AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0000100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0000100 MtrunA17_Chr1g0155331 97.951 244 5 0 1 244 1 244 1.78e-178 489
Msa0000100 MtrunA17_Chr2g0298841 98.361 244 4 0 1 244 1 244 3.01e-178 489
Msa0000100 MtrunA17_Chr4g0002321 86.722 241 32 0 4 244 3 243 6.72e-159 440
Msa0000100 MtrunA17_Chr7g0237701 45.749 247 130 2 1 244 1 246 1.94e-73 223
Msa0000100 MtrunA17_Chr1g0155311 98.361 61 1 0 174 234 2 62 3.29e-39 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0000100 AT3G13580.5 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.4 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.2 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.3 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.1 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.7 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.8 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.6 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT3G13580.9 82.377 244 43 0 1 244 1 244 2.61e-153 426
Msa0000100 AT2G01250.1 84.583 240 37 0 5 244 3 242 6.89e-152 422
Msa0000100 AT2G44120.2 83.333 240 40 0 5 244 8 247 2.47e-150 419
Msa0000100 AT2G44120.1 83.333 240 40 0 5 244 3 242 2.86e-150 418
Msa0000100 AT2G01250.2 80.000 175 35 0 5 179 3 177 5.40e-97 281
Msa0000100 AT1G80750.1 43.320 247 137 2 1 244 1 247 1.95e-63 198

Find 85 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATCTTCCTTGATTT+TGG 0.006132 1_1:+757941 None:intergenic
TTCTCTGACACTCTTCAAAT+TGG 0.182246 1_1:+756849 None:intergenic
TGCTTCTTCAAAACAGATTC+AGG 0.223629 1_1:+758536 None:intergenic
AATATTCAATTGCAGGTTCT+TGG 0.263781 1_1:-756474 Msa0000100:intron
AAGGGAGGAGTAATTTATTA+AGG 0.269195 1_1:-755856 Msa0000100:three_prime_UTR
ACTCATTAGAAGGATGAATT+AGG 0.270598 1_1:-756265 Msa0000100:exon
TACAATTGTTAGAATCTATA+TGG 0.271097 1_1:-756015 Msa0000100:three_prime_UTR
GTGTCGTTTAAGGTTGATTA+TGG 0.274625 1_1:-756233 Msa0000100:three_prime_UTR
CTGAAACCAGGAAGCTTATC+TGG 0.274950 1_1:-758448 Msa0000100:CDS
TAGAAGGATGAATTAGGTTT+TGG 0.280297 1_1:-756259 Msa0000100:exon
TTTCAAGCTCAAAGCTCCTT+TGG 0.284641 1_1:-756361 Msa0000100:CDS
GTGGTTTCTTAACTAAAATA+AGG 0.289087 1_1:-755920 Msa0000100:three_prime_UTR
TTCAAGCTCAAAGCTCCTTT+GGG 0.329811 1_1:-756360 Msa0000100:CDS
TTGATCTACAAGCGTGGTTA+TGG 0.334090 1_1:-756826 Msa0000100:CDS
TTCAGCAAAAGGAGTTGATT+AGG 0.371855 1_1:-758296 Msa0000100:intron
AAAACTTTCACTGCACTTGC+AGG 0.373710 1_1:+756112 None:intergenic
TCTACTCCAGATAAGCTTCC+TGG 0.375959 1_1:+758442 None:intergenic
CGGTTGCTTTGTTCACTTTG+AGG 0.382967 1_1:+757007 None:intergenic
AACACTTGAACTCAACATAA+AGG 0.387036 1_1:-755875 Msa0000100:three_prime_UTR
AAGGTGGTGATGCTGGTAAC+AGG 0.401808 1_1:-756305 Msa0000100:CDS
