AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033081.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033081.01.T01 MTR_7g091500 61.714 175 41 3 1 175 310 458 3.96e-59 191
MsG0680033081.01.T02 MTR_7g091500 61.714 175 41 3 1 175 310 458 3.96e-59 191
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033081.01.T01 AT4G35910 37.572 173 82 3 1 173 310 456 2.72e-28 109
MsG0680033081.01.T02 AT4G35910 37.572 173 82 3 1 173 310 456 2.72e-28 109

Find 22 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATCTGTGAGAGTATTTA+AGG 0.114882 6:+55970211 MsG0680033081.01.T01:intron
AGAGTAGTTATTTGCAATTT+AGG 0.197365 6:+55970296 MsG0680033081.01.T01:three_prime_UTR
TCCTGGTTGTGGTAAATTTA+CGG 0.276660 6:+55970027 MsG0680033081.01.T01:three_prime_UTR
ACATTTGTGAGAGTATTTAA+AGG 0.283407 6:+55970149 MsG0680033081.01.T01:intron
TTATGAGAAGGTGTTGCTTT+TGG 0.302591 6:+55970060 MsG0680033081.01.T01:three_prime_UTR
ACACTGAGATTCTCAGTAAA+GGG 0.400133 6:+55969999 MsG0680033081.01.T01:three_prime_UTR
ATGAAGCATGTATTCTAGAT+TGG 0.448279 6:+55970367 MsG0680033081.01.T01:three_prime_UTR
TGGAGTTAACCACTTAGATA+TGG 0.463595 6:+55970105 MsG0680033081.01.T01:three_prime_UTR
CTCAGTAAAGGGGCAAATCC+TGG 0.465806 6:+55970010 MsG0680033081.01.T01:three_prime_UTR
TCATGCAATATCAAGAGTTA+TGG 0.489468 6:+55970085 MsG0680033081.01.T01:three_prime_UTR
CAAGATTGCTTGCTTTCAGA+TGG 0.509712 6:+55969948 MsG0680033081.01.T01:CDS
TACACTGAGATTCTCAGTAA+AGG 0.510030 6:+55969998 MsG0680033081.01.T01:three_prime_UTR
AACTACTCTCTTCATTGTCG+TGG 0.555301 6:-55970282 None:intergenic
AAAGGGGCAAATCCTGGTTG+TGG 0.603416 6:+55970016 MsG0680033081.01.T01:three_prime_UTR
GGTTTGACTGACTTATGAGA+AGG 0.603735 6:+55970048 MsG0680033081.01.T01:three_prime_UTR
TCAACACCACCATATCTAAG+TGG 0.612253 6:-55970114 None:intergenic
ACCGTAAATTTACCACAACC+AGG 0.624926 6:-55970028 None:intergenic
ATAACTCTTGATATTGCATG+AGG 0.626773 6:-55970083 None:intergenic
CACTGAGATTCTCAGTAAAG+GGG 0.636302 6:+55970000 MsG0680033081.01.T01:three_prime_UTR
TTTACTGAGAATCTCAGTGT+AGG 0.639224 6:-55969996 None:intergenic
AGTTAACCACTTAGATATGG+TGG 0.640695 6:+55970108 MsG0680033081.01.T01:three_prime_UTR
GCAGACATGAAACAACGACA+AGG 0.754505 6:-55970342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAAATTTAGTAATAACT+CGG + Chr6:55969427-55969446 MsG0680033081.01.T01:intron 15.0%
!!! TAATGTTTTTTGTTTAGATA+TGG + Chr6:55970173-55970192 MsG0680033081.01.T01:intron 15.0%
!! TAAAAGAAAACAGATTGAAT+TGG - Chr6:55969502-55969521 None:intergenic 20.0%
!! TATAATAAAAGATTCCATGA+AGG - Chr6:55969476-55969495 None:intergenic 20.0%
!!! AATTCAATCTGTTTTCTTTT+AGG + Chr6:55969501-55969520 MsG0680033081.01.T01:intron 20.0%
!!! ATCAAATAGATCATTTTGAT+AGG + Chr6:55969607-55969626 MsG0680033081.01.T01:intron 20.0%
!!! ATCAATGGAGAAATTTTATA+CGG + Chr6:55969299-55969318 MsG0680033081.01.T01:CDS 20.0%
!!! GAAATTTGAATAATCTGTTT+TGG + Chr6:55969565-55969584 MsG0680033081.01.T01:intron 20.0%
