AlfalfaGEDB | MsG0880044003.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044003.01

KEGG: K02868
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00281 PF00673

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044003.01.T01	XP_003628237.1	97.79	181	4	0	112	292	1	181	4.48E-125	364

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044003.01.T01	P46287	97.79	181	4	0	112	292	1	181	7.09E-128	364

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048922.01	MsG0880044003.01	0.810120	1.349784e-50	8.068878e-48
MsG0180002738.01	MsG0880044003.01	0.800209	1.604186e-48	7.426225e-46
MsG0180002810.01	MsG0880044003.01	0.813725	2.212884e-51	1.456446e-48
MsG0180003115.01	MsG0880044003.01	0.803624	3.190007e-49	1.610725e-46
MsG0180005573.01	MsG0880044003.01	0.846579	1.987943e-59	3.437311e-56
MsG0280007643.01	MsG0880044003.01	0.815536	8.789895e-52	6.076680e-49
MsG0280010495.01	MsG0880044003.01	0.810544	1.093508e-50	6.609932e-48
MsG0280011060.01	MsG0880044003.01	0.825424	4.723951e-54	4.303844e-51
MsG0380015494.01	MsG0880044003.01	0.844001	9.902590e-59	1.577062e-55
MsG0380016577.01	MsG0880044003.01	0.823759	1.164833e-53	1.012235e-50

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PPI

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Gene1	Gene2	Type
MsG0580024916.01	MsG0880044003.01	PPI
MsG0880044003.01	MsG0180003409.01	PPI
MsG0880044003.01	MsG0780039623.01	PPI
MsG0880044003.01	MsG0280009999.01	PPI
MsG0880044003.01	MsG0480022006.01	PPI
MsG0880044003.01	MsG0880047038.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044003.01.T01	MTR_8g046140	97.790	181	4	0	112	292	1	181	5.42e-129	364
MsG0880044003.01.T01	MTR_3g007700	98.333	180	3	0	113	292	3	182	6.00e-129	364
MsG0880044003.01.T01	MTR_4g080740	96.685	181	6	0	112	292	1	181	5.29e-128	362
MsG0880044003.01.T01	MTR_3g007690	80.337	178	34	1	115	291	64	241	6.07e-99	290
MsG0880044003.01.T01	MTR_7g033205	73.504	117	30	1	2	117	26	142	2.78e-56	179
MsG0880044003.01.T01	MTR_2g061390	71.186	118	32	1	2	117	35	152	4.60e-56	179
MsG0880044003.01.T01	MTR_8g027132	69.159	107	31	2	21	126	5	110	1.19e-44	149
MsG0880044003.01.T01	MTR_4g098825	55.172	116	27	1	2	117	10	100	2.63e-35	123
MsG0880044003.01.T01	MTR_3g030100	70.886	79	22	1	33	110	12	90	2.78e-32	115
MsG0880044003.01.T01	MTR_5g064800	74.390	82	20	1	38	118	292	373	3.66e-29	115

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044003.01.T01	AT2G42740	95.028	181	9	112	292	1	181	1.40e-125	356
MsG0880044003.01.T01	AT5G45775	94.475	181	10	112	292	1	181	6.65e-125	354
MsG0880044003.01.T01	AT4G18730	94.475	181	10	112	292	1	181	6.65e-125	354
MsG0880044003.01.T01	AT3G58700	94.475	181	10	112	292	1	181	6.65e-125	354
MsG0880044003.01.T01	AT5G45775	95.906	171	7	122	292	1	171	1.11e-119	340

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Find 71 sgRNAs with CRISPR-Local

