AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032762.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032762.01.T01 MTR_6g047800 95.793 309 12 1 18 325 1 309 0.0 609
MsG0680032762.01.T01 MTR_6g047800 95.667 300 12 1 27 325 1 300 0.0 569
MsG0680032762.01.T01 MTR_2g014590 77.489 231 52 0 18 248 2 232 7.20e-136 385
MsG0680032762.01.T01 MTR_2g014570 66.102 59 17 2 266 324 7 62 8.13e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032762.01.T01 AT5G14600 73.333 315 77 2 18 325 1 315 1.22e-173 484

Find 78 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTCATTGTTATGTATT+TGG 0.122846 6:+49009765 MsG0680032762.01.T01:CDS
ACAATTATGTGGAAGGTTTC+TGG 0.176634 6:+49011951 MsG0680032762.01.T01:three_prime_UTR
GATGAAGAGGTTTGATTTAA+TGG 0.223569 6:+49012073 MsG0680032762.01.T01:three_prime_UTR
TCCTGAAGAGTTTGCTGGCT+TGG 0.242918 6:+49011361 MsG0680032762.01.T01:CDS
TAGCTCCTACTCCTGAATTA+TGG 0.261887 6:+49009695 MsG0680032762.01.T01:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 6:+49009516 MsG0680032762.01.T01:CDS
TCCCAATTCTTCTTCTGTTA+TGG 0.281081 6:+49011780 MsG0680032762.01.T01:CDS
GGTTGCGTCGTTCTTGAATC+TGG 0.281471 6:+49009799 MsG0680032762.01.T01:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 6:+49011666 MsG0680032762.01.T01:CDS
GTCTAAAGTATTCAACAATA+AGG 0.308450 6:+49009654 MsG0680032762.01.T01:CDS
TTGTTTATGTGAAAGATATA+AGG 0.311677 6:+49011617 MsG0680032762.01.T01:intron
CCAAACATGAATTTCCTATA+TGG 0.311905 6:-49011986 None:intergenic
GATTGGATAGGAAAGTCATT+TGG 0.331486 6:+49009631 MsG0680032762.01.T01:CDS
AAAACTAAAGTCCATAATTC+AGG 0.343342 6:-49009706 None:intergenic
CTTGAATCTGGCACTGGAAG+TGG 0.346516 6:+49009811 MsG0680032762.01.T01:CDS
GCCAAGCCAGCAAACTCTTC+AGG 0.354828 6:-49011362 None:intergenic
AGCCATAACAGAAGAAGAAT+TGG 0.358884 6:-49011782 None:intergenic
ATGAAGAGGTTTGATTTAAT+GGG 0.372610 6:+49012074 MsG0680032762.01.T01:three_prime_UTR
GGTATTTGTAATCTCCATAT+AGG 0.372906 6:+49011972 MsG0680032762.01.T01:three_prime_UTR
ATGTATTTGGAGATTGTTCC+TGG 0.385811 6:+49009778 MsG0680032762.01.T01:CDS
CCATATAGGAAATTCATGTT+TGG 0.417762 6:+49011986 MsG0680032762.01.T01:three_prime_UTR
GATCCCTCAGATACTGTTAC+TGG 0.419969 6:-49009571 None:intergenic
ATTGGATAGGAAAGTCATTT+GGG 0.422230 6:+49009632 MsG0680032762.01.T01:CDS
GACGGTAATGATGGCTCTAA+TGG 0.423313 6:+49011703 MsG0680032762.01.T01:CDS
AGGGATGACTTTGAGAGGAT+TGG 0.424669 6:+49011287 MsG0680032762.01.T01:intron
TAATTCTGTATTACAGTCAC+TGG 0.463847 6:+49012051 MsG0680032762.01.T01:three_prime_UTR
TCTAAAGTATTCAACAATAA+GGG 0.468151 6:+49009655 MsG0680032762.01.T01:CDS
CTTAATTCACAACAATTATG+TGG 0.469309 6:+49011940 MsG0680032762.01.T01:three_prime_UTR
TCGGGAAGTACCGGTGAAGT+TGG 0.488752 6:-49011522 None:intergenic
TTGAATCTGGCACTGGAAGT+GGG 0.495210 6:+49009812 MsG0680032762.01.T01:CDS
AAGAGGTTTGATTTAATGGG+TGG 0.498099 6:+49012077 MsG0680032762.01.T01:three_prime_UTR
GCCATAACAGAAGAAGAATT+GGG 0.501842 6:-49011781 None:intergenic
CTTTAACTACTTCACTTGCA+AGG 0.505822 6:+49009836 MsG0680032762.01.T01:CDS
ATTTGGCAATTGTTTATGAA+AGG 0.507354 6:+49009533 MsG0680032762.01.T01:CDS
TTTAAACATTCAGATTGGAT+AGG 0.513874 6:+49009619 MsG0680032762.01.T01:CDS
ATTCACAACAATTATGTGGA+AGG 0.514332 6:+49011944 MsG0680032762.01.T01:three_prime_UTR
