AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017998.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017998.01.T01 AT4G39260 84.375 32 5 0 1 32 60 91 1.19e-11 57.0

Find 72 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGATATGGTGGAGGAGG+CGG 0.278119 3:+103052187 MsG0380017998.01.T01:CDS
CGTGATGGTGGTGGTGGATA+TGG 0.293140 3:+103052238 MsG0380017998.01.T01:CDS
GTGGATATGGTGGAGGAGGC+GGG 0.301740 3:+103052188 MsG0380017998.01.T01:CDS
CGCGGAGGTGGTGGTGGATA+TGG 0.305180 3:+103052085 MsG0380017998.01.T01:CDS
CGTGGAGGAGGTGGTGGATA+TGG 0.306969 3:+103052175 MsG0380017998.01.T01:CDS
CGTCGTGGAGGTGGTGGATA+TGG 0.314693 3:+103052121 MsG0380017998.01.T01:CDS
TGGTGGATATGGTGGAGGGG+GGG 0.315756 3:+103052132 MsG0380017998.01.T01:CDS
GTGGTGGATATGGTGGAGGG+GGG 0.319876 3:+103052131 MsG0380017998.01.T01:CDS
AGTGGCGGAGGCGGTGGTGG+TGG 0.340361 3:+103052055 MsG0380017998.01.T01:CDS
GGTGGTGGATATGGTGGAGG+GGG 0.362455 3:+103052130 MsG0380017998.01.T01:CDS
TGGATATGGTGGAGGAGGCG+GGG 0.372204 3:+103052189 MsG0380017998.01.T01:CDS
ATCACCGCCTCTCGAGTATC+TGG 0.373006 3:-103052287 None:intergenic
GGCGGAGGCGGTGGTGGTGG+TGG 0.379371 3:+103052058 MsG0380017998.01.T01:CDS
GGCAGTGGCGGAGGCGGTGG+TGG 0.383736 3:+103052052 MsG0380017998.01.T01:CDS
GGAGGTGGTGGATATGGTGG+AGG 0.387649 3:+103052127 MsG0380017998.01.T01:CDS
GGATATGGTGGAGGAGGCGG+GGG 0.387784 3:+103052190 MsG0380017998.01.T01:CDS
CGTGGCAGTGGCGGAGGCGG+TGG 0.400483 3:+103052049 MsG0380017998.01.T01:CDS
GGATACCGCGGAGGTGGTGG+TGG 0.412147 3:+103052079 MsG0380017998.01.T01:CDS
GGTGGTGGATATGGCGGAGG+TGG 0.417575 3:+103052094 MsG0380017998.01.T01:CDS
GGTGGATACCGCGGAGGTGG+TGG 0.418043 3:+103052076 MsG0380017998.01.T01:CDS
GGTTACGGAGGTGGTGGTGG+CGG 0.440050 3:+103052211 MsG0380017998.01.T01:CDS
GGTGGTGACCGTCGTGGAGG+AGG 0.444501 3:+103052163 MsG0380017998.01.T01:CDS
GACCGTCGTGGAGGAGGTGG+TGG 0.446949 3:+103052169 MsG0380017998.01.T01:CDS
GGTGGTGGATATGGTGGAGG+AGG 0.448331 3:+103052184 MsG0380017998.01.T01:CDS
AGGTGGTGGATATGGTGGAG+GGG 0.449196 3:+103052129 MsG0380017998.01.T01:CDS
GGTGGTGAACGTCGTGGAGG+TGG 0.450443 3:+103052112 MsG0380017998.01.T01:CDS
CAGAGTCGTGGCAGTGGCGG+AGG 0.453358 3:+103052043 MsG0380017998.01.T01:CDS
GGTGGTGGATACCGCGGAGG+TGG 0.455995 3:+103052073 MsG0380017998.01.T01:CDS
AGTCGTGGCAGTGGCGGAGG+CGG 0.456959 3:+103052046 MsG0380017998.01.T01:CDS
GGTGACCGTCGTGGAGGAGG+TGG 0.465249 3:+103052166 MsG0380017998.01.T01:CDS
GATGGTGGTGGTGGATATGG+TGG 0.469678 3:+103052241 MsG0380017998.01.T01:CDS
GGTGGTGGATATGGTGGAGG+TGG 0.471808 3:+103052247 MsG0380017998.01.T01:CDS
GGATATGGTGGAGGTGGCTA+CGG 0.473053 3:+103052253 MsG0380017998.01.T01:CDS
