AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013226.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013226.01.T01 MTR_3g436100 81.250 64 12 0 1 64 73 136 9.26e-36 118
MsG0380013226.01.T01 MTR_7g044980 69.492 59 18 0 2 60 64 122 7.98e-28 97.8
MsG0380013226.01.T01 MTR_7g044920 63.333 60 22 0 1 60 70 129 6.36e-26 93.2
MsG0380013226.01.T01 MTR_6g017170 71.429 56 15 1 1 55 68 123 1.40e-25 91.7
MsG0380013226.01.T01 MTR_8g022400 61.765 68 24 2 2 67 69 136 3.89e-25 90.9
MsG0380013226.01.T01 MTR_3g067437 66.102 59 20 0 2 60 69 127 4.26e-25 90.5
MsG0380013226.01.T01 MTR_8g022420 69.643 56 16 1 2 56 68 123 3.41e-24 88.6
MsG0380013226.01.T01 MTR_8g022430 67.857 56 17 1 1 55 68 123 1.53e-23 86.7
MsG0380013226.01.T01 MTR_6g017150 56.338 71 24 2 1 70 68 132 8.62e-22 82.4
MsG0380013226.01.T01 MTR_3g067500 56.667 60 26 0 1 60 46 105 2.68e-21 80.5
MsG0380013226.01.T01 MTR_7g056803 56.250 64 27 1 5 67 74 137 4.79e-21 80.5
MsG0380013226.01.T01 MTR_7g056817 59.259 54 22 0 1 54 71 124 1.12e-20 79.3
MsG0380013226.01.T01 MTR_3g467760 50.746 67 32 1 2 67 43 109 1.81e-20 78.2
MsG0380013226.01.T01 MTR_8g056800 52.542 59 28 0 2 60 21 79 2.06e-20 77.8
MsG0380013226.01.T01 MTR_3g067580 54.237 59 27 0 2 60 77 135 2.56e-20 78.6
MsG0380013226.01.T01 MTR_3g067570 67.391 46 15 0 2 47 45 90 3.49e-20 77.0
MsG0380013226.01.T01 MTR_3g067270 50.746 67 32 1 2 67 67 133 4.14e-20 77.8
MsG0380013226.01.T01 MTR_3g467750 50.746 67 32 1 2 67 67 133 4.14e-20 77.8
MsG0380013226.01.T01 MTR_3g067280 50.746 67 32 1 2 67 67 133 4.87e-20 77.8
MsG0380013226.01.T01 MTR_3g067430 57.692 52 22 0 4 55 75 126 1.66e-19 76.6
MsG0380013226.01.T01 MTR_4g026590 52.174 69 32 1 1 68 75 143 2.00e-19 76.3
MsG0380013226.01.T01 MTR_3g067510 67.391 46 15 0 2 47 75 120 2.92e-19 75.9
MsG0380013226.01.T01 MTR_3g067510 67.391 46 15 0 2 47 70 115 3.24e-19 75.5
MsG0380013226.01.T01 MTR_3g463570 56.364 55 23 1 2 55 68 122 5.86e-19 75.1
MsG0380013226.01.T01 MTR_6g036620 61.538 52 20 0 1 52 66 117 6.16e-19 74.7
MsG0380013226.01.T01 MTR_3g463570 57.407 54 22 1 2 54 88 141 1.62e-18 74.3
MsG0380013226.01.T01 MTR_8g461020 50.794 63 28 1 1 60 67 129 6.25e-18 72.4
MsG0380013226.01.T01 MTR_3g067555 53.704 54 25 0 2 55 60 113 1.09e-17 71.6
MsG0380013226.01.T01 MTR_3g067540 53.704 54 25 0 2 55 73 126 1.10e-17 72.0
MsG0380013226.01.T01 MTR_3g067555 53.704 54 25 0 2 55 73 126 1.10e-17 72.0
MsG0380013226.01.T01 MTR_3g067550 53.704 54 25 0 2 55 52 105 1.16e-17 71.2
MsG0380013226.01.T01 MTR_3g067555 53.704 54 25 0 2 55 52 105 1.16e-17 71.2
MsG0380013226.01.T01 MTR_6g047880 48.276 58 30 0 1 58 69 126 4.08e-17 70.5
MsG0380013226.01.T01 MTR_3g067535 59.259 54 20 1 2 55 71 122 1.56e-16 68.9
MsG0380013226.01.T01 MTR_6g047900 47.458 59 30 1 1 58 65 123 1.68e-16 68.6
MsG0380013226.01.T01 MTR_1g019650 54.167 48 22 0 5 52 60 107 5.37e-16 66.6
MsG0380013226.01.T01 MTR_3g067445 55.556 45 20 0 2 46 73 117 8.40e-16 66.2