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 6:+49009853 MsG0680032762.01.T01:CDS
TACTTGTTTATTGAAAATGT+TGG 0.523271 6:+49009459 MsG0680032762.01.T01:CDS
TCAGAGATTGACGGTAATGA+TGG 0.525850 6:+49011694 MsG0680032762.01.T01:CDS
CTTGTAGGGATGACTTTGAG+AGG 0.533942 6:+49011282 MsG0680032762.01.T01:intron
GTCGTTCTTGAATCTGGCAC+TGG 0.536486 6:+49009805 MsG0680032762.01.T01:CDS
CGTTGTACTTGTTCAATACA+CGG 0.539892 6:-49011479 None:intergenic
AAAGTCCATAATTCAGGAGT+AGG 0.545264 6:-49009700 None:intergenic
GCGGGAGTGAGGGACATTCA+AGG 0.551907 6:+49011329 MsG0680032762.01.T01:CDS
GAACGTTGCCTCCTCTTGCA+AGG 0.567462 6:-49011733 None:intergenic
ATAAGCAACTTAGTCACTGC+GGG 0.567539 6:+49011311 MsG0680032762.01.T01:CDS
CTTAGTCACTGCGGGAGTGA+GGG 0.568888 6:+49011319 MsG0680032762.01.T01:CDS
ACTTAGTCACTGCGGGAGTG+AGG 0.570411 6:+49011318 MsG0680032762.01.T01:CDS
GATTCAAGAACGACGCAACC+AGG 0.571390 6:-49009796 None:intergenic
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 6:-49011407 None:intergenic
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 6:+49011722 MsG0680032762.01.T01:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 6:+49009837 MsG0680032762.01.T01:CDS
GAAGCCAGTAACAGTATCTG+AGG 0.579372 6:+49009567 MsG0680032762.01.T01:CDS
ACAATGCAATCCAACTTCAC+CGG 0.584109 6:+49011512 MsG0680032762.01.T01:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 6:+49011725 MsG0680032762.01.T01:CDS
GTTGTACTTGTTCAATACAC+GGG 0.584848 6:-49011478 None:intergenic
ATTACAGTCACTGGATGAAG+AGG 0.584950 6:+49012060 MsG0680032762.01.T01:three_prime_UTR
ATGGAAAGCTCAGAGATTGA+CGG 0.586050 6:+49011685 MsG0680032762.01.T01:CDS
AGTAACAGTATCTGAGGGAT+CGG 0.586054 6:+49009573 MsG0680032762.01.T01:CDS
TTTAATAGAACAATTAGTAA+CGG 0.596180 6:+49009508 MsG0680032762.01.T01:CDS
GTGAGGGACATTCAAGGTGA+GGG 0.600743 6:+49011335 MsG0680032762.01.T01:CDS
AAGAGGAGGCAACGTTCAGA+TGG 0.606722 6:+49011739 MsG0680032762.01.T01:CDS
TGAACTCTAGCTTCACCGCA+TGG 0.610086 6:-49011808 None:intergenic
AACGTATAAACATGTCCTGT+AGG 0.611682 6:-49009868 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 6:+49011658 MsG0680032762.01.T01:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 6:-49011654 None:intergenic
ACGTTCAGATGGAGCTAGCG+TGG 0.623569 6:+49011750 MsG0680032762.01.T01:CDS
GGACATACGAAGTTCGAGAA+GGG 0.625372 6:+49011659 MsG0680032762.01.T01:CDS
AACAAAGATCTGGATCAGCT+AGG 0.636973 6:+49009902 MsG0680032762.01.T01:CDS
AGTGAGGGACATTCAAGGTG+AGG 0.642071 6:+49011334 MsG0680032762.01.T01:CDS
AAAGTATTCAACAATAAGGG+CGG 0.646223 6:+49009658 MsG0680032762.01.T01:CDS
TATATAGAATCTGTGTCCTG+TGG 0.646737 6:-49009732 None:intergenic
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 6:-49011413 None:intergenic
AAGCCAGTAACAGTATCTGA+GGG 0.661239 6:+49009568 MsG0680032762.01.T01:CDS
TCTGTTATGGCTAGACCATG+CGG 0.664012 6:+49011793 MsG0680032762.01.T01:CDS
TTGTACTTGTTCAATACACG+GGG 0.704021 6:-49011477 None:intergenic
TATAAGCAACTTAGTCACTG+CGG 0.729959 6:+49011310 MsG0680032762.01.T01:CDS
GGTGAAGCTAGAGTTCACAC+TGG 0.739750 6:+49011814 MsG0680032762.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACTTATATATTGTATTA+CGG - Chr6:49010919-49010938 None:intergenic 10.0%
!! AGTCAATTATATATAAAACA+AGG - Chr6:49010980-49010999 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG + Chr6:49009508-49009527 MsG0680032762.01.T01:CDS 15.0%