ATATTCAATTGCAGGTTCTT+GGG 0.423006 1_1:-756473 Msa0000100:intron
ACACTTGAACTCAACATAAA+GGG 0.426348 1_1:-755874 Msa0000100:three_prime_UTR
GTACTCCTTAGCGTACTGTT+TGG 0.434466 1_1:+758418 None:intergenic
TGGTTTCTTAACTAAAATAA+GGG 0.436696 1_1:-755919 Msa0000100:three_prime_UTR
ATGGGCATTGGTGAAGAAAC+AGG 0.439903 1_1:-758498 Msa0000100:CDS
ACAACATCGATTCACTGAAA+TGG 0.441842 1_1:-758585 Msa0000100:exon
ACATCAATGAACTCATTAGA+AGG 0.442744 1_1:-756275 Msa0000100:CDS
CTTATTTGTCAATGATGACT+TGG 0.447185 1_1:-756087 Msa0000100:three_prime_UTR
TGAAGGTGAACGGCGCTTCA+AGG 0.448474 1_1:+758626 None:intergenic
GCAGCTTCTGCGATTGAGAC+AGG 0.461345 1_1:-757921 Msa0000100:intron
GTTGAGCCATATGTCACCTA+TGG 0.461514 1_1:-756967 Msa0000100:CDS
TCTCTGACACTCTTCAAATT+GGG 0.464078 1_1:+756850 None:intergenic
GAAGCGTGAAGCTAAGCTTA+AGG 0.464202 1_1:-758271 Msa0000100:CDS
AAGCGTGAAGCTAAGCTTAA+GGG 0.467088 1_1:-758270 Msa0000100:CDS
ATGAACAAAAGCTTAGCTTC+AGG 0.472114 1_1:+758231 None:intergenic
AGCAGAAAAGGAACGAGGAA+TGG 0.472482 1_1:-758517 Msa0000100:CDS
GCATACCCATAGGTGACATA+TGG 0.482193 1_1:+756961 None:intergenic
AGATAACTCTATCATCGAAC+AGG 0.483740 1_1:-756773 Msa0000100:intron
AAGAGAAATCACTATGTTGA+AGG 0.489111 1_1:-756324 Msa0000100:CDS
AGAAGAAGAGAGCTGAAACC+AGG 0.494410 1_1:-758460 Msa0000100:CDS
CAACCGTGAATATGCTCCAC+AGG 0.496550 1_1:-756990 Msa0000100:CDS
TAATTTCAGTATCAATGCAA+TGG 0.497010 1_1:-757966 Msa0000100:intron
AAAGGAGCTTTGAGCTTGAA+AGG 0.504831 1_1:+756363 None:intergenic
GAGAGGCAAACAACTTCCTA+TGG 0.504947 1_1:-756386 Msa0000100:CDS
TCTCTGAGTGAAAGTGAAGC+AGG 0.506218 1_1:-755990 Msa0000100:three_prime_UTR
AACCCTGTGGAGCATATTCA+CGG 0.507599 1_1:+756987 None:intergenic
TTCTGTGTTAATATTTGTGA+TGG 0.508992 1_1:-755950 Msa0000100:three_prime_UTR
TATTTGTGATGGGAGAATAG+TGG 0.511227 1_1:-755939 Msa0000100:three_prime_UTR
ATGCAATGGACCCAAAATCA+AGG 0.518414 1_1:-757952 Msa0000100:CDS
GCAGAAAAGGAACGAGGAAT+GGG 0.522675 1_1:-758516 Msa0000100:CDS
TGAAGTGAGGTCCAACAGTA+AGG 0.528046 1_1:+756409 None:intergenic
AAAGTGAAGCAGGGAATGTA+TGG 0.531319 1_1:-755980 Msa0000100:three_prime_UTR
TATGTTGAAGGTGGTGATGC+TGG 0.533299 1_1:-756312 Msa0000100:CDS
GTTGTGGTTCTGTTCTGTGA+AGG 0.534006 1_1:+758609 None:intergenic
ATTCATGAGATCCTTACTGT+TGG 0.542331 1_1:-756420 Msa0000100:CDS
GTGGTTATGGAAAGGTTGAT+AGG 0.547437 1_1:-756813 Msa0000100:CDS
TTGCAGGTTCTTGGGAAACA+TGG 0.547799 1_1:-756465 Msa0000100:intron
TTCTGTTCTGTGAAGGTGAA+CGG 0.551457 1_1:+758616 None:intergenic
GTGAATCGATGTTGTTGTTG+TGG 0.553156 1_1:+758593 None:intergenic
TTCTTCTTCATGCCACCCAA+AGG 0.554983 1_1:+756345 None:intergenic
CGCTAAGGAGTACGATGACT+CGG 0.560110 1_1:-758408 Msa0000100:intron
TTGTTGTTTGCATTCACGAA+AGG 0.561345 1_1:-756051 Msa0000100:three_prime_UTR
