AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0132560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132560 MtrunA17_Chr1g0201941 91.958 659 26 8 1 654 1 637 0.0 1056
Msa0132560 MtrunA17_Chr6g0463941 54.459 628 194 16 1 587 1 577 2.89e-161 478
Msa0132560 MtrunA17_Chr1g0201911 44.554 606 224 10 2 601 3 502 3.71e-143 428
Msa0132560 MtrunA17_Chr2g0290511 36.170 329 172 6 248 575 76 367 2.79e-52 184
Msa0132560 MtrunA17_Chr2g0290511 43.820 89 47 2 59 145 261 348 1.81e-16 81.6
Msa0132560 MtrunA17_Chr1g0190851 35.077 325 173 8 274 579 224 529 1.03e-43 165
Msa0132560 MtrunA17_Chr1g0190851 41.000 100 56 2 49 145 407 506 1.57e-16 83.2
Msa0132560 MtrunA17_Chr1g0198171 33.546 313 156 10 59 365 113 379 1.17e-39 152
Msa0132560 MtrunA17_Chr1g0198171 40.541 111 62 3 452 559 94 203 5.25e-19 90.9
Msa0132560 MtrunA17_Chr7g0273941 34.050 279 136 6 291 560 241 480 3.36e-39 150
Msa0132560 MtrunA17_Chr7g0273941 44.792 96 43 2 60 145 381 476 3.31e-17 85.1
Msa0132560 MtrunA17_Chr7g0265831 61.165 103 38 2 60 161 115 216 1.18e-32 131
Msa0132560 MtrunA17_Chr7g0265831 43.617 94 51 2 467 559 112 204 5.53e-17 84.3
Msa0132560 MtrunA17_Chr7g0265831 43.750 80 45 0 287 366 285 364 9.06e-14 74.3
Msa0132560 MtrunA17_Chr1g0179191 66.667 87 28 1 60 145 131 217 1.40e-31 129
Msa0132560 MtrunA17_Chr1g0179191 40.602 133 65 5 462 592 122 242 7.79e-20 93.2
Msa0132560 MtrunA17_Chr1g0179191 37.607 117 65 2 259 367 254 370 1.09e-14 77.4
Msa0132560 MtrunA17_Chr5g0409071 33.696 92 56 3 471 561 46 133 9.72e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132560 AT1G76890.2 44.464 569 207 15 57 604 38 518 3.10e-140 421
Msa0132560 AT1G76880.1 53.211 327 102 6 50 369 51 333 4.55e-89 290
Msa0132560 AT5G03680.1 30.952 504 221 13 60 551 119 507 7.03e-57 203
Msa0132560 AT5G03680.1 44.944 89 47 2 470 557 119 206 8.11e-18 87.8
Msa0132560 AT5G28300.1 37.143 280 140 8 291 557 300 556 1.33e-46 174
Msa0132560 AT5G28300.1 40.385 104 51 2 53 145 452 555 5.71e-16 82.0
Msa0132560 AT1G33240.3 66.972 109 31 2 59 165 61 166 7.52e-40 155
Msa0132560 AT1G33240.1 66.972 109 31 2 59 165 61 166 1.69e-39 155
Msa0132560 AT3G10000.1 54.701 117 47 3 35 145 59 175 5.46e-31 127
Msa0132560 AT3G10000.1 43.434 99 55 1 462 559 80 178 7.85e-21 96.7
Msa0132560 AT3G10000.2 51.724 116 42 3 35 145 59 165 1.84e-27 116
Msa0132560 AT3G10000.2 40.816 98 49 1 462 559 80 168 3.28e-18 88.6
Msa0132560 AT5G47660.1 52.632 95 43 1 470 564 303 395 4.59e-23 102
Msa0132560 AT5G47660.1 41.000 100 53 3 60 158 303 397 3.43e-14 75.5
Msa0132560 AT1G33240.2 62.162 74 23 2 94 165 57 127 7.79e-19 91.3
Msa0132560 AT1G33240.2 42.529 87 49 1 60 145 396 482 4.77e-17 85.5
Msa0132560 AT3G25990.1 34.409 93 60 1 471 562 55 147 1.17e-11 67.4

Find 212 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTGTTGCTTCTGTTAT+TGG 0.191531 1_3:+63675752 None:intergenic
TATCTTAACTCTTCTCTAAT+TGG 0.207615 1_3:+63674019 None:intergenic
TTTCGAGATGCAAGTGTTAA+AGG 0.236888 1_3:-63677015 Msa0132560:CDS
GTAGGATTCATATTGATAAT+TGG 0.247032 1_3:+63675638 None:intergenic
GTTGGGCCCAACTTGGTTGC+CGG 0.251390 1_3:+63675020 None:intergenic
TTTAAGCATACGATCGGATA+TGG 0.256367 1_3:-63677046 Msa0132560:CDS
GGTGCAGCTGCTGCTGCTGC+TGG 0.268164 1_3:+63677207 None:intergenic
ACAACAATTGGTGACGAATA+TGG 0.270177 1_3:-63674976 Msa0132560:CDS
AGCAATAGAGACCTTAATTA+AGG 0.274675 1_3:-63673961 Msa0132560:three_prime_UTR
GGTGATGGTGGTTGTGGTTG+TGG 0.281851 1_3:-63674222 Msa0132560:three_prime_UTR
TCCTCCCATAGAGGACCTTT+TGG 0.292057 1_3:+63674822 None:intergenic
TCAGTTGGATGCTTTGTATA+AGG 0.314622 1_3:-63674652 Msa0132560:CDS
ATAAAATACAACCTTAATTA+AGG 0.315567 1_3:+63673950 None:intergenic
ATGTTTATGGTGGTGTAAAT+TGG 0.318061 1_3:-63673913 Msa0132560:three_prime_UTR
GGATTTGCAGAGGAGATTCT+TGG 0.318376 1_3:-63675387 Msa0132560:CDS
GAGAAGGAGCAGCTGCAAAA+AGG 0.318992 1_3:-63674320 Msa0132560:three_prime_UTR
AGCAAAACCACCACAAATTT+CGG 0.324820 1_3:-63675819 Msa0132560:CDS
GCATCCAACTGATGAAAATA+AGG 0.337506 1_3:+63674663 None:intergenic
GATGGTGGTGATGGTGGTTG+TGG 0.340255 1_3:-63674228 Msa0132560:three_prime_UTR
GAAGTAGGGTTTGTTGTTGA+TGG 0.341682 1_3:+63675584 None:intergenic
GGTGGTTGTGGTTGTGGTGG+AGG 0.344192 1_3:-63674216 Msa0132560:three_prime_UTR
AGAAGATGATGAAGAAGATA+TGG 0.346341 1_3:-63674436 Msa0132560:CDS
ATGGACCAAAAGGTCCTCTA+TGG 0.346770 1_3:-63674827 Msa0132560:CDS
GGCACTGAAATTGAAATTGA+TGG 0.352504 1_3:+63675686 None:intergenic
TTAACTTGTACCACTGATAT+TGG 0.352534 1_3:+63675068 None:intergenic
GCAATGGCGGCCACCTCAGC+AGG 0.356286 1_3:-63674532 Msa0132560:CDS
GGACATGTCTTTGAATCTTC+GGG 0.356338 1_3:+63674684 None:intergenic
AGGACATGTCTTTGAATCTT+CGG 0.357417 1_3:+63674683 None:intergenic
TCCTTCTTCCGTTGTGTCTT+CGG 0.361358 1_3:+63675481 None:intergenic
GAAGGATTGGATGGGATTGT+AGG 0.364446 1_3:+63675620 None:intergenic
CCACCTTCCGCCGTCATGTT+AGG 0.366216 1_3:+63677183 None:intergenic
TTGGGCCCAACTTGGTTGCC+GGG 0.367395 1_3:+63675021 None:intergenic
CAACGGTGCCGCCATGCTCT+CGG 0.368345 1_3:+63674572 None:intergenic
ATTGTTGTTGCTGGTGTTGC+GGG 0.369324 1_3:+63674991 None:intergenic
GATGAAAGTGGTGGTGGTGG+TGG 0.369384 1_3:-63674414 Msa0132560:CDS
ACCGCCAGCACAACCACCCC+CGG 0.371351 1_3:-63675039 Msa0132560:CDS
CTCGAAAGAGGAAGCGGAAA+TGG 0.374520 1_3:-63675457 Msa0132560:CDS
GCGGAAGGTGGTGGTGGTGG+TGG 0.380166 1_3:-63677171 Msa0132560:CDS
TCGTCACCAATTGTTGTTGC+TGG 0.391070 1_3:+63674982 None:intergenic
