AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0215810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0215810 MtrunA17_Chr2g0317151 95.277 487 17 1 1 487 1 481 0.0 934
Msa0215810 MtrunA17_Chr2g0317131 88.798 491 51 2 1 487 1 491 0.0 859
Msa0215810 MtrunA17_Chr2g0317171 82.413 489 80 3 1 487 1 485 0.0 783
Msa0215810 MtrunA17_Chr2g0317161 81.967 488 78 2 1 487 1 479 0.0 782
Msa0215810 MtrunA17_Chr2g0317221 87.023 393 51 0 95 487 7 399 0.0 669
Msa0215810 MtrunA17_Chr1g0208791 41.775 462 268 1 11 472 16 476 2.10e-126 377
Msa0215810 MtrunA17_Chr6g0483361 42.733 461 248 4 18 472 10 460 2.58e-126 376
Msa0215810 MtrunA17_Chr6g0483381 40.671 477 269 4 11 483 4 470 5.69e-122 365
Msa0215810 MtrunA17_Chr8g0392601 42.105 437 253 0 36 472 9 445 7.65e-121 362
Msa0215810 MtrunA17_Chr1g0208821 41.228 456 266 1 18 473 22 475 2.92e-115 348
Msa0215810 MtrunA17_Chr6g0483341 39.013 446 268 2 39 483 41 483 9.81e-113 342
Msa0215810 MtrunA17_Chr6g0477961 38.994 477 277 4 11 483 4 470 2.26e-109 333
Msa0215810 MtrunA17_Chr6g0483331 38.789 446 269 2 39 483 12 454 1.28e-108 330
Msa0215810 MtrunA17_Chr7g0251331 38.126 459 284 0 14 472 1 459 9.55e-105 321
Msa0215810 MtrunA17_Chr4g0024501 36.688 477 295 1 7 483 39 508 1.40e-104 322
Msa0215810 MtrunA17_Chr6g0483351 42.559 383 206 4 98 472 1 377 3.01e-101 310
Msa0215810 MtrunA17_Chr6g0449061 37.609 460 284 2 16 472 5 464 6.39e-101 311
Msa0215810 MtrunA17_Chr2g0322671 34.064 502 309 2 3 482 2 503 3.79e-100 310
Msa0215810 MtrunA17_Chr1g0208881 37.229 462 285 2 11 472 2 458 1.08e-96 300
Msa0215810 MtrunA17_Chr6g0483161 40.053 377 207 1 96 472 1 358 2.27e-93 288
Msa0215810 MtrunA17_Chr1g0208891 36.446 439 269 2 39 472 24 457 1.88e-89 281
Msa0215810 MtrunA17_Chr5g0442331 35.874 446 285 1 25 470 33 477 2.05e-87 277
Msa0215810 MtrunA17_Chr2g0288281 35.492 386 249 0 98 483 1 386 4.83e-84 264
Msa0215810 MtrunA17_Chr8g0368001 34.023 435 286 1 38 472 49 482 2.69e-81 261
Msa0215810 MtrunA17_Chr5g0412541 33.888 481 304 5 10 483 13 486 8.59e-81 259
Msa0215810 MtrunA17_Chr1g0203791 32.979 470 308 3 13 479 39 504 1.16e-80 260
Msa0215810 MtrunA17_Chr7g0254511 37.868 441 258 4 39 472 30 461 2.69e-80 258
Msa0215810 MtrunA17_Chr1g0202761 35.828 441 278 3 33 472 38 474 1.01e-79 256
Msa0215810 MtrunA17_Chr5g0412771 33.408 446 295 2 38 482 55 499 1.91e-78 254
Msa0215810 MtrunA17_Chr5g0397991 34.023 435 280 3 36 466 62 493 3.03e-75 246
Msa0215810 MtrunA17_Chr7g0254521 34.793 434 282 1 39 472 31 463 4.96e-75 244
Msa0215810 MtrunA17_Chr7g0251321 34.447 389 235 2 98 485 1 370 2.10e-74 239
Msa0215810 MtrunA17_Chr5g0428251 31.070 486 312 6 15 485 35 512 4.51e-73 241
Msa0215810 MtrunA17_Chr6g0468531 33.830 470 294 4 11 472 2 462 4.70e-71 233
Msa0215810 MtrunA17_Chr6g0468521 32.759 464 306 4 11 472 2 461 9.51e-71 233
Msa0215810 MtrunA17_Chr8g0389801 32.895 456 297 3 15 470 6 452 9.85e-71 232
Msa0215810 MtrunA17_Chr6g0473701 31.250 448 295 3 48 485 137 581 2.02e-70 235
Msa0215810 MtrunA17_Chr7g0254531 37.333 375 234 1 98 472 1 374 1.54e-69 227
Msa0215810 MtrunA17_Chr7g0248051 36.674 439 275 2 34 472 66 501 2.61e-68 227
Msa0215810 MtrunA17_Chr1g0154441 35.200 375 242 1 98 472 1 374 4.22e-67 221
Msa0215810 MtrunA17_Chr3g0132531 32.009 453 305 3 21 472 29 479 1.42e-66 222
Msa0215810 MtrunA17_Chr3g0140931 30.357 448 302 4 10 451 20 463 2.48e-66 223
Msa0215810 MtrunA17_Chr6g0468511 33.405 464 303 4 11 472 2 461 8.07e-65 217
Msa0215810 MtrunA17_Chr6g0460541 34.218 377 246 1 96 472 3 377 7.21e-64 213
Msa0215810 MtrunA17_Chr6g0467321 30.853 457 295 6 38 479 41 491 8.82e-64 215
Msa0215810 MtrunA17_Chr5g0412571 36.745 381 233 6 96 472 38 414 8.17e-62 208
Msa0215810 MtrunA17_Chr4g0013031 30.404 421 287 2 39 455 66 484 3.01e-60 206
Msa0215810 MtrunA17_Chr7g0254501 40.067 297 162 4 183 472 2 289 6.73e-57 191
Msa0215810 MtrunA17_Chr4g0025551 59.777 179 49 6 117 295 1 156 7.70e-53 175
Msa0215810 MtrunA17_Chr2g0317211 81.443 97 14 2 1 96 1 94 6.95e-47 158
Msa0215810 MtrunA17_Chr5g0440661 31.165 369 187 7 98 464 1 304 8.64e-45 159
Msa0215810 MtrunA17_Chr3g0132541 26.790 433 261 3 40 472 42 418 4.18e-44 160
Msa0215810 MtrunA17_Chr2g0295631 24.729 461 325 2 38 479 19 476 1.33e-40 152
Msa0215810 MtrunA17_Chr6g0483264 40.000 195 117 0 278 472 1 195 1.49e-40 145
Msa0215810 MtrunA17_Chr8g0364761 30.597 268 185 1 40 306 54 321 5.34e-39 144
Msa0215810 MtrunA17_Chr6g0483241 38.122 181 112 0 96 276 1 181 8.78e-39 139
Msa0215810 MtrunA17_Chr8g0364751 47.742 155 79 2 319 472 13 166 3.16e-33 124
Msa0215810 MtrunA17_Chr6g0487091 26.496 351 226 7 141 472 46 383 3.58e-30 121
Msa0215810 MtrunA17_Chr6g0460521 38.384 198 122 0 275 472 1 198 6.26e-30 116
Msa0215810 MtrunA17_Chr6g0483221 42.568 148 85 0 325 472 6 153 1.05e-29 114
Msa0215810 MtrunA17_Chr5g0398571 31.556 225 148 1 266 484 74 298 1.20e-28 115
Msa0215810 MtrunA17_Chr6g0483261 39.865 148 89 0 325 472 6 153 1.83e-25 102
Msa0215810 MtrunA17_Chr6g0460531 28.452 239 168 2 39 276 24 260 2.63e-21 93.6
Msa0215810 MtrunA17_Chr5g0442311 27.374 179 130 0 98 276 1 179 6.87e-19 84.3
Msa0215810 MtrunA17_Chr5g0398551 42.353 85 49 0 34 118 38 122 1.64e-15 73.6
Msa0215810 MtrunA17_Chr7g0254491 34.615 104 67 1 22 125 14 116 3.98e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0215810 AT3G23560.1 61.359 471 179 1 9 476 5 475 0.0 594
Msa0215810 AT3G23550.1 61.255 462 179 0 13 474 4 465 0.0 585
