AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025268.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025268.01.T01 MTR_5g025520 97.744 133 3 0 1 133 1 133 3.44e-93 265
MsG0580025268.01.T01 MTR_5g025500 37.662 77 48 0 26 102 54 130 1.46e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCAAACTCTACAGTTTC+CGG 0.200149 5:-16213450 MsG0580025268.01.T01:CDS
ATGAATCCGGCGGGTTTAAC+AGG 0.280966 5:-16213622 MsG0580025268.01.T01:CDS
GGAAATGATGGAAGCAATTT+CGG 0.295914 5:+16213477 None:intergenic
TTTGAGTATTGAAACTAGTT+TGG 0.342271 5:-16212135 MsG0580025268.01.T01:three_prime_UTR
ATCTAACTTGGTTCCTATCT+AGG 0.344574 5:+16212238 None:intergenic
CGCCGGATTCATCCTTCTGC+TGG 0.358107 5:+16213633 None:intergenic
CATGATTTCGGCGACGGCTT+TGG 0.360532 5:+16213576 None:intergenic
CAGTGAACTCTGCACTTTCA+TGG 0.368977 5:-16213521 MsG0580025268.01.T01:CDS
CTGCATGATAATTTCATGAA+TGG 0.375196 5:-16212178 MsG0580025268.01.T01:three_prime_UTR
CATCAAACTCTACAGTTTCC+GGG 0.376717 5:-16213449 MsG0580025268.01.T01:intron
ATCGCACCCTGGCTGAATTC+CGG 0.377231 5:+16212376 None:intergenic
TTTGCCGACACCCATGATTT+CGG 0.381159 5:+16213564 None:intergenic
TTTGATGAACTTGGAAATGA+TGG 0.402949 5:+16213465 None:intergenic
ACTGGTAGCTTTGCGTGTCT+TGG 0.404946 5:+16213540 None:intergenic
CAAAGCCGTCGCCGAAATCA+TGG 0.409452 5:-16213575 MsG0580025268.01.T01:CDS
CAGAAAATGAATATTGTCAT+TGG 0.428328 5:+16212097 None:intergenic
GTATCAAGAAAGACAAGCTT+TGG 0.428352 5:-16212471 MsG0580025268.01.T01:CDS
ACTGTAGAGTTTGATGAACT+TGG 0.446589 5:+16213456 None:intergenic
GGGAAAGGCCAACAGAAGAA+AGG 0.464992 5:-16212307 MsG0580025268.01.T01:CDS
GACAGAATCTTAGCAATCTC+AGG 0.466374 5:+16212400 None:intergenic
TAATATCAAGGATACTAATA+CGG 0.503603 5:-16212353 MsG0580025268.01.T01:CDS
TCTGTCTCCGGAATTCAGCC+AGG 0.504471 5:-16212383 MsG0580025268.01.T01:CDS
GACACCCATGATTTCGGCGA+CGG 0.507275 5:+16213570 None:intergenic
GGAAAGGCCAACAGAAGAAA+GGG 0.508550 5:-16212306 MsG0580025268.01.T01:CDS
GATTGCTAAGATTCTGTCTC+CGG 0.511219 5:-16212395 MsG0580025268.01.T01:CDS
AGTGAACTCTGCACTTTCAT+GGG 0.512210 5:-16213520 MsG0580025268.01.T01:CDS
AGCTTGTCTTTCTTGATACC+AGG 0.515565 5:+16212475 None:intergenic
TATGGTGTTGTGCAGAGTCC+TGG 0.516143 5:-16212493 MsG0580025268.01.T01:intron
CCAGGGTGCGATTAATATCA+AGG 0.519238 5:-16212365 MsG0580025268.01.T01:CDS
CATGAAAGTGCAGAGTTCAC+TGG 0.524418 5:+16213522 None:intergenic
ATCCAGCAGAAGGATGAATC+CGG 0.526821 5:-16213635 MsG0580025268.01.T01:exon
CTTCGCCCTGTTAAACCCGC+CGG 0.548257 5:+16213616 None:intergenic
TATCAAGAAAGACAAGCTTT+GGG 0.550356 5:-16212470 MsG0580025268.01.T01:CDS
AGGAAGTGGAGGCATGTGGT+TGG 0.560814 5:-16212273 MsG0580025268.01.T01:three_prime_UTR
TCTTCCGCGGATAACACAAA+GGG 0.561908 5:-16212328 MsG0580025268.01.T01:CDS
