AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035877.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035877.01.T01 MTR_6g093210 89.888 89 4 1 21 104 107 195 5.32e-44 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATCAAGACCATAGAAAA+TGG 0.246090 6:+113805740 MsG0680035877.01.T01:CDS
GCAGTAGTTCTTCTTCTTCT+TGG 0.263005 6:+113805788 MsG0680035877.01.T01:CDS
AGAGTGGAATAAAAGATTAT+AGG 0.285564 6:+113805812 MsG0680035877.01.T01:CDS
TGGTTTAAGGGAAGATGATA+TGG 0.290571 6:+113805594 MsG0680035877.01.T01:intron
GAGTGGAATAAAAGATTATA+GGG 0.338883 6:+113805813 MsG0680035877.01.T01:CDS
ATGATTTCAACGAAAATAAC+GGG 0.361681 6:+113805857 MsG0680035877.01.T01:CDS
CAGTAGTTCTTCTTCTTCTT+GGG 0.368246 6:+113805789 MsG0680035877.01.T01:CDS
CAGAAGCTATTATAGATGTA+AGG 0.392130 6:+113805698 MsG0680035877.01.T01:CDS
AAAATGGGAAGACTGGCTTT+TGG 0.418846 6:+113805756 MsG0680035877.01.T01:CDS
TAAGGGAAGCACAAGAAGAT+AGG 0.453317 6:+113805716 MsG0680035877.01.T01:CDS
TCTTCTTCTTCTTGGGAGAG+TGG 0.463042 6:+113805796 MsG0680035877.01.T01:CDS
ACCATAGAAAATGGGAAGAC+TGG 0.481141 6:+113805749 MsG0680035877.01.T01:CDS
GATGATTTCAACGAAAATAA+CGG 0.482770 6:+113805856 MsG0680035877.01.T01:CDS
GAATCAAGACCATAGAAAAT+GGG 0.490106 6:+113805741 MsG0680035877.01.T01:CDS
GGAAGATGATATGGTTGAAG+AGG 0.505252 6:+113805603 MsG0680035877.01.T01:exon
AGAAGCTATTATAGATGTAA+GGG 0.515323 6:+113805699 MsG0680035877.01.T01:CDS
TGGAATAAAAGATTATAGGG+AGG 0.565202 6:+113805816 MsG0680035877.01.T01:CDS
AAGAAGAAAAGTTCGAAGCA+TGG 0.567823 6:+113805665 MsG0680035877.01.T01:CDS
AGTGAGACTTAATGAAGCAT+CGG 0.591789 6:+113804004 MsG0680035877.01.T01:five_prime_UTR
AGAGGATGTTGAATTTGAAG+TGG 0.607061 6:+113805621 MsG0680035877.01.T01:CDS
TAGGGAGGAGTTACGAGGCG+AGG 0.650202 6:+113805831 MsG0680035877.01.T01:CDS
GATTATAGGGAGGAGTTACG+AGG 0.665569 6:+113805826 MsG0680035877.01.T01:CDS
TGATTTCAACGAAAATAACG+GGG 0.743389 6:+113805858 MsG0680035877.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAAAAATAAAATTGAAAT+TGG + Chr6:113804820-113804839 MsG0680035877.01.T01:intron 10.0%
!! TAAATATATATTATATCTAC+CGG + Chr6:113804644-113804663 MsG0680035877.01.T01:intron 10.0%
!!! TTTCAATTTTATTTTTATCA+CGG - Chr6:113804819-113804838 None:intergenic 10.0%
!! TATAAAAATGAAAACAATAG+AGG - Chr6:113804417-113804436 None:intergenic 15.0%
!! TTTAAATAAGATGAAATTGT+TGG - Chr6:113805386-113805405 None:intergenic 15.0%
!!! ATAAACAAATTTGACTTTTT+AGG - Chr6:113805017-113805036 None:intergenic 15.0%
