AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035765.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035765.01.T01 MTR_6g088605 96.721 122 4 0 1 122 1 122 5.53e-85 246
MsG0680035765.01.T01 MTR_7g103190 90.164 122 12 0 1 122 1 122 7.77e-80 233
MsG0680035765.01.T01 MTR_5g037160 92.308 39 3 0 62 100 409 447 4.11e-17 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035765.01.T01 AT3G18760 62.687 134 49 1 1 134 1 133 8.23e-56 171
MsG0680035765.01.T01 AT3G18760 59.701 134 48 2 1 134 1 128 9.36e-50 156

Find 30 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGCTCTTTGTTGATTTC+GGG 0.181534 6:+111933261 None:intergenic
ATGCAGCTCTTTGTTGATTT+CGG 0.208605 6:+111933260 None:intergenic
GTCACTGAGGTTAAGAGTTT+TGG 0.239764 6:-111935025 MsG0680035765.01.T01:CDS
TCATGCTCACTTGCATCATT+TGG 0.288871 6:+111933292 None:intergenic
CTCTAATTGGTTTAGTTTCA+AGG 0.335670 6:-111935081 MsG0680035765.01.T01:CDS
TTGGTTTAGTTTCAAGGATT+GGG 0.366475 6:-111935075 MsG0680035765.01.T01:CDS
TATGATAACCTTGGCGCAAC+TGG 0.376873 6:-111933066 MsG0680035765.01.T01:CDS
AACATGTGTGTAAAAGAAAT+GGG 0.380053 6:-111935051 MsG0680035765.01.T01:CDS
ATTGGTTTAGTTTCAAGGAT+TGG 0.385789 6:-111935076 MsG0680035765.01.T01:CDS
GCGTGATGGCAGATTCTATC+AGG 0.387307 6:-111934972 MsG0680035765.01.T01:intron
GTAGTTTGTCCTCCTTGTTT+AGG 0.396913 6:+111933235 None:intergenic
GGAACGATGCAGTTGGGTTA+TGG 0.403450 6:-111935004 MsG0680035765.01.T01:CDS
AAACATGTGTGTAAAAGAAA+TGG 0.446983 6:-111935052 MsG0680035765.01.T01:CDS
CAAACTACTGCGTTGGCTAC+TGG 0.454974 6:-111933220 MsG0680035765.01.T01:CDS
AACAAAAGCATGCAATCATA+AGG 0.460708 6:+111935127 None:intergenic
GTATCACTATCCTCGCCATC+AGG 0.482219 6:+111933111 None:intergenic
CTTGAGTTCATGGGAGATGA+AGG 0.503780 6:-111933171 MsG0680035765.01.T01:CDS
AAAATTGGGCTTGAGTTCAT+GGG 0.544552 6:-111933180 MsG0680035765.01.T01:CDS
TCTGAAATAAATAGACCTGA+TGG 0.565799 6:-111933126 MsG0680035765.01.T01:CDS
AGGAGGACAAACTACTGCGT+TGG 0.582013 6:-111933227 MsG0680035765.01.T01:CDS
CAACTTTACCAGTTGCGCCA+AGG 0.585916 6:+111933058 None:intergenic
GATGATGAATATGATAACCT+TGG 0.610678 6:-111933075 MsG0680035765.01.T01:CDS
AATAAATAGACCTGATGGCG+AGG 0.631134 6:-111933121 MsG0680035765.01.T01:CDS
AAGAAATGGGGTTGTCACTG+AGG 0.631658 6:-111935038 MsG0680035765.01.T01:CDS
GGCGAGGATAGTGATACAGA+CGG 0.636843 6:-111933105 MsG0680035765.01.T01:CDS
AAAAGCATGCAATCATAAGG+TGG 0.660043 6:+111935130 None:intergenic
AATGATGCAAGTGAGCATGA+TGG 0.687340 6:-111933289 MsG0680035765.01.T01:CDS
ACATGTGTGTAAAAGAAATG+GGG 0.688446 6:-111935050 MsG0680035765.01.T01:CDS
AGAGCTGCATTACCTAAACA+AGG 0.691182 6:-111933247 MsG0680035765.01.T01:CDS