CTTTGAGCTTGAAAGGCCAT+AGG 0.568033 1_1:+756370 None:intergenic
TTGTTCATTATCAGGATCAG+AGG 0.568101 1_1:-758216 Msa0000100:CDS
AAGCTCAAAGCTCCTTTGGG+TGG 0.570830 1_1:-756357 Msa0000100:CDS
TTGTTTGCCTCTCTGAAGTG+AGG 0.573961 1_1:+756396 None:intergenic
TAGAGCCAAACAGTACGCTA+AGG 0.574389 1_1:-758423 Msa0000100:CDS
AAGGAACGAGGAATGGGCAT+TGG 0.574615 1_1:-758510 Msa0000100:CDS
CAAAACAGATTCAGGAACAA+CGG 0.575093 1_1:+758544 None:intergenic
TGTTCATTATCAGGATCAGA+GGG 0.584717 1_1:-758215 Msa0000100:intron
TTGAGCCATATGTCACCTAT+GGG 0.595042 1_1:-756966 Msa0000100:intron
TCTGTGTTAATATTTGTGAT+GGG 0.595084 1_1:-755949 Msa0000100:three_prime_UTR
ATTCACTGAAATGGCCAAAG+AGG 0.608425 1_1:-758576 Msa0000100:exon
CTCTGAGTGAAAGTGAAGCA+GGG 0.610321 1_1:-755989 Msa0000100:three_prime_UTR
CTACAAGCGTGGTTATGGAA+AGG 0.617075 1_1:-756821 Msa0000100:CDS
CGTGAAGCTAAGCTTAAGGG+TGG 0.634387 1_1:-758267 Msa0000100:CDS
AGAAATCACTATGTTGAAGG+TGG 0.637998 1_1:-756321 Msa0000100:CDS
AGGTGGTGATGCTGGTAACA+GGG 0.651466 1_1:-756304 Msa0000100:CDS
TGACATATGGCTCAACCCTG+TGG 0.662121 1_1:+756974 None:intergenic
CTTGAACTCAACATAAAGGG+AGG 0.665267 1_1:-755871 Msa0000100:three_prime_UTR
AACCGTGAATATGCTCCACA+GGG 0.683451 1_1:-756989 Msa0000100:CDS
GAAGAAGCAGAAAAGGAACG+AGG 0.689511 1_1:-758522 Msa0000100:CDS
AGAGAATTGATCTACAAGCG+TGG 0.711706 1_1:-756832 Msa0000100:CDS
TGTTGGACCTCACTTCAGAG+AGG 0.727556 1_1:-756403 Msa0000100:CDS
TGGTGATGCTGGTAACAGGG+AGG 0.759776 1_1:-756301 Msa0000100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATAATTTTGTTCTGTTT+TGG - chr1_1:756331-756350 Msa0000100:CDS 15.0%
!! ATTCTGATTATAACTTTAAA+CGG + chr1_1:756644-756663 None:intergenic 15.0%
!!! AAAGGAAAAAAAAGTTTTTT+TGG + chr1_1:756966-756985 None:intergenic 15.0%
!!! TTTTTTTTAGTTTAGAGATA+AGG - chr1_1:757245-757264 Msa0000100:intron 15.0%
!! AAAAATTAAAGAGAAATACT+AGG + chr1_1:757292-757311 None:intergenic 15.0%
!!! TAGTATTTCTCTTTAATTTT+TGG - chr1_1:757291-757310 Msa0000100:intron 15.0%
!!! AAAATCTTTAAAAACAAAAC+TGG + chr1_1:758256-758275 None:intergenic 15.0%
!! AAAAATTAGTATGTTTGTAT+TGG - chr1_1:758607-758626 Msa0000100:five_prime_UTR 15.0%
!!! ACAATATCTTGATTTTTTAA+AGG - chr1_1:758635-758654 Msa0000100:five_prime_UTR 15.0%
!! TGTATTAATAATTCAGATAG+AGG + chr1_1:756915-756934 None:intergenic 20.0%
!!! TTTCCTTTTTTGTATTGTTT+AGG - chr1_1:756978-756997 Msa0000100:CDS 20.0%
!! ATCTTCAATAGTTAAGTAAA+AGG + chr1_1:757145-757164 None:intergenic 20.0%
!!! GATTCTAACTTTGAAAATTT+GGG + chr1_1:757797-757816 None:intergenic 20.0%
!!! AGATTCTAACTTTGAAAATT+TGG + chr1_1:757798-757817 None:intergenic 20.0%
!! GCAAAATAGAAAAAAATAAC+TGG + chr1_1:757933-757952 None:intergenic 20.0%
!! TACAATTGTTAGAATCTATA+TGG - chr1_1:758421-758440 Msa0000100:CDS 20.0%
!! TGGTTTCTTAACTAAAATAA+GGG - chr1_1:758517-758536 Msa0000100:CDS 20.0%