!!! TTTTTTTTTCATTATACAGA+TGG + Chr6:113803980-113803999 MsG0680035877.01.T01:five_prime_UTR 15.0%
!! AAAAAAGAGACATTTAATCT+TGG + Chr6:113805154-113805173 MsG0680035877.01.T01:intron 20.0%
!! AATTAGACTATTTGACAAAA+AGG + Chr6:113805131-113805150 MsG0680035877.01.T01:intron 20.0%
!! ATAGAGGAAATTACATTTAA+CGG - Chr6:113804401-113804420 None:intergenic 20.0%
!!! AAATTTTTTCAACAAGATGA+TGG + Chr6:113805562-113805581 MsG0680035877.01.T01:intron 20.0%
!!! ATTTTCTTTTTTATCTAGAG+TGG - Chr6:113804951-113804970 None:intergenic 20.0%
!!! TACTGTTTTTCTTGTTTTTA+TGG - Chr6:113805528-113805547 None:intergenic 20.0%
!!! TCTGTTAAGTATTTGATTTT+TGG + Chr6:113805889-113805908 MsG0680035877.01.T01:CDS 20.0%
!!! TTTCTTGTTTTTATGGTTTT+TGG - Chr6:113805521-113805540 None:intergenic 20.0%
! AAAATGTGAAGGACAAAAAT+AGG - Chr6:113804774-113804793 None:intergenic 25.0%
! AGAAGCTATTATAGATGTAA+GGG + Chr6:113805699-113805718 MsG0680035877.01.T01:CDS 25.0%
! AGAGTGGAATAAAAGATTAT+AGG + Chr6:113805812-113805831 MsG0680035877.01.T01:CDS 25.0%
! ATAAGTAGTTTCAGAAATGT+GGG + Chr6:113805276-113805295 MsG0680035877.01.T01:intron 25.0%
! ATGATGATAATACAAGTTTG+TGG - Chr6:113805414-113805433 None:intergenic 25.0%
! ATGATTTCAACGAAAATAAC+GGG + Chr6:113805857-113805876 MsG0680035877.01.T01:CDS 25.0%
! ATTGTGTGTACTATTATTTC+TGG + Chr6:113804215-113804234 MsG0680035877.01.T01:intron 25.0%
! GAGTGGAATAAAAGATTATA+GGG + Chr6:113805813-113805832 MsG0680035877.01.T01:CDS 25.0%
! GATGATTTCAACGAAAATAA+CGG + Chr6:113805856-113805875 MsG0680035877.01.T01:CDS 25.0%
! TATAAGTAGTTTCAGAAATG+TGG + Chr6:113805275-113805294 MsG0680035877.01.T01:intron 25.0%
!!! GTTGTTGATAATAGATGAAA+TGG + Chr6:113805238-113805257 MsG0680035877.01.T01:intron 25.0%
!!! TTTTGAAATGAAAGATGTTG+AGG - Chr6:113805469-113805488 None:intergenic 25.0%
AAATGAAGGTAGTAAAACGT+TGG - Chr6:113805492-113805511 None:intergenic 30.0%
AAGAACTCAAAGATAGATAG+TGG + Chr6:113804114-113804133 MsG0680035877.01.T01:intron 30.0%
AGAATCAAGACCATAGAAAA+TGG + Chr6:113805740-113805759 MsG0680035877.01.T01:CDS 30.0%
CAGAAGCTATTATAGATGTA+AGG + Chr6:113805698-113805717 MsG0680035877.01.T01:CDS 30.0%
CGGATCTTACCTAAAATATT+GGG + Chr6:113804484-113804503 MsG0680035877.01.T01:intron 30.0%
GAAATCAATGTCACAATGTT+AGG - Chr6:113804317-113804336 None:intergenic 30.0%
GAATCAAGACCATAGAAAAT+GGG + Chr6:113805741-113805760 MsG0680035877.01.T01:CDS 30.0%
GATCTTACCTAAAATATTGG+GGG + Chr6:113804486-113804505 MsG0680035877.01.T01:intron 30.0%
