AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029157.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029157.01.T02 MTR_5g082000 88.673 309 10 1 1 309 1 284 0.0 550
MsG0580029157.01.T02 MTR_3g070190 80.192 313 33 2 1 309 1 288 5.94e-176 488
MsG0580029157.01.T02 MTR_3g070180 29.276 304 179 7 10 303 8 285 1.29e-29 115
MsG0580029157.01.T02 MTR_5g081980 29.010 293 172 7 21 303 19 285 9.39e-29 112
MsG0580029157.01.T02 MTR_5g082010 70.000 40 12 0 203 242 55 94 2.07e-13 65.9
MsG0580029157.01.T01 MTR_5g082000 88.673 309 10 1 1 309 1 284 0.0 550
MsG0580029157.01.T01 MTR_3g070190 80.192 313 33 2 1 309 1 288 5.94e-176 488
MsG0580029157.01.T01 MTR_3g070180 29.276 304 179 7 10 303 8 285 1.29e-29 115
MsG0580029157.01.T01 MTR_5g081980 29.010 293 172 7 21 303 19 285 9.39e-29 112
MsG0580029157.01.T01 MTR_5g082010 70.000 40 12 0 203 242 55 94 2.07e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029157.01.T02 AT2G39990 65.190 316 77 2 2 309 3 293 2.69e-141 400
MsG0580029157.01.T02 AT3G11270 29.568 301 179 7 10 303 8 282 3.67e-31 119
MsG0580029157.01.T02 AT3G11270 29.276 304 179 7 10 303 8 285 1.02e-30 117
MsG0580029157.01.T02 AT5G05780 28.239 301 183 7 10 303 8 282 1.00e-28 112
MsG0580029157.01.T02 AT5G05780 27.961 304 183 7 10 303 8 285 3.13e-28 111
MsG0580029157.01.T01 AT2G39990 65.190 316 77 2 2 309 3 293 2.69e-141 400
MsG0580029157.01.T01 AT3G11270 29.568 301 179 7 10 303 8 282 3.67e-31 119
MsG0580029157.01.T01 AT3G11270 29.276 304 179 7 10 303 8 285 1.02e-30 117
MsG0580029157.01.T01 AT5G05780 28.239 301 183 7 10 303 8 282 1.00e-28 112
MsG0580029157.01.T01 AT5G05780 27.961 304 183 7 10 303 8 285 3.13e-28 111

Find 23 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTTACTTGGAGAATATT+TGG 0.198882 5:+93019668 None:intergenic
GGTACTGGTTTGTGATATTA+TGG 0.306877 5:+93019693 None:intergenic
ACCTCTTGTGGTACAAATCT+TGG 0.323744 5:+93019848 None:intergenic
TATGGCACCTATTTGACTCT+TGG 0.364236 5:+93019711 None:intergenic
TGTGGTTCCAAGAGTCAAAT+AGG 0.388573 5:-93019718 MsG0580029157.01.T01:intron
TGTACCACAAGAGGTCTAAT+AGG 0.404557 5:-93019840 MsG0580029157.01.T01:three_prime_UTR
GGAGAATATTTGGCTGGTAC+TGG 0.411575 5:+93019678 None:intergenic
GAACAGAGGGAGTCTTTACT+TGG 0.417080 5:+93019657 None:intergenic
TCGTTTGATAATCAATATCA+AGG 0.435439 5:+93019745 None:intergenic
GTCATATCTTTGAAATAAAA+CGG 0.444829 5:-93019882 MsG0580029157.01.T01:three_prime_UTR
TTGAAATAAAACGGAGAATG+AGG 0.452781 5:-93019873 MsG0580029157.01.T01:three_prime_UTR
AGCAAAGAGGAATTATATAT+AGG 0.457872 5:+93019635 None:intergenic
TTACTTGGAGAATATTTGGC+TGG 0.495106 5:+93019672 None:intergenic
CACAAGGACACAGCTCAGCC+TGG 0.504225 5:-93020122 MsG0580029157.01.T01:CDS
GAATTATATATAGGAACAGA+GGG 0.519077 5:+93019644 None:intergenic
TGAGGTCTCTTTGTAGTGAG+TGG 0.520410 5:-93019928 MsG0580029157.01.T01:three_prime_UTR
GGAATTATATATAGGAACAG+AGG 0.529003 5:+93019643 None:intergenic
TATTGAGAAATATCACAAAA+CGG 0.546724 5:+93020025 None:intergenic
TCCAAGATTTGTACCACAAG+AGG 0.575199 5:-93019849 MsG0580029157.01.T01:three_prime_UTR
AGCACCTATTAGACCTCTTG+TGG 0.577301 5:+93019836 None:intergenic
TGATTATCAAACGAAGAATG+TGG 0.619310 5:-93019736 MsG0580029157.01.T01:three_prime_UTR
CAATCACAGAATCTGAGCAG+CGG 0.707909 5:+93020084 None:intergenic
TATATTTATCTAGCATCACA+AGG 0.712944 5:-93020138 MsG0580029157.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTTAATAAATTGTAG+GGG - Chr5:93023078-93023097 MsG0580029157.01.T01:intron 10.0%
!! GAATAAATTTAATAAATTGT+AGG - Chr5:93023076-93023095 MsG0580029157.01.T01:intron 10.0%
!!! TCTAATTTAAAATGAAAATT+TGG - Chr5:93020331-93020350 MsG0580029157.01.T01:intron 10.0%
!!! TTTCATTTACTTTTATTAAT+AGG - Chr5:93020748-93020767 MsG0580029157.01.T01:intron 10.0%