GGAGGAGGTGGTGGATATGG+TGG 0.473329 3:+103052178 MsG0380017998.01.T01:CDS
GGAGGTGGTGGATATGGTGG+AGG 0.473487 3:+103052181 MsG0380017998.01.T01:CDS
GGTGGTGGTGGATATGGCGG+AGG 0.496613 3:+103052091 MsG0380017998.01.T01:CDS
GGTGGTGGTGGATATGGTGG+AGG 0.497470 3:+103052244 MsG0380017998.01.T01:CDS
GCGATGAAAGATGCGATTGA+AGG 0.503882 3:+103051971 None:intergenic
ATGAACGGCCAAGATCTTGA+TGG 0.505893 3:+103051995 MsG0380017998.01.T01:CDS
GGTGGAGGTGGCTACGGAGA+TGG 0.509409 3:+103052259 MsG0380017998.01.T01:CDS
GGACGTCGTGATGGTGGTGG+TGG 0.511175 3:+103052232 MsG0380017998.01.T01:CDS
CGTGGAGGTGGTGGATATGG+TGG 0.512534 3:+103052124 MsG0380017998.01.T01:CDS
GGTGGTGGCGGACGTCGTGA+TGG 0.514583 3:+103052223 MsG0380017998.01.T01:CDS
GGTGAACGTCGTGGAGGTGG+TGG 0.516929 3:+103052115 MsG0380017998.01.T01:CDS
GAGGCTCAGAGTCGTGGCAG+TGG 0.522443 3:+103052037 MsG0380017998.01.T01:CDS
TGTTACGTCCATCAAGATCT+TGG 0.527371 3:-103052003 None:intergenic
TCGAGAGGCGGTGATGGCGG+TGG 0.528930 3:+103052295 MsG0380017998.01.T01:CDS
GAGGTGGTGGATATGGTGGA+GGG 0.530422 3:+103052128 MsG0380017998.01.T01:CDS
GGCGGACGTCGTGATGGTGG+TGG 0.542567 3:+103052229 MsG0380017998.01.T01:CDS
CGATGAAAGATGCGATTGAA+GGG 0.542649 3:+103051972 None:intergenic
GCTCAGAGTCGTGGCAGTGG+CGG 0.545233 3:+103052040 MsG0380017998.01.T01:CDS
GGTGGCGGACGTCGTGATGG+TGG 0.554420 3:+103052226 MsG0380017998.01.T01:CDS
AGATACTCGAGAGGCGGTGA+TGG 0.555850 3:+103052289 MsG0380017998.01.T01:CDS
GGAGGTGGCTACGGAGATGG+TGG 0.556120 3:+103052262 MsG0380017998.01.T01:CDS
GGTGGCTCTGACAGTAGAAA+CGG 0.574486 3:+103052313 MsG0380017998.01.T01:CDS
TACTCGAGAGGCGGTGATGG+CGG 0.579779 3:+103052292 MsG0380017998.01.T01:CDS
ACGTAACATCACTGTGAATG+AGG 0.581570 3:+103052018 MsG0380017998.01.T01:CDS
GGAGGTGGTGGTGGATATGG+CGG 0.597374 3:+103052088 MsG0380017998.01.T01:CDS
TATGGAGGTGGTGACCGTCG+TGG 0.615014 3:+103052157 MsG0380017998.01.T01:CDS
GATGCGATTGAAGGGATGAA+CGG 0.619661 3:+103051980 MsG0380017998.01.T01:CDS
GTGAATGAGGCTCAGAGTCG+TGG 0.619893 3:+103052031 MsG0380017998.01.T01:CDS
GGTGGTGGTGGTGGATACCG+CGG 0.621729 3:+103052067 MsG0380017998.01.T01:CDS
GGCTCCAGATACTCGAGAGG+CGG 0.636811 3:+103052283 MsG0380017998.01.T01:CDS
GGAGGTGGTGAACGTCGTGG+AGG 0.637604 3:+103052109 MsG0380017998.01.T01:CDS
GGCTCTGACAGTAGAAACGG+AGG 0.641554 3:+103052316 MsG0380017998.01.T01:CDS
GGAGGTGGTGACCGTCGTGG+AGG 0.653524 3:+103052160 MsG0380017998.01.T01:CDS
GGCGGAGGTGGTGAACGTCG+TGG 0.655000 3:+103052106 MsG0380017998.01.T01:CDS
GGTGGTGGTGGATACCGCGG+AGG 0.661432 3:+103052070 MsG0380017998.01.T01:CDS
ATCCACCACCTCCTCCACGA+CGG 0.697052 3:-103052171 None:intergenic
CATATCCACCACCACCTCCG+CGG 0.713479 3:-103052084 None:intergenic