AAAGCCGTCGCCGAAATCAT+GGG 0.576114 5:-16213574 MsG0580025268.01.T01:CDS
GCGGATAACACAAAGGGGAA+AGG 0.582569 5:-16212322 MsG0580025268.01.T01:CDS
CTAGTTTGGCTATTGCCTTG+TGG 0.596708 5:-16212121 MsG0580025268.01.T01:three_prime_UTR
TTCTTCCGCGGATAACACAA+AGG 0.602214 5:-16212329 MsG0580025268.01.T01:CDS
CGGCGGGTTTAACAGGGCGA+AGG 0.602549 5:-16213615 MsG0580025268.01.T01:CDS
TGAATCCGGCGGGTTTAACA+GGG 0.604481 5:-16213621 MsG0580025268.01.T01:CDS
GTCGCCGAAATCATGGGTGT+CGG 0.610089 5:-16213568 MsG0580025268.01.T01:CDS
AGCAGAAGGATGAATCCGGC+GGG 0.612519 5:-16213631 MsG0580025268.01.T01:exon
AATATTGTCATTGGACCACA+AGG 0.613637 5:+16212106 None:intergenic
AAATAGGAAGTGGAGGCATG+TGG 0.631407 5:-16212277 MsG0580025268.01.T01:exon
AATTTGCAAACACTGTTGCG+TGG 0.632781 5:-16212442 MsG0580025268.01.T01:CDS
TTGCGTGGCAGAAACAGCGT+TGG 0.639499 5:-16212427 MsG0580025268.01.T01:CDS
ATTTCGGAACGAGATTGATG+AGG 0.642540 5:+16213493 None:intergenic
CTGTCTCCGGAATTCAGCCA+GGG 0.642587 5:-16212382 MsG0580025268.01.T01:CDS
AGGGCGAAGGAGCATGTCGA+CGG 0.655015 5:-16213602 MsG0580025268.01.T01:CDS
CAGCAGAAGGATGAATCCGG+CGG 0.656372 5:-16213632 MsG0580025268.01.T01:exon
TACTAATACGGATTCTTCCG+CGG 0.657291 5:-16212341 MsG0580025268.01.T01:CDS
CCTTGATATTAATCGCACCC+TGG 0.662781 5:+16212365 None:intergenic
TTTCCCCTTTGTGTTATCCG+CGG 0.666626 5:+16212324 None:intergenic
CTTCCGCGGATAACACAAAG+GGG 0.722706 5:-16212327 MsG0580025268.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAATAAAAGATAAAATA+AGG + Chr5:16213075-16213094 None:intergenic 10.0%
!! AATGAAACAAAAAGAAAAAT+GGG + Chr5:16212336-16212355 None:intergenic 15.0%
!! TAATGAAACAAAAAGAAAAA+TGG + Chr5:16212337-16212356 None:intergenic 15.0%
!!! TTTATTTACTTTTGCTTATT+TGG - Chr5:16213224-16213243 MsG0580025268.01.T01:intron 15.0%
!! AAAGTTTCAAAAATCTAACT+TGG + Chr5:16213525-16213544 None:intergenic 20.0%
!! ATTAAGCTGTTTATAAAAAC+AGG - Chr5:16213018-16213037 MsG0580025268.01.T01:intron 20.0%
!! CTTTCCATAAAATAAGATAT+AGG + Chr5:16213045-16213064 None:intergenic 20.0%
!! TAATATCAAGGATACTAATA+CGG - Chr5:16213395-16213414 MsG0580025268.01.T01:intron 20.0%
!! TCAAATAAACCAATTCAAAT+AGG + Chr5:16212704-16212723 None:intergenic 20.0%
!! TTAAGCTGTTTATAAAAACA+GGG - Chr5:16213019-16213038 MsG0580025268.01.T01:intron 20.0%
!!! TAAGTTTGTTTCTATAACAA+AGG + Chr5:16212984-16213003 None:intergenic 20.0%
! AAGATTAAAGACTAATCAGT+AGG + Chr5:16212577-16212596 None:intergenic 25.0%
! AGATTAAAGACTAATCAGTA+GGG + Chr5:16212576-16212595 None:intergenic 25.0%
! CAGAAAATGAATATTGTCAT+TGG + Chr5:16213654-16213673 None:intergenic 25.0%
! TTGAAAATAATCACTAGTCA+GGG + Chr5:16212663-16212682 None:intergenic 25.0%
! TTTGAAAATAATCACTAGTC+AGG + Chr5:16212664-16212683 None:intergenic 25.0%
!! TAAACTATCTCTGAAAAACA+TGG + Chr5:16212413-16212432 None:intergenic 25.0%
