AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010013.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010013.01.T01 MTR_2g076300 77.922 77 11 1 1 77 1 71 5.13e-36 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010013.01.T01 AT1G10865 67.692 65 15 1 1 65 1 59 1.33e-23 84.7
MsG0280010013.01.T01 AT1G10865 65.672 67 17 1 1 67 1 61 1.64e-23 85.1

Find 13 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTGAGTGTGTTGGTCTT+AGG 0.172998 2:+62837438 MsG0280010013.01.T01:CDS
AGCTGAGTGTGTTGGTCTTA+GGG 0.357346 2:+62837439 MsG0280010013.01.T01:CDS
GGCTAATCCTTTGCAAGACT+TGG 0.367609 2:-62837233 None:intergenic
ACCTATTTCAATTGCAAAAG+AGG 0.400990 2:+62837464 MsG0280010013.01.T01:CDS
CAGTGAATCTGATTGTGTTA+AGG 0.440858 2:+62837276 MsG0280010013.01.T01:CDS
TCAATTTCAGCTGAGTGTGT+TGG 0.497821 2:+62837431 MsG0280010013.01.T01:CDS
ACACACTCAGCTGAAATTGA+AGG 0.502802 2:-62837428 None:intergenic
TTTCAATTGCAAAAGAGGAC+AGG 0.525074 2:+62837469 MsG0280010013.01.T01:CDS
AGATCATACAAGGAGTGTGC+TGG 0.532397 2:+62837395 MsG0280010013.01.T01:CDS
GAGACACTTAACTAATTCCA+TGG 0.532404 2:-62837254 None:intergenic
GTCTTGCAAAGGATTAGCCA+TGG 0.600408 2:+62837237 MsG0280010013.01.T01:CDS
GGTTGATAATAGATCATACA+AGG 0.601750 2:+62837385 MsG0280010013.01.T01:intron
ACAATGTCCAAGTCTTGCAA+AGG 0.613550 2:+62837226 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTTTATGATTATGAAT+AGG + Chr2:62837364-62837383 MsG0280010013.01.T01:intron 15.0%
!!! GATTTTTGTGATGTTAAAAT+GGG + Chr2:62837341-62837360 MsG0280010013.01.T01:intron 20.0%
!!! TGATTTTTGTGATGTTAAAA+TGG + Chr2:62837340-62837359 MsG0280010013.01.T01:intron 20.0%
!!! GGCAAGTTTTTATTATTTCT+TGG + Chr2:62837490-62837509 MsG0280010013.01.T01:CDS 25.0%
ACCTATTTCAATTGCAAAAG+AGG + Chr2:62837464-62837483 MsG0280010013.01.T01:CDS 30.0%
! TCCTCTTTTGCAATTGAAAT+AGG - Chr2:62837468-62837487 None:intergenic 30.0%
!! GGTTGATAATAGATCATACA+AGG + Chr2:62837385-62837404 MsG0280010013.01.T01:intron 30.0%
CAGTGAATCTGATTGTGTTA+AGG + Chr2:62837276-62837295 MsG0280010013.01.T01:CDS 35.0%
GAGACACTTAACTAATTCCA+TGG - Chr2:62837257-62837276 None:intergenic 35.0%
TTTCAATTGCAAAAGAGGAC+AGG + Chr2:62837469-62837488 MsG0280010013.01.T01:CDS 35.0%
ACACACTCAGCTGAAATTGA+AGG - Chr2:62837431-62837450 None:intergenic 40.0%
TCAATTTCAGCTGAGTGTGT+TGG + Chr2:62837431-62837450 MsG0280010013.01.T01:CDS 40.0%
GGCTAATCCTTTGCAAGACT+TGG - Chr2:62837236-62837255 None:intergenic 45.0%
! AGATCATACAAGGAGTGTGC+TGG + Chr2:62837395-62837414 MsG0280010013.01.T01:CDS 45.0%
!! AGCTGAGTGTGTTGGTCTTA+GGG + Chr2:62837439-62837458 MsG0280010013.01.T01:CDS 45.0%
!! GTCTTGCAAAGGATTAGCCA+TGG + Chr2:62837237-62837256 MsG0280010013.01.T01:CDS 45.0%
!! CAGCTGAGTGTGTTGGTCTT+AGG + Chr2:62837438-62837457 MsG0280010013.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 62837229 62837535 62837229 ID=MsG0280010013.01;Name=MsG0280010013.01
Chr2 mRNA 62837229 62837535 62837229 ID=MsG0280010013.01.T01;Parent=MsG0280010013.01;Name=MsG0280010013.01.T01;_AED=0.50;_eAED=0.61;_QI=0|0|0|1|0|0|2|0|78
Chr2 exon 62837229 62837315 62837229 ID=MsG0280010013.01.T01:exon:4895;Parent=MsG0280010013.01.T01
Chr2 exon 62837386 62837535 62837386 ID=MsG0280010013.01.T01:exon:4896;Parent=MsG0280010013.01.T01
Chr2 CDS 62837229 62837315 62837229 ID=MsG0280010013.01.T01:cds;Parent=MsG0280010013.01.T01
Chr2 CDS 62837386 62837535 62837386 ID=MsG0280010013.01.T01:cds;Parent=MsG0280010013.01.T01
Gene Sequence

>MsG0280010013.01.T01

ATGTCCAAGTCTTGCAAAGGATTAGCCATGGAATTAGTTAAGTGTCTCAGTGAATCTGATTGTGTTAAGGTTCGTTTTTTTATTAGTGTTGATAATAGATCATACAAGGAGTGTGCTGGAGAGAAGACTCCTTCAATTTCAGCTGAGTGTGTTGGTCTTAGGGAAACCTATTTCAATTGCAAAAGAGGACAGGCAAGTTTTTATTATTTCTTGGATTTATCATGTTACATTTGTTAG

Protein sequence

>MsG0280010013.01.T01

MSKSCKGLAMELVKCLSESDCVKVRFFISVDNRSYKECAGEKTPSISAECVGLRETYFNCKRGQASFYYFLDLSCYIC*