! TAATTTCAGTATCAATGCAA+TGG - chr1_1:756470-756489 Msa0000100:intron 25.0%
! AAGGGATCAATAGTTATTAT+AGG - chr1_1:756770-756789 Msa0000100:intron 25.0%
! ATAATTGAGCTTTCATCTTA+TGG - chr1_1:756821-756840 Msa0000100:CDS 25.0%
! ACATGAAAATAACCAATTGT+TGG + chr1_1:756857-756876 None:intergenic 25.0%
! TAAGTAAAAGGTTATCATAC+AGG + chr1_1:757133-757152 None:intergenic 25.0%
!! GCTTATTCTTCTATTTGTTT+TGG - chr1_1:757383-757402 Msa0000100:intron 25.0%
! TAACTCATTATAACTCTGTA+TGG - chr1_1:757528-757547 Msa0000100:intron 25.0%
! AACTCATTATAACTCTGTAT+GGG - chr1_1:757529-757548 Msa0000100:intron 25.0%
! TAAAATACAAAGTCAGAATG+AGG + chr1_1:757774-757793 None:intergenic 25.0%
! TTCTGTGTTAATATTTGTGA+TGG - chr1_1:758486-758505 Msa0000100:CDS 25.0%
! TCTGTGTTAATATTTGTGAT+GGG - chr1_1:758487-758506 Msa0000100:CDS 25.0%
! GTGGTTTCTTAACTAAAATA+AGG - chr1_1:758516-758535 Msa0000100:CDS 25.0%
AATTTGTGTGTTTCAGCAAA+AGG - chr1_1:756129-756148 Msa0000100:three_prime_UTR 30.0%
! CTAAGCTTTTGTTCATTATC+AGG - chr1_1:756212-756231 Msa0000100:three_prime_UTR 30.0%
!!! CTGTTTTGGAAAAATGATAG+AGG - chr1_1:756345-756364 Msa0000100:CDS 30.0%
TGAATAAAATGTATCCTGTG+TGG - chr1_1:756396-756415 Msa0000100:CDS 30.0%
GAATAAAATGTATCCTGTGT+GGG - chr1_1:756397-756416 Msa0000100:CDS 30.0%
! GCAAAATCTTCCTTGATTTT+GGG + chr1_1:756497-756516 None:intergenic 30.0%
! TGCAAAATCTTCCTTGATTT+TGG + chr1_1:756498-756517 None:intergenic 30.0%
!!! CAATTGGTTTTGTTCTTGAT+GGG - chr1_1:756563-756582 Msa0000100:intron 30.0%
! TTTCAAAGTGCCTTCAATTA+AGG - chr1_1:756751-756770 Msa0000100:intron 30.0%
! TTCAAAGTGCCTTCAATTAA+GGG - chr1_1:756752-756771 Msa0000100:intron 30.0%
ACTATTGATCCCTTAATTGA+AGG + chr1_1:756764-756783 None:intergenic 30.0%
! TAATTTTGCGGTGATTATGT+TGG + chr1_1:756890-756909 None:intergenic 30.0%
CAGCCTAAACAATACAAAAA+AGG + chr1_1:756984-757003 None:intergenic 30.0%
GCTGTTCATCTATATAAGAA+TGG - chr1_1:757000-757019 Msa0000100:CDS 30.0%
CTAATTATTTGATACTGAGC+AGG - chr1_1:757102-757121 Msa0000100:intron 30.0%
CTAAACCAGTTGGTTATAAT+GGG + chr1_1:757182-757201 None:intergenic 30.0%
ACTAAACCAGTTGGTTATAA+TGG + chr1_1:757183-757202 None:intergenic 30.0%
AAAATTGACAATGACAAGGA+TGG + chr1_1:757209-757228 None:intergenic 30.0%
TCAGAAAATTGACAATGACA+AGG + chr1_1:757213-757232 None:intergenic 30.0%
! TTTAGTTTAGAGATAAGGTG+TGG - chr1_1:757250-757269 Msa0000100:intron 30.0%
!!! TCTCTTTAATTTTTGGCTTC+TGG - chr1_1:757298-757317 Msa0000100:intron 30.0%
!! GGTGCTATTATTATTGTTGA+AGG - chr1_1:757899-757918 Msa0000100:intron 30.0%
CTGACATAATATTCAATTGC+AGG - chr1_1:757955-757974 Msa0000100:CDS 30.0%
AATATTCAATTGCAGGTTCT+TGG - chr1_1:757962-757981 Msa0000100:intron 30.0%
ATATTCAATTGCAGGTTCTT+GGG - chr1_1:757963-757982 Msa0000100:intron 30.0%