Msa0215810 AT3G23560.2 61.000 400 153 1 9 405 5 404 3.69e-175 498
Msa0215810 AT5G52450.1 44.470 434 241 0 39 472 25 458 1.02e-116 352
Msa0215810 AT2G34360.1 42.291 454 246 4 20 472 15 453 1.72e-116 351
Msa0215810 AT1G73700.1 41.856 485 263 3 6 482 1 474 1.70e-114 346
Msa0215810 AT1G15150.1 39.565 460 272 3 17 470 2 461 8.21e-107 327
Msa0215810 AT2G04040.1 38.641 471 267 6 11 472 2 459 2.24e-103 317
Msa0215810 AT1G15160.1 38.166 469 284 3 17 479 2 470 2.07e-102 315
Msa0215810 AT1G15170.1 39.780 455 268 2 32 483 23 474 2.42e-102 315
Msa0215810 AT1G66760.1 37.446 462 282 1 11 472 6 460 4.80e-102 313
Msa0215810 AT1G66760.2 37.446 462 282 1 11 472 6 460 5.46e-102 314
Msa0215810 AT2G04070.1 37.660 470 273 5 11 472 2 459 2.79e-100 310
Msa0215810 AT2G04050.1 37.797 463 282 4 11 472 2 459 3.45e-98 304
Msa0215810 AT1G71140.1 37.802 455 270 4 23 472 12 458 3.69e-98 304
Msa0215810 AT1G11670.1 36.261 444 283 0 40 483 50 493 9.30e-98 304
Msa0215810 AT2G04080.1 35.714 476 297 5 11 485 2 469 3.33e-97 301
Msa0215810 AT2G04080.2 36.285 463 289 4 11 472 2 459 8.12e-97 300
Msa0215810 AT2G34360.2 37.528 453 227 2 20 472 15 411 1.36e-96 299
Msa0215810 AT2G04100.2 37.797 463 285 3 11 472 2 462 1.61e-96 300
Msa0215810 AT2G04100.1 38.065 465 281 4 11 472 2 462 1.93e-96 300
Msa0215810 AT3G21690.1 35.426 446 288 0 38 483 50 495 4.02e-95 297
Msa0215810 AT1G15180.1 39.765 425 253 1 32 453 24 448 1.67e-93 292
Msa0215810 AT1G61890.1 34.910 444 289 0 40 483 47 490 6.96e-93 291
Msa0215810 AT2G04090.1 36.853 464 288 4 11 472 2 462 2.52e-92 289
Msa0215810 AT1G61890.2 35.335 433 280 0 40 472 47 479 4.51e-92 289
Msa0215810 AT1G66780.1 35.697 423 271 1 59 481 54 475 6.20e-92 288
Msa0215810 AT4G21903.1 36.471 425 261 2 67 483 80 503 5.01e-90 284
Msa0215810 AT4G21903.2 37.192 406 254 1 67 472 80 484 1.83e-89 283
Msa0215810 AT4G21910.2 34.513 452 289 2 40 485 57 507 5.03e-89 281
Msa0215810 AT4G21910.4 34.513 452 289 2 40 485 57 507 5.03e-89 281
Msa0215810 AT3G59030.1 35.124 484 293 6 7 472 3 483 9.35e-89 281
Msa0215810 AT4G21910.3 34.071 452 291 2 40 485 55 505 3.55e-88 279
Msa0215810 AT4G21910.1 34.071 452 291 2 40 485 55 505 3.55e-88 279
Msa0215810 AT1G64820.1 33.703 451 290 3 36 483 25 469 1.94e-86 275
Msa0215810 AT1G47530.1 36.009 461 292 3 13 472 8 466 3.43e-85 271
Msa0215810 AT1G15170.2 40.517 348 204 1 136 483 11 355 6.38e-85 266
Msa0215810 AT1G61890.3 35.062 405 263 0 40 444 47 451 9.34e-84 266
Msa0215810 AT1G15150.2 41.493 335 196 0 136 470 11 345 1.21e-83 263
Msa0215810 AT1G23300.1 35.104 433 280 1 40 472 44 475 3.80e-83 266
Msa0215810 AT1G33110.1 35.808 458 286 4 16 472 17 467 8.02e-83 265
Msa0215810 AT4G21903.3 35.533 394 246 1 98 483 1 394 4.78e-82 259
Msa0215810 AT1G33090.1 36.518 471 291 4 17 486 18 481 6.68e-82 263
Msa0215810 AT1G33100.1 36.069 463 289 3 10 472 9 464 1.53e-81 261
Msa0215810 AT3G26590.1 33.941 439 278 4 50 482 55 487 6.17e-81 260
Msa0215810 AT1G66760.4 36.709 395 243 1 11 405 6 393 2.50e-80 255
Msa0215810 AT5G44050.1 34.672 473 298 5 3 472 8 472 5.98e-79 254
Msa0215810 AT1G12950.1 33.485 439 280 4 50 482 77 509 2.50e-78 254
Msa0215810 AT5G38030.1 32.952 437 291 2 34 470 40 474 2.36e-77 251
Msa0215810 AT1G47530.2 37.067 375 235 1 98 472 1 374 1.20e-75 243
Msa0215810 AT4G00350.1 34.199 462 296 4 14 472 62 518 9.70e-75 245
Msa0215810 AT3G26590.2 35.038 391 242 4 98 482 1 385 1.42e-74 240
Msa0215810 AT1G33080.2 33.906 466 298 3 10 472 9 467 6.63e-74 241
Msa0215810 AT5G10420.1 35.320 453 280 4 7 453 5 450 7.11e-74 241
Msa0215810 AT1G33080.1 33.906 466 298 3 10 472 9 467 8.24e-74 241
Msa0215810 AT5G65380.1 32.623 469 307 6 7 472 7 469 4.20e-70 231
Msa0215810 AT4G25640.1 33.045 463 305 3 12 472 5 464 3.72e-67 224
Msa0215810 AT4G25640.2 33.045 463 305 3 12 472 5 464 7.59e-67 224
Msa0215810 AT2G38510.1 30.973 452 305 3 39 483 5 456 7.97e-67 223
Msa0215810 AT1G66760.3 35.650 331 213 0 142 472 2 332 2.34e-66 217
Msa0215810 AT5G19700.1 30.238 463 318 2 18 479 17 475 9.83e-66 221
Msa0215810 AT4G29140.1 30.716 433 300 0 38 470 54 486 1.81e-65 220
Msa0215810 AT5G52050.1 29.325 474 317 4 6 470 7 471 1.37e-64 218
Msa0215810 AT1G58340.1 31.294 425 292 0 27 451 45 469 4.01e-64 217
Msa0215810 AT4G23030.1 33.333 489 302 8 12 483 3 484 4.07e-64 216
Msa0215810 AT4G25640.3 32.639 432 286 3 12 441 5 433 2.52e-62 210
Msa0215810 AT5G17700.2 33.410 434 284 5 39 470 33 463 1.32e-61 209
Msa0215810 AT5G17700.1 33.410 434 284 5 39 470 33 463 1.32e-61 209
Msa0215810 AT3G59030.2 32.134 417 262 6 7 405 3 416 4.18e-61 206
Msa0215810 AT5G49130.1 28.482 481 321 3 11 477 6 477 1.37e-60 207
Msa0215810 AT1G71870.1 30.000 460 294 4 11 451 2 452 7.56e-60 205
Msa0215810 AT3G03620.2 31.590 459 302 5 16 470 16 466 8.33e-60 204
Msa0215810 AT3G03620.1 31.590 459 302 5 16 470 16 466 8.33e-60 204
Msa0215810 AT1G33110.2 33.929 392 251 4 16 406 17 401 2.82e-59 201
Msa0215810 AT4G22790.1 30.979 439 297 4 38 472 28 464 1.72e-55 193
Msa0215810 AT1G15180.2 38.889 270 165 0 136 405 11 280 3.38e-55 190
Msa0215810 AT5G17700.3 32.520 369 244 5 39 405 33 398 1.61e-46 167
Msa0215810 AT2G04066.1 35.556 135 79 1 338 472 28 154 2.67e-21 91.3

Find 121 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAGCAAGCATACTCTTTA+AGG 0.063118 2_1:+61335307 Msa0215810:three_prime_UTR
TGAAAACATGAGTTGAATTT+TGG 0.138353 2_1:-61330958 None:intergenic
