Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000028.01 | MsG0780036258.01 | 0.802925 | 4.451802e-49 | 2.207918e-46 |
| MsG0180004059.01 | MsG0780036258.01 | 0.815367 | 9.585481e-52 | 6.595902e-49 |
| MsG0180005060.01 | MsG0780036258.01 | 0.816483 | 5.403024e-52 | 3.833051e-49 |
| MsG0180006253.01 | MsG0780036258.01 | 0.842018 | 3.339758e-58 | 4.997137e-55 |
| MsG0280006316.01 | MsG0780036258.01 | 0.826732 | 2.308750e-54 | 2.183374e-51 |
| MsG0280010623.01 | MsG0780036258.01 | 0.802719 | 4.908956e-49 | 2.421999e-46 |
| MsG0280011017.01 | MsG0780036258.01 | 0.814410 | 1.562552e-51 | 1.047743e-48 |
| MsG0380012222.01 | MsG0780036258.01 | 0.803936 | 2.747114e-49 | 1.398395e-46 |
| MsG0380013213.01 | MsG0780036258.01 | 0.802499 | 5.450416e-49 | 2.673971e-46 |
| MsG0380013226.01 | MsG0780036258.01 | 0.806939 | 6.446799e-50 | 3.544503e-47 |
| MsG0680032026.01 | MsG0780036258.01 | 0.805396 | 1.362323e-49 | 7.199468e-47 |
| MsG0680034974.01 | MsG0780036258.01 | 0.806498 | 7.989027e-50 | 4.342830e-47 |
| MsG0680034978.01 | MsG0780036258.01 | 0.804104 | 2.535145e-49 | 1.295974e-46 |
| MsG0480018559.01 | MsG0780036258.01 | 0.821092 | 4.850821e-53 | 3.908837e-50 |
| MsG0480018617.01 | MsG0780036258.01 | 0.804789 | 1.824842e-49 | 9.494899e-47 |
| MsG0480020904.01 | MsG0780036258.01 | 0.805091 | 1.578145e-49 | 8.275087e-47 |
| MsG0480021091.01 | MsG0780036258.01 | 0.801824 | 7.504267e-49 | 3.618957e-46 |
| MsG0480021234.01 | MsG0780036258.01 | 0.813515 | 2.462010e-51 | 1.611182e-48 |
| MsG0580024994.01 | MsG0780036258.01 | 0.831494 | 1.616792e-55 | 1.757434e-52 |
| MsG0580025007.01 | MsG0780036258.01 | 0.805939 | 1.047952e-49 | 5.616118e-47 |
| MsG0580025024.01 | MsG0780036258.01 | 0.831180 | 1.931746e-55 | 2.080168e-52 |
| MsG0380017168.01 | MsG0780036258.01 | 0.801645 | 8.166143e-49 | 3.920173e-46 |
| MsG0380017200.01 | MsG0780036258.01 | 0.808443 | 3.090200e-50 | 1.767191e-47 |
| MsG0480018439.01 | MsG0780036258.01 | 0.800361 | 1.493977e-48 | 6.943074e-46 |
| MsG0280007874.01 | MsG0780036258.01 | 0.803975 | 2.697443e-49 | 1.374404e-46 |
| MsG0280010425.01 | MsG0780036258.01 | 0.820881 | 5.425888e-53 | 4.346554e-50 |
| MsG0780035930.01 | MsG0780036258.01 | 0.802581 | 5.240714e-49 | 2.576515e-46 |
| MsG0780036258.01 | MsG0780036259.01 | 0.930673 | 1.161976e-93 | 6.996842e-89 |
| MsG0780036258.01 | MsG0780040670.01 | 0.816133 | 6.470883e-52 | 4.547040e-49 |
| MsG0780036258.01 | MsG0780040716.01 | 0.812051 | 5.149852e-51 | 3.240150e-48 |
| MsG0780036258.01 | MsG0780041447.01 | 0.825669 | 4.131480e-54 | 3.790773e-51 |
| MsG0780036258.01 | MsG0880042633.01 | 0.831959 | 1.242103e-55 | 1.368947e-52 |
| MsG0780036258.01 | MsG0880042728.01 | 0.820814 | 5.620751e-53 | 4.494181e-50 |
| MsG0780036258.01 | MsG0880045854.01 | 0.806060 | 9.881721e-50 | 5.312174e-47 |
| MsG0780036258.01 | MsG0880046923.01 | 0.810275 | 1.249712e-50 | 7.500619e-48 |
| MsG0780036258.01 | MsG0880047312.01 | 0.801551 | 8.536446e-49 | 4.088238e-46 |
| MsG0780036258.01 | MsG0880047450.01 | 0.825138 | 5.520398e-54 | 4.989282e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGAAGGAATGCGCGAC+CGG | 0.412108 | 7:+5455381 | MsG0780036258.01.T01:CDS |
| GGAATGCGCGACCGGGTGGC+TGG | 0.413441 | 7:+5455389 | MsG0780036258.01.T01:CDS |
