AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018486.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018486.01.T01 MTR_4g013135 97.531 162 4 0 1 162 152 313 1.54e-113 325
MsG0480018486.01.T01 MTR_4g013090 92.593 162 12 0 1 162 152 313 4.02e-108 311
MsG0480018486.01.T01 MTR_4g013100 92.593 162 12 0 1 162 153 314 2.85e-107 309
MsG0480018486.01.T01 MTR_4g012960 90.123 162 16 0 1 162 149 310 1.81e-105 304
MsG0480018486.01.T01 MTR_2g101840 68.098 163 51 1 1 162 152 314 1.21e-74 226
MsG0480018486.01.T01 MTR_8g033390 49.383 162 82 0 1 162 162 323 2.45e-56 179
MsG0480018486.01.T01 MTR_8g033410 49.383 162 82 0 1 162 165 326 2.52e-53 172
MsG0480018486.01.T01 MTR_8g033400 48.148 162 84 0 1 162 172 333 4.72e-52 169
MsG0480018486.01.T01 MTR_7g075830 51.899 158 75 1 4 161 158 314 3.86e-50 163
MsG0480018486.01.T01 MTR_1g112510 31.638 177 101 4 2 158 174 350 4.56e-19 82.8
MsG0480018486.01.T01 MTR_6g007170 29.878 164 105 5 2 157 208 369 5.47e-14 68.6
MsG0480018486.01.T01 MTR_4g005880 29.545 176 106 4 2 162 236 408 1.48e-13 67.8
MsG0480018486.01.T01 MTR_3g005970 29.730 111 77 1 35 144 252 362 5.52e-13 65.9
MsG0480018486.01.T01 MTR_1g107480 29.240 171 111 5 1 162 202 371 8.24e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018486.01.T01 AT1G79870 76.543 162 38 0 1 162 152 313 2.21e-88 261
MsG0480018486.01.T01 AT1G79870 76.543 162 38 0 1 162 133 294 2.23e-88 260
MsG0480018486.01.T01 AT1G12550 55.280 161 72 0 1 161 160 320 3.25e-60 189
MsG0480018486.01.T01 AT2G45630 47.853 163 84 1 1 162 176 338 5.26e-50 164
MsG0480018486.01.T01 AT1G68010 29.379 177 105 3 2 158 174 350 8.35e-18 79.3
MsG0480018486.01.T01 AT5G14780 30.909 165 106 4 2 159 180 343 3.56e-17 77.4
MsG0480018486.01.T01 AT1G68010 30.247 162 93 3 2 143 174 335 3.79e-17 77.4
MsG0480018486.01.T01 AT5G14780 30.909 165 106 4 2 159 192 355 3.84e-17 77.4
MsG0480018486.01.T01 AT5G14780 30.909 165 106 4 2 159 206 369 4.45e-17 77.4
MsG0480018486.01.T01 AT1G68010 29.213 178 105 4 2 158 174 351 3.89e-16 74.7
MsG0480018486.01.T01 AT1G17745 33.908 174 101 6 2 162 257 429 6.03e-16 74.7
MsG0480018486.01.T01 AT1G17745 32.370 173 105 4 2 162 230 402 6.34e-16 74.3
MsG0480018486.01.T01 AT5G28310 32.515 163 58 5 1 162 122 233 3.56e-15 70.5
MsG0480018486.01.T01 AT4G34200 29.825 171 110 5 1 162 208 377 2.85e-14 69.7
MsG0480018486.01.T01 AT1G72190 28.758 153 99 2 2 144 201 353 1.12e-13 67.8

Find 55 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGATAGTACTTGTATTT+TGG 0.175534 4:-5617282 None:intergenic
CATGTTCCCGAAGAACTATT+TGG 0.241347 4:+5617528 MsG0480018486.01.T01:CDS
TGCATTACTTGAAGGTCGTT+TGG 0.291318 4:+5617470 MsG0480018486.01.T01:CDS
TACCTCGGCCAATGTTAATT+AGG 0.297605 4:-5617419 None:intergenic
GCCATGGCCGACCTTCTTCT+TGG 0.315388 4:+5617609 MsG0480018486.01.T01:CDS
TTCTAGCCCAAATAGTTCTT+CGG 0.356251 4:-5617535 None:intergenic