CTACAAGGTGGTTTCTATTC+TGG 0.187384 2_1:+61335264 Msa0215810:three_prime_UTR
GTGCTACTCTTGCTAATTCT+TGG 0.196457 2_1:+61331072 Msa0215810:CDS
AAACTATAACTTGGAGTTTC+AGG 0.199677 2_1:+61335088 Msa0215810:three_prime_UTR
CAACTTAATGCCTGCCAATT+AGG 0.211751 2_1:+61335005 Msa0215810:three_prime_UTR
TTGTATTTGGCCATGATATA+TGG 0.227753 2_1:+61333900 Msa0215810:CDS
AAATTTGATATATTCATAAA+TGG 0.237367 2_1:-61335374 None:intergenic
CAAGGAGGAGAGAGAGCTTT+AGG 0.245659 2_1:-61333856 None:intergenic
CACAAGAGTTTCCAATGAAT+TGG 0.264220 2_1:+61333790 Msa0215810:CDS
TAAGACCGATTTGCAGTATA+AGG 0.273484 2_1:+61334228 Msa0215810:CDS
CGAACTTATATGGTCCTAAT+TGG 0.291561 2_1:-61335019 None:intergenic
GTCATGCTTGTTGGTCATCT+TGG 0.292545 2_1:+61331032 Msa0215810:CDS
GTTCCAAACACGAACTTATA+TGG 0.294293 2_1:-61335029 None:intergenic
GAAGTTGTTATTTGTGAATC+AGG 0.295836 2_1:-61333348 None:intergenic
GTATATTGAGTTGTACAAAT+TGG 0.301964 2_1:-61330622 None:intergenic
TTCCACCATTTCTCTATTTG+TGG 0.302774 2_1:-61330912 None:intergenic
TTATGCAAGTTTGGAGTATT+GGG 0.305683 2_1:+61333290 Msa0215810:intron
AATAAATTTGTAAGAATCAT+TGG 0.316353 2_1:-61330984 None:intergenic
TCTGTCCAAGGTGTCTTATC+AGG 0.328815 2_1:+61334007 Msa0215810:CDS
CAACTTCATTGATTGCAATA+TGG 0.330876 2_1:+61333364 Msa0215810:CDS
TTAAGTTGTTAAACAACAAT+AGG 0.334821 2_1:-61334990 None:intergenic
TGTGGATTGGTGTGTCAAAC+TGG 0.344629 2_1:+61334894 Msa0215810:CDS
ATTATGCAAGTTTGGAGTAT+TGG 0.350836 2_1:+61333289 Msa0215810:intron
TTGGTTTCTGTCATGCTTGT+TGG 0.351093 2_1:+61331023 Msa0215810:CDS
GGACCATATAAGTTCGTGTT+TGG 0.356095 2_1:+61335026 Msa0215810:three_prime_UTR
TGATAGTCCTATTATCAAAA+AGG 0.356312 2_1:+61333937 Msa0215810:CDS
GTTCATGTTGGAATTGCATA+TGG 0.356912 2_1:+61332616 Msa0215810:CDS
CAATCATTATATCAATTGTT+TGG 0.358665 2_1:+61332407 Msa0215810:CDS
CTGCAACTTCTATTTCACTA+TGG 0.362518 2_1:+61332680 Msa0215810:CDS
CTGTCTCAGGTTGGTTTAAG+TGG 0.365386 2_1:+61332286 Msa0215810:intron
TTGCATATGGATTTGTTCAA+TGG 0.367870 2_1:+61332629 Msa0215810:CDS
AAGGAGGAGAGAGAGCTTTA+GGG 0.372976 2_1:-61333855 None:intergenic
TTGGACAGAATCTAGTAGTA+TGG 0.375092 2_1:-61333993 None:intergenic
CTTGGTGAGCTTCAGTTAGC+TGG 0.379613 2_1:+61331050 Msa0215810:CDS
TGGACTGGTTTAAATTTCAT+AGG 0.381642 2_1:+61332649 Msa0215810:CDS
AATCCAAGGAGACATGAAAT+TGG 0.384730 2_1:-61334205 None:intergenic
TGGATATCAATGATAATGTT+AGG 0.394235 2_1:+61332700 Msa0215810:CDS
ACACTCTGCGGACAAGGATT+TGG 0.397575 2_1:+61332319 Msa0215810:CDS
TTGGCCTGCTCCCAATTCAT+TGG 0.400145 2_1:-61333801 None:intergenic
AAGAGTATGCTTGCTACAAT+AGG 0.408831 2_1:-61335302 None:intergenic
CTAAAGCTCTCTCTCCTCCT+TGG 0.416995 2_1:+61333857 Msa0215810:CDS
ATGATATAGGGTTTATGGAT+TGG 0.419805 2_1:+61334864 Msa0215810:intron
CCATGATCATCTGCTAATAG+AGG 0.432700 2_1:-61330873 None:intergenic
TGGACTAAACTGAATCTCTC+AGG 0.432935 2_1:+61334948 Msa0215810:CDS
TGTCACTGGCGTAGCAGTTA+TGG 0.436994 2_1:+61331100 Msa0215810:CDS
ACAAGAGTTTCCAATGAATT+GGG 0.444602 2_1:+61333791 Msa0215810:CDS
TAAATGAAAATTAGTATGAC+AGG 0.447929 2_1:-61335336 None:intergenic
CAAGACCACAAATAGAGAAA+TGG 0.449315 2_1:+61330907 Msa0215810:CDS
AATTCTTGGTTTAGTGTCAC+TGG 0.452462 2_1:+61331086 Msa0215810:CDS
GAAATGGTGGAACAAAATCT+TGG 0.453708 2_1:+61330923 Msa0215810:CDS
TTACCAATTTCATGTCTCCT+TGG 0.458402 2_1:+61334202 Msa0215810:CDS
CAAAGAAATTTAAGAATACA+TGG 0.465514 2_1:+61332740 Msa0215810:CDS
TTATGGATTGGTCTGATATG+TGG 0.475106 2_1:+61334876 Msa0215810:CDS
GCAAAAGAATATCACATGTT+GGG 0.480088 2_1:+61332343 Msa0215810:CDS
ATTGCTTACATGATCACTTA+CGG 0.481355 2_1:+61333488 Msa0215810:CDS
CGCAAAAGAATATCACATGT+TGG 0.487458 2_1:+61332342 Msa0215810:CDS
GCAGGGGTGGCTAGAGGATG+TGG 0.492719 2_1:+61334127 Msa0215810:intron
AGTTACCTTATACTGCAAAT+CGG 0.494873 2_1:-61334233 None:intergenic
GGTTATACTTTCAGCTATAC+CGG 0.496375 2_1:+61332588 Msa0215810:CDS
TATGGATTTGTTCAATGGAC+TGG 0.501955 2_1:+61332634 Msa0215810:CDS
CTAGAAACACTCTGCGGACA+AGG 0.502058 2_1:+61332313 Msa0215810:CDS
TGCCTTCTGCTGCAATGGTA+TGG 0.502114 2_1:+61332818 Msa0215810:CDS
AAAGCTGAATCCATATATCA+TGG 0.508008 2_1:-61333910 None:intergenic
GTTGTTAAACAACAATAGGT+TGG 0.509921 2_1:-61334986 None:intergenic
ATGCTATATGCAAATAATCC+TGG 0.515013 2_1:-61332514 None:intergenic
GTTTCCAATGAATTGGGAGC+AGG 0.516102 2_1:+61333797 Msa0215810:CDS
AGGATGTGGTTGGCAGCACT+TGG 0.517158 2_1:+61334141 Msa0215810:CDS
GAAAGAGCAAAACATGCAAT+GGG 0.520409 2_1:+61333827 Msa0215810:CDS
CCTCTATTAGCAGATGATCA+TGG 0.522596 2_1:+61330873 Msa0215810:CDS
CTAGTCTTATGTTTGCAAGC+AGG 0.526210 2_1:-61332792 None:intergenic
CTTATATGGTCCTAATTGGC+AGG 0.527480 2_1:-61335015 None:intergenic
AGAAAGAGCAAAACATGCAA+TGG 0.535451 2_1:+61333826 Msa0215810:CDS
GGGTGGCTAGAGGATGTGGT+TGG 0.538894 2_1:+61334131 Msa0215810:CDS
AGCGTTGCCTTCTGCTGCAA+TGG 0.539791 2_1:+61332813 Msa0215810:CDS
GCATCTTGCAAAACTTGTTG+AGG 0.540279 2_1:+61332533 Msa0215810:CDS
TGTGTATGTGTAAATATGTG+TGG 0.548452 2_1:+61330725 Msa0215810:five_prime_UTR
CAATGTCATGAGATTGATGA+AGG 0.548521 2_1:-61332456 None:intergenic
GATTGGTCTGATATGTGGAT+TGG 0.550595 2_1:+61334881 Msa0215810:CDS
TACCATACCATTGCAGCAGA+AGG 0.554425 2_1:-61332820 None:intergenic
TATATGAAGTTTCTTATACC+AGG 0.554707 2_1:+61332496 Msa0215810:CDS
ACTGTGTGATTCAATATGTC+GGG 0.556855 2_1:-61330698 None:intergenic
CACTGTGTGATTCAATATGT+CGG 0.557925 2_1:-61330699 None:intergenic
ATGTTGGGAATTTATCTACA+AGG 0.560309 2_1:+61332358 Msa0215810:CDS