GGAAACTTACTGAAAAATGT+TGG + Chr6:113805311-113805330 MsG0680035877.01.T01:intron 30.0%
GGATCTTACCTAAAATATTG+GGG + Chr6:113804485-113804504 MsG0680035877.01.T01:intron 30.0%
TGATTTCAACGAAAATAACG+GGG + Chr6:113805858-113805877 MsG0680035877.01.T01:CDS 30.0%
TGGAATAAAAGATTATAGGG+AGG + Chr6:113805816-113805835 MsG0680035877.01.T01:CDS 30.0%
! CTTTGAGTTCTTGTTCAAAT+TGG - Chr6:113804106-113804125 None:intergenic 30.0%
! GCAAAACTTTATTTGTGAAG+AGG - Chr6:113804246-113804265 None:intergenic 30.0%
! TAAAGTTTTGCATCACTATC+TGG + Chr6:113804255-113804274 MsG0680035877.01.T01:intron 30.0%
!! GATTCGATTGATAATGTATG+TGG - Chr6:113804987-113805006 None:intergenic 30.0%
!! TTGATTTTTGGAAGAGAAGA+TGG + Chr6:113805901-113805920 MsG0680035877.01.T01:CDS 30.0%
!!! GTTTTTGGCTTTTGAAATGA+AGG - Chr6:113805506-113805525 None:intergenic 30.0%
!!! TCTCTAAGATTTTTATACCG+TGG - Chr6:113804861-113804880 None:intergenic 30.0%
!!! TGTCCTTCACATTTTTTTGA+TGG + Chr6:113804779-113804798 MsG0680035877.01.T01:intron 30.0%
AAAAAATTTCTGCCACCAAG+TGG - Chr6:113805551-113805570 None:intergenic 35.0%
AAGAAGAAAAGTTCGAAGCA+TGG + Chr6:113805665-113805684 MsG0680035877.01.T01:CDS 35.0%
AATTGAGAACAACGTCTACA+TGG - Chr6:113804188-113804207 None:intergenic 35.0%
ACAAGAAAAACAGTACCACT+TGG + Chr6:113805533-113805552 MsG0680035877.01.T01:intron 35.0%
AGACATTTAATCTTGGAACG+TGG + Chr6:113805161-113805180 MsG0680035877.01.T01:intron 35.0%
AGAGGATGTTGAATTTGAAG+TGG + Chr6:113805621-113805640 MsG0680035877.01.T01:CDS 35.0%
AGTGAGACTTAATGAAGCAT+CGG + Chr6:113804004-113804023 MsG0680035877.01.T01:five_prime_UTR 35.0%
ATACCGTGGACAAAATATCA+TGG - Chr6:113804847-113804866 None:intergenic 35.0%
ATGAAAGATGTTGAGGATGA+TGG - Chr6:113805462-113805481 None:intergenic 35.0%
CAATGTTAGGCATACATAAG+AGG - Chr6:113804304-113804323 None:intergenic 35.0%
CAGTAGTTCTTCTTCTTCTT+GGG + Chr6:113805789-113805808 MsG0680035877.01.T01:CDS 35.0%
GCGGATCTTACCTAAAATAT+TGG + Chr6:113804483-113804502 MsG0680035877.01.T01:intron 35.0%
TGAAAGATGTTGAGGATGAT+GGG - Chr6:113805461-113805480 None:intergenic 35.0%
TGGTTTAAGGGAAGATGATA+TGG + Chr6:113805594-113805613 MsG0680035877.01.T01:intron 35.0%
! TATTGTCTCTTGTTTCTCTC+CGG - Chr6:113804666-113804685 None:intergenic 35.0%
!! ATTTTTGGAAGAGAAGATGG+TGG + Chr6:113805904-113805923 MsG0680035877.01.T01:CDS 35.0%
!! TTAGAGCTTCAGTTTTGTCT+TGG - Chr6:113804627-113804646 None:intergenic 35.0%
!! TTCAACAAGATGATGGAAAC+TGG + Chr6:113805569-113805588 MsG0680035877.01.T01:intron 35.0%