TCTAGCCCAAATAGTTCTTC+GGG 0.361285 4:-5617534 None:intergenic
CGACCTTCTTCTTGGAAACT+TGG 0.365789 4:+5617617 MsG0480018486.01.T01:CDS
GCATTACTTGAAGGTCGTTT+GGG 0.382800 4:+5617471 MsG0480018486.01.T01:CDS
AACTTGGAGGCTCATTTCCT+TGG 0.395330 4:+5617633 MsG0480018486.01.T01:CDS
ATTAAAACCTTCAGCTCTCT+TGG 0.401991 4:-5617220 None:intergenic
CCTTCTTCTTGGAAACTTGG+AGG 0.417287 4:+5617620 MsG0480018486.01.T01:CDS
ATGTTCCCGAAGAACTATTT+GGG 0.421570 4:+5617529 MsG0480018486.01.T01:CDS
CTTTGGAGTTCTAGAATAGT+AGG 0.425937 4:-5617253 None:intergenic
AAACTCATCACATCATCAAC+CGG 0.440972 4:+5617364 MsG0480018486.01.T01:CDS
AGCATCCAACTGCGACATAC+TGG 0.457202 4:+5617317 MsG0480018486.01.T01:CDS
CTGGTCTCTGCATTACTTGA+AGG 0.461779 4:+5617462 MsG0480018486.01.T01:CDS
GAAGGTCGTTTGGGTGGTGC+TGG 0.462451 4:+5617480 MsG0480018486.01.T01:CDS
AAAGCCACTGTTAACTCCCT+TGG 0.464385 4:+5617656 MsG0480018486.01.T01:CDS
TGATGAGTTTCCTCTGTAAG+AGG 0.469038 4:-5617351 None:intergenic
ATTATTGGAATGGGAAGGAT+TGG 0.483559 4:+5617186 None:intergenic
CGGGAGGTCATTAATGCACT+TGG 0.501315 4:+5617384 MsG0480018486.01.T01:CDS
ATGTTAATTAGGACACCCTT+CGG 0.518527 4:-5617408 None:intergenic
CATTAATGCACTTGGTCCGA+AGG 0.522710 4:+5617392 MsG0480018486.01.T01:CDS
GCAGTTGCCAAGAGAGCTGA+AGG 0.536835 4:+5617213 MsG0480018486.01.T01:CDS
TTATTGCCTCATGTTGGAAG+CGG 0.540920 4:+5617567 MsG0480018486.01.T01:CDS
CCTCCAAGTTTCCAAGAAGA+AGG 0.547234 4:-5617620 None:intergenic
TCCAAGAAGAAGGTCGGCCA+TGG 0.548832 4:-5617610 None:intergenic
GATGCTAACTCAACAAGACT+TGG 0.554749 4:-5617300 None:intergenic
GGTAGAAACTCGAGCTGCCA+TGG 0.556384 4:+5617593 MsG0480018486.01.T01:CDS
GAGTTAACAGTGGCTTTCCA+AGG 0.557323 4:-5617650 None:intergenic
GCATGTTGATGAGCCAGAGC+TGG 0.562230 4:+5617443 MsG0480018486.01.T01:CDS
GTCCTAATTAACATTGGCCG+AGG 0.565769 4:+5617417 MsG0480018486.01.T01:CDS
TGTTGCCTGCCCTCTTACAG+AGG 0.566261 4:+5617341 MsG0480018486.01.T01:CDS
AGTTTCCTCTGTAAGAGGGC+AGG 0.567739 4:-5617346 None:intergenic
AGTAATGCAGAGACCAGCTC+TGG 0.569099 4:-5617456 None:intergenic
CAAGTGCATTAATGACCTCC+CGG 0.577512 4:-5617383 None:intergenic
TTGGCATTATTGGAATGGGA+AGG 0.585206 4:+5617181 None:intergenic
CTATTCTAGAACTCCAAAGC+AGG 0.594716 4:+5617257 MsG0480018486.01.T01:CDS
GCCTCATGTTGGAAGCGGCA+CGG 0.602877 4:+5617572 MsG0480018486.01.T01:CDS
ATTAATGCACTTGGTCCGAA+GGG 0.608941 4:+5617393 MsG0480018486.01.T01:CDS
AACAACCAGTATGTCGCAGT+TGG 0.611894 4:-5617322 None:intergenic
CGGCATGCAGATTAAACCAA+GGG 0.612509 4:-5617672 None:intergenic
AATAGTTCTTCGGGAACATG+AGG 0.622864 4:-5617525 None:intergenic
AAGGGTGTCCTAATTAACAT+TGG 0.623755 4:+5617411 MsG0480018486.01.T01:CDS
ACCGTGCCGCTTCCAACATG+AGG 0.623796 4:-5617573 None:intergenic
CAAGTTTCCAAGAAGAAGGT+CGG 0.631558 4:-5617616 None:intergenic
GTTGTCTTATTGCCTCATGT+TGG 0.632321 4:+5617561 MsG0480018486.01.T01:CDS