!! AAATGGCTTATAAATTATAA+TGG - Chr5:93022704-93022723 MsG0580029157.01.T01:intron 15.0%
!! TAGATTGATATACATATATT+TGG - Chr5:93020555-93020574 MsG0580029157.01.T01:CDS 15.0%
!! TGATTATCTATAAATACAAA+AGG - Chr5:93020704-93020723 MsG0580029157.01.T01:intron 15.0%
!!! AATTATGATAATAGTTCTTT+TGG - Chr5:93023118-93023137 MsG0580029157.01.T01:intron 15.0%
!!! ATCATCTTTAAAATAAAACA+AGG + Chr5:93022429-93022448 None:intergenic 15.0%
!!! CTTGTTTTGTAAAAAAAATT+TGG - Chr5:93020870-93020889 MsG0580029157.01.T01:intron 15.0%
!! AAGAAATATCCTAAAATATG+TGG + Chr5:93022282-93022301 None:intergenic 20.0%
!! AGAAAGAAATAAAACTGTAT+AGG - Chr5:93020620-93020639 MsG0580029157.01.T01:intron 20.0%
!! AGAAATATCCTAAAATATGT+GGG + Chr5:93022281-93022300 None:intergenic 20.0%
!! ATTTATGTATCACTTTATCA+TGG - Chr5:93022636-93022655 MsG0580029157.01.T01:intron 20.0%
!! TAAAACAAGGAAGAAAAAAA+AGG + Chr5:93022416-93022435 None:intergenic 20.0%
!! TATTGAGAAATATCACAAAA+CGG + Chr5:93023359-93023378 None:intergenic 20.0%
!! TTAGAATAAAATTAGGTGTT+TGG - Chr5:93022317-93022336 MsG0580029157.01.T01:CDS 20.0%
!!! AACTTGATGCATTTTTTAAT+TGG + Chr5:93020479-93020498 None:intergenic 20.0%
!!! AATAAATGCAATTTTGCTTA+GGG - Chr5:93023327-93023346 MsG0580029157.01.T01:intron 20.0%
!!! AGATTTTTAGAAGAAACAAA+TGG + Chr5:93020660-93020679 None:intergenic 20.0%
!!! AGGTAATTTCATATTTTCTT+TGG - Chr5:93023428-93023447 MsG0580029157.01.T01:intron 20.0%
!!! AGTTGCTTTAGAATAAAATT+AGG - Chr5:93022310-93022329 MsG0580029157.01.T01:CDS 20.0%
!!! CTGTTTTATGTTTTTATCAA+AGG - Chr5:93021212-93021231 MsG0580029157.01.T01:intron 20.0%
!!! GTCATATCTTTGAAATAAAA+CGG - Chr5:93023499-93023518 MsG0580029157.01.T01:intron 20.0%
!!! TAAGGAAAATGTTAAGTTTT+GGG - Chr5:93020143-93020162 MsG0580029157.01.T01:CDS 20.0%
!!! TATAATTTGTAGCTTTGAAA+TGG - Chr5:93022687-93022706 MsG0580029157.01.T01:intron 20.0%
!!! TTAAAATGAAAATTTGGTGT+TGG - Chr5:93020337-93020356 MsG0580029157.01.T01:intron 20.0%
!!! TTAAGGAAAATGTTAAGTTT+TGG - Chr5:93020142-93020161 MsG0580029157.01.T01:CDS 20.0%
!!! TTCACTTAGTGATTTTTTTA+TGG - Chr5:93021503-93021522 MsG0580029157.01.T01:intron 20.0%
!!! TTGCAGGTAATTTTAATTTT+GGG - Chr5:93023023-93023042 MsG0580029157.01.T01:intron 20.0%
!!! TTTTGTGATATTTCTCAATA+TGG - Chr5:93023359-93023378 MsG0580029157.01.T01:intron 20.0%
! AAATGTATATCAGATGTTGT+AGG - Chr5:93022948-93022967 MsG0580029157.01.T01:intron 25.0%
! AGCAAAGAGGAATTATATAT+AGG + Chr5:93023749-93023768 None:intergenic 25.0%
! AGTAAAGTATATGGATATCA+AGG + Chr5:93021441-93021460 None:intergenic 25.0%
! ATTTACAGAAAGAATGATAC+TGG - Chr5:93021580-93021599 MsG0580029157.01.T01:intron 25.0%
! CATATACTTTACTTAAATCC+AGG - Chr5:93021448-93021467 MsG0580029157.01.T01:intron 25.0%
! CATGAAAAGTAAAACTTTGA+TGG - Chr5:93022105-93022124 MsG0580029157.01.T01:intron 25.0%
! CTGGATTTAAGTAAAGTATA+TGG + Chr5:93021450-93021469 None:intergenic 25.0%
! CTTTATAGAAGAAATGTCTT+GGG - Chr5:93022501-93022520 MsG0580029157.01.T01:intron 25.0%
! GAATTATATATAGGAACAGA+GGG + Chr5:93023740-93023759 None:intergenic 25.0%
! GTTTCAAACAATTTATCACT+TGG - Chr5:93021080-93021099 MsG0580029157.01.T01:intron 25.0%
! TATATTTATCTAGCATCACA+AGG - Chr5:93023243-93023262 MsG0580029157.01.T01:intron 25.0%
! TATCCATATACTTTGCTTAA+AGG - Chr5:93021692-93021711 MsG0580029157.01.T01:intron 25.0%
! TTATACTGATACAACACTAA+TGG + Chr5:93022672-93022691 None:intergenic 25.0%
! TTCAAATTTCGCTATGTTAT+AGG + Chr5:93020020-93020039 None:intergenic 25.0%
!! AAATGCATCAAGTTTAAGTT+TGG - Chr5:93020485-93020504 MsG0580029157.01.T01:CDS 25.0%
!! AAGGAAAATGTTAAGTTTTG+GGG - Chr5:93020144-93020163 MsG0580029157.01.T01:CDS 25.0%
!! CTTTTATTAATAGGTTGCTT+TGG - Chr5:93020757-93020776 MsG0580029157.01.T01:intron 25.0%
!! GAATAAATGCAATTTTGCTT+AGG - Chr5:93023326-93023345 MsG0580029157.01.T01:intron 25.0%