AAGAGAAATCACTATGTTGA+AGG - chr1_1:758112-758131 Msa0000100:intron 30.0%
ACATCAATGAACTCATTAGA+AGG - chr1_1:758161-758180 Msa0000100:intron 30.0%
ACTCATTAGAAGGATGAATT+AGG - chr1_1:758171-758190 Msa0000100:intron 30.0%
! TAGAAGGATGAATTAGGTTT+TGG - chr1_1:758177-758196 Msa0000100:intron 30.0%
CTTATTTGTCAATGATGACT+TGG - chr1_1:758349-758368 Msa0000100:intron 30.0%
AACACTTGAACTCAACATAA+AGG - chr1_1:758561-758580 Msa0000100:CDS 30.0%
ACACTTGAACTCAACATAAA+GGG - chr1_1:758562-758581 Msa0000100:CDS 30.0%
AAGGGAGGAGTAATTTATTA+AGG - chr1_1:758580-758599 Msa0000100:exon 30.0%
!! ACAACATCGATTCACTGAAA+TGG - chr1_1:755851-755870 Msa0000100:three_prime_UTR 35.0%
CAAAACAGATTCAGGAACAA+CGG + chr1_1:755895-755914 None:intergenic 35.0%
TGCTTCTTCAAAACAGATTC+AGG + chr1_1:755903-755922 None:intergenic 35.0%
!!! CTGTTTTGAAGAAGCAGAAA+AGG - chr1_1:755907-755926 Msa0000100:three_prime_UTR 35.0%
! TTCAGCAAAAGGAGTTGATT+AGG - chr1_1:756140-756159 Msa0000100:three_prime_UTR 35.0%
ATGAACAAAAGCTTAGCTTC+AGG + chr1_1:756208-756227 None:intergenic 35.0%
TTGTTCATTATCAGGATCAG+AGG - chr1_1:756220-756239 Msa0000100:three_prime_UTR 35.0%
TGTTCATTATCAGGATCAGA+GGG - chr1_1:756221-756240 Msa0000100:three_prime_UTR 35.0%
CAAATAAGAATAACCCACAC+AGG + chr1_1:756413-756432 None:intergenic 35.0%
TTGAGACAGGTAAATCAGAT+TGG - chr1_1:756528-756547 Msa0000100:intron 35.0%
ACAGGTAAATCAGATTGGAT+TGG - chr1_1:756533-756552 Msa0000100:intron 35.0%
!! TTGGATTGGTTTGTTGCAAT+TGG - chr1_1:756547-756566 Msa0000100:intron 35.0%
!!! GCAATTGGTTTTGTTCTTGA+TGG - chr1_1:756562-756581 Msa0000100:intron 35.0%
!! TTTTGTTCTTGATGGGTGTT+TGG - chr1_1:756570-756589 Msa0000100:intron 35.0%
! TTAGTTGATGTATTTGTCGC+TGG - chr1_1:756607-756626 Msa0000100:intron 35.0%
TGATTGCACTTTGCAATTGT+TGG - chr1_1:756713-756732 Msa0000100:intron 35.0%
GATTGCACTTTGCAATTGTT+GGG - chr1_1:756714-756733 Msa0000100:intron 35.0%
! CAGATAGAGGAGTAATTTTG+CGG + chr1_1:756902-756921 None:intergenic 35.0%
ATTCACCCATTATAACCAAC+TGG - chr1_1:757174-757193 Msa0000100:intron 35.0%
ATACTCGTATCTTCTAGACT+CGG - chr1_1:757342-757361 Msa0000100:intron 35.0%
TCTCTGACACTCTTCAAATT+GGG + chr1_1:757589-757608 None:intergenic 35.0%
TTCTCTGACACTCTTCAAAT+TGG + chr1_1:757590-757609 None:intergenic 35.0%
AGATAACTCTATCATCGAAC+AGG - chr1_1:757663-757682 Msa0000100:intron 35.0%
TGTGTAATCGAATGAAAGCA+GGG + chr1_1:757712-757731 None:intergenic 35.0%
AATAATAGCACCACCATACA+CGG + chr1_1:757891-757910 None:intergenic 35.0%
ATTCATGAGATCCTTACTGT+TGG - chr1_1:758016-758035 Msa0000100:intron 35.0%
AGAAATCACTATGTTGAAGG+TGG - chr1_1:758115-758134 Msa0000100:intron 35.0%
!! GTGTCGTTTAAGGTTGATTA+TGG - chr1_1:758203-758222 Msa0000100:intron 35.0%
!! TTGTTGTTTGCATTCACGAA+AGG - chr1_1:758385-758404 Msa0000100:intron 35.0%
