AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049135.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049135.01.T01 MTR_1g016270 69.697 198 24 2 1 162 79 276 2.42e-57 181
MsG0080049135.01.T01 MTR_8g097140 46.457 127 52 4 35 157 120 234 8.83e-15 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAAGAAGAAACTAGAATT+TGG 0.282317 contig91end:+3105 MsG0080049135.01.T01:CDS
AAGAAGGAGAAGAGTGTAGA+AGG 0.312453 contig91end:+3219 MsG0080049135.01.T01:CDS
AAGAAGAATGGAAGTGAGTT+AGG 0.374907 contig91end:+3138 MsG0080049135.01.T01:CDS
ATTCTCGGGATGTAGAAATT+AGG 0.415570 contig91end:+3435 MsG0080049135.01.T01:CDS
AGAAGGAGAAGAGTGTAGAA+GGG 0.423276 contig91end:+3220 MsG0080049135.01.T01:CDS
GAAGACACGGAAGAGAGATC+TGG 0.440783 contig91end:+3542 MsG0080049135.01.T01:CDS
GTGGAGGGCTAGTTGATTCT+CGG 0.450550 contig91end:+3420 MsG0080049135.01.T01:CDS
AGTCTAAGAAGAAAATGAAA+AGG 0.460042 contig91end:+3570 MsG0080049135.01.T01:CDS
GGAGAGAGTGTAAAGAAGAA+TGG 0.469789 contig91end:+3126 MsG0080049135.01.T01:CDS
GAGAAGCGTGATAAGAAAGA+TGG 0.475565 contig91end:+3084 MsG0080049135.01.T01:CDS
TGGAGGGCTAGTTGATTCTC+GGG 0.502825 contig91end:+3421 MsG0080049135.01.T01:CDS
AAGACACGGAAGAGAGATCT+GGG 0.505868 contig91end:+3543 MsG0080049135.01.T01:CDS
GAATGGAAGTGAGTTAGGTC+AGG 0.510402 contig91end:+3143 MsG0080049135.01.T01:CDS
GAAGTTGTTGGTTCTGTCGT+GGG 0.512214 contig91end:+3054 MsG0080049135.01.T01:CDS
GAAGGAGAAGAGTGTAGAAG+GGG 0.517307 contig91end:+3221 MsG0080049135.01.T01:CDS
CGAGAAGAAGAACAAGAAGA+AGG 0.521154 contig91end:+3203 MsG0080049135.01.T01:CDS
TGAAGTTGTTGGTTCTGTCG+TGG 0.542751 contig91end:+3053 MsG0080049135.01.T01:CDS
AGACGGAGCAAAGGAAGAAG+AGG 0.546821 contig91end:+3513 MsG0080049135.01.T01:CDS
AAGAGGGAAAGACGGAGCAA+AGG 0.550638 contig91end:+3504 MsG0080049135.01.T01:CDS
AAACCTGGTCGTGAAGTTGT+TGG 0.551304 contig91end:+3042 MsG0080049135.01.T01:CDS
GAGTTAGGTCAGGACGATGA+AGG 0.567638 contig91end:+3153 MsG0080049135.01.T01:CDS
AGATGGTCGAAAGTACTGTT+CGG 0.573130 contig91end:+3010 None:intergenic
GAGTAAATTACATGCTGAAG+AGG 0.574245 contig91end:+3487 MsG0080049135.01.T01:CDS
AATGGTGTGAAGAGTGAAAG+TGG 0.576054 contig91end:+3401 MsG0080049135.01.T01:CDS
GGGACGAGAACAACAGAAAG+AGG 0.576625 contig91end:+3361 MsG0080049135.01.T01:intron
TGTAGAAGGGGAGAATGTGA+AGG 0.579317 contig91end:+3233 MsG0080049135.01.T01:CDS
GACGATGAAGGAAGAACGGA+GGG 0.595325 contig91end:+3165 MsG0080049135.01.T01:CDS