!! TCGTTTGATAATCAATATCA+AGG + Chr5:93023639-93023658 None:intergenic 25.0%
!! TTCATATTTTCTTTGGTATG+AGG - Chr5:93023435-93023454 MsG0580029157.01.T01:intron 25.0%
!! TTTTGTATTTCTTCACTTGT+TGG - Chr5:93021748-93021767 MsG0580029157.01.T01:intron 25.0%
!!! CTTGCAGGTAATTTTAATTT+TGG - Chr5:93023022-93023041 MsG0580029157.01.T01:intron 25.0%
!!! GTTTGAAACATATGCTTTTA+TGG + Chr5:93021069-93021088 None:intergenic 25.0%
!!! TGCAGGTAATTTTAATTTTG+GGG - Chr5:93023024-93023043 MsG0580029157.01.T01:intron 25.0%
!!! TTTTTCCTGATTGAAGTATT+AGG - Chr5:93021380-93021399 MsG0580029157.01.T01:intron 25.0%
AAAATCCCATCTGATTTAGA+AGG - Chr5:93022822-93022841 MsG0580029157.01.T01:intron 30.0%
AAACAAATGCAATCTCAGAA+AGG + Chr5:93019878-93019897 None:intergenic 30.0%
AAATCCCATCTGATTTAGAA+GGG - Chr5:93022823-93022842 MsG0580029157.01.T01:intron 30.0%
AACATCATTGACGTATTTGT+AGG + Chr5:93022892-93022911 None:intergenic 30.0%
ACAGTTTCAAGAAATTCCTT+TGG - Chr5:93021121-93021140 MsG0580029157.01.T01:intron 30.0%
ACATCGTCAATGTTAAGTTT+GGG - Chr5:93020378-93020397 MsG0580029157.01.T01:CDS 30.0%
ACTGTATAGGTATTTGATGT+TGG - Chr5:93020633-93020652 MsG0580029157.01.T01:intron 30.0%
AGACCTTTAAGCAAAGTATA+TGG + Chr5:93021698-93021717 None:intergenic 30.0%
ATCACACATCGATCTATTAA+AGG + Chr5:93021332-93021351 None:intergenic 30.0%
ATCATCATGAGTAACATGTT+TGG - Chr5:93022133-93022152 MsG0580029157.01.T01:intron 30.0%
ATGATTCGTTTATTTGCCTT+TGG - Chr5:93022186-93022205 MsG0580029157.01.T01:intron 30.0%
ATGCAATGTTTGGTACAATT+TGG - Chr5:93022234-93022253 MsG0580029157.01.T01:CDS 30.0%
ATGTATCTATTAGTTGAGAG+TGG - Chr5:93022535-93022554 MsG0580029157.01.T01:intron 30.0%
CAAAACAAGCTTAAAAGAAC+TGG + Chr5:93020859-93020878 None:intergenic 30.0%
CATATCGTGATAAAAATGTG+GGG + Chr5:93022217-93022236 None:intergenic 30.0%
CCAAGACATTTCTTCTATAA+AGG + Chr5:93022503-93022522 None:intergenic 30.0%
CCTTTATAGAAGAAATGTCT+TGG - Chr5:93022500-93022519 MsG0580029157.01.T01:intron 30.0%
CTTCAGATTATCAACTGTAA+AGG + Chr5:93022787-93022806 None:intergenic 30.0%
CTTCTCGAGAATAAAATTCA+TGG + Chr5:93020992-93021011 None:intergenic 30.0%
GACGAGTTTAGAAAAATCAT+GGG + Chr5:93020455-93020474 None:intergenic 30.0%
GCATATCGTGATAAAAATGT+GGG + Chr5:93022218-93022237 None:intergenic 30.0%
GGAACTGTACATATTTCTTA+AGG - Chr5:93020125-93020144 MsG0580029157.01.T01:CDS 30.0%
GGAATTATATATAGGAACAG+AGG + Chr5:93023741-93023760 None:intergenic 30.0%
GTCTTTACTTGGAGAATATT+TGG + Chr5:93023716-93023735 None:intergenic 30.0%
TACATCGTCAATGTTAAGTT+TGG - Chr5:93020377-93020396 MsG0580029157.01.T01:CDS 30.0%
TATGAAGCAATGTTGTTGAA+TGG - Chr5:93019983-93020002 MsG0580029157.01.T01:three_prime_UTR 30.0%
TATGTCCTAATACTTCAATC+AGG + Chr5:93021388-93021407 None:intergenic 30.0%
TCATCATGAGTAACATGTTT+GGG - Chr5:93022134-93022153 MsG0580029157.01.T01:intron 30.0%
TGACGAGTTTAGAAAAATCA+TGG + Chr5:93020456-93020475 None:intergenic 30.0%
TGATTATCAAACGAAGAATG+TGG - Chr5:93023645-93023664 MsG0580029157.01.T01:CDS 30.0%
TGCATATCGTGATAAAAATG+TGG + Chr5:93022219-93022238 None:intergenic 30.0%
TGTCAACAGTCAAATGTATT+GGG + Chr5:93021022-93021041 None:intergenic 30.0%
TTATCACGATATGCAATGTT+TGG - Chr5:93022224-93022243 MsG0580029157.01.T01:intron 30.0%
TTGAAATAAAACGGAGAATG+AGG - Chr5:93023508-93023527 MsG0580029157.01.T01:intron 30.0%
TTGCTTAAAGGTCTAAATCT+AGG - Chr5:93021704-93021723 MsG0580029157.01.T01:intron 30.0%
! ATTTTCCTTTCCTTGTGAAA+TGG - Chr5:93021981-93022000 MsG0580029157.01.T01:intron 30.0%
! GTATAAGTCCCACATATTTT+AGG - Chr5:93022270-93022289 MsG0580029157.01.T01:CDS 30.0%
! TAGCTAGTGTCATTGTTATT+AGG - Chr5:93021628-93021647 MsG0580029157.01.T01:intron 30.0%
! TCTGTAGATTAATGCGATTT+TGG - Chr5:93020171-93020190 MsG0580029157.01.T01:intron 30.0%