MsG0380013226.01.T01 MTR_7g029540 40.385 52 31 0 1 52 69 120 3.25e-12 57.4
MsG0380013226.01.T01 MTR_6g082060 62.500 40 15 0 19 58 91 130 5.62e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATGCTGATATTGAATA+TGG 0.285356 3:+33242425 MsG0380013226.01.T01:CDS
GCAGAACGTTACTTCAAGAT+TGG 0.399752 3:+33242473 MsG0380013226.01.T01:CDS
CCATATTCAATATCAGCATT+AGG 0.437256 3:-33242425 None:intergenic
ACTTCCCTTCTTGATGCACT+CGG 0.528289 3:-33242381 None:intergenic
GAGTGCATCAAGAAGGGAAG+TGG 0.546238 3:+33242383 MsG0380013226.01.T01:CDS
TCATGCCGAGTGCATCAAGA+AGG 0.559241 3:+33242376 MsG0380013226.01.T01:CDS
ACGTTCTGCTTCATTTACAG+AGG 0.615491 3:-33242459 None:intergenic
CATGCCGAGTGCATCAAGAA+GGG 0.625240 3:+33242377 MsG0380013226.01.T01:CDS
ATGCTGATATTGAATATGGA+TGG 0.639133 3:+33242429 MsG0380013226.01.T01:CDS
ACATAATATGAAGATGATCG+AGG 0.692790 3:+33242331 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAGAATTTTTTACTACA+GGG - Chr3:33242505-33242524 None:intergenic 20.0%
!!! CTTGAAGAATTTTTTACTAC+AGG - Chr3:33242506-33242525 None:intergenic 25.0%
ATGCTGATATTGAATATGGA+TGG + Chr3:33242429-33242448 MsG0380013226.01.T01:CDS 30.0%
CCATATTCAATATCAGCATT+AGG - Chr3:33242428-33242447 None:intergenic 30.0%
CCTAATGCTGATATTGAATA+TGG + Chr3:33242425-33242444 MsG0380013226.01.T01:CDS 30.0%
!! GTCTTTTTCATTAGAGTTTG+AGG - Chr3:33242533-33242552 None:intergenic 30.0%
!!! AGAATTTTTTACTACAGGGT+TGG - Chr3:33242501-33242520 None:intergenic 30.0%
! GCATGAGTTTTGCATAAGTT+AGG - Chr3:33242362-33242381 None:intergenic 35.0%
ACGTTCTGCTTCATTTACAG+AGG - Chr3:33242462-33242481 None:intergenic 40.0%
GCAGAACGTTACTTCAAGAT+TGG + Chr3:33242473-33242492 MsG0380013226.01.T01:CDS 40.0%
ACTTCCCTTCTTGATGCACT+CGG - Chr3:33242384-33242403 None:intergenic 45.0%
CATGCCGAGTGCATCAAGAA+GGG + Chr3:33242377-33242396 MsG0380013226.01.T01:CDS 50.0%
GAGTGCATCAAGAAGGGAAG+TGG + Chr3:33242383-33242402 MsG0380013226.01.T01:CDS 50.0%
TCATGCCGAGTGCATCAAGA+AGG + Chr3:33242376-33242395 MsG0380013226.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 33242338 33242559 33242338 ID=MsG0380013226.01;Name=MsG0380013226.01
Chr3 mRNA 33242338 33242559 33242338 ID=MsG0380013226.01.T01;Parent=MsG0380013226.01;Name=MsG0380013226.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|73
Chr3 exon 33242338 33242559 33242338 ID=MsG0380013226.01.T01:exon:6218;Parent=MsG0380013226.01.T01
Chr3 CDS 33242338 33242559 33242338 ID=MsG0380013226.01.T01:cds;Parent=MsG0380013226.01.T01
Gene Sequence

>MsG0380013226.01.T01

ATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCCGAGTGCATCAAGAAGGGAAGTGGAAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTGTTTTTCCTCTGTAAATGAAGCAGAACGTTACTTCAAGATTGGATCCAACCCTGTAGTAAAAAATTCTTCAAGATTGCCTCAAACTCTAATGAAAAAGACTACTTGA

Protein sequence

>MsG0380013226.01.T01

MKMIEEHPNLCKTHAECIKKGSGNFCARYPNADIEYGWCFSSVNEAERYFKIGSNPVVKNSSRLPQTLMKKTT*