!!! TTTTTACAATGAAAAAACTT+AGG + Chr6:49010866-49010885 MsG0680032762.01.T01:intron 15.0%
!! AACATATAAAAGAGGAAAAT+CGG - Chr6:49011544-49011563 None:intergenic 20.0%
!! ACATAAACAAACATATACAA+AGG - Chr6:49011607-49011626 None:intergenic 20.0%
!! ATTTATTTAACTGTACTCAA+TGG + Chr6:49011197-49011216 MsG0680032762.01.T01:intron 20.0%
!! GTAATTGCAAGTAATATTAT+TGG + Chr6:49011130-49011149 MsG0680032762.01.T01:intron 20.0%
!! TAAAAATAAACTGTTGTATG+AGG - Chr6:49011919-49011938 None:intergenic 20.0%
!! TACTTGTTTATTGAAAATGT+TGG + Chr6:49009459-49009478 MsG0680032762.01.T01:CDS 20.0%
!! TTGTTTATGTGAAAGATATA+AGG + Chr6:49011617-49011636 MsG0680032762.01.T01:intron 20.0%
!! TTTATTTAACTGTACTCAAT+GGG + Chr6:49011198-49011217 MsG0680032762.01.T01:intron 20.0%
!!! TCTAAAGTATTCAACAATAA+GGG + Chr6:49009655-49009674 MsG0680032762.01.T01:CDS 20.0%
! AAAACTAAAGTCCATAATTC+AGG - Chr6:49009709-49009728 None:intergenic 25.0%
! ACATATAAAAGAGGAAAATC+GGG - Chr6:49011543-49011562 None:intergenic 25.0%
! AGTTGCATAACATATAAAAG+AGG - Chr6:49011552-49011571 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG + Chr6:49009959-49009978 MsG0680032762.01.T01:intron 25.0%
! ATTTGGCAATTGTTTATGAA+AGG + Chr6:49009533-49009552 MsG0680032762.01.T01:CDS 25.0%
! CTACCAAAAAACTAGAATAA+CGG - Chr6:49010088-49010107 None:intergenic 25.0%
! CTTAATTCACAACAATTATG+TGG + Chr6:49011940-49011959 MsG0680032762.01.T01:three_prime_UTR 25.0%
! TGAAATAGAAGATAGTGTTT+CGG - Chr6:49011036-49011055 None:intergenic 25.0%
! TTATTTAACTGTACTCAATG+GGG + Chr6:49011199-49011218 MsG0680032762.01.T01:intron 25.0%
! TTTAAACATTCAGATTGGAT+AGG + Chr6:49009619-49009638 MsG0680032762.01.T01:CDS 25.0%
!! ATGAAGAGGTTTGATTTAAT+GGG + Chr6:49012074-49012093 MsG0680032762.01.T01:three_prime_UTR 25.0%
!! GTCTAAAGTATTCAACAATA+AGG + Chr6:49009654-49009673 MsG0680032762.01.T01:CDS 25.0%
!! TTCATTGTTTTCTCTGTTTT+CGG + Chr6:49009428-49009447 MsG0680032762.01.T01:CDS 25.0%
!!! GAGAATGATTTTTTCAATAG+CGG + Chr6:49010645-49010664 MsG0680032762.01.T01:intron 25.0%
!!! GTGTTTTTAAACATTCAGAT+TGG + Chr6:49009614-49009633 MsG0680032762.01.T01:CDS 25.0%
!!! TCACCAAAAATACGTTTTTT+AGG - Chr6:49010205-49010224 None:intergenic 25.0%
AACAATTAGTAACGGTGATT+TGG + Chr6:49009516-49009535 MsG0680032762.01.T01:CDS 30.0%
ATTCACAACAATTATGTGGA+AGG + Chr6:49011944-49011963 MsG0680032762.01.T01:three_prime_UTR 30.0%
ATTGGATAGGAAAGTCATTT+GGG + Chr6:49009632-49009651 MsG0680032762.01.T01:CDS 30.0%
ATTGTAAAAATAAGCCACGT+TGG - Chr6:49010856-49010875 None:intergenic 30.0%
CAGCTTCATTGTTATGTATT+TGG + Chr6:49009765-49009784 MsG0680032762.01.T01:CDS 30.0%
CCAAACATGAATTTCCTATA+TGG - Chr6:49011989-49012008 None:intergenic 30.0%
CCATATAGGAAATTCATGTT+TGG + Chr6:49011986-49012005 MsG0680032762.01.T01:three_prime_UTR 30.0%
CTATCTTCTATTTCACATAG+CGG + Chr6:49011041-49011060 MsG0680032762.01.T01:intron 30.0%
GAAATAGAAGATAGTGTTTC+GGG - Chr6:49011035-49011054 None:intergenic 30.0%
GACTTATTTCAACTGTTATG+CGG + Chr6:49010996-49011015 MsG0680032762.01.T01:intron 30.0%
GCTGATATACGAACTATATT+GGG + Chr6:49010776-49010795 MsG0680032762.01.T01:intron 30.0%
GGTATTTGTAATCTCCATAT+AGG + Chr6:49011972-49011991 MsG0680032762.01.T01:three_prime_UTR 30.0%