! TTTGAGAACACATTTTCTCA+GGG - Chr5:93023169-93023188 MsG0580029157.01.T01:CDS 30.0%
! TTTTCCTTTCCTTGTGAAAT+GGG - Chr5:93021982-93022001 MsG0580029157.01.T01:intron 30.0%
!! ATTTCCCAATTTTGTTGTCT+GGG + Chr5:93022932-93022951 None:intergenic 30.0%
!! TGGAGTTCAAAATGCTATTT+TGG - Chr5:93020208-93020227 MsG0580029157.01.T01:intron 30.0%
!!! ATTTTGGGGTTATATTGTAG+TGG - Chr5:93023038-93023057 MsG0580029157.01.T01:intron 30.0%
!!! CTTCACTTGTTGGTTTTTTA+TGG - Chr5:93021758-93021777 MsG0580029157.01.T01:intron 30.0%
!!! TGGCAGATTACTACTTTTTT+TGG - Chr5:93020408-93020427 MsG0580029157.01.T01:CDS 30.0%
AACAGTCAAATGTATTGGGT+TGG + Chr5:93021018-93021037 None:intergenic 35.0%
AACATAGCAAGCTGTTCTTA+CGG + Chr5:93023144-93023163 None:intergenic 35.0%
AAGACAAAAACAATGACCCT+TGG + Chr5:93021884-93021903 None:intergenic 35.0%
AATTCCTTTGGATCTTCGTA+TGG - Chr5:93021133-93021152 MsG0580029157.01.T01:intron 35.0%
AGCATCAATGACACATCTAT+TGG - Chr5:93022851-93022870 MsG0580029157.01.T01:intron 35.0%
ATACATTTGACTGTTGACAC+AGG - Chr5:93021023-93021042 MsG0580029157.01.T01:intron 35.0%
ATCGTCAATGTTAAGTTTGG+GGG - Chr5:93020380-93020399 MsG0580029157.01.T01:CDS 35.0%
ATCTGTGTCTCGTTTGTATT+TGG - Chr5:93021943-93021962 MsG0580029157.01.T01:intron 35.0%
ATTGACTGTGTGTTAACTTC+TGG - Chr5:93022761-93022780 MsG0580029157.01.T01:intron 35.0%
ATTTGAATTAGCAGCGAGTA+TGG + Chr5:93020286-93020305 None:intergenic 35.0%
CAAATACGTCAATGATGTTG+TGG - Chr5:93022893-93022912 MsG0580029157.01.T01:intron 35.0%
CACAAAGTGTATTTGCAATC+AGG - Chr5:93022045-93022064 MsG0580029157.01.T01:intron 35.0%
CATCGTCAATGTTAAGTTTG+GGG - Chr5:93020379-93020398 MsG0580029157.01.T01:CDS 35.0%
GAGAGTAAAAACCTGATCAA+CGG + Chr5:93019843-93019862 None:intergenic 35.0%
GGGATAGTAACATATTATGG+TGG + Chr5:93020793-93020812 None:intergenic 35.0%
GGTGGGATAGTAACATATTA+TGG + Chr5:93020796-93020815 None:intergenic 35.0%
GTATGAATTACGGATATCGA+CGG + Chr5:93019801-93019820 None:intergenic 35.0%
GTCATTCACAATATAGCCAA+GGG - Chr5:93021865-93021884 MsG0580029157.01.T01:intron 35.0%
GTCTTACTCTTAAAACCATC+TGG + Chr5:93022346-93022365 None:intergenic 35.0%
GTGTCAACAGTCAAATGTAT+TGG + Chr5:93021023-93021042 None:intergenic 35.0%
GTGTGATATTGTACTAGGTT+AGG - Chr5:93021345-93021364 MsG0580029157.01.T01:intron 35.0%
GTTAGATTAACCACAGATTG+TGG + Chr5:93020098-93020117 None:intergenic 35.0%
TACAGAAAGAATGATACCTG+AGG - Chr5:93021838-93021857 MsG0580029157.01.T01:intron 35.0%
TCGATGTGTGATATTGTACT+AGG - Chr5:93021340-93021359 MsG0580029157.01.T01:intron 35.0%
TCGTTTGTATTTGGGAAAAC+TGG - Chr5:93021952-93021971 MsG0580029157.01.T01:intron 35.0%
TCTGTGGTTAATCTAACAGA+TGG - Chr5:93020101-93020120 MsG0580029157.01.T01:CDS 35.0%
TCTGTGTCTCGTTTGTATTT+GGG - Chr5:93021944-93021963 MsG0580029157.01.T01:intron 35.0%
TGCTTGCTTTCATCAATTCA+TGG - Chr5:93021274-93021293 MsG0580029157.01.T01:intron 35.0%
TGTCATTCACAATATAGCCA+AGG - Chr5:93021864-93021883 MsG0580029157.01.T01:intron 35.0%
TGTTAAGTTTGGGGGAATTA+TGG - Chr5:93020388-93020407 MsG0580029157.01.T01:CDS 35.0%
TTACTCTTAAAACCATCTGG+AGG + Chr5:93022343-93022362 None:intergenic 35.0%
TTACTTGGAGAATATTTGGC+TGG + Chr5:93023712-93023731 None:intergenic 35.0%
TTCGTTTATTTGCCTTTGGA+AGG - Chr5:93022190-93022209 MsG0580029157.01.T01:intron 35.0%
TTGACCCAGACAACAAAATT+GGG - Chr5:93022925-93022944 MsG0580029157.01.T01:intron 35.0%
TTTGACTCAGGTTCATAGAT+CGG - Chr5:93022157-93022176 MsG0580029157.01.T01:intron 35.0%
! AACACATTTTCTCAGGGTTT+TGG - Chr5:93023175-93023194 MsG0580029157.01.T01:CDS 35.0%
! ACACATTTTCTCAGGGTTTT+GGG - Chr5:93023176-93023195 MsG0580029157.01.T01:CDS 35.0%
! ATTGAGTTTTTACTTCGTGC+AGG - Chr5:93020925-93020944 MsG0580029157.01.T01:intron 35.0%
! CATTGGATGTACGTATTGAT+TGG - Chr5:93021169-93021188 MsG0580029157.01.T01:intron 35.0%
