT5G20160	92.913	127	9	0	6	132	2	128	4.91e-75	219
MsG0880046760.01.T01	AT4G12600	89.764	127	13	0	6	132	2	128	1.08e-71	211
MsG0880046760.01.T01	AT5G20160	74.214	159	9	1	6	132	2	160	2.45e-68	203
MsG0880046760.01.T01	AT4G12600	55.495	182	26	2	6	132	2	183	2.35e-48	153
MsG0880046760.01.T01	AT5G20160	65.421	107	5	1	6	80	2	108	8.39e-31	106
MsG0880046760.01.T01	AT5G08180	35.938	128	73	1	12	130	24	151	3.85e-21	83.6
MsG0880046760.01.T01	AT5G08180	35.938	128	73	1	12	130	24	151	3.85e-21	83.6

Find 27 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACTATAAGCAACTCAAAA+AGG	0.236397	8:+77747474	MsG0880046760.01.T01:CDS
TATTTCTGAATTTGTTGTTA+TGG	0.302213	8:+77747620	MsG0880046760.01.T01:CDS
TTGTTGAACAAGATCCATTA+TGG	0.342840	8:-77747446	None:intergenic
AGCTACTAAGACCTTGAATA+GGG	0.384258	8:+77747596	MsG0880046760.01.T01:intron
TCAGCCAAAGGGTATGCCTT+AGG	0.396497	8:-77747406	None:intergenic
TCCTCTACTTGCTGAGGATA+AGG	0.406375	8:+77747680	MsG0880046760.01.T01:CDS
CAGCTACTAAGACCTTGAAT+AGG	0.416543	8:+77747595	MsG0880046760.01.T01:intron
TTCAGAAATACCCCTATTCA+AGG	0.432400	8:-77747607	None:intergenic
TAACCCTAAGGCATACCCTT+TGG	0.467615	8:+77747402	MsG0880046760.01.T01:CDS
TCAGCTAACAATCACCATAA+TGG	0.473714	8:+77747432	MsG0880046760.01.T01:CDS
CAGCCAAAGGGTATGCCTTA+GGG	0.474338	8:-77747405	None:intergenic
CCTCAGCAAGTAGAGGAAGA+TGG	0.517598	8:-77747674	None:intergenic
AGAACAAGCAATCACTGGTC+TGG	0.520395	8:-77748510	None:intergenic
CCATCTTCCTCTACTTGCTG+AGG	0.525035	8:+77747674	MsG0880046760.01.T01:CDS
GTCACATCAAAACAAGCACT+TGG	0.533789	8:+77748470	MsG0880046760.01.T01:CDS
TTTGATGTGACGAACACATA+GGG	0.556996	8:-77748458	None:intergenic
GTCACAGAACAAGCAATCAC+TGG	0.559563	8:-77748515	None:intergenic
AGGAGAAGCAGTTAACCCTA+AGG	0.573343	8:+77747390	MsG0880046760.01.T01:CDS
CAAGCACTTGGGCGTGCTTG+TGG	0.582426	8:+77748482	MsG0880046760.01.T01:CDS
TTAGCTGAGCATCAGCCAAA+GGG	0.590874	8:-77747417	None:intergenic
AGATGGAGAAGAATCTCAAG+AGG	0.597462	8:-77747657	None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG	0.598758	8:-77747418	None:intergenic
GTCTCAGATTCAGCAACTCA+AGG	0.599622	8:+77748562	MsG0880046760.01.T01:CDS
ACCTTATCCTCAGCAAGTAG+AGG	0.612122	8:-77747681	None:intergenic
TGTTCTGTGACAACTAATGA+AGG	0.613805	8:+77748527	MsG0880046760.01.T01:CDS
GCTACTAAGACCTTGAATAG+GGG	0.659558	8:+77747597	MsG0880046760.01.T01:intron
TCACATCAAAACAAGCACTT+GGG	0.661984	8:+77748471	MsG0880046760.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATGAAATAAGAAATT+AGG	-	Chr8:77747517-77747536	None:intergenic	10.0%
!!	AAAAATGAAATAAGAAATTA+GGG	-	Chr8:77747516-77747535	None:intergenic	10.0%
!!	AATATTGAAATAAAATGAAT+CGG	-	Chr8:77748359-77748378	None:intergenic	10.0%
!!	AATTTACACAATTTATAATA+AGG	+	Chr8:77748003-77748022	MsG0880046760.01.T01:intron	10.0%
!!	ATTAATCAATCAATAATCAA+AGG	-	Chr8:77747558-77747577	None:intergenic	15.0%
!!	TAAATTGTGTAAATTAGTAT+AGG	-	Chr8:77747998-77748017	None:intergenic	15.0%
!!!	ATTTTAGTTTTTTCATAACT+TGG	+	Chr8:77747826-77747845	MsG0880046760.01.T01:intron	15.0%