GAGCATGGCGGCACCGTTGA+TGG 0.394589 1_3:-63674568 Msa0132560:CDS
AATAGATCTGTAGAAATGTT+AGG 0.398303 1_3:+63675539 None:intergenic
GGTTGTGGTTGTGGTGGAGG+AGG 0.405531 1_3:-63674213 Msa0132560:three_prime_UTR
TGCTCTCGGGCTGCACCACC+TGG 0.410175 1_3:+63674586 None:intergenic
TGGTGGTGGTGGTAACTTTG+AGG 0.417578 1_3:-63674400 Msa0132560:CDS
TGAAGAACCTCGGTTACAAT+AGG 0.426544 1_3:-63674785 Msa0132560:CDS
GTAGGAGCTAGCCATGGAAT+TGG 0.427286 1_3:+63675514 None:intergenic
ACGGCGGAAGGTGGTGGTGG+TGG 0.432204 1_3:-63677174 Msa0132560:CDS
AGTGCTTGAACTTCAACTTT+CGG 0.432707 1_3:+63674891 None:intergenic
ATCAGGAAGCTAGCAGAACT+TGG 0.434480 1_3:-63676025 Msa0132560:intron
GGAAGAGGGTTGGTGGAAGT+AGG 0.435197 1_3:+63675569 None:intergenic
TGGACAGGAAGAAGAAGATA+TGG 0.441742 1_3:-63674484 Msa0132560:CDS
GAAGAGGGTTGGTGGAAGTA+GGG 0.442303 1_3:+63675570 None:intergenic
TAACAATGGTGAGACTTTCA+TGG 0.445045 1_3:-63674937 Msa0132560:CDS
GATTCATATTGATAATTGGT+TGG 0.446345 1_3:+63675642 None:intergenic
AACGTAGCTTCGGTGGTAAC+CGG 0.450832 1_3:-63677095 Msa0132560:CDS
TAGGGTTTGTTGTTGATGGT+TGG 0.451052 1_3:+63675588 None:intergenic
GTTACAATAGGAATGCAAAA+AGG 0.452050 1_3:-63674773 Msa0132560:CDS
GATGCTTTGTATAAGGAGAA+AGG 0.452352 1_3:-63674645 Msa0132560:CDS
ATTCATATTGATAATTGGTT+GGG 0.453777 1_3:+63675643 None:intergenic
TCAGAGAGTGATGTTGAAAG+AGG 0.453881 1_3:-63677135 Msa0132560:CDS
AACGGTGCCGCCATGCTCTC+GGG 0.453957 1_3:+63674573 None:intergenic
GCAACACCAGCAACAACAAT+TGG 0.455453 1_3:-63674988 Msa0132560:CDS
TGGTGTTGCGGGACTATTGT+TGG 0.455975 1_3:+63675002 None:intergenic
ATTGCGGAACAACAAAATCT+AGG 0.456470 1_3:-63675209 Msa0132560:CDS
AAGTTTCTTGTCGCGGCCAC+CGG 0.458447 1_3:+63677076 None:intergenic
AAAAGATGGAAGAGGGTTGG+TGG 0.469141 1_3:+63675562 None:intergenic
GTAGAAATGTTAGGAAAAGA+TGG 0.472744 1_3:+63675548 None:intergenic
GTTGGTGCTACACTTCTTTG+TGG 0.473011 1_3:+63675110 None:intergenic
GGTGGCCGCCATTGCTGCTC+CGG 0.473929 1_3:+63674540 None:intergenic
GATGGTGGTTGTGGTTGTGG+TGG 0.475121 1_3:-63674219 Msa0132560:three_prime_UTR
ATAAACAAAACTGAGTTTGT+TGG 0.475780 1_3:-63674285 Msa0132560:three_prime_UTR
AAAATAGTTGAAGGATTGGA+TGG 0.481737 1_3:+63675611 None:intergenic
CTTTGTGGGATGAAGTTTCA+AGG 0.482165 1_3:-63676990 Msa0132560:intron
GGTGTTGCGGGACTATTGTT+GGG 0.486909 1_3:+63675003 None:intergenic
ATGACGGCGGAAGGTGGTGG+TGG 0.488218 1_3:-63677177 Msa0132560:CDS
GACTATTGTTGGGCCCAACT+TGG 0.490563 1_3:+63675013 None:intergenic
TGCATTTCTTTGAACTTCTG+TGG 0.490979 1_3:+63675999 None:intergenic
TTGGAAAATAGTTGAAGGAT+TGG 0.493708 1_3:+63675607 None:intergenic
AAATTGGACAAGATTATTTG+AGG 0.496020 1_3:-63673897 Msa0132560:three_prime_UTR
GGTGGTGCAGCCCGAGAGCA+TGG 0.499156 1_3:-63674583 Msa0132560:CDS
TGCAAAGAATCAATAGAGAG+AGG 0.504131 1_3:-63675301 Msa0132560:CDS
TATCTTCTTCTTCCTGTCCA+CGG 0.504848 1_3:+63674487 None:intergenic
GTCGAGGAGGATGGTGGTGA+TGG 0.505301 1_3:-63674237 Msa0132560:three_prime_UTR
GGTTGATGTTGATGAGTTGA+AGG 0.511906 1_3:+63675851 None:intergenic
ATGGATGAAAGTGGTGGTGG+TGG 0.513204 1_3:-63674417 Msa0132560:CDS
CAAGCTGATGCACCCACTGC+AGG 0.513358 1_3:+63674363 None:intergenic
TACCAAGAGAATGGACCAAA+AGG 0.516965 1_3:-63674837 Msa0132560:CDS
GACAACAAGTACCAAGAGAA+TGG 0.517884 1_3:-63674846 Msa0132560:CDS
TGTGTTTGTTGTGGTGGCGG+TGG 0.518161 1_3:+63675155 None:intergenic
AATTGTTGTTGCTGGTGTTG+CGG 0.524953 1_3:+63674990 None:intergenic
TGGGCCCAACTTGGTTGCCG+GGG 0.525453 1_3:+63675022 None:intergenic
TTGTATAAGGAGAAAGGAAA+GGG 0.527542 1_3:-63674639 Msa0132560:CDS
TGGTGGTAACTTTGAGGTAG+TGG 0.527717 1_3:-63674394 Msa0132560:CDS
TCCGAAGACACAACGGAAGA+AGG 0.527908 1_3:-63675482 Msa0132560:CDS
AACAAAACTGAGTTTGTTGG+TGG 0.529537 1_3:-63674282 Msa0132560:three_prime_UTR
CAAAACTGAGTTTGTTGGTG+GGG 0.532718 1_3:-63674280 Msa0132560:three_prime_UTR
TGGTTCAGCCGGAGCAGCAA+TGG 0.533323 1_3:-63674548 Msa0132560:CDS
GAAAATTCTAGTGGTGGAGC+TGG 0.535467 1_3:-63674615 Msa0132560:CDS
GGGCCCAACTTGGTTGCCGG+GGG 0.537475 1_3:+63675023 None:intergenic
ATTGGTGCTACCGCCGGTGT+TGG 0.539807 1_3:+63675086 None:intergenic
TGCTCCGGCTGAACCATCAA+CGG 0.540154 1_3:+63674555 None:intergenic
TGTTGTGTTTGTTGTGGTGG+CGG 0.542486 1_3:+63675152 None:intergenic
GAGGAGGATGGTGGTGATGG+TGG 0.543422 1_3:-63674234 Msa0132560:three_prime_UTR
GAAGATGGTGAACGTAGCTT+CGG 0.543473 1_3:-63677105 Msa0132560:CDS
GGTTGGTATGAAAATGATCA+AGG 0.547374 1_3:+63675873 None:intergenic
ATGTTAGGAAAAGATGGAAG+AGG 0.551505 1_3:+63675554 None:intergenic
GCTACCGCCGGTGTTGGTGT+TGG 0.551728 1_3:+63675092 None:intergenic
GTGGCCGCGACAAGAAACTT+TGG 0.552103 1_3:-63677073 Msa0132560:CDS
AAGGATCTCGAAAGAGGAAG+CGG 0.554153 1_3:-63675463 Msa0132560:CDS
TGGTGGAGCTGGTTCGAACC+AGG 0.556702 1_3:-63674604 Msa0132560:CDS
TGTTGTTGTGTTTGTTGTGG+TGG 0.557187 1_3:+63675149 None:intergenic
AGGAAAAGATGGAAGAGGGT+TGG 0.558092 1_3:+63675559 None:intergenic
TGTTGTTGTTGTGTTTGTTG+TGG 0.560673 1_3:+63675146 None:intergenic
CAAGTGTTAAAGGTCCTTTG+TGG 0.560707 1_3:-63677005 Msa0132560:CDS
GGCACCGTTGATGGTTCAGC+CGG 0.560808 1_3:-63674559 Msa0132560:CDS
CCACCTAACATGACGGCGGA+AGG 0.561648 1_3:-63677186 Msa0132560:CDS
TTCAGCCGGAGCAGCAATGG+CGG 0.565524 1_3:-63674545 Msa0132560:CDS
CGGAAGAAGGATCTCGAAAG+AGG 0.566261 1_3:-63675469 Msa0132560:CDS
TTTGTATAAGGAGAAAGGAA+AGG 0.568622 1_3:-63674640 Msa0132560:CDS
TGGAAGAATTTGAATATGTA+CGG 0.571673 1_3:-63674177 Msa0132560:three_prime_UTR