! GTTTGAGAACACATTTTCTC+AGG - Chr5:93023168-93023187 MsG0580029157.01.T01:CDS 35.0%
!! CAATGCACTTTTTATTGCGT+AGG - Chr5:93023408-93023427 MsG0580029157.01.T01:intron 35.0%
!! CAGCTGCAAGTTGAATATTT+TGG - Chr5:93022019-93022038 MsG0580029157.01.T01:intron 35.0%
!! CATATATTTGGTGTAGCATG+TGG - Chr5:93020567-93020586 MsG0580029157.01.T01:CDS 35.0%
!! CATTTCCCAATTTTGTTGTC+TGG + Chr5:93022933-93022952 None:intergenic 35.0%
!! CTCTTTTAAAGGGGTTTTGA+GGG - Chr5:93022373-93022392 MsG0580029157.01.T01:CDS 35.0%
!! GGTACTGGTTTGTGATATTA+TGG + Chr5:93023691-93023710 None:intergenic 35.0%
!! TCTCTTTTAAAGGGGTTTTG+AGG - Chr5:93022372-93022391 MsG0580029157.01.T01:CDS 35.0%
!! TGAAAATTTGGTGTTGGATG+TGG - Chr5:93020343-93020362 MsG0580029157.01.T01:intron 35.0%
!! TTGGTACAATTTGGTGCATT+GGG - Chr5:93022243-93022262 MsG0580029157.01.T01:CDS 35.0%
!! TTTGGTACAATTTGGTGCAT+TGG - Chr5:93022242-93022261 MsG0580029157.01.T01:CDS 35.0%
!! TTTTCAGGATCATTTGCTCA+TGG - Chr5:93023220-93023239 MsG0580029157.01.T01:intron 35.0%
!!! AAGAGTGTTTCATGGTTTTC+AGG - Chr5:93023205-93023224 MsG0580029157.01.T01:CDS 35.0%
!!! TCAGTTTTTGGTCATGATCA+AGG + Chr5:93021924-93021943 None:intergenic 35.0%
AAATTCCGCTAGCTATGTCA+CGG + Chr5:93020058-93020077 None:intergenic 40.0%
AACCATGAAACACTCTTGTC+AGG + Chr5:93023202-93023221 None:intergenic 40.0%
AAGAAATGTCTTGGGTCTAC+TGG - Chr5:93022509-93022528 MsG0580029157.01.T01:intron 40.0%
AAGTCCCATTTCACAAGGAA+AGG + Chr5:93021989-93022008 None:intergenic 40.0%
AATATTCAACTTGCAGCTGC+AGG + Chr5:93022018-93022037 None:intergenic 40.0%
AATCCCAAGGAAGCTATAGT+TGG - Chr5:93020824-93020843 MsG0580029157.01.T01:intron 40.0%
ACACAGAAGAGAATACAGAG+AGG + Chr5:93022467-93022486 None:intergenic 40.0%
ACCTCTTGTGGTACAAATCT+TGG + Chr5:93023536-93023555 None:intergenic 40.0%
ACGTCAATGATGTTGTGGAA+GGG - Chr5:93022898-93022917 MsG0580029157.01.T01:intron 40.0%
AGAAATGTCTTGGGTCTACT+GGG - Chr5:93022510-93022529 MsG0580029157.01.T01:intron 40.0%
ATCTCAGAAAGGAATGCATG+TGG + Chr5:93019867-93019886 None:intergenic 40.0%
ATTGTGAATGACATTGCCTC+AGG + Chr5:93021857-93021876 None:intergenic 40.0%
CAGAAAGAATGATACTGGAC+TGG - Chr5:93021585-93021604 MsG0580029157.01.T01:intron 40.0%
CAGATGTTGAAGATAACGAG+AGG + Chr5:93019701-93019720 None:intergenic 40.0%
CATCCAACTATAGCTTCCTT+GGG + Chr5:93020830-93020849 None:intergenic 40.0%
CCATCCCTTCTAAATCAGAT+GGG + Chr5:93022830-93022849 None:intergenic 40.0%
GAAGAGAATACAGAGAGGAA+AGG + Chr5:93022462-93022481 None:intergenic 40.0%
GAAGCAATGTTGTTGAATGG+CGG - Chr5:93019986-93020005 MsG0580029157.01.T01:three_prime_UTR 40.0%
GTGGTTAATCTAACAGATGG+CGG - Chr5:93020104-93020123 MsG0580029157.01.T01:CDS 40.0%
GTTATTAGGACAGAGTGACT+TGG - Chr5:93021642-93021661 MsG0580029157.01.T01:intron 40.0%
GTTGACCCAGACAACAAAAT+TGG - Chr5:93022924-93022943 MsG0580029157.01.T01:intron 40.0%
TACGTCAATGATGTTGTGGA+AGG - Chr5:93022897-93022916 MsG0580029157.01.T01:intron 40.0%
TATGGCACCTATTTGACTCT+TGG + Chr5:93023673-93023692 None:intergenic 40.0%
TCAACCATACGAAGATCCAA+AGG + Chr5:93021140-93021159 None:intergenic 40.0%
TCCAAGATTTGTACCACAAG+AGG - Chr5:93023532-93023551 MsG0580029157.01.T01:intron 40.0%
TCCATCCCTTCTAAATCAGA+TGG + Chr5:93022831-93022850 None:intergenic 40.0%
TCTTCTTCCCAAACCTTATC+CGG - Chr5:93019665-93019684 MsG0580029157.01.T01:three_prime_UTR 40.0%
TGTACCACAAGAGGTCTAAT+AGG - Chr5:93023541-93023560 MsG0580029157.01.T01:intron 40.0%
TGTGGTTCCAAGAGTCAAAT+AGG - Chr5:93023663-93023682 MsG0580029157.01.T01:CDS 40.0%
TTGACTCAGGTTCATAGATC+GGG - Chr5:93022158-93022177 MsG0580029157.01.T01:intron 40.0%
TTTCCTTGTGAAATGGGACT+TGG - Chr5:93021988-93022007 MsG0580029157.01.T01:intron 40.0%
! AAAGAATGATACTGGACTGG+AGG - Chr5:93021588-93021607 MsG0580029157.01.T01:intron 40.0%
