AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040596.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040596.01.T01 MTR_4g126750 86.538 52 7 0 1 52 101 152 1.94e-27 95.9
MsG0780040596.01.T01 MTR_1g020070 84.615 52 8 0 1 52 177 228 1.89e-26 95.5
MsG0780040596.01.T01 MTR_8g107280 85.106 47 7 0 1 47 547 593 4.46e-22 87.0
MsG0780040596.01.T01 MTR_4g094205 59.574 47 19 0 1 47 556 602 7.91e-13 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040596.01.T01 AT3G17970 61.702 47 18 0 1 47 542 588 6.93e-14 63.9
MsG0780040596.01.T01 AT5G09420 58.333 48 20 0 1 48 556 603 2.80e-11 56.2

Find 9 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCTAGTTGTACCTCTA+TGG 0.346394 7:+79726034 None:intergenic
AGGTACAACTAGAGAGATAC+TGG 0.350120 7:-79726028 MsG0780040596.01.T01:CDS
TAATACTCAAGAAGAGTTGA+AGG 0.447543 7:-79725909 None:intergenic
TGTTGGTTGGCTCAAGTACA+AGG 0.448590 7:+79725968 None:intergenic
CTTCAGCTTCTGAAAGATTG+AGG 0.458231 7:-79725936 MsG0780040596.01.T01:CDS
TACTTGAGCCAACCAACAAA+AGG 0.509714 7:-79725963 MsG0780040596.01.T01:CDS
ATGAAGGCATACTTCCATAG+AGG 0.619428 7:-79726048 MsG0780040596.01.T01:CDS
GTTGGTTGGCTCAAGTACAA+GGG 0.632408 7:+79725969 None:intergenic
GGTACAACTAGAGAGATACT+GGG 0.665492 7:-79726027 MsG0780040596.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGTACAACTAGAGAGATAC+TGG - Chr7:79725932-79725951 MsG0780040596.01.T01:CDS 40.0%
GGTACAACTAGAGAGATACT+GGG - Chr7:79725933-79725952 MsG0780040596.01.T01:CDS 40.0%
TACTTGAGCCAACCAACAAA+AGG - Chr7:79725997-79726016 MsG0780040596.01.T01:CDS 40.0%
TCTCTCTAGTTGTACCTCTA+TGG + Chr7:79725929-79725948 None:intergenic 40.0%
! ATGAAGGCATACTTCCATAG+AGG - Chr7:79725912-79725931 MsG0780040596.01.T01:CDS 40.0%
! CTTCAGCTTCTGAAAGATTG+AGG - Chr7:79726024-79726043 MsG0780040596.01.T01:CDS 40.0%
! AGCTGAAGCAGACCTTTTGT+TGG + Chr7:79726012-79726031 None:intergenic 45.0%
! GAAGCAGACCTTTTGTTGGT+TGG + Chr7:79726008-79726027 None:intergenic 45.0%
! GTTGGTTGGCTCAAGTACAA+GGG + Chr7:79725994-79726013 None:intergenic 45.0%
! TGTTGGTTGGCTCAAGTACA+AGG + Chr7:79725995-79726014 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79725912 79726070 79725912 ID=MsG0780040596.01;Name=MsG0780040596.01
Chr7 mRNA 79725912 79726070 79725912 ID=MsG0780040596.01.T01;Parent=MsG0780040596.01;Name=MsG0780040596.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|0|1|0|52
Chr7 exon 79725912 79726070 79725912 ID=MsG0780040596.01.T01:exon:2027;Parent=MsG0780040596.01.T01
Chr7 CDS 79725912 79726070 79725912 ID=MsG0780040596.01.T01:cds;Parent=MsG0780040596.01.T01
Gene Sequence

>MsG0780040596.01.T01

ATGAAGGCATACTTCCATAGAGGTACAACTAGAGAGATACTGGGCTACTACAAAGAAGCAATTGATGATTTTAAATATGCCCTTGTACTTGAGCCAACCAACAAAAGGTCTGCTTCAGCTTCTGAAAGATTGAGGAATCTAATACTCAAGAAGAGTTGA

Protein sequence

>MsG0780040596.01.T01

MKAYFHRGTTREILGYYKEAIDDFKYALVLEPTNKRSASASERLRNLILKKS*