!! TACTTTTGCTTATTTGGAAA+TGG - Chr5:16213230-16213249 MsG0580025268.01.T01:intron 25.0%
!! TTTTCATAAGTTCTCTCAAA+CGG + Chr5:16212848-16212867 None:intergenic 25.0%
!!! GTTTTTTTCTATTCCTAGAT+AGG - Chr5:16213497-16213516 MsG0580025268.01.T01:CDS 25.0%
!!! TTTGAGTATTGAAACTAGTT+TGG - Chr5:16213613-16213632 MsG0580025268.01.T01:CDS 25.0%
ATTAAAGACTAATCAGTAGG+GGG + Chr5:16212574-16212593 None:intergenic 30.0%
CTGCATGATAATTTCATGAA+TGG - Chr5:16213570-16213589 MsG0580025268.01.T01:CDS 30.0%
GAAGAAAGGGAAAAAGAAAT+AGG - Chr5:16213455-16213474 MsG0580025268.01.T01:CDS 30.0%
GATTAAAGACTAATCAGTAG+GGG + Chr5:16212575-16212594 None:intergenic 30.0%
GCTTATTTGGAAATGGTATA+TGG - Chr5:16213237-16213256 MsG0580025268.01.T01:intron 30.0%
TATCAAGAAAGACAAGCTTT+GGG - Chr5:16213278-16213297 MsG0580025268.01.T01:intron 30.0%
TCTGAAAAACATGGAAATGA+TGG + Chr5:16212404-16212423 None:intergenic 30.0%
TTGAGAGAACTTATGAAAAC+AGG - Chr5:16212849-16212868 MsG0580025268.01.T01:intron 30.0%
TTTGATGAACTTGGAAATGA+TGG + Chr5:16212286-16212305 None:intergenic 30.0%
!!! AGGGCCTATATCTTATTTTA+TGG - Chr5:16213038-16213057 MsG0580025268.01.T01:intron 30.0%
AAATGGGAAGAATTGAAACC+CGG + Chr5:16212320-16212339 None:intergenic 35.0%
AACCAATTCAAATAGGCTCA+TGG + Chr5:16212697-16212716 None:intergenic 35.0%
AACCATGAGCCTATTTGAAT+TGG - Chr5:16212692-16212711 MsG0580025268.01.T01:intron 35.0%
AATATTGTCATTGGACCACA+AGG + Chr5:16213645-16213664 None:intergenic 35.0%
ACTGTAGAGTTTGATGAACT+TGG + Chr5:16212295-16212314 None:intergenic 35.0%
AGGGAAAAAGAAATAGGAAG+TGG - Chr5:16213461-16213480 MsG0580025268.01.T01:CDS 35.0%
ATCTAACTTGGTTCCTATCT+AGG + Chr5:16213513-16213532 None:intergenic 35.0%
CAAGTGCTTGTTTCAATACA+TGG + Chr5:16212733-16212752 None:intergenic 35.0%
GAAAAAGAAATAGGAAGTGG+AGG - Chr5:16213464-16213483 MsG0580025268.01.T01:CDS 35.0%
GAGAACATTGATCAAACAGT+TGG + Chr5:16212367-16212386 None:intergenic 35.0%
GGATAAACAACTTCATTGAG+TGG + Chr5:16213149-16213168 None:intergenic 35.0%
GTATCAAGAAAGACAAGCTT+TGG - Chr5:16213277-16213296 MsG0580025268.01.T01:intron 35.0%
TCATCAAACTCTACAGTTTC+CGG - Chr5:16212298-16212317 MsG0580025268.01.T01:CDS 35.0%
! AGCGATAAGCTCTTTTCATA+AGG + Chr5:16212932-16212951 None:intergenic 35.0%
! GGAAATGATGGAAGCAATTT+CGG + Chr5:16212274-16212293 None:intergenic 35.0%
! TCTTTTTCCCTTTCTTCTGT+TGG + Chr5:16213452-16213471 None:intergenic 35.0%
AATTTGCAAACACTGTTGCG+TGG - Chr5:16213306-16213325 MsG0580025268.01.T01:intron 40.0%
AGCTTGTCTTTCTTGATACC+AGG + Chr5:16213276-16213295 None:intergenic 40.0%
AGTCCCTATCAGTGATTAAG+TGG + Chr5:16212619-16212638 None:intergenic 40.0%
AGTGAACTCTGCACTTTCAT+GGG - Chr5:16212228-16212247 MsG0580025268.01.T01:three_prime_UTR 40.0%