! AACATGTTTGGGTTTGACTC+AGG - Chr5:93022145-93022164 MsG0580029157.01.T01:intron 40.0%
! AATGACACATCTATTGGCCT+TGG - Chr5:93022857-93022876 MsG0580029157.01.T01:intron 40.0%
! ACGAGACACAGATCAGTTTT+TGG + Chr5:93021936-93021955 None:intergenic 40.0%
! GCATTTTGAACTCCATAGTG+TGG + Chr5:93020203-93020222 None:intergenic 40.0%
! TGATAATGCAAAAGAGAGCC+TGG + Chr5:93021469-93021488 None:intergenic 40.0%
! TTTGTAGGTGTCATCAACCA+AGG + Chr5:93022877-93022896 None:intergenic 40.0%
! TTTTGGCAAAAGCCACACTA+TGG - Chr5:93020188-93020207 MsG0580029157.01.T01:intron 40.0%
!! CTTCCTTGGGATTCACTTTT+TGG + Chr5:93020817-93020836 None:intergenic 40.0%
!!! CTGCTATTTTCCACAATCTG+TGG - Chr5:93020085-93020104 MsG0580029157.01.T01:exon 40.0%
!!! CTTGGGATTCACTTTTTGGT+GGG + Chr5:93020813-93020832 None:intergenic 40.0%
!!! TTAAAGGGGTTTTGAGGGAT+TGG - Chr5:93022378-93022397 MsG0580029157.01.T01:CDS 40.0%
AAAATGTGGGGACCTTCCAA+AGG + Chr5:93022205-93022224 None:intergenic 45.0%
AACCTTCCCGGATAAGGTTT+GGG + Chr5:93019675-93019694 None:intergenic 45.0%
AAGCTTATCAAATGCCGAGG+AGG + Chr5:93022991-93023010 None:intergenic 45.0%
ACAGGATTTGCAGATGGAGA+GGG - Chr5:93021041-93021060 MsG0580029157.01.T01:intron 45.0%
ACTTCGTGCAGGTATTCAAC+TGG - Chr5:93020936-93020955 MsG0580029157.01.T01:intron 45.0%
AGCACCTATTAGACCTCTTG+TGG + Chr5:93023548-93023567 None:intergenic 45.0%
AGCTTATCAAATGCCGAGGA+GGG + Chr5:93022990-93023009 None:intergenic 45.0%
CAATCACAGAATCTGAGCAG+CGG + Chr5:93023300-93023319 None:intergenic 45.0%
CACAAGCTTATCAAATGCCG+AGG + Chr5:93022994-93023013 None:intergenic 45.0%
CCAAGGAAGCTATAGTTGGA+TGG - Chr5:93020828-93020847 MsG0580029157.01.T01:intron 45.0%
CCACCAAAAAGTGAATCCCA+AGG - Chr5:93020811-93020830 MsG0580029157.01.T01:intron 45.0%
CCATCCAACTATAGCTTCCT+TGG + Chr5:93020831-93020850 None:intergenic 45.0%
CCCATCTGATTTAGAAGGGA+TGG - Chr5:93022827-93022846 MsG0580029157.01.T01:intron 45.0%
CTGGAGCTTCAACAAACTTG+CGG - Chr5:93020244-93020263 MsG0580029157.01.T01:intron 45.0%
CTTCTTCCCAAACCTTATCC+GGG - Chr5:93019666-93019685 MsG0580029157.01.T01:three_prime_UTR 45.0%
GAAATGTCTTGGGTCTACTG+GGG - Chr5:93022511-93022530 MsG0580029157.01.T01:intron 45.0%
GAACAGAGGGAGTCTTTACT+TGG + Chr5:93023727-93023746 None:intergenic 45.0%
GATCCAAGTCCCATTTCACA+AGG + Chr5:93021994-93022013 None:intergenic 45.0%
GCGGAACAGCGTATGAATTA+CGG + Chr5:93019811-93019830 None:intergenic 45.0%
GGAGAATATTTGGCTGGTAC+TGG + Chr5:93023706-93023725 None:intergenic 45.0%
GTTGACACAGGATTTGCAGA+TGG - Chr5:93021035-93021054 MsG0580029157.01.T01:intron 45.0%
TGAGGTCTCTTTGTAGTGAG+TGG - Chr5:93023453-93023472 MsG0580029157.01.T01:intron 45.0%
TTGTTCCGTGACATAGCTAG+CGG - Chr5:93020050-93020069 MsG0580029157.01.T01:three_prime_UTR 45.0%
TTTGTTGAAGCTCCAGAGTG+TGG + Chr5:93020240-93020259 None:intergenic 45.0%
! ATGGATTAATGCACTGCCAC+CGG + Chr5:93020973-93020992 None:intergenic 45.0%
! GCTTGTGAATGATAGCTTGC+AGG - Chr5:93023007-93023026 MsG0580029157.01.T01:intron 45.0%
! TGATCAACGGACTCATTGTG+CGG + Chr5:93019830-93019849 None:intergenic 45.0%
! TGCAGGTATTCAACTGGTCT+TGG - Chr5:93020942-93020961 MsG0580029157.01.T01:intron 45.0%
! TTTTGGCAAAAGCCACACTC+TGG - Chr5:93020225-93020244 MsG0580029157.01.T01:intron 45.0%
!! CGGTGTTCAATTTTTCAGCC+AGG + Chr5:93023280-93023299 None:intergenic 45.0%
!! GTACAATTTGGTGCATTGGG+TGG - Chr5:93022246-93022265 MsG0580029157.01.T01:CDS 45.0%
!! TCAACTGGTCTTGGAGTAAC+CGG - Chr5:93020951-93020970 MsG0580029157.01.T01:intron 45.0%
!!! CCTTGGGATTCACTTTTTGG+TGG + Chr5:93020814-93020833 None:intergenic 45.0%
!!! GGTTTTAAGAGTAAGACGCG+CGG - Chr5:93022349-93022368 MsG0580029157.01.T01:CDS 45.0%
!! AATAAATTTAATAAATTGTA+GGG - Chr5:93023077-93023096 MsG0580029157.01.T01:intron 5.0%
!!! ACTTTTAAATAAAAAAAATT+TGG - Chr5:93020523-93020542 MsG0580029157.01.T01:CDS 5.0%