GCAAATCGGTCTTAAATCCA+AGG 0.561263 2_1:-61334219 None:intergenic
ACTGGTTTAAATTTCATAGG+TGG 0.564576 2_1:+61332652 Msa0215810:CDS
GGACTAAACTGAATCTCTCA+GGG 0.566867 2_1:+61334949 Msa0215810:CDS
ATTCCAACATGAACTAATGC+CGG 0.579060 2_1:-61332607 None:intergenic
GTGTAGCAGAACAAATTAAG+AGG 0.579751 2_1:+61330748 Msa0215810:five_prime_UTR
TGGAAAAGACTCTTCTAATG+TGG 0.580347 2_1:+61335120 Msa0215810:three_prime_UTR
TGCATCTAAATGAGCTGCCA+CGG 0.585549 2_1:-61335054 None:intergenic
GACCACAAATAGAGAAATGG+TGG 0.585994 2_1:+61330910 Msa0215810:CDS
GTGGATTGGTGTGTCAAACT+GGG 0.589183 2_1:+61334895 Msa0215810:CDS
ACAATCTGTAGTAATGCCAC+TGG 0.591821 2_1:+61332567 Msa0215810:CDS
ATACCGGCATTAGTTCATGT+TGG 0.592170 2_1:+61332604 Msa0215810:CDS
TGATTCAATATGTCGGGTGG+CGG 0.593779 2_1:-61330692 None:intergenic
TGGCAGCTCATTTAGATGCA+CGG 0.597371 2_1:+61335057 Msa0215810:three_prime_UTR
GTGTGAGCGAAACTATAACT+TGG 0.599994 2_1:+61335079 Msa0215810:three_prime_UTR
ATACTACTAGATTCTGTCCA+AGG 0.600992 2_1:+61333995 Msa0215810:CDS
AATAATGCAGGGGTGGCTAG+AGG 0.604681 2_1:+61334121 Msa0215810:intron
AAATTTAAGAATACATGGAG+AGG 0.607514 2_1:+61332745 Msa0215810:CDS
GCGGCAAGTATCATTTCAGA+TGG 0.611934 2_1:+61330840 Msa0215810:CDS
GTAAGCAATGAATTCTGTGT+TGG 0.613351 2_1:-61333474 None:intergenic
GGAGTTTCAGGCTCAGCGAG+TGG 0.615077 2_1:+61335100 Msa0215810:three_prime_UTR
TTAAACCTGATAAGACACCT+TGG 0.620465 2_1:-61334012 None:intergenic
ACGGTCTTAGTGCAGCTGCA+AGG 0.634465 2_1:+61333507 Msa0215810:CDS
CAAGAGTAAGAAAGTTCACA+TGG 0.640758 2_1:+61330818 Msa0215810:intron
AGTATGGAAATAGCAAGCAA+AGG 0.641984 2_1:-61333977 None:intergenic
GTTCGTGTTTGGAACCGCCG+TGG 0.653954 2_1:+61335037 Msa0215810:three_prime_UTR
GTGTATGTGTAAATATGTGT+GGG 0.654435 2_1:+61330726 Msa0215810:five_prime_UTR
AAATGAAAATTAGTATGACA+GGG 0.663723 2_1:-61335335 None:intergenic
TCAAAACAAAACATAAACCA+AGG 0.664534 2_1:-61333874 None:intergenic
GTGTGATTCAATATGTCGGG+TGG 0.682561 2_1:-61330695 None:intergenic
AGTGAAATAGAAGTTGCAGT+CGG 0.711071 2_1:-61332676 None:intergenic
ATCTAAATGAGCTGCCACGG+CGG 0.715258 2_1:-61335051 None:intergenic
ATAGCTGAAAGTATAACCAG+TGG 0.720921 2_1:-61332583 None:intergenic
AAACAAAACATAAACCAAGG+AGG 0.722868 2_1:-61333871 None:intergenic
TGGATTGGTGTGTCAAACTG+GGG 0.738156 2_1:+61334896 Msa0215810:CDS
GACTAAACTGAATCTCTCAG+GGG 0.747563 2_1:+61334950 Msa0215810:CDS
GAGTAAGAAAGTTCACATGG+CGG 0.753483 2_1:+61330821 Msa0215810:intron
GGTGCACTAGAAACACTCTG+CGG 0.812696 2_1:+61332307 Msa0215810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATCAATTAAAGTAAATT+TGG + chr2_1:61331608-61331627 Msa0215810:intron 10.0%
!!! TTATGTTATTTTGTTATTTA+TGG - chr2_1:61334719-61334738 None:intergenic 10.0%
!! AAATTTGATATATTCATAAA+TGG - chr2_1:61335377-61335396 None:intergenic 10.0%
!! AATAAATTTGTAAGAATCAT+TGG - chr2_1:61330987-61331006 None:intergenic 15.0%
!!! ATTTTATTACTTGATTACTT+TGG + chr2_1:61331004-61331023 Msa0215810:CDS 15.0%
!!! TAGTTTTATAAATTACATGA+AGG + chr2_1:61331677-61331696 Msa0215810:intron 15.0%
!!! ATCATTTTTTTTCTTTCTTT+TGG + chr2_1:61332949-61332968 Msa0215810:intron 15.0%
!! AATATTATACACATATTACA+TGG - chr2_1:61333017-61333036 None:intergenic 15.0%
!!! TATGTGTATAATATTCATTT+TGG + chr2_1:61333022-61333041 Msa0215810:intron 15.0%
!! AAAAAAATGATATATTTGCA+AGG + chr2_1:61333134-61333153 Msa0215810:intron 15.0%
!!! ACTAAAAGTTAATATGTTTT+TGG - chr2_1:61333192-61333211 None:intergenic 15.0%
!! GTAAGATTCATAATATATTA+AGG - chr2_1:61333255-61333274 None:intergenic 15.0%
!!! TATTTTATATTATGCAAGTT+TGG + chr2_1:61333281-61333300 Msa0215810:intron 15.0%
!! AAAAACAATGATTAAACAAA+TGG - chr2_1:61334830-61334849 None:intergenic 15.0%
!!! TTTTGAATGTTTTATGATAT+AGG + chr2_1:61334851-61334870 Msa0215810:intron 15.0%
!!! TTTGAATGTTTTATGATATA+GGG + chr2_1:61334852-61334871 Msa0215810:intron 15.0%
!! ATATCAACAATAAACATCTA+TGG - chr2_1:61331167-61331186 None:intergenic 20.0%
!! TTTATATGTATTCAAGTCAA+TGG + chr2_1:61331449-61331468 Msa0215810:intron 20.0%
!! AATATAGAAATTAGAAACGA+TGG - chr2_1:61331881-61331900 None:intergenic 20.0%
!! CTGAAGAGTTTATATTAATA+AGG - chr2_1:61332085-61332104 None:intergenic 20.0%
!! CTTATTAATATAAACTCTTC+AGG + chr2_1:61332083-61332102 Msa0215810:intron 20.0%
!! CAATCATTATATCAATTGTT+TGG + chr2_1:61332407-61332426 Msa0215810:CDS 20.0%
!! CAAAGAAATTTAAGAATACA+TGG + chr2_1:61332740-61332759 Msa0215810:CDS 20.0%
!! TCAAAACTATTTATCAAACA+AGG - chr2_1:61332903-61332922 None:intergenic 20.0%
!! AAAAAAATGATATACTTGCA+GGG - chr2_1:61332940-61332959 None:intergenic 20.0%
!! AAAAAAAATGATATACTTGC+AGG - chr2_1:61332941-61332960 None:intergenic 20.0%
!!! ATTTTAAACAGAAATCTTGA+AGG - chr2_1:61332977-61332996 None:intergenic 20.0%
!!! TTTGTTTTGATACTTGTATT+TGG + chr2_1:61333887-61333906 Msa0215810:CDS 20.0%
!! ATCATTAACAAAAACAACTT+AGG + chr2_1:61334078-61334097 Msa0215810:intron 20.0%
!! TCATTAACAAAAACAACTTA+GGG + chr2_1:61334079-61334098 Msa0215810:intron 20.0%
!! TATGTTGAGATATAATAAAG+AGG - chr2_1:61334588-61334607 None:intergenic 20.0%
!! ATAGAAATTAGTACATTCAA+TGG + chr2_1:61334671-61334690 Msa0215810:intron 20.0%
!! ATAACAAAATAACATAAGAC+AGG + chr2_1:61334722-61334741 Msa0215810:intron 20.0%
!! TAACAAAATAACATAAGACA+GGG + chr2_1:61334723-61334742 Msa0215810:intron 20.0%
!!! TTAAGTTGTTAAACAACAAT+AGG - chr2_1:61334993-61335012 None:intergenic 20.0%
!! AAATGAAAATTAGTATGACA+GGG - chr2_1:61335338-61335357 None:intergenic 20.0%