AGTAAATTACATGCTGAAGA+GGG 0.598011 contig91end:+3488 MsG0080049135.01.T01:CDS
GAACCAACAACTTCACGACC+AGG 0.602335 contig91end:-3045 MsG0080049135.01.T01:intergenic
GTTCGGAGTGATGTTAAACC+TGG 0.607851 contig91end:+3027 MsG0080049135.01.T01:CDS
GTTGTTGGTTCTGTCGTGGG+TGG 0.608189 contig91end:+3057 MsG0080049135.01.T01:CDS
ACGAGAACAACAGAAAGAGG+TGG 0.612657 contig91end:+3364 MsG0080049135.01.T01:CDS
TCAGGACGATGAAGGAAGAA+CGG 0.621358 contig91end:+3161 MsG0080049135.01.T01:CDS
ACATGCTGAAGAGGGAAAGA+CGG 0.635975 contig91end:+3496 MsG0080049135.01.T01:CDS
GAAGAGGAAGAATGAAGACA+CGG 0.643138 contig91end:+3529 MsG0080049135.01.T01:CDS
GTGTGAAGAGTGAAAGTGGA+GGG 0.650346 contig91end:+3405 MsG0080049135.01.T01:CDS
GGACGATGAAGGAAGAACGG+AGG 0.651421 contig91end:+3164 MsG0080049135.01.T01:CDS
GAAAATGAAAAGGAAACACG+AGG 0.691720 contig91end:+3580 MsG0080049135.01.T01:CDS
AAAATGAAAAGGAAACACGA+GGG 0.733622 contig91end:+3581 MsG0080049135.01.T01:CDS
AGACACGGAAGAGAGATCTG+GGG 0.742079 contig91end:+3544 MsG0080049135.01.T01:CDS
GGTGTGAAGAGTGAAAGTGG+AGG 0.804724 contig91end:+3404 MsG0080049135.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTCTAAGAAGAAAATGAAA+AGG + contig91end:3570-3589 MsG0080049135.01.T01:CDS 25.0%
AAAATGAAAAGGAAACACGA+GGG + contig91end:3581-3600 MsG0080049135.01.T01:CDS 30.0%
AATGTGAAGGTTGAAAGTAA+AGG + contig91end:3246-3265 MsG0080049135.01.T01:intron 30.0%
AGTAAATTACATGCTGAAGA+GGG + contig91end:3488-3507 MsG0080049135.01.T01:CDS 30.0%
ATGTGAAGGTTGAAAGTAAA+GGG + contig91end:3247-3266 MsG0080049135.01.T01:intron 30.0%
GGAAAGAAGAAACTAGAATT+TGG + contig91end:3105-3124 MsG0080049135.01.T01:CDS 30.0%
TCGAAAAAGAGAAATGAAGT+GGG + contig91end:3300-3319 MsG0080049135.01.T01:intron 30.0%
AAGAAGAATGGAAGTGAGTT+AGG + contig91end:3138-3157 MsG0080049135.01.T01:CDS 35.0%
AAGGAGAAGAATTTAGAGGT+TGG + contig91end:3329-3348 MsG0080049135.01.T01:intron 35.0%
ATTCTCGGGATGTAGAAATT+AGG + contig91end:3435-3454 MsG0080049135.01.T01:CDS 35.0%
CGAAAAAGAGAAATGAAGTG+GGG + contig91end:3301-3320 MsG0080049135.01.T01:intron 35.0%
GAAAATGAAAAGGAAACACG+AGG + contig91end:3580-3599 MsG0080049135.01.T01:CDS 35.0%
GAGTAAATTACATGCTGAAG+AGG + contig91end:3487-3506 MsG0080049135.01.T01:CDS 35.0%
GTCGAAAAAGAGAAATGAAG+TGG + contig91end:3299-3318 MsG0080049135.01.T01:intron 35.0%
GTGAAAGGAGAAGAATTTAG+AGG + contig91end:3325-3344 MsG0080049135.01.T01:intron 35.0%