!!! TTTTGAGATTATATCTCTTT+TGG - Chr6:55969115-55969134 None:intergenic 20.0%
! AATGTGTCATACCAAAAAAT+AGG - Chr6:55969870-55969889 None:intergenic 25.0%
! AGAGTAGTTATTTGCAATTT+AGG + Chr6:55970296-55970315 MsG0680033081.01.T01:three_prime_UTR 25.0%
! ATAAAATTTCTCCATTGATG+TGG - Chr6:55969298-55969317 None:intergenic 25.0%
!! TTATTCCTTTTCAATTCAAC+AGG + Chr6:55969042-55969061 MsG0680033081.01.T01:CDS 25.0%
!!! ACATTTGTGAGAGTATTTAA+AGG + Chr6:55970149-55970168 MsG0680033081.01.T01:intron 25.0%
!!! ATGTGTTTTTTCATGTGTTT+AGG + Chr6:55969709-55969728 MsG0680033081.01.T01:intron 25.0%
!!! TAATGTTTTTTGTTCTGCAT+TGG + Chr6:55970235-55970254 MsG0680033081.01.T01:three_prime_UTR 25.0%
ATAACTCTTGATATTGCATG+AGG - Chr6:55970086-55970105 None:intergenic 30.0%
ATGAAAAAACACATTTACCG+CGG - Chr6:55969703-55969722 None:intergenic 30.0%
ATGAAGCATGTATTCTAGAT+TGG + Chr6:55970367-55970386 MsG0680033081.01.T01:three_prime_UTR 30.0%
TCATGCAATATCAAGAGTTA+TGG + Chr6:55970085-55970104 MsG0680033081.01.T01:three_prime_UTR 30.0%
TGAAGTTTCACTACAAGAAA+TGG - Chr6:55969647-55969666 None:intergenic 30.0%
! ATGATCGATTTATTGTGACT+TGG + Chr6:55969809-55969828 MsG0680033081.01.T01:intron 30.0%
! TGATCGATTTATTGTGACTT+GGG + Chr6:55969810-55969829 MsG0680033081.01.T01:intron 30.0%
! TTCATTAAGATCTTCTAGCT+GGG + Chr6:55969663-55969682 MsG0680033081.01.T01:CDS 30.0%
!! AACATCTGTGAGAGTATTTA+AGG + Chr6:55970211-55970230 MsG0680033081.01.T01:intron 30.0%
!! CGACACGTAAAATTTGTTTT+TGG - Chr6:55969902-55969921 None:intergenic 30.0%
!!! GAAAGAGTCTACCTATTTTT+TGG + Chr6:55969856-55969875 MsG0680033081.01.T01:intron 30.0%
!!! TCTGCATTGGTTGTATTTTA+TGG + Chr6:55970248-55970267 MsG0680033081.01.T01:three_prime_UTR 30.0%
!!! TGTCGATATTCTTGTTTTCA+GGG + Chr6:55969917-55969936 MsG0680033081.01.T01:intron 30.0%
AACATTACCATCAACGATCT+CGG - Chr6:55969072-55969091 None:intergenic 35.0%
AATTGTGCTCCTACTAAAGA+AGG - Chr6:55969780-55969799 None:intergenic 35.0%
ACACTGAGATTCTCAGTAAA+GGG + Chr6:55969999-55970018 MsG0680033081.01.T01:three_prime_UTR 35.0%
AGTTAACCACTTAGATATGG+TGG + Chr6:55970108-55970127 MsG0680033081.01.T01:three_prime_UTR 35.0%
ATGGCAATTTGAATGCGTTA+AGG + Chr6:55969364-55969383 MsG0680033081.01.T01:CDS 35.0%
ATTGTGCTCCTACTAAAGAA+GGG - Chr6:55969779-55969798 None:intergenic 35.0%
CAATTTGAATGCGTTAAGGT+GGG + Chr6:55969368-55969387 MsG0680033081.01.T01:CDS 35.0%
CCAAAAGTGAGATTGTTGAT+TGG + Chr6:55969189-55969208 MsG0680033081.01.T01:CDS 35.0%
CTTCATTAAGATCTTCTAGC+TGG + Chr6:55969662-55969681 MsG0680033081.01.T01:CDS 35.0%
GGAATCCTGTTGAATTGAAA+AGG - Chr6:55969050-55969069 None:intergenic 35.0%
TACACTGAGATTCTCAGTAA+AGG + Chr6:55969998-55970017 MsG0680033081.01.T01:three_prime_UTR 35.0%
TCCTGGTTGTGGTAAATTTA+CGG + Chr6:55970027-55970046 MsG0680033081.01.T01:three_prime_UTR 35.0%
TGGAGTTAACCACTTAGATA+TGG + Chr6:55970105-55970124 MsG0680033081.01.T01:three_prime_UTR 35.0%