AACGAGAGGATGAACCTTCC+CGG + Chr5:93019687-93019706 None:intergenic 50.0%
AGGATGAACCTTCCCGGATA+AGG + Chr5:93019681-93019700 None:intergenic 50.0%
ATGGTTGAAGCTGAGCGCAT+TGG - Chr5:93021152-93021171 MsG0580029157.01.T01:intron 50.0%
CACAGGATTTGCAGATGGAG+AGG - Chr5:93021040-93021059 MsG0580029157.01.T01:intron 50.0%
GAACCTTCCCGGATAAGGTT+TGG + Chr5:93019676-93019695 None:intergenic 50.0%
GCACAATGAGTCCGTTGATC+AGG - Chr5:93019829-93019848 MsG0580029157.01.T01:three_prime_UTR 50.0%
TTAGGTGTTTGGCCTCCAGA+TGG - Chr5:93022328-93022347 MsG0580029157.01.T01:CDS 50.0%
TTCCCAAACCTTATCCGGGA+AGG - Chr5:93019670-93019689 MsG0580029157.01.T01:three_prime_UTR 50.0%
! AGACGCGCGGTCTCTTTTAA+AGG - Chr5:93022362-93022381 MsG0580029157.01.T01:CDS 50.0%
! GACGCGCGGTCTCTTTTAAA+GGG - Chr5:93022363-93022382 MsG0580029157.01.T01:CDS 50.0%
!! ACGCGCGGTCTCTTTTAAAG+GGG - Chr5:93022364-93022383 MsG0580029157.01.T01:CDS 50.0%
!! GGCCTGACAAGAGTGTTTCA+TGG - Chr5:93023197-93023216 MsG0580029157.01.T01:CDS 50.0%
!!! AAATGCCGAGGAGGGCATTT+TGG + Chr5:93022982-93023001 None:intergenic 50.0%
!!! AATGCCGAGGAGGGCATTTT+GGG + Chr5:93022981-93023000 None:intergenic 50.0%
ACGGATATCGACGGTTCCGT+CGG + Chr5:93019792-93019811 None:intergenic 55.0%
CCTTCCCAAAATGCCCTCCT+CGG - Chr5:93022974-93022993 MsG0580029157.01.T01:intron 55.0%
! TGATACTGGACTGGAGGCAG+TGG - Chr5:93021594-93021613 MsG0580029157.01.T01:intron 55.0%
!! ACTGGTCTTGGAGTAACCGG+TGG - Chr5:93020954-93020973 MsG0580029157.01.T01:intron 55.0%
!!! CGAGGAGGGCATTTTGGGAA+GGG + Chr5:93022976-93022995 None:intergenic 55.0%
CACAAGGACACAGCTCAGCC+TGG - Chr5:93023259-93023278 MsG0580029157.01.T01:intron 60.0%
CGCGTCATCGGAACACTCCT+CGG - Chr5:93019755-93019774 MsG0580029157.01.T01:three_prime_UTR 60.0%
CGGATATCGACGGTTCCGTC+GGG + Chr5:93019791-93019810 None:intergenic 60.0%
GGATATCGACGGTTCCGTCG+GGG + Chr5:93019790-93019809 None:intergenic 60.0%
TGTCCGTCGTCCTGACCAAG+CGG - Chr5:93019730-93019749 MsG0580029157.01.T01:three_prime_UTR 60.0%
!!! CCGAGGAGGGCATTTTGGGA+AGG + Chr5:93022977-93022996 None:intergenic 60.0%
ATGACGCGCTCCGCTTGGTC+AGG + Chr5:93019743-93019762 None:intergenic 65.0%
GACCAAGCGGAGCGCGTCAT+CGG - Chr5:93019743-93019762 MsG0580029157.01.T01:three_prime_UTR 65.0%
GACGGTTCCGTCGGGGAGAA+CGG + Chr5:93019783-93019802 None:intergenic 65.0%
GCTCCGCTTGGTCAGGACGA+CGG + Chr5:93019736-93019755 None:intergenic 65.0%
TTCCGATGACGCGCTCCGCT+TGG + Chr5:93019748-93019767 None:intergenic 65.0%
CTCGGCTCCGTTCTCCCCGA+CGG - Chr5:93019773-93019792 MsG0580029157.01.T01:three_prime_UTR 70.0%
CGTCGGGGAGAACGGAGCCG+AGG + Chr5:93019775-93019794 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 93019632 93023771 93019632 ID=MsG0580029157.01;Name=MsG0580029157.01
Chr5 mRNA 93019632 93023771 93019632 ID=MsG0580029157.01.T01;Parent=MsG0580029157.01;Name=MsG0580029157.01.T01;_AED=0.49;_eAED=0.52;_QI=0|0.66|0.71|1|0.83|0.71|7|433|309
Chr5 exon 93019632 93019696 93019632 ID=MsG0580029157.01.T01:exon:49608;Parent=MsG0580029157.01.T01
Chr5 exon 93019719 93020176 93019719 ID=MsG0580029157.01.T01:exon:49607;Parent=MsG0580029157.01.T01
Chr5 CDS 93023566 93023771 93023566 ID=MsG0580029157.01.T01:cds;Parent=MsG0580029157.01.T01
Chr5 CDS 93023138 93023225 93023138 ID=MsG0580029157.01.T01:cds;Parent=MsG0580029157.01.T01
Chr5 CDS 93022554 93022633 93022554 ID=MsG0580029157.01.T01:cds;Parent=MsG0580029157.01.T01
Chr5 CDS 93022227 93022456 93022227 ID=MsG0580029157.01.T01:cds;Parent=MsG0580029157.01.T01
Chr5 CDS 93020375 93020610 93020375 ID=MsG0580029157.01.T01:cds;Parent=MsG0580029157.01.T01
Chr5 CDS 93020087 93020176 93020087 ID=MsG0580029157.01.T01:cds;Parent=MsG0580029157.01.T01
Chr5 three_prime_UTR 93019719 93020086 93019719 ID=MsG0580029157.01.T01:three_prime_utr;Parent=MsG0580029157.01.T01