| ATGAGATATTGTGACATACT+TGG | 0.456754 | 7:+5455444 | MsG0780036258.01.T01:CDS |
| AAGAAAATTCAAAACATTCA+AGG | 0.456837 | 7:-5455485 | None:intergenic |
| ATGACCACATACGTCATAGA+AGG | 0.503651 | 7:+5455360 | MsG0780036258.01.T01:CDS |
| GGATGTAAACGAGATGGATC+GGG | 0.521846 | 7:+5455410 | MsG0780036258.01.T01:CDS |
| TGGATGTAAACGAGATGGAT+CGG | 0.553901 | 7:+5455409 | MsG0780036258.01.T01:CDS |
| GACATACTTGGAGTTCGATA+CGG | 0.558585 | 7:+5455456 | MsG0780036258.01.T01:CDS |
| CTCGTTTACATCCAGCCACC+CGG | 0.571779 | 7:-5455400 | None:intergenic |
| GCTGGAAGGAATGCGCGACC+GGG | 0.573064 | 7:+5455382 | MsG0780036258.01.T01:CDS |
| GGAAGGAATGCGCGACCGGG+TGG | 0.575460 | 7:+5455385 | MsG0780036258.01.T01:CDS |
| CCACATACGTCATAGAAGGC+TGG | 0.581811 | 7:+5455364 | MsG0780036258.01.T01:CDS |
| CCAGCCTTCTATGACGTATG+TGG | 0.595690 | 7:-5455364 | None:intergenic |
| GTGGCTGGATGTAAACGAGA+TGG | 0.595690 | 7:+5455404 | MsG0780036258.01.T01:CDS |
| AGGCAGACCAGATCAAGAGA+TGG | 0.637775 | 7:-5455514 | None:intergenic |
| GTAAACGAGATGGATCGGGT+TGG | 0.643330 | 7:+5455414 | MsG0780036258.01.T01:CDS |
| ATACGTCATAGAAGGCTGGA+AGG | 0.655440 | 7:+5455368 | MsG0780036258.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAATTCAAAACATTCA+AGG | - | Chr7:5455488-5455507 | None:intergenic | 20.0% |
| ATGAGATATTGTGACATACT+TGG | + | Chr7:5455444-5455463 | MsG0780036258.01.T01:CDS | 30.0% | |
| ATGACCACATACGTCATAGA+AGG | + | Chr7:5455360-5455379 | MsG0780036258.01.T01:CDS | 40.0% | |
| GACATACTTGGAGTTCGATA+CGG | + | Chr7:5455456-5455475 | MsG0780036258.01.T01:CDS | 40.0% | |
| TGGATGTAAACGAGATGGAT+CGG | + | Chr7:5455409-5455428 | MsG0780036258.01.T01:CDS | 40.0% | |
| ATACGTCATAGAAGGCTGGA+AGG | + | Chr7:5455368-5455387 | MsG0780036258.01.T01:CDS | 45.0% | |
| GGATGTAAACGAGATGGATC+GGG | + | Chr7:5455410-5455429 | MsG0780036258.01.T01:CDS | 45.0% | |
| CCACATACGTCATAGAAGGC+TGG | + | Chr7:5455364-5455383 | MsG0780036258.01.T01:CDS | 50.0% | |
| CCAGCCTTCTATGACGTATG+TGG | - | Chr7:5455367-5455386 | None:intergenic | 50.0% | |
| GTAAACGAGATGGATCGGGT+TGG | + | Chr7:5455414-5455433 | MsG0780036258.01.T01:CDS | 50.0% | |
| GTGGCTGGATGTAAACGAGA+TGG | + | Chr7:5455404-5455423 | MsG0780036258.01.T01:CDS | 50.0% | |
| CTCGTTTACATCCAGCCACC+CGG | - | Chr7:5455403-5455422 | None:intergenic | 55.0% | |
| GCTGGAAGGAATGCGCGACC+GGG | + | Chr7:5455382-5455401 | MsG0780036258.01.T01:CDS | 65.0% | |
| GGCTGGAAGGAATGCGCGAC+CGG | + | Chr7:5455381-5455400 | MsG0780036258.01.T01:CDS | 65.0% | |
| GGAAGGAATGCGCGACCGGG+TGG | + | Chr7:5455385-5455404 | MsG0780036258.01.T01:CDS | 70.0% | |
| GGAATGCGCGACCGGGTGGC+TGG | + | Chr7:5455389-5455408 | MsG0780036258.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 5455360 | 5455524 | 5455360 | ID=MsG0780036258.01;Name=MsG0780036258.01 |
| Chr7 | mRNA | 5455360 | 5455524 | 5455360 | ID=MsG0780036258.01.T01;Parent=MsG0780036258.01;Name=MsG0780036258.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|54 |
| Chr7 | exon | 5455360 | 5455524 | 5455360 | ID=MsG0780036258.01.T01:exon:32127;Parent=MsG0780036258.01.T01 |
| Chr7 | CDS | 5455360 | 5455524 | 5455360 | ID=MsG0780036258.01.T01:cds;Parent=MsG0780036258.01.T01 |
| Gene Sequence |
| Protein sequence |