ATTTCGGAACGAGATTGATG+AGG + Chr5:16212258-16212277 None:intergenic 40.0%
CAATGGCAACACTCTTACTT+AGG + Chr5:16213179-16213198 None:intergenic 40.0%
CACCCACTTAATCACTGATA+GGG - Chr5:16212613-16212632 MsG0580025268.01.T01:intron 40.0%
CACTCTTACTTAGGCTCATT+TGG + Chr5:16213170-16213189 None:intergenic 40.0%
CATCAAACTCTACAGTTTCC+GGG - Chr5:16212299-16212318 MsG0580025268.01.T01:CDS 40.0%
CTTTCACTTTAGTCGCTCAA+TGG + Chr5:16213196-16213215 None:intergenic 40.0%
GAAGACAAAAATCCAGCAGA+AGG - Chr5:16212103-16212122 MsG0580025268.01.T01:three_prime_UTR 40.0%
GACAGAATCTTAGCAATCTC+AGG + Chr5:16213351-16213370 None:intergenic 40.0%
GATTGCTAAGATTCTGTCTC+CGG - Chr5:16213353-16213372 MsG0580025268.01.T01:intron 40.0%
GTCCCTATCAGTGATTAAGT+GGG + Chr5:16212618-16212637 None:intergenic 40.0%
TACTAATACGGATTCTTCCG+CGG - Chr5:16213407-16213426 MsG0580025268.01.T01:intron 40.0%
TCACCCACTTAATCACTGAT+AGG - Chr5:16212612-16212631 MsG0580025268.01.T01:intron 40.0%
TTGAGTGGTTATAGCACAAG+CGG + Chr5:16213134-16213153 None:intergenic 40.0%
ATCCAGCAGAAGGATGAATC+CGG - Chr5:16212113-16212132 MsG0580025268.01.T01:three_prime_UTR 45.0%
CAGTGAACTCTGCACTTTCA+TGG - Chr5:16212227-16212246 MsG0580025268.01.T01:three_prime_UTR 45.0%
CCAGGGTGCGATTAATATCA+AGG - Chr5:16213383-16213402 MsG0580025268.01.T01:intron 45.0%
CCTTGATATTAATCGCACCC+TGG + Chr5:16213386-16213405 None:intergenic 45.0%
CTAGTTTGGCTATTGCCTTG+TGG - Chr5:16213627-16213646 MsG0580025268.01.T01:exon 45.0%
GGAAAGGCCAACAGAAGAAA+GGG - Chr5:16213442-16213461 MsG0580025268.01.T01:intron 45.0%
TCTTCCGCGGATAACACAAA+GGG - Chr5:16213420-16213439 MsG0580025268.01.T01:intron 45.0%
TTCTTCCGCGGATAACACAA+AGG - Chr5:16213419-16213438 MsG0580025268.01.T01:intron 45.0%
TTTCCCCTTTGTGTTATCCG+CGG + Chr5:16213427-16213446 None:intergenic 45.0%
TTTGCCGACACCCATGATTT+CGG + Chr5:16212187-16212206 None:intergenic 45.0%
! AAATAGGAAGTGGAGGCATG+TGG - Chr5:16213471-16213490 MsG0580025268.01.T01:CDS 45.0%
! CATGAAAGTGCAGAGTTCAC+TGG + Chr5:16212229-16212248 None:intergenic 45.0%
AAAGCCGTCGCCGAAATCAT+GGG - Chr5:16212174-16212193 MsG0580025268.01.T01:three_prime_UTR 50.0%
CTTCCGCGGATAACACAAAG+GGG - Chr5:16213421-16213440 MsG0580025268.01.T01:intron 50.0%
GCGGATAACACAAAGGGGAA+AGG - Chr5:16213426-16213445 MsG0580025268.01.T01:intron 50.0%
GGGAAAGGCCAACAGAAGAA+AGG - Chr5:16213441-16213460 MsG0580025268.01.T01:intron 50.0%
! ACTGGTAGCTTTGCGTGTCT+TGG + Chr5:16212211-16212230 None:intergenic 50.0%
! ATGAATCCGGCGGGTTTAAC+AGG - Chr5:16212126-16212145 MsG0580025268.01.T01:three_prime_UTR 50.0%
! TGAATCCGGCGGGTTTAACA+GGG - Chr5:16212127-16212146 MsG0580025268.01.T01:three_prime_UTR 50.0%
!! TATGGTGTTGTGCAGAGTCC+TGG - Chr5:16213255-16213274 MsG0580025268.01.T01:intron 50.0%