GCTGCATTACCTAAACAAGG+AGG 0.778109 6:-111933244 MsG0680035765.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATAGATTATTTGTATT+TGG - Chr6:111933861-111933880 MsG0680035765.01.T01:intron 10.0%
!! AATAAATAAATAAATCATGC+AGG + Chr6:111933437-111933456 None:intergenic 15.0%
!! TATAAAAATAGAATTTGACA+TGG - Chr6:111933932-111933951 MsG0680035765.01.T01:intron 15.0%
!!! TCTATTTTTTAATGAATGTA+GGG - Chr6:111934606-111934625 MsG0680035765.01.T01:intron 15.0%
!!! TGTTTTTATAAACTATCTTA+AGG - Chr6:111934008-111934027 MsG0680035765.01.T01:intron 15.0%
!!! TTCTATTTTTTAATGAATGT+AGG - Chr6:111934605-111934624 MsG0680035765.01.T01:intron 15.0%
!! ATAGTTTATAAAAACAGTGT+AGG + Chr6:111934004-111934023 None:intergenic 20.0%
!! CAAACTTTGATAAATTATTG+AGG - Chr6:111933333-111933352 MsG0680035765.01.T01:intron 20.0%
!! CTATTTAACAATACTGAAAA+TGG + Chr6:111934160-111934179 None:intergenic 20.0%
!! TAGAGAATTTCAATCAAATA+CGG + Chr6:111933635-111933654 None:intergenic 20.0%
!!! AGTCTTTTAGATTTATAACA+TGG + Chr6:111934100-111934119 None:intergenic 20.0%
! AAACATGTGTGTAAAAGAAA+TGG - Chr6:111933140-111933159 MsG0680035765.01.T01:CDS 25.0%
! AAATAGAATTTGACATGGAT+AGG - Chr6:111933937-111933956 MsG0680035765.01.T01:intron 25.0%
! AACATGTGTGTAAAAGAAAT+GGG - Chr6:111933141-111933160 MsG0680035765.01.T01:CDS 25.0%
! GAATTTGAACAAATTGGTAT+TGG - Chr6:111934197-111934216 MsG0680035765.01.T01:intron 25.0%
! GTATTGGAATTTGAACAAAT+TGG - Chr6:111934191-111934210 MsG0680035765.01.T01:intron 25.0%
! TAAGGATGAAATCAATTCAT+GGG - Chr6:111934026-111934045 MsG0680035765.01.T01:intron 25.0%
! TTAAGGATGAAATCAATTCA+TGG - Chr6:111934025-111934044 MsG0680035765.01.T01:intron 25.0%
! TTATTGGATGTTTAAATAGC+AGG - Chr6:111933803-111933822 MsG0680035765.01.T01:intron 25.0%
! TTGTACTAAATATCGTATCA+CGG + Chr6:111934246-111934265 None:intergenic 25.0%
! TTGTATTTGGATATATGCAT+TGG - Chr6:111933874-111933893 MsG0680035765.01.T01:intron 25.0%
!! AATTTTGCAAAACATGTATC+CGG + Chr6:111933272-111933291 None:intergenic 25.0%
!! TGTTTTGCAAAATTGACTAT+TGG - Chr6:111933278-111933297 MsG0680035765.01.T01:CDS 25.0%
!!! ATAGACAGGGTTTTAAAAAA+TGG - Chr6:111933527-111933546 MsG0680035765.01.T01:intron 25.0%
!!! CTTTTTAATTAAAGAGAGAC+AGG - Chr6:111933391-111933410 MsG0680035765.01.T01:intron 25.0%
!!! TGACTTTTAGAACTTTCAAT+AGG + Chr6:111934411-111934430 None:intergenic 25.0%
AAAATGGTAATATCCACATG+GGG + Chr6:111934144-111934163 None:intergenic 30.0%
AAATAAATCATGCAGGCATT+GGG + Chr6:111933430-111933449 None:intergenic 30.0%
ACATGTGTGTAAAAGAAATG+GGG - Chr6:111933142-111933161 MsG0680035765.01.T01:CDS 30.0%
