AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030073.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030073.01.T01 MTR_3g094220 98.387 62 1 0 44 105 1 62 3.61e-41 133
MsG0580030073.01.T01 MTR_3g094220 96.825 63 2 0 44 106 1 63 4.93e-41 132
MsG0580030073.01.T01 MTR_5g097200 96.825 63 2 0 44 106 1 63 5.71e-41 132
MsG0580030073.01.T01 MTR_5g097200 98.387 62 1 0 44 105 1 62 5.77e-41 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030073.01.T01 AT3G56340 87.302 63 8 0 44 106 1 63 3.64e-36 120
MsG0580030073.01.T01 AT2G40590 85.714 63 9 0 44 106 1 63 2.86e-35 118
MsG0580030073.01.T01 AT2G40510 85.714 63 9 0 44 106 1 63 3.38e-35 118

Find 21 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGAAAGTGCAGGTTGTT+TGG 0.255247 5:-106242426 MsG0580030073.01.T01:CDS
CAATCAAGAGGTTTCTTGTT+AGG 0.291662 5:-106240859 MsG0580030073.01.T01:CDS
AGCTGCAGTTAGAGATGTTC+AGG 0.305494 5:-106240822 MsG0580030073.01.T01:CDS
ATTGAAAGTGCAGGTTGTTT+GGG 0.327340 5:-106242425 MsG0580030073.01.T01:CDS
AGTGCAGGTTGTTTGGGTTT+TGG 0.339642 5:-106242419 MsG0580030073.01.T01:CDS
TAGACATTTAAGCGCAGAAA+TGG 0.429840 5:-106241057 MsG0580030073.01.T01:CDS
AAACAACCTGCACTTTCAAT+AGG 0.434512 5:+106242428 None:intergenic
GGGACAGCATTTACCACAGT+TGG 0.475372 5:+106240987 None:intergenic
GGCCACGACCATGTTTGTTA+CGG 0.475572 5:+106241031 None:intergenic
ATTCTTCCTATTGAAAGTGC+AGG 0.495431 5:-106242434 MsG0580030073.01.T01:CDS
GTCAACTGTTGTTGTAATGT+TGG 0.508966 5:+106242376 None:intergenic
CATTACAACAACAGTTGACA+TGG 0.540817 5:-106242372 MsG0580030073.01.T01:intron
TTCATTCGTTGCTCCAACTG+TGG 0.541200 5:-106241000 MsG0580030073.01.T01:CDS
ACATTTAAGCGCAGAAATGG+AGG 0.573713 5:-106241054 MsG0580030073.01.T01:CDS
GGCCGTAACAAACATGGTCG+TGG 0.575945 5:-106241033 MsG0580030073.01.T01:CDS
TGAGTTAGATGAATCATCAG+AGG 0.607780 5:-106242459 MsG0580030073.01.T01:CDS
AATGGAGGCCGTAACAAACA+TGG 0.615814 5:-106241039 MsG0580030073.01.T01:CDS
AGATGCAAATCATTGATGGA+AGG 0.617311 5:-106242497 None:intergenic
CTGTGGTAAATGCTGTCCCA+AGG 0.625759 5:-106240983 MsG0580030073.01.T01:intron
AGCAACGAATGAATTTGACA+TGG 0.665067 5:+106241010 None:intergenic
AACAGGACAAGGCAATCAAG+AGG 0.667211 5:-106240871 MsG0580030073.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAAAACTATAACAAA+CGG + Chr5:106241138-106241157 None:intergenic 10.0%
!! TTTAAAAAAAAAAAGTGAAA+AGG - Chr5:106241829-106241848 MsG0580030073.01.T01:intron 10.0%
!! TTTATGAAAACTATATAAAA+GGG + Chr5:106241358-106241377 None:intergenic 10.0%
!!! AAAAAAAAATTGTATTTTGT+TGG + Chr5:106241565-106241584 None:intergenic 10.0%
!!! AAAATAAGTGATTTTTATTA+CGG - Chr5:106241450-106241469 MsG0580030073.01.T01:intron 10.0%