TTGAGTGATGGTAAGAAACT+AGG + Chr6:55969535-55969554 MsG0680033081.01.T01:intron 35.0%
TTTACTGAGAATCTCAGTGT+AGG - Chr6:55969999-55970018 None:intergenic 35.0%
! CCAATCAACAATCTCACTTT+TGG - Chr6:55969192-55969211 None:intergenic 35.0%
! TCAAATTGCCATTGTTGACT+CGG - Chr6:55969356-55969375 None:intergenic 35.0%
! TTCTGAGGAAACAGATTCAT+TGG - Chr6:55969138-55969157 None:intergenic 35.0%
! TTCTTTTGCCCTTCTTTAGT+AGG + Chr6:55969768-55969787 MsG0680033081.01.T01:intron 35.0%
!!! GTGTCGATATTCTTGTTTTC+AGG + Chr6:55969916-55969935 MsG0680033081.01.T01:intron 35.0%
!!! TTATGAGAAGGTGTTGCTTT+TGG + Chr6:55970060-55970079 MsG0680033081.01.T01:three_prime_UTR 35.0%
AACTACTCTCTTCATTGTCG+TGG - Chr6:55970285-55970304 None:intergenic 40.0%
ACATTACCATCAACGATCTC+GGG - Chr6:55969071-55969090 None:intergenic 40.0%
ACCGTAAATTTACCACAACC+AGG - Chr6:55970031-55970050 None:intergenic 40.0%
AGAAGGGCAAAAGAACATCA+TGG - Chr6:55969763-55969782 None:intergenic 40.0%
AGCACAATTGTAAGAACAGC+TGG + Chr6:55969014-55969033 MsG0680033081.01.T01:CDS 40.0%
ATAACTCGGTCTAAGCCAAT+TGG + Chr6:55969441-55969460 MsG0680033081.01.T01:intron 40.0%
CAAGATTGCTTGCTTTCAGA+TGG + Chr6:55969948-55969967 MsG0680033081.01.T01:CDS 40.0%
CACTGAGATTCTCAGTAAAG+GGG + Chr6:55970000-55970019 MsG0680033081.01.T01:three_prime_UTR 40.0%
GCAATTTGAATGCGTTAAGG+TGG + Chr6:55969367-55969386 MsG0680033081.01.T01:CDS 40.0%
GCACAATTGTAAGAACAGCT+GGG + Chr6:55969015-55969034 MsG0680033081.01.T01:CDS 40.0%
TACGGATTTACCTCATTCAG+TGG + Chr6:55969317-55969336 MsG0680033081.01.T01:CDS 40.0%
TCAACACCACCATATCTAAG+TGG - Chr6:55970117-55970136 None:intergenic 40.0%
TTCACTACAAGAAATGGCCA+TGG - Chr6:55969641-55969660 None:intergenic 40.0%
TTCCACAGATACCACATCAA+TGG + Chr6:55969284-55969303 MsG0680033081.01.T01:CDS 40.0%
TTTACCGCGGAAAAAATGCA+AGG - Chr6:55969690-55969709 None:intergenic 40.0%
! GGTTTGACTGACTTATGAGA+AGG + Chr6:55970048-55970067 MsG0680033081.01.T01:three_prime_UTR 40.0%
! TTAGTAGGAGCACAATTGCT+TGG + Chr6:55969783-55969802 MsG0680033081.01.T01:intron 40.0%
!! ACAATCTCACTTTTGGCAAG+TGG - Chr6:55969185-55969204 None:intergenic 40.0%
!! CGTTGATGGTAATGTTCCAT+TGG + Chr6:55969076-55969095 MsG0680033081.01.T01:CDS 40.0%
!! GTAACTTTGCTGTTGAGTGA+TGG + Chr6:55969523-55969542 MsG0680033081.01.T01:intron 40.0%
AAGAAATGGCCATGGTCTCA+AGG - Chr6:55969633-55969652 None:intergenic 45.0%
ACAATTGTGCTCTCTCGAGA+CGG - Chr6:55969005-55969024 None:intergenic 45.0%
CTCCATTGATGTGGTATCTG+TGG - Chr6:55969289-55969308 None:intergenic 45.0%
GAGAGGACAGAAAGATTCTG+AGG - Chr6:55969153-55969172 None:intergenic 45.0%
GCAGACATGAAACAACGACA+AGG - Chr6:55970345-55970364 None:intergenic 45.0%
GTCTAAGCCAATTGGCAACT+GGG + Chr6:55969449-55969468 MsG0680033081.01.T01:intron 45.0%
TACCTCATTCAGTGGTTGCA+CGG + Chr6:55969325-55969344 MsG0680033081.01.T01:CDS 45.0%
TTTGATAGGCCTTGAGACCA+TGG + Chr6:55969621-55969640 MsG0680033081.01.T01:intron 45.0%