AGTGTTGTAATCATAGATTG+TGG + Chr6:111934342-111934361 None:intergenic 30.0%
ATACTTGTTTAGGTAGTGTT+TGG + Chr6:111934072-111934091 None:intergenic 30.0%
ATATAATCTAGGTGATGTGA+TGG - Chr6:111933464-111933483 MsG0680035765.01.T01:intron 30.0%
CAACAACATAATTGTGATTG+AGG - Chr6:111933600-111933619 MsG0680035765.01.T01:intron 30.0%
CATGGCATAAATACTTGTTT+AGG + Chr6:111934082-111934101 None:intergenic 30.0%
CTATATGCATCACACTAATT+TGG + Chr6:111933511-111933530 None:intergenic 30.0%
GAAAATGGTAATATCCACAT+GGG + Chr6:111934145-111934164 None:intergenic 30.0%
GATGATGAATATGATAACCT+TGG - Chr6:111935117-111935136 MsG0680035765.01.T01:CDS 30.0%
GATGTTTAAATAGCAGGTTA+TGG - Chr6:111933809-111933828 MsG0680035765.01.T01:intron 30.0%
TAAATAAATCATGCAGGCAT+TGG + Chr6:111933431-111933450 None:intergenic 30.0%
TCATAGATTGTGGAAAATAG+CGG + Chr6:111934332-111934351 None:intergenic 30.0%
TGAAAATGGTAATATCCACA+TGG + Chr6:111934146-111934165 None:intergenic 30.0%
TGAAATTCTCTACAACATCA+AGG - Chr6:111933643-111933662 MsG0680035765.01.T01:intron 30.0%
TTGTTAAATAGCAGCTATAG+TGG - Chr6:111934169-111934188 MsG0680035765.01.T01:intron 30.0%
TTTAATTAAAGAGAGACAGG+AGG - Chr6:111933394-111933413 MsG0680035765.01.T01:intron 30.0%
! AACAAAAGCATGCAATCATA+AGG + Chr6:111933068-111933087 None:intergenic 30.0%
! ATTGGTTTAGTTTCAAGGAT+TGG - Chr6:111933116-111933135 MsG0680035765.01.T01:CDS 30.0%
! CTCTAATTGGTTTAGTTTCA+AGG - Chr6:111933111-111933130 MsG0680035765.01.T01:CDS 30.0%
! GTGAAAAAAGAAGCTCTAAT+TGG - Chr6:111933098-111933117 MsG0680035765.01.T01:CDS 30.0%
! TCTGAAATAAATAGACCTGA+TGG - Chr6:111935066-111935085 MsG0680035765.01.T01:CDS 30.0%
! TTGGTTTAGTTTCAAGGATT+GGG - Chr6:111933117-111933136 MsG0680035765.01.T01:CDS 30.0%
!!! AGAGCTTCTTTTTTCACATT+CGG + Chr6:111933095-111933114 None:intergenic 30.0%
!!! CTATGCAGAGTTTTAAACAA+CGG + Chr6:111933699-111933718 None:intergenic 30.0%
AAAAATTGGGCTTGAGTTCA+TGG - Chr6:111935011-111935030 MsG0680035765.01.T01:CDS 35.0%
AAAATTGGGCTTGAGTTCAT+GGG - Chr6:111935012-111935031 MsG0680035765.01.T01:CDS 35.0%
AATAGCAGCTATAGTGGTAT+TGG - Chr6:111934175-111934194 MsG0680035765.01.T01:intron 35.0%
AATTGTGATTGAGGTTGCAT+TGG - Chr6:111933609-111933628 MsG0680035765.01.T01:intron 35.0%
ACATAACACAACTCTTGGTT+TGG - Chr6:111933767-111933786 MsG0680035765.01.T01:intron 35.0%
CATAACACAACTCTTGGTTT+GGG - Chr6:111933768-111933787 MsG0680035765.01.T01:intron 35.0%
GTTTCACATAACACAACTCT+TGG - Chr6:111933762-111933781 MsG0680035765.01.T01:intron 35.0%
TAGTGTGATGCATATAGACA+GGG - Chr6:111933514-111933533 MsG0680035765.01.T01:intron 35.0%