!! TAAATGAAAATTAGTATGAC+AGG - chr2_1:61335339-61335358 None:intergenic 20.0%
!! GTATATTGAGTTGTACAAAT+TGG - chr2_1:61330625-61330644 None:intergenic 25.0%
!!! TGAAAACATGAGTTGAATTT+TGG - chr2_1:61330961-61330980 None:intergenic 25.0%
! TATTATAACGTTATGAGTAG+TGG + chr2_1:61331501-61331520 Msa0215810:intron 25.0%
!! ATGTTAGTAATGTTAGTTTC+TGG + chr2_1:61331758-61331777 Msa0215810:intron 25.0%
!! TGTTAGTAATGTTAGTTTCT+GGG + chr2_1:61331759-61331778 Msa0215810:intron 25.0%
! CTAATTTCTATATTCAGAAC+TGG + chr2_1:61331887-61331906 Msa0215810:intron 25.0%
!! TTAGTGGATATACTTTAGTA+AGG - chr2_1:61331993-61332012 None:intergenic 25.0%
! AAAGTATATCCACTAACATA+AGG + chr2_1:61331997-61332016 Msa0215810:intron 25.0%
!!! TGATTTTTTGAGAGAGTTTA+TGG + chr2_1:61332027-61332046 Msa0215810:intron 25.0%
! TTAATCTTGTGACAACATTT+AGG - chr2_1:61332202-61332221 None:intergenic 25.0%
! TATATGAAGTTTCTTATACC+AGG + chr2_1:61332496-61332515 Msa0215810:CDS 25.0%
!! TGGATATCAATGATAATGTT+AGG + chr2_1:61332700-61332719 Msa0215810:CDS 25.0%
! AAATTTAAGAATACATGGAG+AGG + chr2_1:61332745-61332764 Msa0215810:CDS 25.0%
! AAAGGGTTAAATAAAGTGAA+AGG - chr2_1:61332860-61332879 None:intergenic 25.0%
! TGTAAGATCAATCATCAAAA+GGG - chr2_1:61332877-61332896 None:intergenic 25.0%
! TTGTAAGATCAATCATCAAA+AGG - chr2_1:61332878-61332897 None:intergenic 25.0%
!!! TGATTTTAATCACTCCAATA+TGG - chr2_1:61333060-61333079 None:intergenic 25.0%
! CAAAAGTTCTACATATTACA+AGG + chr2_1:61333078-61333097 Msa0215810:intron 25.0%
! TTCTACATATTACAAGGTTT+AGG + chr2_1:61333084-61333103 Msa0215810:intron 25.0%
! TCTACATATTACAAGGTTTA+GGG + chr2_1:61333085-61333104 Msa0215810:intron 25.0%
! TCAAAACAAAACATAAACCA+AGG - chr2_1:61333877-61333896 None:intergenic 25.0%
! TGATAGTCCTATTATCAAAA+AGG + chr2_1:61333937-61333956 Msa0215810:CDS 25.0%
!! TTAAATTCCTCATCTCTTTT+AGG + chr2_1:61334031-61334050 Msa0215810:intron 25.0%
! CATTAACAAAAACAACTTAG+GGG + chr2_1:61334080-61334099 Msa0215810:intron 25.0%
! ATTAACAAAAACAACTTAGG+GGG + chr2_1:61334081-61334100 Msa0215810:intron 25.0%
! AACAATGTGTTAAATAATGC+AGG + chr2_1:61334109-61334128 Msa0215810:intron 25.0%
! ACAATGTGTTAAATAATGCA+GGG + chr2_1:61334110-61334129 Msa0215810:intron 25.0%
!! CAGCTAAATTTGAAATCTAA+GGG - chr2_1:61334432-61334451 None:intergenic 25.0%
!! TCAGCTAAATTTGAAATCTA+AGG - chr2_1:61334433-61334452 None:intergenic 25.0%
! ACCAACGACTATATTAATAA+GGG + chr2_1:61334620-61334639 Msa0215810:intron 25.0%
! GATATCAAGAATAATCATCT+TGG + chr2_1:61334796-61334815 Msa0215810:intron 25.0%
!! GTTTTATGATATAGGGTTTA+TGG + chr2_1:61334859-61334878 Msa0215810:intron 25.0%
!! ATGTAACAAAGTTTTCTACA+AGG + chr2_1:61335249-61335268 Msa0215810:three_prime_UTR 25.0%
! TGGATACAAAGTCTTCTTTT+TGG - chr2_1:61330605-61330624 None:intergenic 30.0%
TGTGTATGTGTAAATATGTG+TGG + chr2_1:61330725-61330744 Msa0215810:five_prime_UTR 30.0%
GTGTATGTGTAAATATGTGT+GGG + chr2_1:61330726-61330745 Msa0215810:five_prime_UTR 30.0%
ATGTGAACTTTCTTACTCTT+GGG - chr2_1:61330819-61330838 None:intergenic 30.0%
TTGATTTATTCTGTCACAGT+TGG - chr2_1:61331596-61331615 None:intergenic 30.0%
! GTTAGTAATGTTAGTTTCTG+GGG + chr2_1:61331760-61331779 Msa0215810:intron 30.0%
! CTATATTCAGAACTGGATTT+TGG + chr2_1:61331894-61331913 Msa0215810:intron 30.0%
TTTAGTAAGGCAATTCAATC+AGG - chr2_1:61331980-61331999 None:intergenic 30.0%
TGACAACATTTAGGTATGTT+TGG - chr2_1:61332193-61332212 None:intergenic 30.0%
GCAAAAGAATATCACATGTT+GGG + chr2_1:61332343-61332362 Msa0215810:CDS 30.0%
ATGTTGGGAATTTATCTACA+AGG + chr2_1:61332358-61332377 Msa0215810:CDS 30.0%
ATGCTATATGCAAATAATCC+TGG - chr2_1:61332517-61332536 None:intergenic 30.0%
! TTGCATATGGATTTGTTCAA+TGG + chr2_1:61332629-61332648 Msa0215810:CDS 30.0%
!! TGGACTGGTTTAAATTTCAT+AGG + chr2_1:61332649-61332668 Msa0215810:CDS 30.0%
! ACTGGTTTAAATTTCATAGG+TGG + chr2_1:61332652-61332671 Msa0215810:CDS 30.0%
TAGGGACTAAAAAAACAACA+AGG + chr2_1:61333103-61333122 Msa0215810:intron 30.0%
! ATTATGCAAGTTTGGAGTAT+TGG + chr2_1:61333289-61333308 Msa0215810:intron 30.0%
! TTATGCAAGTTTGGAGTATT+GGG + chr2_1:61333290-61333309 Msa0215810:intron 30.0%
!! GAAGTTGTTATTTGTGAATC+AGG - chr2_1:61333351-61333370 None:intergenic 30.0%
! CAACTTCATTGATTGCAATA+TGG + chr2_1:61333364-61333383 Msa0215810:CDS 30.0%
! TCATTGATTGCAATATGGTA+TGG + chr2_1:61333369-61333388 Msa0215810:intron 30.0%
ATTGCTTACATGATCACTTA+CGG + chr2_1:61333488-61333507 Msa0215810:CDS 30.0%
! CATTGTTGCATAAATCCTTT+TGG + chr2_1:61333565-61333584 Msa0215810:intron 30.0%
AATGTGATTGTATATGACTG+CGG + chr2_1:61333706-61333725 Msa0215810:intron 30.0%
CAATACATGTACAAGTATGA+AGG - chr2_1:61333744-61333763 None:intergenic 30.0%
ACAAGAGTTTCCAATGAATT+GGG + chr2_1:61333791-61333810 Msa0215810:CDS 30.0%
AAACAAAACATAAACCAAGG+AGG - chr2_1:61333874-61333893 None:intergenic 30.0%
TTGTATTTGGCCATGATATA+TGG + chr2_1:61333900-61333919 Msa0215810:CDS 30.0%
AAAGCTGAATCCATATATCA+TGG - chr2_1:61333913-61333932 None:intergenic 30.0%
!! GCAAACTCCTTTTTGATAAT+AGG - chr2_1:61333947-61333966 None:intergenic 30.0%
AACTTAACCTAAAAGAGATG+AGG - chr2_1:61334041-61334060 None:intergenic 30.0%
CAATGTGTTAAATAATGCAG+GGG + chr2_1:61334111-61334130 Msa0215810:intron 30.0%
CAATGAGATAAAAAGTTGCA+AGG - chr2_1:61334180-61334199 None:intergenic 30.0%
! CTTGCAACTTTTTATCTCAT+TGG + chr2_1:61334178-61334197 Msa0215810:CDS 30.0%
AGTTACCTTATACTGCAAAT+CGG - chr2_1:61334236-61334255 None:intergenic 30.0%
