AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021853.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGCAACATCATGCTAAA+AGG 0.226951 4:-65194881 MsG0480021853.01.T01:CDS
CAAAGCAGCGTCAAGCTAAA+AGG 0.248224 4:-65194950 MsG0480021853.01.T01:CDS
CTTTCAGCTTGATGATGCTT+TGG 0.336556 4:+65195020 None:intergenic
CACTATGATGATGGATTCGC+TGG 0.395596 4:-65195054 MsG0480021853.01.T01:CDS
TGCTTTGGTAAGACCCCGTC+TGG 0.419898 4:+65194825 None:intergenic
AAAGCAGCGTCAAGCTAAAA+GGG 0.443246 4:-65194949 MsG0480021853.01.T01:CDS
AAAGCAACATCATGCTAAAA+GGG 0.443673 4:-65194880 MsG0480021853.01.T01:CDS
AAAGCATCATCAAGCTGAAA+GGG 0.476885 4:-65195018 MsG0480021853.01.T01:CDS
AGACATTATCACTATGATGA+TGG 0.510577 4:-65195063 MsG0480021853.01.T01:CDS
ATGATGACGATTTGCCAGAC+GGG 0.529560 4:-65194839 MsG0480021853.01.T01:CDS
CAAAGCATCATCAAGCTGAA+AGG 0.530065 4:-65195019 MsG0480021853.01.T01:CDS
GCGAATCCTCGTCATTATCA+TGG 0.530875 4:+65194776 None:intergenic
TGATGAGGATTCACTAGATG+AGG 0.576767 4:-65194979 MsG0480021853.01.T01:CDS
ACATCCTCGCTACGATGATG+AGG 0.614505 4:-65194994 MsG0480021853.01.T01:CDS
TCCTCGTCATTATCATGGTG+AGG 0.625583 4:+65194781 None:intergenic
GATGATGACGATTTGCCAGA+CGG 0.628419 4:-65194840 MsG0480021853.01.T01:CDS
AAGCAACATCATGCTAAAAG+GGG 0.646270 4:-65194879 MsG0480021853.01.T01:CDS
AAGCATCATCAAGCTGAAAG+GGG 0.649585 4:-65195017 MsG0480021853.01.T01:CDS
AAGCAACGACAAGCTAAAAG+CGG 0.652500 4:-65194807 MsG0480021853.01.T01:CDS
TCGTCATCATCGTCATAGTG+AGG 0.664947 4:+65194852 None:intergenic
TGACGAGGATTCGCTACATG+AGG 0.668713 4:-65194767 MsG0480021853.01.T01:CDS
AAGCAGCGTCAAGCTAAAAG+GGG 0.671751 4:-65194948 MsG0480021853.01.T01:CDS
CGACGACGACTCTCTAGATG+AGG 0.674837 4:-65194910 MsG0480021853.01.T01:CDS
TCCTCACCATGATAATGACG+AGG 0.694396 4:-65194782 MsG0480021853.01.T01:CDS
GAATCCTCATCATCGTAGCG+AGG 0.696997 4:+65194990 None:intergenic
TGATGACGATTTGCCAGACG+GGG 0.703928 4:-65194838 MsG0480021853.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGCAACATCATGCTAAAA+GGG - Chr4:65194970-65194989 MsG0480021853.01.T01:CDS 30.0%
AGACATTATCACTATGATGA+TGG - Chr4:65194787-65194806 MsG0480021853.01.T01:CDS 30.0%
AAGCAACATCATGCTAAAAG+GGG - Chr4:65194971-65194990 MsG0480021853.01.T01:CDS 35.0%
CAAAGCAACATCATGCTAAA+AGG - Chr4:65194969-65194988 MsG0480021853.01.T01:CDS 35.0%
! AAAGCATCATCAAGCTGAAA+GGG - Chr4:65194832-65194851 MsG0480021853.01.T01:CDS 35.0%
! TCTTGTGAGCTTGATGTTTT+TGG + Chr4:65194768-65194787 None:intergenic 35.0%
AAAGCAGCGTCAAGCTAAAA+GGG - Chr4:65194901-65194920 MsG0480021853.01.T01:CDS 40.0%
AAGCAACGACAAGCTAAAAG+CGG - Chr4:65195043-65195062 MsG0480021853.01.T01:CDS 40.0%
AAGCATCATCAAGCTGAAAG+GGG - Chr4:65194833-65194852 MsG0480021853.01.T01:CDS 40.0%
TGATGAGGATTCACTAGATG+AGG - Chr4:65194871-65194890 MsG0480021853.01.T01:CDS 40.0%