!!! AGCTATTTTTACAGCCTGTT+CGG + Chr6:113804069-113804088 MsG0680035877.01.T01:five_prime_UTR 35.0%
!!! CCTTCACATTTTTTTGATGG+CGG + Chr6:113804782-113804801 MsG0680035877.01.T01:intron 35.0%
ACCATAGAAAATGGGAAGAC+TGG + Chr6:113805749-113805768 MsG0680035877.01.T01:CDS 40.0%
AGAAAAACAGTACCACTTGG+TGG + Chr6:113805536-113805555 MsG0680035877.01.T01:intron 40.0%
AGAGATACGACAGTATGCAA+TGG + Chr6:113805078-113805097 MsG0680035877.01.T01:intron 40.0%
CATAGTCTCTTGTTCTCTCT+CGG - Chr6:113804697-113804716 None:intergenic 40.0%
CCGCCATCAAAAAAATGTGA+AGG - Chr6:113804785-113804804 None:intergenic 40.0%
GCAGTAGTTCTTCTTCTTCT+TGG + Chr6:113805788-113805807 MsG0680035877.01.T01:CDS 40.0%
GGAAGATGATATGGTTGAAG+AGG + Chr6:113805603-113805622 MsG0680035877.01.T01:exon 40.0%
GGACTTTCATGCTTTCAGAA+TGG - Chr6:113804283-113804302 None:intergenic 40.0%
GGAGAGGAAGTTATTAGAGT+TGG - Chr6:113805440-113805459 None:intergenic 40.0%
GTAGTTTCAGAAATGTGGGT+TGG + Chr6:113805280-113805299 MsG0680035877.01.T01:intron 40.0%
TACCTAAAATATTGGGGGAG+TGG + Chr6:113804491-113804510 MsG0680035877.01.T01:intron 40.0%
! GGTCCATGATATTTTGTCCA+CGG + Chr6:113804841-113804860 MsG0680035877.01.T01:intron 40.0%
! GTTTTGTCTTGGAGTTGTTG+CGG - Chr6:113804616-113804635 None:intergenic 40.0%
! TAAGGGAAGCACAAGAAGAT+AGG + Chr6:113805716-113805735 MsG0680035877.01.T01:CDS 40.0%
!! AAATGTGGGTTGGTTTGGTT+GGG + Chr6:113805290-113805309 MsG0680035877.01.T01:intron 40.0%
!! ATGGAAACTGGCTTGGTTTA+AGG + Chr6:113805581-113805600 MsG0680035877.01.T01:intron 40.0%
!! TGGAAACTGGCTTGGTTTAA+GGG + Chr6:113805582-113805601 MsG0680035877.01.T01:intron 40.0%
!! TTCAGAAATGTGGGTTGGTT+TGG + Chr6:113805285-113805304 MsG0680035877.01.T01:intron 40.0%
!!! AAAATGGGAAGACTGGCTTT+TGG + Chr6:113805756-113805775 MsG0680035877.01.T01:CDS 40.0%
!!! GCTATTTTTACAGCCTGTTC+GGG + Chr6:113804070-113804089 MsG0680035877.01.T01:five_prime_UTR 40.0%
GATTATAGGGAGGAGTTACG+AGG + Chr6:113805826-113805845 MsG0680035877.01.T01:CDS 45.0%
TAGTTGTTTGCCTGCAACAG+CGG + Chr6:113804464-113804483 MsG0680035877.01.T01:intron 45.0%
TCTTCTTCTTCTTGGGAGAG+TGG + Chr6:113805796-113805815 MsG0680035877.01.T01:CDS 45.0%
! GCCAGTCTTCCCATTTTCTA+TGG - Chr6:113805753-113805772 None:intergenic 45.0%
! TGAAGCAACTTTTCCCGAAC+AGG - Chr6:113804086-113804105 None:intergenic 45.0%
! TGCCACTCCCCCAATATTTT+AGG - Chr6:113804496-113804515 None:intergenic 45.0%
!! CAAGATGATGGAAACTGGCT+TGG + Chr6:113805574-113805593 MsG0680035877.01.T01:intron 45.0%