GATGGTTGGAAAATAGTTGA+AGG 0.571791 1_3:+63675602 None:intergenic
GGTGCTACACTTCTTTGTGG+TGG 0.573648 1_3:+63675113 None:intergenic
GATGGTGAACGTAGCTTCGG+TGG 0.573832 1_3:-63677102 Msa0132560:CDS
GAAAGTGACAGAGAAGCAAG+AGG 0.574790 1_3:-63675408 Msa0132560:CDS
CATGGAGCAGAACCGTGGAC+AGG 0.575210 1_3:-63674499 Msa0132560:CDS
AAAGATATCTCCTCCCATAG+AGG 0.576930 1_3:+63674813 None:intergenic
TTTGAGGAGGGAAATTTGAT+GGG 0.577134 1_3:-63674137 Msa0132560:three_prime_UTR
TTTGCAGAGGAGATTCTTGG+AGG 0.578270 1_3:-63675384 Msa0132560:CDS
AAAATAAGAGAAACTATGCT+AGG 0.578777 1_3:-63677267 Msa0132560:exon
TTGCATTCCTATTGTAACCG+AGG 0.579636 1_3:+63674778 None:intergenic
GAAAGAGGAAGAGTTGAAGA+TGG 0.580801 1_3:-63677120 Msa0132560:CDS
TTGAGGAGGGAAATTTGATG+GGG 0.580811 1_3:-63674136 Msa0132560:three_prime_UTR
AGCGCCAAAGTTTCTTGTCG+CGG 0.581032 1_3:+63677069 None:intergenic
GCAGGAGTTGTCGAGGAGGA+TGG 0.585356 1_3:-63674246 Msa0132560:three_prime_UTR
ATGTTGATGAGTTGAAGGGT+TGG 0.585562 1_3:+63675856 None:intergenic
GGTGGGGTCATAGATAGTGC+AGG 0.587167 1_3:-63674264 Msa0132560:three_prime_UTR
AACAATGGTGAGACTTTCAT+GGG 0.588661 1_3:-63674936 Msa0132560:CDS
GGTCATTGAGAAACGCGAGC+AGG 0.589179 1_3:-63675363 Msa0132560:CDS
AAAGAGGAAGCGGAAATGGA+AGG 0.590185 1_3:-63675453 Msa0132560:CDS
AGATCTATTATCCAATTCCA+TGG 0.590202 1_3:-63675525 Msa0132560:CDS
GAAGAAGATATGGATGAAAG+TGG 0.590417 1_3:-63674426 Msa0132560:CDS
AAGTGTTAAAGGTCCTTTGT+GGG 0.591969 1_3:-63677004 Msa0132560:CDS
ACTGATATTGGTGCTACCGC+CGG 0.592470 1_3:+63675080 None:intergenic
TATCACAAGAGAACCAAAGA+TGG 0.592914 1_3:-63675950 Msa0132560:CDS
AAATAGTTGAAGGATTGGAT+GGG 0.593793 1_3:+63675612 None:intergenic
TGGACCAAAAGGTCCTCTAT+GGG 0.594236 1_3:-63674826 Msa0132560:CDS
TAGTATAGTAGCTAGTGAGA+AGG 0.594977 1_3:-63674336 Msa0132560:three_prime_UTR
GCAAAGAATCAATAGAGAGA+GGG 0.595028 1_3:-63675300 Msa0132560:CDS
TGTTAGGAAAAGATGGAAGA+GGG 0.595844 1_3:+63675555 None:intergenic
ATGTTATCTTCTCCCTGCTG+AGG 0.596975 1_3:+63674519 None:intergenic
ATGGTGAGACTTTCATGGGA+GGG 0.597885 1_3:-63674932 Msa0132560:CDS
CCTTCCGCCGTCATGTTAGG+TGG 0.598899 1_3:+63677186 None:intergenic
ATGTACGGTGGAATGACACA+TGG 0.599343 1_3:-63674162 Msa0132560:three_prime_UTR
GAGGAGGAGAGATTCTCTTG+TGG 0.600742 1_3:-63674197 Msa0132560:three_prime_UTR
ACAAAACTGAGTTTGTTGGT+GGG 0.601717 1_3:-63674281 Msa0132560:three_prime_UTR
GGTGTTGGTGCCGAAATTTG+TGG 0.601888 1_3:+63675809 None:intergenic
TGTAGCACCAACACCAACAC+CGG 0.603439 1_3:-63675099 Msa0132560:CDS
AGATAGTGCAGGAGTTGTCG+AGG 0.606737 1_3:-63674253 Msa0132560:three_prime_UTR
AGAAGCAACAATGTCACAAG+TGG 0.606912 1_3:-63675744 Msa0132560:CDS
GGCCGAGGTGGAAAATCAGA+TGG 0.609437 1_3:-63675929 Msa0132560:CDS
AGTGGCAAACAAACCTGCAG+TGG 0.609531 1_3:-63674376 Msa0132560:CDS
AGAACCAAAGATGGCCGAGG+TGG 0.611661 1_3:-63675941 Msa0132560:CDS
AACATGACGGCGGAAGGTGG+TGG 0.613643 1_3:-63677180 Msa0132560:CDS
ATGGATGAAGATGATAAAGA+TGG 0.614880 1_3:-63674465 Msa0132560:CDS
AAGAGAACCAAAGATGGCCG+AGG 0.615515 1_3:-63675944 Msa0132560:CDS
GATAAATCTGAGGACGAGCA+TGG 0.616417 1_3:-63674868 Msa0132560:CDS
AGCTGCACCACCTAACATGA+CGG 0.617434 1_3:-63677193 Msa0132560:CDS
AAGAATTTGAATATGTACGG+TGG 0.618964 1_3:-63674174 Msa0132560:three_prime_UTR
TGAGGAGGGAAATTTGATGG+GGG 0.620174 1_3:-63674135 Msa0132560:three_prime_UTR
CCTAACATGACGGCGGAAGG+TGG 0.623080 1_3:-63677183 Msa0132560:CDS
TCAGGAAGCTAGCAGAACTT+GGG 0.624015 1_3:-63676024 Msa0132560:intron
CAATGGCGGCCACCTCAGCA+GGG 0.624870 1_3:-63674531 Msa0132560:CDS
GTTGATGTTGATGAGTTGAA+GGG 0.625077 1_3:+63675852 None:intergenic
GTTGGTATGAAAATGATCAA+GGG 0.626104 1_3:+63675874 None:intergenic
TTATCTTCTCCCTGCTGAGG+TGG 0.627005 1_3:+63674522 None:intergenic
GATATGGATGAAAGTGGTGG+TGG 0.629728 1_3:-63674420 Msa0132560:CDS
AAGGGAGAAAATTCTAGTGG+TGG 0.630570 1_3:-63674621 Msa0132560:CDS
GTCTTCGGAAGACGTCGAGT+AGG 0.632078 1_3:+63675496 None:intergenic
TCTTTAGCAATGAAGAACCT+CGG 0.632836 1_3:-63674795 Msa0132560:CDS
AGAAGCAAGAGGATTTGCAG+AGG 0.635450 1_3:-63675397 Msa0132560:CDS
AGAGTTAAGATAGAGAGAGT+CGG 0.643314 1_3:-63674008 Msa0132560:three_prime_UTR
GTAGCTTCGGTGGTAACCGG+TGG 0.643526 1_3:-63677092 Msa0132560:CDS
TTCAAGCACTGATAAATCTG+AGG 0.643720 1_3:-63674878 Msa0132560:CDS
TAGTGCAGGAGTTGTCGAGG+AGG 0.646241 1_3:-63674250 Msa0132560:three_prime_UTR
GACGTCTTCCGAAGACACAA+CGG 0.647187 1_3:-63675489 Msa0132560:CDS
TGAAGATGATAAAGATGGTG+AGG 0.649200 1_3:-63674460 Msa0132560:CDS
GTAACAACTTGTGACGGTGT+TGG 0.650468 1_3:+63675794 None:intergenic
GTTGGTGCCGAAATTTGTGG+TGG 0.654114 1_3:+63675812 None:intergenic
TGCATGAGGGATACTTAGAG+TGG 0.657060 1_3:+63675715 None:intergenic
GGAAAGGGAGAAAATTCTAG+TGG 0.661145 1_3:-63674624 Msa0132560:CDS
GTGGCAAACAAACCTGCAGT+GGG 0.663059 1_3:-63674375 Msa0132560:CDS
CGTCGAGTAGGAGCTAGCCA+TGG 0.663851 1_3:+63675508 None:intergenic
ACCAAAAGGTCCTCTATGGG+AGG 0.664112 1_3:-63674823 Msa0132560:CDS
TCAGCAGGGAGAAGATAACA+TGG 0.666074 1_3:-63674517 Msa0132560:CDS
TTGATGGAACAGTTGCATGA+GGG 0.669086 1_3:+63675702 None:intergenic
GAGTTGTTAGAGAAGAAGCG+TGG 0.669199 1_3:-63675337 Msa0132560:CDS
TGGGAGGGATGAGTTCGTCA+AGG 0.669391 1_3:-63674917 Msa0132560:CDS
ATTGATGGAACAGTTGCATG+AGG 0.676758 1_3:+63675701 None:intergenic
GGAGTTGTCGAGGAGGATGG+TGG 0.678808 1_3:-63674243 Msa0132560:three_prime_UTR
GCAGTTGTAACAACTTGTGA+CGG 0.679663 1_3:+63675788 None:intergenic
GATAACATGGAGCAGAACCG+TGG 0.681082 1_3:-63674504 Msa0132560:CDS
