AlfalfaGEDB | MsG0580025268.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025268.01

KEGG: -
GO: GO:0005515
PFAM: PF02201

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025268.01.T01	XP_003612484.1	97.744	133	3	0	1	133	1	133	2.84E-89	265

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025268.01.T01	G7K410	97.744	133	3	0	1	133	1	133	1.36e-89	265

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047791.01	MsG0580025268.01	0.830158	3.438105e-55	3.593155e-52
MsG0080048152.01	MsG0580025268.01	0.833404	5.437798e-56	6.256043e-53
MsG0080048197.01	MsG0580025268.01	0.804349	2.254309e-49	1.159673e-46
MsG0080048383.01	MsG0580025268.01	0.837858	4.056556e-57	5.338359e-54
MsG0080048687.01	MsG0580025268.01	0.828751	7.552857e-55	7.575181e-52
MsG0080048784.01	MsG0580025268.01	0.836825	7.457372e-57	9.507793e-54
MsG0080049135.01	MsG0580025268.01	0.821977	3.030898e-53	2.503540e-50
MsG0180000139.01	MsG0580025268.01	0.813192	2.899089e-51	1.880493e-48
MsG0180000144.01	MsG0580025268.01	0.806574	7.700821e-50	4.194260e-47
MsG0180000155.01	MsG0580025268.01	0.800249	1.574551e-48	7.296517e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025268.01.T01	MTR_5g025520	97.744	133	3	0	1	133	1	133	3.44e-93	265
MsG0580025268.01.T01	MTR_5g025500	37.662	77	48	0	26	102	54	130	1.46e-11	58.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 55 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCGTCGCCGAAATCAT+GGG	0.576114	5:-16213574	MsG0580025268.01.T01:CDS
AAATAGGAAGTGGAGGCATG+TGG	0.631407	5:-16212277	MsG0580025268.01.T01:exon
AATATTGTCATTGGACCACA+AGG	0.613637	5:+16212106	None:intergenic
AATTTGCAAACACTGTTGCG+TGG	0.632781	5:-16212442	MsG0580025268.01.T01:CDS
ACTGGTAGCTTTGCGTGTCT+TGG	0.404946	5:+16213540	None:intergenic
ACTGTAGAGTTTGATGAACT+TGG	0.446589	5:+16213456	None:intergenic
AGCAGAAGGATGAATCCGGC+GGG	0.612519	5:-16213631	MsG0580025268.01.T01:exon
AGCTTGTCTTTCTTGATACC+AGG	0.515565	5:+16212475	None:intergenic
AGGAAGTGGAGGCATGTGGT+TGG	0.560814	5:-16212273	MsG0580025268.01.T01:three_prime_UTR
AGGGCGAAGGAGCATGTCGA+CGG	0.655015	5:-16213602	MsG0580025268.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAAAGACTAATCAGTAGG+GGG	+	Chr5:16212574-16212593	None:intergenic	30.0%
CTGCATGATAATTTCATGAA+TGG	-	Chr5:16213570-16213589	MsG0580025268.01.T01:CDS	30.0%
GAAGAAAGGGAAAAAGAAAT+AGG	-	Chr5:16213455-16213474	MsG0580025268.01.T01:CDS	30.0%
GATTAAAGACTAATCAGTAG+GGG	+	Chr5:16212575-16212594	None:intergenic	30.0%
GCTTATTTGGAAATGGTATA+TGG	-	Chr5:16213237-16213256	MsG0580025268.01.T01:intron	30.0%
TATCAAGAAAGACAAGCTTT+GGG	-	Chr5:16213278-16213297	MsG0580025268.01.T01:intron	30.0%
TCTGAAAAACATGGAAATGA+TGG	+	Chr5:16212404-16212423	None:intergenic	30.0%
TTGAGAGAACTTATGAAAAC+AGG	-	Chr5:16212849-16212868	MsG0580025268.01.T01:intron	30.0%
TTTGATGAACTTGGAAATGA+TGG	+	Chr5:16212286-16212305	None:intergenic	30.0%
AAATGGGAAGAATTGAAACC+CGG	+	Chr5:16212320-16212339	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	16212095	16213675	16212095	ID=MsG0580025268.01;Name=MsG0580025268.01
Chr5	mRNA	16212095	16213675	16212095	ID=MsG0580025268.01.T01;Parent=MsG0580025268.01;Name=MsG0580025268.01.T01;_AED=0.44;_eAED=0.44;_QI=31\|1\|1\|1\|1\|1\|2\|199\|133
Chr5	exon	16212095	16212500	16212095	ID=MsG0580025268.01.T01:exon:20585;Parent=MsG0580025268.01.T01
Chr5	exon	16213450	16213675	16213450	ID=MsG0580025268.01.T01:exon:20584;Parent=MsG0580025268.01.T01
Chr5	five_prime_UTR	16213645	16213675	16213645	ID=MsG0580025268.01.T01:five_prime_utr;Parent=MsG0580025268.01.T01
Chr5	CDS	16213450	16213644	16213450	ID=MsG0580025268.01.T01:cds;Parent=MsG0580025268.01.T01
Chr5	CDS	16212294	16212500	16212294	ID=MsG0580025268.01.T01:cds;Parent=MsG0580025268.01.T01
Chr5	three_prime_UTR	16212095	16212293	16212095	ID=MsG0580025268.01.T01:three_prime_utr;Parent=MsG0580025268.01.T01

Gene Sequence

>MsG0580025268.01.T01

ATGAATCCGGCGGGTTTAACAGGGCGAAGGAGCATGTCGACGGTATCCAAAGCCGTCGCCGAAATCATGGGTGTCGGCAAAACCAAGACACGCAAAGCTACCAGTGAACTCTGCACTTTCATGGGCATTCCTCATCAATCTCGTTCCGAAATTGCTTCCATCATTTCCAAGTTCATCAAACTCTACAGTTTCCGGAGTCCTGGTATCAAGAAAGACAAGCTTTGGGAGCAGAATTTGCAAACACTGTTGCGTGGCAGAAACAGCGTTGGTTTTCCTGAGATTGCTAAGATTCTGTCTCCGGAATTCAGCCAGGGTGCGATTAATATCAAGGATACTAATACGGATTCTTCCGCGGATAACACAAAGGGGAAAGGCCAACAGAAGAAAGGGAAAAAGAAATAG

Protein sequence

>MsG0580025268.01.T01

MNPAGLTGRRSMSTVSKAVAEIMGVGKTKTRKATSELCTFMGIPHQSRSEIASIISKFIKLYSFRSPGIKKDKLWEQNLQTLLRGRNSVGFPEIAKILSPEFSQGAINIKDTNTDSSADNTKGKGQQKKGKKK*