! AATAAGTAGGGGTATACTAA+TGG - chr2_1:61334340-61334359 None:intergenic 30.0%
GTATACCCCTACTTATTAAA+CGG + chr2_1:61334343-61334362 Msa0215810:intron 30.0%
TGTTTCCGTTTAATAAGTAG+GGG - chr2_1:61334351-61334370 None:intergenic 30.0%
GTGTTTCCGTTTAATAAGTA+GGG - chr2_1:61334352-61334371 None:intergenic 30.0%
AGTGTTTCCGTTTAATAAGT+AGG - chr2_1:61334353-61334372 None:intergenic 30.0%
AATGAATGTGTAATCGTGAT+AGG + chr2_1:61334379-61334398 Msa0215810:intron 30.0%
ATGAATGTGTAATCGTGATA+GGG + chr2_1:61334380-61334399 Msa0215810:intron 30.0%
!!! GGTATTCTTTTTTGATGAAG+TGG - chr2_1:61334459-61334478 None:intergenic 30.0%
CTTATTAATATAGTCGTTGG+TGG - chr2_1:61334621-61334640 None:intergenic 30.0%
CACCAACGACTATATTAATA+AGG + chr2_1:61334619-61334638 Msa0215810:intron 30.0%
ACCCTTATTAATATAGTCGT+TGG - chr2_1:61334624-61334643 None:intergenic 30.0%
ATGATATAGGGTTTATGGAT+TGG + chr2_1:61334864-61334883 Msa0215810:intron 30.0%
GTTGTTAAACAACAATAGGT+TGG - chr2_1:61334989-61335008 None:intergenic 30.0%
AAACTATAACTTGGAGTTTC+AGG + chr2_1:61335088-61335107 Msa0215810:three_prime_UTR 30.0%
!!! CTGATATTTTTAAGACGTCT+TGG + chr2_1:61335197-61335216 Msa0215810:three_prime_UTR 30.0%
! TAACAAAGTTTTCTACAAGG+TGG + chr2_1:61335252-61335271 Msa0215810:three_prime_UTR 30.0%
ACTGTGTGATTCAATATGTC+GGG - chr2_1:61330701-61330720 None:intergenic 35.0%
CACTGTGTGATTCAATATGT+CGG - chr2_1:61330702-61330721 None:intergenic 35.0%
GTGTAGCAGAACAAATTAAG+AGG + chr2_1:61330748-61330767 Msa0215810:five_prime_UTR 35.0%
CATGTGAACTTTCTTACTCT+TGG - chr2_1:61330820-61330839 None:intergenic 35.0%
CAAGAGTAAGAAAGTTCACA+TGG + chr2_1:61330818-61330837 Msa0215810:intron 35.0%
CAAGACCACAAATAGAGAAA+TGG + chr2_1:61330907-61330926 Msa0215810:CDS 35.0%
TTCCACCATTTCTCTATTTG+TGG - chr2_1:61330915-61330934 None:intergenic 35.0%
! GAAATGGTGGAACAAAATCT+TGG + chr2_1:61330923-61330942 Msa0215810:CDS 35.0%
AATTCTTGGTTTAGTGTCAC+TGG + chr2_1:61331086-61331105 Msa0215810:CDS 35.0%
! AAGAGTATATGTTCCGAGTA+AGG - chr2_1:61331837-61331856 None:intergenic 35.0%
TTCACTTTGATCTTAGCACA+TGG - chr2_1:61331929-61331948 None:intergenic 35.0%
CGCAAAAGAATATCACATGT+TGG + chr2_1:61332342-61332361 Msa0215810:CDS 35.0%
CAATGTCATGAGATTGATGA+AGG - chr2_1:61332459-61332478 None:intergenic 35.0%
! ATAGCTGAAAGTATAACCAG+TGG - chr2_1:61332586-61332605 None:intergenic 35.0%
GGTTATACTTTCAGCTATAC+CGG + chr2_1:61332588-61332607 Msa0215810:CDS 35.0%
ATTCCAACATGAACTAATGC+CGG - chr2_1:61332610-61332629 None:intergenic 35.0%
GTTCATGTTGGAATTGCATA+TGG + chr2_1:61332616-61332635 Msa0215810:CDS 35.0%
! TATGGATTTGTTCAATGGAC+TGG + chr2_1:61332634-61332653 Msa0215810:CDS 35.0%
AGTGAAATAGAAGTTGCAGT+CGG - chr2_1:61332679-61332698 None:intergenic 35.0%
CTGCAACTTCTATTTCACTA+TGG + chr2_1:61332680-61332699 Msa0215810:CDS 35.0%
!!! GTATTGGGCTTTTGAAGTTT+TGG + chr2_1:61333305-61333324 Msa0215810:CDS 35.0%
!!! GAAGTTTTGGTTTTCTTAGC+TGG + chr2_1:61333318-61333337 Msa0215810:CDS 35.0%
GTAAGCAATGAATTCTGTGT+TGG - chr2_1:61333477-61333496 None:intergenic 35.0%
TCTTAGACTATAGGAAATCC+AGG - chr2_1:61333592-61333611 None:intergenic 35.0%
TGTACAAGTATGAAGGAACT+TGG - chr2_1:61333737-61333756 None:intergenic 35.0%
CACAAGAGTTTCCAATGAAT+TGG + chr2_1:61333790-61333809 Msa0215810:CDS 35.0%
! ATTGCATGTTTTGCTCTTTC+TGG - chr2_1:61333827-61333846 None:intergenic 35.0%
AGAAAGAGCAAAACATGCAA+TGG + chr2_1:61333826-61333845 Msa0215810:CDS 35.0%
GAAAGAGCAAAACATGCAAT+GGG + chr2_1:61333827-61333846 Msa0215810:CDS 35.0%
AGTATGGAAATAGCAAGCAA+AGG - chr2_1:61333980-61333999 None:intergenic 35.0%
TTGGACAGAATCTAGTAGTA+TGG - chr2_1:61333996-61334015 None:intergenic 35.0%
ATACTACTAGATTCTGTCCA+AGG + chr2_1:61333995-61334014 Msa0215810:CDS 35.0%
TTAAACCTGATAAGACACCT+TGG - chr2_1:61334015-61334034 None:intergenic 35.0%
TTACCAATTTCATGTCTCCT+TGG + chr2_1:61334202-61334221 Msa0215810:CDS 35.0%
AATCCAAGGAGACATGAAAT+TGG - chr2_1:61334208-61334227 None:intergenic 35.0%
!! TAAGACCGATTTGCAGTATA+AGG + chr2_1:61334228-61334247 Msa0215810:CDS 35.0%
! CAATTAAAATTTTGCCCGAC+TGG - chr2_1:61334509-61334528 None:intergenic 35.0%
!! CAGTCGGGCAAAATTTTAAT+TGG + chr2_1:61334507-61334526 Msa0215810:intron 35.0%
!! TTATGGATTGGTCTGATATG+TGG + chr2_1:61334876-61334895 Msa0215810:CDS 35.0%
! TTTTGACAAGGCATGTCAAA+TGG + chr2_1:61334928-61334947 Msa0215810:CDS 35.0%
CGAACTTATATGGTCCTAAT+TGG - chr2_1:61335022-61335041 None:intergenic 35.0%
GTTCCAAACACGAACTTATA+TGG - chr2_1:61335032-61335051 None:intergenic 35.0%
TGGAAAAGACTCTTCTAATG+TGG + chr2_1:61335120-61335139 Msa0215810:three_prime_UTR 35.0%
! AAGAGTATGCTTGCTACAAT+AGG - chr2_1:61335305-61335324 None:intergenic 35.0%
TGTAGCAAGCATACTCTTTA+AGG + chr2_1:61335307-61335326 Msa0215810:three_prime_UTR 35.0%
GAGTAAGAAAGTTCACATGG+CGG + chr2_1:61330821-61330840 Msa0215810:intron 40.0%
CCATGATCATCTGCTAATAG+AGG - chr2_1:61330876-61330895 None:intergenic 40.0%
CCTCTATTAGCAGATGATCA+TGG + chr2_1:61330873-61330892 Msa0215810:CDS 40.0%
GACCACAAATAGAGAAATGG+TGG + chr2_1:61330910-61330929 Msa0215810:CDS 40.0%
TTGGTTTCTGTCATGCTTGT+TGG + chr2_1:61331023-61331042 Msa0215810:CDS 40.0%
! GTGCTACTCTTGCTAATTCT+TGG + chr2_1:61331072-61331091 Msa0215810:CDS 40.0%
!! GTGTGTTCATTGATTCGACA+CGG + chr2_1:61331198-61331217 Msa0215810:intron 40.0%
TTATGAGTAGTGGCTCATAC+TGG + chr2_1:61331511-61331530 Msa0215810:intron 40.0%
CACACATGTCCTTATGTTAG+TGG - chr2_1:61332009-61332028 None:intergenic 40.0%