ATTGTGAAAGTTGATAACAA+TGG 0.690514 1_3:-63674951 Msa0132560:CDS
AATGGTGAGACTTTCATGGG+AGG 0.694246 1_3:-63674933 Msa0132560:CDS
TGTACGGTGGAATGACACAT+GGG 0.698113 1_3:-63674161 Msa0132560:three_prime_UTR
CTTGAAACTTCATCCCACAA+AGG 0.698850 1_3:+63676991 None:intergenic
GGCGGTAGCACCAATATCAG+TGG 0.699903 1_3:-63675078 Msa0132560:CDS
GAGGGATGAGTTCGTCAAGG+TGG 0.700639 1_3:-63674914 Msa0132560:CDS
TGGAGCTGGTTCGAACCAGG+TGG 0.701524 1_3:-63674601 Msa0132560:CDS
TGCACCACCTAACATGACGG+CGG 0.703359 1_3:-63677190 Msa0132560:CDS
AGCACCAACACCAACACCGG+CGG 0.703596 1_3:-63675096 Msa0132560:CDS
GGTGCAGCCCGAGAGCATGG+CGG 0.703630 1_3:-63674580 Msa0132560:CDS
GAAGATATGGATGAAAGTGG+TGG 0.720036 1_3:-63674423 Msa0132560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACAAAAATTTAGTTTATT+TGG - chr1_3:63674422-63674441 Msa0132560:CDS 10.0%
!! ATGAAAAAATAAATTAGAAA+AGG + chr1_3:63674653-63674672 None:intergenic 10.0%
!!! TAAAATTTGTTTATTTTTTG+GGG - chr1_3:63677283-63677302 Msa0132560:five_prime_UTR 10.0%
!! AAAAAATAAATTAGAAAAGG+AGG + chr1_3:63674650-63674669 None:intergenic 15.0%
!!! ATTTATTTTTTCATTGCTTT+TGG - chr1_3:63674659-63674678 Msa0132560:CDS 15.0%
!! TAAACAAGAAATAAAAACAT+GGG + chr1_3:63675147-63675166 None:intergenic 15.0%
!! ATAAACAAGAAATAAAAACA+TGG + chr1_3:63675148-63675167 None:intergenic 15.0%
!!! AAAATTTGTTTATTTTTTGG+GGG - chr1_3:63677284-63677303 Msa0132560:five_prime_UTR 15.0%
!! ATAAAATACAACCTTAATTA+AGG + chr1_3:63677400-63677419 None:intergenic 15.0%
!!! TGAATTCTTTGAATTTTGAT+GGG + chr1_3:63673981-63674000 None:intergenic 20.0%
!! AAAGTAACTGAAATAGATAA+AGG + chr1_3:63675045-63675064 None:intergenic 20.0%
!! ACAAATGCATGATATAAAAA+AGG + chr1_3:63675200-63675219 None:intergenic 20.0%
!! TCTCACTTTAATTATTAATC+AGG - chr1_3:63675305-63675324 Msa0132560:CDS 20.0%
!!! ATTCATATTGATAATTGGTT+GGG + chr1_3:63675707-63675726 None:intergenic 20.0%
!!! AAATTTGTTTATTTTTTGGG+GGG - chr1_3:63677285-63677304 Msa0132560:five_prime_UTR 20.0%
!!! TTCATCTTGTTTTATGTTTA+TGG - chr1_3:63677421-63677440 Msa0132560:five_prime_UTR 20.0%
!!! GAATTCTTTGAATTTTGATG+GGG + chr1_3:63673980-63673999 None:intergenic 25.0%
!! GTGAATTCTTTGAATTTTGA+TGG + chr1_3:63673982-63674001 None:intergenic 25.0%
! AACTGAGAGAAAAAAAATAG+AGG + chr1_3:63674007-63674026 None:intergenic 25.0%
! AAAATAAGAGAAACTATGCT+AGG - chr1_3:63674080-63674099 Msa0132560:three_prime_UTR 25.0%
! AATACAAAAGACTAAGAAAC+CGG + chr1_3:63674608-63674627 None:intergenic 25.0%
!! ATTAGAAAAGGAGGATTTTA+TGG + chr1_3:63674641-63674660 None:intergenic 25.0%
!!! TATTTTTTCATTGCTTTTGG+TGG - chr1_3:63674662-63674681 Msa0132560:CDS 25.0%
! ATGTGAAGTCTATATGATTT+GGG - chr1_3:63674730-63674749 Msa0132560:CDS 25.0%
!!! ATTCAAGTTGGTAACTTATT+TGG + chr1_3:63674813-63674832 None:intergenic 25.0%
!! TTTGATAACTCAATTCAAGT+TGG + chr1_3:63674825-63674844 None:intergenic 25.0%
!! CATTTCTTTTCATCAATTCA+TGG - chr1_3:63674956-63674975 Msa0132560:CDS 25.0%
!! GATTCATATTGATAATTGGT+TGG + chr1_3:63675708-63675727 None:intergenic 25.0%
!! GTAGGATTCATATTGATAAT+TGG + chr1_3:63675712-63675731 None:intergenic 25.0%
! AATAGATCTGTAGAAATGTT+AGG + chr1_3:63675811-63675830 None:intergenic 25.0%
!!! ATTGTGAAAGTTGATAACAA+TGG - chr1_3:63676396-63676415 Msa0132560:intron 25.0%
!! AAAAAGGTGCAAAGAAAAAT+GGG - chr1_3:63676590-63676609 Msa0132560:intron 25.0%
! ATAAACAAAACTGAGTTTGT+TGG - chr1_3:63677062-63677081 Msa0132560:CDS 25.0%
! TGGAAGAATTTGAATATGTA+CGG - chr1_3:63677170-63677189 Msa0132560:CDS 25.0%
! TATCTTAACTCTTCTCTAAT+TGG + chr1_3:63677331-63677350 None:intergenic 25.0%
!!! ATCTTGTTTTATGTTTATGG+TGG - chr1_3:63677424-63677443 Msa0132560:five_prime_UTR 25.0%
! AAATTGGACAAGATTATTTG+AGG - chr1_3:63677450-63677469 Msa0132560:five_prime_UTR 25.0%
TGAGAGAAAAAAAATAGAGG+TGG + chr1_3:63674004-63674023 None:intergenic 30.0%
AAGAAGCAAAAACCAAATGA+TGG + chr1_3:63674582-63674601 None:intergenic 30.0%
!! TTGGTTTTTGCTTCTTTTTC+CGG - chr1_3:63674586-63674605 Msa0132560:CDS 30.0%
GATGTGAAGTCTATATGATT+TGG - chr1_3:63674729-63674748 Msa0132560:CDS 30.0%
!! AGTTGGTAACCGAAATAAAA+GGG + chr1_3:63674866-63674885 None:intergenic 30.0%
AAAATGCATGAACTGAAGTT+AGG + chr1_3:63674926-63674945 None:intergenic 30.0%
! TTCAGTTCATGCATTTTTCA+TGG - chr1_3:63674929-63674948 Msa0132560:CDS 30.0%
! TCAGTTCATGCATTTTTCAT+GGG - chr1_3:63674930-63674949 Msa0132560:CDS 30.0%
AAGTAGTGTTAATTGCAACA+TGG + chr1_3:63675083-63675102 None:intergenic 30.0%
GTTGGTATGAAAATGATCAA+GGG + chr1_3:63675476-63675495 None:intergenic 30.0%
!! GTAGTTGCAATATTGTTTTG+AGG + chr1_3:63675685-63675704 None:intergenic 30.0%
AAATAGTTGAAGGATTGGAT+GGG + chr1_3:63675738-63675757 None:intergenic 30.0%
AAAATAGTTGAAGGATTGGA+TGG + chr1_3:63675739-63675758 None:intergenic 30.0%
TTGGAAAATAGTTGAAGGAT+TGG + chr1_3:63675743-63675762 None:intergenic 30.0%
GTAGAAATGTTAGGAAAAGA+TGG + chr1_3:63675802-63675821 None:intergenic 30.0%
AGATCTATTATCCAATTCCA+TGG - chr1_3:63675822-63675841 Msa0132560:CDS 30.0%
GTCTTTCTTGCAAAAAATTG+CGG - chr1_3:63676122-63676141 Msa0132560:intron 30.0%
!! TTAACTTGTACCACTGATAT+TGG + chr1_3:63676282-63676301 None:intergenic 30.0%
GTTACAATAGGAATGCAAAA+AGG - chr1_3:63676574-63676593 Msa0132560:intron 30.0%
! CAAAAAGGTGCAAAGAAAAA+TGG - chr1_3:63676589-63676608 Msa0132560:intron 30.0%
!!! ATGTCCTTATTTTCATCAGT+TGG - chr1_3:63676680-63676699 Msa0132560:intron 30.0%
TTTGTATAAGGAGAAAGGAA+AGG - chr1_3:63676707-63676726 Msa0132560:intron 30.0%