GATGAGTTTCCTCTGTAAGA+GGG 0.642427 4:-5617350 None:intergenic
TTACTTGAAGGTCGTTTGGG+TGG 0.644249 4:+5617474 MsG0480018486.01.T01:CDS
AACTCATCACATCATCAACC+GGG 0.655089 4:+5617365 MsG0480018486.01.T01:CDS
GCGGCATGCAGATTAAACCA+AGG 0.659732 4:-5617673 None:intergenic
TAAACCAAGGGAGTTAACAG+TGG 0.668491 4:-5617660 None:intergenic
GCTCATCAACATGCTTACCT+CGG 0.690772 4:-5617434 None:intergenic
TCATCACATCATCAACCGGG+AGG 0.750770 4:+5617368 MsG0480018486.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTGGATAGTACTTGTATTT+TGG - Chr4:5617285-5617304 None:intergenic 30.0%
AAACTCATCACATCATCAAC+CGG + Chr4:5617364-5617383 MsG0480018486.01.T01:CDS 35.0%
ATGTTAATTAGGACACCCTT+CGG - Chr4:5617411-5617430 None:intergenic 35.0%
ATGTTCCCGAAGAACTATTT+GGG + Chr4:5617529-5617548 MsG0480018486.01.T01:CDS 35.0%
ATTAAAACCTTCAGCTCTCT+TGG - Chr4:5617223-5617242 None:intergenic 35.0%
TTCTAGCCCAAATAGTTCTT+CGG - Chr4:5617538-5617557 None:intergenic 35.0%
!! AAGGGTGTCCTAATTAACAT+TGG + Chr4:5617411-5617430 MsG0480018486.01.T01:CDS 35.0%
!! CTTTGGAGTTCTAGAATAGT+AGG - Chr4:5617256-5617275 None:intergenic 35.0%
!!! TTGTATTTTGGTTCCTGCTT+TGG - Chr4:5617273-5617292 None:intergenic 35.0%
AACTCATCACATCATCAACC+GGG + Chr4:5617365-5617384 MsG0480018486.01.T01:CDS 40.0%
AATAGTTCTTCGGGAACATG+AGG - Chr4:5617528-5617547 None:intergenic 40.0%
ATTAATGCACTTGGTCCGAA+GGG + Chr4:5617393-5617412 MsG0480018486.01.T01:CDS 40.0%
CAAGTTTCCAAGAAGAAGGT+CGG - Chr4:5617619-5617638 None:intergenic 40.0%
CATGTTCCCGAAGAACTATT+TGG + Chr4:5617528-5617547 MsG0480018486.01.T01:CDS 40.0%
GATGAGTTTCCTCTGTAAGA+GGG - Chr4:5617353-5617372 None:intergenic 40.0%
GATGCTAACTCAACAAGACT+TGG - Chr4:5617303-5617322 None:intergenic 40.0%
GCATTACTTGAAGGTCGTTT+GGG + Chr4:5617471-5617490 MsG0480018486.01.T01:CDS 40.0%
TAAACCAAGGGAGTTAACAG+TGG - Chr4:5617663-5617682 None:intergenic 40.0%
TACCTCGGCCAATGTTAATT+AGG - Chr4:5617422-5617441 None:intergenic 40.0%
TCTAGCCCAAATAGTTCTTC+GGG - Chr4:5617537-5617556 None:intergenic 40.0%
TGATGAGTTTCCTCTGTAAG+AGG - Chr4:5617354-5617373 None:intergenic 40.0%
TGCATTACTTGAAGGTCGTT+TGG + Chr4:5617470-5617489 MsG0480018486.01.T01:CDS 40.0%
TTATTGCCTCATGTTGGAAG+CGG + Chr4:5617567-5617586 MsG0480018486.01.T01:CDS 40.0%
! GTTGTCTTATTGCCTCATGT+TGG + Chr4:5617561-5617580 MsG0480018486.01.T01:CDS 40.0%
!! CTATTCTAGAACTCCAAAGC+AGG + Chr4:5617257-5617276 MsG0480018486.01.T01:CDS 40.0%
AAAGCCACTGTTAACTCCCT+TGG + Chr4:5617656-5617675 MsG0480018486.01.T01:CDS 45.0%
AACAACCAGTATGTCGCAGT+TGG - Chr4:5617325-5617344 None:intergenic 45.0%
AACTTGGAGGCTCATTTCCT+TGG + Chr4:5617633-5617652 MsG0480018486.01.T01:CDS 45.0%
CAAGTGCATTAATGACCTCC+CGG - Chr4:5617386-5617405 None:intergenic 45.0%
CATTAATGCACTTGGTCCGA+AGG + Chr4:5617392-5617411 MsG0480018486.01.T01:CDS 45.0%
CCTCCAAGTTTCCAAGAAGA+AGG - Chr4:5617623-5617642 None:intergenic 45.0%