!! GAAATGTGGGTTGGTTTGGT+TGG + Chr6:113805289-113805308 MsG0680035877.01.T01:intron 45.0%
!!! AATATTATAATTTTTTTTTG+AGG + Chr6:113804528-113804547 MsG0680035877.01.T01:intron 5.0%
TAGGTAAGATCCGCTGTTGC+AGG - Chr6:113804477-113804496 None:intergenic 50.0%
!! GATGTTGAGGATGATGGGAG+AGG - Chr6:113805456-113805475 None:intergenic 50.0%
!!! ATGGGAAGACTGGCTTTTGG+AGG + Chr6:113805759-113805778 MsG0680035877.01.T01:CDS 50.0%
ATGCCCGTGACTGCGTTGTT+GGG + Chr6:113805197-113805216 MsG0680035877.01.T01:intron 55.0%
TACCCCCAACAACGCAGTCA+CGG - Chr6:113805204-113805223 None:intergenic 55.0%
ACCCCCAACAACGCAGTCAC+GGG - Chr6:113805203-113805222 None:intergenic 60.0%
CAACAACGCAGTCACGGGCA+TGG - Chr6:113805198-113805217 None:intergenic 60.0%
CATGCCCGTGACTGCGTTGT+TGG + Chr6:113805196-113805215 MsG0680035877.01.T01:intron 60.0%
TAGGGAGGAGTTACGAGGCG+AGG + Chr6:113805831-113805850 MsG0680035877.01.T01:CDS 60.0%
TGCCCGTGACTGCGTTGTTG+GGG + Chr6:113805198-113805217 MsG0680035877.01.T01:intron 60.0%
GCCCGTGACTGCGTTGTTGG+GGG + Chr6:113805199-113805218 MsG0680035877.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 113803933 113805951 113803933 ID=MsG0680035877.01;Name=MsG0680035877.01
Chr6 mRNA 113803933 113805951 113803933 ID=MsG0680035877.01.T01;Parent=MsG0680035877.01;Name=MsG0680035877.01.T01;_AED=0.50;_eAED=0.70;_QI=210|0|0|1|1|1|2|0|112
Chr6 exon 113803933 113804132 113803933 ID=MsG0680035877.01.T01:exon:15331;Parent=MsG0680035877.01.T01
Chr6 exon 113805603 113805951 113805603 ID=MsG0680035877.01.T01:exon:15332;Parent=MsG0680035877.01.T01
Chr6 five_prime_UTR 113803933 113804132 113803933 ID=MsG0680035877.01.T01:five_prime_utr;Parent=MsG0680035877.01.T01
Chr6 five_prime_UTR 113805603 113805612 113805603 ID=MsG0680035877.01.T01:five_prime_utr;Parent=MsG0680035877.01.T01
Chr6 CDS 113805613 113805951 113805613 ID=MsG0680035877.01.T01:cds;Parent=MsG0680035877.01.T01
Gene Sequence

>MsG0680035877.01.T01

ATGGTTGAAGAGGATGTTGAATTTGAAGTGGAAGAAGAACAAGAATTGTCTGAAGAAGAAAAGTTCGAAGCATGGAAGCAAAGAGCAGAAGCTATTATAGATGTAAGGGAAGCACAAGAAGATAGGAAGAATCAAGACCATAGAAAATGGGAAGACTGGCTTTTGGAGGATGACAGCAGTAGTTCTTCTTCTTCTTGGGAGAGTGGAATAAAAGATTATAGGGAGGAGTTACGAGGCGAGGATGATGATTTCAACGAAAATAACGGGGTTGTACAGTCTGTTAAGTATTTGATTTTTGGAAGAGAAGATGGTGGTGATATGATGATGATATGCTTATGA

Protein sequence

>MsG0680035877.01.T01

MVEEDVEFEVEEEQELSEEEKFEAWKQRAEAIIDVREAQEDRKNQDHRKWEDWLLEDDSSSSSSSWESGIKDYREELRGEDDDFNENNGVVQSVKYLIFGREDGGDMMMICL*