GTGTCATCAATAGCATATTA+CGG - Chr6:49011072-49011091 None:intergenic 30.0%
TAATTCTGTATTACAGTCAC+TGG + Chr6:49012051-49012070 MsG0680032762.01.T01:three_prime_UTR 30.0%
TATTGTATTACGGTGAATAC+AGG - Chr6:49010909-49010928 None:intergenic 30.0%
TTGTAAAAATAAGCCACGTT+GGG - Chr6:49010855-49010874 None:intergenic 30.0%
TTTAACTACTTCACTTGCAA+GGG + Chr6:49009837-49009856 MsG0680032762.01.T01:CDS 30.0%
! AAAGTATTCAACAATAAGGG+CGG + Chr6:49009658-49009677 MsG0680032762.01.T01:CDS 30.0%
! AGGAGAAAAAAGAGACTTTT+AGG + Chr6:49010124-49010143 MsG0680032762.01.T01:intron 30.0%
! ATTTGATTTCCATGAGCATA+GGG + Chr6:49010531-49010550 MsG0680032762.01.T01:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG + Chr6:49011627-49011646 MsG0680032762.01.T01:intron 30.0%
! GATGAAGAGGTTTGATTTAA+TGG + Chr6:49012073-49012092 MsG0680032762.01.T01:three_prime_UTR 30.0%
! TCATTGTTTTCTCTGTTTTC+GGG + Chr6:49009429-49009448 MsG0680032762.01.T01:CDS 30.0%
! TTTCTAGTGTTGTCAACTAT+TGG + Chr6:49010000-49010019 MsG0680032762.01.T01:intron 30.0%
!! ATACTTGTTTTTCTCCTTGT+AGG + Chr6:49011267-49011286 MsG0680032762.01.T01:intron 30.0%
!! ATCTTGCAATTCATTGATTC+AGG - Chr6:49010277-49010296 None:intergenic 30.0%
!! TACTTGTTTTTCTCCTTGTA+GGG + Chr6:49011268-49011287 MsG0680032762.01.T01:intron 30.0%
!!! AAACGTATTTTTGGTGAAAC+TGG + Chr6:49010208-49010227 MsG0680032762.01.T01:intron 30.0%
!!! AACGTATTTTTGGTGAAACT+GGG + Chr6:49010209-49010228 MsG0680032762.01.T01:intron 30.0%
!!! CCAAAAATACGTTTTTTAGG+CGG - Chr6:49010202-49010221 None:intergenic 30.0%
!!! TAGTGTTGCAGTTTTTTTAG+CGG - Chr6:49010054-49010073 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG + Chr6:49010082-49010101 MsG0680032762.01.T01:intron 30.0%
AAAGTCCATAATTCAGGAGT+AGG - Chr6:49009703-49009722 None:intergenic 35.0%
AACGTATAAACATGTCCTGT+AGG - Chr6:49009871-49009890 None:intergenic 35.0%
ACAATTATGTGGAAGGTTTC+TGG + Chr6:49011951-49011970 MsG0680032762.01.T01:three_prime_UTR 35.0%
ACACATGTATCAAACAACAG+AGG - Chr6:49010605-49010624 None:intergenic 35.0%
ACCCTGCATGCAAATAAAAA+GGG - Chr6:49010956-49010975 None:intergenic 35.0%
ACTTGAGATGTTCAGTGTAA+AGG + Chr6:49011228-49011247 MsG0680032762.01.T01:intron 35.0%
AGCCATAACAGAAGAAGAAT+TGG - Chr6:49011785-49011804 None:intergenic 35.0%
ATAAACATGCCCTTCACAAA+TGG - Chr6:49010509-49010528 None:intergenic 35.0%
ATGTATTTGGAGATTGTTCC+TGG + Chr6:49009778-49009797 MsG0680032762.01.T01:CDS 35.0%
CGTTGACATTCTGAACATTA+GGG - Chr6:49011166-49011185 None:intergenic 35.0%
CGTTGTACTTGTTCAATACA+CGG - Chr6:49011482-49011501 None:intergenic 35.0%
CTTTAACTACTTCACTTGCA+AGG + Chr6:49009836-49009855 MsG0680032762.01.T01:CDS 35.0%
GATTGGATAGGAAAGTCATT+TGG + Chr6:49009631-49009650 MsG0680032762.01.T01:CDS 35.0%
GCAAAAATGTTACGACATGA+CGG + Chr6:49011437-49011456 MsG0680032762.01.T01:CDS 35.0%
GCATGCAAATAAAAAGGGAA+TGG - Chr6:49010951-49010970 None:intergenic 35.0%
GCCATAACAGAAGAAGAATT+GGG - Chr6:49011784-49011803 None:intergenic 35.0%
GGCTGATATACGAACTATAT+TGG + Chr6:49010775-49010794 MsG0680032762.01.T01:intron 35.0%
GGTTATCTTCCTTGAAATTC+CGG - Chr6:49010256-49010275 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG - Chr6:49010255-49010274 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG - Chr6:49011481-49011500 None:intergenic 35.0%
TATAAGCAACTTAGTCACTG+CGG + Chr6:49011310-49011329 MsG0680032762.01.T01:CDS 35.0%