TTAGTGTGATGCATATAGAC+AGG - Chr6:111933513-111933532 MsG0680035765.01.T01:intron 35.0%
! AAAAGCATGCAATCATAAGG+TGG + Chr6:111933065-111933084 None:intergenic 35.0%
! AAACACTTGACTCCTTGTTT+AGG + Chr6:111934689-111934708 None:intergenic 35.0%
! ATAACACAACTCTTGGTTTG+GGG - Chr6:111933769-111933788 MsG0680035765.01.T01:intron 35.0%
! CGTTGTTTAAAACTCTGCAT+AGG - Chr6:111933697-111933716 MsG0680035765.01.T01:intron 35.0%
! CTCACACTCATTATTTTCAC+CGG - Chr6:111933250-111933269 MsG0680035765.01.T01:CDS 35.0%
! TGGGGTAGAAGTTGATTTAT+TGG - Chr6:111933787-111933806 MsG0680035765.01.T01:intron 35.0%
! TTAGTTTGAGAGTCTGTCTT+TGG - Chr6:111934509-111934528 MsG0680035765.01.T01:intron 35.0%
!! ATGCAGCTCTTTGTTGATTT+CGG + Chr6:111934664-111934683 None:intergenic 35.0%
AAAAGAGTTGCACGTTCTTC+TGG - Chr6:111934539-111934558 MsG0680035765.01.T01:intron 40.0%
AAGCAGCGTGACACAAAAAT+TGG - Chr6:111934997-111935016 MsG0680035765.01.T01:CDS 40.0%
AATGATGCAAGTGAGCATGA+TGG - Chr6:111934632-111934651 MsG0680035765.01.T01:intron 40.0%
AGAGCTGCATTACCTAAACA+AGG - Chr6:111934674-111934693 MsG0680035765.01.T01:intron 40.0%
AGCAGCGTGACACAAAAATT+GGG - Chr6:111934998-111935017 MsG0680035765.01.T01:CDS 40.0%
CATGGATAGGAGACAAAAAG+TGG - Chr6:111933950-111933969 MsG0680035765.01.T01:intron 40.0%
TCATGCTCACTTGCATCATT+TGG + Chr6:111934632-111934651 None:intergenic 40.0%
! AATAAATAGACCTGATGGCG+AGG - Chr6:111935071-111935090 MsG0680035765.01.T01:CDS 40.0%
! GCAACACAATGCTTTTGATG+TGG - Chr6:111933571-111933590 MsG0680035765.01.T01:intron 40.0%
! GTAGTTTGTCCTCCTTGTTT+AGG + Chr6:111934960-111934979 None:intergenic 40.0%
! GTCACTGAGGTTAAGAGTTT+TGG - Chr6:111933167-111933186 MsG0680035765.01.T01:CDS 40.0%
! TATGGTGTGAAAAAGCGTGA+TGG - Chr6:111933206-111933225 MsG0680035765.01.T01:CDS 40.0%
!! TGCAGCTCTTTGTTGATTTC+GGG + Chr6:111934663-111934682 None:intergenic 40.0%
!!! AGTTTTGGAACGATGCAGTT+GGG - Chr6:111933182-111933201 MsG0680035765.01.T01:CDS 40.0%
AACGGTCAGTGTATCTGTTG+TGG + Chr6:111933681-111933700 None:intergenic 45.0%
AAGAAATGGGGTTGTCACTG+AGG - Chr6:111933154-111933173 MsG0680035765.01.T01:CDS 45.0%
ATGGTTCGATTCTGTAGCAC+AGG - Chr6:111933546-111933565 MsG0680035765.01.T01:intron 45.0%
CTGCATAGGTAGATGCGAAT+TGG - Chr6:111933711-111933730 MsG0680035765.01.T01:intron 45.0%
CTTGAGTTCATGGGAGATGA+AGG - Chr6:111935021-111935040 MsG0680035765.01.T01:CDS 45.0%
GCTGCATTACCTAAACAAGG+AGG - Chr6:111934677-111934696 MsG0680035765.01.T01:intron 45.0%
TATGATAACCTTGGCGCAAC+TGG - Chr6:111935126-111935145 MsG0680035765.01.T01:CDS 45.0%
!! CGTTCTTCTGGAACTTCGAA+TGG - Chr6:111934551-111934570 MsG0680035765.01.T01:intron 45.0%