TTGTATAAGGAGAAAGGAAA+GGG - chr1_3:63676708-63676727 Msa0132560:intron 30.0%
ATGGATGAAGATGATAAAGA+TGG - chr1_3:63676882-63676901 Msa0132560:intron 30.0%
AGAAGATGATGAAGAAGATA+TGG - chr1_3:63676911-63676930 Msa0132560:intron 30.0%
AAGAATTTGAATATGTACGG+TGG - chr1_3:63677173-63677192 Msa0132560:CDS 30.0%
AGCAATAGAGACCTTAATTA+AGG - chr1_3:63677386-63677405 Msa0132560:five_prime_UTR 30.0%
! ATGTTTATGGTGGTGTAAAT+TGG - chr1_3:63677434-63677453 Msa0132560:five_prime_UTR 30.0%
TTTAAGCATACGATCGGATA+TGG - chr1_3:63674301-63674320 Msa0132560:three_prime_UTR 35.0%
TTTCGAGATGCAAGTGTTAA+AGG - chr1_3:63674332-63674351 Msa0132560:three_prime_UTR 35.0%
AAGTGTTAAAGGTCCTTTGT+GGG - chr1_3:63674343-63674362 Msa0132560:exon 35.0%
ATTCATGTGATGTAGAGATC+TGG - chr1_3:63674535-63674554 Msa0132560:CDS 35.0%
TTCATGTGATGTAGAGATCT+GGG - chr1_3:63674536-63674555 Msa0132560:CDS 35.0%
!! TTCTTTCCCCCCTTTTATTT+CGG - chr1_3:63674854-63674873 Msa0132560:CDS 35.0%
! TTGGTAACCGAAATAAAAGG+GGG + chr1_3:63674864-63674883 None:intergenic 35.0%
!! GTTGGTAACCGAAATAAAAG+GGG + chr1_3:63674865-63674884 None:intergenic 35.0%
!! GAGTTGGTAACCGAAATAAA+AGG + chr1_3:63674867-63674886 None:intergenic 35.0%
AATTGCAACATGGAAATGCA+AGG + chr1_3:63675073-63675092 None:intergenic 35.0%
TGCATTTCTTTGAACTTCTG+TGG + chr1_3:63675351-63675370 None:intergenic 35.0%
TATCACAAGAGAACCAAAGA+TGG - chr1_3:63675397-63675416 Msa0132560:CDS 35.0%
! GGTTGGTATGAAAATGATCA+AGG + chr1_3:63675477-63675496 None:intergenic 35.0%
! GTTGATGTTGATGAGTTGAA+GGG + chr1_3:63675498-63675517 None:intergenic 35.0%
AGCAAAACCACCACAAATTT+CGG - chr1_3:63675528-63675547 Msa0132560:CDS 35.0%
! GACATTGTTGCTTCTGTTAT+TGG + chr1_3:63675598-63675617 None:intergenic 35.0%
! GGCACTGAAATTGAAATTGA+TGG + chr1_3:63675664-63675683 None:intergenic 35.0%
GATGGTTGGAAAATAGTTGA+AGG + chr1_3:63675748-63675767 None:intergenic 35.0%
TGTTAGGAAAAGATGGAAGA+GGG + chr1_3:63675795-63675814 None:intergenic 35.0%
ATGTTAGGAAAAGATGGAAG+AGG + chr1_3:63675796-63675815 None:intergenic 35.0%
TGCAAAGAATCAATAGAGAG+AGG - chr1_3:63676046-63676065 Msa0132560:intron 35.0%
GCAAAGAATCAATAGAGAGA+GGG - chr1_3:63676047-63676066 Msa0132560:intron 35.0%
ATTGCGGAACAACAAAATCT+AGG - chr1_3:63676138-63676157 Msa0132560:intron 35.0%
! TGTTGTTGTTGTGTTTGTTG+TGG + chr1_3:63676204-63676223 None:intergenic 35.0%
!! ACAACAATTGGTGACGAATA+TGG - chr1_3:63676371-63676390 Msa0132560:intron 35.0%
TAACAATGGTGAGACTTTCA+TGG - chr1_3:63676410-63676429 Msa0132560:intron 35.0%
AACAATGGTGAGACTTTCAT+GGG - chr1_3:63676411-63676430 Msa0132560:intron 35.0%
AGTGCTTGAACTTCAACTTT+CGG + chr1_3:63676459-63676478 None:intergenic 35.0%
!! TTCAAGCACTGATAAATCTG+AGG - chr1_3:63676469-63676488 Msa0132560:intron 35.0%
TCTTTAGCAATGAAGAACCT+CGG - chr1_3:63676552-63676571 Msa0132560:intron 35.0%
AGGACATGTCTTTGAATCTT+CGG + chr1_3:63676667-63676686 None:intergenic 35.0%
GCATCCAACTGATGAAAATA+AGG + chr1_3:63676687-63676706 None:intergenic 35.0%
TCAGTTGGATGCTTTGTATA+AGG - chr1_3:63676695-63676714 Msa0132560:intron 35.0%
GATGCTTTGTATAAGGAGAA+AGG - chr1_3:63676702-63676721 Msa0132560:intron 35.0%
TGAAGATGATAAAGATGGTG+AGG - chr1_3:63676887-63676906 Msa0132560:intron 35.0%
GAAGAAGATATGGATGAAAG+TGG - chr1_3:63676921-63676940 Msa0132560:intron 35.0%
TAGTATAGTAGCTAGTGAGA+AGG - chr1_3:63677011-63677030 Msa0132560:CDS 35.0%
!! AACAAAACTGAGTTTGTTGG+TGG - chr1_3:63677065-63677084 Msa0132560:CDS 35.0%
!! ACAAAACTGAGTTTGTTGGT+GGG - chr1_3:63677066-63677085 Msa0132560:CDS 35.0%
!! TTTTGAGGAGGGAAATTTGA+TGG - chr1_3:63677209-63677228 Msa0132560:CDS 35.0%
TTTGAGGAGGGAAATTTGAT+GGG - chr1_3:63677210-63677229 Msa0132560:CDS 35.0%
AGAGTTAAGATAGAGAGAGT+CGG - chr1_3:63677339-63677358 Msa0132560:five_prime_UTR 35.0%
TCAGAGAGTGATGTTGAAAG+AGG - chr1_3:63674212-63674231 Msa0132560:three_prime_UTR 40.0%
GAAAGAGGAAGAGTTGAAGA+TGG - chr1_3:63674227-63674246 Msa0132560:three_prime_UTR 40.0%
CAAGTGTTAAAGGTCCTTTG+TGG - chr1_3:63674342-63674361 Msa0132560:three_prime_UTR 40.0%
CTTGAAACTTCATCCCACAA+AGG + chr1_3:63674359-63674378 None:intergenic 40.0%
CTTTGTGGGATGAAGTTTCA+AGG - chr1_3:63674357-63674376 Msa0132560:exon 40.0%
CTTGCTTCAACTCCATCATT+TGG - chr1_3:63674567-63674586 Msa0132560:CDS 40.0%
TGGTAACCGAAATAAAAGGG+GGG + chr1_3:63674863-63674882 None:intergenic 40.0%
ATTTCGGTTACCAACTCTCA+TGG - chr1_3:63674870-63674889 Msa0132560:CDS 40.0%
TTTCGGTTACCAACTCTCAT+GGG - chr1_3:63674871-63674890 Msa0132560:CDS 40.0%
CTTCAATTTCCCATGAGAGT+TGG + chr1_3:63674883-63674902 None:intergenic 40.0%
! TACCATCTGATTTTCCACCT+CGG + chr1_3:63675423-63675442 None:intergenic 40.0%
! ATGTTGATGAGTTGAAGGGT+TGG + chr1_3:63675494-63675513 None:intergenic 40.0%
! GGTTGATGTTGATGAGTTGA+AGG + chr1_3:63675499-63675518 None:intergenic 40.0%
!! GGTGGTTTTGCTATGTTTTG+AGG + chr1_3:63675520-63675539 None:intergenic 40.0%
GCAGTTGTAACAACTTGTGA+CGG + chr1_3:63675562-63675581 None:intergenic 40.0%
AGAAGCAACAATGTCACAAG+TGG - chr1_3:63675603-63675622 Msa0132560:CDS 40.0%
TTGATGGAACAGTTGCATGA+GGG + chr1_3:63675648-63675667 None:intergenic 40.0%
ATTGATGGAACAGTTGCATG+AGG + chr1_3:63675649-63675668 None:intergenic 40.0%
!! TAGGGTTTGTTGTTGATGGT+TGG + chr1_3:63675762-63675781 None:intergenic 40.0%
!! GAAGTAGGGTTTGTTGTTGA+TGG + chr1_3:63675766-63675785 None:intergenic 40.0%
!! TGTTGTTGTGTTTGTTGTGG+TGG + chr1_3:63676201-63676220 None:intergenic 40.0%