! GCTTTATTCTTCTCTGTCTC+AGG + chr2_1:61332273-61332292 Msa0215810:intron 40.0%
TATTCTTCTCTGTCTCAGGT+TGG + chr2_1:61332277-61332296 Msa0215810:intron 40.0%
!! GCATCTTGCAAAACTTGTTG+AGG + chr2_1:61332533-61332552 Msa0215810:CDS 40.0%
ACAATCTGTAGTAATGCCAC+TGG + chr2_1:61332567-61332586 Msa0215810:CDS 40.0%
ATACCGGCATTAGTTCATGT+TGG + chr2_1:61332604-61332623 Msa0215810:CDS 40.0%
!! CTAGTCTTATGTTTGCAAGC+AGG - chr2_1:61332795-61332814 None:intergenic 40.0%
ACTCCAATATGGTCTACATG+AGG - chr2_1:61333049-61333068 None:intergenic 40.0%
! TGCATAAATCCTTTTGGACC+TGG + chr2_1:61333571-61333590 Msa0215810:intron 40.0%
ATAGGAAATCCAGGTCCAAA+AGG - chr2_1:61333583-61333602 None:intergenic 40.0%
AGCGCGATTTCTTAGACTAT+AGG - chr2_1:61333601-61333620 None:intergenic 40.0%
!! CATGTTTTGCTCTTTCTGGT+TGG - chr2_1:61333823-61333842 None:intergenic 40.0%
TGTGTTAAATAATGCAGGGG+TGG + chr2_1:61334114-61334133 Msa0215810:intron 40.0%
GCAAATCGGTCTTAAATCCA+AGG - chr2_1:61334222-61334241 None:intergenic 40.0%
TGATAGGGTTCACGAATATG+TGG + chr2_1:61334395-61334414 Msa0215810:intron 40.0%
TACAGTTGCGAAAAACAGGT+TGG - chr2_1:61334533-61334552 None:intergenic 40.0%
ACGATACAGTTGCGAAAAAC+AGG - chr2_1:61334537-61334556 None:intergenic 40.0%
!! GATTGGTCTGATATGTGGAT+TGG + chr2_1:61334881-61334900 Msa0215810:CDS 40.0%
TGGACTAAACTGAATCTCTC+AGG + chr2_1:61334948-61334967 Msa0215810:CDS 40.0%
GGACTAAACTGAATCTCTCA+GGG + chr2_1:61334949-61334968 Msa0215810:CDS 40.0%
GACTAAACTGAATCTCTCAG+GGG + chr2_1:61334950-61334969 Msa0215810:CDS 40.0%
CAACTTAATGCCTGCCAATT+AGG + chr2_1:61335005-61335024 Msa0215810:three_prime_UTR 40.0%
CTTATATGGTCCTAATTGGC+AGG - chr2_1:61335018-61335037 None:intergenic 40.0%
GGACCATATAAGTTCGTGTT+TGG + chr2_1:61335026-61335045 Msa0215810:three_prime_UTR 40.0%
GTGTGAGCGAAACTATAACT+TGG + chr2_1:61335079-61335098 Msa0215810:three_prime_UTR 40.0%
CTACAAGGTGGTTTCTATTC+TGG + chr2_1:61335264-61335283 Msa0215810:three_prime_UTR 40.0%
TGATTCAATATGTCGGGTGG+CGG - chr2_1:61330695-61330714 None:intergenic 45.0%
GTGTGATTCAATATGTCGGG+TGG - chr2_1:61330698-61330717 None:intergenic 45.0%
GCGGCAAGTATCATTTCAGA+TGG + chr2_1:61330840-61330859 Msa0215810:CDS 45.0%
! GTCATGCTTGTTGGTCATCT+TGG + chr2_1:61331032-61331051 Msa0215810:CDS 45.0%
GTGGCTCATACTGGAATAAG+AGG + chr2_1:61331520-61331539 Msa0215810:intron 45.0%
! TAGTTTCTGGGGATAGAACC+CGG + chr2_1:61331771-61331790 Msa0215810:intron 45.0%
TATGTTCCGAGTAAGGAGCT+AGG - chr2_1:61331830-61331849 None:intergenic 45.0%
GCAATTCAATCAGGTCTGTC+TGG - chr2_1:61331971-61331990 None:intergenic 45.0%
!! CTGTCTCAGGTTGGTTTAAG+TGG + chr2_1:61332286-61332305 Msa0215810:intron 45.0%
TACCATACCATTGCAGCAGA+AGG - chr2_1:61332823-61332842 None:intergenic 45.0%
GGTCCTCATGTAGACCATAT+TGG + chr2_1:61333043-61333062 Msa0215810:intron 45.0%
CTTTAACACACTCGCTTTCG+CGG + chr2_1:61333542-61333561 Msa0215810:intron 45.0%
TTGTCACTGATCTCGACAGT+TGG - chr2_1:61333688-61333707 None:intergenic 45.0%
GTTTCCAATGAATTGGGAGC+AGG + chr2_1:61333797-61333816 Msa0215810:CDS 45.0%
AAGGAGGAGAGAGAGCTTTA+GGG - chr2_1:61333858-61333877 None:intergenic 45.0%
! TCTGTCCAAGGTGTCTTATC+AGG + chr2_1:61334007-61334026 Msa0215810:CDS 45.0%
!! TGTGGATTGGTGTGTCAAAC+TGG + chr2_1:61334894-61334913 Msa0215810:CDS 45.0%
!! GTGGATTGGTGTGTCAAACT+GGG + chr2_1:61334895-61334914 Msa0215810:CDS 45.0%
!! TGGATTGGTGTGTCAAACTG+GGG + chr2_1:61334896-61334915 Msa0215810:CDS 45.0%
!! GGGCACTCTTACTTTTGACA+AGG + chr2_1:61334916-61334935 Msa0215810:CDS 45.0%
TGCATCTAAATGAGCTGCCA+CGG - chr2_1:61335057-61335076 None:intergenic 45.0%
TGGCAGCTCATTTAGATGCA+CGG + chr2_1:61335057-61335076 Msa0215810:three_prime_UTR 45.0%
!! ATATATAAATTAATCAATAA+GGG - chr2_1:61331342-61331361 None:intergenic 5.0%
!! TATATATAAATTAATCAATA+AGG - chr2_1:61331343-61331362 None:intergenic 5.0%
!!! TTTTAAAATTTTATCTAAAT+TGG - chr2_1:61331641-61331660 None:intergenic 5.0%
!!! TTTTAAAATTTTATCTAAAT+TGG - chr2_1:61331641-61331660 None:intergenic 5.0%
CTTGGTGAGCTTCAGTTAGC+TGG + chr2_1:61331050-61331069 Msa0215810:CDS 50.0%
TGTCACTGGCGTAGCAGTTA+TGG + chr2_1:61331100-61331119 Msa0215810:CDS 50.0%
! GTCACAGTTGGTGCTTTCAG+AGG - chr2_1:61331584-61331603 None:intergenic 50.0%
! AGTTTCTGGGGATAGAACCC+GGG + chr2_1:61331772-61331791 Msa0215810:intron 50.0%
CTAAGGGACGTTGAAGGATG+AGG - chr2_1:61331799-61331818 None:intergenic 50.0%
!! GAGCTAGGTTGGTGAGCTAA+GGG - chr2_1:61331815-61331834 None:intergenic 50.0%
CACCAACCTAGCTCCTTACT+CGG + chr2_1:61331821-61331840 Msa0215810:intron 50.0%
TTCCGAGTAAGGAGCTAGGT+TGG - chr2_1:61331826-61331845 None:intergenic 50.0%
GGTGCACTAGAAACACTCTG+CGG + chr2_1:61332307-61332326 Msa0215810:CDS 50.0%
CTAGAAACACTCTGCGGACA+AGG + chr2_1:61332313-61332332 Msa0215810:CDS 50.0%
ACACTCTGCGGACAAGGATT+TGG + chr2_1:61332319-61332338 Msa0215810:CDS 50.0%
TGCCTTCTGCTGCAATGGTA+TGG + chr2_1:61332818-61332837 Msa0215810:CDS 50.0%
AGTGCAGCTGCAAGGTTAGT+TGG + chr2_1:61333515-61333534 Msa0215810:intron 50.0%
TTGGCCTGCTCCCAATTCAT+TGG - chr2_1:61333804-61333823 None:intergenic 50.0%
CAAGGAGGAGAGAGAGCTTT+AGG - chr2_1:61333859-61333878 None:intergenic 50.0%
CTAAAGCTCTCTCTCCTCCT+TGG + chr2_1:61333857-61333876 Msa0215810:CDS 50.0%
AATAATGCAGGGGTGGCTAG+AGG + chr2_1:61334121-61334140 Msa0215810:intron 50.0%
AGGGTTCACGAATATGTGGC+AGG + chr2_1:61334399-61334418 Msa0215810:intron 50.0%
AGTCACGTGGGTGTTTGTGT+TGG - chr2_1:61334485-61334504 None:intergenic 50.0%
