AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048922.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048922.01.T01 MTR_1g058250 96.575 146 3 1 1 146 1 144 1.15e-99 282
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048922.01.T01 AT5G04800 76.667 150 22 2 1 146 1 141 3.71e-77 225
MsG0080048922.01.T01 AT5G04800 76.667 150 22 2 1 146 1 141 3.71e-77 225
MsG0080048922.01.T01 AT5G04800 76.667 150 22 2 1 146 1 141 3.71e-77 225
MsG0080048922.01.T01 AT5G04800 76.667 150 22 2 1 146 1 141 3.71e-77 225
MsG0080048922.01.T01 AT2G04390 78.912 147 24 2 1 146 1 141 3.11e-76 223
MsG0080048922.01.T01 AT2G05220 78.767 146 25 2 1 146 1 140 4.82e-76 223
MsG0080048922.01.T01 AT2G05220 78.767 146 25 2 1 146 1 140 4.82e-76 223
MsG0080048922.01.T01 AT3G10610 80.576 139 20 2 1 135 1 136 1.68e-74 219

Find 45 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATGATTTCTTCACGGTTT+TGG 0.256241 contig540end:-25895 MsG0080048922.01.T01:intergenic
CGAGATTCCCGGTGTTGTTC+AGG 0.275016 contig540end:+26214 MsG0080048922.01.T01:CDS
GCTGCTGGTGGTGGTGGTGC+TGG 0.283520 contig540end:+26284 MsG0080048922.01.T01:CDS
CGTCTTCGCAACAAGATTGC+TGG 0.339095 contig540end:+26011 MsG0080048922.01.T01:CDS
TTCCTGATGTTTCTGCTATT+AGG 0.341443 contig540end:+26132 MsG0080048922.01.T01:CDS
TTCTTCAAGGATCTTCTTGT+TGG 0.343538 contig540end:-25967 MsG0080048922.01.T01:intergenic
TTGTTGCGAAGACGCTTTGA+TGG 0.348895 contig540end:-26002 MsG0080048922.01.T01:intergenic
ATCCCATTCGGTCGTGGTGC+TGG 0.359143 contig540end:+26260 MsG0080048922.01.T01:CDS
CCGAATGGGATCTGCTGAAC+AGG 0.361093 contig540end:-26248 MsG0080048922.01.T01:intergenic
GCTGGTGCTGCTGGTGGTGG+TGG 0.367461 contig540end:+26278 MsG0080048922.01.T01:CDS
GGGATCTGCTGAACAGGAAC+AGG 0.372363 contig540end:-26242 MsG0080048922.01.T01:intergenic
GGTCGTGGTGCTGGTGCTGC+TGG 0.373909 contig540end:+26269 MsG0080048922.01.T01:CDS
CTTGGTATGTCCGAGATTCC+CGG 0.385581 contig540end:+26203 MsG0080048922.01.T01:CDS
CTGGTGGTGGTGGTGCTGGA+AGG 0.390747 contig540end:+26288 MsG0080048922.01.T01:CDS
CCTTCTGAATACGTTTCATA+AGG 0.423532 contig540end:-26046 MsG0080048922.01.T01:intergenic
CTAGACATGCTTGCTGCTCT+TGG 0.432500 contig540end:+26185 MsG0080048922.01.T01:CDS
GGTGCTGGTGCTGCTGGTGG+TGG 0.435254 contig540end:+26275 MsG0080048922.01.T01:CDS
TTCAATGAAATTCCACGAAC+AGG 0.443778 contig540end:-26071 MsG0080048922.01.T01:intergenic
CTGAACAACACCGGGAATCT+CGG 0.461504 contig540end:-26213 MsG0080048922.01.T01:intergenic
GAATCAAAGCAACTTCTTCA+AGG 0.471503 contig540end:-25980 MsG0080048922.01.T01:intergenic
CCTGTTCAGCAGATCCCATT+CGG 0.473555 contig540end:+26248 MsG0080048922.01.T01:CDS
CAAGAGCAGCAAGCATGTCT+AGG 0.477650 contig540end:-26184 MsG0080048922.01.T01:intergenic
GTGGTGGTGGTGCTGGAAGG+AGG 0.481041 contig540end:+26291 MsG0080048922.01.T01:CDS
TGATCATATTGAGGTTGATA+AGG 0.481558 contig540end:+26157 MsG0080048922.01.T01:CDS
CAAGGATCTTCTTGTTGGTG+TGG 0.483634 contig540end:-25962 MsG0080048922.01.T01:intergenic
TTGACGAGATGATTTCTTCA+CGG 0.484691 contig540end:-25901 MsG0080048922.01.T01:intergenic
CACCAGCACCACGACCGAAT+GGG 0.505188 contig540end:-26262 MsG0080048922.01.T01:intergenic
GTCCTAATAGCAGAAACATC+AGG 0.505221 contig540end:-26134 MsG0080048922.01.T01:intergenic