Find 333 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCTTAATGTTGATGCAGC+AGG	0.452096	8:-34211452	MsG0880044003.01.T01:CDS
AAGTAACTAAACAATCGCCT+GGG	0.633971	8:+34206291	None:intergenic
AATAAACCTTTGGAAAACAC+TGG	0.500470	8:+34209188	None:intergenic
AATACCGAATCCAAAACACC+CGG	0.688290	8:+34207049	None:intergenic
AATATCTCTGTTGGTGAAAG+TGG	0.486734	8:-34209348	MsG0880044003.01.T01:CDS
AATCCAACATCGCGTAACAA+AGG	0.595227	8:-34206368	MsG0880044003.01.T01:CDS
ACACCATATTTCATAACACA+TGG	0.684276	8:+34211652	None:intergenic
ACATCAGATATTGGCAAAAT+TGG	0.206917	8:+34211503	None:intergenic
ACCGACGACGACGTCCCACA+CGG	0.654712	8:+34206407	None:intergenic
ACCGTGTGGGACGTCGTCGT+CGG	0.567737	8:-34206408	MsG0880044003.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTTGATTTAGCATGCA+GGG	+	Chr8:34209950-34209969	None:intergenic	30.0%
AAATTATGGCTAAAGCTATC+AGG	-	Chr8:34210991-34211010	MsG0880044003.01.T01:intron	30.0%
AACAACAACAACAACAACAA+AGG	+	Chr8:34208322-34208341	None:intergenic	30.0%
AACAGTTTCAACTCAAAACA+TGG	+	Chr8:34209910-34209929	None:intergenic	30.0%
AAGAGGCGATTAAATATGTT+TGG	+	Chr8:34206818-34206837	None:intergenic	30.0%
ACACCATATTTCATAACACA+TGG	+	Chr8:34206134-34206153	None:intergenic	30.0%
ACAGTTTCAACTCAAAACAT+GGG	+	Chr8:34209909-34209928	None:intergenic	30.0%
ACATCAGATATTGGCAAAAT+TGG	+	Chr8:34206283-34206302	None:intergenic	30.0%
ACTATTCTATTTGATACTGG+GGG	-	Chr8:34209982-34210001	MsG0880044003.01.T01:intron	30.0%
AGAGGCGATTAAATATGTTT+GGG	+	Chr8:34206817-34206836	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	34206055	34211750	34206055	ID=MsG0880044003.01;Name=MsG0880044003.01
Chr8	mRNA	34206055	34211750	34206055	ID=MsG0880044003.01.T01;Parent=MsG0880044003.01;Name=MsG0880044003.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|0.8\|0.83\|1\|0.8\|0.5\|6\|189\|292
Chr8	exon	34206055	34206179	34206055	ID=MsG0880044003.01.T01:exon:8178;Parent=MsG0880044003.01.T01
Chr8	exon	34206233	34206492	34206233	ID=MsG0880044003.01.T01:exon:8177;Parent=MsG0880044003.01.T01
Chr8	exon	34207023	34207218	34207023	ID=MsG0880044003.01.T01:exon:8176;Parent=MsG0880044003.01.T01
Chr8	exon	34209195	34209237	34209195	ID=MsG0880044003.01.T01:exon:8175;Parent=MsG0880044003.01.T01
Chr8	exon	34209323	34209430	34209323	ID=MsG0880044003.01.T01:exon:8174;Parent=MsG0880044003.01.T01
Chr8	exon	34211415	34211750	34211415	ID=MsG0880044003.01.T01:exon:8173;Parent=MsG0880044003.01.T01
Chr8	CDS	34211415	34211750	34211415	ID=MsG0880044003.01.T01:cds;Parent=MsG0880044003.01.T01
Chr8	CDS	34209323	34209430	34209323	ID=MsG0880044003.01.T01:cds;Parent=MsG0880044003.01.T01
Chr8	CDS	34209195	34209237	34209195	ID=MsG0880044003.01.T01:cds;Parent=MsG0880044003.01.T01
Chr8	CDS	34207023	34207218	34207023	ID=MsG0880044003.01.T01:cds;Parent=MsG0880044003.01.T01
Chr8	CDS	34206297	34206492	34206297	ID=MsG0880044003.01.T01:cds;Parent=MsG0880044003.01.T01
Chr8	three_prime_UTR	34206233	34206296	34206233	ID=MsG0880044003.01.T01:three_prime_utr;Parent=MsG0880044003.01.T01
Chr8	three_prime_UTR	34206055	34206179	34206055	ID=MsG0880044003.01.T01:three_prime_utr;Parent=MsG0880044003.01.T01

Gene Sequence

>MsG0880044003.01.T01

ATGTACATACCTTTTTCACAGTGGTTGGAGAGAAATATTATTGATGCTGATGGAGATATTATAGTTCATGTTTTGGCCATGTGTTATGAAATATGGTGTGCTAGAAATAAGAAATATTTTGAGCAGACTGATGTTGATGTGACTGCCATTGTTCAAAAGGCACAAAGATCTATTGTGAATTTTAAGAGTGTTAATACGATGTTGTTGGAGGCACTAAGTGAAAGTCCAATTTTGCCAATATCTGATGTTCATTGGACACCTTTTAGTGGTTTCTATAAGCTTAATGTTGATGCAGCAGGTTCGACAGAGGAGGATAAGTGGGGTATTGGTGGTGTGGTTTCGGAGAAGAAACTCTCCAACACCATGAGGGATATCAAGGTTCAGAAACTCGTTCTTAATATCTCTGTTGGTGAAAGTGGCGATCGTCTCACCAGAGCTGCAAAGGTGTTGGAACAACTCAGTGGCCAAACCCCAGTGTTTTCCAAAGCTAGATACACCGTCCGTTCTTTTGGTATTAGAAGAAATGAAAAGATTGCTTGCTATGTGACTGTTAGAGGTGACAAGGCAATGCAGCTTTTGGAGAGTGGTTTGAAGGTGAAGGAATATGAATTGCTGAGAAGAAATTTTAGTGATACCGGGTGTTTTGGATTCGGTATTCAGGAGCATATTGATCTGGGAATCAAGTATGATCCTTCAACTGGCATTTATGGAATGGACTTTTTCGTTGTTCTAGAGCGTCCCGGTTACCGTGTGGGACGTCGTCGTCGGTGCAAGGCTCGTGTTGGAATCCAACATCGCGTAACAAAGGACGATGCTATGAAGTGGTTCCAAGTGAAATATGAAGGTGTGATCCTCAACAAGTCCCAGGCGATTGTTTAG

Protein sequence

>MsG0880044003.01.T01

MYIPFSQWLERNIIDADGDIIVHVLAMCYEIWCARNKKYFEQTDVDVTAIVQKAQRSIVNFKSVNTMLLEALSESPILPISDVHWTPFSGFYKLNVDAAGSTEEDKWGIGGVVSEKKLSNTMRDIKVQKLVLNISVGESGDRLTRAAKVLEQLSGQTPVFSKARYTVRSFGIRRNEKIACYVTVRGDKAMQLLESGLKVKEYELLRRNFSDTGCFGFGIQEHIDLGIKYDPSTGIYGMDFFVVLERPGYRVGRRRRCKARVGIQHRVTKDDAMKWFQVKYEGVILNKSQAIV*