!!! AAAATACATTTTTTTTTTGA+AGG - Chr5:106241595-106241614 MsG0580030073.01.T01:intron 10.0%
!!! ACAAAATACAATTTTTTTTT+AGG - Chr5:106241565-106241584 MsG0580030073.01.T01:intron 10.0%
!!! ATATAATCTATTTTCAAATA+TGG + Chr5:106241787-106241806 None:intergenic 10.0%
!!! TATAATCTATTTTCAAATAT+GGG + Chr5:106241786-106241805 None:intergenic 10.0%
!!! TTTTATGAAAACTATATAAA+AGG + Chr5:106241359-106241378 None:intergenic 10.0%
!!! TTTTTAAAAGTTGTAATAAA+CGG + Chr5:106241210-106241229 None:intergenic 10.0%
!! AAAAAAACAGAAAAATCTTA+CGG + Chr5:106242362-106242381 None:intergenic 15.0%
!! ATAAAAAAACTATAACAAAC+GGG + Chr5:106241137-106241156 None:intergenic 15.0%
!!! TTATTTAAAATTTCCTCTTT+TGG + Chr5:106241697-106241716 None:intergenic 15.0%
!! AAAAAAAGTGAAAAGGTTTA+TGG - Chr5:106241836-106241855 MsG0580030073.01.T01:intron 20.0%
!! AAAAAAGTGAAAAGGTTTAT+GGG - Chr5:106241837-106241856 MsG0580030073.01.T01:intron 20.0%
!! CAAACATATCCAATAAAATT+CGG + Chr5:106240942-106240961 None:intergenic 20.0%
!! CCAAACAAAATATATTTACA+TGG - Chr5:106241103-106241122 MsG0580030073.01.T01:intron 20.0%
!! TGTTTCTTATATATGGATAT+GGG + Chr5:106241883-106241902 None:intergenic 20.0%
!! TTTGTTTGCATATATTTGAA+GGG - Chr5:106241632-106241651 MsG0580030073.01.T01:intron 20.0%
!!! AAAAAAAAACAGTTCACTTT+TGG + Chr5:106241017-106241036 None:intergenic 20.0%
!!! AATTTTCCAAATACACATTA+TGG - Chr5:106242397-106242416 MsG0580030073.01.T01:CDS 20.0%
!!! ATAGATTTTTTTTAAAGGAG+AGG - Chr5:106241288-106241307 MsG0580030073.01.T01:intron 20.0%
!!! ATTTTCCAAATACACATTAT+GGG - Chr5:106242398-106242417 MsG0580030073.01.T01:CDS 20.0%
!!! CCATGTAAATATATTTTGTT+TGG + Chr5:106241106-106241125 None:intergenic 20.0%
!!! TAGATTTTTTTTAAAGGAGA+GGG - Chr5:106241289-106241308 MsG0580030073.01.T01:intron 20.0%
!!! TTATTTTAACCCATAACAAA+CGG - Chr5:106241538-106241557 MsG0580030073.01.T01:intron 20.0%
!!! TTGTTTCTTATATATGGATA+TGG + Chr5:106241884-106241903 None:intergenic 20.0%
!!! TTTGCTTTGTTTCTTATATA+TGG + Chr5:106241890-106241909 None:intergenic 20.0%
!!! TTTTGTTTGCATATATTTGA+AGG - Chr5:106241631-106241650 MsG0580030073.01.T01:intron 20.0%
! GTGTATTTGGAAAATTACAT+GGG + Chr5:106242393-106242412 None:intergenic 25.0%
! TGTGTATTTGGAAAATTACA+TGG + Chr5:106242394-106242413 None:intergenic 25.0%
!! CGAATTTTATTGGATATGTT+TGG - Chr5:106240940-106240959 MsG0580030073.01.T01:intron 25.0%
!!! AACACATAATCTAGTTTGTT+TGG + Chr5:106241428-106241447 None:intergenic 25.0%
!!! CTTTTTGGGTTATTATACAA+TGG + Chr5:106241064-106241083 None:intergenic 25.0%
!!! TATATTTTGTTTGGTAGATC+AGG + Chr5:106241097-106241116 None:intergenic 25.0%
!!! TATTTTAACCCATAACAAAC+GGG - Chr5:106241539-106241558 MsG0580030073.01.T01:intron 25.0%