!!	ATTGTTAAAAAATAAGAGCT+TGG	-	Chr8:77747808-77747827	None:intergenic	20.0%
!!	GAAAATGAATTCTGAATATT+TGG	+	Chr8:77748656-77748675	MsG0880046760.01.T01:intron	20.0%
!!!	TATTTCTGAATTTGTTGTTA+TGG	+	Chr8:77747620-77747639	MsG0880046760.01.T01:CDS	20.0%
!	AACTATAAGCAACTCAAAAA+GGG	+	Chr8:77747475-77747494	MsG0880046760.01.T01:CDS	25.0%
!	AGTAATATTACTTGAAGACA+TGG	-	Chr8:77748237-77748256	None:intergenic	25.0%
!	ATAAAATGAATCGGATGAAA+TGG	-	Chr8:77748350-77748369	None:intergenic	25.0%
!	ATGAATTCTGAATATTTGGT+TGG	+	Chr8:77748660-77748679	MsG0880046760.01.T01:intron	25.0%
!	TAACTATAAGCAACTCAAAA+AGG	+	Chr8:77747474-77747493	MsG0880046760.01.T01:CDS	25.0%
	AAACAGGGTTATGTTATGTT+TGG	+	Chr8:77748043-77748062	MsG0880046760.01.T01:intron	30.0%
	AACAGGGTTATGTTATGTTT+GGG	+	Chr8:77748044-77748063	MsG0880046760.01.T01:intron	30.0%
	TGTTATGCATAGCATCTTAT+TGG	+	Chr8:77747931-77747950	MsG0880046760.01.T01:intron	30.0%
	TTGTATCCATACAGATGTTT+GGG	+	Chr8:77747963-77747982	MsG0880046760.01.T01:intron	30.0%
	TTGTTGAACAAGATCCATTA+TGG	-	Chr8:77747449-77747468	None:intergenic	30.0%
	TTTGTATCCATACAGATGTT+TGG	+	Chr8:77747962-77747981	MsG0880046760.01.T01:intron	30.0%
!!!	GATCGATGACTTTTAAAACA+GGG	+	Chr8:77748028-77748047	MsG0880046760.01.T01:intron	30.0%
!!!	TGATCGATGACTTTTAAAAC+AGG	+	Chr8:77748027-77748046	MsG0880046760.01.T01:intron	30.0%
	AGCTACTAAGACCTTGAATA+GGG	+	Chr8:77747596-77747615	MsG0880046760.01.T01:intron	35.0%
	CATGGTTTATCACACTTGTT+TGG	-	Chr8:77748219-77748238	None:intergenic	35.0%
	CATTGCTGATAGACAAAAAC+TGG	-	Chr8:77748385-77748404	None:intergenic	35.0%
	CTTGACATTTCAAAGCAACT+AGG	-	Chr8:77748110-77748129	None:intergenic	35.0%
	GCTATGCATAACAAATAGCA+GGG	-	Chr8:77747924-77747943	None:intergenic	35.0%
	GCTGAGGATAAGGTATATTA+GGG	+	Chr8:77747690-77747709	MsG0880046760.01.T01:intron	35.0%
	TCACATCAAAACAAGCACTT+GGG	+	Chr8:77748471-77748490	MsG0880046760.01.T01:CDS	35.0%
	TCAGCTAACAATCACCATAA+TGG	+	Chr8:77747432-77747451	MsG0880046760.01.T01:CDS	35.0%
	TGCTATGCATAACAAATAGC+AGG	-	Chr8:77747925-77747944	None:intergenic	35.0%
	TGCTGAGGATAAGGTATATT+AGG	+	Chr8:77747689-77747708	MsG0880046760.01.T01:intron	35.0%
	TGTTCTGTGACAACTAATGA+AGG	+	Chr8:77748527-77748546	MsG0880046760.01.T01:CDS	35.0%
	TTCAGAAATACCCCTATTCA+AGG	-	Chr8:77747610-77747629	None:intergenic	35.0%
	TTTGATGTGACGAACACATA+GGG	-	Chr8:77748461-77748480	None:intergenic	35.0%
!	TGAATATTTGGTTGGTATGC+AGG	+	Chr8:77748668-77748687	MsG0880046760.01.T01:intron	35.0%
!	TTTTGATGTGACGAACACAT+AGG	-	Chr8:77748462-77748481	None:intergenic	35.0%
!!	CTAGTTGCTTTGAAATGTCA+AGG	+	Chr8:77748108-77748127	MsG0880046760.01.T01:intron	35.0%
	ACAACATTCAGGGGAAAGAT+AGG	-	Chr8:77748160-77748179	None:intergenic	40.0%
	AGATGGAGAAGAATCTCAAG+AGG	-	Chr8:77747660-77747679	None:intergenic	40.0%
	ATCGAGACCTAACAACATTC+AGG	-	Chr8:77748171-77748190	None:intergenic	40.0%
	CAGCTACTAAGACCTTGAAT+AGG	+	Chr8:77747595-77747614	MsG0880046760.01.T01:intron	40.0%
	GCTACTAAGACCTTGAATAG+GGG	+	Chr8:77747597-77747616	MsG0880046760.01.T01:intron	40.0%
	GTCACATCAAAACAAGCACT+TGG	+	Chr8:77748470-77748489	MsG0880046760.01.T01:CDS	40.0%