GTGGAGAAAAAACTAAGCAA+TGG + contig91end:3383-3402 MsG0080049135.01.T01:CDS 35.0%
TTAGAGGTTGGAAATGTGAA+GGG + contig91end:3341-3360 MsG0080049135.01.T01:intron 35.0%
TTTAGAGGTTGGAAATGTGA+AGG + contig91end:3340-3359 MsG0080049135.01.T01:intron 35.0%
! CGAAAAAGAAGCATGAAACT+GGG + contig91end:3459-3478 MsG0080049135.01.T01:CDS 35.0%
! TCGAAAAAGAAGCATGAAAC+TGG + contig91end:3458-3477 MsG0080049135.01.T01:CDS 35.0%
AATGGTGTGAAGAGTGAAAG+TGG + contig91end:3401-3420 MsG0080049135.01.T01:CDS 40.0%
CGAGAAGAAGAACAAGAAGA+AGG + contig91end:3203-3222 MsG0080049135.01.T01:CDS 40.0%
CTAATTTCTAACTCCTGAGG+AGG - contig91end:3279-3298 MsG0080049135.01.T01:intergenic 40.0%
CTCCTCAGGAGTTAGAAATT+AGG + contig91end:3277-3296 MsG0080049135.01.T01:intron 40.0%
GAAGAGGAAGAATGAAGACA+CGG + contig91end:3529-3548 MsG0080049135.01.T01:CDS 40.0%
GACCTAATTTCTAACTCCTG+AGG - contig91end:3282-3301 MsG0080049135.01.T01:intergenic 40.0%
! AAGAAGGAGAAGAGTGTAGA+AGG + contig91end:3219-3238 MsG0080049135.01.T01:CDS 40.0%
! AGAAGGAGAAGAGTGTAGAA+GGG + contig91end:3220-3239 MsG0080049135.01.T01:CDS 40.0%
! GAGAAGCGTGATAAGAAAGA+TGG + contig91end:3084-3103 MsG0080049135.01.T01:CDS 40.0%
! GGAGAGAGTGTAAAGAAGAA+TGG + contig91end:3126-3145 MsG0080049135.01.T01:CDS 40.0%
AAACCTGGTCGTGAAGTTGT+TGG + contig91end:3042-3061 MsG0080049135.01.T01:CDS 45.0%
AAGACACGGAAGAGAGATCT+GGG + contig91end:3543-3562 MsG0080049135.01.T01:CDS 45.0%
ACATGCTGAAGAGGGAAAGA+CGG + contig91end:3496-3515 MsG0080049135.01.T01:CDS 45.0%
ACGAGAACAACAGAAAGAGG+TGG + contig91end:3364-3383 MsG0080049135.01.T01:CDS 45.0%
GAAATGAAGTGGGGAGTGAA+AGG + contig91end:3310-3329 MsG0080049135.01.T01:intron 45.0%
GAATGGAAGTGAGTTAGGTC+AGG + contig91end:3143-3162 MsG0080049135.01.T01:CDS 45.0%
GTGTGAAGAGTGAAAGTGGA+GGG + contig91end:3405-3424 MsG0080049135.01.T01:CDS 45.0%
GTTCGGAGTGATGTTAAACC+TGG + contig91end:3027-3046 MsG0080049135.01.T01:CDS 45.0%
TAAAGGGATAGTTCCTCCTC+AGG + contig91end:3263-3282 MsG0080049135.01.T01:intron 45.0%
TCAGGACGATGAAGGAAGAA+CGG + contig91end:3161-3180 MsG0080049135.01.T01:CDS 45.0%
TGTAGAAGGGGAGAATGTGA+AGG + contig91end:3233-3252 MsG0080049135.01.T01:CDS 45.0%
! GAAGGAGAAGAGTGTAGAAG+GGG + contig91end:3221-3240 MsG0080049135.01.T01:CDS 45.0%
!! GAAGTTGTTGGTTCTGTCGT+GGG + contig91end:3054-3073 MsG0080049135.01.T01:CDS 45.0%
!! TGAAGTTGTTGGTTCTGTCG+TGG + contig91end:3053-3072 MsG0080049135.01.T01:CDS 45.0%
AAGAGGGAAAGACGGAGCAA+AGG + contig91end:3504-3523 MsG0080049135.01.T01:CDS 50.0%