!!! TTTGCGATTTTAATTTGGAT+CGG + Chr5:106240889-106240908 None:intergenic 25.0%
!!! TTTTGTTTTTGTTTGTGAAC+AGG - Chr5:106242254-106242273 MsG0580030073.01.T01:intron 25.0%
AATGACAAAGTGAAATACCT+TGG + Chr5:106242179-106242198 None:intergenic 30.0%
ATCTAACTCAAAAAGTTTCG+AGG + Chr5:106240673-106240692 None:intergenic 30.0%
ATGACAAAGTGAAATACCTT+GGG + Chr5:106242178-106242197 None:intergenic 30.0%
TCAAATATGGGTCAGATAAT+AGG + Chr5:106241774-106241793 None:intergenic 30.0%
TCTCTGATCTATTCATTTCT+CGG - Chr5:106242031-106242050 MsG0580030073.01.T01:intron 30.0%
! TCAGGTAAATAAGTGCTTTT+TGG + Chr5:106241079-106241098 None:intergenic 30.0%
! TTTGGGTTATTATACAATGG+TGG + Chr5:106241061-106241080 None:intergenic 30.0%
!! CAGGTAAATAAGTGCTTTTT+GGG + Chr5:106241078-106241097 None:intergenic 30.0%
!! TTTGAGATTTTCAGCAACAA+TGG - Chr5:106241976-106241995 MsG0580030073.01.T01:intron 30.0%
!!! ATCGGTTTGCGATTTTAATT+TGG + Chr5:106240894-106240913 None:intergenic 30.0%
!!! GATTTTAATTTGGATCGGTT+TGG + Chr5:106240884-106240903 None:intergenic 30.0%
AAACAACCTGCACTTTCAAT+AGG + Chr5:106240717-106240736 None:intergenic 35.0%
ACAGACCCATAATGTGTATT+TGG + Chr5:106242406-106242425 None:intergenic 35.0%
AGCAACGAATGAATTTGACA+TGG + Chr5:106242135-106242154 None:intergenic 35.0%
ATACACATTATGGGTCTGTT+TGG - Chr5:106242407-106242426 MsG0580030073.01.T01:CDS 35.0%
CAATCAAGAGGTTTCTTGTT+AGG - Chr5:106242283-106242302 MsG0580030073.01.T01:intron 35.0%
CATTACAACAACAGTTGACA+TGG - Chr5:106240770-106240789 MsG0580030073.01.T01:intron 35.0%
GTCAACTGTTGTTGTAATGT+TGG + Chr5:106240769-106240788 None:intergenic 35.0%
TAGACATTTAAGCGCAGAAA+TGG - Chr5:106242085-106242104 MsG0580030073.01.T01:intron 35.0%
TGAGTTAGATGAATCATCAG+AGG - Chr5:106240683-106240702 MsG0580030073.01.T01:CDS 35.0%
! ATTCTTCCTATTGAAAGTGC+AGG - Chr5:106240708-106240727 MsG0580030073.01.T01:intron 35.0%
! ATTGAAAGTGCAGGTTGTTT+GGG - Chr5:106240717-106240736 MsG0580030073.01.T01:intron 35.0%
! CGCACAAAACCGAATTTTAT+TGG - Chr5:106240930-106240949 MsG0580030073.01.T01:intron 35.0%
! TATTGAAAGTGCAGGTTGTT+TGG - Chr5:106240716-106240735 MsG0580030073.01.T01:intron 35.0%
! TTGTGCGATTTTCGGTTTTT+TGG + Chr5:106240917-106240936 None:intergenic 35.0%
!! ATTCGGTTTTGTGCGATTTT+CGG + Chr5:106240925-106240944 None:intergenic 35.0%
!! CGATTTTCGGTTTTTTGGAT+CGG + Chr5:106240912-106240931 None:intergenic 35.0%
!! TTTTGTTTGTGAACAGGACA+AGG - Chr5:106242260-106242279 MsG0580030073.01.T01:intron 35.0%
AAAAACCGATCCAATCCAAG+CGG + Chr5:106240983-106241002 None:intergenic 40.0%
ACAACAACAGTTGACATGGT+TGG - Chr5:106240774-106240793 MsG0580030073.01.T01:intron 40.0%