AGCAGAAGGATGAATCCGGC+GGG - Chr5:16212117-16212136 MsG0580025268.01.T01:three_prime_UTR 55.0%
ATCGCACCCTGGCTGAATTC+CGG + Chr5:16213375-16213394 None:intergenic 55.0%
CAAAGCCGTCGCCGAAATCA+TGG - Chr5:16212173-16212192 MsG0580025268.01.T01:three_prime_UTR 55.0%
CAGCAGAAGGATGAATCCGG+CGG - Chr5:16212116-16212135 MsG0580025268.01.T01:three_prime_UTR 55.0%
CATGATTTCGGCGACGGCTT+TGG + Chr5:16212175-16212194 None:intergenic 55.0%
CTGTCTCCGGAATTCAGCCA+GGG - Chr5:16213366-16213385 MsG0580025268.01.T01:intron 55.0%
GACACCCATGATTTCGGCGA+CGG + Chr5:16212181-16212200 None:intergenic 55.0%
GTCGCCGAAATCATGGGTGT+CGG - Chr5:16212180-16212199 MsG0580025268.01.T01:three_prime_UTR 55.0%
TCTGTCTCCGGAATTCAGCC+AGG - Chr5:16213365-16213384 MsG0580025268.01.T01:intron 55.0%
TTGCGTGGCAGAAACAGCGT+TGG - Chr5:16213321-16213340 MsG0580025268.01.T01:intron 55.0%
! AGGAAGTGGAGGCATGTGGT+TGG - Chr5:16213475-16213494 MsG0580025268.01.T01:CDS 55.0%
CGCCGGATTCATCCTTCTGC+TGG + Chr5:16212118-16212137 None:intergenic 60.0%
CTTCGCCCTGTTAAACCCGC+CGG + Chr5:16212135-16212154 None:intergenic 60.0%
! AGGGCGAAGGAGCATGTCGA+CGG - Chr5:16212146-16212165 MsG0580025268.01.T01:three_prime_UTR 60.0%
! CGGCGGGTTTAACAGGGCGA+AGG - Chr5:16212133-16212152 MsG0580025268.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 16212095 16213675 16212095 ID=MsG0580025268.01;Name=MsG0580025268.01
Chr5 mRNA 16212095 16213675 16212095 ID=MsG0580025268.01.T01;Parent=MsG0580025268.01;Name=MsG0580025268.01.T01;_AED=0.44;_eAED=0.44;_QI=31|1|1|1|1|1|2|199|133
Chr5 exon 16212095 16212500 16212095 ID=MsG0580025268.01.T01:exon:20585;Parent=MsG0580025268.01.T01
Chr5 exon 16213450 16213675 16213450 ID=MsG0580025268.01.T01:exon:20584;Parent=MsG0580025268.01.T01
Chr5 five_prime_UTR 16213645 16213675 16213645 ID=MsG0580025268.01.T01:five_prime_utr;Parent=MsG0580025268.01.T01
Chr5 CDS 16213450 16213644 16213450 ID=MsG0580025268.01.T01:cds;Parent=MsG0580025268.01.T01
Chr5 CDS 16212294 16212500 16212294 ID=MsG0580025268.01.T01:cds;Parent=MsG0580025268.01.T01
Chr5 three_prime_UTR 16212095 16212293 16212095 ID=MsG0580025268.01.T01:three_prime_utr;Parent=MsG0580025268.01.T01
Gene Sequence

>MsG0580025268.01.T01

ATGAATCCGGCGGGTTTAACAGGGCGAAGGAGCATGTCGACGGTATCCAAAGCCGTCGCCGAAATCATGGGTGTCGGCAAAACCAAGACACGCAAAGCTACCAGTGAACTCTGCACTTTCATGGGCATTCCTCATCAATCTCGTTCCGAAATTGCTTCCATCATTTCCAAGTTCATCAAACTCTACAGTTTCCGGAGTCCTGGTATCAAGAAAGACAAGCTTTGGGAGCAGAATTTGCAAACACTGTTGCGTGGCAGAAACAGCGTTGGTTTTCCTGAGATTGCTAAGATTCTGTCTCCGGAATTCAGCCAGGGTGCGATTAATATCAAGGATACTAATACGGATTCTTCCGCGGATAACACAAAGGGGAAAGGCCAACAGAAGAAAGGGAAAAAGAAATAG

Protein sequence

>MsG0580025268.01.T01

MNPAGLTGRRSMSTVSKAVAEIMGVGKTKTRKATSELCTFMGIPHQSRSEIASIISKFIKLYSFRSPGIKKDKLWEQNLQTLLRGRNSVGFPEIAKILSPEFSQGAINIKDTNTDSSADNTKGKGQQKKGKKK*