! AATTGTTGTTGCTGGTGTTG+CGG + chr1_3:63676360-63676379 None:intergenic 40.0%
GACAACAAGTACCAAGAGAA+TGG - chr1_3:63676501-63676520 Msa0132560:intron 40.0%
TACCAAGAGAATGGACCAAA+AGG - chr1_3:63676510-63676529 Msa0132560:intron 40.0%
AAAGATATCTCCTCCCATAG+AGG + chr1_3:63676537-63676556 None:intergenic 40.0%
TGAAGAACCTCGGTTACAAT+AGG - chr1_3:63676562-63676581 Msa0132560:intron 40.0%
TTGCATTCCTATTGTAACCG+AGG + chr1_3:63676572-63676591 None:intergenic 40.0%
GGACATGTCTTTGAATCTTC+GGG + chr1_3:63676666-63676685 None:intergenic 40.0%
GGAAAGGGAGAAAATTCTAG+TGG - chr1_3:63676723-63676742 Msa0132560:intron 40.0%
AAGGGAGAAAATTCTAGTGG+TGG - chr1_3:63676726-63676745 Msa0132560:intron 40.0%
TATCTTCTTCTTCCTGTCCA+CGG + chr1_3:63676863-63676882 None:intergenic 40.0%
TGGACAGGAAGAAGAAGATA+TGG - chr1_3:63676863-63676882 Msa0132560:intron 40.0%
GAAGATATGGATGAAAGTGG+TGG - chr1_3:63676924-63676943 Msa0132560:intron 40.0%
!! CAAAACTGAGTTTGTTGGTG+GGG - chr1_3:63677067-63677086 Msa0132560:CDS 40.0%
TTGAGGAGGGAAATTTGATG+GGG - chr1_3:63677211-63677230 Msa0132560:CDS 40.0%
!! GGTGACTCAGCTGTTTTAAG+TGG - chr1_3:63674101-63674120 Msa0132560:three_prime_UTR 45.0%
!! GACTCAGCTGTTTTAAGTGG+TGG - chr1_3:63674104-63674123 Msa0132560:three_prime_UTR 45.0%
!! TCAGCTGTTTTAAGTGGTGG+CGG - chr1_3:63674107-63674126 Msa0132560:three_prime_UTR 45.0%
GAAGATGGTGAACGTAGCTT+CGG - chr1_3:63674242-63674261 Msa0132560:three_prime_UTR 45.0%
! GGCGCTTTTAAGCATACGAT+CGG - chr1_3:63674295-63674314 Msa0132560:three_prime_UTR 45.0%
ATCAGGAAGCTAGCAGAACT+TGG - chr1_3:63675322-63675341 Msa0132560:CDS 45.0%
TCAGGAAGCTAGCAGAACTT+GGG - chr1_3:63675323-63675342 Msa0132560:CDS 45.0%
GTAACAACTTGTGACGGTGT+TGG + chr1_3:63675556-63675575 None:intergenic 45.0%
TGCATGAGGGATACTTAGAG+TGG + chr1_3:63675635-63675654 None:intergenic 45.0%
GAAGGATTGGATGGGATTGT+AGG + chr1_3:63675730-63675749 None:intergenic 45.0%
AAAAGATGGAAGAGGGTTGG+TGG + chr1_3:63675788-63675807 None:intergenic 45.0%
AGGAAAAGATGGAAGAGGGT+TGG + chr1_3:63675791-63675810 None:intergenic 45.0%
TCCTTCTTCCGTTGTGTCTT+CGG + chr1_3:63675869-63675888 None:intergenic 45.0%
AAGGATCTCGAAAGAGGAAG+CGG - chr1_3:63675884-63675903 Msa0132560:CDS 45.0%
AAAGAGGAAGCGGAAATGGA+AGG - chr1_3:63675894-63675913 Msa0132560:CDS 45.0%
GAAAGTGACAGAGAAGCAAG+AGG - chr1_3:63675939-63675958 Msa0132560:CDS 45.0%
AGAAGCAAGAGGATTTGCAG+AGG - chr1_3:63675950-63675969 Msa0132560:CDS 45.0%
GGATTTGCAGAGGAGATTCT+TGG - chr1_3:63675960-63675979 Msa0132560:CDS 45.0%
TTTGCAGAGGAGATTCTTGG+AGG - chr1_3:63675963-63675982 Msa0132560:CDS 45.0%
GAGTTGTTAGAGAAGAAGCG+TGG - chr1_3:63676010-63676029 Msa0132560:CDS 45.0%
!! TGTTGTGTTTGTTGTGGTGG+CGG + chr1_3:63676198-63676217 None:intergenic 45.0%
!! GTTGGTGCTACACTTCTTTG+TGG + chr1_3:63676240-63676259 None:intergenic 45.0%
!! ATTGTTGTTGCTGGTGTTGC+GGG + chr1_3:63676359-63676378 None:intergenic 45.0%
GCAACACCAGCAACAACAAT+TGG - chr1_3:63676359-63676378 Msa0132560:intron 45.0%
! TCGTCACCAATTGTTGTTGC+TGG + chr1_3:63676368-63676387 None:intergenic 45.0%
AATGGTGAGACTTTCATGGG+AGG - chr1_3:63676414-63676433 Msa0132560:intron 45.0%
ATGGTGAGACTTTCATGGGA+GGG - chr1_3:63676415-63676434 Msa0132560:intron 45.0%
GATAAATCTGAGGACGAGCA+TGG - chr1_3:63676479-63676498 Msa0132560:intron 45.0%
! GACCTTTTGGTCCATTCTCT+TGG + chr1_3:63676515-63676534 None:intergenic 45.0%
ATGGACCAAAAGGTCCTCTA+TGG - chr1_3:63676520-63676539 Msa0132560:intron 45.0%
TGGACCAAAAGGTCCTCTAT+GGG - chr1_3:63676521-63676540 Msa0132560:intron 45.0%
! GAAAATTCTAGTGGTGGAGC+TGG - chr1_3:63676732-63676751 Msa0132560:intron 45.0%
ATGTTATCTTCTCCCTGCTG+AGG + chr1_3:63676831-63676850 None:intergenic 45.0%
TCAGCAGGGAGAAGATAACA+TGG - chr1_3:63676830-63676849 Msa0132560:intron 45.0%
GATATGGATGAAAGTGGTGG+TGG - chr1_3:63676927-63676946 Msa0132560:intron 45.0%
!! TGGTGGTAACTTTGAGGTAG+TGG - chr1_3:63676953-63676972 Msa0132560:intron 45.0%
AGAAGGAGCAGCTGCAAAAA+GGG - chr1_3:63677028-63677047 Msa0132560:CDS 45.0%
ATGTACGGTGGAATGACACA+TGG - chr1_3:63677185-63677204 Msa0132560:CDS 45.0%
TGTACGGTGGAATGACACAT+GGG - chr1_3:63677186-63677205 Msa0132560:CDS 45.0%
! GGAATGACACATGGGTTTTG+AGG - chr1_3:63677194-63677213 Msa0132560:CDS 45.0%
!!! ATGACACATGGGTTTTGAGG+AGG - chr1_3:63677197-63677216 Msa0132560:CDS 45.0%
!!! TGACACATGGGTTTTGAGGA+GGG - chr1_3:63677198-63677217 Msa0132560:CDS 45.0%
TGAGGAGGGAAATTTGATGG+GGG - chr1_3:63677212-63677231 Msa0132560:CDS 45.0%
!!! TTTACTTTTTTTTTTTTTTT+TGG - chr1_3:63675273-63675292 Msa0132560:CDS 5.0%
!!! TATAAAATTTGTTTATTTTT+TGG - chr1_3:63677281-63677300 Msa0132560:five_prime_UTR 5.0%
!!! ATAAAATTTGTTTATTTTTT+GGG - chr1_3:63677282-63677301 Msa0132560:five_prime_UTR 5.0%
AGCTGCACCACCTAACATGA+CGG - chr1_3:63674154-63674173 Msa0132560:three_prime_UTR 50.0%
AACGTAGCTTCGGTGGTAAC+CGG - chr1_3:63674252-63674271 Msa0132560:three_prime_UTR 50.0%
AGCGCCAAAGTTTCTTGTCG+CGG + chr1_3:63674281-63674300 None:intergenic 50.0%
AAGAGAACCAAAGATGGCCG+AGG - chr1_3:63675403-63675422 Msa0132560:CDS 50.0%
! TTTTCCACCTCGGCCATCTT+TGG + chr1_3:63675413-63675432 None:intergenic 50.0%
!! GTTGGTGCCGAAATTTGTGG+TGG + chr1_3:63675538-63675557 None:intergenic 50.0%
!! GGTGTTGGTGCCGAAATTTG+TGG + chr1_3:63675541-63675560 None:intergenic 50.0%
!! GAAGAGGGTTGGTGGAAGTA+GGG + chr1_3:63675780-63675799 None:intergenic 50.0%
GTAGGAGCTAGCCATGGAAT+TGG + chr1_3:63675836-63675855 None:intergenic 50.0%