TATATAGAATCTGTGTCCTG+TGG - Chr6:49009735-49009754 None:intergenic 35.0%
TATGTAATACTACACCAGCT+AGG + Chr6:49010564-49010583 MsG0680032762.01.T01:intron 35.0%
TCCCAATTCTTCTTCTGTTA+TGG + Chr6:49011780-49011799 MsG0680032762.01.T01:CDS 35.0%
TTGTACTTGTTCAATACACG+GGG - Chr6:49011480-49011499 None:intergenic 35.0%
! AAGAGGTTTGATTTAATGGG+TGG + Chr6:49012077-49012096 MsG0680032762.01.T01:three_prime_UTR 35.0%
! CATTTGATTTCCATGAGCAT+AGG + Chr6:49010530-49010549 MsG0680032762.01.T01:intron 35.0%
! CCGCCTAAAAAACGTATTTT+TGG + Chr6:49010199-49010218 MsG0680032762.01.T01:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG + Chr6:49009892-49009911 MsG0680032762.01.T01:CDS 35.0%
! GTAGTTGATGTAGCAACAAT+AGG + Chr6:49010104-49010123 MsG0680032762.01.T01:intron 35.0%
! TCGGTTTTACAGAATCGATT+CGG + Chr6:49009592-49009611 MsG0680032762.01.T01:CDS 35.0%
! TGCTATTGATGACACTTTCA+TGG + Chr6:49011077-49011096 MsG0680032762.01.T01:intron 35.0%
!! ATCCCTTCATAAATTTGCTG+TGG + Chr6:49010443-49010462 MsG0680032762.01.T01:intron 35.0%
!! TCCCTTTTTATTTGCATGCA+GGG + Chr6:49010952-49010971 MsG0680032762.01.T01:intron 35.0%
!! TTCCCTTTTTATTTGCATGC+AGG + Chr6:49010951-49010970 MsG0680032762.01.T01:intron 35.0%
!!! ATACGTTTTTTAGGCGGTAA+TGG - Chr6:49010196-49010215 None:intergenic 35.0%
AAAATCGCGATCAAACTCAC+GGG - Chr6:49010401-49010420 None:intergenic 40.0%
AACAAAGATCTGGATCAGCT+AGG + Chr6:49009902-49009921 MsG0680032762.01.T01:CDS 40.0%
AAGCCAGTAACAGTATCTGA+GGG + Chr6:49009568-49009587 MsG0680032762.01.T01:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG + Chr6:49011512-49011531 MsG0680032762.01.T01:CDS 40.0%
ACTATAGTGCTATCACATGG+TGG + Chr6:49010669-49010688 MsG0680032762.01.T01:intron 40.0%
AGTAACAGTATCTGAGGGAT+CGG + Chr6:49009573-49009592 MsG0680032762.01.T01:CDS 40.0%
ATAAGCAACTTAGTCACTGC+GGG + Chr6:49011311-49011330 MsG0680032762.01.T01:CDS 40.0%
ATCAAACAACAGAGGAAGTC+AGG - Chr6:49010597-49010616 None:intergenic 40.0%
ATGGAAAGCTCAGAGATTGA+CGG + Chr6:49011685-49011704 MsG0680032762.01.T01:CDS 40.0%
ATTACAGTCACTGGATGAAG+AGG + Chr6:49012060-49012079 MsG0680032762.01.T01:three_prime_UTR 40.0%
CAAAATCGCGATCAAACTCA+CGG - Chr6:49010402-49010421 None:intergenic 40.0%
CACCCTGCATGCAAATAAAA+AGG - Chr6:49010957-49010976 None:intergenic 40.0%
CGCCACAGCAAATTTATGAA+GGG - Chr6:49010448-49010467 None:intergenic 40.0%
GTATGGTCGGAATCAGAATT+TGG - Chr6:49010344-49010363 None:intergenic 40.0%
TAGCTCCTACTCCTGAATTA+TGG + Chr6:49009695-49009714 MsG0680032762.01.T01:CDS 40.0%
TCAGAGATTGACGGTAATGA+TGG + Chr6:49011694-49011713 MsG0680032762.01.T01:CDS 40.0%
TTAGGCAGTGTTGTCAATTG+TGG + Chr6:49010142-49010161 MsG0680032762.01.T01:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG - Chr6:49011431-49011450 None:intergenic 40.0%
! GCGTTGACATTCTGAACATT+AGG - Chr6:49011167-49011186 None:intergenic 40.0%
! GGCTGCTATTAAAGTTTTGC+TGG + Chr6:49010754-49010773 MsG0680032762.01.T01:intron 40.0%
! TTTTGGATCTACCTCAACCT+TGG + Chr6:49011396-49011415 MsG0680032762.01.T01:CDS 40.0%
!! CCTTCATAAATTTGCTGTGG+CGG + Chr6:49010446-49010465 MsG0680032762.01.T01:intron 40.0%
!! CTATTGGCTCTATAGCATAG+CGG + Chr6:49010016-49010035 MsG0680032762.01.T01:intron 40.0%