GGTGGCTCCAGATACTCGAG+AGG 0.728248 3:+103052280 MsG0380017998.01.T01:CDS
GCTCTGACAGTAGAAACGGA+GGG 0.729657 3:+103052317 MsG0380017998.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACGTAACATCACTGTGAATG+AGG + Chr3:103052018-103052037 MsG0380017998.01.T01:CDS 40.0%
TGTTACGTCCATCAAGATCT+TGG - Chr3:103052006-103052025 None:intergenic 40.0%
ATGAACGGCCAAGATCTTGA+TGG + Chr3:103051995-103052014 MsG0380017998.01.T01:CDS 45.0%
GATGCGATTGAAGGGATGAA+CGG + Chr3:103051980-103051999 MsG0380017998.01.T01:CDS 45.0%
GCTCTGACAGTAGAAACGGA+GGG + Chr3:103052317-103052336 MsG0380017998.01.T01:CDS 50.0%
!! GGTGGCTCTGACAGTAGAAA+CGG + Chr3:103052313-103052332 MsG0380017998.01.T01:CDS 50.0%
AGATACTCGAGAGGCGGTGA+TGG + Chr3:103052289-103052308 MsG0380017998.01.T01:CDS 55.0%
ATCACCGCCTCTCGAGTATC+TGG - Chr3:103052290-103052309 None:intergenic 55.0%
GTGAATGAGGCTCAGAGTCG+TGG + Chr3:103052031-103052050 MsG0380017998.01.T01:CDS 55.0%
! GGATATGGTGGAGGTGGCTA+CGG + Chr3:103052253-103052272 MsG0380017998.01.T01:CDS 55.0%
! GGCTCTGACAGTAGAAACGG+AGG + Chr3:103052316-103052335 MsG0380017998.01.T01:CDS 55.0%
!! AGGTGGTGGATATGGTGGAG+GGG + Chr3:103052129-103052148 MsG0380017998.01.T01:CDS 55.0%
!! CGTGATGGTGGTGGTGGATA+TGG + Chr3:103052238-103052257 MsG0380017998.01.T01:CDS 55.0%
!! GAGGTGGTGGATATGGTGGA+GGG + Chr3:103052128-103052147 MsG0380017998.01.T01:CDS 55.0%
!! GATGGTGGTGGTGGATATGG+TGG + Chr3:103052241-103052260 MsG0380017998.01.T01:CDS 55.0%
ATCCACCACCTCCTCCACGA+CGG - Chr3:103052174-103052193 None:intergenic 60.0%
CATATCCACCACCACCTCCG+CGG - Chr3:103052087-103052106 None:intergenic 60.0%
GGCTCCAGATACTCGAGAGG+CGG + Chr3:103052283-103052302 MsG0380017998.01.T01:CDS 60.0%
GGTGGCTCCAGATACTCGAG+AGG + Chr3:103052280-103052299 MsG0380017998.01.T01:CDS 60.0%
TATGGAGGTGGTGACCGTCG+TGG + Chr3:103052157-103052176 MsG0380017998.01.T01:CDS 60.0%
! GGTGGATATGGTGGAGGAGG+CGG + Chr3:103052187-103052206 MsG0380017998.01.T01:CDS 60.0%
! GTGGATATGGTGGAGGAGGC+GGG + Chr3:103052188-103052207 MsG0380017998.01.T01:CDS 60.0%
! TACTCGAGAGGCGGTGATGG+CGG + Chr3:103052292-103052311 MsG0380017998.01.T01:CDS 60.0%
! TATGGTGGAGGGGGGGGTTA+TGG + Chr3:103052139-103052158 MsG0380017998.01.T01:CDS 60.0%
! TGGATATGGTGGAGGAGGCG+GGG + Chr3:103052189-103052208 MsG0380017998.01.T01:CDS 60.0%
! TGGTGGATATGGTGGAGGGG+GGG + Chr3:103052132-103052151 MsG0380017998.01.T01:CDS 60.0%
!! CGTCGTGGAGGTGGTGGATA+TGG + Chr3:103052121-103052140 MsG0380017998.01.T01:CDS 60.0%
!! CGTGGAGGAGGTGGTGGATA+TGG + Chr3:103052175-103052194 MsG0380017998.01.T01:CDS 60.0%
!! CGTGGAGGTGGTGGATATGG+TGG + Chr3:103052124-103052143 MsG0380017998.01.T01:CDS 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG + Chr3:103052178-103052197 MsG0380017998.01.T01:CDS 60.0%
