AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013213.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013213.01.T01 MTR_3g436100 82.927 82 14 0 1 82 73 154 5.37e-47 147
MsG0380013213.01.T01 MTR_7g044920 62.651 83 30 1 1 82 70 152 1.96e-34 115
MsG0380013213.01.T01 MTR_7g044980 62.195 82 30 1 2 82 64 145 6.46e-33 111
MsG0380013213.01.T01 MTR_8g056800 53.086 81 38 0 2 82 21 101 1.87e-27 95.9
MsG0380013213.01.T01 MTR_6g017170 69.643 56 16 1 1 55 68 123 5.29e-24 87.8
MsG0380013213.01.T01 MTR_3g067437 64.407 59 21 0 2 60 69 127 6.09e-24 87.8
MsG0380013213.01.T01 MTR_8g022400 66.071 56 18 1 2 56 69 124 4.66e-23 86.3
MsG0380013213.01.T01 MTR_8g022420 60.938 64 24 1 2 64 68 131 1.33e-22 84.7
MsG0380013213.01.T01 MTR_8g022430 57.576 66 26 2 1 64 68 133 4.59e-22 83.6
MsG0380013213.01.T01 MTR_3g067500 54.839 62 28 0 1 62 46 107 9.05e-22 82.0
MsG0380013213.01.T01 MTR_6g017150 54.545 77 23 2 1 76 68 133 2.93e-21 81.3
MsG0380013213.01.T01 MTR_3g067580 52.308 65 30 1 2 65 77 141 3.14e-20 79.0
MsG0380013213.01.T01 MTR_3g067540 48.052 77 29 1 2 78 73 138 4.34e-20 78.2
MsG0380013213.01.T01 MTR_3g067555 48.052 77 29 1 2 78 73 138 4.34e-20 78.2
MsG0380013213.01.T01 MTR_3g467760 50.000 68 33 1 2 68 43 110 5.24e-20 77.4
MsG0380013213.01.T01 MTR_3g067555 48.052 77 29 1 2 78 60 125 5.60e-20 77.8
MsG0380013213.01.T01 MTR_3g067550 48.052 77 29 1 2 78 52 117 6.01e-20 77.4
MsG0380013213.01.T01 MTR_3g067555 48.052 77 29 1 2 78 52 117 6.01e-20 77.4
MsG0380013213.01.T01 MTR_3g067430 46.753 77 30 1 2 78 73 138 7.08e-20 77.8
MsG0380013213.01.T01 MTR_7g056803 55.738 61 26 1 5 64 74 134 7.12e-20 77.8
MsG0380013213.01.T01 MTR_7g056817 52.308 65 30 1 1 64 71 135 8.13e-20 77.8
MsG0380013213.01.T01 MTR_3g067270 50.000 68 33 1 2 68 67 134 1.33e-19 77.0
MsG0380013213.01.T01 MTR_3g467750 50.000 68 33 1 2 68 67 134 1.33e-19 77.0
MsG0380013213.01.T01 MTR_3g067280 50.000 68 33 1 2 68 67 134 1.51e-19 77.0
MsG0380013213.01.T01 MTR_3g067570 67.391 46 15 0 2 47 45 90 1.71e-19 75.5
MsG0380013213.01.T01 MTR_3g463570 46.250 80 31 2 2 80 68 136 1.76e-19 76.6
MsG0380013213.01.T01 MTR_3g067535 51.948 77 24 2 2 78 71 134 5.69e-19 75.5
MsG0380013213.01.T01 MTR_3g463570 46.250 80 31 2 2 80 88 156 6.56e-19 75.9
MsG0380013213.01.T01 MTR_4g026590 46.341 82 33 1 1 82 75 145 7.44e-19 75.5
MsG0380013213.01.T01 MTR_6g036620 48.718 78 29 2 1 78 66 132 1.93e-18 73.9
MsG0380013213.01.T01 MTR_3g067510 65.217 46 16 0 2 47 75 120 3.71e-18 73.2
MsG0380013213.01.T01 MTR_3g067510 65.217 46 16 0 2 47 70 115 4.09e-18 73.2
MsG0380013213.01.T01 MTR_6g047900 45.122 82 32 3 1 81 65 134 4.12e-18 73.2
MsG0380013213.01.T01 MTR_8g461020 46.575 73 28 2 1 72 67 129 2.64e-17 71.2
MsG0380013213.01.T01 MTR_6g047880 44.444 81 33 2 1 81 69 137 4.30e-17 70.5
MsG0380013213.01.T01 MTR_1g019650 49.020 51 26 0 5 55 60 110 9.72e-15 63.9
MsG0380013213.01.T01 MTR_3g067445 54.545 44 20 0 2 45 73 116 9.82e-15 63.9
MsG0380013213.01.T01 MTR_3g436120 77.500 40 9 0 43 82 62 101 2.15e-14 62.8
MsG0380013213.01.T01 MTR_7g029540 40.385 52 31 0 1 52 69 120 1.82e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATGCTGATATTGAATA+TGG 0.285356 3:+33014191 MsG0380013213.01.T01:CDS