!! CTTCATAAATTTGCTGTGGC+GGG + Chr6:49010447-49010466 MsG0680032762.01.T01:intron 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG + Chr6:49011379-49011398 MsG0680032762.01.T01:CDS 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG + Chr6:49009716-49009735 MsG0680032762.01.T01:CDS 40.0%
AACATGCCCTTCACAAATGG+TGG - Chr6:49010506-49010525 None:intergenic 45.0%
ACCGCAGAAAATTGCAGGTA+CGG - Chr6:49010304-49010323 None:intergenic 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG + Chr6:49011722-49011741 MsG0680032762.01.T01:CDS 45.0%
CCGCCACAGCAAATTTATGA+AGG - Chr6:49010449-49010468 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG + Chr6:49011666-49011685 MsG0680032762.01.T01:CDS 45.0%
CTCTCAAAGTCATCCCTACA+AGG - Chr6:49011284-49011303 None:intergenic 45.0%
CTTGTAGGGATGACTTTGAG+AGG + Chr6:49011282-49011301 MsG0680032762.01.T01:intron 45.0%
GAAGCCAGTAACAGTATCTG+AGG + Chr6:49009567-49009586 MsG0680032762.01.T01:CDS 45.0%
GATCCCTCAGATACTGTTAC+TGG - Chr6:49009574-49009593 None:intergenic 45.0%
GCAGTGTTGTCAATTGTGGA+AGG + Chr6:49010146-49010165 MsG0680032762.01.T01:intron 45.0%
GGACATACGAAGTTCGAGAA+GGG + Chr6:49011659-49011678 MsG0680032762.01.T01:CDS 45.0%
GGCACTATAGTGCTATCACA+TGG + Chr6:49010666-49010685 MsG0680032762.01.T01:intron 45.0%
GGTCGGAATCAGAATTTGGT+GGG - Chr6:49010340-49010359 None:intergenic 45.0%
TCCTTGAAATTCCGGGTTCA+CGG - Chr6:49010248-49010267 None:intergenic 45.0%
TCTGTTATGGCTAGACCATG+CGG + Chr6:49011793-49011812 MsG0680032762.01.T01:CDS 45.0%
TGAGTCCACTATGAAAACCG+TGG + Chr6:49010699-49010718 MsG0680032762.01.T01:intron 45.0%
TGGTCGGAATCAGAATTTGG+TGG - Chr6:49010341-49010360 None:intergenic 45.0%
TTTCGCTATCAGGACAGTAG+CGG + Chr6:49010733-49010752 MsG0680032762.01.T01:intron 45.0%
! ACTGTCCTGATAGCGAAACT+GGG - Chr6:49010731-49010750 None:intergenic 45.0%
! AGAGGAAAATCGGGAAGTAC+CGG - Chr6:49011534-49011553 None:intergenic 45.0%
! AGGGATGACTTTGAGAGGAT+TGG + Chr6:49011287-49011306 MsG0680032762.01.T01:intron 45.0%
! CGTATGATTTTGCTACCCCT+TGG - Chr6:49010376-49010395 None:intergenic 45.0%
! GAATCAGAATTTGGTGGGTG+CGG - Chr6:49010335-49010354 None:intergenic 45.0%
! GGTTTTCCTGAAGAGTTTGC+TGG + Chr6:49011356-49011375 MsG0680032762.01.T01:CDS 45.0%
! TACTGTCCTGATAGCGAAAC+TGG - Chr6:49010732-49010751 None:intergenic 45.0%
! TTGAATCTGGCACTGGAAGT+GGG + Chr6:49009812-49009831 MsG0680032762.01.T01:CDS 45.0%
! TTTGACTTCCTTGGCCATGA+CGG + Chr6:49010418-49010437 MsG0680032762.01.T01:intron 45.0%
!! GACGGTAATGATGGCTCTAA+TGG + Chr6:49011703-49011722 MsG0680032762.01.T01:CDS 45.0%
!! TTTTTGGTGAAACTGGGTCC+TGG + Chr6:49010215-49010234 MsG0680032762.01.T01:intron 45.0%
!!! GATCGCGATTTTGACTTCCT+TGG + Chr6:49010409-49010428 MsG0680032762.01.T01:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG + Chr6:49011638-49011657 MsG0680032762.01.T01:CDS 45.0%
!!! TTTCTCTGTTTTCGGGTTGC+AGG + Chr6:49009436-49009455 MsG0680032762.01.T01:CDS 45.0%
AAATTCCGGGTTCACGGGTT+GGG - Chr6:49010242-49010261 None:intergenic 50.0%
AAGAGGAGGCAACGTTCAGA+TGG + Chr6:49011739-49011758 MsG0680032762.01.T01:CDS 50.0%
ACCATACTCCAGCATGCCAA+GGG + Chr6:49010357-49010376 MsG0680032762.01.T01:intron 50.0%
AGTGAGGGACATTCAAGGTG+AGG + Chr6:49011334-49011353 MsG0680032762.01.T01:CDS 50.0%
CCTTGAAATTCCGGGTTCAC+GGG - Chr6:49010247-49010266 None:intergenic 50.0%