ATCTAAATGAGCTGCCACGG+CGG - chr2_1:61335054-61335073 None:intergenic 50.0%
ACGTTGAAGGATGAGGTCCC+GGG - chr2_1:61331792-61331811 None:intergenic 55.0%
GACGTTGAAGGATGAGGTCC+CGG - chr2_1:61331793-61331812 None:intergenic 55.0%
GGTGAGCTAAGGGACGTTGA+AGG - chr2_1:61331805-61331824 None:intergenic 55.0%
!! GGAGCTAGGTTGGTGAGCTA+AGG - chr2_1:61331816-61331835 None:intergenic 55.0%
AGCGTTGCCTTCTGCTGCAA+TGG + chr2_1:61332813-61332832 Msa0215810:CDS 55.0%
ACGGTCTTAGTGCAGCTGCA+AGG + chr2_1:61333507-61333526 Msa0215810:CDS 55.0%
AGGATGTGGTTGGCAGCACT+TGG + chr2_1:61334141-61334160 Msa0215810:CDS 55.0%
! CGTGGGTGTTTGTGTTGGTC+TGG - chr2_1:61334480-61334499 None:intergenic 55.0%
ACACCCACGTGACTTCCAGT+CGG + chr2_1:61334491-61334510 Msa0215810:intron 55.0%
GGGTGGCTAGAGGATGTGGT+TGG + chr2_1:61334131-61334150 Msa0215810:CDS 60.0%
CACCCACGTGACTTCCAGTC+GGG + chr2_1:61334492-61334511 Msa0215810:intron 60.0%
TGCCCGACTGGAAGTCACGT+GGG - chr2_1:61334497-61334516 None:intergenic 60.0%
TTGCCCGACTGGAAGTCACG+TGG - chr2_1:61334498-61334517 None:intergenic 60.0%
! GTTCGTGTTTGGAACCGCCG+TGG + chr2_1:61335037-61335056 Msa0215810:three_prime_UTR 60.0%
GGAGTTTCAGGCTCAGCGAG+TGG + chr2_1:61335100-61335119 Msa0215810:three_prime_UTR 60.0%
GCAGGGGTGGCTAGAGGATG+TGG + chr2_1:61334127-61334146 Msa0215810:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 61330536 61335426 61330536 ID=Msa0215810;Name=Msa0215810
chr2_1 mRNA 61330536 61335426 61330536 ID=Msa0215810-mRNA-1;Parent=Msa0215810;Name=Msa0215810-mRNA-1;_AED=0.09;_eAED=0.09;_QI=272|1|1|1|0.85|0.75|8|422|487
chr2_1 exon 61330536 61330802 61330536 ID=Msa0215810-mRNA-1:exon:16901;Parent=Msa0215810-mRNA-1
chr2_1 exon 61330832 61331121 61330832 ID=Msa0215810-mRNA-1:exon:16902;Parent=Msa0215810-mRNA-1
chr2_1 exon 61332295 61332839 61332295 ID=Msa0215810-mRNA-1:exon:16903;Parent=Msa0215810-mRNA-1
chr2_1 exon 61333299 61333385 61333299 ID=Msa0215810-mRNA-1:exon:16904;Parent=Msa0215810-mRNA-1
chr2_1 exon 61333472 61333528 61333472 ID=Msa0215810-mRNA-1:exon:16905;Parent=Msa0215810-mRNA-1
chr2_1 exon 61333790 61334028 61333790 ID=Msa0215810-mRNA-1:exon:16906;Parent=Msa0215810-mRNA-1
chr2_1 exon 61334131 61334249 61334131 ID=Msa0215810-mRNA-1:exon:16907;Parent=Msa0215810-mRNA-1
chr2_1 exon 61334873 61335426 61334873 ID=Msa0215810-mRNA-1:exon:16908;Parent=Msa0215810-mRNA-1
chr2_1 five_prime_UTR 61330536 61330802 61330536 ID=Msa0215810-mRNA-1:five_prime_utr;Parent=Msa0215810-mRNA-1
chr2_1 five_prime_UTR 61330832 61330836 61330832 ID=Msa0215810-mRNA-1:five_prime_utr;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61330837 61331121 61330837 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61332295 61332839 61332295 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61333299 61333385 61333299 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61333472 61333528 61333472 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61333790 61334028 61333790 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61334131 61334249 61334131 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 CDS 61334873 61335004 61334873 ID=Msa0215810-mRNA-1:cds;Parent=Msa0215810-mRNA-1
chr2_1 three_prime_UTR 61335005 61335426 61335005 ID=Msa0215810-mRNA-1:three_prime_utr;Parent=Msa0215810-mRNA-1
Gene Sequence

>Msa0215810

ATGGCGGCAAGTATCATTTCAGATGGCACAGAAGCACCTCTATTAGCAGATGATCATGGAAAACAAAACACAAGACCACAAATAGAGAAATGGTGGAACAAAATCTTGGACATAGAAGAAGCCAAAATTCAACTCATGTTTTCATTACCAATGATTCTTACAAATTTATTTTATTACTTGATTACTTTGGTTTCTGTCATGCTTGTTGGTCATCTTGGTGAGCTTCAGTTAGCTGGTGCTACTCTTGCTAATTCTTGGTTTAGTGTCACTGGCGTAGCAGTTATGGTTGGTTTAAGTGGTGCACTAGAAACACTCTGCGGACAAGGATTTGGCGCAAAAGAATATCACATGTTGGGAATTTATCTACAAGGCTCTTGCATTATATCTTTCATTTTTTCAATCATTATATCAATTGTTTGGTTCTACACAGAACACATTCTAGTGTTCCTTCATCAATCTCATGACATTGCTAGAACAGCAGCACTCTATATGAAGTTTCTTATACCAGGATTATTTGCATATAGCATCTTGCAAAACTTGTTGAGGTTTCTACAAACACAATCTGTAGTAATGCCACTGGTTATACTTTCAGCTATACCGGCATTAGTTCATGTTGGAATTGCATATGGATTTGTTCAATGGACTGGTTTAAATTTCATAGGTGGACCGACTGCAACTTCTATTTCACTATGGATATCAATGATAATGTTAGGATTATATGTAATGTATGCAAAGAAATTTAAGAATACATGGAGAGGATTCTCAATGCAATCATTTGATTACCTGCTTGCAAACATAAGACTAGCGTTGCCTTCTGCTGCAATGGTATGTTTGGAGTATTGGGCTTTTGAAGTTTTGGTTTTCTTAGCTGGATTAATGCCTGATTCACAAATAACAACTTCATTGATTGCAATATGTGCCAACACAGAATTCATTGCTTACATGATCACTTACGGTCTTAGTGCAGCTGCAAGCACAAGAGTTTCCAATGAATTGGGAGCAGGCCAACCAGAAAGAGCAAAACATGCAATGGGAGTCACCCTAAAGCTCTCTCTCCTCCTTGGTTTATGTTTTGTTTTGATACTTGTATTTGGCCATGATATATGGATTCAGCTTTTTAGTGATAGTCCTATTATCAAAAAGGAGTTTGCTTCAGTGACACCTTTGCTTGCTATTTCCATACTACTAGATTCTGTCCAAGGTGTCTTATCAGGGGTGGCTAGAGGATGTGGTTGGCAGCACTTGGCTGTTTATGTCAACCTTGCAACTTTTTATCTCATTGGCTTACCAATTTCATGTCTCCTTGGATTTAAGACCGATTTGCAGTATAAGGGTTTATGGATTGGTCTGATATGTGGATTGGTGTGTCAAACTGGGGCACTCTTACTTTTGACAAGGCATGTCAAATGGACTAAACTGAATCTCTCAGGGGACAAAGATAAAGACCAACCTATTGTTGTTTAA
Protein sequence

>Msa0215810

MAASIISDGTEAPLLADDHGKQNTRPQIEKWWNKILDIEEAKIQLMFSLPMILTNLFYYLITLVSVMLVGHLGELQLAGATLANSWFSVTGVAVMVGLSGALETLCGQGFGAKEYHMLGIYLQGSCIISFIFSIIISIVWFYTEHILVFLHQSHDIARTAALYMKFLIPGLFAYSILQNLLRFLQTQSVVMPLVILSAIPALVHVGIAYGFVQWTGLNFIGGPTATSISLWISMIMLGLYVMYAKKFKNTWRGFSMQSFDYLLANIRLALPSAAMVCLEYWAFEVLVFLAGLMPDSQITTSLIAICANTEFIAYMITYGLSAAASTRVSNELGAGQPERAKHAMGVTLKLSLLLGLCFVLILVFGHDIWIQLFSDSPIIKKEFASVTPLLAISILLDSVQGVLSGVARGCGWQHLAVYVNLATFYLIGLPISCLLGFKTDLQYKGLWIGLICGLVCQTGALLLLTRHVKWTKLNLSGDKDKDQPIVV*