! TATTTGTGATGGGAGAATAG+TGG - chr1_1:758497-758516 Msa0000100:CDS 35.0%
TTCTGTTCTGTGAAGGTGAA+CGG + chr1_1:755823-755842 None:intergenic 40.0%
! GTGAATCGATGTTGTTGTTG+TGG + chr1_1:755846-755865 None:intergenic 40.0%
ATTCACTGAAATGGCCAAAG+AGG - chr1_1:755860-755879 Msa0000100:three_prime_UTR 40.0%
! AACGGTTTTCACTTCCTCTT+TGG + chr1_1:755877-755896 None:intergenic 40.0%
AAGCGTGAAGCTAAGCTTAA+GGG - chr1_1:756166-756185 Msa0000100:three_prime_UTR 40.0%
ATGCAATGGACCCAAAATCA+AGG - chr1_1:756484-756503 Msa0000100:intron 40.0%
TCGGTGCAATTTATCAGTGT+AGG - chr1_1:757361-757380 Msa0000100:intron 40.0%
!! TTTTGGCTGCAGATCTTCAA+TGG - chr1_1:757400-757419 Msa0000100:intron 40.0%
TTGAGCCATATGTCACCTAT+GGG - chr1_1:757470-757489 Msa0000100:intron 40.0%
TTGAGATTCTGCATACCCAT+AGG + chr1_1:757488-757507 None:intergenic 40.0%
AGAGAATTGATCTACAAGCG+TGG - chr1_1:757604-757623 Msa0000100:intron 40.0%
TTGATCTACAAGCGTGGTTA+TGG - chr1_1:757610-757629 Msa0000100:intron 40.0%
! GTGGTTATGGAAAGGTTGAT+AGG - chr1_1:757623-757642 Msa0000100:intron 40.0%
CTGTGTAATCGAATGAAAGC+AGG + chr1_1:757713-757732 None:intergenic 40.0%
AATGAGGAGAGATCCTATCT+AGG + chr1_1:757758-757777 None:intergenic 40.0%
TTAGACAAAGTCACGCAATC+TGG + chr1_1:757847-757866 None:intergenic 40.0%
ATAATAGCACCACCATACAC+GGG + chr1_1:757890-757909 None:intergenic 40.0%
!! AAAGGAGCTTTGAGCTTGAA+AGG + chr1_1:758076-758095 None:intergenic 40.0%
TTTCAAGCTCAAAGCTCCTT+TGG - chr1_1:758075-758094 Msa0000100:intron 40.0%
TTCAAGCTCAAAGCTCCTTT+GGG - chr1_1:758076-758095 Msa0000100:intron 40.0%
!!! GTTTTGGCATGTGTCGTTTA+AGG - chr1_1:758193-758212 Msa0000100:intron 40.0%
AAAACTTTCACTGCACTTGC+AGG + chr1_1:758327-758346 None:intergenic 40.0%
AAAGTGAAGCAGGGAATGTA+TGG - chr1_1:758456-758475 Msa0000100:CDS 40.0%
CTTGAACTCAACATAAAGGG+AGG - chr1_1:758565-758584 Msa0000100:CDS 40.0%
! GTTGTGGTTCTGTTCTGTGA+AGG + chr1_1:755830-755849 None:intergenic 45.0%
GAAGAAGCAGAAAAGGAACG+AGG - chr1_1:755914-755933 Msa0000100:three_prime_UTR 45.0%
AGCAGAAAAGGAACGAGGAA+TGG - chr1_1:755919-755938 Msa0000100:three_prime_UTR 45.0%
GCAGAAAAGGAACGAGGAAT+GGG - chr1_1:755920-755939 Msa0000100:three_prime_UTR 45.0%
!! ATGGGCATTGGTGAAGAAAC+AGG - chr1_1:755938-755957 Msa0000100:three_prime_UTR 45.0%
AGAAGAAGAGAGCTGAAACC+AGG - chr1_1:755976-755995 Msa0000100:three_prime_UTR 45.0%
CTGAAACCAGGAAGCTTATC+TGG - chr1_1:755988-756007 Msa0000100:three_prime_UTR 45.0%
TCTACTCCAGATAAGCTTCC+TGG + chr1_1:755997-756016 None:intergenic 45.0%
TAGAGCCAAACAGTACGCTA+AGG - chr1_1:756013-756032 Msa0000100:three_prime_UTR 45.0%
GTACTCCTTAGCGTACTGTT+TGG + chr1_1:756021-756040 None:intergenic 45.0%
GAAGCGTGAAGCTAAGCTTA+AGG - chr1_1:756165-756184 Msa0000100:three_prime_UTR 45.0%
CTTTGCAATTGTTGGGTGCT+TGG - chr1_1:756721-756740 Msa0000100:intron 45.0%