GGAGGAGCGTGAGCGTCGTA+TGG 0.521163 contig540end:+26103 MsG0080048922.01.T01:CDS
TTTGGTTCGGACTCGACCCA+TGG 0.522820 contig540end:-25877 MsG0080048922.01.T01:intergenic
ATTCAGAAGGGACCTGTTCG+TGG 0.526647 contig540end:+26059 MsG0080048922.01.T01:CDS
CGTGGTGCTGGTGCTGCTGG+TGG 0.530900 contig540end:+26272 MsG0080048922.01.T01:CDS
CAGCAGATCCCATTCGGTCG+TGG 0.540344 contig540end:+26254 MsG0080048922.01.T01:CDS
GTGGAAATCGAGTGTCATCT+TGG 0.542107 contig540end:-25943 MsG0080048922.01.T01:intergenic
TATTAGGACTGATCATATTG+AGG 0.542204 contig540end:+26148 MsG0080048922.01.T01:CDS
GCACCAGCACCACGACCGAA+TGG 0.558262 contig540end:-26263 MsG0080048922.01.T01:intergenic
AAGAGCAGCAAGCATGTCTA+GGG 0.560169 contig540end:-26183 MsG0080048922.01.T01:intergenic
TTCATTGAAGCTGCAAGAGG+AGG 0.567846 contig540end:+26085 MsG0080048922.01.T01:CDS
CCTTATGAAACGTATTCAGA+AGG 0.588661 contig540end:+26046 MsG0080048922.01.T01:CDS
AGGGTCAACCTGAACAACAC+CGG 0.593915 contig540end:-26222 MsG0080048922.01.T01:intergenic
GGGTCAACCTGAACAACACC+GGG 0.608103 contig540end:-26221 MsG0080048922.01.T01:intergenic
AATACGTTTCATAAGGTGAG+TGG 0.609703 contig540end:-26039 MsG0080048922.01.T01:intergenic
AATTTCATTGAAGCTGCAAG+AGG 0.634258 contig540end:+26082 MsG0080048922.01.T01:CDS
CTTATGAAACGTATTCAGAA+GGG 0.635445 contig540end:+26047 MsG0080048922.01.T01:CDS
GGATCTGCTGAACAGGAACA+GGG 0.673645 contig540end:-26241 MsG0080048921.01.T01:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTTATGAAACGTATTCAGAA+GGG + contig540end:26047-26066 MsG0080048922.01.T01:CDS 30.0%
TATTAGGACTGATCATATTG+AGG + contig540end:26148-26167 MsG0080048922.01.T01:CDS 30.0%
!! TGATCATATTGAGGTTGATA+AGG + contig540end:26157-26176 MsG0080048922.01.T01:CDS 30.0%
AATACGTTTCATAAGGTGAG+TGG - contig540end:26042-26061 MsG0080048921.01.T01:intergenic 35.0%
AATTTCATTGAAGCTGCAAG+AGG + contig540end:26082-26101 MsG0080048922.01.T01:CDS 35.0%
CCTTATGAAACGTATTCAGA+AGG + contig540end:26046-26065 MsG0080048922.01.T01:CDS 35.0%
CCTTCTGAATACGTTTCATA+AGG - contig540end:26049-26068 MsG0080048922.01.T01:intergenic 35.0%
GAATCAAAGCAACTTCTTCA+AGG - contig540end:25983-26002 MsG0080048922.01.T01:intergenic 35.0%
TTCAATGAAATTCCACGAAC+AGG - contig540end:26074-26093 MsG0080048922.01.T01:intergenic 35.0%
TTCTTCAAGGATCTTCTTGT+TGG - contig540end:25970-25989 MsG0080048922.01.T01:intergenic 35.0%
TTGACGAGATGATTTCTTCA+CGG - contig540end:25904-25923 MsG0080048922.01.T01:intergenic 35.0%
! AGATGATTTCTTCACGGTTT+TGG - contig540end:25898-25917 MsG0080048922.01.T01:intergenic 35.0%
! TTCCTGATGTTTCTGCTATT+AGG + contig540end:26132-26151 MsG0080048922.01.T01:CDS 35.0%
!!! ATTTCTTCACGGTTTTGGTT+CGG - contig540end:25893-25912 MsG0080048922.01.T01:intergenic 35.0%
GTCCTAATAGCAGAAACATC+AGG - contig540end:26137-26156 MsG0080048922.01.T01:intergenic 40.0%
AAGAGCAGCAAGCATGTCTA+GGG - contig540end:26186-26205 MsG0080048921.01.T01:intergenic 45.0%
CAAGGATCTTCTTGTTGGTG+TGG - contig540end:25965-25984 MsG0080048922.01.T01:intergenic 45.0%
GTGGAAATCGAGTGTCATCT+TGG - contig540end:25946-25965 MsG0080048922.01.T01:intergenic 45.0%
TTCATTGAAGCTGCAAGAGG+AGG + contig540end:26085-26104 MsG0080048922.01.T01:CDS 45.0%
TTGTTGCGAAGACGCTTTGA+TGG - contig540end:26005-26024 MsG0080048922.01.T01:intergenic 45.0%
AGGGTCAACCTGAACAACAC+CGG - contig540end:26225-26244 MsG0080048922.01.T01:intergenic 50.0%
ATTCAGAAGGGACCTGTTCG+TGG + contig540end:26059-26078 MsG0080048922.01.T01:CDS 50.0%
CAAGAGCAGCAAGCATGTCT+AGG - contig540end:26187-26206 MsG0080048922.01.T01:intergenic 50.0%
CCTGTTCAGCAGATCCCATT+CGG + contig540end:26248-26267 MsG0080048922.01.T01:CDS 50.0%
CGTCTTCGCAACAAGATTGC+TGG + contig540end:26011-26030 MsG0080048922.01.T01:CDS 50.0%
CTGAACAACACCGGGAATCT+CGG - contig540end:26216-26235 MsG0080048922.01.T01:intergenic 50.0%
CTTGGTATGTCCGAGATTCC+CGG + contig540end:26203-26222 MsG0080048922.01.T01:CDS 50.0%
GGATCTGCTGAACAGGAACA+GGG - contig540end:26244-26263 MsG0080048922.01.T01:intergenic 50.0%
! CTAGACATGCTTGCTGCTCT+TGG + contig540end:26185-26204 MsG0080048922.01.T01:CDS 50.0%
CCGAATGGGATCTGCTGAAC+AGG - contig540end:26251-26270 MsG0080048922.01.T01:intergenic 55.0%
GGGATCTGCTGAACAGGAAC+AGG - contig540end:26245-26264 MsG0080048922.01.T01:intergenic 55.0%
GGGTCAACCTGAACAACACC+GGG - contig540end:26224-26243 MsG0080048922.01.T01:intergenic 55.0%
!! CGAGATTCCCGGTGTTGTTC+AGG + contig540end:26214-26233 MsG0080048922.01.T01:CDS 55.0%
ATCCCATTCGGTCGTGGTGC+TGG + contig540end:26260-26279 MsG0080048922.01.T01:CDS 60.0%
CACCAGCACCACGACCGAAT+GGG - contig540end:26265-26284 MsG0080048922.01.T01:intergenic 60.0%
CAGCAGATCCCATTCGGTCG+TGG + contig540end:26254-26273 MsG0080048922.01.T01:CDS 60.0%
GCACCAGCACCACGACCGAA+TGG - contig540end:26266-26285 MsG0080048922.01.T01:intergenic 65.0%
! GGAGGAGCGTGAGCGTCGTA+TGG + contig540end:26103-26122 MsG0080048922.01.T01:CDS 65.0%
!! CTGGTGGTGGTGGTGCTGGA+AGG + contig540end:26288-26307 MsG0080048922.01.T01:CDS 65.0%
!! GTGGTGGTGGTGCTGGAAGG+AGG + contig540end:26291-26310 MsG0080048922.01.T01:CDS 65.0%
!! CGTGGTGCTGGTGCTGCTGG+TGG + contig540end:26272-26291 MsG0080048922.01.T01:CDS 70.0%
!! GCTGCTGGTGGTGGTGGTGC+TGG + contig540end:26284-26303 MsG0080048922.01.T01:CDS 70.0%
!! GCTGGTGCTGCTGGTGGTGG+TGG + contig540end:26278-26297 MsG0080048922.01.T01:CDS 70.0%
!! GGTCGTGGTGCTGGTGCTGC+TGG + contig540end:26269-26288 MsG0080048922.01.T01:CDS 70.0%
!! GGTGCTGGTGCTGCTGGTGG+TGG + contig540end:26275-26294 MsG0080048922.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
contig540end gene 25879 26319 25879 ID=MsG0080048922.01;Name=MsG0080048922.01
contig540end mRNA 25879 26319 25879 ID=MsG0080048922.01.T01;Parent=MsG0080048922.01;Name=MsG0080048922.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|146
contig540end exon 25879 26319 25879 ID=MsG0080048922.01.T01:exon:22141;Parent=MsG0080048922.01.T01
contig540end CDS 25879 26319 25879 ID=MsG0080048922.01.T01:cds;Parent=MsG0080048922.01.T01
Gene Sequence

>MsG0080048922.01.T01

ATGGGTCGAGTCCGAACCAAAACCGTGAAGAAATCATCTCGTCAAGTCATTGAGCGTTATTACTCCAAGATGACACTCGATTTCCACACCAACAAGAAGATCCTTGAAGAAGTTGCTTTGATTCCATCAAAGCGTCTTCGCAACAAGATTGCTGGTTTTTCCACTCACCTTATGAAACGTATTCAGAAGGGACCTGTTCGTGGAATTTCATTGAAGCTGCAAGAGGAGGAGCGTGAGCGTCGTATGGACTTCGTTCCTGATGTTTCTGCTATTAGGACTGATCATATTGAGGTTGATAAGGAAACCCTAGACATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGTGTTGTTCAGGTTGACCCTGTTCCTGTTCAGCAGATCCCATTCGGTCGTGGTGCTGGTGCTGCTGGTGGTGGTGGTGCTGGAAGGAGGTTTTAA

Protein sequence

>MsG0080048922.01.T01

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQVDPVPVQQIPFGRGAGAAGGGGAGRRF*