! CAAAGCATCATCAAGCTGAA+AGG - Chr4:65194831-65194850 MsG0480021853.01.T01:CDS 40.0%
!! CTTTCAGCTTGATGATGCTT+TGG + Chr4:65194833-65194852 None:intergenic 40.0%
!!! CTTTTAGCTTGTCGTTGCTT+TGG + Chr4:65195043-65195062 None:intergenic 40.0%
AAGCAGCGTCAAGCTAAAAG+GGG - Chr4:65194902-65194921 MsG0480021853.01.T01:CDS 45.0%
CAAAGCAGCGTCAAGCTAAA+AGG - Chr4:65194900-65194919 MsG0480021853.01.T01:CDS 45.0%
GCGAATCCTCGTCATTATCA+TGG + Chr4:65195077-65195096 None:intergenic 45.0%
TCCTCGTCATTATCATGGTG+AGG + Chr4:65195072-65195091 None:intergenic 45.0%
TCGTCATCATCGTCATAGTG+AGG + Chr4:65195001-65195020 None:intergenic 45.0%
! ATGATGACGATTTGCCAGAC+GGG - Chr4:65195011-65195030 MsG0480021853.01.T01:CDS 45.0%
! GATGATGACGATTTGCCAGA+CGG - Chr4:65195010-65195029 MsG0480021853.01.T01:CDS 45.0%
! TCCTCACCATGATAATGACG+AGG - Chr4:65195068-65195087 MsG0480021853.01.T01:CDS 45.0%
!! CACTATGATGATGGATTCGC+TGG - Chr4:65194796-65194815 MsG0480021853.01.T01:CDS 45.0%
!!! CTTTTAGCTTGACGCTGCTT+TGG + Chr4:65194902-65194921 None:intergenic 45.0%
!!! GAGCTTGATGTTTTTGGCGA+CGG + Chr4:65194762-65194781 None:intergenic 45.0%
!!! GATGTTTTTGGCGACGGTTT+GGG + Chr4:65194756-65194775 None:intergenic 45.0%
!!! TGATGTTTTTGGCGACGGTT+TGG + Chr4:65194757-65194776 None:intergenic 45.0%
ACATCCTCGCTACGATGATG+AGG - Chr4:65194856-65194875 MsG0480021853.01.T01:CDS 50.0%
GAATCCTCATCATCGTAGCG+AGG + Chr4:65194863-65194882 None:intergenic 50.0%
TGACGAGGATTCGCTACATG+AGG - Chr4:65195083-65195102 MsG0480021853.01.T01:CDS 50.0%
! TGATGACGATTTGCCAGACG+GGG - Chr4:65195012-65195031 MsG0480021853.01.T01:CDS 50.0%
CGACGACGACTCTCTAGATG+AGG - Chr4:65194940-65194959 MsG0480021853.01.T01:CDS 55.0%
TGCTTTGGTAAGACCCCGTC+TGG + Chr4:65195028-65195047 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65194743 65195129 65194743 ID=MsG0480021853.01;Name=MsG0480021853.01
Chr4 mRNA 65194743 65195129 65194743 ID=MsG0480021853.01.T01;Parent=MsG0480021853.01;Name=MsG0480021853.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|128
Chr4 exon 65194743 65195129 65194743 ID=MsG0480021853.01.T01:exon:4705;Parent=MsG0480021853.01.T01
Chr4 CDS 65194743 65195129 65194743 ID=MsG0480021853.01.T01:cds;Parent=MsG0480021853.01.T01
Gene Sequence

>MsG0480021853.01.T01

ATGAGATCTTCCCAAACCGTCGCCAAAAACATCAAGCTCACAAGAGACATTATCACTATGATGATGGATTCGCTGGATTCACTCTTACCAAAGCATCATCAAGCTGAAAGGGGACATCCTCGCTACGATGATGAGGATTCACTAGATGAGGTTTTACCAAAGCAGCGTCAAGCTAAAAGGGGTCGTTCTCACTATGACGACGACGACTCTCTAGATGAGGTCTTAGCAAAGCAACATCATGCTAAAAGGGGACATCCTCACTATGACGATGATGACGATTTGCCAGACGGGGTCTTACCAAAGCAACGACAAGCTAAAAGCGGCATCCTCACCATGATAATGACGAGGATTCGCTACATGAGGACTTACCAAGAATCATCAAGCTAA

Protein sequence

>MsG0480021853.01.T01

MRSSQTVAKNIKLTRDIITMMMDSLDSLLPKHHQAERGHPRYDDEDSLDEVLPKQRQAKRGRSHYDDDDSLDEVLAKQHHAKRGHPHYDDDDDLPDGVLPKQRQAKSGILTMIMTRIRYMRTYQESSS*