CGGACATACGAAGTTCGAGA+AGG + Chr6:49011658-49011677 MsG0680032762.01.T01:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG - Chr6:49011657-49011676 None:intergenic 50.0%
GATTCAAGAACGACGCAACC+AGG - Chr6:49009799-49009818 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG + Chr6:49010535-49010554 MsG0680032762.01.T01:intron 50.0%
GCAGAAAATTGCAGGTACGG+CGG - Chr6:49010301-49010320 None:intergenic 50.0%
GCTATCCCAGTTTCGCTATC+AGG + Chr6:49010723-49010742 MsG0680032762.01.T01:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG - Chr6:49011416-49011435 None:intergenic 50.0%
GGAATAGCTAGCCAAGGTTG+AGG - Chr6:49011410-49011429 None:intergenic 50.0%
GGATCACCGCAGAAAATTGC+AGG - Chr6:49010309-49010328 None:intergenic 50.0%
GGTGAAGCTAGAGTTCACAC+TGG + Chr6:49011814-49011833 MsG0680032762.01.T01:CDS 50.0%
GGTTGCGTCGTTCTTGAATC+TGG + Chr6:49009799-49009818 MsG0680032762.01.T01:CDS 50.0%
GTCGTTCTTGAATCTGGCAC+TGG + Chr6:49009805-49009824 MsG0680032762.01.T01:CDS 50.0%
GTGAGGGACATTCAAGGTGA+GGG + Chr6:49011335-49011354 MsG0680032762.01.T01:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG + Chr6:49011725-49011744 MsG0680032762.01.T01:CDS 50.0%
TTATGAAGGGATGCCCGTCA+TGG - Chr6:49010435-49010454 None:intergenic 50.0%
TTGACTTCCTTGGCCATGAC+GGG + Chr6:49010419-49010438 MsG0680032762.01.T01:intron 50.0%
TTGGCATGCTGGAGTATGGT+CGG - Chr6:49010357-49010376 None:intergenic 50.0%
! CTTGAATCTGGCACTGGAAG+TGG + Chr6:49009811-49009830 MsG0680032762.01.T01:CDS 50.0%
! GCAATTTTCTGCGGTGATCC+TGG + Chr6:49010309-49010328 MsG0680032762.01.T01:intron 50.0%
! GCCGTACCTGCAATTTTCTG+CGG + Chr6:49010300-49010319 MsG0680032762.01.T01:intron 50.0%
! TAGCGCCACGGTTTTCATAG+TGG - Chr6:49010707-49010726 None:intergenic 50.0%
! TGAACTCTAGCTTCACCGCA+TGG - Chr6:49011811-49011830 None:intergenic 50.0%
!! TCCTGAAGAGTTTGCTGGCT+TGG + Chr6:49011361-49011380 MsG0680032762.01.T01:CDS 50.0%
ACCGTGGCCACCATTTGTGA+AGG + Chr6:49010496-49010515 MsG0680032762.01.T01:intron 55.0%
ACGTTCAGATGGAGCTAGCG+TGG + Chr6:49011750-49011769 MsG0680032762.01.T01:CDS 55.0%
ACTTAGTCACTGCGGGAGTG+AGG + Chr6:49011318-49011337 MsG0680032762.01.T01:CDS 55.0%
ATCTGCATGTGTGCCCAACG+TGG + Chr6:49010839-49010858 MsG0680032762.01.T01:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG + Chr6:49010358-49010377 MsG0680032762.01.T01:intron 55.0%
CCCGTGAACCCGGAATTTCA+AGG + Chr6:49010244-49010263 MsG0680032762.01.T01:intron 55.0%
CCCTTCACAAATGGTGGCCA+CGG - Chr6:49010500-49010519 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG + Chr6:49010497-49010516 MsG0680032762.01.T01:intron 55.0%
CTGATCCAGCCCTATGCTCA+TGG - Chr6:49010543-49010562 None:intergenic 55.0%
CTTAGTCACTGCGGGAGTGA+GGG + Chr6:49011319-49011338 MsG0680032762.01.T01:CDS 55.0%
GAAATTCCGGGTTCACGGGT+TGG - Chr6:49010243-49010262 None:intergenic 55.0%
GAACGTTGCCTCCTCTTGCA+AGG - Chr6:49011736-49011755 None:intergenic 55.0%
GACCATACTCCAGCATGCCA+AGG + Chr6:49010356-49010375 MsG0680032762.01.T01:intron 55.0%
GCCAAGCCAGCAAACTCTTC+AGG - Chr6:49011365-49011384 None:intergenic 55.0%
TCTGCCTCACTATTCCACCG+TGG + Chr6:49010480-49010499 MsG0680032762.01.T01:intron 55.0%
TTCACAAATGGTGGCCACGG+TGG - Chr6:49010497-49010516 None:intergenic 55.0%
TTTGCTACCCCTTGGCATGC+TGG - Chr6:49010368-49010387 None:intergenic 55.0%