AGACTACTTGAAGATGGATT+TGG 0.327144 3:+33014316 MsG0380013213.01.T01:CDS
GTAGAACGTTACTTCAAGAT+TGG 0.390047 3:+33014239 MsG0380013213.01.T01:CDS
CCATATTCAATATCAGCATT+AGG 0.437256 3:-33014191 None:intergenic
TGAGAAATATCATTCTTAAG+TGG 0.452118 3:-33014345 None:intergenic
GAGTGCATCAAGAAGGGAAG+TGG 0.522512 3:+33014149 MsG0380013213.01.T01:CDS
TCATGCCGAGTGCATCAAGA+AGG 0.559241 3:+33014142 MsG0380013213.01.T01:CDS
CGTTCTACTTCATTTACAGA+GGG 0.574809 3:-33014224 None:intergenic
ACTTCCCTTCTTGATGCACT+CGG 0.605609 3:-33014147 None:intergenic
CATGCCGAGTGCATCAAGAA+GGG 0.625240 3:+33014143 MsG0380013213.01.T01:CDS
ACGTTCTACTTCATTTACAG+AGG 0.638520 3:-33014225 None:intergenic
ATGCTGATATTGAATATGGA+TGG 0.639133 3:+33014195 MsG0380013213.01.T01:CDS
AGTGCATCAAGAAGGGAAGT+GGG 0.648471 3:+33014150 MsG0380013213.01.T01:CDS
ACATAATATGAAGATGATCG+AGG 0.692790 3:+33014097 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAGAAATTTTTTACTACA+GGG - Chr3:33014271-33014290 None:intergenic 20.0%
!!! TTGAAGAAATTTTTTACTAC+AGG - Chr3:33014272-33014291 None:intergenic 20.0%
ATGCTGATATTGAATATGGA+TGG + Chr3:33014195-33014214 MsG0380013213.01.T01:CDS 30.0%
CCATATTCAATATCAGCATT+AGG - Chr3:33014194-33014213 None:intergenic 30.0%
CCTAATGCTGATATTGAATA+TGG + Chr3:33014191-33014210 MsG0380013213.01.T01:CDS 30.0%
TGAAAAAGACTACTTGAAGA+TGG + Chr3:33014310-33014329 MsG0380013213.01.T01:CDS 30.0%
!! GTCTTTTTCATTAGAGTTTG+AGG - Chr3:33014300-33014319 None:intergenic 30.0%
!!! GAAATTTTTTACTACAGGGT+TGG - Chr3:33014267-33014286 None:intergenic 30.0%
ACGTTCTACTTCATTTACAG+AGG - Chr3:33014228-33014247 None:intergenic 35.0%
CGTTCTACTTCATTTACAGA+GGG - Chr3:33014227-33014246 None:intergenic 35.0%
GTAGAACGTTACTTCAAGAT+TGG + Chr3:33014239-33014258 MsG0380013213.01.T01:CDS 35.0%
! AGACTACTTGAAGATGGATT+TGG + Chr3:33014316-33014335 MsG0380013213.01.T01:CDS 35.0%
! GCATGAGTTTTGCATAAGTT+AGG - Chr3:33014128-33014147 None:intergenic 35.0%
ACTTCCCTTCTTGATGCACT+CGG - Chr3:33014150-33014169 None:intergenic 45.0%
AGTGCATCAAGAAGGGAAGT+GGG + Chr3:33014150-33014169 MsG0380013213.01.T01:CDS 45.0%
CATGCCGAGTGCATCAAGAA+GGG + Chr3:33014143-33014162 MsG0380013213.01.T01:CDS 50.0%
GAGTGCATCAAGAAGGGAAG+TGG + Chr3:33014149-33014168 MsG0380013213.01.T01:CDS 50.0%
TCATGCCGAGTGCATCAAGA+AGG + Chr3:33014142-33014161 MsG0380013213.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 33014104 33014352 33014104 ID=MsG0380013213.01;Name=MsG0380013213.01
Chr3 mRNA 33014104 33014352 33014104 ID=MsG0380013213.01.T01;Parent=MsG0380013213.01;Name=MsG0380013213.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|82
Chr3 exon 33014104 33014352 33014104 ID=MsG0380013213.01.T01:exon:41791;Parent=MsG0380013213.01.T01
Chr3 CDS 33014104 33014352 33014104 ID=MsG0380013213.01.T01:cds;Parent=MsG0380013213.01.T01
Gene Sequence

>MsG0380013213.01.T01

ATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCCGAGTGCATCAAGAAGGGAAGTGGGAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTGTTTTCCCTCTGTAAATGAAGTAGAACGTTACTTCAAGATTGGATCCAACCCTGTAGTAAAAAATTTCTTCAAGATTGCCTCAAACTCTAATGAAAAAGACTACTTGAAGATGGATTTGGAAATTGCCACTTAA

Protein sequence

>MsG0380013213.01.T01

MKMIEEHPNLCKTHAECIKKGSGNFCARYPNADIEYGWCFPSVNEVERYFKIGSNPVVKNFFKIASNSNEKDYLKMDLEIAT*