AAAGGGGCAAATCCTGGTTG+TGG + Chr6:55970016-55970035 MsG0680033081.01.T01:three_prime_UTR 50.0%
ACCTCATTCAGTGGTTGCAC+GGG + Chr6:55969326-55969345 MsG0680033081.01.T01:CDS 50.0%
AGGATTCCCGAGATCGTTGA+TGG + Chr6:55969062-55969081 MsG0680033081.01.T01:CDS 50.0%
ATTGGCAACTGGGTCCTTCA+TGG + Chr6:55969459-55969478 MsG0680033081.01.T01:intron 50.0%
CTCAGTAAAGGGGCAAATCC+TGG + Chr6:55970010-55970029 MsG0680033081.01.T01:three_prime_UTR 50.0%
GGTCTAAGCCAATTGGCAAC+TGG + Chr6:55969448-55969467 MsG0680033081.01.T01:intron 50.0%
TGAAGGACCCAGTTGCCAAT+TGG - Chr6:55969459-55969478 None:intergenic 50.0%
!! GCAAGTGGACTATTGCAGAG+AGG - Chr6:55969170-55969189 None:intergenic 50.0%
!!! GGGTCCTTGCATTTTTTCCG+CGG + Chr6:55969683-55969702 MsG0680033081.01.T01:CDS 50.0%
CGGGCAAACCGAGTCAACAA+TGG + Chr6:55969345-55969364 MsG0680033081.01.T01:CDS 55.0%
!! TGGCGTCTTCGAGTTGCCAA+TGG - Chr6:55969095-55969114 None:intergenic 55.0%
GCCCGTGCAACCACTGAATG+AGG - Chr6:55969330-55969349 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 55968987 55970452 55968987 ID=MsG0680033081.01;Name=MsG0680033081.01
Chr6 mRNA 55968987 55970452 55968987 ID=MsG0680033081.01.T01;Parent=MsG0680033081.01;Name=MsG0680033081.01.T01;_AED=0.40;_eAED=0.45;_QI=0|0.33|0.5|1|0|0.25|4|403|175
Chr6 exon 55970218 55970452 55970218 ID=MsG0680033081.01.T01:exon:16964;Parent=MsG0680033081.01.T01
Chr6 exon 55969938 55970154 55969938 ID=MsG0680033081.01.T01:exon:16963;Parent=MsG0680033081.01.T01
Chr6 CDS 55968987 55969389 55968987 ID=MsG0680033081.01.T01:cds;Parent=MsG0680033081.01.T01
Chr6 CDS 55969629 55969704 55969629 ID=MsG0680033081.01.T01:cds;Parent=MsG0680033081.01.T01
Chr6 CDS 55969938 55969986 55969938 ID=MsG0680033081.01.T01:cds;Parent=MsG0680033081.01.T01
Chr6 three_prime_UTR 55969987 55970154 55969987 ID=MsG0680033081.01.T01:three_prime_utr;Parent=MsG0680033081.01.T01
Chr6 three_prime_UTR 55970218 55970452 55970218 ID=MsG0680033081.01.T01:three_prime_utr;Parent=MsG0680033081.01.T01
Chr6 mRNA 55968987 55970426 55968987 ID=MsG0680033081.01.T02;Parent=MsG0680033081.01;Name=MsG0680033081.01.T02;_AED=0.40;_eAED=0.45;_QI=0|0.33|0.5|1|0|0.25|4|397|175
Chr6 exon 55970233 55970426 55970233 ID=MsG0680033081.01.T02:exon:16966;Parent=MsG0680033081.01.T02
Chr6 exon 55969938 55970189 55969938 ID=MsG0680033081.01.T02:exon:16965;Parent=MsG0680033081.01.T02
Chr6 CDS 55968987 55969389 55968987 ID=MsG0680033081.01.T02:cds;Parent=MsG0680033081.01.T02
Chr6 CDS 55969629 55969704 55969629 ID=MsG0680033081.01.T02:cds;Parent=MsG0680033081.01.T02
Chr6 CDS 55969938 55969986 55969938 ID=MsG0680033081.01.T02:cds;Parent=MsG0680033081.01.T02
Chr6 three_prime_UTR 55969987 55970189 55969987 ID=MsG0680033081.01.T02:three_prime_utr;Parent=MsG0680033081.01.T02
Chr6 three_prime_UTR 55970233 55970426 55970233 ID=MsG0680033081.01.T02:three_prime_utr;Parent=MsG0680033081.01.T02
Gene Sequence