!! TCGGGAAGTACCGGTGAAGT+TGG - Chr6:49011525-49011544 None:intergenic 55.0%
AGTCAGGTGCAGCACCTAGC+TGG - Chr6:49010581-49010600 None:intergenic 60.0%
CCCCTTGGCATGCTGGAGTA+TGG - Chr6:49010361-49010380 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG + Chr6:49009853-49009872 MsG0680032762.01.T01:CDS 60.0%
GCGAAACTGGGATAGCGCCA+CGG - Chr6:49010719-49010738 None:intergenic 60.0%
GCGGGAGTGAGGGACATTCA+AGG + Chr6:49011329-49011348 MsG0680032762.01.T01:CDS 60.0%
GTGGCCACGGTGGAATAGTG+AGG - Chr6:49010487-49010506 None:intergenic 60.0%
! AGAATTTGGTGGGTGCGGCC+AGG - Chr6:49010330-49010349 None:intergenic 60.0%
AGGGATGCCCGTCATGGCCA+AGG - Chr6:49010429-49010448 None:intergenic 65.0%
CTGGACCCAACCCGTGAACC+CGG + Chr6:49010234-49010253 MsG0680032762.01.T01:intron 65.0%
!! CGGGTTCACGGGTTGGGTCC+AGG - Chr6:49010236-49010255 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 49009396 49012116 49009396 ID=MsG0680032762.01;Name=MsG0680032762.01
Chr6 mRNA 49009396 49012116 49009396 ID=MsG0680032762.01.T01;Parent=MsG0680032762.01;Name=MsG0680032762.01.T01;_AED=0.36;_eAED=0.36;_QI=28|1|1|1|1|1|3|252|325
Chr6 exon 49009396 49009923 49009396 ID=MsG0680032762.01.T01:exon:17010;Parent=MsG0680032762.01.T01
Chr6 exon 49011289 49011533 49011289 ID=MsG0680032762.01.T01:exon:17011;Parent=MsG0680032762.01.T01
Chr6 exon 49011632 49012116 49011632 ID=MsG0680032762.01.T01:exon:17012;Parent=MsG0680032762.01.T01
Chr6 five_prime_UTR 49009396 49009423 49009396 ID=MsG0680032762.01.T01:five_prime_utr;Parent=MsG0680032762.01.T01
Chr6 CDS 49009424 49009923 49009424 ID=MsG0680032762.01.T01:cds;Parent=MsG0680032762.01.T01
Chr6 CDS 49011289 49011533 49011289 ID=MsG0680032762.01.T01:cds;Parent=MsG0680032762.01.T01
Chr6 CDS 49011632 49011864 49011632 ID=MsG0680032762.01.T01:cds;Parent=MsG0680032762.01.T01
Chr6 three_prime_UTR 49011865 49012116 49011865 ID=MsG0680032762.01.T01:three_prime_utr;Parent=MsG0680032762.01.T01
Gene Sequence

>MsG0680032762.01.T01

ATGTTTCATTGTTTTCTCTGTTTTCGGGTTGCAGGTACTTGTTTATTGAAAATGTTGGCTATCGATTCTACAAGAAAGATATCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGAAAGGCATGATAGCATGAAGCCAGTAACAGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTTAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAACAATAAGGGCGGTTTTGTTTACTTATTAGCTCCTACTCCTGAATTATGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATATTGCAGATATCAGCTTCATTGTTATGTATTTGGAGATTGTTCCTGGTTGCGTCGTTCTTGAATCTGGCACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGGATGACTTTGAGAGGATTGGTATAAGCAACTTAGTCACTGCGGGAGTGAGGGACATTCAAGGTGAGGGTTTTCCTGAAGAGTTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGACATGACGGCACTTTATGTTCTTTCTCCCCGTGTATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACCGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTCAGAGATTGACGGTAATGATGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAACGTTCAGATGGAGCTAGCGTGGTGAGCTATCCCAATTCTTCTTCTGTTATGGCTAGACCATGCGGTGAAGCTAGAGTTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>MsG0680032762.01.T01

MFHCFLCFRVAGTCLLKMLAIDSTRKISFNRTISNGDLAIVYERHDSMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFNNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFERIGISNLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESSEIDGNDGSNGSLPCKRRQRSDGASVVSYPNSSSVMARPCGEARVHTGYLTFARLN*