GGCTAAGTGTCTCCAACAAT+TGG - chr1_1:756842-756861 Msa0000100:CDS 45.0%
TTTGCGGTGATTATGTTGGC+AGG + chr1_1:756886-756905 None:intergenic 45.0%
GGATGGAGAACTAAACCAGT+TGG + chr1_1:757192-757211 None:intergenic 45.0%
! CGGTTGCTTTGTTCACTTTG+AGG + chr1_1:757432-757451 None:intergenic 45.0%
AACCGTGAATATGCTCCACA+GGG - chr1_1:757447-757466 Msa0000100:intron 45.0%
AACCCTGTGGAGCATATTCA+CGG + chr1_1:757452-757471 None:intergenic 45.0%
GTTGAGCCATATGTCACCTA+TGG - chr1_1:757469-757488 Msa0000100:intron 45.0%
GCATACCCATAGGTGACATA+TGG + chr1_1:757478-757497 None:intergenic 45.0%
CTACAAGCGTGGTTATGGAA+AGG - chr1_1:757615-757634 Msa0000100:intron 45.0%
AGGAGAGATCCTATCTAGGT+CGG + chr1_1:757754-757773 None:intergenic 45.0%
TCTGGACTGTCTCATCAAGT+TGG + chr1_1:757829-757848 None:intergenic 45.0%
TTGCAGGTTCTTGGGAAACA+TGG - chr1_1:757971-757990 Msa0000100:intron 45.0%
TGAAGTGAGGTCCAACAGTA+AGG + chr1_1:758030-758049 None:intergenic 45.0%
!! TTGTTTGCCTCTCTGAAGTG+AGG + chr1_1:758043-758062 None:intergenic 45.0%
GAGAGGCAAACAACTTCCTA+TGG - chr1_1:758050-758069 Msa0000100:intron 45.0%
CTTTGAGCTTGAAAGGCCAT+AGG + chr1_1:758069-758088 None:intergenic 45.0%
TTCTTCTTCATGCCACCCAA+AGG + chr1_1:758094-758113 None:intergenic 45.0%
! TATGTTGAAGGTGGTGATGC+TGG - chr1_1:758124-758143 Msa0000100:intron 45.0%
TCTCTGAGTGAAAGTGAAGC+AGG - chr1_1:758446-758465 Msa0000100:CDS 45.0%
CTCTGAGTGAAAGTGAAGCA+GGG - chr1_1:758447-758466 Msa0000100:CDS 45.0%
!!! TAAAAAAATATGTTTTATTT+TGG + chr1_1:756313-756332 None:intergenic 5.0%
!!! TATAAAATATTTTAATACAA+TGG - chr1_1:757043-757062 Msa0000100:intron 5.0%
!!! ATAAAATATTTTAATACAAT+GGG - chr1_1:757044-757063 Msa0000100:intron 5.0%
AAGGAACGAGGAATGGGCAT+TGG - chr1_1:755926-755945 Msa0000100:three_prime_UTR 50.0%
! CGCTAAGGAGTACGATGACT+CGG - chr1_1:756028-756047 Msa0000100:three_prime_UTR 50.0%
CGTGAAGCTAAGCTTAAGGG+TGG - chr1_1:756169-756188 Msa0000100:three_prime_UTR 50.0%
CAACCGTGAATATGCTCCAC+AGG - chr1_1:757446-757465 Msa0000100:intron 50.0%
TGACATATGGCTCAACCCTG+TGG + chr1_1:757465-757484 None:intergenic 50.0%
TGAACTGTCCCGACCTAGAT+AGG - chr1_1:757742-757761 Msa0000100:intron 50.0%
GGAGAGATCCTATCTAGGTC+GGG + chr1_1:757753-757772 None:intergenic 50.0%
CTTGACCTATGTCCCGTGTA+TGG - chr1_1:757875-757894 Msa0000100:intron 50.0%
TGTTGGACCTCACTTCAGAG+AGG - chr1_1:758033-758052 Msa0000100:intron 50.0%
AAGCTCAAAGCTCCTTTGGG+TGG - chr1_1:758079-758098 Msa0000100:intron 50.0%
! AAGGTGGTGATGCTGGTAAC+AGG - chr1_1:758131-758150 Msa0000100:intron 50.0%
! AGGTGGTGATGCTGGTAACA+GGG - chr1_1:758132-758151 Msa0000100:intron 50.0%
TGAAGGTGAACGGCGCTTCA+AGG + chr1_1:755813-755832 None:intergenic 55.0%
GCAGCTTCTGCGATTGAGAC+AGG - chr1_1:756515-756534 Msa0000100:intron 55.0%