Chr5 three_prime_UTR 93019632 93019696 93019632 ID=MsG0580029157.01.T01:three_prime_utr;Parent=MsG0580029157.01.T01
Chr5 mRNA 93019659 93023771 93019659 ID=MsG0580029157.01.T02;Parent=MsG0580029157.01;Name=MsG0580029157.01.T02;_AED=0.49;_eAED=0.52;_QI=0|0.6|0.66|1|1|1|6|428|309
Chr5 exon 93019659 93020176 93019659 ID=MsG0580029157.01.T02:exon:49609;Parent=MsG0580029157.01.T02
Chr5 CDS 93023566 93023771 93023566 ID=MsG0580029157.01.T02:cds;Parent=MsG0580029157.01.T02
Chr5 CDS 93023138 93023225 93023138 ID=MsG0580029157.01.T02:cds;Parent=MsG0580029157.01.T02
Chr5 CDS 93022554 93022633 93022554 ID=MsG0580029157.01.T02:cds;Parent=MsG0580029157.01.T02
Chr5 CDS 93022227 93022456 93022227 ID=MsG0580029157.01.T02:cds;Parent=MsG0580029157.01.T02
Chr5 CDS 93020375 93020610 93020375 ID=MsG0580029157.01.T02:cds;Parent=MsG0580029157.01.T02
Chr5 CDS 93020087 93020176 93020087 ID=MsG0580029157.01.T02:cds;Parent=MsG0580029157.01.T02
Chr5 three_prime_UTR 93019659 93020086 93019659 ID=MsG0580029157.01.T02:three_prime_utr;Parent=MsG0580029157.01.T02
Gene Sequence

>MsG0580029157.01.T02

ATGGCGGCGAGTAAGCGCACTGTGTTGCAATTCTCTTCTTCCCAAACCTTATCCGGGAAGGTTCATCCTCTCGTTATCTTCAACATCTGTGATTGCTATGTCCGTCGTCCTGACCAAGCGGAGCGCGTCATCGGAACACTCCTCGGCTCCGTTCTCCCCGACGGAACCGTCGATATCCGTAATTCATACGCTGTTCCGCACAATGAATTAATGCGATTTTGGCAAAAGCCACACTATGGAGTTCAAAATGCTATTTTGGCAAAAGCCACACTCTGGAGCTTCAACAAACTTGCGGTTGCTTTGGATATTGAGTACCACCATAATATGTTACTATCCCACCAAAAAGTGAATCCCAAGGAAGCTATAGTTGGATGGTATTCAACTGGTCTTGGAGTAACCGGTGGCAGTGCATTAATCCATGAATTTTATTCTCGAGAAGTATCCAACCCAATACATTTGACTGTTGACACAGGATTTGCAGATGGAGAGGGCACCATAAAAGCATATGTTTCAAACAATTTATCACTTGGAGATCAGCAAATTGCTGCACAGTTTCAAGAAATTCCTTTGGATCTTCGTATGGTTGAAGCTGAGCGCATTGGATTTGATAATCTGAAGTCAACTTCTGTTGACAAAATCCCATCTGATTTAGAAGGGATGGAAGCATCAATGACACATCTATTGGCCTTGGTTGATGACACCTACAAATACGTCAATGATGTTGTGGAAGGGCGTGTTGACCCAGACAACAAAATTGGGAAATGTATATCAGATGTTGTAGGTTCCCTTCCCAAAATGCCCTCCTCGGCATTTGATAAGCTTGTGAATGATAGCTTGCAGGATCATTTGCTCATGGTATATTTATCTAGCATCACAAGGACACAGCTCAGCCTGGCTGAAAAATTGAACACCGCTGCTCAGATTCTGTGA

>MsG0580029157.01.T01

ATGGCGGCGAGTAAGCGCACTGTGTTGCAATTCTCTTCTTCCCAAACCTTATCCGGGAAGGTTCATCCTCTCGTTATCTTCAACATCTGTGATTGCTATGTCCGTCGTCCTGACCAAGCGGAGCGCGTCATCGGAACACTCCTCGGCTCCGTTCTCCCCGACGGAACCGTCGATATCCGTAATTCATACGCTGTTCCGCACAATGAATTAATGCGATTTTGGCAAAAGCCACACTATGGAGTTCAAAATGCTATTTTGGCAAAAGCCACACTCTGGAGCTTCAACAAACTTGCGGTTGCTTTGGATATTGAGTACCACCATAATATGTTACTATCCCACCAAAAAGTGAATCCCAAGGAAGCTATAGTTGGATGGTATTCAACTGGTCTTGGAGTAACCGGTGGCAGTGCATTAATCCATGAATTTTATTCTCGAGAAGTATCCAACCCAATACATTTGACTGTTGACACAGGATTTGCAGATGGAGAGGGCACCATAAAAGCATATGTTTCAAACAATTTATCACTTGGAGATCAGCAAATTGCTGCACAGTTTCAAGAAATTCCTTTGGATCTTCGTATGGTTGAAGCTGAGCGCATTGGATTTGATAATCTGAAGTCAACTTCTGTTGACAAAATCCCATCTGATTTAGAAGGGATGGAAGCATCAATGACACATCTATTGGCCTTGGTTGATGACACCTACAAATACGTCAATGATGTTGTGGAAGGGCGTGTTGACCCAGACAACAAAATTGGGAAATGTATATCAGATGTTGTAGGTTCCCTTCCCAAAATGCCCTCCTCGGCATTTGATAAGCTTGTGAATGATAGCTTGCAGGATCATTTGCTCATGGTATATTTATCTAGCATCACAAGGACACAGCTCAGCCTGGCTGAAAAATTGAACACCGCTGCTCAGATTCTGTGA
Protein sequence

>MsG0580029157.01.T02

MAASKRTVLQFSSSQTLSGKVHPLVIFNICDCYVRRPDQAERVIGTLLGSVLPDGTVDIRNSYAVPHNELMRFWQKPHYGVQNAILAKATLWSFNKLAVALDIEYHHNMLLSHQKVNPKEAIVGWYSTGLGVTGGSALIHEFYSREVSNPIHLTVDTGFADGEGTIKAYVSNNLSLGDQQIAAQFQEIPLDLRMVEAERIGFDNLKSTSVDKIPSDLEGMEASMTHLLALVDDTYKYVNDVVEGRVDPDNKIGKCISDVVGSLPKMPSSAFDKLVNDSLQDHLLMVYLSSITRTQLSLAEKLNTAAQIL*

>MsG0580029157.01.T01

MAASKRTVLQFSSSQTLSGKVHPLVIFNICDCYVRRPDQAERVIGTLLGSVLPDGTVDIRNSYAVPHNELMRFWQKPHYGVQNAILAKATLWSFNKLAVALDIEYHHNMLLSHQKVNPKEAIVGWYSTGLGVTGGSALIHEFYSREVSNPIHLTVDTGFADGEGTIKAYVSNNLSLGDQQIAAQFQEIPLDLRMVEAERIGFDNLKSTSVDKIPSDLEGMEASMTHLLALVDDTYKYVNDVVEGRVDPDNKIGKCISDVVGSLPKMPSSAFDKLVNDSLQDHLLMVYLSSITRTQLSLAEKLNTAAQIL*