	TCGAGACCTAACAACATTCA+GGG	-	Chr8:77748170-77748189	None:intergenic	40.0%
	TCTTTCCCCTGAATGTTGTT+AGG	+	Chr8:77748161-77748180	MsG0880046760.01.T01:intron	40.0%
!!	CTCAAAAAGGGTGCTAATGA+AGG	+	Chr8:77747487-77747506	MsG0880046760.01.T01:CDS	40.0%
	ACCTTATCCTCAGCAAGTAG+AGG	-	Chr8:77747684-77747703	None:intergenic	45.0%
	AGAACAAGCAATCACTGGTC+TGG	-	Chr8:77748513-77748532	None:intergenic	45.0%
	AGGAGAAGCAGTTAACCCTA+AGG	+	Chr8:77747390-77747409	MsG0880046760.01.T01:CDS	45.0%
	CCACAACCCAAACATCTGTA+TGG	-	Chr8:77747972-77747991	None:intergenic	45.0%
	CGAGACCTAACAACATTCAG+GGG	-	Chr8:77748169-77748188	None:intergenic	45.0%
	GTCACAGAACAAGCAATCAC+TGG	-	Chr8:77748518-77748537	None:intergenic	45.0%
	GTCTCAGATTCAGCAACTCA+AGG	+	Chr8:77748562-77748581	MsG0880046760.01.T01:CDS	45.0%
	TAACCCTAAGGCATACCCTT+TGG	+	Chr8:77747402-77747421	MsG0880046760.01.T01:CDS	45.0%
	TTAGCTGAGCATCAGCCAAA+GGG	-	Chr8:77747420-77747439	None:intergenic	45.0%
!	TCCTCTACTTGCTGAGGATA+AGG	+	Chr8:77747680-77747699	MsG0880046760.01.T01:CDS	45.0%
!!	CCATACAGATGTTTGGGTTG+TGG	+	Chr8:77747969-77747988	MsG0880046760.01.T01:intron	45.0%
	CCTCAGCAAGTAGAGGAAGA+TGG	-	Chr8:77747677-77747696	None:intergenic	50.0%
	GTTAGCTGAGCATCAGCCAA+AGG	-	Chr8:77747421-77747440	None:intergenic	50.0%
!	CAGCCAAAGGGTATGCCTTA+GGG	-	Chr8:77747408-77747427	None:intergenic	50.0%
!	CCATCTTCCTCTACTTGCTG+AGG	+	Chr8:77747674-77747693	MsG0880046760.01.T01:CDS	50.0%
!	TCAGCCAAAGGGTATGCCTT+AGG	-	Chr8:77747409-77747428	None:intergenic	50.0%
	CAAGCACTTGGGCGTGCTTG+TGG	+	Chr8:77748482-77748501	MsG0880046760.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	77747373	77748716	77747373	ID=MsG0880046760.01;Name=MsG0880046760.01
Chr8	mRNA	77747373	77748716	77747373	ID=MsG0880046760.01.T01;Parent=MsG0880046760.01;Name=MsG0880046760.01.T01;_AED=0.50;_eAED=0.52;_QI=0\|0\|0\|1\|0.66\|0.75\|4\|0\|132
Chr8	exon	77747373	77747508	77747373	ID=MsG0880046760.01.T01:exon:33249;Parent=MsG0880046760.01.T01
Chr8	exon	77747598	77747701	77747598	ID=MsG0880046760.01.T01:exon:33250;Parent=MsG0880046760.01.T01
Chr8	exon	77748452	77748583	77748452	ID=MsG0880046760.01.T01:exon:33251;Parent=MsG0880046760.01.T01
Chr8	exon	77748690	77748716	77748690	ID=MsG0880046760.01.T01:exon:33252;Parent=MsG0880046760.01.T01
Chr8	CDS	77747373	77747508	77747373	ID=MsG0880046760.01.T01:cds;Parent=MsG0880046760.01.T01
Chr8	CDS	77747598	77747701	77747598	ID=MsG0880046760.01.T01:cds;Parent=MsG0880046760.01.T01
Chr8	CDS	77748452	77748583	77748452	ID=MsG0880046760.01.T01:cds;Parent=MsG0880046760.01.T01
Chr8	CDS	77748690	77748716	77748690	ID=MsG0880046760.01.T01:cds;Parent=MsG0880046760.01.T01

Gene Sequence

>MsG0880046760.01.T01

ATGAAAATTTTGCAGACAGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCTCTACTTGCTGAGGATAAGAATGTTCCCTATGTGTTCGTCACATCAAAACAAGCACTTGGGCGTGCTTGTGGTGTCACCAGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGATGCTATTGAGAAGCTGCTAATCTGA

Protein sequence

>MsG0880046760.01.T01

MKILQTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI*