AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046269.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046269.01.T01 MTR_4g104570 98.148 54 1 0 64 117 147 200 7.91e-33 114
MsG0880046269.01.T01 MTR_4g104570 89.744 39 4 0 25 63 1 39 1.17e-17 75.1
MsG0880046269.01.T01 MTR_4g104540 96.296 54 2 0 64 117 378 431 4.76e-30 111
MsG0880046269.01.T01 MTR_4g104540 90.000 40 4 0 24 63 231 270 1.03e-17 77.4
MsG0880046269.01.T01 MTR_4g104550 67.857 56 15 1 64 116 439 494 2.74e-20 85.1
MsG0880046269.01.T01 MTR_4g104550 77.500 40 9 0 24 63 292 331 1.34e-14 68.9
MsG0880046269.01.T01 MTR_4g104610 74.000 50 12 1 64 113 448 496 3.34e-18 79.0
MsG0880046269.01.T01 MTR_4g104610 87.179 39 5 0 24 62 301 339 1.61e-15 71.6
MsG0880046269.01.T01 MTR_4g089030 63.462 52 17 1 64 113 464 515 1.24e-15 71.6
MsG0880046269.01.T01 MTR_4g089055 61.538 52 18 1 64 113 462 513 3.60e-15 70.5
MsG0880046269.01.T01 MTR_4g089055 49.315 73 29 2 12 76 296 368 2.73e-12 62.4
MsG0880046269.01.T01 MTR_4g089025 61.538 52 18 1 64 113 465 516 6.20e-15 69.7
MsG0880046269.01.T01 MTR_0022s0290 57.407 54 20 1 65 115 464 517 1.27e-14 68.9
MsG0880046269.01.T01 MTR_4g089020 63.462 52 17 1 64 113 440 491 4.64e-14 67.4
MsG0880046269.01.T01 MTR_4g104640 62.500 40 15 0 23 62 316 355 3.00e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046269.01.T01 AT3G48280 50.000 52 24 1 65 114 436 487 2.15e-11 59.7
MsG0880046269.01.T01 AT3G48300 38.372 86 40 1 24 109 285 357 2.37e-11 59.7
MsG0880046269.01.T01 AT3G48300 38.372 86 40 1 24 109 287 359 2.37e-11 59.7
MsG0880046269.01.T01 AT2G30750 48.148 54 28 0 24 77 294 347 5.53e-11 58.5
MsG0880046269.01.T01 AT2G30770 46.296 54 29 0 24 77 294 347 7.48e-11 58.2

Find 28 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATTTCTTCATGGTATT+TGG 0.107830 8:+71343523 None:intergenic
ACCTAGTACATCAATTTAAC+TGG 0.251658 8:-71343123 MsG0880046269.01.T01:CDS
TTGGACATGTCTGAATCTTT+TGG 0.261216 8:-71343076 MsG0880046269.01.T01:CDS
TTCTTCTTTCAATTTCTTCA+TGG 0.271619 8:+71343516 None:intergenic
TTCTTGGTTTAGTTCTTGTT+AGG 0.284060 8:+71343010 None:intergenic
TCATAGAAAGATTCCTCTTA+TGG 0.303196 8:-71343044 MsG0880046269.01.T01:CDS
AAGCCAAGTGATCTGTTCTT+TGG 0.306176 8:+71344483 None:intergenic
CCACTCTAGCAAAGTGGATA+TGG 0.323275 8:+71343570 None:intergenic
TCTTGGTTTAGTTCTTGTTA+GGG 0.381697 8:+71343011 None:intergenic
ATTCAGGACATGTTTATTGC+AGG 0.383065 8:-71343601 MsG0880046269.01.T01:intron
CCCAGTTAAATTGATGTACT+AGG 0.400246 8:+71343122 None:intergenic
GAAGTAAGTAAAGTGGCTAA+TGG 0.413940 8:-71343496 MsG0880046269.01.T01:intron
GAAAGAAGAAGTAAGTAAAG+TGG 0.480184 8:-71343503 MsG0880046269.01.T01:CDS
GGTCAATGACAGAACTCTTA+AGG 0.482595 8:-71343549 MsG0880046269.01.T01:CDS
ACCTGGTGGTGCTGAAGGAT+TGG 0.501420 8:-71343095 MsG0880046269.01.T01:CDS
CAATTTAACTGGGAATTACC+TGG 0.504499 8:-71343112 MsG0880046269.01.T01:CDS
TTGGCTTTCCTATGACAAAA+CGG 0.528506 8:-71344467 MsG0880046269.01.T01:intron
GTTAAATTGATGTACTAGGT+TGG 0.534321 8:+71343126 None:intergenic
CCATATCCACTTTGCTAGAG+TGG 0.537370 8:-71343570 MsG0880046269.01.T01:CDS
GGGGTTGCAATTGCCATAAG+AGG 0.549821 8:+71343031 None:intergenic
CTTGGTTTAGTTCTTGTTAG+GGG 0.554960 8:+71343012 None:intergenic
GATCCAAAGAACAGATCACT+TGG 0.561454 8:-71344486 MsG0880046269.01.T01:CDS
TCCAATCCTTCAGCACCACC+AGG 0.562498 8:+71343094 None:intergenic
GAATTACCTGGTGGTGCTGA+AGG 0.569484 8:-71343100 MsG0880046269.01.T01:CDS
CATTGACCACTCTAGCAAAG+TGG 0.569641 8:+71343564 None:intergenic
CCTAGTACATCAATTTAACT+GGG 0.605070 8:-71343122 MsG0880046269.01.T01:CDS
GTCCTGGGCAGTGTATGCTA+CGG 0.660471 8:-71343170 MsG0880046269.01.T01:intron
TTTAACTGGGAATTACCTGG+TGG 0.730778 8:-71343109 MsG0880046269.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGTAAAAATTTATTAAA+GGG - Chr8:71343164-71343183 MsG0880046269.01.T01:intron 10.0%
!! GAGAAGTAAAAATTTATTAA+AGG - Chr8:71343163-71343182 MsG0880046269.01.T01:intron 15.0%
!! GATTAAATAAGATTATAAGA+AGG + Chr8:71343361-71343380 None:intergenic 15.0%
!! TATGTATAAGTGTATTAATT+TGG + Chr8:71343268-71343287 None:intergenic 15.0%
!!! ATTAAATCTTTATTTGATCA+TGG - Chr8:71343449-71343468 MsG0880046269.01.T01:intron 15.0%
!!! GAATATTTATTTTATGTACT+TGG + Chr8:71343496-71343515 None:intergenic 15.0%
!! TTATATTATCCTATTGTGAT+GGG + Chr8:71343429-71343448 None:intergenic 20.0%
!! TTTATATTATCCTATTGTGA+TGG + Chr8:71343430-71343449 None:intergenic 20.0%
!!! ATTTTAACGAATGTTATAGT+TGG + Chr8:71343314-71343333 None:intergenic 20.0%
!!! GTACTTGGTTAAATTTTTAT+TGG + Chr8:71343481-71343500 None:intergenic 20.0%
! AGACAAGATATCAAACTAAA+TGG - Chr8:71344171-71344190 MsG0880046269.01.T01:intron 25.0%
! ATTATATTAATATGATGCGC+AGG + Chr8:71343771-71343790 None:intergenic 25.0%
! TCACTCACAATTAAAATCAT+TGG - Chr8:71343739-71343758 MsG0880046269.01.T01:intron 25.0%
! TGAGTAGTTCAATATGTTAA+AGG - Chr8:71344295-71344314 MsG0880046269.01.T01:intron 25.0%
! TTATATTAATATGATGCGCA+GGG + Chr8:71343770-71343789 None:intergenic 25.0%
! TTCAATTTCTTCATGGTATT+TGG + Chr8:71344012-71344031 None:intergenic 25.0%
! TTCTTCTTTCAATTTCTTCA+TGG + Chr8:71344019-71344038 None:intergenic 25.0%
!! ACACTTATACATAAGTGTTT+TGG - Chr8:71343275-71343294 MsG0880046269.01.T01:intron 25.0%
!! ATTTTGTTAGTAGACCTAAA+AGG + Chr8:71343640-71343659 None:intergenic 25.0%
!! CACTTATACATAAGTGTTTT+GGG - Chr8:71343276-71343295 MsG0880046269.01.T01:intron 25.0%
!!! AATGATTTTAATTGTGAGTG+AGG + Chr8:71343740-71343759 None:intergenic 25.0%
!!! TCTTGTATTTGTTTCTATTC+AGG - Chr8:71343915-71343934 MsG0880046269.01.T01:intron 25.0%
AAAAGTCCATTAAGCTTCAA+AGG + Chr8:71343138-71343157 None:intergenic 30.0%
AAAATCATACAAGTTCCTAG+AGG + Chr8:71343804-71343823 None:intergenic 30.0%
AAATGTTGTATGTCATTAGG+CGG - Chr8:71343834-71343853 MsG0880046269.01.T01:intron 30.0%
ACCTAGTACATCAATTTAAC+TGG - Chr8:71344409-71344428 MsG0880046269.01.T01:intron 30.0%
CACAATTAAAATCATTGGAC+TGG - Chr8:71343744-71343763 MsG0880046269.01.T01:intron 30.0%
CATGAAATACTTAAATGCAG+TGG - Chr8:71344093-71344112 MsG0880046269.01.T01:intron 30.0%
CCTAGTACATCAATTTAACT+GGG - Chr8:71344410-71344429 MsG0880046269.01.T01:intron 30.0%
GAAAGAAGAAGTAAGTAAAG+TGG - Chr8:71344029-71344048 MsG0880046269.01.T01:intron 30.0%
GTTAAATTGATGTACTAGGT+TGG + Chr8:71344409-71344428 None:intergenic 30.0%
TATCTATCTTTGACGAAAGA+AGG - Chr8:71343581-71343600 MsG0880046269.01.T01:CDS 30.0%
TCAAATCTTCTTCAGTTATG+TGG + Chr8:71344069-71344088 None:intergenic 30.0%
TCATAGAAAGATTCCTCTTA+TGG - Chr8:71344488-71344507 MsG0880046269.01.T01:CDS 30.0%
TCGTCAAAGATAGATATAAC+CGG + Chr8:71343577-71343596 None:intergenic 30.0%
TGCAAATGTTGTATGTCATT+AGG - Chr8:71343831-71343850 MsG0880046269.01.T01:intron 30.0%
TTCAAGTAGATAGAAGAAGA+TGG - Chr8:71343517-71343536 MsG0880046269.01.T01:CDS 30.0%
! ACTTTGTTGAAGTTTTGCTT+TGG - Chr8:71343024-71343043 MsG0880046269.01.T01:CDS 30.0%
!! TATCATCCTTTGAAGCTTAA+TGG - Chr8:71343129-71343148 MsG0880046269.01.T01:CDS 30.0%
!!! CTTTTTTCGTTTTGCATAAG+CGG + Chr8:71343693-71343712 None:intergenic 30.0%
AAGAAGGAATCTTTGACCTA+AGG - Chr8:71343597-71343616 MsG0880046269.01.T01:CDS 35.0%
AATTGAGGGATGTAAACGTA+GGG + Chr8:71344127-71344146 None:intergenic 35.0%
ACATACAACATTTGCAAGCA+TGG + Chr8:71343827-71343846 None:intergenic 35.0%
AGAAGGAATCTTTGACCTAA+GGG - Chr8:71343598-71343617 MsG0880046269.01.T01:CDS 35.0%
ATTCAGGACATGTTTATTGC+AGG - Chr8:71343931-71343950 MsG0880046269.01.T01:intron 35.0%
CAATTTAACTGGGAATTACC+TGG - Chr8:71344420-71344439 MsG0880046269.01.T01:intron 35.0%
CCCAGTTAAATTGATGTACT+AGG + Chr8:71344413-71344432 None:intergenic 35.0%
CTATGACAAAACGGTCATAA+AGG - Chr8:71343074-71343093 MsG0880046269.01.T01:CDS 35.0%
GAAGTAAGTAAAGTGGCTAA+TGG - Chr8:71344036-71344055 MsG0880046269.01.T01:intron 35.0%
GATTTCCAAGTGATTCCATT+TGG - Chr8:71344322-71344341 MsG0880046269.01.T01:intron 35.0%
! ACGAGTTTTCATCAATGCTT+GGG - Chr8:71344214-71344233 MsG0880046269.01.T01:intron 35.0%
! AGGTACTAGTAATGGAATTG+AGG + Chr8:71344142-71344161 None:intergenic 35.0%
! ATAATGTTTGAGACACCTCT+AGG - Chr8:71343786-71343805 MsG0880046269.01.T01:intron 35.0%
! CTTTATGACCGTTTTGTCAT+AGG + Chr8:71343076-71343095 None:intergenic 35.0%
! GGTACTAGTAATGGAATTGA+GGG + Chr8:71344141-71344160 None:intergenic 35.0%
! TTGGACATGTCTGAATCTTT+TGG - Chr8:71344456-71344475 MsG0880046269.01.T01:intron 35.0%
! TTGGCTTTCCTATGACAAAA+CGG - Chr8:71343065-71343084 MsG0880046269.01.T01:CDS 35.0%
AAGCCAAGTGATCTGTTCTT+TGG + Chr8:71343052-71343071 None:intergenic 40.0%
ATATCTTGTCTGCTTTCTCG+AGG + Chr8:71344162-71344181 None:intergenic 40.0%
ATGAGCAATCCCATCACAAT+AGG - Chr8:71343417-71343436 MsG0880046269.01.T01:intron 40.0%
ATGTGTAGGATCTCTTGCAA+TGG + Chr8:71344240-71344259 None:intergenic 40.0%
CTTGACGTACACTAATTAGC+CGG - Chr8:71343555-71343574 MsG0880046269.01.T01:CDS 40.0%
GAACTACTCAAGAATCTCTC+AGG + Chr8:71344285-71344304 None:intergenic 40.0%
GAATTGAGGGATGTAAACGT+AGG + Chr8:71344128-71344147 None:intergenic 40.0%
GATCCAAAGAACAGATCACT+TGG - Chr8:71343046-71343065 MsG0880046269.01.T01:CDS 40.0%
GGTCAATGACAGAACTCTTA+AGG - Chr8:71343983-71344002 MsG0880046269.01.T01:intron 40.0%
TGCAAGAGATCCTACACATT+GGG - Chr8:71344241-71344260 MsG0880046269.01.T01:intron 40.0%
TTGCAAGAGATCCTACACAT+TGG - Chr8:71344240-71344259 MsG0880046269.01.T01:intron 40.0%
TTTAACTGGGAATTACCTGG+TGG - Chr8:71344423-71344442 MsG0880046269.01.T01:intron 40.0%
! CACGAGTTTTCATCAATGCT+TGG - Chr8:71344213-71344232 MsG0880046269.01.T01:intron 40.0%
! CTTTCTCGAGGTACTAGTAA+TGG + Chr8:71344150-71344169 None:intergenic 40.0%
! GAGCTATATGTCGTCCTTTT+AGG - Chr8:71343623-71343642 MsG0880046269.01.T01:intron 40.0%
CAGCTCCAAATGGAATCACT+TGG + Chr8:71344330-71344349 None:intergenic 45.0%
CATTGACCACTCTAGCAAAG+TGG + Chr8:71343971-71343990 None:intergenic 45.0%
CCACTCTAGCAAAGTGGATA+TGG + Chr8:71343965-71343984 None:intergenic 45.0%
CCATATCCACTTTGCTAGAG+TGG - Chr8:71343962-71343981 MsG0880046269.01.T01:intron 45.0%
CGACATATAGCTCAACCCTT+AGG + Chr8:71343616-71343635 None:intergenic 45.0%
! CAAGTGATTCCATTTGGAGC+TGG - Chr8:71344328-71344347 MsG0880046269.01.T01:intron 45.0%
! TCCATTTGGAGCTGGTAGAA+GGG - Chr8:71344336-71344355 MsG0880046269.01.T01:intron 45.0%
! TTCCATTTGGAGCTGGTAGA+AGG - Chr8:71344335-71344354 MsG0880046269.01.T01:intron 45.0%
!! TCAGGTTGATCCCAATGTGT+AGG + Chr8:71344254-71344273 None:intergenic 45.0%
ATCTCTCAGGCTGAACTCTC+AGG + Chr8:71344272-71344291 None:intergenic 50.0%
GGGGTTGCAATTGCCATAAG+AGG + Chr8:71344504-71344523 None:intergenic 50.0%
! ACTCGTGTGCAGCTTTGATG+TGG + Chr8:71344200-71344219 None:intergenic 50.0%
! CCATTTGGAGCTGGTAGAAG+GGG - Chr8:71344337-71344356 MsG0880046269.01.T01:intron 50.0%
! GAATTACCTGGTGGTGCTGA+AGG - Chr8:71344432-71344451 MsG0880046269.01.T01:intron 50.0%
CCCCTTCTACCAGCTCCAAA+TGG + Chr8:71344340-71344359 None:intergenic 55.0%
CTGGTAGAAGGGGTTGTCCT+GGG - Chr8:71344347-71344366 MsG0880046269.01.T01:intron 55.0%
GTCCTGGGCAGTGTATGCTA+CGG - Chr8:71344362-71344381 MsG0880046269.01.T01:intron 55.0%
TCCAATCCTTCAGCACCACC+AGG + Chr8:71344441-71344460 None:intergenic 55.0%
! ACCTGGTGGTGCTGAAGGAT+TGG - Chr8:71344437-71344456 MsG0880046269.01.T01:intron 55.0%
AGCCGTAGCATACACTGCCC+AGG + Chr8:71344367-71344386 None:intergenic 60.0%
GCTGGTAGAAGGGGTTGTCC+TGG - Chr8:71344346-71344365 MsG0880046269.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 71343018 71344536 71343018 ID=MsG0880046269.01;Name=MsG0880046269.01
Chr8 mRNA 71343018 71344536 71343018 ID=MsG0880046269.01.T01;Parent=MsG0880046269.01;Name=MsG0880046269.01.T01;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|1|1|3|0|117
Chr8 exon 71344468 71344536 71344468 ID=MsG0880046269.01.T01:exon:15518;Parent=MsG0880046269.01.T01
Chr8 exon 71343497 71343617 71343497 ID=MsG0880046269.01.T01:exon:15517;Parent=MsG0880046269.01.T01
Chr8 exon 71343018 71343181 71343018 ID=MsG0880046269.01.T01:exon:15516;Parent=MsG0880046269.01.T01
Chr8 CDS 71344468 71344536 71344468 ID=MsG0880046269.01.T01:cds;Parent=MsG0880046269.01.T01
Chr8 CDS 71343497 71343617 71343497 ID=MsG0880046269.01.T01:cds;Parent=MsG0880046269.01.T01
Chr8 CDS 71343018 71343181 71343018 ID=MsG0880046269.01.T01:cds;Parent=MsG0880046269.01.T01
Gene Sequence

>MsG0880046269.01.T01

ATGAGGACTTTGTTGAAGTTTTGCTTTGGATCCAAAGAACAGATCACTTGGCTTTCCTATGACAAAACGGACATGTTTATTGCAGGTACAGACACCATATCCACTTTGCTAGAGTGGTCAATGACAGAACTCTTAAGGCATCCAAATACCATGAAGAAATTGAAAGAAGAAGTAAGTAAAGTGGCTAATGTGTATGCTACGGCTGTCAATGATCTTGTGTTAGCCAACCTAGTACATCAATTTAACTGGGAATTACCTGGTGGTGCTGAAGGATTGGACATGTCTGAATCTTTTGGATTCACAGTTCATAGAAAGATTCCTCTTATGGCAATTGCAACCCCTAACAAGAACTAA

Protein sequence

>MsG0880046269.01.T01

MRTLLKFCFGSKEQITWLSYDKTDMFIAGTDTISTLLEWSMTELLRHPNTMKKLKEEVSKVANVYATAVNDLVLANLVHQFNWELPGGAEGLDMSESFGFTVHRKIPLMAIATPNKN*