GACGTCTTCCGAAGACACAA+CGG - chr1_3:63675858-63675877 Msa0132560:CDS 50.0%
TCCGAAGACACAACGGAAGA+AGG - chr1_3:63675865-63675884 Msa0132560:CDS 50.0%
CGGAAGAAGGATCTCGAAAG+AGG - chr1_3:63675878-63675897 Msa0132560:CDS 50.0%
CTCGAAAGAGGAAGCGGAAA+TGG - chr1_3:63675890-63675909 Msa0132560:CDS 50.0%
! GGTGCTACACTTCTTTGTGG+TGG + chr1_3:63676237-63676256 None:intergenic 50.0%
TGTAGCACCAACACCAACAC+CGG - chr1_3:63676248-63676267 Msa0132560:intron 50.0%
!! ACTGATATTGGTGCTACCGC+CGG + chr1_3:63676270-63676289 None:intergenic 50.0%
GACTATTGTTGGGCCCAACT+TGG + chr1_3:63676337-63676356 None:intergenic 50.0%
! GGTGTTGCGGGACTATTGTT+GGG + chr1_3:63676347-63676366 None:intergenic 50.0%
!! TGGTGTTGCGGGACTATTGT+TGG + chr1_3:63676348-63676367 None:intergenic 50.0%
ACCAAAAGGTCCTCTATGGG+AGG - chr1_3:63676524-63676543 Msa0132560:intron 50.0%
! TCCTCCCATAGAGGACCTTT+TGG + chr1_3:63676528-63676547 None:intergenic 50.0%
TTATCTTCTCCCTGCTGAGG+TGG + chr1_3:63676828-63676847 None:intergenic 50.0%
GATAACATGGAGCAGAACCG+TGG - chr1_3:63676843-63676862 Msa0132560:intron 50.0%
!! ATGGATGAAAGTGGTGGTGG+TGG - chr1_3:63676930-63676949 Msa0132560:intron 50.0%
!! TGGTGGTGGTGGTAACTTTG+AGG - chr1_3:63676947-63676966 Msa0132560:intron 50.0%
AGTGGCAAACAAACCTGCAG+TGG - chr1_3:63676971-63676990 Msa0132560:intron 50.0%
GTGGCAAACAAACCTGCAGT+GGG - chr1_3:63676972-63676991 Msa0132560:intron 50.0%
GAGAAGGAGCAGCTGCAAAA+AGG - chr1_3:63677027-63677046 Msa0132560:CDS 50.0%
AGATAGTGCAGGAGTTGTCG+AGG - chr1_3:63677094-63677113 Msa0132560:CDS 50.0%
GAGGAGGAGAGATTCTCTTG+TGG - chr1_3:63677150-63677169 Msa0132560:CDS 50.0%
GAGGGAAATTTGATGGGGGT+TGG - chr1_3:63677216-63677235 Msa0132560:CDS 50.0%
!! TTTGATGGGGGTTGGTTCGT+TGG - chr1_3:63677224-63677243 Msa0132560:CDS 50.0%
!!! GCTGTTTTAAGTGGTGGCGG+TGG - chr1_3:63674110-63674129 Msa0132560:three_prime_UTR 55.0%
TGCACCACCTAACATGACGG+CGG - chr1_3:63674157-63674176 Msa0132560:three_prime_UTR 55.0%
GATGGTGAACGTAGCTTCGG+TGG - chr1_3:63674245-63674264 Msa0132560:three_prime_UTR 55.0%
AAGTTTCTTGTCGCGGCCAC+CGG + chr1_3:63674274-63674293 None:intergenic 55.0%
GTGGCCGCGACAAGAAACTT+TGG - chr1_3:63674274-63674293 Msa0132560:three_prime_UTR 55.0%
AGAACCAAAGATGGCCGAGG+TGG - chr1_3:63675406-63675425 Msa0132560:CDS 55.0%
GGCCGAGGTGGAAAATCAGA+TGG - chr1_3:63675418-63675437 Msa0132560:CDS 55.0%
!! GGAAGAGGGTTGGTGGAAGT+AGG + chr1_3:63675781-63675800 None:intergenic 55.0%
GTCTTCGGAAGACGTCGAGT+AGG + chr1_3:63675854-63675873 None:intergenic 55.0%
GGTCATTGAGAAACGCGAGC+AGG - chr1_3:63675984-63676003 Msa0132560:CDS 55.0%
!! TGTGTTTGTTGTGGTGGCGG+TGG + chr1_3:63676195-63676214 None:intergenic 55.0%
GGCGGTAGCACCAATATCAG+TGG - chr1_3:63676269-63676288 Msa0132560:intron 55.0%
TGGGAGGGATGAGTTCGTCA+AGG - chr1_3:63676430-63676449 Msa0132560:intron 55.0%
GAGGGATGAGTTCGTCAAGG+TGG - chr1_3:63676433-63676452 Msa0132560:intron 55.0%
TGCTCCGGCTGAACCATCAA+CGG + chr1_3:63676795-63676814 None:intergenic 55.0%
!! GATGAAAGTGGTGGTGGTGG+TGG - chr1_3:63676933-63676952 Msa0132560:intron 55.0%
GGTGGGGTCATAGATAGTGC+AGG - chr1_3:63677083-63677102 Msa0132560:CDS 55.0%
TAGTGCAGGAGTTGTCGAGG+AGG - chr1_3:63677097-63677116 Msa0132560:CDS 55.0%
!! GATGGTGGTGATGGTGGTTG+TGG - chr1_3:63677119-63677138 Msa0132560:CDS 55.0%
!! GGTGATGGTGGTTGTGGTTG+TGG - chr1_3:63677125-63677144 Msa0132560:CDS 55.0%
! GATGGTGGTTGTGGTTGTGG+TGG - chr1_3:63677128-63677147 Msa0132560:CDS 55.0%
CCTTCCGCCGTCATGTTAGG+TGG + chr1_3:63674164-63674183 None:intergenic 60.0%
CCACCTAACATGACGGCGGA+AGG - chr1_3:63674161-63674180 Msa0132560:three_prime_UTR 60.0%
CCACCTTCCGCCGTCATGTT+AGG + chr1_3:63674167-63674186 None:intergenic 60.0%
CCTAACATGACGGCGGAAGG+TGG - chr1_3:63674164-63674183 Msa0132560:three_prime_UTR 60.0%
AACATGACGGCGGAAGGTGG+TGG - chr1_3:63674167-63674186 Msa0132560:three_prime_UTR 60.0%
GTAGCTTCGGTGGTAACCGG+TGG - chr1_3:63674255-63674274 Msa0132560:three_prime_UTR 60.0%
CGTCGAGTAGGAGCTAGCCA+TGG + chr1_3:63675842-63675861 None:intergenic 60.0%
AGCACCAACACCAACACCGG+CGG - chr1_3:63676251-63676270 Msa0132560:intron 60.0%
!! ATTGGTGCTACCGCCGGTGT+TGG + chr1_3:63676264-63676283 None:intergenic 60.0%
TTGGGCCCAACTTGGTTGCC+GGG + chr1_3:63676329-63676348 None:intergenic 60.0%
GTTGGGCCCAACTTGGTTGC+CGG + chr1_3:63676330-63676349 None:intergenic 60.0%
!! TGGTGGAGCTGGTTCGAACC+AGG - chr1_3:63676743-63676762 Msa0132560:intron 60.0%
!! TGGAGCTGGTTCGAACCAGG+TGG - chr1_3:63676746-63676765 Msa0132560:intron 60.0%
!! GGCACCGTTGATGGTTCAGC+CGG - chr1_3:63676788-63676807 Msa0132560:intron 60.0%
TGGTTCAGCCGGAGCAGCAA+TGG - chr1_3:63676799-63676818 Msa0132560:intron 60.0%
TTCAGCCGGAGCAGCAATGG+CGG - chr1_3:63676802-63676821 Msa0132560:intron 60.0%
CATGGAGCAGAACCGTGGAC+AGG - chr1_3:63676848-63676867 Msa0132560:intron 60.0%
!! GAAAGTGGTGGTGGTGGTGG+TGG - chr1_3:63676936-63676955 Msa0132560:intron 60.0%
CAAGCTGATGCACCCACTGC+AGG + chr1_3:63676987-63677006 None:intergenic 60.0%
GCAGGAGTTGTCGAGGAGGA+TGG - chr1_3:63677101-63677120 Msa0132560:CDS 60.0%
GGAGTTGTCGAGGAGGATGG+TGG - chr1_3:63677104-63677123 Msa0132560:CDS 60.0%
! GTCGAGGAGGATGGTGGTGA+TGG - chr1_3:63677110-63677129 Msa0132560:CDS 60.0%
!! GAGGAGGATGGTGGTGATGG+TGG - chr1_3:63677113-63677132 Msa0132560:CDS 60.0%
GGTGGTTGTGGTTGTGGTGG+AGG - chr1_3:63677131-63677150 Msa0132560:CDS 60.0%
! GGTTGTGGTTGTGGTGGAGG+AGG - chr1_3:63677134-63677153 Msa0132560:CDS 60.0%