>MsG0680033081.01.T01

TTGCAGGAAGAGAATCCGTCTCGAGAGAGCACAATTGTAAGAACAGCTGGGAAACTTATTCCTTTTCAATTCAACAGGATTCCCGAGATCGTTGATGGTAATGTTCCATTGGCAACTCGAAGACGCCAAAAGAGATATAATCTCAAAACCAATGAATCTGTTTCCTCAGAATCTTTCTGTCCTCTCTGCAATAGTCCACTTGCCAAAAGTGAGATTGTTGATTGGAGCAATCACAATAACTGCAAAAGTAGTGACACTTTTATACTTTGTGCTGTTCAAGTTGTCAGTTTCAGATACTTCCACAGATACCACATCAATGGAGAAATTTTATACGGATTTACCTCATTCAGTGGTTGCACGGGCAAACCGAGTCAACAATGGCAATTTGAATGCGTTAAGGTGGGCCTTGAGACCATGGCCATTTCTTGTAGTGAAACTTCATTAAGATCTTCTAGCTGGGTCCTTGCATTTTTTCCGCGGGAACAACTACAAGATTGCTTGCTTTCAGATGGTGAAGATGAAACTTAA

>MsG0680033081.01.T02

TTGCAGGAAGAGAATCCGTCTCGAGAGAGCACAATTGTAAGAACAGCTGGGAAACTTATTCCTTTTCAATTCAACAGGATTCCCGAGATCGTTGATGGTAATGTTCCATTGGCAACTCGAAGACGCCAAAAGAGATATAATCTCAAAACCAATGAATCTGTTTCCTCAGAATCTTTCTGTCCTCTCTGCAATAGTCCACTTGCCAAAAGTGAGATTGTTGATTGGAGCAATCACAATAACTGCAAAAGTAGTGACACTTTTATACTTTGTGCTGTTCAAGTTGTCAGTTTCAGATACTTCCACAGATACCACATCAATGGAGAAATTTTATACGGATTTACCTCATTCAGTGGTTGCACGGGCAAACCGAGTCAACAATGGCAATTTGAATGCGTTAAGGTGGGCCTTGAGACCATGGCCATTTCTTGTAGTGAAACTTCATTAAGATCTTCTAGCTGGGTCCTTGCATTTTTTCCGCGGGAACAACTACAAGATTGCTTGCTTTCAGATGGTGAAGATGAAACTTAA

Protein sequence

>MsG0680033081.01.T01

LQEENPSRESTIVRTAGKLIPFQFNRIPEIVDGNVPLATRRRQKRYNLKTNESVSSESFCPLCNSPLAKSEIVDWSNHNNCKSSDTFILCAVQVVSFRYFHRYHINGEILYGFTSFSGCTGKPSQQWQFECVKVGLETMAISCSETSLRSSSWVLAFFPREQLQDCLLSDGEDET*

>MsG0680033081.01.T02

LQEENPSRESTIVRTAGKLIPFQFNRIPEIVDGNVPLATRRRQKRYNLKTNESVSSESFCPLCNSPLAKSEIVDWSNHNNCKSSDTFILCAVQVVSFRYFHRYHINGEILYGFTSFSGCTGKPSQQWQFECVKVGLETMAISCSETSLRSSSWVLAFFPREQLQDCLLSDGEDET*