!!! GAGTTTTGGAACGATGCAGT+TGG - Chr6:111933181-111933200 MsG0680035765.01.T01:CDS 45.0%
!! TATTTATTTATATATAATCT+AGG - Chr6:111933453-111933472 MsG0680035765.01.T01:intron 5.0%
AGGAGGACAAACTACTGCGT+TGG - Chr6:111934965-111934984 MsG0680035765.01.T01:intron 50.0%
CAACTTTACCAGTTGCGCCA+AGG + Chr6:111935137-111935156 None:intergenic 50.0%
CCTCTTCTAAATGCCCCATG+TGG - Chr6:111934128-111934147 MsG0680035765.01.T01:intron 50.0%
CGGTGTGTTCAAACTCCACT+AGG + Chr6:111934312-111934331 None:intergenic 50.0%
GCGTGATGGCAGATTCTATC+AGG - Chr6:111933220-111933239 MsG0680035765.01.T01:CDS 50.0%
GCTATAGCACTGTTGCCTAG+TGG - Chr6:111934294-111934313 MsG0680035765.01.T01:intron 50.0%
GGAACGATGCAGTTGGGTTA+TGG - Chr6:111933188-111933207 MsG0680035765.01.T01:CDS 50.0%
GGCGAGGATAGTGATACAGA+CGG - Chr6:111935087-111935106 MsG0680035765.01.T01:CDS 50.0%
GTATCACTATCCTCGCCATC+AGG + Chr6:111935084-111935103 None:intergenic 50.0%
! CCACATGGGGCATTTAGAAG+AGG + Chr6:111934131-111934150 None:intergenic 50.0%
!! CAAACTACTGCGTTGGCTAC+TGG - Chr6:111934972-111934991 MsG0680035765.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 111933047 111935167 111933047 ID=MsG0680035765.01;Name=MsG0680035765.01
Chr6 mRNA 111933047 111935167 111933047 ID=MsG0680035765.01.T01;Parent=MsG0680035765.01;Name=MsG0680035765.01.T01;_AED=0.45;_eAED=0.46;_QI=12|1|0.5|1|1|1|2|0|150
Chr6 exon 111933047 111933316 111933047 ID=MsG0680035765.01.T01:exon:9417;Parent=MsG0680035765.01.T01
Chr6 exon 111934973 111935167 111934973 ID=MsG0680035765.01.T01:exon:9416;Parent=MsG0680035765.01.T01
Chr6 five_prime_UTR 111935156 111935167 111935156 ID=MsG0680035765.01.T01:five_prime_utr;Parent=MsG0680035765.01.T01
Chr6 CDS 111934973 111935155 111934973 ID=MsG0680035765.01.T01:cds;Parent=MsG0680035765.01.T01
Chr6 CDS 111933047 111933316 111933047 ID=MsG0680035765.01.T01:cds;Parent=MsG0680035765.01.T01
Gene Sequence

>MsG0680035765.01.T01

ATGCCACCTTATGATTGCATGCTTTTGTTCAAGCCGAATGTGAAAAAAGAAGCTCTAATTGGTTTAGTTTCAAGGATTGGGAAACATGTGTGTAAAAGAAATGGGGTTGTCACTGAGGTTAAGAGTTTTGGAACGATGCAGTTGGGTTATGGTGTGAAAAAGCGTGATGGCAGATTCTATCAGGGCCAAATGATGCAAGTGAGCATGATGGCTACACCCGAAATCAACAAAGAGCTGCATTACCTAAACAAGGAGGACAAACTACTGCGTTGGCTACTGGTTAAGCAGCGTGACACAAAAATTGGGCTTGAGTTCATGGGAGATGAAGGTAGATTCGAGCTAAACAGATTCTCTGAAATAAATAGACCTGATGGCGAGGATAGTGATACAGACGGAGATGATGATGATGAATATGATAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGA

Protein sequence

>MsG0680035765.01.T01

MPPYDCMLLFKPNVKKEALIGLVSRIGKHVCKRNGVVTEVKSFGTMQLGYGVKKRDGRFYQGQMMQVSMMATPEINKELHYLNKEDKLLRWLLVKQRDTKIGLEFMGDEGRFELNRFSEINRPDGEDSDTDGDDDDEYDNLGATGKVVVM*