CCTTCTTCTTGGAAACTTGG+AGG + Chr4:5617620-5617639 MsG0480018486.01.T01:CDS 45.0%
CGACCTTCTTCTTGGAAACT+TGG + Chr4:5617617-5617636 MsG0480018486.01.T01:CDS 45.0%
CTGGTCTCTGCATTACTTGA+AGG + Chr4:5617462-5617481 MsG0480018486.01.T01:CDS 45.0%
GAGTTAACAGTGGCTTTCCA+AGG - Chr4:5617653-5617672 None:intergenic 45.0%
GCTCATCAACATGCTTACCT+CGG - Chr4:5617437-5617456 None:intergenic 45.0%
GTCCTAATTAACATTGGCCG+AGG + Chr4:5617417-5617436 MsG0480018486.01.T01:CDS 45.0%
TTACTTGAAGGTCGTTTGGG+TGG + Chr4:5617474-5617493 MsG0480018486.01.T01:CDS 45.0%
AGCATCCAACTGCGACATAC+TGG + Chr4:5617317-5617336 MsG0480018486.01.T01:CDS 50.0%
AGTAATGCAGAGACCAGCTC+TGG - Chr4:5617459-5617478 None:intergenic 50.0%
AGTTTCCTCTGTAAGAGGGC+AGG - Chr4:5617349-5617368 None:intergenic 50.0%
CGGGAGGTCATTAATGCACT+TGG + Chr4:5617384-5617403 MsG0480018486.01.T01:CDS 50.0%
TCATCACATCATCAACCGGG+AGG + Chr4:5617368-5617387 MsG0480018486.01.T01:CDS 50.0%
GCAGTTGCCAAGAGAGCTGA+AGG + Chr4:5617213-5617232 MsG0480018486.01.T01:CDS 55.0%
GGTAGAAACTCGAGCTGCCA+TGG + Chr4:5617593-5617612 MsG0480018486.01.T01:CDS 55.0%
TCCAAGAAGAAGGTCGGCCA+TGG - Chr4:5617613-5617632 None:intergenic 55.0%
TGTTGCCTGCCCTCTTACAG+AGG + Chr4:5617341-5617360 MsG0480018486.01.T01:CDS 55.0%
! GCATGTTGATGAGCCAGAGC+TGG + Chr4:5617443-5617462 MsG0480018486.01.T01:CDS 55.0%
GAAGGTCGTTTGGGTGGTGC+TGG + Chr4:5617480-5617499 MsG0480018486.01.T01:CDS 60.0%
GCCATGGCCGACCTTCTTCT+TGG + Chr4:5617609-5617628 MsG0480018486.01.T01:CDS 60.0%
GCCTCATGTTGGAAGCGGCA+CGG + Chr4:5617572-5617591 MsG0480018486.01.T01:CDS 60.0%
!! ACCGTGCCGCTTCCAACATG+AGG - Chr4:5617576-5617595 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 5617195 5617683 5617195 ID=MsG0480018486.01;Name=MsG0480018486.01
Chr4 mRNA 5617195 5617683 5617195 ID=MsG0480018486.01.T01;Parent=MsG0480018486.01;Name=MsG0480018486.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|162
Chr4 exon 5617195 5617683 5617195 ID=MsG0480018486.01.T01:exon:1976;Parent=MsG0480018486.01.T01
Chr4 CDS 5617195 5617683 5617195 ID=MsG0480018486.01.T01:cds;Parent=MsG0480018486.01.T01
Gene Sequence

>MsG0480018486.01.T01

ATGGGAAGGATTGGCACAGCAGTTGCCAAGAGAGCTGAAGGTTTTAATTGTTCAATATCCTACTATTCTAGAACTCCAAAGCAGGAACCAAAATACAAGTACTATCCAAGTCTTGTTGAGTTAGCATCCAACTGCGACATACTGGTTGTTGCCTGCCCTCTTACAGAGGAAACTCATCACATCATCAACCGGGAGGTCATTAATGCACTTGGTCCGAAGGGTGTCCTAATTAACATTGGCCGAGGTAAGCATGTTGATGAGCCAGAGCTGGTCTCTGCATTACTTGAAGGTCGTTTGGGTGGTGCTGGACTTGATGTGTATGAAAACGAGCCTCATGTTCCCGAAGAACTATTTGGGCTAGAAAATGTTGTCTTATTGCCTCATGTTGGAAGCGGCACGGTAGAAACTCGAGCTGCCATGGCCGACCTTCTTCTTGGAAACTTGGAGGCTCATTTCCTTGGAAAGCCACTGTTAACTCCCTTGGTTTAA

Protein sequence

>MsG0480018486.01.T01

MGRIGTAVAKRAEGFNCSISYYSRTPKQEPKYKYYPSLVELASNCDILVVACPLTEETHHIINREVINALGPKGVLINIGRGKHVDEPELVSALLEGRLGGAGLDVYENEPHVPEELFGLENVVLLPHVGSGTVETRAAMADLLLGNLEAHFLGKPLLTPLV*