ACAAGCAAAGGAGAAAGCAA+AGG - Chr5:106241952-106241971 MsG0580030073.01.T01:intron 40.0%
ACATTTAAGCGCAGAAATGG+AGG - Chr5:106242088-106242107 MsG0580030073.01.T01:intron 40.0%
AGTGAAAAGGTTTATGGGCT+TGG - Chr5:106241842-106241861 MsG0580030073.01.T01:intron 40.0%
AGTTTATCCACCTCACAACT+AGG - Chr5:106240835-106240854 MsG0580030073.01.T01:CDS 40.0%
GTGAAAAGGTTTATGGGCTT+GGG - Chr5:106241843-106241862 MsG0580030073.01.T01:intron 40.0%
GTTGTGAGGTGGATAAACTT+AGG + Chr5:106240834-106240853 None:intergenic 40.0%
TAGTTGTACTCCTAGTTGTG+AGG + Chr5:106240848-106240867 None:intergenic 40.0%
! TCACAACTAGGAGTACAACT+AGG - Chr5:106240847-106240866 MsG0580030073.01.T01:CDS 40.0%
!! CTTGGATTGGATCGGTTTTT+GGG - Chr5:106240983-106241002 MsG0580030073.01.T01:intron 40.0%
!! TGACATGGTTGGTCTATATG+CGG - Chr5:106240785-106240804 MsG0580030073.01.T01:intron 40.0%
!! TTTGTTGGCCCGTTTGTTAT+GGG + Chr5:106241550-106241569 None:intergenic 40.0%
!! TTTTGTTGGCCCGTTTGTTA+TGG + Chr5:106241551-106241570 None:intergenic 40.0%
!!! AGGTTGTTTGGGTTTTGGAA+GGG - Chr5:106240728-106240747 MsG0580030073.01.T01:intron 40.0%
AACAGGACAAGGCAATCAAG+AGG - Chr5:106242271-106242290 MsG0580030073.01.T01:intron 45.0%
AATGGAGGCCGTAACAAACA+TGG - Chr5:106242103-106242122 MsG0580030073.01.T01:intron 45.0%
AGCTGCAGTTAGAGATGTTC+AGG - Chr5:106242320-106242339 MsG0580030073.01.T01:intron 45.0%
CTGGTTAAGGAAGCCAAAAG+AGG - Chr5:106241681-106241700 MsG0580030073.01.T01:intron 45.0%
CTTTCTCCTTTGCTTGTTCG+CGG + Chr5:106241949-106241968 None:intergenic 45.0%
GACATTTCGTGAAAACCGCT+TGG - Chr5:106240965-106240984 MsG0580030073.01.T01:intron 45.0%
TTCATTCGTTGCTCCAACTG+TGG - Chr5:106242142-106242161 MsG0580030073.01.T01:intron 45.0%
TTCGTGAAAACCGCTTGGAT+TGG - Chr5:106240970-106240989 MsG0580030073.01.T01:intron 45.0%
TTGTACTCCTAGTTGTGAGG+TGG + Chr5:106240845-106240864 None:intergenic 45.0%
! GAAAACCGCTTGGATTGGAT+CGG - Chr5:106240975-106240994 MsG0580030073.01.T01:intron 45.0%
!! AGTGCAGGTTGTTTGGGTTT+TGG - Chr5:106240723-106240742 MsG0580030073.01.T01:intron 45.0%
!! GCTTGGATTGGATCGGTTTT+TGG - Chr5:106240982-106241001 MsG0580030073.01.T01:intron 45.0%
!!! CAGGTTGTTTGGGTTTTGGA+AGG - Chr5:106240727-106240746 MsG0580030073.01.T01:intron 45.0%
!!! AAAAAATAGATTTTTTTTAA+AGG - Chr5:106241283-106241302 MsG0580030073.01.T01:intron 5.0%
CTGTGGTAAATGCTGTCCCA+AGG - Chr5:106242159-106242178 MsG0580030073.01.T01:intron 50.0%
GGGACAGCATTTACCACAGT+TGG + Chr5:106242158-106242177 None:intergenic 50.0%
! GGCCACGACCATGTTTGTTA+CGG + Chr5:106242114-106242133 None:intergenic 50.0%
!!! GGGTTTTGGAAGGGTCATTG+TGG - Chr5:106240737-106240756 MsG0580030073.01.T01:intron 50.0%
CTTCCTTAACCAGTGTCCCC+GGG + Chr5:106241674-106241693 None:intergenic 55.0%