!! GGAGGTGGTGGATATGGTGG+AGG + Chr3:103052127-103052146 MsG0380017998.01.T01:CDS 60.0%
!! GGAGGTGGTGGTGGATATGG+CGG + Chr3:103052088-103052107 MsG0380017998.01.T01:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG + Chr3:103052130-103052149 MsG0380017998.01.T01:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+GGG + Chr3:103052130-103052149 MsG0380017998.01.T01:CDS 60.0%
!! GGTGGTGGATATGGTGGAGG+TGG + Chr3:103052130-103052149 MsG0380017998.01.T01:CDS 60.0%
!! GGTGGTGGTGGATATGGTGG+AGG + Chr3:103052244-103052263 MsG0380017998.01.T01:CDS 60.0%
!! GTGGTGGATATGGTGGAGGG+GGG + Chr3:103052131-103052150 MsG0380017998.01.T01:CDS 60.0%
GAGGCTCAGAGTCGTGGCAG+TGG + Chr3:103052037-103052056 MsG0380017998.01.T01:CDS 65.0%
GCTCAGAGTCGTGGCAGTGG+CGG + Chr3:103052040-103052059 MsG0380017998.01.T01:CDS 65.0%
GGAGGTGGCTACGGAGATGG+TGG + Chr3:103052262-103052281 MsG0380017998.01.T01:CDS 65.0%
GGAGGTGGTGAACGTCGTGG+AGG + Chr3:103052109-103052128 MsG0380017998.01.T01:CDS 65.0%
GGTGAACGTCGTGGAGGTGG+TGG + Chr3:103052115-103052134 MsG0380017998.01.T01:CDS 65.0%
GGTGGAGGTGGCTACGGAGA+TGG + Chr3:103052259-103052278 MsG0380017998.01.T01:CDS 65.0%
GGTGGTGAACGTCGTGGAGG+TGG + Chr3:103052112-103052131 MsG0380017998.01.T01:CDS 65.0%
! GGATATGGTGGAGGAGGCGG+GGG + Chr3:103052190-103052209 MsG0380017998.01.T01:CDS 65.0%
! GGTGGATATGGTGGAGGGGG+GGG + Chr3:103052133-103052152 MsG0380017998.01.T01:CDS 65.0%
!! CGCGGAGGTGGTGGTGGATA+TGG + Chr3:103052085-103052104 MsG0380017998.01.T01:CDS 65.0%
!! GGACGTCGTGATGGTGGTGG+TGG + Chr3:103052232-103052251 MsG0380017998.01.T01:CDS 65.0%
!! GGTGGTGGATATGGCGGAGG+TGG + Chr3:103052094-103052113 MsG0380017998.01.T01:CDS 65.0%
!! GGTGGTGGTGGATATGGCGG+AGG + Chr3:103052091-103052110 MsG0380017998.01.T01:CDS 65.0%
!! GGTGGTGGTGGTGGATACCG+CGG + Chr3:103052067-103052086 MsG0380017998.01.T01:CDS 65.0%
!! GGTTACGGAGGTGGTGGTGG+CGG + Chr3:103052211-103052230 MsG0380017998.01.T01:CDS 65.0%
CAGAGTCGTGGCAGTGGCGG+AGG + Chr3:103052043-103052062 MsG0380017998.01.T01:CDS 70.0%
GACCGTCGTGGAGGAGGTGG+TGG + Chr3:103052169-103052188 MsG0380017998.01.T01:CDS 70.0%
GGAGGGGGGGGTTATGGAGG+TGG + Chr3:103052145-103052164 MsG0380017998.01.T01:CDS 70.0%
GGAGGTGGTGACCGTCGTGG+AGG + Chr3:103052160-103052179 MsG0380017998.01.T01:CDS 70.0%
GGCGGAGGTGGTGAACGTCG+TGG + Chr3:103052106-103052125 MsG0380017998.01.T01:CDS 70.0%
GGTGACCGTCGTGGAGGAGG+TGG + Chr3:103052166-103052185 MsG0380017998.01.T01:CDS 70.0%
GGTGGAGGAGGCGGGGGTTA+CGG + Chr3:103052196-103052215 MsG0380017998.01.T01:CDS 70.0%
GGTGGAGGGGGGGGTTATGG+AGG + Chr3:103052142-103052161 MsG0380017998.01.T01:CDS 70.0%