GACCTATGTCCCGTGTATGG+TGG - chr1_1:757878-757897 Msa0000100:intron 55.0%
CACCACCATACACGGGACAT+AGG + chr1_1:757883-757902 None:intergenic 55.0%
! TGGTGATGCTGGTAACAGGG+AGG - chr1_1:758135-758154 Msa0000100:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 755795 758663 755795 ID=Msa0000100;Name=Msa0000100
chr1_1 mRNA 755795 758663 755795 ID=Msa0000100-mRNA-1;Parent=Msa0000100;Name=Msa0000100-mRNA-1;_AED=0.05;_eAED=0.05;_QI=75|1|1|1|1|1|6|471|244
chr1_1 exon 755795 756481 755795 ID=Msa0000100-mRNA-1:exon:32;Parent=Msa0000100-mRNA-1
chr1_1 exon 756774 756876 756774 ID=Msa0000100-mRNA-1:exon:31;Parent=Msa0000100-mRNA-1
chr1_1 exon 756967 757046 756967 ID=Msa0000100-mRNA-1:exon:30;Parent=Msa0000100-mRNA-1
chr1_1 exon 757922 757979 757922 ID=Msa0000100-mRNA-1:exon:29;Parent=Msa0000100-mRNA-1
chr1_1 exon 758216 758313 758216 ID=Msa0000100-mRNA-1:exon:28;Parent=Msa0000100-mRNA-1
chr1_1 exon 758409 758663 758409 ID=Msa0000100-mRNA-1:exon:27;Parent=Msa0000100-mRNA-1
chr1_1 five_prime_UTR 758589 758663 758589 ID=Msa0000100-mRNA-1:five_prime_utr;Parent=Msa0000100-mRNA-1
chr1_1 CDS 758409 758588 758409 ID=Msa0000100-mRNA-1:cds;Parent=Msa0000100-mRNA-1
chr1_1 CDS 758216 758313 758216 ID=Msa0000100-mRNA-1:cds;Parent=Msa0000100-mRNA-1
chr1_1 CDS 757922 757979 757922 ID=Msa0000100-mRNA-1:cds;Parent=Msa0000100-mRNA-1
chr1_1 CDS 756967 757046 756967 ID=Msa0000100-mRNA-1:cds;Parent=Msa0000100-mRNA-1
chr1_1 CDS 756774 756876 756774 ID=Msa0000100-mRNA-1:cds;Parent=Msa0000100-mRNA-1
chr1_1 CDS 756266 756481 756266 ID=Msa0000100-mRNA-1:cds;Parent=Msa0000100-mRNA-1
chr1_1 three_prime_UTR 755795 756265 755795 ID=Msa0000100-mRNA-1:three_prime_utr;Parent=Msa0000100-mRNA-1
Gene Sequence

>Msa0000100

ATGGCCAAAGAGGAAGTGAAAACCGTTGTTCCTGAATCTGTTTTGAAGAAGCAGAAAAGGAACGAGGAATGGGCATTGGTGAAGAAACAGGATCAAGAATCAGCGAAGAAGAAGAGAGCTGAAACCAGGAAGCTTATCTGGAGTAGAGCCAAACAGTACGCTAAGGAGTACGATGACTCGCAAAAGGAGTTGATTAGGTTGAAGCGTGAAGCTAAGCTTAAGGGTGGATTTTATGTTGATCCTGAAGCTAAGCTTTTGTTCATTATCAGGATCAGAGGTATCAATGCAATGGACCCAAAATCAAGGAAGATTTTGCAGCTTCTGCGATTGAGACAGATCTTCAATGGTGTCTTCCTCAAAGTGAACAAAGCAACCGTGAATATGCTCCACAGGGTTGAGCCATATGTCACCTATGGTTATCCCAATTTGAAGAGTGTCAGAGAATTGATCTACAAGCGTGGTTATGGAAAGGTTGATAGGCAGAGAATTGCTTTGACAGATAACTCTATCATCGAACAGGTTCTTGGGAAACATGGTATCATTTGCATTGAAGATCTCATTCATGAGATCCTTACTGTTGGACCTCACTTCAGAGAGGCAAACAACTTCCTATGGCCTTTCAAGCTCAAAGCTCCTTTGGGTGGCATGAAGAAGAAGAGAAATCACTATGTTGAAGGTGGTGATGCTGGTAACAGGGAGGACTACATCAATGAACTCATTAGAAGGATGAATTAG
Protein sequence

>Msa0000100

MAKEEVKTVVPESVLKKQKRNEEWALVKKQDQESAKKKRAETRKLIWSRAKQYAKEYDDSQKELIRLKREAKLKGGFYVDPEAKLLFIIRIRGINAMDPKSRKILQLLRLRQIFNGVFLKVNKATVNMLHRVEPYVTYGYPNLKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGIICIEDLIHEILTVGPHFREANNFLWPFKLKAPLGGMKKKRNHYVEGGDAGNREDYINELIRRMN*