GCTTCCTTAACCAGTGTCCC+CGG + Chr5:106241675-106241694 None:intergenic 55.0%
GGCCGTAACAAACATGGTCG+TGG - Chr5:106242109-106242128 MsG0580030073.01.T01:intron 55.0%
GGTCATGTTAACCAGTGCCC+CGG - Chr5:106241653-106241672 MsG0580030073.01.T01:intron 55.0%
GTCATGTTAACCAGTGCCCC+GGG - Chr5:106241654-106241673 MsG0580030073.01.T01:intron 55.0%
TAGCTGCCGCGAACAAGCAA+AGG - Chr5:106241940-106241959 MsG0580030073.01.T01:intron 55.0%
TCATGTTAACCAGTGCCCCG+GGG - Chr5:106241655-106241674 MsG0580030073.01.T01:intron 55.0%
TTCCTTAACCAGTGTCCCCG+GGG + Chr5:106241673-106241692 None:intergenic 55.0%
TTGCTTGTTCGCGGCAGCTA+GGG + Chr5:106241940-106241959 None:intergenic 55.0%
TTTGCTTGTTCGCGGCAGCT+AGG + Chr5:106241941-106241960 None:intergenic 55.0%
! TGCCCCGGGGACACTGGTTA+AGG - Chr5:106241668-106241687 MsG0580030073.01.T01:intron 65.0%
AACCAGTGCCCCGGGGACAC+TGG - Chr5:106241662-106241681 MsG0580030073.01.T01:intron 70.0%
AACCAGTGTCCCCGGGGCAC+TGG + Chr5:106241667-106241686 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 106240660 106242504 106240660 ID=MsG0580030073.01;Name=MsG0580030073.01
Chr5 mRNA 106240660 106242504 106240660 ID=MsG0580030073.01.T01;Parent=MsG0580030073.01;Name=MsG0580030073.01.T01;_AED=0.67;_eAED=0.74;_QI=0|0.33|0.5|0.75|0.66|0.75|4|0|119
Chr5 exon 106242373 106242504 106242373 ID=MsG0580030073.01.T01:exon:17181;Parent=MsG0580030073.01.T01
Chr5 exon 106240984 106241076 106240984 ID=MsG0580030073.01.T01:exon:17180;Parent=MsG0580030073.01.T01
Chr5 exon 106240804 106240888 106240804 ID=MsG0580030073.01.T01:exon:17179;Parent=MsG0580030073.01.T01
Chr5 exon 106240660 106240709 106240660 ID=MsG0580030073.01.T01:exon:17178;Parent=MsG0580030073.01.T01
Chr5 CDS 106242373 106242504 106242373 ID=MsG0580030073.01.T01:cds;Parent=MsG0580030073.01.T01
Chr5 CDS 106240984 106241076 106240984 ID=MsG0580030073.01.T01:cds;Parent=MsG0580030073.01.T01
Chr5 CDS 106240804 106240888 106240804 ID=MsG0580030073.01.T01:cds;Parent=MsG0580030073.01.T01
Chr5 CDS 106240660 106240709 106240660 ID=MsG0580030073.01.T01:cds;Parent=MsG0580030073.01.T01
Gene Sequence

>MsG0580030073.01.T01

ATGGAAGGATCCTCGAAACTTTTTGAGTTAGATGAATCATCAGAGGTCATTCTTCCTATTGAAAGTGCAGGTTGTTTGGGTTTTGGAAGGGTCATTGTGGCACTAGCCAACATTACAACAACAGTTGACATGACATTTAAGCGCAGAAATGGAGGCCGTAACAAACATGGTCGTGGCCATGTCAAATTCATTCGTTGCTCCAACTGTGGTAAATGCTGTCCCAAGGACAAGGCAATCAAGAGGTTTCTTGTTAGGAACATTGTTGAACAAGCTGCAGTTAGAGATGTTCAGGAAGCATGTGTTTATGACCTTTATGCAAAACAGATTATGCAAATTAATAAGCTTTTATTCATTTCATAA

Protein sequence

>MsG0580030073.01.T01

MEGSSKLFELDESSEVILPIESAGCLGFGRVIVALANITTTVDMTFKRRNGGRNKHGRGHVKFIRCSNCGKCCPKDKAIKRFLVRNIVEQAAVRDVQEACVYDLYAKQIMQINKLLFIS*