AGACACGGAAGAGAGATCTG+GGG + contig91end:3544-3563 MsG0080049135.01.T01:CDS 50.0%
AGACGGAGCAAAGGAAGAAG+AGG + contig91end:3513-3532 MsG0080049135.01.T01:CDS 50.0%
GAACCAACAACTTCACGACC+AGG - contig91end:3048-3067 MsG0080049135.01.T01:intergenic 50.0%
GAAGACACGGAAGAGAGATC+TGG + contig91end:3542-3561 MsG0080049135.01.T01:CDS 50.0%
GACGATGAAGGAAGAACGGA+GGG + contig91end:3165-3184 MsG0080049135.01.T01:CDS 50.0%
GAGTTAGGTCAGGACGATGA+AGG + contig91end:3153-3172 MsG0080049135.01.T01:CDS 50.0%
GGGACGAGAACAACAGAAAG+AGG + contig91end:3361-3380 MsG0080049135.01.T01:intron 50.0%
GGTGTGAAGAGTGAAAGTGG+AGG + contig91end:3404-3423 MsG0080049135.01.T01:CDS 50.0%
!! GTGGAGGGCTAGTTGATTCT+CGG + contig91end:3420-3439 MsG0080049135.01.T01:CDS 50.0%
!! TGGAGGGCTAGTTGATTCTC+GGG + contig91end:3421-3440 MsG0080049135.01.T01:CDS 50.0%
GGACGATGAAGGAAGAACGG+AGG + contig91end:3164-3183 MsG0080049135.01.T01:CDS 55.0%
!! GTTGTTGGTTCTGTCGTGGG+TGG + contig91end:3057-3076 MsG0080049135.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
contig91end gene 3012 3607 3012 ID=MsG0080049135.01;Name=MsG0080049135.01
contig91end mRNA 3012 3607 3012 ID=MsG0080049135.01.T01;Parent=MsG0080049135.01;Name=MsG0080049135.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|162
contig91end exon 3012 3254 3012 ID=MsG0080049135.01.T01:exon:9156;Parent=MsG0080049135.01.T01
contig91end exon 3362 3607 3362 ID=MsG0080049135.01.T01:exon:9157;Parent=MsG0080049135.01.T01
contig91end CDS 3012 3254 3012 ID=MsG0080049135.01.T01:cds;Parent=MsG0080049135.01.T01
contig91end CDS 3362 3607 3362 ID=MsG0080049135.01.T01:cds;Parent=MsG0080049135.01.T01
Gene Sequence

>MsG0080049135.01.T01

ATGGTCGAAAGTACTGTTCGGAGTGATGTTAAACCTGGTCGTGAAGTTGTTGGTTCTGTCGTGGGTGGAAGTGAGAAGCGTGATAAGAAAGATGGAAAGAAGAAACTAGAATTTGGAGAGAGTGTAAAGAAGAATGGAAGTGAGTTAGGTCAGGACGATGAAGGAAGAACGGAGGGAAAAAAGCAAAAGAGCGAGAAGAAGAACAAGAAGAAGGAGAAGAGTGTAGAAGGGGAGAATGTGAAGGGACGAGAACAACAGAAAGAGGTGGAGAAAAAACTAAGCAATGGTGTGAAGAGTGAAAGTGGAGGGCTAGTTGATTCTCGGGATGTAGAAATTAGGTCGAAAAAGAAGCATGAAACTGGGAGTGAGAGTAAATTACATGCTGAAGAGGGAAAGACGGAGCAAAGGAAGAAGAGGAAGAATGAAGACACGGAAGAGAGATCTGGGGAGAAGTCTAAGAAGAAAATGAAAAGGAAACACGAGGGTTAA

Protein sequence

>MsG0080049135.01.T01

MVESTVRSDVKPGREVVGSVVGGSEKRDKKDGKKKLEFGESVKKNGSELGQDDEGRTEGKKQKSEKKNKKKEKSVEGENVKGREQQKEVEKKLSNGVKSESGGLVDSRDVEIRSKKKHETGSESKLHAEEGKTEQRKKRKNEDTEERSGEKSKKKMKRKHEG*