AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380018033.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380018033.01.T01 MTR_3g117570 95.652 92 3 1 1 92 1 91 1.74e-58 174
MsG0380018033.01.T01 MTR_3g117570 95.062 81 3 1 1 81 1 80 3.72e-49 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380018033.01.T01 AT2G21290 62.651 83 29 1 10 92 18 98 1.25e-20 79.0

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGATCATTTGTTCTCTCTT+CGG 0.404252 3:+103558523 None:intergenic
AAAAGATTCAAGGGCTCGTA+TGG 0.464506 3:-103558556 MsG0380018033.01.T01:CDS
AAAGAAAGGGAAAAGATTCA+AGG 0.481880 3:-103558566 MsG0380018033.01.T01:CDS
TGAAATCAAATGAGCTTGAA+AGG 0.487197 3:+103558451 None:intergenic
AAGTTCCCAGGTCTACTCCT+TGG 0.493148 3:-103558480 MsG0380018033.01.T01:CDS
ACGCCTTGTGCTTCTTCTGC+CGG 0.494602 3:-103558634 MsG0380018033.01.T01:CDS
GCTGCACGGTGGTTGATAAG+AGG 0.503001 3:-103558670 MsG0380018033.01.T01:CDS
GATCGAACGAATCAAAGATA+AGG 0.533385 3:-103558506 MsG0380018033.01.T01:CDS
CAAATGAGCTTGAAAGGAAG+AGG 0.534374 3:+103558457 None:intergenic
GCTCCGATAGTGTGCGGACG+CGG 0.535798 3:-103558601 MsG0380018033.01.T01:CDS
TCACCGCGTCCGCACACTAT+CGG 0.538358 3:+103558598 None:intergenic
AGCTTGAAAGGAAGAGGCCA+AGG 0.539805 3:+103558463 None:intergenic
TATCAACCACCGTGCAGCTA+CGG 0.541002 3:+103558675 None:intergenic
AAGATAAGGTTGAAGTTCCC+AGG 0.546325 3:-103558492 MsG0380018033.01.T01:CDS
AAGAGGCCAAGGAGTAGACC+TGG 0.562320 3:+103558474 None:intergenic
TATCGGAGCAGCTGTTACGC+CGG 0.576176 3:+103558615 None:intergenic
AAGAAAGGGAAAAGATTCAA+GGG 0.576778 3:-103558565 MsG0380018033.01.T01:CDS
AGCGGTGCACCGTAGCTGCA+CGG 0.586905 3:-103558684 MsG0380018033.01.T01:CDS
GTTGATAAGAGGAGCGTCGT+CGG 0.589779 3:-103558659 MsG0380018033.01.T01:CDS
AGAGGCCAAGGAGTAGACCT+GGG 0.623067 3:+103558475 None:intergenic
AAAGATTCAAGGGCTCGTAT+GGG 0.635720 3:-103558555 MsG0380018033.01.T01:CDS
GTGGCGCTAGTGTGATGCAG+CGG 0.656151 3:-103558702 MsG0380018033.01.T01:CDS
AAAGTGTGTATGGCGAGTGG+TGG 0.695087 3:-103558721 None:intergenic
ACAGCTGCTCCGATAGTGTG+CGG 0.696198 3:-103558607 MsG0380018033.01.T01:CDS
AGAAAAGTGTGTATGGCGAG+TGG 0.701894 3:-103558724 None:intergenic
ACGCCGGCAGAAGAAGCACA+AGG 0.711011 3:+103558631 None:intergenic
GGTGCACCGTAGCTGCACGG+TGG 0.772757 3:-103558681 MsG0380018033.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTGATAAAAAGACAAAGAAA+GGG - Chr3:103558589-103558608 MsG0380018033.01.T01:CDS 25.0%
AAAGAAAGGGAAAAGATTCA+AGG - Chr3:103558602-103558621 MsG0380018033.01.T01:CDS 30.0%
AAGAAAGGGAAAAGATTCAA+GGG - Chr3:103558603-103558622 MsG0380018033.01.T01:CDS 30.0%
GGTGATAAAAAGACAAAGAA+AGG - Chr3:103558588-103558607 MsG0380018033.01.T01:CDS 30.0%