! ATGACGGCGGAAGGTGGTGG+TGG - chr1_3:63674170-63674189 Msa0132560:three_prime_UTR 65.0%
!! GGTGGTGGTGGTGGTGGAGT+TGG - chr1_3:63674182-63674201 Msa0132560:three_prime_UTR 65.0%
!! GCTACCGCCGGTGTTGGTGT+TGG + chr1_3:63676258-63676277 None:intergenic 65.0%
ACCACCCCCGGCAACCAAGT+TGG - chr1_3:63676320-63676339 Msa0132560:intron 65.0%
CCACCCCCGGCAACCAAGTT+GGG - chr1_3:63676321-63676340 Msa0132560:intron 65.0%
TGGGCCCAACTTGGTTGCCG+GGG + chr1_3:63676328-63676347 None:intergenic 65.0%
!! AACGGTGCCGCCATGCTCTC+GGG + chr1_3:63676777-63676796 None:intergenic 65.0%
!! CAACGGTGCCGCCATGCTCT+CGG + chr1_3:63676778-63676797 None:intergenic 65.0%
GAGCATGGCGGCACCGTTGA+TGG - chr1_3:63676779-63676798 Msa0132560:intron 65.0%
CAATGGCGGCCACCTCAGCA+GGG - chr1_3:63676816-63676835 Msa0132560:intron 65.0%
GGTGCAGCTGCTGCTGCTGC+TGG + chr1_3:63674143-63674162 None:intergenic 70.0%
!! ACGGCGGAAGGTGGTGGTGG+TGG - chr1_3:63674173-63674192 Msa0132560:three_prime_UTR 70.0%
!! GCGGAAGGTGGTGGTGGTGG+TGG - chr1_3:63674176-63674195 Msa0132560:three_prime_UTR 70.0%
ACCGCCAGCACAACCACCCC+CGG - chr1_3:63676308-63676327 Msa0132560:intron 70.0%
GTTGCCGGGGGTGGTTGTGC+TGG + chr1_3:63676315-63676334 None:intergenic 70.0%
CCCAACTTGGTTGCCGGGGG+TGG + chr1_3:63676324-63676343 None:intergenic 70.0%
GGGCCCAACTTGGTTGCCGG+GGG + chr1_3:63676327-63676346 None:intergenic 70.0%
TGCTCTCGGGCTGCACCACC+TGG + chr1_3:63676764-63676783 None:intergenic 70.0%
GGTGGTGCAGCCCGAGAGCA+TGG - chr1_3:63676764-63676783 Msa0132560:intron 70.0%
! GGTGCAGCCCGAGAGCATGG+CGG - chr1_3:63676767-63676786 Msa0132560:intron 70.0%
GGTGGCCGCCATTGCTGCTC+CGG + chr1_3:63676810-63676829 None:intergenic 70.0%
GCAATGGCGGCCACCTCAGC+AGG - chr1_3:63676815-63676834 Msa0132560:intron 70.0%
GCCGGGGGTGGTTGTGCTGG+CGG + chr1_3:63676312-63676331 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 63673891 63677478 63673891 ID=Msa0132560;Name=Msa0132560
chr1_3 mRNA 63673891 63677478 63673891 ID=Msa0132560-mRNA-1;Parent=Msa0132560;Name=Msa0132560-mRNA-1;_AED=0.22;_eAED=0.22;_QI=204|1|1|1|1|1|2|471|654
chr1_3 exon 63673891 63676042 63673891 ID=Msa0132560-mRNA-1:exon:21319;Parent=Msa0132560-mRNA-1
chr1_3 exon 63676991 63677478 63676991 ID=Msa0132560-mRNA-1:exon:21318;Parent=Msa0132560-mRNA-1
chr1_3 five_prime_UTR 63677275 63677478 63677275 ID=Msa0132560-mRNA-1:five_prime_utr;Parent=Msa0132560-mRNA-1
chr1_3 CDS 63676991 63677274 63676991 ID=Msa0132560-mRNA-1:cds;Parent=Msa0132560-mRNA-1
chr1_3 CDS 63674362 63676042 63674362 ID=Msa0132560-mRNA-1:cds;Parent=Msa0132560-mRNA-1
chr1_3 three_prime_UTR 63673891 63674361 63673891 ID=Msa0132560-mRNA-1:three_prime_utr;Parent=Msa0132560-mRNA-1
Gene Sequence

>Msa0132560

ATGCTAGGTGACTCAGCTGTTTTAAGTGGTGGCGGTGGAGATGCACCAGCAGCAGCAGCAGCTGCACCACCTAACATGACGGCGGAAGGTGGTGGTGGTGGTGGAGTTGGATCAAATTCAGAGAGTGATGTTGAAAGAGGAAGAGTTGAAGATGGTGAACGTAGCTTCGGTGGTAACCGGTGGCCGCGACAAGAAACTTTGGCGCTTTTAAGCATACGATCGGATATGGATACAGTGTTTCGAGATGCAAGTGTTAAAGGTCCTTTGTGGGATGAAGTTTCAAGGAAGCTAGCAGAACTTGGGTACCACAGAAGTTCAAAGAAATGCAAAGAGAAGTTTGAGAATGTTTATAAGTATCACAAGAGAACCAAAGATGGCCGAGGTGGAAAATCAGATGGTAAAACTTATCGATTTTTTGATCAATTAGAAGCCCTTGATCATTTTCATACCAACCCTTCAACTCATCAACATCAACCTCAAAACATAGCAAAACCACCACAAATTTCGGCACCAACACCGTCACAAGTTGTTACAACTGCAACAGTTTCACTACCAATAACAGAAGCAACAATGTCACAAGTGGTTAACACCACTCTAAGTATCCCTCATGCAACTGTTCCATCAATTTCAATTTCAGTGCCTCAAAACAATATTGCAACTACCCAACCAATTATCAATATGAATCCTACAATCCCATCCAATCCTTCAACTATTTTCCAACCATCAACAACAAACCCTACTTCCACCAACCCTCTTCCATCTTTTCCTAACATTTCTACAGATCTATTATCCAATTCCATGGCTAGCTCCTACTCGACGTCTTCCGAAGACACAACGGAAGAAGGATCTCGAAAGAGGAAGCGGAAATGGAAGGACTTTTTTGAGAGAATAATGAAGAAAGTGACAGAGAAGCAAGAGGATTTGCAGAGGAGATTCTTGGAGGTCATTGAGAAACGCGAGCAGGAACGAGTTGTTAGAGAAGAAGCGTGGAGAGCACAAGAGATGCAAAGAATCAATAGAGAGAGGGAAATGCTTGCACAAGAAAGATCTATAACTGCAGCAAAAGATGCTGCAGTCATGTCTTTCTTGCAAAAAATTGCGGAACAACAAAATCTAGGACAAGCATTGCATAACATCAACATTGCACAACCACCGCCACCACAACAAACACAACAACAACAACAACAACAACCACCACAAAGAAGTGTAGCACCAACACCAACACCGGCGGTAGCACCAATATCAGTGGTACAAGTTAACACAACACCGCCAGCACAACCACCCCCGGCAACCAAGTTGGGCCCAACAATAGTCCCGCAACACCAGCAACAACAATTGGTGACGAATATGGAAATTGTGAAAGTTGATAACAATGGTGAGACTTTCATGGGAGGGATGAGTTCGTCAAGGTGGCCGAAAGTTGAAGTTCAAGCACTGATAAATCTGAGGACGAGCATGGACAACAAGTACCAAGAGAATGGACCAAAAGGTCCTCTATGGGAGGAGATATCTTTAGCAATGAAGAACCTCGGTTACAATAGGAATGCAAAAAGGTGCAAAGAAAAATGGGAGAATATAAACAAGTACTTCAAGAAAGTGAAAGAAAGTAACAAAAAGAGACCCGAAGATTCAAAGACATGTCCTTATTTTCATCAGTTGGATGCTTTGTATAAGGAGAAAGGAAAGGGAGAAAATTCTAGTGGTGGAGCTGGTTCGAACCAGGTGGTGCAGCCCGAGAGCATGGCGGCACCGTTGATGGTTCAGCCGGAGCAGCAATGGCGGCCACCTCAGCAGGGAGAAGATAACATGGAGCAGAACCGTGGACAGGAAGAAGAAGATATGGATGAAGATGATAAAGATGGTGAGGAAGAAGATGATGAAGAAGATATGGATGAAAGTGGTGGTGGTGGTGGTGGTAACTTTGAGGTAGTGGCAAACAAACCTGCAGTGGGTGCATCAGCTTGA
Protein sequence

>Msa0132560

MLGDSAVLSGGGGDAPAAAAAAPPNMTAEGGGGGGVGSNSESDVERGRVEDGERSFGGNRWPRQETLALLSIRSDMDTVFRDASVKGPLWDEVSRKLAELGYHRSSKKCKEKFENVYKYHKRTKDGRGGKSDGKTYRFFDQLEALDHFHTNPSTHQHQPQNIAKPPQISAPTPSQVVTTATVSLPITEATMSQVVNTTLSIPHATVPSISISVPQNNIATTQPIINMNPTIPSNPSTIFQPSTTNPTSTNPLPSFPNISTDLLSNSMASSYSTSSEDTTEEGSRKRKRKWKDFFERIMKKVTEKQEDLQRRFLEVIEKREQERVVREEAWRAQEMQRINREREMLAQERSITAAKDAAVMSFLQKIAEQQNLGQALHNINIAQPPPPQQTQQQQQQQPPQRSVAPTPTPAVAPISVVQVNTTPPAQPPPATKLGPTIVPQHQQQQLVTNMEIVKVDNNGETFMGGMSSSRWPKVEVQALINLRTSMDNKYQENGPKGPLWEEISLAMKNLGYNRNAKRCKEKWENINKYFKKVKESNKKRPEDSKTCPYFHQLDALYKEKGKGENSSGGAGSNQVVQPESMAAPLMVQPEQQWRPPQQGEDNMEQNRGQEEEDMDEDDKDGEEEDDEEDMDESGGGGGGNFEVVANKPAVGASA*