GGTGGATACCGCGGAGGTGG+TGG + Chr3:103052076-103052095 MsG0380017998.01.T01:CDS 70.0%
GGTGGTGACCGTCGTGGAGG+AGG + Chr3:103052163-103052182 MsG0380017998.01.T01:CDS 70.0%
! AGTCGTGGCAGTGGCGGAGG+CGG + Chr3:103052046-103052065 MsG0380017998.01.T01:CDS 70.0%
! GGATACCGCGGAGGTGGTGG+TGG + Chr3:103052079-103052098 MsG0380017998.01.T01:CDS 70.0%
! GGGGGTTACGGAGGTGGTGG+TGG + Chr3:103052208-103052227 MsG0380017998.01.T01:CDS 70.0%
!! GGCGGACGTCGTGATGGTGG+TGG + Chr3:103052229-103052248 MsG0380017998.01.T01:CDS 70.0%
!! GGTGGCGGACGTCGTGATGG+TGG + Chr3:103052226-103052245 MsG0380017998.01.T01:CDS 70.0%
!! GGTGGTGGATACCGCGGAGG+TGG + Chr3:103052073-103052092 MsG0380017998.01.T01:CDS 70.0%
!! GGTGGTGGCGGACGTCGTGA+TGG + Chr3:103052223-103052242 MsG0380017998.01.T01:CDS 70.0%
!! GGTGGTGGTGGATACCGCGG+AGG + Chr3:103052070-103052089 MsG0380017998.01.T01:CDS 70.0%
!! TCGAGAGGCGGTGATGGCGG+TGG + Chr3:103052295-103052314 MsG0380017998.01.T01:CDS 70.0%
GGAGGAGGCGGGGGTTACGG+AGG + Chr3:103052199-103052218 MsG0380017998.01.T01:CDS 75.0%
GGAGGCGGGGGTTACGGAGG+TGG + Chr3:103052202-103052221 MsG0380017998.01.T01:CDS 75.0%
GGCGGGGGTTACGGAGGTGG+TGG + Chr3:103052205-103052224 MsG0380017998.01.T01:CDS 75.0%
! AGTGGCGGAGGCGGTGGTGG+TGG + Chr3:103052055-103052074 MsG0380017998.01.T01:CDS 75.0%
!! CGTGGCAGTGGCGGAGGCGG+TGG + Chr3:103052049-103052068 MsG0380017998.01.T01:CDS 80.0%
!! GGCAGTGGCGGAGGCGGTGG+TGG + Chr3:103052052-103052071 MsG0380017998.01.T01:CDS 80.0%
!!! GGCGGAGGCGGTGGTGGTGG+TGG + Chr3:103052058-103052077 MsG0380017998.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 103051974 103052354 103051974 ID=MsG0380017998.01;Name=MsG0380017998.01
Chr3 mRNA 103051974 103052354 103051974 ID=MsG0380017998.01.T01;Parent=MsG0380017998.01;Name=MsG0380017998.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|126
Chr3 exon 103051974 103052354 103051974 ID=MsG0380017998.01.T01:exon:3107;Parent=MsG0380017998.01.T01
Chr3 CDS 103051974 103052354 103051974 ID=MsG0380017998.01.T01:cds;Parent=MsG0380017998.01.T01
Gene Sequence

>MsG0380017998.01.T01

ATGAAAGATGCGATTGAAGGGATGAACGGCCAAGATCTTGATGGACGTAACATCACTGTGAATGAGGCTCAGAGTCGTGGCAGTGGCGGAGGCGGTGGTGGTGGTGGATACCGCGGAGGTGGTGGTGGATATGGCGGAGGTGGTGAACGTCGTGGAGGTGGTGGATATGGTGGAGGGGGGGGTTATGGAGGTGGTGACCGTCGTGGAGGAGGTGGTGGATATGGTGGAGGAGGCGGGGGTTACGGAGGTGGTGGTGGCGGACGTCGTGATGGTGGTGGTGGATATGGTGGAGGTGGCTACGGAGATGGTGGCTCCAGATACTCGAGAGGCGGTGATGGCGGTGGCTCTGACAGTAGAAACGGAGGGAATAAGTTTCGTTGA

Protein sequence

>MsG0380017998.01.T01

MKDAIEGMNGQDLDGRNITVNEAQSRGSGGGGGGGGYRGGGGGYGGGGERRGGGGYGGGGGYGGGDRRGGGGGYGGGGGGYGGGGGGRRDGGGGYGGGGYGDGGSRYSRGGDGGGSDSRNGGNKFR*