GATCGAACGAATCAAAGATA+AGG - Chr3:103558662-103558681 MsG0380018033.01.T01:CDS 35.0%
TCGATCATTTGTTCTCTCTT+CGG + Chr3:103558648-103558667 None:intergenic 35.0%
AAAAGATTCAAGGGCTCGTA+TGG - Chr3:103558612-103558631 MsG0380018033.01.T01:CDS 40.0%
AAAGATTCAAGGGCTCGTAT+GGG - Chr3:103558613-103558632 MsG0380018033.01.T01:CDS 40.0%
AAGATAAGGTTGAAGTTCCC+AGG - Chr3:103558676-103558695 MsG0380018033.01.T01:CDS 40.0%
CAAATGAGCTTGAAAGGAAG+AGG + Chr3:103558714-103558733 None:intergenic 40.0%
AAGTTCCCAGGTCTACTCCT+TGG - Chr3:103558688-103558707 MsG0380018033.01.T01:CDS 50.0%
AGCTTGAAAGGAAGAGGCCA+AGG + Chr3:103558708-103558727 None:intergenic 50.0%
TATCAACCACCGTGCAGCTA+CGG + Chr3:103558496-103558515 None:intergenic 50.0%
!! GTTGATAAGAGGAGCGTCGT+CGG - Chr3:103558509-103558528 MsG0380018033.01.T01:CDS 50.0%
AAGAGGCCAAGGAGTAGACC+TGG + Chr3:103558697-103558716 None:intergenic 55.0%
ACAGCTGCTCCGATAGTGTG+CGG - Chr3:103558561-103558580 MsG0380018033.01.T01:CDS 55.0%
ACGCCTTGTGCTTCTTCTGC+CGG - Chr3:103558534-103558553 MsG0380018033.01.T01:CDS 55.0%
AGAGGCCAAGGAGTAGACCT+GGG + Chr3:103558696-103558715 None:intergenic 55.0%
TATCGGAGCAGCTGTTACGC+CGG + Chr3:103558556-103558575 None:intergenic 55.0%
!! GCTGCACGGTGGTTGATAAG+AGG - Chr3:103558498-103558517 MsG0380018033.01.T01:CDS 55.0%
GTGGCGCTAGTGTGATGCAG+CGG - Chr3:103558466-103558485 MsG0380018033.01.T01:CDS 60.0%
TCACCGCGTCCGCACACTAT+CGG + Chr3:103558573-103558592 None:intergenic 60.0%
! ACGCCGGCAGAAGAAGCACA+AGG + Chr3:103558540-103558559 None:intergenic 60.0%
AGCGGTGCACCGTAGCTGCA+CGG - Chr3:103558484-103558503 MsG0380018033.01.T01:CDS 65.0%
GCTCCGATAGTGTGCGGACG+CGG - Chr3:103558567-103558586 MsG0380018033.01.T01:CDS 65.0%
GGTGCACCGTAGCTGCACGG+TGG - Chr3:103558487-103558506 MsG0380018033.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 103558456 103558734 103558456 ID=MsG0380018033.01;Name=MsG0380018033.01
Chr3 mRNA 103558456 103558734 103558456 ID=MsG0380018033.01.T01;Parent=MsG0380018033.01;Name=MsG0380018033.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|92
Chr3 exon 103558456 103558734 103558456 ID=MsG0380018033.01.T01:exon:20268;Parent=MsG0380018033.01.T01
Chr3 CDS 103558456 103558734 103558456 ID=MsG0380018033.01.T01:cds;Parent=MsG0380018033.01.T01
Gene Sequence

>MsG0380018033.01.T01

ATGGCGAGTGGTGGCGCTAGTGTGATGCAGCGGTGCACCGTAGCTGCACGGTGGTTGATAAGAGGAGCGTCGTCGGAGACGCCTTGTGCTTCTTCTGCCGGCGTAACAGCTGCTCCGATAGTGTGCGGACGCGGTGATAAAAAGACAAAGAAAGGGAAAAGATTCAAGGGCTCGTATGGGAAATCTCGTCCGAAGAGAGAACAAATGATCGAACGAATCAAAGATAAGGTTGAAGTTCCCAGGTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATTTGA

Protein sequence

>MsG0380018033.01.T01

MASGGASVMQRCTVAARWLIRGASSETPCASSAGVTAAPIVCGRGDKKTKKGKRFKGSYGKSRPKREQMIERIKDKVEVPRSTPWPLPFKLI*